mRNA_E_fasciculatus_S2_contig819.16336.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig819.16336.1
Unique NamemRNA_E_fasciculatus_S2_contig819.16336.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: Heat (shock protein, mitochondrial protein import, putative mitochondrial translocase complex subunit, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQE4_ECTSI)

HSP 1 Score: 288 bits (736), Expect = 6.490e-90
Identity = 177/201 (88.06%), Postives = 184/201 (91.54%), Query Frame = 1 
Query:  142 NGGRSSGSNRGLLLQPRQRSSCSVRGNTCRPLNAGFGQRDDSXXXXXXXXXXXXXXXXXXXXXXXXXXXX--VPDAVDEKGTGQRQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTPDEVTKGRAPDLTPEIIDKYDVAMGEDGVLTLSPKDGGVGEEGEETPSS 738
            NGGRSSGS+RG+LL P QRSS S R NTCRPLNAGFGQRDD XXXXXXXXXXXXXXXXXXXXXXXXXXXX  VP+A   KGTGQRQLFMQFTCN CEGVSQYMINKNAYEEGIVI TCQSCGA+HLIADNLKKLDFP FGNNIEEYMQSTGTPDEVTKGRAPDLTPEI +K+DVAMG+DGVLTLSPKDG VGEEGEE PSS
Sbjct:  463 NGGRSSGSSRGVLLHPTQRSSSSARSNTCRPLNAGFGQRDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPEAEGAKGTGQRQLFMQFTCNLCEGVSQYMINKNAYEEGIVICTCQSCGARHLIADNLKKLDFPAFGNNIEEYMQSTGTPDEVTKGRAPDLTPEIAEKFDVAMGKDGVLTLSPKDGAVGEEGEEPPSS 663
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: DNL-type (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L228_9PHAE)

HSP 1 Score: 200 bits (509), Expect = 1.580e-61
Identity = 149/183 (81.42%), Postives = 156/183 (85.25%), Query Frame = 1 
Query:    1 MKTMVPVTLGATLLGIHFTGNLQAEGFVVVPVGE-RDSQQXXXXXXXXNGGRS--SGSNRGLLLQPRQRSSCSVRGNTCRPLNAGFGQRDDSXXXXXXXXXXXXXXXXXXXXXXXXXXXX-VPDAVDEKGTGQRQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKL 537
            MKTMVPV+LGATLLG+H TGN Q +GFVVVPVG  R    XXXXXXXX   RS  SGS RG LLQP QRSSCS R NTCRPLNAGFGQ+D  XXXXXXXXXXXXXXXXXXXXXXXXXXXX VP+A   KGTGQRQLFMQFTCN CEGVSQYMINKNAYEEGIVI TCQSCGA+HLIADNLKK+
Sbjct:    1 MKTMVPVSLGATLLGLHLTGNRQVDGFVVVPVGSSRPXXXXXXXXXXXXXXRSGSSGSGRGALLQPTQRSSCSARSNTCRPLNAGFGQQDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPEAEGAKGTGQRQLFMQFTCNLCEGVSQYMINKNAYEEGIVICTCQSCGARHLIADNLKKV 183
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: DNL-type (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHA9_9PHAE)

HSP 1 Score: 89.0 bits (219), Expect = 1.800e-17
Identity = 37/69 (53.62%), Postives = 50/69 (72.46%), Query Frame = 1 
Query:  388 RQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTP 594
            R++ + FTCN C+G + + IN+ ++ +GIV+  CQ C  KHLIADNL KLDFP FG N+EE+M   GTP
Sbjct:   96 RKITLSFTCNVCDGHNTHQINRVSFMQGIVVLWCQHCNQKHLIADNLGKLDFPEFGRNLEEFMAKKGTP 164
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: DNL-type (domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLU7_9STRA)

