mRNA_E_fasciculatus_S2_contig8196.16346.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig8196.16346.1
Unique NamemRNA_E_fasciculatus_S2_contig8196.16346.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin (n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6A7_ECTSI)

HSP 1 Score: 291 bits (744), Expect = 3.380e-99
Identity = 141/149 (94.63%), Postives = 144/149 (96.64%), Query Frame = 1 
Query:    1 GTKRAVQVTLYEEFSRYADRRSVAQEVIFEITSSEHFEDVVSRAAGADEHEGYEDAQGNWRVPHKLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRDNT 447
            G     +VTLYEEFSRYADRRSVAQEVIFEITS+EHFEDVVSRAAGADEHEGYEDAQGNWRVPHKLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAH+EELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRDNT
Sbjct:   18 GAADTAKVTLYEEFSRYADRRSVAQEVIFEITSAEHFEDVVSRAAGADEHEGYEDAQGNWRVPHKLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHHEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRDNT 166
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin (n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3K6_9STRA)

HSP 1 Score: 173 bits (439), Expect = 4.260e-53
Identity = 76/140 (54.29%), Postives = 108/140 (77.14%), Query Frame = 1 
Query:   28 LYEEFSRYADRRSVAQEVIFEITSSEHFEDVVSRAAGADEHE-GYEDAQGNWRVPHKLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRDN 444
            +YEE S+YAD  +VA + + EITS+EH + +++ AA  +  + GY D  GN R+PH+L+M+Q+HA WCR C G++PKLLKL  +HPEVLCCK+NK ++E++AA+LG+R LP  + YKNGKR+DH TTA+I TIE+ + DN
Sbjct:    1 MYEESSKYADPHAVATDCVVEITSNEHLDRILATAAQVENPDAGYVDHHGNQRLPHRLIMIQVHANWCRACIGVRPKLLKLCARHPEVLCCKLNKGNHEDVAARLGIRALPTFVLYKNGKRIDHFTTADIETIEQGVLDN 140
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Hypothetical (protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U8D8_9STRA)

HSP 1 Score: 127 bits (318), Expect = 6.380e-34
Identity = 61/138 (44.20%), Postives = 85/138 (61.59%), Query Frame = 1 
Query:   31 YEEFSRYADRRSVAQEVIFEITSSEHFEDVVSRAAGADEHEGYEDAQGNWRVPHKLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRDN 444
            YEE S YAD  + A E + ++TS E F+ ++S AAGA+            + PHK++M+ +HA WCR CK L  + LK+   HPEVLC K+N A N E+   LG+RG+P+ I YK GKR+DH    N + +EE + DN
Sbjct:   75 YEECSFYADATACAMETLIDVTSQEQFDFIISHAAGAN-------PLSPDQKPHKMVMLMVHATWCRKCKYLMKQYLKVAKAHPEVLCLKLNLATNAEVGKSLGIRGVPLFIIYKAGKRIDHFYAENADDLEEQVEDN 205
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin (n=2 Tax=Polaribacter TaxID=52959 RepID=A0A1B8TUF0_9FLAO)

HSP 1 Score: 62.8 bits (151), Expect = 5.150e-10
Identity = 29/72 (40.28%), Postives = 46/72 (63.89%), Query Frame = 1 
Query:  190 HKLMMVQIHAGWCRVCKGLQPKLLKLITKHPE-VLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHT 402
            +  +++  +A WC  C+ L P + KL  +  + V   K+N  +N+ELAAK G+R +P L+F+K+GK VD HT
Sbjct:   17 NSTVLLDFYADWCGPCQTLLPTIHKLADELKDNVTIKKVNVDNNQELAAKFGIRNIPTLVFFKDGKAVDKHT 88
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin (n=1 Tax=Helicobacter enhydrae TaxID=222136 RepID=A0A1B1U5P4_9HELI)

HSP 1 Score: 61.6 bits (148), Expect = 1.440e-9
Identity = 31/83 (37.35%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:  196 LMMVQIHAGWCRVCKGLQPKLLKLITKHP-EVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRD 441
            + +V   A WC  C+ L P + KL  ++  +   CK+N    EEL+A+ G+R +P ++F+K+GK VD    AN    E+AI+D
Sbjct:   20 VALVDFWAPWCGPCRMLSPVIDKLADEYESKARICKVNTDEQEELSAQFGIRSIPTILFFKDGKVVDQMVGANS---EQAIKD 99
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin (n=1 Tax=Candidatus Woesearchaeota archaeon TaxID=2026803 RepID=A0A7C4UZH4_9ARCH)

