mRNA_E-fasciculatus_F_contig1067.532.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: D8LE29_ECTSI (Thiaminase II n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE29_ECTSI)

HSP 1 Score: 889 bits (2297), Expect = 8.430e-315
Identity = 476/608 (78.29%), Postives = 495/608 (81.41%), Query Frame = 2

Query:  362 MDGMRKDVGKGFTLPGSLWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSGQCVYRASQWAEIDVFLFGGE 2185
            MDGMRKDVGKG TLPGSLWQDCQLEAMRSLHHPWTLGLATG+TSLASFK+YVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN                                             ELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTE A+LEQSVGAE GGRQGMS QPGGQDAGGRGRF GVH++LALLCVDFDDTLTEGDTISLLVETAKAQRETPED+VD+GREWGLLTRTFL +W ETIEDSLSTKLS+T CTTNG+GAVDREGLE+MLRKL VVDLDSVSRVSDSK                                                   DVI+RVLSETLLDSHSS SSTTGID+GG +WAGETASPRL+GDCPASVSSNDLVFD+HGVSTGEI VKIPGSFGKH+RFLDLAA +RES ASSESSPRLMT+YVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMD TGELPR  APSGSGQCVYRASQWAEIDVFLFGGE
Sbjct:    1 MDGMRKDVGKGSTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN---------------------------------------------ELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGRQGMSAQPGGQDAGGRGRFRGVHERLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDRVDLGREWGLLTRTFLGKWSETIEDSLSTKLSTT-CTTNGSGAVDREGLELMLRKLGVVDLDSVSRVSDSK---------------------------------------------------DVISRVLSETLLDSHSSSSSTTGIDAGGCVWAGETASPRLEGDCPASVSSNDLVFDEHGVSTGEILVKIPGSFGKHQRFLDLAATARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTGELPRTGAPSGSGQCVYRASQWAEIDVFLFGGE 511          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A6H5J5C1_9PHAE (TENA_THI-4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J5C1_9PHAE)

HSP 1 Score: 790 bits (2040), Expect = 2.300e-274
Identity = 449/668 (67.22%), Postives = 482/668 (72.16%), Query Frame = 2

Query:  371 MRKDVGKGFTLPGSLWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN---------------TP----------------PPVKATAEYVDFLMAV--ASDPTSSVAQVLAAMVPCMRLYA-------------------------------FLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSGQCVYRASQWAEIDVFLFGG 2182
            MR DVG+ FTLPGSLWQDCQLEAMRSLHHPWTLGLATG+TSLASFK+YVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN               TP                  VK  +++   L  +   +      A+ L       R +A                               F+ +ELR A PNWTES YAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFAL RKAMELEFAFFDAQLDG TE A+LEQSVGAE GGRQGMSVQPGG+DAGGRGRF GVH++LALLCVDFDDTLTEGDT SLLVETAKAQRETPED+VD+GREW LLTRTFLS+W ETIEDSLSTKLS+TACTTNG+GAVDREGLE+MLRKLEVVDLDS SRVSDSKV                          +AR I R  ++              ++ R                           GETASPRL+GDCPASVSSNDLVFD+HGVSTGEIAVKIPGSFGKH+RFLDLAAM+RES ASSESSPRLMT+YVGDSVTDLLAMLDADVGIVVGNSGSFE+VARAFGIDI PLASVYEAMD  GELP   AP GSGQ VYRASQW EIDVFLFGG
Sbjct:    1 MRMDVGERFTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANVRAKTTGVKLQIGSCTPLNFGLTGVWNVVHCRFASVKLHSDWAHLLYPINQGNKQMQVSAKELGERAMKRRSFAGLYLASLQIGLYRRQFHQCNVMTFADVLSSLFVAQELRDALPNWTESPYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALCRKAMELEFAFFDAQLDGVTEAAMLEQSVGAEAGGRQGMSVQPGGKDAGGRGRFRGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSETIEDSLSTKLSTTACTTNGSGAVDREGLELMLRKLEVVDLDSASRVSDSKVRE------------------------LAREIRRYFLAAI------------IVQRE-------------------------PGETASPRLEGDCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFLDLAAMARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFERVARAFGIDILPLASVYEAMDGRGELPTTGAPLGSGQRVYRASQWTEIDVFLFGG 607          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A2V0PBE0_9CHLO (Thiamine phosphate synthase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0PBE0_9CHLO)

