mRNA_E_fasciculatus_S2_contig831.16455.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_E_fasciculatus_S2_contig831.16455.1
Unique Name	mRNA_E_fasciculatus_S2_contig831.16455.1
Type	mRNA
Organism	Ectocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)

Homology

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G710_ECTSI)

HSP 1 Score: 139 bits (351), Expect = 6.600e-39
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 2

Query:   95 MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPRT 304
            MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPRT
Sbjct:    1 MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPRT 70

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZC95_9STRA)

HSP 1 Score: 75.5 bits (184), Expect = 3.860e-14
Identity = 39/69 (56.52%), Postives = 52/69 (75.36%), Query Frame = 2

Query:   95 MSTGYRSSKGVLITCDVPTKQFIMSLDARLA--CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVR 295
            MS    S KGVLITCDVPTKQFI+ L+++ A   +++DLD TH+FV+  +V EI+ +LE+L E NVYVR
Sbjct:    1 MSNVRTSRKGVLITCDVPTKQFILHLNSKQAQGFIINDLDETHLFVEASAVHEIREQLERLQEMNVYVR 69

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WPN6_TETOB)

HSP 1 Score: 57.4 bits (137), Expect = 2.060e-7
Identity = 26/67 (38.81%), Postives = 45/67 (67.16%), Query Frame = 2

Query:  113 SSKGVLITCDVPTKQFIMSLD-----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRP 298
            ++ G L+TCDVPTK+F++ L+      + A ++ +LD TH+ VK+D V+ +Q +++  + KNVY  P
Sbjct:    4 ATSGALLTCDVPTKEFLVWLNNQNKATKRAFIIKELDDTHLLVKEDMVEFVQEQVKAFNNKNVYTPP 70

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A6T6HXU1_9STRA)

HSP 1 Score: 58.2 bits (139), Expect = 2.650e-7
Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 2

Query:  113 SSKGVLITCDVPTKQFIMSLDARLAC----VVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 289
            +S G LITCDVPTKQ+I  L+   A     ++ DLDATH+ VKK +  EI+R +E   ++NV+
Sbjct:   36 ASAGYLITCDVPTKQYIQYLNEMKAVDKKFIIEDLDATHLLVKKKARAEIERRVESWMDENVF 98

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IN38_9FLOR)

HSP 1 Score: 58.2 bits (139), Expect = 2.840e-7
Identity = 30/73 (41.10%), Postives = 50/73 (68.49%), Query Frame = 2

Query:   77 TRLRCAMSTGYRSSKGVLITCDVPTKQFIMSLDARLA--CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 289
            TR +  M T   ++ GVLI+CD+P KQF++ +D +     ++SDLD THV VK+ ++  I+ +L++L+E+N Y
Sbjct:   41 TRTQSEMVT---ATAGVLISCDIPVKQFLLHVDEKDTGKFIISDLDETHVLVKESALDYIKSQLDKLYEENQY 110

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2VWH3_GALSU)

HSP 1 Score: 56.6 bits (135), Expect = 3.470e-7
Identity = 27/60 (45.00%), Postives = 44/60 (73.33%), Query Frame = 2

Query:  119 KGVLITCDVPTKQFIMSLDARLA---CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 289
            +GVLITCD+P KQFI+ LD   A    V+++LD T +F++  ++K +Q +LE+L+E+N +
Sbjct:    6 RGVLITCDIPMKQFILKLDEDQAPNRFVIANLDETSLFIQSWALKLVQEKLEELYEENYF 65

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Coccomyxa TaxID=41891 RepID=I0Z2U2_COCSC)

HSP 1 Score: 53.1 bits (126), Expect = 6.560e-6
Identity = 27/65 (41.54%), Postives = 43/65 (66.15%), Query Frame = 2

Query:  119 KGVLITCDVPTKQFIMSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPR 301
            +GVL+ CDVPTK+FI+SL+         V+ DLD T +FV+   V+ +++ L++ +E+N Y  PR
Sbjct:    2 RGVLLQCDVPTKEFILSLNDSKPTNERFVILDLDDTRLFVQPTVVEWLEKRLKEFNEENTYQPPR 66