HSP 1 Score: 79.7 bits (195), Expect = 1.200e-15
Identity = 34/65 (52.31%), Postives = 46/65 (70.77%), Query Frame = 1 
Query:  394 LFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTG 588
            + + FTC +C+  + Y I++ AY +GIVI TC  C ++HLIADN   LDFP FG NIE+YM+S G
Sbjct:    1 MTLAFTCKKCDTRNIYKISRVAYYQGIVICTCSGCQSRHLIADNKGLLDFPEFGRNIEDYMESQG 65
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: DNL-type (domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CWU7_9STRA)

HSP 1 Score: 80.5 bits (197), Expect = 3.250e-14
Identity = 43/118 (36.44%), Postives = 66/118 (55.93%), Query Frame = 1 
Query:  388 RQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTP-------DEVTKGRAPDL-------TPEIIDKYDVAMGEDGVLTLSPKD 699
            R+  M +TCN CEG +   +++ A+ EGIV+A C+ C AKHL+ADNL KLD   FG  +++  +    P       D++   R  +         PE+  KYD+ + EDG + L+PK+
Sbjct:  170 RKFQMLYTCNICEGRNLIQVDRVAFTEGIVVARCKHCDAKHLVADNLSKLD---FGGLVKKGKEGESNPAGGKVTLDDILAARGEEAKYLDIKSNPELAAKYDLMVLEDGTVELAPKE 284
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: DNL-type (domain-containing protein n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FM48_9STRA)

HSP 1 Score: 66.2 bits (160), Expect = 8.260e-10
Identity = 33/68 (48.53%), Postives = 45/68 (66.18%), Query Frame = 1 
Query:  400 MQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGN-NIEEYMQSTGTPDE 600
            +Q+TCN CE  ++ ++++ AY EG+VIA C+ C AKH IADNL     PT  + NIEEY  S G  +E
Sbjct:   77 LQYTCNVCEHRNRVIVSRMAYREGVVIAICKGCTAKHWIADNLD----PTLSDTNIEEYFVSQGKKEE 140
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: DNL-type (zinc finger protein-like n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1D7110)

HSP 1 Score: 64.7 bits (156), Expect = 3.130e-9
Identity = 35/86 (40.70%), Postives = 48/86 (55.81%), Query Frame = 1 
Query:  385 QRQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTPDEVTKGRAPDLTPEII 642
            + + F+ +TC  C   + +M +K AYEEGIVI TC+ C  KHLIADNL   +      NIEE ++  G      K  + D+  EII
Sbjct:   83 EAKKFLAYTCKVCNTRNSHMFSKKAYEEGIVIVTCEGCKNKHLIADNLGWFEH-VGKRNIEEILEERGEQARYVKTDSDDI--EII 165
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: Dnl-type (zinc finger n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7U207_9STRA)

HSP 1 Score: 65.9 bits (159), Expect = 6.320e-9
Identity = 30/69 (43.48%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  388 RQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTP 594
            R+  M +TCN CEG +   +++ A+ EGIV+A C+ C AKHL+ADNL KLD   FG  +++  +    P
Sbjct:  185 RKFQMLYTCNICEGRNLIQVDRVAFTEGIVVARCKHCDAKHLVADNLSKLD---FGGLVKKGKEGESNP 250
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BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: Ubiquinone (biosynthesis protein COQ4 homolog, mitochondrial n=2 Tax=Onchocerca flexuosa TaxID=387005 RepID=A0A183I2N4_9BILA)

HSP 1 Score: 60.8 bits (146), Expect = 3.060e-7
Identity = 34/80 (42.50%), Postives = 46/80 (57.50%), Query Frame = 1 
Query:  379 TGQRQLFMQFTCNRCEGVSQ--YMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTPDEVTKG 612
            + Q +L + +TC  C G  Q   + +K AYEEG+VI TC+SC   HLIADNL          NIEE ++  G  +E+ KG
Sbjct:   63 SSQGRLSLSYTCKVC-GTRQGPKLFSKKAYEEGVVIVTCESCKNHHLIADNLGWFPQSKNSRNIEEILEKKG--EEIRKG 139
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Match: Mitochondrial (import protein TIM15 n=2 Tax=Ostreococcus tauri TaxID=70448 RepID=Q01GH6_OSTTA)

