mRNA_E_fasciculatus_S2_contig743.15607.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig743.15607.1
Unique NamemRNA_E_fasciculatus_S2_contig743.15607.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LGV4_ECTSI)

HSP 1 Score: 285 bits (728), Expect = 1.370e-93
Identity = 163/196 (83.16%), Postives = 171/196 (87.24%), Query Frame = 1 
Query:  202 VPPLSATTDDSGENGVITGSNQLEGWKRNTVGAAADAPTRSNVMGRRR-GDGSNGGDPAAAA-GNTEPAPFAPRGGDGGVADGGNSADGATAWRVEAAATSAELLLSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLASEAIHTAEA-GAAPAPAAGQ 780
            +P L AT DDSG++GVI GSNQLEGWKRNTVG AADAPTRSNVMGRRR GDGSNG DPAAAA GNTEPA  +P     G A GG SAD ATAWRVEAAAT+AELL+SFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLASEA+H AEA  AAP  A G+
Sbjct:    1 MPSLPATPDDSGDSGVIAGSNQLEGWKRNTVGTAADAPTRSNVMGRRRPGDGSNGCDPAAAAAGNTEPASLSPH--HRGDAYGG-SADDATAWRVEAAATTAELLVSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLASEAMHAAEARAAAPVGAQGR 193
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: 23S (rRNA (Cytosine1962-C5)-methyltransferase n=1 Tax=Pseudomonas oryzae TaxID=1392877 RepID=A0A1H1Z0I6_9PSED)

HSP 1 Score: 81.3 bits (199), Expect = 8.770e-14
Identity = 45/104 (43.27%), Postives = 60/104 (57.69%), Query Frame = 1 
Query:  514 SFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLASEAIHTAEAGAAPAPAAGQAAKEAVAFLPVWNDR 825
            + + ALA R+PL+ +L E+ TDCYRL+HG+ EG PGLTVDRYG  +LVQ+F  P      EA+ E+    +EA+                   A A LPV+NDR
Sbjct:    7 ALRAALARRQPLLAELHEQGTDCYRLFHGSQEGAPGLTVDRYGPQLLVQSFHQP---LEREALLELFATCAEAL-------------------ATALLPVYNDR 88
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: Class (I SAM-dependent rRNA methyltransferase n=3 Tax=Zoogloea TaxID=349 RepID=A0A848G2P1_9RHOO)

HSP 1 Score: 78.2 bits (191), Expect = 1.030e-12
Identity = 39/67 (58.21%), Postives = 46/67 (68.66%), Query Frame = 1 
Query:  505 LLLSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVE 705
            L+ S   A  ARE LM +LL E TD YRL+HG VEG PGLTVDRYG++VLVQ+F  P +D     VE
Sbjct:    5 LVTSLNAAFQAREALMARLLAEGTDAYRLFHGTVEGRPGLTVDRYGSLVLVQSFHSPLSDDEESEVE 71
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: Class (I SAM-dependent rRNA methyltransferase n=8 Tax=Proteobacteria TaxID=1224 RepID=A0A3S0DVE1_9RHOO)

HSP 1 Score: 77.4 bits (189), Expect = 1.900e-12
Identity = 47/104 (45.19%), Postives = 60/104 (57.69%), Query Frame = 1 
Query:  517 FKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLASEAIHTAEAGAAPAPAAGQAAKEAVAFLP-VWNDR 825
              TALAAR PL+ +L  E+TD +RL+HG VEG PG+TVDRYG V+LVQTF  P    +AE + E+     EA +                 +A+  LP VWNDR
Sbjct:    9 LSTALAARAPLIARLAAEDTDAWRLFHGTVEGAPGITVDRYGAVLLVQTFHRP---LTAEQLAEL-----EAFYA----------------QALPGLPLVWNDR 88
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: Class (I SAM-dependent rRNA methyltransferase n=1 Tax=Rhodocyclaceae bacterium TaxID=1898103 RepID=A0A838LSP6_9RHOO)

HSP 1 Score: 77.0 bits (188), Expect = 2.580e-12
Identity = 39/68 (57.35%), Postives = 49/68 (72.06%), Query Frame = 1 
Query:  496 SAELLLSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDP--PADFSA 693
            SA L+ S   AL +R  L+R+L  EETD YRL+HG+VEG PGLTVDRYG ++L+QTF  P  PA  +A
Sbjct:    2 SANLIQSLAAALDSRAALLRQLHTEETDAYRLFHGSVEGQPGLTVDRYGELLLIQTFHSPLPPAQLAA 69
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: 23S (rRNA (Cytosine1962-C5)-methyltransferase n=1 Tax=Amphritea japonica ATCC BAA-1530 TaxID=1278309 RepID=A0A7R6PAV1_9GAMM)