HSP 1 Score: 264 bits (675), Expect = 1.860e-69
Identity = 200/607 (32.95%), Postives = 281/607 (46.29%), Query Frame = 2

Query:  392 GFTLPGS----LWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDE--EDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETA---KAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHS-SLSSTTGIDSGGRIWAGETASP-RLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSGQCVYRASQWAEIDVFLFG 2179
            G  LPG     LW +CQLEA R+L HP+ L L  G     +F+ YVAQDA+FL  FA+AYA AL K  D   E       L+  V  EL LH SY+  WG+++        A   Y DFLMA A DP ++VA+VLA+MVPC RLY FLG  L  A P    + YA+WI  YSS ++       E+LLD+ +  A Y  L +LY++AM+LE AFF A                            PG   A          + + +L  DFDDT TE DTI  L+  A     +R  PE+   +      +    +  +       L+  L   A      G  D  GL   + +L   D+   +   +S +L GL+RD+V A+          ++PG    ++ AL +   THVVS+NWS++ +A  L     D+     + + G ++   + AG    P RL      ++ +N+L     G+STG +   +  +  K R F  L A     A           ++VGDS TD+L +L AD GIVVG++    +     G  + PL +        G     AA  G  + VY A+ W EI  FLFG
Sbjct:    9 GLPLPGGVSERLWGECQLEAFRALQHPFVLALGLGTLPRDTFRHYVAQDAHFLHFFARAYAAALDKCADANAEAHVVLQQLLMGVHMELRLHGSYARTWGVELDALDHVDPACQAYTDFLMATAEDPETTVAEVLASMVPCSRLYGFLGCALARAHPRQRGNPYADWISMYSSFEYLVHPMQKEALLDRLAGDAPYDRLRSLYKRAMQLEVAFFSAA---------------------------PGIPAA----------RHVGMLVCDFDDTCTEKDTIGTLLSAAIDAAVKRAGPEEXXXLRARLDAVVARLVENYSSKHAALLAEILPEGASAAAAEGGFDEAGLRAFVERLSDFDVQMNAVAVESGILAGLSRDDVAAAARAVA-----LKPGCREALAAALGAELPTHVVSVNWSREFVAAALGLPAADAADWGAAGSDGDEAAAAVIAGAAPPPGRL------AIHANELARGADGLSTGALRRAVQCARDKGRLFAALQA-----ARGKGGXXXXAAVFVGDSATDVLPLLAADYGIVVGSNKLLRRALARHGASLVPLCAAPLTAPARGG--GGAASDGVARVVYEAASWDEIHAFLFG 560          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A383WBI9_TETOB (Thiamine phosphate synthase n=2 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WBI9_TETOB)

HSP 1 Score: 252 bits (644), Expect = 3.110e-65
Identity = 196/629 (31.16%), Postives = 276/629 (43.88%), Query Frame = 2

