mRNA_E_fasciculatus_S2_contig8144.16299.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig8144.16299.1
Unique NamemRNA_E_fasciculatus_S2_contig8144.16299.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: C2H2 (zinc finger protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWN0_ECTSI)

HSP 1 Score: 308 bits (789), Expect = 1.280e-95
Identity = 249/310 (80.32%), Postives = 258/310 (83.23%), Query Frame = 3 
Query:   96 MSRPPTMSQLLKRAGLAKRPRPAGRGVDATPSIANQSNKKRSRVDGGSRQHSSQEXXXXXXXXXXXXXXXSRNENGASAKDVAVDDQIAALERELKGTDGSSSDADSXXXXXXXXXXXXXXXXXXXSRGAGRKRGRVEKLVSPLEAEKIEPLPAHLLPRAGCGVHKDKXXXXXXXXXXXXXXXXXVPGPSQGLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLHVEAAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEKLQSTMSRQGKWVPGGRGGSESEGSSNGYSGRENG 1025
            MS PPTMSQLLKRAGLAKRPRPAGRGVDATPS A+QS +KR RVD           XXXXXXXXXXXXXX R+ENG SAKDVAVDDQIAALERELKG + SSSDA+ XXXXXXXXXXXXX       RGAGR+RGRV KLVSPLE EKIEPLPAHLLPR GCGVHKDK XXXXX           +PGPSQGLDSAVKEL+ NYEARSSERVPFYCRVCKFQGDSLEALEKHK EPLHV    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEKLQSTMSRQGKWVPGG GG  SE S+NGYSGRENG
Sbjct:    1 MSGPPTMSQLLKRAGLAKRPRPAGRGVDATPSTASQSKRKRPRVDXXXXXXXX---XXXXXXXXXXXXXXGRSENGTSAKDVAVDDQIAALERELKGANDSSSDAEXXXXXXXXXXXXXX-------RGAGRERGRVAKLVSPLEVEKIEPLPAHLLPRPGCGVHKDKKXXXXXPSAAVGTTA--LPGPSQGLDSAVKELLANYEARSSERVPFYCRVCKFQGDSLEALEKHKEEPLHVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEKLQSTMSRQGKWVPGGWGGPGSERSANGYSGRENG 298
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: C2H2-type (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KMP8_9PHAE)

HSP 1 Score: 306 bits (785), Expect = 4.880e-95
Identity = 263/369 (71.27%), Postives = 268/369 (72.63%), Query Frame = 3 
Query:   96 MSRPPTMSQLLKRAGLAKRPRPAGRGVDATPSIANQSNKKRSRVDGGSRQHSSQEXXXXXXXXXXXXXXXSRNENGASAKDVAVDDQIAALERELKGTDGSSSDADSXXXXXXXXXXXXXXXXXXXSRGAGRKRGRVEKLVSPLEAEKIEPLPAHLLPRAGCGVHKDKXXXXXXXXXXXXXXXXXVPGPSQGLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLHVEAAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEKLQSTMSRQGKWVPGGRGGSESEGSSNGYSGRENGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGRGDPSRXXXXXXXXPGRGYERDR 1202
            M RPPTMSQLLKRAGLAKR RPAGRGVDATPS ANQSN KR RVDG          XXXXXXXXXXXXXXSR+ENGASAKDV VDDQIAALERELKG D SSSDA+S    XXXXXXXXXXXXXXX      +RGR  KLVSPLEAEKIEPLPAHLLPR GCGVHKD                  +PGPSQGLDSAVKEL+ NYEARSSERVPFYCRVCKFQGDSLEALEKHK EPLHV    XXXXXXXXXXXXXXXXXXXXXXXXXX    LEKLQS MSRQGKWVPGGRGG  SEGS NGYSGRENG                               XXXX    PSR  XXXXXXPGRGYE DR
Sbjct:    1 MFRPPTMSQLLKRAGLAKRSRPAGRGVDATPSPANQSNNKRQRVDGXXXXX-----XXXXXXXXXXXXXXSRSENGASAKDVTVDDQIAALERELKGADVSSSDAESSISSXXXXXXXXXXXXXXX------ERGRATKLVSPLEAEKIEPLPAHLLPRPGCGVHKDNKKKKKPSAAVGTTA---LPGPSQGLDSAVKELLANYEARSSERVPFYCRVCKFQGDSLEALEKHKEEPLHVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGKAHLEKLQSMMSRQGKWVPGGRGGPGSEGSLNGYSGRENGGDCWRGSGARGGWAVGRGRGNDGMRGGGNDGXXXXXX--PSRGRXXXXXXPGRGYENDR 353
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: C2H2-type (domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z659_9STRA)