HSP 1 Score: 76.3 bits (186), Expect = 4.540e-12
Identity = 37/69 (53.62%), Postives = 47/69 (68.12%), Query Frame = 1 
Query:  505 LLLSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEI 711
            +L   + ALA R  L+  L EE TDCYRLYHG+ EGC GLTVDRYG  +L+QTF  P +   AEA+ ++
Sbjct:    1 MLNLIQDALARRSELLDALHEENTDCYRLYHGSNEGCSGLTVDRYGPQLLIQTFHQPISVEEAEAIHKL 69
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: Class (I SAM-dependent rRNA methyltransferase n=1 Tax=Amphritea balenae TaxID=452629 RepID=A0A3P1SVB3_9GAMM)

HSP 1 Score: 76.3 bits (186), Expect = 4.540e-12
Identity = 35/73 (47.95%), Postives = 49/73 (67.12%), Query Frame = 1 
Query:  505 LLLSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLA 723
            ++   + ALA R  L+ +L +E TDCYRL+HG  EGC GLTVDRYG  +LVQTF +P     AE+++++   A
Sbjct:    1 MISQLQQALANRTGLLEQLHDENTDCYRLFHGTNEGCAGLTVDRYGPQLLVQTFHEPIDQTEAESIQQVTEQA 73
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: SAM-dependent (methyltransferase n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A2E7M9I0_9DELT)

HSP 1 Score: 75.1 bits (183), Expect = 1.160e-11
Identity = 35/65 (53.85%), Postives = 46/65 (70.77%), Query Frame = 1 
Query:  523 TALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICR 717
            TA+  R  L+ +L +E+TDCYRL+HGAVEG PGL VDRYG V+LVQ++ D P     EA+  + R
Sbjct:   13 TAIDRRRALLERLHQEQTDCYRLFHGAVEGRPGLAVDRYGPVLLVQSWGDGPPQDEVEAMAAVAR 77
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: Methyltrans_SAM (domain-containing protein n=1 Tax=Elusimicrobia bacterium GWA2_62_23 TaxID=1797924 RepID=A0A1F9RGF4_9BACT)

HSP 1 Score: 75.1 bits (183), Expect = 1.190e-11
Identity = 38/70 (54.29%), Postives = 45/70 (64.29%), Query Frame = 1 
Query:  514 SFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLA 723
            SF  AL  R PL+ +L  E TDC RL+HG  EG PGLTVDRYG ++L QTFRD  +    EA+E   R A
Sbjct:    9 SFSQALDRRRPLLERLEAEGTDCCRLFHGIAEGLPGLTVDRYGPLLLAQTFRDCLSPSETEALEATLRKA 78
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BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Match: class (I SAM-dependent methyltransferase n=1 Tax=Zoogloea sp. 1C4 TaxID=2570190 RepID=UPI001292363E)

