mRNA_E_fasciculatus_S2_contig7942.16102.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig7942.16102.1
Unique NamemRNA_E_fasciculatus_S2_contig7942.16102.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: WD-40 (repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LB83_ECTSI)

HSP 1 Score: 177 bits (450), Expect = 8.890e-50
Identity = 87/89 (97.75%), Postives = 89/89 (100.00%), Query Frame = 1 
Query:   16 RSLRPKGVMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEV 282
            RSLRPKGV+ELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEE+
Sbjct:  605 RSLRPKGVLELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEL 693
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: UDENN (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KB05_9PHAE)

HSP 1 Score: 174 bits (440), Expect = 1.980e-48
Identity = 85/88 (96.59%), Postives = 87/88 (98.86%), Query Frame = 1 
Query:   19 SLRPKGVMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEV 282
            SLRPKGV+ELFAA+LQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDAL EV
Sbjct:  633 SLRPKGVLELFAAVLQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALGEV 720
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RME7_9STRA)

HSP 1 Score: 95.1 bits (235), Expect = 8.850e-21
Identity = 43/81 (53.09%), Postives = 59/81 (72.84%), Query Frame = 1 
Query:   40 MELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEV 282
            +EL +  L E ++L HSA  A LA V E V  LMYPLQW + YVPVLP  ++E +ESPQPFI+G+H+DWL ++ P+ LE++
Sbjct:  283 VELLSYALGERKLLFHSADSAALAPVTEAVCTLMYPLQWIHTYVPVLPRSMLEVLESPQPFIIGIHSDWLPEVRPEVLEDI 363
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: UDENN (domain-containing protein n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JCP4_FISSO)

HSP 1 Score: 79.0 bits (193), Expect = 4.330e-15
Identity = 42/92 (45.65%), Postives = 58/92 (63.04%), Query Frame = 1 
Query:    7 SIRRSLRPKGVMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEV 282
            ++ R L   G+  L AA+L E +ILLHS   A L  VAE V AL+YP QW   YVPVLP  ++E +E+P P++LG+ +  +  I P  LE+V
Sbjct:  994 TVCRLLGADGLTTLLAALLTESKILLHSDDVANLCLVAEVVTALIYPFQWSLPYVPVLPAAMMEFIEAPMPYLLGIPSCNMKHIDPSVLEDV 1085
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: UDENN (domain-containing protein (Fragment) n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A3R6YZQ8_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 4.960e-14
Identity = 34/66 (51.52%), Postives = 49/66 (74.24%), Query Frame = 1 
Query:   61 LQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDI 258
            L E RI++HS + +LL  + E + ALMYP +WP+ YVP+LP  L E+V++P P+ILGVH+ WLS +
Sbjct:  292 LCEYRIVVHSTQLSLLCPITETIKALMYPFRWPHPYVPMLPRILSEYVQAPLPYILGVHSAWLSSL 357
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: Uncharacterized (protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TW42_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 5.180e-14
Identity = 34/66 (51.52%), Postives = 49/66 (74.24%), Query Frame = 1 
Query:   61 LQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDI 258
            L E RI++HS + +LL  + E + ALMYP +WP+ YVP+LP  L E+V++P P+ILGVH+ WLS +
Sbjct:  524 LCEYRIVVHSTQLSLLCPITETIKALMYPFRWPHPYVPMLPRILSEYVQAPLPYILGVHSAWLSSL 589
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: DENN (domain-containing protein 4C n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GQK2_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 5.210e-14
Identity = 40/94 (42.55%), Postives = 61/94 (64.89%), Query Frame = 1 
Query:    7 SIRRSLRPKGVMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDI---APDALEE 279
            ++ R L PK  ++LFA IL E +I+LHS   ++L  VAE + +LM+PL W   Y+PV PN L   + +P+PF++G+ TD  + +   AP+A EE
Sbjct:  586 TVFRCLGPKEFVQLFALILLEKKIILHSRYVSVLNQVAEAMRSLMFPLNWQNIYIPVCPNNLAHFLAAPRPFVIGLQTDAFAQVQLSAPEAGEE 679
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2PC09_9STRA)