Query:  380 DVGKGFTLPGSL----WQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVL----EELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTK----LSSTACTTNGAGAV-------------DREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLM---TIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASV-YEAMDKTGELPRRAAPSGSGQCVYRASQWAEIDVFLFG 2179
            D+  G  +PG L    W D Q EAM +LHHP+   LA G    A F+ YV QD +FL  FA+AYA A+ K      + A  +++  +L     EL LH SY+AKWG+ V     P  AT++Y DFLM VA+DP +SVA+VLAAM+PC RLY FLG +L  A       AY+EW+ TYS  ++  +  + E L DK +A   Y  L  LY +AM+LE  FF AQ +G                                        + + LL  DFDDT+TE DTI  L+  A                     R  L+   + + D+  TK    L+S     + A A              D++GL      L   D++    V D+  L GLT+  V A+      S+  +RPG AR +S A  +     ++S+NWS+ ++  VL                                        + +N+LV+   GVSTG++  +I  +  K R    L    R S+              +YVGDS +D+L +L AD GI++G++    K   AFG+ ++PL S                     G  +Y AS W EI  FLFG
Sbjct:   29 DIFSGLPVPGGLSERLWADSQAEAMEALHHPFVQALAAGTLDRACFQHYVLQDLFFLTRFARAYAAAIDKCGTTPALAAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSQYTDFLMQVATDPETSVAEVLAAMIPCARLYGFLGCQLAAATQGLPRHAYSEWVATYSGAEYLASPVMKEELFDKIAAAEPYDRLKQLYTRAMQLEVNFFSAQPNGPAP-------------------------------------RHVGLLVCDFDDTITEKDTIGTLLGAAVEXXXXXXXXXXXXXXXXXXXRQQLNTQVKNLADNYMTKQQQLLASLLPADDDASAAAAGVSNSVTNKSYDKDGLLAFCEALSDFDVEMNQVVVDAGCLAGLTKAGVIAAA-----SNITLRPGSARTLSAAAAAGLPVALLSVNWSKSLMQAVLQPAAXXXX--------XXXXXXXXXXXXXXXXXXXXXXMEIHANELVWGPDGVSTGDLQREIQCARDKGRVMDTLLQQHRSSSXXXXXXXXXXXXAVVYVGDSSSDILPLLKADYGIIIGSNKLLRKALAAFGVGLQPLCSAPMTPSSXXXXXXXXXXXXXEGPVLYEASSWQEIAAFLFG 607          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A7J7QPC3_9CHLO (Thiamine phosphate synthase n=1 Tax=Scenedesmus sp. NREL 46B-D3 TaxID=2650976 RepID=A0A7J7QPC3_9CHLO)

HSP 1 Score: 251 bits (641), Expect = 6.650e-65
Identity = 197/630 (31.27%), Postives = 282/630 (44.76%), Query Frame = 2

Query:  380 DVGKGFTLPGSL----WQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVL----EELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTK----LSSTACTTNGAGAV-------------DREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSG-----QCVYRASQWAEIDVFLFG 2179
            D+  G  +PG L    W+D Q EAM +LHHP+   LA G     + K YV QD +FL  FA+AYA A+ K      + A  +++  +L     EL LH SY+AKWG+ V     P  AT+ Y D+LM VA+DP +SVA+VLAAM+PC RLY FLG +L  A       AY+EW+ETYS  ++  +  + E L DK +    Y  L  LY +AM+LE  FF AQ +G                                        + + LL  DFDDT+TE DTI +L+  A                     R  L+   +T+ D+  TK    L+S     +G  A              D++GL      L   D++    V D+  L GLT+ +V A+  G       +RPG  R +S A  +     V+S+NWS+ ++  VL  T               +                     + +N+L +   GVSTG++  ++     K R  +D      +S++SS  +     +YVGDS +D+L +L AD GIV+G +    +   AFG+ ++PL S           P   A S          +Y AS W EI  FLFG
Sbjct:   56 DMFSGLPVPGGLSEQLWRDTQAEAMEALHHPFVQALAAGTLDRWA-KHYVLQDLFFLTRFARAYAAAIDKCAHSPHLGAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSAYTDYLMEVATDPETSVAEVLAAMIPCARLYGFLGCQLAAATRGQPRHAYSEWVETYSGAEYLASPVIKEELFDKIAHAVPYGRLKQLYARAMQLEVNFFSAQPNGPAP-------------------------------------RHVGLLVCDFDDTITEKDTIGVLMAAAVEANVKSSXXXXXXXXXXXXLRQQLNAQVKTLADNYMTKQQQLLASLLPADDGKPAAAMSVTNSVNNKTYDKDGLLAFCEALSDFDVEMNQVVVDAGCLAGLTKADVAAAAGGIA-----LRPGSMRTLSAAAAAGLPVAVLSVNWSRSLMEAVLQPT---------------AAAXXXXXXXXXXXXXXXXXLEIYANELAWGPEGVSTGDLRREVQCGRDKGR-VMDALLKQHQSSSSSRGA----VVYVGDSSSDILPLLKADYGIVIGGNRLLHRALEAFGVSLQPLCSA----------PMTPASSXXXXXXXXSVLYAASSWQEIAAFLFG 612          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A0G4GYE5_VITBC (TENA_THI-4 domain-containing protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4GYE5_VITBC)