HSP 1 Score: 75.1 bits (183), Expect = 1.190e-11
Identity = 35/69 (50.72%), Postives = 48/69 (69.57%), Query Frame = 1 
Query:  499 AELLLSFKTALAAREPLMRKLLEEETDCYRLYHGAVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVE 705
            A L  +   AL AR  L+ +L  + TD +RL+HG VEG PGLTVDRYG+++LVQ+F DP +  + EA+E
Sbjct:    3 ANLTAALARALEARRDLLARLASDHTDAFRLFHGTVEGHPGLTVDRYGSIILVQSFHDPLSPAALEALE 71
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig743.15607.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Uncharacterized1.370e-9383.16protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
23S8.770e-1443.27rRNA (Cytosine1962-C5)-methyltransferase n=1 Tax=P... [more]
Class1.030e-1258.21I SAM-dependent rRNA methyltransferase n=3 Tax=Zoo... [more]
Class1.900e-1245.19I SAM-dependent rRNA methyltransferase n=8 Tax=Pro... [more]
Class2.580e-1257.35I SAM-dependent rRNA methyltransferase n=1 Tax=Rho... [more]
23S4.540e-1253.62rRNA (Cytosine1962-C5)-methyltransferase n=1 Tax=A... [more]
Class4.540e-1247.95I SAM-dependent rRNA methyltransferase n=1 Tax=Amp... [more]
SAM-dependent1.160e-1153.85methyltransferase n=1 Tax=Deltaproteobacteria bact... [more]
Methyltrans_SAM1.190e-1154.29domain-containing protein n=1 Tax=Elusimicrobia ba... [more]
class1.190e-1150.72I SAM-dependent methyltransferase n=1 Tax=Zoogloea... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig743contigE_fasciculatus_S2_contig743:24810..27956 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeBacteria
Stop0
Start0
Seed ortholog score75.9
Seed ortholog evalue3.8e-11
Seed eggNOG ortholog1278309.KB907099_gene3091
Model size942
KEGG koko:K06969
Hectar predicted targeting categorysignal peptide
Exons4
EggNOG free text desc.S-adenosylmethionine-dependent methyltransferase
EggNOG OGs1R6GY@1224,1RZ4H@1236,1XNXK@135619,COG1092@1,COG1092@2
Ec32 ortholog descriptionS-adenosyl-L-methionine-dependent methyltransferase
Ec32 orthologEc-28_001320.1
EC2.1.1.191
Cds size942
COG Functional cat.J
Best tax levelOceanospirillales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03009
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842406.2138634-CDS-E_fasciculatus_S2_contig743:24809..253311696842406.2138634-CDS-E_fasciculatus_S2_contig743:24809..25331Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig743 24810..25331 +
1696842406.2256699-CDS-E_fasciculatus_S2_contig743:25765..258881696842406.2256699-CDS-E_fasciculatus_S2_contig743:25765..25888Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig743 25766..25888 +
1696842406.2350507-CDS-E_fasciculatus_S2_contig743:26693..268291696842406.2350507-CDS-E_fasciculatus_S2_contig743:26693..26829Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig743 26694..26829 +
1696842406.2452445-CDS-E_fasciculatus_S2_contig743:27795..279561696842406.2452445-CDS-E_fasciculatus_S2_contig743:27795..27956Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig743 27796..27956 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig743.15607.1prot_E_fasciculatus_S2_contig743.15607.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig743 24810..27956 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig743.15607.1