HSP 1 Score: 70.1 bits (170), Expect = 1.800e-13
Identity = 37/83 (44.58%), Postives = 53/83 (63.86%), Query Frame = 1 
Query:   34 GVMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEV 282
            G++ + AA+L E ++LLHS   + LA V E V AL+YP  W   YVPVLP  ++E VE+P  +I+GV T  ++ I  D L +V
Sbjct:    2 GLVTILAAVLTESKVLLHSNDSSNLAMVGEVVNALIYPFVWALPYVPVLPREMLEFVEAPLSYIIGVPTCMMNSINADQLTDV 84
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: UDENN (domain-containing protein n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024G4E6_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 1.810e-13
Identity = 31/71 (43.66%), Postives = 51/71 (71.83%), Query Frame = 1 
Query:   37 VMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWL 249
            ++++ A  L E RI++HS   A+L  VAE +  L+YP +W + YVPVLP  L++++++P P+ILG+H+ WL
Sbjct:  581 IIDIVALTLLEYRIVIHSTNMAVLCPVAEAICTLLYPFRWQHPYVPVLPRYLLDYLQAPLPYILGLHSSWL 651
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BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Match: Uncharacterized (protein AlNc14C183G8266 n=1 Tax=Albugo laibachii Nc14 TaxID=890382 RepID=F0W1U9_9STRA)