HSP 1 Score: 238 bits (608), Expect = 4.510e-64
Identity = 198/608 (32.57%), Postives = 286/608 (47.04%), Query Frame = 2

Query:  413 LWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAE--DEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGKELRHAF--PNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAME------LEFAFFDAQ-LDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQ--LALLCVDFDDTLTEGDTISLLVETAKAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVV----GNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSGQCVYRASQWAEIDVFLFGGE 2185
            LWQ C+  A++SL+HPW L L     +  S+  ++ QDA+FL  FAKAYA+AL K    D E IRAF++LIG VL EL LH S S +W ID  +T  P +AT  Y  FL  V +D  ++ A VLA+M PCM LYAF+GK LR            Y EW+ TYSS +F   A  VE LLD+ +       L +L  +  E      L + + +A  L+ G  DA   Q++             P    +    R  G+ Q+  L+ + +DFD T T+ DT  LL E A      P DQV   + W  +   F  ++   + + L  K+                GLE  L  L   +  +V+ VS++++L+G TRD+V   +   LR  W+VR      +S  L       ++S++WSQD+I  +L +                       G   S       P + +S     D    +TGEI+  + G+  K R F  L    RE     +     +++++GDSVTDLLA+L+AD+GIV+      + + +KV   +GID++PL           E       S     +Y A  W EI V LFG E
Sbjct:   53 LWQKCRRRALKSLYHPWVLRLGASVLARESYVEFMRQDAFFLEIFAKAYAHALCKCPPGDVEGIRAFNALIGGVLSELTLHQS-STEWAIDT-STFTPNEATVRYTSFLRDVGADQWTTTAMVLASMAPCMSLYAFIGKALREGVGEEGMAGHRYNEWLATYSSAEFAQDAARVEELLDRYT-----DPLLSLSGQVGEDAVRGNLLYYYGNAMDLEYGFFDA---QAL-----------PPPSCAPSASPSRHPGLAQRGVLSFVAIDFDGTCTQRDTTPLLHEVAAEVHSDP-DQVR--QRWRAIEDAFFDKYRRKVGELLEGKVRD-----------GMNGLEAFLGGLGEFEKAAVADVSEARLLQGFTRDDVRRMVKTSLRQKWQVRDACGEVLSGLLSHDVPASIISVSWSQDMIHTILQDA---------------------CGAPLS------LPITCNSFSFREDDQR-ATGEISSHMTGAVDKLRAFRRL----RE----EKGREGRLSVFIGDSVTDLLALLEADIGIVLKDALNKNNTLDKVISLYGIDVQPLVRAAMIAQCGQEAATVTPVSMPPMTIYAADGWDEIGVMLFGNE 589          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A2K3DGU0_CHLRE (Thiamine phosphate synthase n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3DGU0_CHLRE)

HSP 1 Score: 245 bits (626), Expect = 3.210e-63
Identity = 213/628 (33.92%), Postives = 283/628 (45.06%), Query Frame = 2

