mRNA_E_fasciculatus_S2_contig8247.16394.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig8247.16394.1
Unique NamemRNA_E_fasciculatus_S2_contig8247.16394.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12, chloroplastic n=6 Tax=Phaeophyceae TaxID=2870 RepID=D1J716_ECTSI)

HSP 1 Score: 192 bits (489), Expect = 3.470e-61
Identity = 128/130 (98.46%), Postives = 128/130 (98.46%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXX N SGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK
Sbjct:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXXNASGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 130
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BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=A0A6G6D787_ECTSI)

HSP 1 Score: 191 bits (486), Expect = 9.940e-61
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDAS+GXXXXXXXXXXXXXX N SGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK
Sbjct:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASSGXXXXXXXXXXXXXXXNASGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 130
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12, chloroplastic n=1 Tax=Pleurocladia lacustris TaxID=246121 RepID=A0A1I9LVX1_9PHAE)

HSP 1 Score: 177 bits (449), Expect = 4.440e-55
Identity = 106/131 (80.92%), Postives = 113/131 (86.26%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDAS-AGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MT+KISTLIDELKTLTLLEAAELVKAIEETFDVDAS AG              DN S EEKTEFDVSLDEVPKDKKISILKI+RS+TELGLKEAKEVVENTPKVI+EGL+K+AAEE KK+LEEAGAVVTLK
Sbjct:    1 MTKKISTLIDELKTLTLLEAAELVKAIEETFDVDASPAGGGVMMMNAGATISEDNVSDEEKTEFDVSLDEVPKDKKISILKIIRSITELGLKEAKEVVENTPKVIKEGLSKTAAEETKKLLEEAGAVVTLK 131
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BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: Ribosomal (protein L12 n=1 Tax=Desmarestia aculeata TaxID=62298 RepID=A0A8F0FB01_9PHAE)

HSP 1 Score: 176 bits (446), Expect = 1.230e-54
Identity = 117/130 (90.00%), Postives = 123/130 (94.62%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLI+ELKTLTLLEAAELVKAIEETFDVDASAG XXXXXXXXXXXXX     EEKTEFDVSLDEVPKDKKISILK+VRS+TELGLKEAKEVVENTPKVI+ GLTKSAAEEAKK+LE+AGAVVTLK
Sbjct:    1 MTEKISTLIEELKTLTLLEAAELVKAIEETFDVDASAGGXXXXXXXXXXXXXXXXXAEEKTEFDVSLDEVPKDKKISILKVVRSVTELGLKEAKEVVENTPKVIKAGLTKSAAEEAKKLLEDAGAVVTLK 130
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12, chloroplastic n=28 Tax=Laminariales TaxID=2886 RepID=A0A5B9RE57_9PHAE)

HSP 1 Score: 174 bits (442), Expect = 5.000e-54
Identity = 116/130 (89.23%), Postives = 123/130 (94.62%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXX   + EEKTEFDVSLDEVPKDKKI ILK+VRS+TELGLKEAKE+VENTPKVI+EGLTK+AAEE KK LE+AGAVVTLK
Sbjct:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXXGEAAEEKTEFDVSLDEVPKDKKIPILKVVRSVTELGLKEAKELVENTPKVIKEGLTKAAAEETKKTLEDAGAVVTLK 130
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12 n=1 Tax=Cladosiphon okamuranus TaxID=309737 RepID=A0A6B7EZL5_9PHAE)

HSP 1 Score: 173 bits (439), Expect = 1.480e-53
Identity = 118/131 (90.08%), Postives = 125/131 (95.42%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGS-GEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXX     GEEKTEF+VSLDEVPKDKKISILK+VR++TELGLKEAKEVVENTPKVIQEGLTK+AAEE KK+LE+AGAVVTLK
Sbjct:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXXXXXXGEEKTEFNVSLDEVPKDKKISILKVVRAVTELGLKEAKEVVENTPKVIQEGLTKAAAEETKKLLEDAGAVVTLK 131
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: Ribosomal (protein L12 n=1 Tax=Postelsia palmaeformis TaxID=105414 RepID=A0A8F0F840_9PHAE)

HSP 1 Score: 172 bits (436), Expect = 4.100e-53
Identity = 101/130 (77.69%), Postives = 108/130 (83.08%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLID LKTLTLLEAAELVKAIEETFDVDAS G              +  + EEKTEFD+SLDEVPKDKKI ILKIVRSLTELGLKEAKE+VENTPKVI+EGLTK+AAEE KK LE+AGAVVTLK
Sbjct:    1 MTEKISTLIDGLKTLTLLEAAELVKAIEETFDVDASTGGGVMMMSQGGSVSDNGETAEEKTEFDISLDEVPKDKKIPILKIVRSLTELGLKEAKELVENTPKVIKEGLTKAAAEETKKTLEDAGAVVTLK 130
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12, chloroplastic n=3 Tax=Fucaceae TaxID=3010 RepID=D1GJP6_FUCVE)