HSP 1 Score: 60.1 bits (144), Expect = 5.240e-9
Identity = 30/81 (37.04%), Postives = 43/81 (53.09%), Query Frame = 1 
Query:  202 MVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTT-ANINTIEEAIRD 441
            +V   A WC  CK + P   +    H E L  K+N     EL+  LG+RG+P LIF+++GK V  H   A+   +E  I+D
Sbjct:   19 VVDFWAPWCGPCKMMAPVFEETSKTHNEALFAKVNTDEEPELSTSLGIRGIPTLIFFRDGKEVARHVGYADKRVLEAKIKD 99
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin-like (chloroplastic-like n=1 Tax=Tetraselmis sp. GSL018 TaxID=582737 RepID=A0A061S1S4_9CHLO)

HSP 1 Score: 62.0 bits (149), Expect = 7.440e-9
Identity = 31/84 (36.90%), Postives = 49/84 (58.33%), Query Frame = 1 
Query:  193 KLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHNEELAAKLGVRGLPMLIFYK-NGKRVDHHTTA--NINTIEEAI 435
            +L++V  +A WC  C+GL P+L KL+ +HP+V   K+    N  L   LGV+ LP   FY+ N  R+D  + +   I  +++AI
Sbjct:   99 RLVIVDFYATWCHACRGLYPRLSKLMEEHPDVKLLKVEFESNRSLCKSLGVKVLPFFHFYRGNEGRLDAFSASLKKIGRLKDAI 182
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: thioredoxin (n=1 Tax=Francisella sp. SYW-9 TaxID=2610888 RepID=UPI00123E3EB0)

HSP 1 Score: 59.7 bits (143), Expect = 8.480e-9
Identity = 27/67 (40.30%), Postives = 41/67 (61.19%), Query Frame = 1 
Query:  193 KLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVD 393
            K ++V  +A WC  CK L P L +L   +   L  K+N   N+ LAA+ G+R +P +I +KNGK+V+
Sbjct:   23 KPVLVDFYADWCGPCKTLGPILDQLSKDYKNALIVKVNVDDNQNLAAQFGIRSIPTMIIFKNGKQVE 89
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Thioredoxin (n=1 Tax=Candidatus Fokinia solitaria TaxID=1802984 RepID=A0A2U8BSN6_9RICK)

HSP 1 Score: 59.7 bits (143), Expect = 9.090e-9
Identity = 28/65 (43.08%), Postives = 39/65 (60.00%), Query Frame = 1 
Query:  199 MMVQIHAGWCRVCKGLQPKLLKLITKHP-EVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRV 390
            ++V   A WC  CK L P L  L   +   V  CK+N   N E+ A +GVRG+PML+ YK+GK++
Sbjct:   24 VLVDFWAPWCGPCKALSPVLESLAKSYEGRVKVCKMNVDENTEIVASVGVRGIPMLVLYKDGKQI 88
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BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Match: Hypothetical (protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A6U4NA76_HEMAN)

