mRNA_E_fasciculatus_S2_contig9368.17419.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig9368.17419.1
Unique NamemRNA_E_fasciculatus_S2_contig9368.17419.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: CDGSH (iron sulfur domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LP22_ECTSI)

HSP 1 Score: 187 bits (476), Expect = 1.110e-59
Identity = 96/109 (88.07%), Postives = 98/109 (89.91%), Query Frame = 1 
Query:   49 MKTACAIALVTVGCANAFVMPAGRTVVRAETARAAEKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            MKTA AIALVT+GCANAFV+PAGRTVVRAETARAAEKINTIEFE DKPK         VVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK
Sbjct:    1 MKTAGAIALVTLGCANAFVIPAGRTVVRAETARAAEKINTIEFELDKPK---------VVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 100
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Hypothetical (protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2GPT9_9STRA)

HSP 1 Score: 106 bits (264), Expect = 2.930e-27
Identity = 53/82 (64.63%), Postives = 59/82 (71.95%), Query Frame = 1 
Query:  130 RAETARAAEKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            RA   +AAE INTI FE D PK         VVT   ++AG KKV+CRCWKSGTFPLCDG+HV HNEATGDNVGPLIV+  K
Sbjct:   42 RARLVQAAEPINTIGFEPDSPK---------VVTNLKLEAGEKKVFCRCWKSGTFPLCDGSHVPHNEATGDNVGPLIVAAEK 114
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Hypothetical (protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2UEE3_9STRA)

HSP 1 Score: 101 bits (251), Expect = 1.790e-25
Identity = 50/81 (61.73%), Postives = 59/81 (72.84%), Query Frame = 1 
Query:  133 AETARAAEKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            A T   AE+INT + + D PK         V TM+ ++ G KKVYCRCW+SGTFPLCDG+H+KHNEATGDNVGPLIVS PK
Sbjct:   27 ASTKLFAERINT-QVDLDSPK---------VATMEKLEPGTKKVYCRCWQSGTFPLCDGSHMKHNEATGDNVGPLIVSVPK 97
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Hypothetical (protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JTK3_9STRA)

HSP 1 Score: 101 bits (251), Expect = 2.820e-25
Identity = 43/56 (76.79%), Postives = 47/56 (83.93%), Query Frame = 1 
Query:  208 IERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            ++   VVTMD ++AG KKVYCRCW SGTFPLCDG H KHNEATGDNVGPLIVS PK
Sbjct:   59 LDSAKVVTMDELEAGKKKVYCRCWLSGTFPLCDGTHAKHNEATGDNVGPLIVSSPK 114
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: ZnF_CDGSH (domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DCC5_9STRA)

HSP 1 Score: 99.0 bits (245), Expect = 1.500e-24
Identity = 53/95 (55.79%), Postives = 63/95 (66.32%), Query Frame = 1 
Query:   91 ANAFVMPAGRTVVRAETARAAEKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            A+AF  P+    V   +   A KINT + E + PK         VVTM  +  G KKV+CRCW+SGTFPLCDGAH+KHNEATGDNVGPLI+S PK
Sbjct:   17 ADAFFKPS----VGRRSLELASKINT-KVELENPK---------VVTMTQLAPGEKKVFCRCWQSGTFPLCDGAHMKHNEATGDNVGPLIISNPK 97
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Hypothetical (protein n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1GHV5_CYCTE)

HSP 1 Score: 97.8 bits (242), Expect = 7.920e-24
Identity = 42/56 (75.00%), Postives = 47/56 (83.93%), Query Frame = 1 
Query:  208 IERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            +E   VVTM+++   GKKVYCRCW+SGTFPLCDG H KHNEATGDNVGPLIVS PK
Sbjct:   64 LESAKVVTMETLAEPGKKVYCRCWQSGTFPLCDGTHAKHNEATGDNVGPLIVSVPK 119
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: CDGSH (iron sulfur domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBW8_9STRA)