HSP 1 Score: 164 bits (416), Expect = 4.840e-50
Identity = 99/132 (75.00%), Postives = 109/132 (82.58%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXX--DNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKISTLI+ELKTLTLLEAAELVK+IEETFDVDA  G                DN + EEKTEFDVSL+EVPKDKKI+ILK+VRS+TELGLKEAKEVVENTPKVI+EGL K AAEEAKK+LE+AGAVV LK
Sbjct:    1 MTEKISTLIEELKTLTLLEAAELVKSIEETFDVDAPTGGASGVMMMNPGAISTEDNNTVEEKTEFDVSLEEVPKDKKIAILKVVRSVTELGLKEAKEVVENTPKVIKEGLPKVAAEEAKKLLEDAGAVVILK 132
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12, chloroplastic n=1 Tax=Ishige okamurae TaxID=233772 RepID=A0A8E5XRI1_9PHAE)

HSP 1 Score: 159 bits (403), Expect = 4.330e-48
Identity = 93/130 (71.54%), Postives = 105/130 (80.77%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            M EKIS LI+ELK LTLLEAA+LV AIEETF+VDASAG              D    EEKTEFD+SLDEVPKDKKISILK+VR++T+LGLKEAKEVVENTPK+++EGL K AAEEAK +LEEAGAVVTLK
Sbjct:    1 MNEKISPLIEELKNLTLLEAADLVTAIEETFNVDASAGGGVMMMGTTTSPSEDAKPTEEKTEFDISLDEVPKDKKISILKVVRTVTQLGLKEAKEVVENTPKIVKEGLLKEAAEEAKTLLEEAGAVVTLK 130
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Match: 50S (ribosomal protein L12, chloroplastic n=15 Tax=Sargassaceae TaxID=3014 RepID=A0A141BSK5_9PHAE)

HSP 1 Score: 159 bits (401), Expect = 9.580e-48
Identity = 110/133 (82.71%), Postives = 121/133 (90.98%), Query Frame = 1 
Query:    1 MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGXXXXXXXXXXXXXXD---NGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENTPKVIQEGLTKSAAEEAKKVLEEAGAVVTLK 390
            MTEKI TLI+ELKTLTLLEA ELV++IEETF+VDAS GXXXXXXXXXXXXXX    N + EEKTEFD+SLDEVPKDKKI+ILK+VRS+TELGLKEAKEVVENTPKVI+EGL K AAEEAKK LEEAGA+VTLK
Sbjct:    1 MTEKILTLIEELKTLTLLEATELVQSIEETFNVDASTGXXXXXXXXXXXXXXXXXXNKAIEEKTEFDISLDEVPKDKKIAILKVVRSVTELGLKEAKEVVENTPKVIKEGLAKPAAEEAKKSLEEAGAIVTLK 133
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig8247.16394.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
50S3.470e-6198.46ribosomal protein L12, chloroplastic n=6 Tax=Phaeo... [more]
50S9.940e-6197.69ribosomal protein L12 n=1 Tax=Ectocarpus siliculos... [more]
50S4.440e-5580.92ribosomal protein L12, chloroplastic n=1 Tax=Pleur... [more]
Ribosomal1.230e-5490.00protein L12 n=1 Tax=Desmarestia aculeata TaxID=622... [more]
50S5.000e-5489.23ribosomal protein L12, chloroplastic n=28 Tax=Lami... [more]
50S1.480e-5390.08ribosomal protein L12 n=1 Tax=Cladosiphon okamuran... [more]
Ribosomal4.100e-5377.69protein L12 n=1 Tax=Postelsia palmaeformis TaxID=1... [more]
50S4.840e-5075.00ribosomal protein L12, chloroplastic n=3 Tax=Fucac... [more]
50S4.330e-4871.54ribosomal protein L12, chloroplastic n=1 Tax=Ishig... [more]
50S9.580e-4882.71ribosomal protein L12, chloroplastic n=15 Tax=Sarg... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig8247contigE_fasciculatus_S2_contig8247:708..1100 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score198.4
Seed ortholog evalue2.1e-48
Seed eggNOG ortholog2880.D1J716
Preferred namerpl12
Model size393
KEGG koko:K02863,ko:K02935
KEGG Pathwayko03010,map03010
KEGG ModuleM00178,M00179
Hectar predicted targeting categoryother localisation
GOsGO:0000311,GO:0000313,GO:0000315,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005840,GO:0005911,GO:0006412,GO:0006518,GO:0006807,GO:0006950,GO:0006952,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009295,GO:0009506,GO:0009507,GO:0009526,GO:0009532,GO:0009534,GO:0009535,GO:0009536,GO:0009547,GO:0009570,GO:0009579,GO:0009605,GO:0009607,GO:0009617,GO:0009719,GO:0009725,GO:0009735,GO:0009941,GO:0009987,GO:0010033,GO:0010467,GO:0015934,GO:0016020,GO:0019538,GO:0030054,GO:0031967,GO:0031975,GO:0031976,GO:0031984,GO:0032991,GO:0034357,GO:0034641,GO:0034645,GO:0042221,GO:0042651,GO:0042742,GO:0043043,GO:0043170,GO:0043207,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044391,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044436,GO:0044444,GO:0044446,GO:0044464,GO:0050896,GO:0051704,GO:0051707,GO:0055035,GO:0055044,GO:0071704,GO:0098542,GO:1901564,GO:1901566,GO:1901576,GO:1990904
Exons1
EggNOG free text desc.structural constituent of ribosome
EggNOG OGsCOG0222@1,KOG1715@2759
Cds size393
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01610,ko00000,ko00001,ko00002,ko03011
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842485.1752346-CDS-E_fasciculatus_S2_contig8247:707..11001696842485.1752346-CDS-E_fasciculatus_S2_contig8247:707..1100Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8247 708..1100 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig8247.16394.1prot_E_fasciculatus_S2_contig8247.16394.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig8247 708..1100 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig8247.16394.1