HSP 1 Score: 61.6 bits (148), Expect = 1.470e-8
Identity = 25/84 (29.76%), Postives = 50/84 (59.52%), Query Frame = 1 
Query:  193 KLMMVQIHAGWCRVCKGLQPKLLKLITKHP-EVLCCKINKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRD 441
            ++++++++A WCR C  +  ++ +   ++  E +  K++     +L+ KLGVRG+P  I YK+G+R+DH    N   + E + D
Sbjct:  157 RIVILEVYANWCRKCLKMSKEVFRACNQYQNEAIFLKMDAKACPDLSKKLGVRGMPTFIVYKDGERIDHFNAGNREDLNEHLED 240
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig8196.16346.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Thioredoxin3.380e-9994.63n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7... [more]
Thioredoxin4.260e-5354.29n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z... [more]
Hypothetical6.380e-3444.20protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=1... [more]
Thioredoxin5.150e-1040.28n=2 Tax=Polaribacter TaxID=52959 RepID=A0A1B8TUF0_... [more]
Thioredoxin1.440e-937.35n=1 Tax=Helicobacter enhydrae TaxID=222136 RepID=A... [more]
Thioredoxin5.240e-937.04n=1 Tax=Candidatus Woesearchaeota archaeon TaxID=2... [more]
Thioredoxin-like7.440e-936.90chloroplastic-like n=1 Tax=Tetraselmis sp. GSL018 ... [more]
thioredoxin8.480e-940.30n=1 Tax=Francisella sp. SYW-9 TaxID=2610888 RepID=... [more]
Thioredoxin9.090e-943.08n=1 Tax=Candidatus Fokinia solitaria TaxID=1802984... [more]
Hypothetical1.470e-829.76protein n=1 Tax=Hemiselmis andersenii TaxID=464988... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig8196contigE_fasciculatus_S2_contig8196:4590..6376 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score294.7
Seed ortholog evalue2.5e-77
Seed eggNOG ortholog2880.D7G6A7
Model size450
KEGG koko:K03671
KEGG Pathwayko04621,ko05418,map04621,map05418
Hectar predicted targeting categoryother localisation
Exons3
EggNOG free text desc.protein-disulfide reductase activity
EggNOG OGsKOG0908@1,KOG0908@2759
Ec32 ortholog descriptionThioredoxin-like fold
Ec32 orthologEc-10_001160.1
Cds size450
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03110
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842480.5900857-CDS-E_fasciculatus_S2_contig8196:4589..47181696842480.5900857-CDS-E_fasciculatus_S2_contig8196:4589..4718Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8196 4590..4718 -
1696842480.6053174-CDS-E_fasciculatus_S2_contig8196:4967..51981696842480.6053174-CDS-E_fasciculatus_S2_contig8196:4967..5198Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8196 4968..5198 -
1696842480.61526-CDS-E_fasciculatus_S2_contig8196:6286..63761696842480.61526-CDS-E_fasciculatus_S2_contig8196:6286..6376Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8196 6287..6376 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig8196.16346.1prot_E_fasciculatus_S2_contig8196.16346.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig8196 4590..6376 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig8196.16346.1