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PEZ7_PORUM)

HSP 1 Score: 52.0 bits (123), Expect = 1.630e-5
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 2

Query:  113 SSKGVLITCDVPTKQFIMSLDARLA---CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 289
            ++ GVL+TCDVP KQF++ LD   A    +V+ LD TH+F+   +V  I+ +LE+L  +N Y
Sbjct:    4 ATHGVLVTCDVPVKQFLLKLDNDRAPDRFIVAKLDETHLFIHAWAVDMIKDQLERLFAENQY 65

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BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Ostreococcus lucimarinus (strain CCE9901) TaxID=436017 RepID=A4S7H4_OSTLU)

HSP 1 Score: 50.8 bits (120), Expect = 4.910e-5
Identity = 29/63 (46.03%), Postives = 37/63 (58.73%), Query Frame = 2

Query:  122 GVLITCDVPTKQFIMSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRP 298
            GV+IT DVP KQ+I+ L+    A    VV DLDA  V V+ D+V  I RE+E   +   YV P
Sbjct:    7 GVMITADVPIKQYILHLNDVAPAERKFVVVDLDARRVLVQPDAVDAIHREIEAFQQSTQYVAP 69

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Match: General (transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B5Y3U5_PHATC)

HSP 1 Score: 51.6 bits (122), Expect = 5.980e-5
Identity = 29/79 (36.71%), Postives = 45/79 (56.96%), Query Frame = 2

Query:   65 RAPSTRLRCAMSTGYRSSKGVLITCDVPTKQFIMSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 289
            R+P  R +         S G L++CDVPTKQFI  L+         ++ DLDATH+ V+  +  EI+R++E+  + NV+
Sbjct:   20 RSPRHRPKIHNDDDLPPSAGYLVSCDVPTKQFIQHLNDLKPVDKKFILQDLDATHLLVELKARDEIRRKVEEWEDSNVF 98

The following BLAST results are available for this feature:

BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.

BLAST of mRNA_E_fasciculatus_S2_contig831.16455.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 10


Match Name	E-value	Identity	Description
General	6.600e-39	100.00	transcription and DNA repair factor IIH subunit TF...	[more]
General	3.860e-14	56.52	transcription and DNA repair factor IIH subunit TF...	[more]
General	2.060e-7	38.81	transcription and DNA repair factor IIH subunit TF...	[more]
General	2.650e-7	47.62	transcription and DNA repair factor IIH subunit TF...	[more]
General	2.840e-7	41.10	transcription and DNA repair factor IIH subunit TF...	[more]
General	3.470e-7	45.00	transcription and DNA repair factor IIH subunit TF...	[more]
General	6.560e-6	41.54	transcription and DNA repair factor IIH subunit TF...	[more]
General	1.630e-5	43.55	transcription and DNA repair factor IIH subunit TF...	[more]
General	4.910e-5	46.03	transcription and DNA repair factor IIH subunit TF...	[more]
General	5.980e-5	36.71	transcription and DNA repair factor IIH subunit TF...	[more]

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
E_fasciculatus_S2_contig831	contig	E_fasciculatus_S2_contig831:13638..16098 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90	2022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO2	2022-07-07

Properties


Property Name	Value
Stop	1
Start	1
Model size	821
Hectar predicted targeting category	other localisation
Exons	3
Ec32 ortholog description	TFIIH subunit TTDA/Tfb5
Ec32 ortholog	Ec-01_011510.1
Cds size	213