>prot_E_fasciculatus_S2_contig8247.16394.1 ID=prot_E_fasciculatus_S2_contig8247.16394.1|Name=mRNA_E_fasciculatus_S2_contig8247.16394.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=131bp
MTEKISTLIDELKTLTLLEAAELVKAIEETFDVDASAGGGVMMMSGGATT
SEDNGSGEEKTEFDVSLDEVPKDKKISILKIVRSLTELGLKEAKEVVENT
PKVIQEGLTKSAAEEAKKVLEEAGAVVTLK*
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mRNA from alignment at E_fasciculatus_S2_contig8247:708..1100+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig8247.16394.1 ID=mRNA_E_fasciculatus_S2_contig8247.16394.1|Name=mRNA_E_fasciculatus_S2_contig8247.16394.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=393bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8247:708..1100+ (Ectocarpus fasciculatus EfasUO2)
ATGACTGAAAAAATTTCAACTTTAATTGACGAATTAAAAACATTAACATT
ATTAGAAGCTGCTGAATTAGTTAAAGCTATAGAAGAAACTTTTGATGTTG
ATGCTTCTGCTGGTGGCGGTGTTATGATGATGAGTGGAGGTGCAACAACT
TCTGAAGATAATGGAAGTGGGGAAGAGAAAACTGAATTTGATGTCAGCTT
AGATGAAGTCCCTAAAGATAAAAAAATCTCGATCTTAAAAATAGTAAGAT
CTTTAACAGAGTTAGGATTAAAAGAGGCTAAGGAAGTAGTCGAAAATACA
CCAAAAGTTATTCAAGAGGGACTAACTAAATCTGCGGCAGAAGAAGCTAA
AAAAGTCCTTGAAGAGGCTGGTGCTGTTGTAACATTAAAATAA
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig8247:708..1100+

>mRNA_E_fasciculatus_S2_contig8247.16394.1 ID=mRNA_E_fasciculatus_S2_contig8247.16394.1|Name=mRNA_E_fasciculatus_S2_contig8247.16394.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=393bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8247:708..1100+ (Ectocarpus fasciculatus EfasUO2)
ATGACTGAAAAAATTTCAACTTTAATTGACGAATTAAAAACATTAACATT
ATTAGAAGCTGCTGAATTAGTTAAAGCTATAGAAGAAACTTTTGATGTTG
ATGCTTCTGCTGGTGGCGGTGTTATGATGATGAGTGGAGGTGCAACAACT
TCTGAAGATAATGGAAGTGGGGAAGAGAAAACTGAATTTGATGTCAGCTT
AGATGAAGTCCCTAAAGATAAAAAAATCTCGATCTTAAAAATAGTAAGAT
CTTTAACAGAGTTAGGATTAAAAGAGGCTAAGGAAGTAGTCGAAAATACA
CCAAAAGTTATTCAAGAGGGACTAACTAAATCTGCGGCAGAAGAAGCTAA
AAAAGTCCTTGAAGAGGCTGGTGCTGTTGTAACATTAAAATAA
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