Query:  401 LPGSLWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAE--DEEDIRAFHSLIGSVLEELGLHASYSAKWGID--VANTPPPVKATAEYVDFLMAVAS--DPTSSVAQVLAAMVPCMRLYAFLGKELRHAF-PNWTESA-----YAEWIETYSSEDFEDAAGLVESLLDKNSA-GADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQ-----RETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLD----GDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDL----------AAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSGQCVYRA-SQWAEIDVFLFGGE 2185
            L   LW + Q E   SLH P+  G+A G     +F+ YVAQDAYFL  FA+AY  AL+KA   DE+       L+  V EEL LH SY+AKWG+D  VA T P   AT  Y DFLM VA       SVA++LAAM+PC RLY FLG  L  A  P   + A     Y EW++TYSS+++    GL E + ++ +  GAD   L ALYR+AM+LE  FF AQ                        S  P               ++ + L VDFD+T T  DTI  L+  A A      R T  D   +      L   +++  GE +   L        C        D  GLE +L  L   D      V +  +LRG T  EV A+ AG      ++RP     ++ AL      HV+S+NWS   +   LS                    R+ AGE           G     +  N L  D  G++TG +  ++  +  K     +L          AA +  S  S  SS     +YVGDS +DL AMLD+D G+V+G++    +V R FG+ I PLA+          L  R  P+G+   +Y A   WAEI  FLFG E
Sbjct:   15 LAKELWDNVQHEVFMSLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDEQTFEDLTGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSA-ATKAYTDFLMDVADAGGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPCHDYWEWVKTYSSQEYLSVPGLKERMFERLAGKGADKAKLLALYRRAMQLEAEFFAAQP----------------------YSPPP---------------RRFSALVVDFDETCTARDTIGGLMRLAAAAAGQPGRATAGDAAWVSATSARLADAYVAAQGELLGRLLPEGAPDATC-------YDAAGLEALLAALSDFDAAMNGPVEECGILRGSTAAEVAAAGAG-----LQLRPQCREVLAGALGRGIPVHVLSVNWSDVYVGAALSAAAXXXXXXXXXXXXXXLPVRL-AGEXXXXXXXXXXXGPAAVELLCNSLQLDGSGLTTGALTKRVQSARDKRAALAELRARLPAASTAAAAADGSTDSGSSSNPAALVYVGDSTSDLGAMLDSDFGVVIGSNALLRRVCRRFGVRILPLAAA--------PLDPRRVPAGT---LYEAVGGWAEIRAFLFGVE 580          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A836B2K3_CHLIN (Thiamine phosphate synthase n=1 Tax=Chlamydomonas incerta TaxID=51695 RepID=A0A836B2K3_CHLIN)

HSP 1 Score: 241 bits (615), Expect = 6.850e-62
Identity = 213/626 (34.03%), Postives = 283/626 (45.21%), Query Frame = 2