HSP 1 Score: 58.5 bits (140), Expect = 7.570e-7
Identity = 27/86 (31.40%), Postives = 46/86 (53.49%), Query Frame = 1 
Query:  370 EKGTGQRQLFMQFTCNRCEGVSQYMINKNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQST-----GTPDEVTKG 612
            E    ++ L+M FTC RC+  +    ++ AYE+G+VI TC  C  KH++AD +     P    ++E+++        G  D+ T+G
Sbjct:   85 ENANARKDLYMMFTCGRCDARAARGFSRQAYEKGVVIVTCPECQVKHVVADRMGWFGEP---GSVEDFIAEKARADGGGEDKATRG 167
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig819.16336.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Heat6.490e-9088.06shock protein, mitochondrial protein import, putat... [more]
DNL-type1.580e-6181.42domain-containing protein n=1 Tax=Ectocarpus sp. C... [more]
DNL-type1.800e-1753.62domain-containing protein n=1 Tax=Ectocarpus sp. C... [more]
DNL-type1.200e-1552.31domain-containing protein n=1 Tax=Tribonema minus ... [more]
DNL-type3.250e-1436.44domain-containing protein n=1 Tax=Nannochloropsis ... [more]
DNL-type8.260e-1048.53domain-containing protein n=1 Tax=Fragilariopsis c... [more]
DNL-type3.130e-940.70zinc finger protein-like n=1 Tax=Mercenaria mercen... [more]
Dnl-type6.320e-943.48zinc finger n=1 Tax=Nannochloropsis gaditana TaxID... [more]
Ubiquinone3.060e-742.50biosynthesis protein COQ4 homolog, mitochondrial n... [more]
Mitochondrial7.570e-731.40import protein TIM15 n=2 Tax=Ostreococcus tauri Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig819contigE_fasciculatus_S2_contig819:4556..6706 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score290.8
Seed ortholog evalue6e-76
Seed eggNOG ortholog2880.D7FQE4
Model size741
Hectar predicted targeting categorysignal peptide
Exons4
EggNOG free text desc.ankyrin repeats
EggNOG OGs28PNG@1,2QWAI@2759
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-01_006425.1
Cds size741
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842479.8672118-CDS-E_fasciculatus_S2_contig819:4555..47621696842479.8672118-CDS-E_fasciculatus_S2_contig819:4555..4762Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig819 4556..4762 -
1696842479.8799624-CDS-E_fasciculatus_S2_contig819:5145..53701696842479.8799624-CDS-E_fasciculatus_S2_contig819:5145..5370Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig819 5146..5370 -
1696842479.892722-CDS-E_fasciculatus_S2_contig819:5997..61951696842479.892722-CDS-E_fasciculatus_S2_contig819:5997..6195Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig819 5998..6195 -
1696842479.9031687-CDS-E_fasciculatus_S2_contig819:6595..67061696842479.9031687-CDS-E_fasciculatus_S2_contig819:6595..6706Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig819 6596..6706 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig819.16336.1prot_E_fasciculatus_S2_contig819.16336.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig819 4556..6706 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig819.16336.1