HSP 1 Score: 70.5 bits (171), Expect = 2.770e-11
Identity = 30/49 (61.22%), Postives = 38/49 (77.55%), Query Frame = 3 
Query:  669 GLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLHVE 815
            GL+ AV EL+ NYEA+S+ER+P+YCRVCKFQG S+E L  H+   LH E
Sbjct:    2 GLEGAVNELLANYEAKSAERIPYYCRVCKFQGASVEDLAAHRQTQLHTE 50
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: C2h2 (zinc finger protein (Fragment) n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CRP9_NANGC)

HSP 1 Score: 62.4 bits (150), Expect = 1.780e-8
Identity = 28/47 (59.57%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  669 GLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLH 809
            GL   V EL+ NY+  SSER PFYCR+C+FQG SLE L  HK   LH
Sbjct:    2 GLAQTVAELLSNYQPASSERRPFYCRICRFQGQSLEDLAHHKKGELH 48
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: C2h2 (zinc finger n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TIZ9_9STRA)

HSP 1 Score: 62.4 bits (150), Expect = 6.180e-8
Identity = 28/47 (59.57%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  669 GLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLH 809
            GL   V EL+ NY+  SSER PFYCR+C+FQG SLE L  HK   LH
Sbjct:   33 GLAQTVAELLSNYQPASSERRPFYCRICRFQGQSLEDLAHHKKGELH 79
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: C2H2-type (domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D0V7_9STRA)

HSP 1 Score: 62.4 bits (150), Expect = 5.950e-7
Identity = 28/47 (59.57%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  669 GLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLH 809
            GL   V EL+ NY+  SSER PFYCR+C+FQG SLE L  HK   LH
Sbjct:  168 GLAQTVAELLSNYQPASSERRPFYCRICRFQGQSLEDLAHHKKGELH 214
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BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Match: Predicted (protein n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FRV3_PHATC)

HSP 1 Score: 61.6 bits (148), Expect = 9.720e-7
Identity = 37/107 (34.58%), Postives = 56/107 (52.34%), Query Frame = 3 
Query:  516 VSPLEAEKIEPLPAHLLP----RAGCGVHKDKXXXXXXXXXXXXXXXXXVPGPSQGLDSAVKELMENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLHVEAAK 824
            +S ++ ++IEPLP++LLP    R+  G+ +                   V   S GL++AVKE+++ Y  RSSER+PFYCRVC  Q    ++   HK    H  A +
Sbjct:   91 LSSVQKDRIEPLPSNLLPSNRKRSLKGIDRVNASERDDADQSNKKKSKLV---SSGLEAAVKEVLDGYVPRSSERLPFYCRVCAQQMSDEDSFTAHKETEFHTTAVE 194
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig8144.16299.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
C2H21.280e-9580.32zinc finger protein n=1 Tax=Ectocarpus siliculosus... [more]
C2H2-type4.880e-9571.27domain-containing protein n=1 Tax=Ectocarpus sp. C... [more]
C2H2-type2.770e-1161.22domain-containing protein (Fragment) n=1 Tax=Tribo... [more]
C2h21.780e-859.57zinc finger protein (Fragment) n=1 Tax=Nannochloro... [more]
C2h26.180e-859.57zinc finger n=1 Tax=Nannochloropsis gaditana TaxID... [more]
C2H2-type5.950e-759.57domain-containing protein n=1 Tax=Nannochloropsis ... [more]
Predicted9.720e-734.58protein n=2 Tax=Phaeodactylum tricornutum TaxID=28... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig8144contigE_fasciculatus_S2_contig8144:1673..5177 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score63.2
Seed ortholog evalue3e-07
Seed eggNOG ortholog2850.Phatr32507
Model size2050
Hectar predicted targeting categoryother localisation
Exons3
EggNOG free text desc.Zinc-finger double-stranded RNA-binding
EggNOG OGs2C7FU@1,2RRAS@2759,2XCKR@2836
Ec32 ortholog descriptionC2H2 zinc finger protein
Ec32 orthologEc-06_001270.1
Cds size1110
COG Functional cat.A
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842476.3124785-UTR-E_fasciculatus_S2_contig8144:1672..17671696842476.3124785-UTR-E_fasciculatus_S2_contig8144:1672..1767Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig8144 1673..1767 +
1696842476.3650534-UTR-E_fasciculatus_S2_contig8144:4332..51771696842476.3650534-UTR-E_fasciculatus_S2_contig8144:4332..5177Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig8144 4333..5177 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842476.332055-CDS-E_fasciculatus_S2_contig8144:1767..24411696842476.332055-CDS-E_fasciculatus_S2_contig8144:1767..2441Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8144 1768..2441 +
1696842476.3416548-CDS-E_fasciculatus_S2_contig8144:3301..34221696842476.3416548-CDS-E_fasciculatus_S2_contig8144:3301..3422Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8144 3302..3422 +
1696842476.3550584-CDS-E_fasciculatus_S2_contig8144:4017..43321696842476.3550584-CDS-E_fasciculatus_S2_contig8144:4017..4332Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8144 4018..4332 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig8144.16299.1prot_E_fasciculatus_S2_contig8144.16299.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig8144 1768..4332 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig8144.16299.1