Query:  401 LPGSLWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAE--DEEDIRAFHSLIGSVLEELGLHASYSAKWGID--VANTPPPVKATAEYVDFLMAVAS--DPTSSVAQVLAAMVPCMRLYAFLGKELRHAF-PNWTESA-----YAEWIETYSSEDFEDAAGLVESLLDKNSAG-ADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQ-----RETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRL---DGDCPA-SVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDL--------AAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDKTGELPRRAAPSGSGQCVYRA-SQWAEIDVFLFGGE 2185
            L   LW + Q E   SLH P+  G+A G     +F+ YVAQDAYFL  FA+AY  AL+KA   D+        L+  V EEL LH SY+AKWG+D  VA T P   AT  Y DFLM VA       SVA++LAAM+PC RLY FLG  L  A  P   + A     Y EW+ TYSS D+    GL E + ++ +   AD   L  LYR+AM+LE  FF AQ                        S  P               ++ + L VDFD+T T  DTI  L+  A A      R T  D   +      L   +++  GE +   L             A A D  GL+  L  L   D    + V    +LRG T  EV A+ AG      ++RP     +S AL      HV+S+NWS   +   L+           +T  +              RL   +G  PA  +  N L  D  G++TG +A ++  +  K     +L        AA + + A+ S S      +YVGDS +DL AMLD+D G+V+G++    +V R FGI I PLA+          L  R  P+G+   +Y A   WAEI  FLFG E
Sbjct:   15 LAKELWDNVQHEVFMSLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDDSTFEDLSGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSA-ATKAYTDFLMNVADAEGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPAHHYWEWVRTYSSADYLSVPGLKERMFERLAGKEADKAKLLRLYRRAMQLEAEFFAAQP----------------------YSPPP---------------RRFSALVVDFDETCTAKDTIGGLMRLAAAAAGQQGRATAGDAAWVAATSSRLAADYVAAQGELLGRLLPEGAPD-------AAAYDAAGLDAFLAALSDFDAAMNAPVEACGILRGSTSAEVAAAGAG-----LQLRPQCREVLSGALGRGIPVHVLSVNWSDVYVGAALAAP---------ATAAVXXXXXXXXXSALPVRLAGAEGAGPAVELRCNSLELDGAGLTTGALAKRVQSARDKRAALAELRSRLPAAPAAAAVDGASDSGSDNPAALVYVGDSTSDLGAMLDSDFGVVIGSNALLRRVCRRFGIRILPLAAA--------PLDPRRVPAGT---LYEAVGGWAEIRAFLFGVE 570          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: A0A8J5XNG5_DIALT (Thiamine phosphate synthase n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XNG5_DIALT)

HSP 1 Score: 240 bits (613), Expect = 1.240e-61
Identity = 188/563 (33.39%), Postives = 276/563 (49.02%), Query Frame = 2

Query:  401 LPGSLWQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDQVDIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCIS--RALVSPFQTHVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEI--AVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAM 2077
            L   LW      A RSLH P+ L LA G    ASF+ YVAQDA+FLRAFA+AYA AL++ E +  I    SLI  V++EL LH  Y+AKW +D+A   P   AT+ Y +FL+A+A    + +A VLAAM PCMRLYAFLG+ L  AFP+    AYA+W+ TYSS++F   AG +E+LLD+ + G    +  A Y +AM+LE  FF +Q      D +L     A+R                             L  VDFD+T T+  T+ LL + A       +++     EW  L+ TF  E+   IE  +       A     A  V+      M+ +L   +  +V      + LRG+      A+LA       K+R G AR     RA  +  +  V+S+NW+   +AR      LD+         + +G  + A E      + + P ++ ++D        +TGE+  A  +  S  K   F  L A    + A +  +  L+T+Y GDS+TDL  +L A +GIV+G+S S      + G+ ++PL +   A+
Sbjct:    5 LSSKLWASALPLATRSLHSPFVLRLAAGTLPRASFQGYVAQDAFFLRAFARAYALALSRCESDGAIGELSSLIAGVVDELALHHGYAAKWDLDLAGVRP-TAATSAYTEFLLALARS-DAPIAVVLAAMTPCMRLYAFLGQRLCAAFPD-ASHAYADWLRTYSSDEFGALAGRIEALLDECADGVPLDAARAAYTRAMQLELDFFASQPGVAPVDGLLAP---ADR----------------------------LLFAVDFDETATDAQTLPLLAQLAAEHAGAADERAARVAEWRTLSETFAREYTMAIERIVPPXXXXXAPPAGEAAVVE------MVERLSAFERRAVEAAERGRALRGVPE----AALAKAGAERVKLRDGFARLAYALRAAGACERAEVISVNWAAQ-LARAALRARLDA------ARPVGAGATVGAEELVRLHCN-EMPVAIDAHDGAPR----TTGELRAAAGLHTSADKGAAFGQLCA--EYARAHAGGAGELLTLYAGDSITDLQPLLAARIGIVLGHSSSLRAALSSLGVRVQPLVAARRAL 509          