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696842491.4838622-UTR-E_fasciculatus_S2_contig831:13637..13731	1696842491.4838622-UTR-E_fasciculatus_S2_contig831:13637..13731	Ectocarpus fasciculatus EfasUO2	UTR	E_fasciculatus_S2_contig831 13638..13731 +
1696842491.524641-UTR-E_fasciculatus_S2_contig831:15584..16098	1696842491.524641-UTR-E_fasciculatus_S2_contig831:15584..16098	Ectocarpus fasciculatus EfasUO2	UTR	E_fasciculatus_S2_contig831 15585..16098 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696842491.4940667-CDS-E_fasciculatus_S2_contig831:13731..13775	1696842491.4940667-CDS-E_fasciculatus_S2_contig831:13731..13775	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig831 13732..13775 +
1696842491.503605-CDS-E_fasciculatus_S2_contig831:15052..15166	1696842491.503605-CDS-E_fasciculatus_S2_contig831:15052..15166	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig831 15053..15166 +
1696842491.5135648-CDS-E_fasciculatus_S2_contig831:15529..15584	1696842491.5135648-CDS-E_fasciculatus_S2_contig831:15529..15584	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig831 15530..15584 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_E_fasciculatus_S2_contig831.16455.1	prot_E_fasciculatus_S2_contig831.16455.1	Ectocarpus fasciculatus EfasUO2	polypeptide	E_fasciculatus_S2_contig831 13732..15584 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at E_fasciculatus_S2_contig831:13638..16098+
coding sequence from alignment at E_fasciculatus_S2_contig831:13638..16098+

protein sequence of mRNA_E_fasciculatus_S2_contig831.16455.1

>prot_E_fasciculatus_S2_contig831.16455.1 ID=prot_E_fasciculatus_S2_contig831.16455.1|Name=mRNA_E_fasciculatus_S2_contig831.16455.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=71bp
MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVK
EIQRELEQLHEKNVYVRPRT*