>prot_E_fasciculatus_S2_contig819.16336.1 ID=prot_E_fasciculatus_S2_contig819.16336.1|Name=mRNA_E_fasciculatus_S2_contig819.16336.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=247bp
MKTMVPVTLGATLLGIHFTGNLQAEGFVVVPVGERDSQQQQRACCARNGG
RSSGSNRGLLLQPRQRSSCSVRGNTCRPLNAGFGQRDDSAAAAKSSKKKG
KGKKAAKQGGGAAGGGGVPDAVDEKGTGQRQLFMQFTCNRCEGVSQYMIN
KNAYEEGIVIATCQSCGAKHLIADNLKKLDFPTFGNNIEEYMQSTGTPDE
VTKGRAPDLTPEIIDKYDVAMGEDGVLTLSPKDGGVGEEGEETPSS*
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mRNA from alignment at E_fasciculatus_S2_contig819:4556..6706-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig819.16336.1 ID=mRNA_E_fasciculatus_S2_contig819.16336.1|Name=mRNA_E_fasciculatus_S2_contig819.16336.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2151bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig819:4556..6706- (Ectocarpus fasciculatus EfasUO2)
ATGAAGACCATGGTGCCAGTAACGTTGGGCGCGACCTTGCTAGGAATCCA
CTTCACCGGCAACCTCCAGGCAGAAGGTTTCGTCGTAGTCCCAGTCGGTG
AGCGCGACAGCGTCAGCATGTTGTACCCTGATCCTCCGGACGGGACTGTC
AGCTAGAATCAGGGTCCTGCTACGCCGTGCCCACAGCAGTTGCAGGTGCT
TCCTTGCTGTTGATTGAGAGACGTCGAGACTAACTCTGTTCGAGACATGT
GCACTGCGCACGACAAGATGGCGAGGAAGATTAGGTTTCGATCGATTCGA
ATGCTAGATGCATGGCTCAAGCGACGTGATACTGCTGTGCTGTGTGCGCC
AAAAAAGGGGCATGACACGCTGACCATCGCACGTTTCACACGAGTTCGGA
GGTTTTGACGCACACCACCAACTGCGATCGCACTCACCACGCGGCGGCAA
CGGCCATCGTTGCCCAGGGTCTTCCCGACCCTCGCAGCAGCAGCTGCAGC
AGCAGCAGCAGCAGCAGCAGCAGAGGGCATGCTGTGCCAGGAACGGCGGT
CGCAGCAGTGGCAGCAACCGAGGATTATTGCTGCAGCCGAGACAACGCTC
GTCCTGCTCGGTTCGAGGCAACACGTGTCGGCCGTTGAACGCTGGGTTCG
GGCAGCGGGATGACTCGGCAGCAGCAGCGAAGTCTTCGAAGAAGAAAGGG
AAAGGAAAGGTGCGCCGAGAGCGCGACGGTTGGTTTGCTGGTTGCATGAT
TTACTGCACCAAGGGATTTGTTCGGTACCCTTCTGGAGAGTATGGAGTTC
GCGCGCGGCCATATTGATCTGGTGCCCTTGGGTGCGTTGTGGAGTTATCA
TGCGGCCACAGAGAGCGTCCGCGTCAGGTGGATATTGTAGCACCAGTGTT
TACGAGTGTCAGAGGGTACTGAGCAGAGATCGGAACGGTTTTCAATGTGA
AGACAAGAACAGCAAGGAGGCACGGTGAGCTTGAATATATTTCTGGCGGT
TGAGGCGTCATGACCGTCGACGCAACACGAGGAGGTTGGTTACACAACGT
GGATCACCAGTGTACTGCTGCTAATGTATATTTACGGGTTTTGTTGGCCC
CGCATGTATAGAAGAAAGTGGTTTGGTTTGCTTCTTTTTGTTTGCCTCTT
GACACCCTCCATCGGTTCGCGTGTGTGAGTGCAGCCAGCACCTTGTTGGT
ACTCTTGTACTTGTGCCGATTGAGTGTGCCGGTAGCGCCTGCTGGGTGTT
GATCGTCTGCTCCTTTCCCCCTCCATCTTCCCCCACGGATACTGCATCCA
TGCGCTGCTGGGCTTCGGATTCGATTCGCCGAGTAGAAAGCAGCGAAACA
GGGAGGCGGGGCTGCCGGGGGTGGGGGAGTTCCAGATGCAGTAGACGAGA
AGGGCACTGGGCAACGGCAGCTTTTCATGCAGTTCACGTGCAATCGGTGT
GAAGGGGTGAGCCAATACATGATCAACAAGAACGCCTACGAGGAAGGCAT
CGTGATCGCCACGTGCCAGTCCTGCGGGGCGAAGCACCTCATCGCAGACA
ACTTGAAGAAGGTGAGATGCTGTGGTCCTAAAATGGTCTCTGATCAGCCT
TTTTCCCTACCCTTAATCATGGTAGACTACACACACTCCTGCGCGGGTAC
ACGAAGCTCGGTGATATCGGCGATGTCGCGGCGCAAACATATTGTCCTGA
TCATGCAACACTCCAAATCTAGGCACCATGGTGCACTAGATATCACACAC
GGTGGTGGCTTGTTTAATCCCGATTTTTAGCGGGGCTCATTGTCTTGCGT
GCCCACACGTCAGGAAAAAAGCCCTCACTAACATGACTATTGGTCAGATA
CCTGTAACATTCCGTTCCAGCATTATTTTTCCTCGCCACGCTTGACTGCC
CCTGCCTGCGTTGTCCCGTTTCACGTTTGTTTTATCCCGCGTAGTTGGAC
TTTCCCACTTTCGGAAACAACATCGAGGAGTACATGCAGTCCACCGGGAC
GCCGGACGAGGTGACCAAGGGCAGAGCGCCCGACCTCACTCCCGAGATCA
TCGACAAGTACGACGTGGCCATGGGCGAAGACGGGGTCCTCACTCTCTCG
CCGAAGGACGGCGGCGTCGGCGAAGAAGGCGAAGAGACTCCTTCTTCGTG
A
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig819:4556..6706-