>prot_E_fasciculatus_S2_contig8144.16299.1 ID=prot_E_fasciculatus_S2_contig8144.16299.1|Name=mRNA_E_fasciculatus_S2_contig8144.16299.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=370bp
MSRPPTMSQLLKRAGLAKRPRPAGRGVDATPSIANQSNKKRSRVDGGSRQ
HSSQEEEEAEQEEEEEERGRSRNENGASAKDVAVDDQIAALERELKGTDG
SSSDADSSSDAESSSSSDDDSASSKGSRGAGRKRGRVEKLVSPLEAEKIE
PLPAHLLPRAGCGVHKDKKKKKPRPKTGAGAGATAVPGPSQGLDSAVKEL
MENYEARSSERVPFYCRVCKFQGDSLEALEKHKTEPLHVEAAKKERKISK
CLLCKKQFTSPPQLQEHLAGKAHLEKLQSTMSRQGKWVPGGRGGSESEGS
SNGYSGRENGDGRWRGGGARGGGWAAGRGRGRDGGRGGRDGGGGRGRGDP
SRGRGGRGRGPGRGYERDR*
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mRNA from alignment at E_fasciculatus_S2_contig8144:1673..5177+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig8144.16299.1 ID=mRNA_E_fasciculatus_S2_contig8144.16299.1|Name=mRNA_E_fasciculatus_S2_contig8144.16299.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=3505bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8144:1673..5177+ (Ectocarpus fasciculatus EfasUO2)
CAGCAGGACAGGTCAGGTTGCTGCCTGTGCCGCACACTGCTCTGAGCAGG
CTTCTGCCACTCTTGAGCGGGGCTAGCCCCACACTACACAGCAAGATGTC
CCGACCTCCTACAATGTCGCAGCTGCTCAAGCGGGCGGGGCTGGCGAAAC
GCCCTCGGCCTGCAGGCCGCGGTGTAGACGCGACGCCGAGCATCGCAAAC
CAGTCCAACAAGAAGCGATCAAGGGTAGACGGCGGCAGCAGACAACACTC
GAGCCAGGAGGAGGAGGAGGCGGAGCAGGAGGAGGAGGAGGAGGAAAGAG
GGCGGAGTAGAAACGAGAACGGTGCTTCTGCCAAAGATGTCGCCGTGGAC
GACCAAATCGCGGCCCTTGAGCGCGAGCTCAAAGGCACCGACGGCAGCAG
TAGCGACGCCGATAGCAGTAGCGACGCCGAGAGCTCTAGTAGCAGCGACG
ATGATAGCGCCAGCAGCAAGGGCAGCAGAGGAGCCGGCCGAAAGCGCGGG
CGAGTGGAGAAGCTCGTGTCTCCTCTCGAAGCCGAAAAGATCGAGCCCCT
GCCCGCGCATCTCTTGCCGCGCGCGGGGTGCGGCGTGCACAAGGACAAGA
AGAAGAAGAAGCCTAGGCCGAAGACGGGTGCTGGTGCTGGTGCTACGGCG
GTGCCAGGACCTTCTCAAGGGCTGGATAGCGCCGTGAAGGAGCTCATGGA
AAACTATGAGGCCCGATCTTCCGAGCGGGTCCCGTTCTACTGCAGAGTGT
GCAAGTTCCAGGGAGATAGGTGTGTGGCTAGCTTGACTGCGGTCGGCACT
CAGCTTCATGCTGGAATCATGCGGTGATGCGTCTGCCATTGTTGTTACTG
CTGTTGGTGGCAGGATCGAGAGCGTACCCCCATGAACTAACTCGCCACCT
ATGAGCTCGGTATGCCCACAAGCAAGGCTTGCCGCCTTGCCGTGCTCGGC
GTATGCAGTTTCGATGTGTTTCGTCTCCTCTATCTTCTCCGAGAATAGCC
CTTGTTGTACAAGTCTCCACCTGGTGCATAGTAGTGCCTTCCCAGCGAAA
ACTCCTCCATCTACTCGCAACCTGATCCAAGCCTACCTGTGTGCTTGCTG
GCTACCCCAACCGCACCCATAAGTGACCACAGACCTCACTACGTGTGTCT
CCAAACCAAGGACACGAGCGCTGTAGGTTCTTCGTTCTAGGCGAGGATGG
TAACTTCGACTTCCAATTTGGTAGCATGTGTTTCGGAAGCATTTGCTTGC
GCAAGCTTACGCAGTGTTCGACAGGGCAGAGCATTCCACAAAGTGTGTTG