HSP 1 Score: 72.0 bits (175), Expect = 1.170e-12
Identity = 30/71 (42.25%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:   37 VMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPLQWPYAYVPVLPNCLIEHVESPQPFILGVHTDWL 249
            ++ +    L E RI++HS+  A+L  VAE +  L+YP +W + YVPVLP  L++++++P P+ILG+H+ WL
Sbjct:  608 IINIMTLTLLEYRIVIHSSNMAVLCPVAEALCTLLYPFRWQHPYVPVLPRYLLDYLQAPLPYILGLHSSWL 678
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig7942.16102.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
WD-408.890e-5097.75repeat protein n=1 Tax=Ectocarpus siliculosus TaxI... [more]
UDENN1.980e-4896.59domain-containing protein n=1 Tax=Ectocarpus sp. C... [more]
Hypothetical8.850e-2153.09protein (Fragment) n=1 Tax=Rhizochromulina marina ... [more]
UDENN4.330e-1545.65domain-containing protein n=2 Tax=Fistulifera sola... [more]
UDENN4.960e-1451.52domain-containing protein (Fragment) n=1 Tax=Aphan... [more]
Uncharacterized5.180e-1451.52protein n=1 Tax=Aphanomyces invadans TaxID=157072 ... [more]
DENN5.210e-1442.55domain-containing protein 4C n=1 Tax=Hondaea ferme... [more]
Hypothetical1.800e-1344.58protein (Fragment) n=1 Tax=Leptocylindrus danicus ... [more]
UDENN1.810e-1343.66domain-containing protein n=1 Tax=Albugo candida T... [more]
Uncharacterized1.170e-1242.25protein AlNc14C183G8266 n=1 Tax=Albugo laibachii N... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig7942contigE_fasciculatus_S2_contig7942:6038..6725 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score181.8
Seed ortholog evalue1.5e-43
Seed eggNOG ortholog2880.D8LB83
Preferred nameSCD1
Model size282
KEGG koko:K03361,ko:K20164
KEGG Pathwayko04111,ko04120,map04111,map04120
KEGG ModuleM00407
Hectar predicted targeting categoryother localisation
GOsGO:0000151,GO:0000278,GO:0000281,GO:0000819,GO:0000910,GO:0000911,GO:0001568,GO:0001570,GO:0001932,GO:0001934,GO:0001944,GO:0002682,GO:0002683,GO:0002761,GO:0002762,GO:0003674,GO:0003700,GO:0003824,GO:0004842,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005730,GO:0005737,GO:0005739,GO:0005783,GO:0005794,GO:0005886,GO:0006275,GO:0006355,GO:0006464,GO:0006508,GO:0006511,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007049,GO:0007059,GO:0007062,GO:0007154,GO:0007165,GO:0007166,GO:0007176,GO:0007219,GO:0007275,GO:0008150,GO:0008152,GO:0008156,GO:0009056,GO:0009057,GO:0009314,GO:0009411,GO:0009416,GO:0009628,GO:0009653,GO:0009791,GO:0009825,GO:0009888,GO:0009889,GO:0009890,GO:0009892,GO:0009893,GO:0009894,GO:0009896,GO:0009966,GO:0009967,GO:0009987,GO:0010235,GO:0010374,GO:0010440,GO:0010468,GO:0010469,GO:0010498,GO:0010506,GO:0010556,GO:0010558,GO:0010562,GO:0010564,GO:0010594,GO:0010604,GO:0010605,GO:0010632,GO:0010638,GO:0010646,GO:0010647,GO:0010721,GO:0010821,GO:0010822,GO:0010941,GO:0010942,GO:0010948,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0016049,GO:0016567,GO:0016740,GO:0019005,GO:0019219,GO:0019220,GO:0019222,GO:0019538,GO:0019787,GO:0019899,GO:0019941,GO:0022402,GO:0022603,GO:0023051,GO:0023052,GO:0023056,GO:0030135,GO:0030136,GO:0030154,GO:0030162,GO:0030163,GO:0030234,GO:0030323,GO:0030324,GO:0030332,GO:0030334,GO:0030674,GO:0031146,GO:0031224,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031327,GO:0031329,GO:0031331,GO:0031347,GO:0031348,GO:0031396,GO:0031398,GO:0031399,GO:0031401,GO:0031410,GO:0031461,GO:0031625,GO:0031647,GO:0031648,GO:0031974,GO:0031981,GO:0031982,GO:0032268,GO:0032270,GO:0032386,GO:0032388,GO:0032446,GO:0032501,GO:0032502,GO:0032506,GO:0032875,GO:0032876,GO:0032879,GO:0032880,GO:0032991,GO:0033043,GO:0033157,GO:0033554,GO:0033674,GO:0034644,GO:0035239,GO:0035295,GO:0036211,GO:0040007,GO:0040012,GO:0042058,GO:0042176,GO:0042325,GO:0042327,GO:0042802,GO:0042981,GO:0043065,GO:0043067,GO:0043068,GO:0043085,GO:0043161,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043392,GO:0043408,GO:0043410,GO:0043412,GO:0043523,GO:0043525,GO:0043535,GO:0043549,GO:0043632,GO:0044092,GO:0044093,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044389,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0045088,GO:0045309,GO:0045595,GO:0045596,GO:0045637,GO:0045638,GO:0045670,GO:0045671,GO:0045732,GO:0045741,GO:0045742,GO:0045765,GO:0045786,GO:0045824,GO:0045859,GO:0045860,GO:0045862,GO:0045934,GO:0045937,GO:0048471,GO:0048513,GO:0048514,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048731,GO:0048856,GO:0048869,GO:0050730,GO:0050731,GO:0050776,GO:0050777,GO:0050789,GO:0050790,GO:0050793,GO:0050794,GO:0050816,GO:0050821,GO:0050896,GO:0051049,GO:0051050,GO:0051052,GO:0051053,GO:0051093,GO:0051098,GO:0051100,GO:0051101,GO:0051128,GO:0051130,GO:0051171,GO:0051172,GO:0051173,GO:0051174,GO:0051219,GO:0051222,GO:0051223,GO:0051239,GO:0051241,GO:0051246,GO:0051247,GO:0051252,GO:0051270,GO:0051276,GO:0051301,GO:0051338,GO:0051347,GO:0051438,GO:0051443,GO:0051603,GO:0051716,GO:0051726,GO:0055106,GO:0060090,GO:0060255,GO:0060284,GO:0060341,GO:0060541,GO:0061097,GO:0061098,GO:0061136,GO:0061640,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070201,GO:0070372,GO:0070374,GO:0070647,GO:0071214,GO:0071478,GO:0071482,GO:0071704,GO:0071840,GO:0071944,GO:0072358,GO:0072359,GO:0080008,GO:0080090,GO:0080134,GO:0080135,GO:0090049,GO:0090087,GO:0090316,GO:0090329,GO:0090558,GO:0097027,GO:0097708,GO:0098772,GO:0098813,GO:0104004,GO:0140096,GO:0140110,GO:1900407,GO:1900409,GO:1901184,GO:1901186,GO:1901214,GO:1901216,GO:1901342,GO:1901564,GO:1901565,GO:1901575,GO:1901800,GO:1902105,GO:1902106,GO:1902175,GO:1902177,GO:1902410,GO:1902494,GO:1902531,GO:1902533,GO:1902882,GO:1902884,GO:1903010,GO:1903011,GO:1903025,GO:1903026,GO:1903047,GO:1903050,GO:1903052,GO:1903146,GO:1903201,GO:1903203,GO:1903209,GO:1903214,GO:1903223,GO:1903320,GO:1903322,GO:1903362,GO:1903364,GO:1903376,GO:1903378,GO:1903506,GO:1903533,GO:1903670,GO:1903706,GO:1903707,GO:1903747,GO:1903749,GO:1903827,GO:1903829,GO:1903955,GO:1904951,GO:1990234,GO:1990452,GO:2000026,GO:2000058,GO:2000060,GO:2000104,GO:2000112,GO:2000113,GO:2000145,GO:2000273,GO:2000677,GO:2000678,GO:2001141,GO:2001204,GO:2001205,GO:2001233,GO:2001235,GO:2001242,GO:2001244
Exons2
EggNOG free text desc.Rab guanyl-nucleotide exchange factor activity
EggNOG OGsKOG0274@1,KOG0274@2759,KOG2127@1,KOG2127@2759
Cds size282
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko04121,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842454.796579-CDS-E_fasciculatus_S2_contig7942:6037..62221696842454.796579-CDS-E_fasciculatus_S2_contig7942:6037..6222Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7942 6038..6222 +
1696842454.8099856-CDS-E_fasciculatus_S2_contig7942:6628..67251696842454.8099856-CDS-E_fasciculatus_S2_contig7942:6628..6725Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7942 6629..6725 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig7942.16102.1prot_E_fasciculatus_S2_contig7942.16102.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig7942 6038..6725 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig7942.16102.1