BLAST of mRNA_E-fasciculatus_F_contig1067.532.1 vs. uniprot
Match: U5D054_AMBTC (Uncharacterized protein n=2 Tax=Amborella trichopoda TaxID=13333 RepID=U5D054_AMBTC)

HSP 1 Score: 227 bits (578), Expect = 2.470e-60
Identity = 188/632 (29.75%), Postives = 283/632 (44.78%), Query Frame = 2

Query:  416 WQDCQLEAMRSLHHPWTLGLATGQTSLASFKSYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVAS----------------DPTSSVAQVLAAMVPCMRLYAFLGKELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNS---AGADYPSLFALYRKAMELEFAFFDAQLDGGTEDAVLEQSVGAERGGRQGMSVQPGGQDAGGRGRFYGVHQQLALLCVDFDDTLTEGDTISLLVETA-----KAQRETPEDQV------DIGREWGLLTRTFLSEWGETIEDSLSTKLSSTACTTNGAGAVDREGLEVMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHASLAGPLRSSWKVRPGMARCISRALVSPFQ------------THVVSINWSQDVIARVLSETLLDSHSSLSSTTGIDSGGRIWAGETASPRLDGDCPASVSSNDLVFDKHGVSTGEIAVKIPGSFGKHRRFLDLAAMSRESAASSESSPRLMTIYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLAS--VYEAMDKTGELPRRAAPSGSGQCVYRASQWAEIDVFLFG 2179
            W     E++ + + P+ + LA G   L +F+ YVAQDA+FL+ FA+AY  A   A+D++   +   L   VLEEL +H SY+ +WG+D+     P  AT +Y+DFLMA A+                + T   A  + AM PCMRLYAFLGKEL+  F N  +  Y +WI+ YSS+ FE AA  +E LLDK S    G +   +  LY +AM+LE  FF AQ    ++  ++  S                          +   +Q  ++  DFD T T  D+ ++L E A     K ++   E  +      D+   WG L++ +  E+ + +ED +          ++ A   D +GL   L  L   +  + SRV++S VL+GL  +++              R G    +     S FQ             HV+S  W  D+I    S  LLD                                 S+ SN+ +++   VSTG I  ++     K R F D+     E         + +++Y+GDSV DLL +L+ADVGIV+G+S S  +V   FG+   PL S  V +  D   +    +   G    +Y  S WAEI  F+ G
Sbjct:   14 WVKFLRESVFASYSPFIVCLAAGNLELDTFRHYVAQDAHFLKCFARAYEMAEDCADDDDAKASICELRKGVLEELKMHESYAEEWGVDLVKESTPSSATTKYIDFLMATAAGKVEGGKGPSRIATPFEKTKIAAYTVGAMTPCMRLYAFLGKELQR-FVNSADHQYKKWIDIYSSDSFEVAALQIEELLDKLSISLTGEELEVIERLYHQAMKLEIEFFSAQPI--SQKTLVPLS------------------------NVHKPSEQRLIIFSDFDLTCTVVDSSAILAEIAIRTAPKVEQNGSEQPIARQSSADLRNSWGALSQQYTEEYEQCVEDIVP---------SDEAEVFDYQGLCKALEHLSEFEKRANSRVNESGVLKGLNLEDIK-------------RAGERLILQDGCTSFFQQIVKRKDELNADVHVLSYCWCGDLIRSAFSSGLLD-------------------------------VLSIHSNEFIYEG-SVSTGGIVREVESPMDKSRVFKDVLESIGE-------PDKRVSVYIGDSVGDLLCLLEADVGIVIGSSSSLRRVGSKFGVSFVPLLSGVVKKQRDFLEDSCSSSNWKGLSGTLYTVSSWAEIHAFILG 557