CATGCAGTTGGCTCCATGTGCAGGCTAATTATATCAAGCGTTCGATCGCT
GCTGTGCGTTGTTGTAGCCAGACAGCTCCACTAGAGACCACGATGATCGG
TATCACGACCTCACCACCGCTGGCGTGATGCATACGTACCTCTCTGTGCT
ATTTACAGTGGCAGTGATGGATAGCATAGCATTGAATCCGTATTTCTGTC
TTACCTCTCCCGGTTGCCGATGACCTCCAACCCATATGTTTGTCCCTCTA
ACCTACGCTCCTTTTAACCGTTGCGTATGTGGCGTATCTCCACCATACGC
GGTATATTCTGGCCGGATGAACGGATCAGCTTGGAGGCGCTTGAGAAGCA
CAAAACAGAACCGCTGCACGTGGAGGCCGCCAAGAAGGAGCGGAAGATTA
GCAAGTGCTTGCTCTGCAAGAAGCAGTTCACCAGTCCACCCCAGTTGCAG
GTACTGGTCATGTCCACCCCGGCGTTGTTCGTCGTAACCCTACATGGCGC
TTTGCTGCCTTGCCGCTATATGCTGGCTTACCGCCTCGTAGAAGTCGTTG
AAATGCATTGTTGGGGATGCGCTGTCCTGTGCGGAACGGTCGTGCTCTAC
AAGTGACGCGTTCTTAGATGATGTTCGAGGAGTCGTTTGCGGGGAGATAG
TTTCAGAGCTGTCGGCGCGCGCCGTGCTGTGTTTTTTTTGTTTCTCACGG
CACGTGCGTGGGTGCTGTTGTTAGTACCAGCGCCGGACCTTTTCTCTCCT
ATTTTACTGTACATTGCAGTCTATTTTGGTTGATGCTGCTACGCTGCTGT
TACGAGGCCGCACGTCGTTTTCATCCCACGTCGGCCCTTCTGAACGGCGC
ATGGGCTATATCTACTACTTATGTTGCACTTCGGCACTCAACGCGCGGCG
TGCAACGTGAACCTTCCTTTCCGTTCGCAACAATTGTCCGTCTCCCTAGC
TCCCTGTGTGACCTCCGTGGGTCGCCAACGTTAACCTTGCAAACACGGTC
CGATGCTTGCCAACCTTAACCCAACCGTGCAAATGATGTTGACAGGAACA
CCTGGCAGGGAAGGCTCACCTGGAAAAGCTCCAGTCGACCATGTCGCGCC
AGGGGAAGTGGGTTCCCGGTGGTCGGGGAGGCTCCGAATCAGAGGGATCG
TCGAACGGATATTCTGGTCGAGAAAACGGGGACGGTCGCTGGCGAGGGGG
CGGTGCACGTGGCGGCGGATGGGCGGCGGGTCGGGGGAGAGGAAGGGATG
GAGGGAGAGGAGGGAGGGATGGCGGCGGTGGCCGGGGGCGGGGAGATCCG
AGTCGCGGGCGGGGCGGGCGGGGGAGGGGACCGGGAAGAGGATACGAAAG
AGATCGATGACGACGGTTGTTCTGCTCCGTGGTGTAGTCAGTCATCGGTA
TGTGTGCTGAGTGCACATACAAGTTCGTAGGAGTGTGTGCAGAGTGCGCG
TACACTTCAATGAGAACTCGCGACCTCCATCTTCCCCACGCAATGCATTC
CTACTGTCTGTAGGAGTGGGAAGCGGTGGGTCCTCAAGCGGATGGAGCCT
TAGTCCTCCTTTGTTTTGATAGAGATGAACGGGGGACTCGGTATAACACA
AGGTGGTCAACGAGGTCGTCAGCGAAATTGTACTGCGGTGTATTTGTCAA
GGGCGACTCTCTGTTCTTTGCGTAGAGGGCGGCAGAGCGTAGTAGGTTGG
AACGACTCGCACACTCTGCTGCTGTAGGCCATTACACATACACATGCGAA
GCACCAATATCGCGTTGACGGCGTTGGTCTTCTGGCTTGAGACTCCCGAT
GAACGAATTACGCGTGTCCGATCTCGTCTCCGTGGAATTGGCAGATGATG
ACCACAAAATTGCTGTTGTCAGTTCGTTGTCCCCCAGGTTGTAGGCAAAC
TACAGGTGTGTGGTTGTCTTCAACAGCTGATTTTGTAGCTCGATTGCTCT
ACAAGTACAACAAAGAGGCATCCCCAATGCCGAAGAATCTAGGTTTGGAA
CGCTCTCCCAGAGAGAGAGAGACAGAGAGAGAGAGAGAGCTGTTCTTGCA
CCTGTCAGTCGGGGTTCGAAAGCACCCTCTCTTGTTCGTGGAGCATTCAA
CCGTCGATCGCACACGTACCTGTATGTTGGGGCCCGGACTCGGTACTACA
AAACGGCTGGAGCACGTAGGGTTGTGCTAGTTTGGGAGCTGCCAACAGAG
AGTCC
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig8144:1673..5177+