>prot_E_fasciculatus_S2_contig743.15607.1 ID=prot_E_fasciculatus_S2_contig743.15607.1|Name=mRNA_E_fasciculatus_S2_contig743.15607.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=314bp
GRREQPRAIRPVVMMARRRDGVCRHAVHALLLLLLGLPVCQPFASPNVNL
RLRSCTVTGRAPNHPVGVPPLSATTDDSGENGVITGSNQLEGWKRNTVGA
AADAPTRSNVMGRRRGDGSNGGDPAAAAGNTEPAPFAPRGGDGGVADGGN
SADGATAWRVEAAATSAELLLSFKTALAAREPLMRKLLEEETDCYRLYHG
AVEGCPGLTVDRYGTVVLVQTFRDPPADFSAEAVEEICRLASEAIHTAEA
GAAPAPAAGQAAKEAVAFLPVWNDRRKRERRGRRGGGRDYDDVPPPHRLS
SDHEPSPEALGVKV
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mRNA from alignment at E_fasciculatus_S2_contig743:24810..27956+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig743.15607.1 ID=mRNA_E_fasciculatus_S2_contig743.15607.1|Name=mRNA_E_fasciculatus_S2_contig743.15607.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=3147bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig743:24810..27956+ (Ectocarpus fasciculatus EfasUO2)
GGGCGAAGAGAACAACCGAGGGCCATCCGACCTGTGGTGATGATGGCGCG
TCGAAGGGACGGCGTGTGTCGTCACGCAGTGCACGCCCTACTACTGCTAC
TCCTTGGTTTGCCGGTATGTCAGCCCTTTGCCTCGCCGAACGTCAACCTG
CGGCTGAGATCATGCACCGTGACAGGCAGGGCGCCGAACCACCCCGTTGG
CGTGCCGCCGCTGTCAGCAACGACCGACGACAGTGGAGAGAACGGCGTAA
TCACGGGGTCGAACCAGCTGGAAGGCTGGAAGAGGAACACCGTTGGCGCT
GCTGCGGATGCACCCACGAGGTCGAACGTTATGGGGCGCCGTCGGGGGGA
CGGGAGCAACGGCGGTGATCCTGCTGCTGCGGCCGGCAACACGGAGCCGG
CACCATTTGCCCCTCGTGGTGGTGATGGGGGGGTTGCGGACGGTGGTAAT
AGTGCGGACGGTGCAACGGCATGGCGGGTGGAAGCAGCCGCGACAAGTGC
GGAGCTGCTGCTATCCTTCAAGGTTAGCAAAGGGACAAAGGGATGGCCGT
CGCGGTCTCGTTTTGTTACATTGCACCGCCGTTGAATTTGTGACTCTTGC
GCCCTTGGCTTGCATACACTTTTATACAATACATACTGCTGTCGCGACAC
GATCGGGAACTTCTCCTCTTGCGGGTACACCGTACTGAGCTGTGCTTGCT
GCAGTATTCCGTTGTATTGTCACGTCTTCGCGAACGACTTGCAAGGCCCA
GGAGTAGTGGCTGAGTGAGACTTCGTCGTTGCACTTGAATGTTTTCCTCC
CAAAATGTAGCCTATGTACAGGTGTTCGATACATCAAGGAACAACCACAA
CGGCATGTTTGATGTCTCATCTGTTCGTTCGCCCCCACCCCTTCCCCCAC
CCCCTTCGTGGATCACATTCGCTATGCGGCAAAACTCTTCCCCGTCAATC
GAACAGACGGCGCTGGCGGCAAGGGAGCCACTGATGCGGAAGCTACTAGA
GGAGGAAACGGACTGTTACCGGCTGTATCACGGTGCCGTGGAAGGCTGCC
CCGGCCTTACCGTAGACCGTTACGGCACGGTACGTTTACCACAAGAATGT
CTCCCGTTCCAGATTTCTCTCCATGTTGGCCAACACACACTACCAGACCC
CACTTTTACAGCAGAGCCGACTCCCCAGGCGAACGACGACAGCGTAGACA
GATGGAGGAAACGGCAGCCGACTTAACCGCGAACGGCTGCCGTCGTCCTT
ACGGTCTATCTGTGCGTGGGCAGATACTTTGAAGGACGGGTGTTACTCAT
TTTCTCGTCTGCTTATGCCCTTGTTGTGAGTATCACGCGCGTGAGGCAAG
GGTCATACTTCGGAGCAAAGCAACACGATGGGCGTTGGTCGCTCTGCAGC
ACGTACGGTAGTATGCATACAATCCTTCTTGCACGTCCTAGGGTGCCAGA
ACCAGAACTATACAAATGTTGCCCTTCATTTGTCGAAGTCTCGTGCAAGA
ACAACGACGGGGACGACGACAACAACAGCCGCGGCAACACCAAGCGATGA
CCCATCCGTACACCAAAAAACAAGAGTAATTTTCCTTTCTTTTATTGTTT
GGTTGTTGTTGTCTCGGCCCCTCCTTGTTCTCGATGCTCTTTGTTCTTGT
TCTTGACGGGCACGCAGCAAGTGAAAACAAAGAAAAACATGTCGTGAGTG
TGGTTAACACTTCCCTCAGCTTAAGCGTGTTTCGCCTCTGCCGCATGAGA
AGTGTAGTTCACGTCTCACCACGGCAATGGGGGACTGGTATCGCCATTAT
TCAGGACGAAACACACGATAACACCGCATGGCTGCGACGCTTCGCGGACA
CACTTCACCCGCGATAAAATACAACTACATGTAGGTTGTCCTGGTGCAAA
CCTTTCGGGACCCGCCGGCCGACTTCAGCGCTGAGGCGGTGGAGGAGATT
TGCAGACTGGCGTCCGAAGCGATACACACCGCGGAGGCGGGAGCAGCACC
AGCACCAGCAGCAGGGCAAGGTGAGGACCGGGAGCGTTACTTTGCTTCTC
TTCGAGGAGGCATCTTCGGATAGTCTTGTTGTTATGTTGTTGTTGTTGCT
GTTGCTACTGCTGCTGGTTGTGGTGGTGGTGGGTCTGGTGCCGCACTTGC
CATTTCTCTTGGCTGCTTCCGCGGCCGTTTTGTTCTAGACGAAGTACATG
TTTTGTTGGTTTTAGCGCGGAAACTGCTCCATGTATTGTGTGCACAGCTA
GATTTTCCCTAACAACATCGAGGGAGCCGGGGTGCGTTCGCCGTTCCAGG
GACATACCACCAGAGAAACCAAAAGATTATATATCACCTCTCGGACGCGT
AGATACCTCTGTTGGCGTTTACAAGACAACGACGTCCGCCAACATGACTC
GGAAGGAAGGCAGCGCAGGTGACCATTCTTGGAATCGGCGTGGTCTCTTG
ATCATGGGGGTAGGTGCGCCCAAGCACCTGGTTCGTTGATGAAAAGCAGA
AAAATCTTGAAAAAAAATGCGTGCCCCCACCCCCCACCCCGTTTTCCCCA
CGGGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCCCCCC
CTNNNNNNNNNNAAAAACCAAAAAAACCTTAAAAAAAATTGCTTCCCCCC
ACCCCCACCCCTTTTCCCCCCCCGGCCCCCCCCCCCCCCTGCCCTACCCT
GTCCCTACCTGTCGCCCCCGAAGCGTCCTTCGGTGCAAGAGTCGATAGCT
TTTTTTAACAAACACTTTGTAATCACCCGTTCGAGAAAAAACCCAGTCCA
AGGGATGTGCTCAAGTCTATTCAGCAGCAGGGAGTGGGGAGAGAAACCCG
CTGAATGCTGAGCTGTTTTTTTAGTCTGTTTGAGCCTATACACTGAAACC
GATACTGCTGTGTACCGAGCTTTGATCTCGTGCCGTCCTGTACCGATGCG
TTTGAAAAAAACGGAAAACGATGACGACAATGCCAGCGGCCAAGGAAGCA
GTCGCGTTTCTTCCCGTCTGGAACGATCGGCGCAAGAGAGAACGCCGCGG
CAGGAGGGGGGGTGGAAGGGACTACGACGACGTTCCCCCGCCGCACAGGC
TAAGCTCGGACCACGAGCCCTCGCCGGAAGCACTAGGAGTCAAGGTA
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig743:24810..27956+