>prot_E_fasciculatus_S2_contig8196.16346.1 ID=prot_E_fasciculatus_S2_contig8196.16346.1|Name=mRNA_E_fasciculatus_S2_contig8196.16346.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=150bp
GTKRAVQVTLYEEFSRYADRRSVAQEVIFEITSSEHFEDVVSRAAGADEH
EGYEDAQGNWRVPHKLMMVQIHAGWCRVCKGLQPKLLKLITKHPEVLCCK
INKAHNEELAAKLGVRGLPMLIFYKNGKRVDHHTTANINTIEEAIRDNT*
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mRNA from alignment at E_fasciculatus_S2_contig8196:4590..6376-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig8196.16346.1 ID=mRNA_E_fasciculatus_S2_contig8196.16346.1|Name=mRNA_E_fasciculatus_S2_contig8196.16346.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=1787bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8196:4590..6376- (Ectocarpus fasciculatus EfasUO2)
GGTACCAAACGGGCAGTGCAGGTGACCCTCTACGAAGAGTTCTCCAGGTA
CGCAGACAGAAGATCTGTGGCACAGGAGGTCATCTTCGAGGTGAGTGGGA
CTTTTCGTTGCACTCGCTTGTCACAAACTGTTAAAATACTGCTGTGTGTA
CTAGCTGCATCGTGATGGGACATGGTGCCACACTCCTCCCGCCGGTCGAG
TGTTGGTACTGCTGTAGTCCTGCATGCGCTCTCTCGGCGGATATCAACAG
GTACGTGCGCCTATCATGTTCTTGTACTCCATCCATTCCCGTGACTGTTG
GATGTTGGGCAAAAGGTGCATTGTATCCGTATGACATGGTAGCCCGGGGT
TGGCATTTGGTTTGTTGCTCCACCGCGAGTCTGTGGAAATCCGCAACACT
GTTTTTTGGAGAATCGCCCACCTCTCAAGAAATAAATTGCCCTGCTCACG
CAGGGCTGCCCCCAACCCGAGGTCCGCTTTTTCCTTTGAAACGTGCGTGG
TTGGTTGCAACGAAGTGTTCTCCAAATCACATGAAGACTTGCGTTGCGCG
AACAGGCGGCGGGCGGCTTTTGCCGAATTCCTTGCGGAAGTTGGGAACAG
AATTGGATGCGTAAGGATAATCGTTCAATCCGTAAACGAGCCAAAAAAGC
AGCCACGTATCTTCCATTGCTTGGGCCGTGTGCTACTACTTCTTGTACAT
ATGTGCAGGATGTGCATGCACAACAACAGCAGAAACAATACAAGCGCACA
TCGAACGCGATCGCGCTGGTGAGAACACTTCCAAACATGCTAGCCGGTGC
TCACTTCTTTTCATGTCTCTCAACAGGGTCTTCAATGCTCAGTAAGGTTA
AAAATAGGCCAGCGTTTCGCAGTCGGAGGGAGGGCGGGGCGAAAGCGGGA
AATCGAACACCAGAGAATCGATTACAGGGTAGAGGGACAGCCGAAGGCAC
ACGCTCGAATAGTACATGTACACGCACATGCAGGAGAAGCTTTCGTCTCC
TCCGATGAAACTCTAGGTTCCAGTGTGCCTGAATTTCCGAGTGAACTCCG
GGGGGGTAGTGTGCCCAAATCGGGCATGTCGGCGACACGAAGGCCCACAA
GTGTGTGTTGAGGTCTCCCTCAAGCAAACGCTGGAGATGCATGCCATGAC
AAGCGAACCCACCGCTCTGTCCATGCAGATTACTTCATCGGAGCATTTTG
AGGACGTGGTATCTAGGGCAGCAGGAGCGGATGAACATGAGGGGTACGAG
GACGCTCAAGGAAACTGGAGAGTTCCACACAAACTCATGATGGTCCAAAT
TCATGCCGGCTGGTGCCGTGTTTGCAAGGGACTCCAACCAAAATTGCTGA
AGCTCATCACCAAGCACCCTGAGGTTCTCTGCTGCAAGATCAACAAGGCA
CACAACGAAGTGAGTGGGTCTGATGGGATCCATATCGCGTAGAACTGCAC
GATCCACATGGCGTAGTACTGCACAATGCCAGAAAGTGCAGGAAGTTGCC
TTTCCCCAAGTGAACCGAATGGGGTCATTCGCTGAATCCGGACGATACGT
GACATGGTAACAATCGAAGCAAGGTCGACCGCCAACTGCTTTATTTTTCT
TTTCTCACTATAATTTCCGACTGCTGACGCTTTGCATGCTGTTGCCACAA
TCTCGCAGGAGCTTGCGGCAAAGCTGGGTGTGCGTGGGCTGCCGATGCTC
ATCTTTTACAAGAACGGAAAGCGGGTGGATCACCACACAACTGCGAACAT
CAACACAATAGAGGAGGCCATCAGAGATAATACTTGA
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig8196:4590..6376-

>mRNA_E_fasciculatus_S2_contig8196.16346.1 ID=mRNA_E_fasciculatus_S2_contig8196.16346.1|Name=mRNA_E_fasciculatus_S2_contig8196.16346.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=450bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8196:4590..6376- (Ectocarpus fasciculatus EfasUO2)
GGTACCAAACGGGCAGTGCAGGTGACCCTCTACGAAGAGTTCTCCAGGTA
CGCAGACAGAAGATCTGTGGCACAGGAGGTCATCTTCGAGATTACTTCAT
CGGAGCATTTTGAGGACGTGGTATCTAGGGCAGCAGGAGCGGATGAACAT
GAGGGGTACGAGGACGCTCAAGGAAACTGGAGAGTTCCACACAAACTCAT
GATGGTCCAAATTCATGCCGGCTGGTGCCGTGTTTGCAAGGGACTCCAAC
CAAAATTGCTGAAGCTCATCACCAAGCACCCTGAGGTTCTCTGCTGCAAG
ATCAACAAGGCACACAACGAAGAGCTTGCGGCAAAGCTGGGTGTGCGTGG
GCTGCCGATGCTCATCTTTTACAAGAACGGAAAGCGGGTGGATCACCACA
CAACTGCGAACATCAACACAATAGAGGAGGCCATCAGAGATAATACTTGA
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