HSP 1 Score: 95.5 bits (236), Expect = 3.100e-23
Identity = 56/107 (52.34%), Postives = 71/107 (66.36%), Query Frame = 1 
Query:   49 MKTACAIALV-TVGCANAFVMPAGRTVVRAETARAAEKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVS 366
            M+T  A+AL+ +V    AFV  +    +RA T   +E+IN  + + + PK         V TM+++ AGGKKVYCRCWKS TFPLC+GAHVKHNE  GDNVGPLIVS
Sbjct:    1 MQTVFAVALLASVASVPAFVPSSS---IRAPT-HLSERINN-KIDLESPK---------VATMETLPAGGKKVYCRCWKSATFPLCNGAHVKHNEEVGDNVGPLIVS 93
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Hypothetical (protein n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2I006_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 3.280e-23
Identity = 44/71 (61.97%), Postives = 52/71 (73.24%), Query Frame = 1 
Query:  154 EKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVS 366
            +K   I FE D       ++   VVT + ++AG KKVYCRCW+SGTFPLCDG+HVKHNE TGDNVGPLIVS
Sbjct:   55 DKPGKINFEKD-------LDSPKVVTQEKLEAGDKKVYCRCWQSGTFPLCDGSHVKHNEETGDNVGPLIVS 118
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Iron-sulfur (domain-containing NEET-like protein n=2 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EJJ5_9STRA)

HSP 1 Score: 95.1 bits (235), Expect = 5.980e-23
Identity = 44/67 (65.67%), Postives = 51/67 (76.12%), Query Frame = 1 
Query:  190 PKASKI-----IERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVSGPK 375
            P+ASKI     ++   VV M+ +DAG K VYCRCWKSGTFPLCDG HV HN+ATGDNVGPLIV+  K
Sbjct:   39 PEASKINTLIDLDAEKVVNMEKLDAGKKAVYCRCWKSGTFPLCDGNHVAHNKATGDNVGPLIVTAAK 105
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BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Match: Cisd1 (protein n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812JQ04_9DINO)