>mRNA_E_fasciculatus_S2_contig743.15607.1 ID=mRNA_E_fasciculatus_S2_contig743.15607.1|Name=mRNA_E_fasciculatus_S2_contig743.15607.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=942bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig743:24810..27956+ (Ectocarpus fasciculatus EfasUO2)
GGGCGAAGAGAACAACCGAGGGCCATCCGACCTGTGGTGATGATGGCGCG
TCGAAGGGACGGCGTGTGTCGTCACGCAGTGCACGCCCTACTACTGCTAC
TCCTTGGTTTGCCGGTATGTCAGCCCTTTGCCTCGCCGAACGTCAACCTG
CGGCTGAGATCATGCACCGTGACAGGCAGGGCGCCGAACCACCCCGTTGG
CGTGCCGCCGCTGTCAGCAACGACCGACGACAGTGGAGAGAACGGCGTAA
TCACGGGGTCGAACCAGCTGGAAGGCTGGAAGAGGAACACCGTTGGCGCT
GCTGCGGATGCACCCACGAGGTCGAACGTTATGGGGCGCCGTCGGGGGGA
CGGGAGCAACGGCGGTGATCCTGCTGCTGCGGCCGGCAACACGGAGCCGG
CACCATTTGCCCCTCGTGGTGGTGATGGGGGGGTTGCGGACGGTGGTAAT
AGTGCGGACGGTGCAACGGCATGGCGGGTGGAAGCAGCCGCGACAAGTGC
GGAGCTGCTGCTATCCTTCAAGACGGCGCTGGCGGCAAGGGAGCCACTGA
TGCGGAAGCTACTAGAGGAGGAAACGGACTGTTACCGGCTGTATCACGGT
GCCGTGGAAGGCTGCCCCGGCCTTACCGTAGACCGTTACGGCACGGTTGT
CCTGGTGCAAACCTTTCGGGACCCGCCGGCCGACTTCAGCGCTGAGGCGG
TGGAGGAGATTTGCAGACTGGCGTCCGAAGCGATACACACCGCGGAGGCG
GGAGCAGCACCAGCACCAGCAGCAGGGCAAGCGGCCAAGGAAGCAGTCGC
GTTTCTTCCCGTCTGGAACGATCGGCGCAAGAGAGAACGCCGCGGCAGGA
GGGGGGGTGGAAGGGACTACGACGACGTTCCCCCGCCGCACAGGCTAAGC
TCGGACCACGAGCCCTCGCCGGAAGCACTAGGAGTCAAGGTA
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