>mRNA_E_fasciculatus_S2_contig819.16336.1 ID=mRNA_E_fasciculatus_S2_contig819.16336.1|Name=mRNA_E_fasciculatus_S2_contig819.16336.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=741bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig819:4556..6706- (Ectocarpus fasciculatus EfasUO2)
ATGAAGACCATGGTGCCAGTAACGTTGGGCGCGACCTTGCTAGGAATCCA
CTTCACCGGCAACCTCCAGGCAGAAGGTTTCGTCGTAGTCCCAGTCGGTG
AGCGCGACAGCCAGCAGCAGCAGAGGGCATGCTGTGCCAGGAACGGCGGT
CGCAGCAGTGGCAGCAACCGAGGATTATTGCTGCAGCCGAGACAACGCTC
GTCCTGCTCGGTTCGAGGCAACACGTGTCGGCCGTTGAACGCTGGGTTCG
GGCAGCGGGATGACTCGGCAGCAGCAGCGAAGTCTTCGAAGAAGAAAGGG
AAAGGAAAGAAAGCAGCGAAACAGGGAGGCGGGGCTGCCGGGGGTGGGGG
AGTTCCAGATGCAGTAGACGAGAAGGGCACTGGGCAACGGCAGCTTTTCA
TGCAGTTCACGTGCAATCGGTGTGAAGGGGTGAGCCAATACATGATCAAC
AAGAACGCCTACGAGGAAGGCATCGTGATCGCCACGTGCCAGTCCTGCGG
GGCGAAGCACCTCATCGCAGACAACTTGAAGAAGTTGGACTTTCCCACTT
TCGGAAACAACATCGAGGAGTACATGCAGTCCACCGGGACGCCGGACGAG
GTGACCAAGGGCAGAGCGCCCGACCTCACTCCCGAGATCATCGACAAGTA
CGACGTGGCCATGGGCGAAGACGGGGTCCTCACTCTCTCGCCGAAGGACG
GCGGCGTCGGCGAAGAAGGCGAAGAGACTCCTTCTTCGTGA
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