mRNA from alignment at E_fasciculatus_S2_contig831:13638..16098+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_E_fasciculatus_S2_contig831.16455.1 ID=mRNA_E_fasciculatus_S2_contig831.16455.1|Name=mRNA_E_fasciculatus_S2_contig831.16455.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2461bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig831:13638..16098+ (Ectocarpus fasciculatus EfasUO2)
GGCAAGCACAAAAAATCCGACAGTCCCGGAAGCAACCAACAACATCCGAG
CACTGACTGAGTACCGAGCACCGAGCACACGGCTGCGTTGTGCGATGAGC
ACAGGATACCGGAGCAGCAAAGGCGTTCTCATCACCTGGTACGGTACGGA
AAGGGAACTGGTCTGTTGCATGCCCTGAGTCGAAGCCTGCCCCAATCATA
GCAGCTTGTCAAGTGATCGCATAGTGGGTCGACTGATGTACATGTACCGT
CCGTAGACTGCTGTAGACAACAGTGTCTAGACGGTGTAGTGCATTAGAGT
ACTGCTACATCAACAGCCTTGTTCTTTCACTGGTAGTTCTATTGCTACTG
TACCGAACTGCCAATGTGGCTCAACATTGCGGGCTACGATACTGACGCTG
GTCATGCCGTTTGTTGCTTAAGATACCACCCCCCTTGGGACCGGTTTACT
CTATGTATGCATACCTACCGGAATACACGTAGCGCAGCCACGTTCAGCAG
CGACGTCGCCGCGGCGGCTTGGGCACCGTCTCGTCGAAAGCTTTCCAAAA
ACGTGCCCTTCGACGGTGGCATCCTCTTCCGTTGTGGAGTAATTGAGCTT
TGGAAAATCTGTCACGGGGGTGTTGCATCTTGTGCAACCTACATCAGGAT
GAACAAAGTTCATTTCTGTATTTGAAACCATGGGCGCCCGTGTTACGGCC
TATTGCATTATTAGGAAAAATTGCATGTAGGGAATGCAGAGAGCGGTGCT
GTTGTTTAACGCGTGTATTTACGCATGCGCGTGAAGTCCACAGGTTGGAA
ATTTGCTGAACTCCTCTTAGAGATGCACATGCGAAGCAGCGGAGCATGTA
GTAATTGGCGGTCTTCTCTTGCAGGCACAGTAGCAGTGGTGATGGCGTTC
CAGAAATGTTTTTTTTTTTCACGGTGACAAACAATTCGCTTTGGCACCTC
AAGGGGGAACCCGCCGTCTACAAAAACATGGCCCAGAACATTGTCGTGGG
GTTGATCTATTTCGCCGCTCTCGTTGTCTTCCCTCCATCGTCGTCGTTCT
CTTGTGCTTGGCATTGCCCGATATAACGTGGTGTTTCACTGTATGTAGCT
GCGCATGTACCATGTAGCTGCGCATGTACCCTGTTTACGTTTTCCCTCTC
AGGCAAAATCAAGGGCAGGGTAGAATCCATTCTATGTCTTTTGAAGAGTC
CGAACTCGTTTTTGTTCAAGTTTTCGGCGCTCGATATCGCTATGATGACT
GGTATTATTCCCCCGCCTCCGCCCCTTCCTACCCAGCGCTGTCCCTCGTT
TTGATGTTCGTGTTGGGTGTTCTGACGCGAAAACGATGGCTGTGTGGACT
TTCGCCGTTTTCGTTGGAGTTGATAACCAACCATGTTGCTTTATTTGTCC
CCTTGTTGTATCCAGCGATGTGCCCACGAAGCAGTTCATTATGAGCCTGG
ACGCACGGCTGGCGTGCGTGGTGTCGGACCTGGACGCCACACATGTCTTC
GTCAAGAAAGACAGCGTCAAAGAGATACAGTGAGTGCTATCCTGCCCACC
TGGTTGTCTGTACAAGTTTGGCAGGAATCACCTTGTAGGAGAAGGCGCGT
GCGTGTGTCGAGCTCTGAAATGAGTTGTTTCAGTTCTATGTTTGCTTTTT
GTATTGTTTTAGAGAAAGCGATGCCGATGTTACGGACTGCGCCGTGGTTG
CACTGTATGTAGTGGGTGTCCTTATCCATATTGTCCAGAAGTTACGCTAC
ATACAATTTATTGCGGAGTGAGTTGTGGACGTGTGCAAGGGGTAAAAGGG
ACATATTATATTTCGGCCGCGGTGATGCTCGCTTTGGGTATACCTAGCCA
TCAACGACCATTCGCCGCATTCCATGCCTCATCCGTTTTCAGGCGCGAGC
TGGAACAGCTGCACGAGAAAAACGTTTACGTGCGGCCTCGAACATAGATA
GCAACAGCGGCGGTACGCAGTAGTGCGCAACAGGAAGCAGAAAGACGGCA
CCGGCACGACGTCTGCAGGGTCGTTGTACTCACCACGCGGACGTTTCATT
TCGCGTGGCAGAGAGCAGGAAAACTGGCGAAGAGAGGGCGGCTTTCAATT
TTTTTTTGTTGGAGCAAGCAGTGTTGAAGGGACGTTGCAGGAGGAAGTCG
TGCAGCTTACTGAGAGGGGGGTTCGCGATCTCTACTGTGCTCGCACTGCT
GTCTGTGTGATCGGCGGTAGTGTGTCTCTGTTCGGATTTCACACCTGGAC
GAGCTATGTCTTGCTTCGACGAGTTTGCAAGCCCCGTACCGTTGATAAGG
TGATGGTACCTCGTTGAAGAAGGGCATTACAGCAGTGTAGTAAAGCGTAC
AATCAATAGGACCGTGCCCAGTTGCACGCAGCATCTCGCAACCATCGTGG
CCATGTGGTGTGGAGATACGTATTGTGGAGGATTGAGACACCCTTGTCAC
TGTAGCATCAT

Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig831:13638..16098+

>mRNA_E_fasciculatus_S2_contig831.16455.1 ID=mRNA_E_fasciculatus_S2_contig831.16455.1|Name=mRNA_E_fasciculatus_S2_contig831.16455.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=213bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig831:13638..16098+ (Ectocarpus fasciculatus EfasUO2)
ATGAGCACAGGATACCGGAGCAGCAAAGGCGTTCTCATCACCTGCGATGT
GCCCACGAAGCAGTTCATTATGAGCCTGGACGCACGGCTGGCGTGCGTGG
TGTCGGACCTGGACGCCACACATGTCTTCGTCAAGAAAGACAGCGTCAAA
GAGATACAGCGCGAGCTGGAACAGCTGCACGAGAAAAACGTTTACGTGCG
GCCTCGAACATAG