>prot_E_fasciculatus_S2_contig7942.16102.1 ID=prot_E_fasciculatus_S2_contig7942.16102.1|Name=mRNA_E_fasciculatus_S2_contig7942.16102.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=94bp
FFSIRRSLRPKGVMELFAAILQEGRILLHSAKPALLAAVAEGVFALMYPL
QWPYAYVPVLPNCLIEHVESPQPFILGVHTDWLSDIAPDALEEV
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mRNA from alignment at E_fasciculatus_S2_contig7942:6038..6725+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig7942.16102.1 ID=mRNA_E_fasciculatus_S2_contig7942.16102.1|Name=mRNA_E_fasciculatus_S2_contig7942.16102.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=688bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7942:6038..6725+ (Ectocarpus fasciculatus EfasUO2)
TTTTTTTCAATCCGCAGATCTCTCCGTCCGAAGGGAGTTATGGAGCTCTT
CGCTGCGATACTGCAGGAGGGTCGCATCCTGCTGCACTCCGCGAAGCCGG
CGCTGTTGGCGGCGGTGGCGGAGGGTGTGTTCGCGCTCATGTACCCGTTG
CAGTGGCCGTACGCTTACGTGCCCGTTCTGCCCAAGTGAGTCGTTGCTTG
ATGTCGTGATTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTATT
GTTGTTGTTGATGATGATGTCGTCACCACTGTTGTTGTTGGTATTGTTGC
TGCCGCTGTTACTGCTGCCGTGGTGGTGTCGCATACGTTTGGCCAGTGTG
GTGTAGCCGCTCATGTTTTTGTGGTACAGCAGTACAACCATACACTGCTT
GCTGTGGTTGTTGTTCAATCTGCCCCGTCGGTTTTTGATCGGCGTGAGGT
GGAAGCTCAGCCCGAAGCCATTAACCCCTGGTGTCCTTGTTCGATCCGCG
TATTGAGCACCGGCGTTTCCTCACGCTTGAAACGCCCTGAATTAACCCCC
TCCTCTCGTTCGCGTTTTTGTGCACACGGACGACACCGCAGTTGCCTGAT
TGAACACGTGGAGTCACCGCAGCCGTTCATCCTCGGCGTTCACACCGATT
GGCTCTCGGACATCGCCCCGGACGCTCTGGAAGAGGTA
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7942:6038..6725+

>mRNA_E_fasciculatus_S2_contig7942.16102.1 ID=mRNA_E_fasciculatus_S2_contig7942.16102.1|Name=mRNA_E_fasciculatus_S2_contig7942.16102.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=282bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7942:6038..6725+ (Ectocarpus fasciculatus EfasUO2)
TTTTTTTCAATCCGCAGATCTCTCCGTCCGAAGGGAGTTATGGAGCTCTT
CGCTGCGATACTGCAGGAGGGTCGCATCCTGCTGCACTCCGCGAAGCCGG
CGCTGTTGGCGGCGGTGGCGGAGGGTGTGTTCGCGCTCATGTACCCGTTG
CAGTGGCCGTACGCTTACGTGCCCGTTCTGCCCAATTGCCTGATTGAACA
CGTGGAGTCACCGCAGCCGTTCATCCTCGGCGTTCACACCGATTGGCTCT
CGGACATCGCCCCGGACGCTCTGGAAGAGGTA
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