HSP 1 Score: 95.1 bits (235), Expect = 9.590e-23
Identity = 54/93 (58.06%), Postives = 62/93 (66.67%), Query Frame = 1 
Query:   91 ANAFVMPAGRTVVRAETARAAE-KINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPLCDGAHVKHNEATGDNVGPLIVS 366
            A  F++ AGR +    TAR AE KIN  + + + PK         VVT + + AG KKVYCRCW SGTFPLCDG H KHNEATGDNVGPLIVS
Sbjct:   40 AAGFLVLAGRQL---RTARKAEGKINN-KIDPEVPK---------VVTKEDLKAGDKKVYCRCWLSGTFPLCDGTHAKHNEATGDNVGPLIVS 119
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig9368.17419.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
CDGSH1.110e-5988.07iron sulfur domain-containing protein n=1 Tax=Ecto... [more]
Hypothetical2.930e-2764.63protein n=1 Tax=Dictyocha speculum TaxID=35687 Rep... [more]
Hypothetical1.790e-2561.73protein n=1 Tax=Attheya septentrionalis TaxID=4202... [more]
Hypothetical2.820e-2576.79protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 ... [more]
ZnF_CDGSH1.500e-2455.79domain-containing protein n=1 Tax=Nannochloropsis ... [more]
Hypothetical7.920e-2475.00protein n=1 Tax=Cyclophora tenuis TaxID=216820 Rep... [more]
CDGSH3.100e-2352.34iron sulfur domain-containing protein n=1 Tax=Trib... [more]
Hypothetical3.280e-2361.97protein n=1 Tax=Helicotheca tamesis TaxID=374047 R... [more]
Iron-sulfur5.980e-2365.67domain-containing NEET-like protein n=2 Tax=Fragil... [more]
Cisd19.590e-2358.06protein n=1 Tax=Symbiodinium natans TaxID=878477 R... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig9368contigE_fasciculatus_S2_contig9368:2271..3885 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score187.6
Seed ortholog evalue3.6e-45
Seed eggNOG ortholog2880.D8LP22
Model size378
Hectar predicted targeting categoryother localisation
GOsGO:0000422,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005741,GO:0005783,GO:0005789,GO:0006914,GO:0006996,GO:0007005,GO:0007275,GO:0007568,GO:0008150,GO:0008152,GO:0009056,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009894,GO:0009987,GO:0010150,GO:0010259,GO:0010506,GO:0012505,GO:0016020,GO:0016043,GO:0019222,GO:0019867,GO:0022411,GO:0031090,GO:0031323,GO:0031329,GO:0031966,GO:0031967,GO:0031968,GO:0031975,GO:0031984,GO:0032501,GO:0032502,GO:0042175,GO:0042592,GO:0042802,GO:0042803,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044248,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044432,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0046983,GO:0048037,GO:0048366,GO:0048367,GO:0048731,GO:0048827,GO:0048856,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0051536,GO:0051537,GO:0051540,GO:0055065,GO:0055072,GO:0055076,GO:0055080,GO:0061726,GO:0061919,GO:0065007,GO:0065008,GO:0071840,GO:0072593,GO:0090693,GO:0098588,GO:0098771,GO:0098805,GO:0098827,GO:0099402,GO:1903008
Exons3
EggNOG free text desc.2 iron, 2 sulfur cluster binding
EggNOG OGsKOG3461@1,KOG3461@2759
Ec32 ortholog descriptionIron sulphur-containing domain, CDGSH-type, subfamily
Ec32 orthologEc-06_006890.1
Cds size378
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842586.3406606-CDS-E_fasciculatus_S2_contig9368:2270..24921696842586.3406606-CDS-E_fasciculatus_S2_contig9368:2270..2492Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9368 2271..2492 +
1696842586.3576524-CDS-E_fasciculatus_S2_contig9368:3133..31831696842586.3576524-CDS-E_fasciculatus_S2_contig9368:3133..3183Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9368 3134..3183 +
1696842586.3674242-CDS-E_fasciculatus_S2_contig9368:3779..38851696842586.3674242-CDS-E_fasciculatus_S2_contig9368:3779..3885Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9368 3780..3885 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig9368.17419.1prot_E_fasciculatus_S2_contig9368.17419.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig9368 2271..3885 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig9368.17419.1