>mRNA_E_fasciculatus_S2_contig8144.16299.1 ID=mRNA_E_fasciculatus_S2_contig8144.16299.1|Name=mRNA_E_fasciculatus_S2_contig8144.16299.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=1110bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8144:1673..5177+ (Ectocarpus fasciculatus EfasUO2)
ATGTCCCGACCTCCTACAATGTCGCAGCTGCTCAAGCGGGCGGGGCTGGC
GAAACGCCCTCGGCCTGCAGGCCGCGGTGTAGACGCGACGCCGAGCATCG
CAAACCAGTCCAACAAGAAGCGATCAAGGGTAGACGGCGGCAGCAGACAA
CACTCGAGCCAGGAGGAGGAGGAGGCGGAGCAGGAGGAGGAGGAGGAGGA
AAGAGGGCGGAGTAGAAACGAGAACGGTGCTTCTGCCAAAGATGTCGCCG
TGGACGACCAAATCGCGGCCCTTGAGCGCGAGCTCAAAGGCACCGACGGC
AGCAGTAGCGACGCCGATAGCAGTAGCGACGCCGAGAGCTCTAGTAGCAG
CGACGATGATAGCGCCAGCAGCAAGGGCAGCAGAGGAGCCGGCCGAAAGC
GCGGGCGAGTGGAGAAGCTCGTGTCTCCTCTCGAAGCCGAAAAGATCGAG
CCCCTGCCCGCGCATCTCTTGCCGCGCGCGGGGTGCGGCGTGCACAAGGA
CAAGAAGAAGAAGAAGCCTAGGCCGAAGACGGGTGCTGGTGCTGGTGCTA
CGGCGGTGCCAGGACCTTCTCAAGGGCTGGATAGCGCCGTGAAGGAGCTC
ATGGAAAACTATGAGGCCCGATCTTCCGAGCGGGTCCCGTTCTACTGCAG
AGTGTGCAAGTTCCAGGGAGATAGCTTGGAGGCGCTTGAGAAGCACAAAA
CAGAACCGCTGCACGTGGAGGCCGCCAAGAAGGAGCGGAAGATTAGCAAG
TGCTTGCTCTGCAAGAAGCAGTTCACCAGTCCACCCCAGTTGCAGGAACA
CCTGGCAGGGAAGGCTCACCTGGAAAAGCTCCAGTCGACCATGTCGCGCC
AGGGGAAGTGGGTTCCCGGTGGTCGGGGAGGCTCCGAATCAGAGGGATCG
TCGAACGGATATTCTGGTCGAGAAAACGGGGACGGTCGCTGGCGAGGGGG
CGGTGCACGTGGCGGCGGATGGGCGGCGGGTCGGGGGAGAGGAAGGGATG
GAGGGAGAGGAGGGAGGGATGGCGGCGGTGGCCGGGGGCGGGGAGATCCG
AGTCGCGGGCGGGGCGGGCGGGGGAGGGGACCGGGAAGAGGATACGAAAG
AGATCGATGA
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