>prot_E_fasciculatus_S2_contig9368.17419.1 ID=prot_E_fasciculatus_S2_contig9368.17419.1|Name=mRNA_E_fasciculatus_S2_contig9368.17419.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=126bp
PAEATHTTNIPDFTTTMKTACAIALVTVGCANAFVMPAGRTVVRAETARA
AEKINTIEFEADKPKASKIIERTAVVTMDSVDAGGKKVYCRCWKSGTFPL
CDGAHVKHNEATGDNVGPLIVSGPK*
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mRNA from alignment at E_fasciculatus_S2_contig9368:2271..3885+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig9368.17419.1 ID=mRNA_E_fasciculatus_S2_contig9368.17419.1|Name=mRNA_E_fasciculatus_S2_contig9368.17419.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=1615bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig9368:2271..3885+ (Ectocarpus fasciculatus EfasUO2)
CCCGCCGAAGCCACGCACACAACAAACATCCCTGATTTTACCACGACCAT
GAAGACTGCTTGCGCTATCGCACTCGTCACCGTTGGCTGTGCCAATGCCT
TTGTCATGCCCGCGGGCCGCACGGTAGTGCGCGCGGAAACAGCTCGAGCC
GCTGAGAAAATCAACACGATCGAGTTCGAGGCGGACAAGCCGAAGGCAAG
CAAAATCATCGAGCGGACTGCTGTGTACATGCTACCTGCTGTGCTACTGC
TGTACAGCAGTAGTCTACTGCTGTAGTGAGGCGTAAGTCCTCCATTGTTG
CCTGTTGCAAGTGGCACCGACTTGCGCGCGCCATCTCAGCCTAGTCACTC
ACCCATTTCCATGCGTCGTGTTGCTCTCATGGTTGAGCCACCTCTGCAGC
AGATCTCATGTATCATCGATGTTCATGTTCACTTCCGACTTGCTGCTGGC
ACAACGTGCTATGCATACATGTGTACCTGCAATGCGGTGGGTGTTTCTCT
TCCCGGCTTCCCGGTATCCTACGTACAGTAGTAGGAGGGGCAGCGTGGAC
AACACGACCCCGTGAGCTCAGGGATAACAATGCGATTGAGAGACACGTAT
GCTGCCATTCCTGTGTTTTCCCTGACTGAAGACACCCACTCTGTACACGT
GCACCACAGGAGCACAGGGGCCCCGAGCCCTGAGTCAACTACAACATGAG
CACGAGAGCAAGTGAAAAGAGGAATGTACTGCTGTAATGCGTCGTCCTAT
ACACACAGCAGCAGCTGATACTTTTTCATCGTCTACTTTTATGAAGTATA
TTAGAACTCACGTGGTATGTGTGCCTGCCCTTCCACGAAACGCGACGCGC
CAAAACATTTCAGGTTGTAACGATGGACTCTGTGGACGCCGGGGGCAAGA
AAGTGTACTGCCGGTGAGGACGTCTGGAAAATAACACACTACAGTTTGTA
ACTACGTCAGTTGTGCTCTCGGAAAATCTACCCACAGCTGTAGTACAGTA
CACGTGTTCTAGTCTACGCGTACGAAGTGTGTAGGGTTCATATGACCATA
TGGCCATTTCATGTGTTCTTGGCGTAAGCATGCGTCTCTGTTTTCAAGTG
TTCCATTGTTTTCTTGCATCCTGTCCATGAACCGTCGGTCGAAATACCTG
CCCACCTTCAGCAATATTTCATGTCGAGCCTCTCTACAATACAAAGAGCT
CCGATCCGGGGTCTTCCAAAGGACGATCCTGATTGTTTGCTTCATTTTCT
TCTTGTTTTTCGTGGGAACGATTCAGCATTCCCTTCGGTGGTAAGTTTAA
GCTGCTTAATTTACTCACGAACGGCTAGGTTTTTGCCACTATGCATAGGA
ATATAGTGTGGAAACCGGTTACCTTCGCACCAGATGCCAGCACACGGCTC
GCCGCGCCGCTCTAATCACTCTCGTATCTGTTGGCGGCGCTCTCTCTCTC
TCTCTGCCTTACTTTGCGCAACTCCCCTGTTGTTTGCGGGCGTGGCACAT
CAAAAAAAGGTGCTGGAAATCCGGGACCTTCCCCCTGTGCGACGGTGCCC
ACGTGAAGCACAACGAGGCCACCGGAGACAACGTGGGCCCGCTCATTGTG
TCCGGTCCCAAGTAA
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig9368:2271..3885+

>mRNA_E_fasciculatus_S2_contig9368.17419.1 ID=mRNA_E_fasciculatus_S2_contig9368.17419.1|Name=mRNA_E_fasciculatus_S2_contig9368.17419.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=378bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig9368:2271..3885+ (Ectocarpus fasciculatus EfasUO2)
CCCGCCGAAGCCACGCACACAACAAACATCCCTGATTTTACCACGACCAT
GAAGACTGCTTGCGCTATCGCACTCGTCACCGTTGGCTGTGCCAATGCCT
TTGTCATGCCCGCGGGCCGCACGGTAGTGCGCGCGGAAACAGCTCGAGCC
GCTGAGAAAATCAACACGATCGAGTTCGAGGCGGACAAGCCGAAGGCAAG
CAAAATCATCGAGCGGACTGCTGTTGTAACGATGGACTCTGTGGACGCCG
GGGGCAAGAAAGTGTACTGCCGGTGCTGGAAATCCGGGACCTTCCCCCTG
TGCGACGGTGCCCACGTGAAGCACAACGAGGCCACCGGAGACAACGTGGG
CCCGCTCATTGTGTCCGGTCCCAAGTAA
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