mRNA_E_fasciculatus_S2_contig7698.15864.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig7698.15864.1
Unique NamemRNA_E_fasciculatus_S2_contig7698.15864.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: Putative (homoaconitate hydratase, c-terminal part n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5W8_ECTSI)

HSP 1 Score: 85.1 bits (209), Expect = 8.500e-15
Identity = 37/50 (74.00%), Postives = 46/50 (92.00%), Query Frame = 2 
Query:  389 QDAGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDLLTEGSSVQ 538
            +DAGIYLASPYTAAASA+TGYVTDPREVF FD+ KRW++ED+LT+ + V+
Sbjct:  227 KDAGIYLASPYTAAASAITGYVTDPREVFAFDQDKRWAIEDILTDSARVE 276
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KHV3_9PHAE)

HSP 1 Score: 85.5 bits (210), Expect = 4.120e-14
Identity = 37/50 (74.00%), Postives = 46/50 (92.00%), Query Frame = 2 
Query:  389 QDAGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDLLTEGSSVQ 538
            +DAGIYLASPYTAAASA+TGYVTDPREVF FD+ KRW++ED+LT+ + V+
Sbjct:  484 KDAGIYLASPYTAAASAITGYVTDPREVFGFDQDKRWAIEDILTDSARVE 533
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=1 Tax=Scytosiphon lomentaria TaxID=27967 RepID=X2KY86_SCYLO)

HSP 1 Score: 82.4 bits (202), Expect = 4.250e-13
Identity = 37/47 (78.72%), Postives = 43/47 (91.49%), Query Frame = 2 
Query:  395 AGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDLLTEGSSV 535
            AGIYLASPYTAAASA+TG+VTDPREVF+FDK KRW+VED+L+ G  V
Sbjct:  505 AGIYLASPYTAAASAITGFVTDPREVFEFDKGKRWAVEDILSAGLEV 551
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVF1_9STRA)

HSP 1 Score: 71.2 bits (173), Expect = 1.360e-9
Identity = 34/43 (79.07%), Postives = 39/43 (90.70%), Query Frame = 2 
Query:  389 QDAGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDLL 517
            +DAGI+LASPYTAAASAVTGYVTDPREV+ FDK++R  VE LL
Sbjct:  432 KDAGIFLASPYTAAASAVTGYVTDPREVYGFDKARRHPVETLL 474
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YMP4_PORPP)

HSP 1 Score: 68.6 bits (166), Expect = 1.080e-8
Identity = 31/43 (72.09%), Postives = 37/43 (86.05%), Query Frame = 2 
Query:  389 QDAGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDLL 517
            +D+ +YLASPYTAAASAVTGYVTDPREVF+FDK KR + E  +
Sbjct:  473 KDSSVYLASPYTAAASAVTGYVTDPREVFEFDKQKRVTAEQAI 515
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3INF8_9FLOR)

HSP 1 Score: 64.3 bits (155), Expect = 2.640e-7
Identity = 31/38 (81.58%), Postives = 33/38 (86.84%), Query Frame = 2 
Query:  401 IYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDL 514
            IYLASPYTAAASAVTGYVTDPREV+DFD S+R   E L
Sbjct:  544 IYLASPYTAAASAVTGYVTDPREVYDFDMSERHPTETL 581
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QD35_CHOCR)

HSP 1 Score: 63.9 bits (154), Expect = 3.100e-7
Identity = 30/43 (69.77%), Postives = 35/43 (81.40%), Query Frame = 2 
Query:  386 EQDAGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDL 514
            +++  IYLASPYT AASAVTGYVTDPREV+DFD +KR   E L
Sbjct:  456 DKEGQIYLASPYTTAASAVTGYVTDPREVYDFDANKRHPTETL 498
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BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Match: 3-isopropylmalate (dehydratase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TZ20_9STRA)

HSP 1 Score: 63.2 bits (152), Expect = 5.610e-7
Identity = 31/45 (68.89%), Postives = 39/45 (86.67%), Query Frame = 2 
Query:  389 QDAGIYLASPYTAAASAVTGYVTDPREVFDFDKSKRWSVEDLLTE 523
            +DAGI+LASPYTAAASAVTGYVTDPREVF+F ++++   E L T+
Sbjct:  466 KDAGIFLASPYTAAASAVTGYVTDPREVFEFSQTRQ-PAETLATK 509
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig7698.15864.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
Putative8.500e-1574.00homoaconitate hydratase, c-terminal part n=1 Tax=E... [more]
3-isopropylmalate4.120e-1474.00dehydratase n=1 Tax=Ectocarpus sp. CCAP 1310/34 Ta... [more]
3-isopropylmalate4.250e-1378.72dehydratase n=1 Tax=Scytosiphon lomentaria TaxID=2... [more]
3-isopropylmalate1.360e-979.07dehydratase n=1 Tax=Tribonema minus TaxID=303371 R... [more]
3-isopropylmalate1.080e-872.09dehydratase n=1 Tax=Porphyridium purpureum TaxID=3... [more]
3-isopropylmalate2.640e-781.58dehydratase n=1 Tax=Gracilariopsis chorda TaxID=44... [more]
3-isopropylmalate3.100e-769.77dehydratase n=1 Tax=Chondrus crispus TaxID=2769 Re... [more]
3-isopropylmalate5.610e-768.89dehydratase n=2 Tax=Monodopsidaceae TaxID=425072 R... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig7698contigE_fasciculatus_S2_contig7698:5591..6997 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score85.5
Seed ortholog evalue1.3e-14
Seed eggNOG ortholog2880.D7G5W8
Preferred nameLEU1
Model size1407
KEGG rclassRC00497,RC00976,RC00977,RC01041,RC01046,RC03072
KEGG koko:K00749,ko:K01702,ko:K01703
KEGG ReactionR03896,R03898,R03968,R04001,R08620,R08624,R08628,R08634,R08641,R08645,R10170
KEGG Pathwayko00290,ko00660,ko00966,ko01100,ko01110,ko01210,ko01230,map00290,map00660,map00966,map01100,map01110,map01210,map01230
KEGG ModuleM00432,M00535
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0003861,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006520,GO:0006551,GO:0006790,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009081,GO:0009082,GO:0009098,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009987,GO:0010035,GO:0010038,GO:0010494,GO:0016053,GO:0016143,GO:0016144,GO:0016829,GO:0016835,GO:0016836,GO:0016853,GO:0016866,GO:0019748,GO:0019752,GO:0019757,GO:0019758,GO:0019760,GO:0019761,GO:0032991,GO:0035770,GO:0036464,GO:0042221,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044272,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0044550,GO:0046394,GO:0046686,GO:0050486,GO:0050896,GO:0071704,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901657,GO:1901659,GO:1990904
Exons1
EggNOG free text desc.3-isopropylmalate dehydratase activity
EggNOG OGsCOG0065@1,KOG0454@2759
Ec32 ortholog descriptionHomoaconitate hydratase
Ec32 orthologEc-13_001950.1
EC2.4.1.183,4.2.1.33,4.2.1.35
Cds size252
COG Functional cat.E
CAZyGT5
BiGG ReactioniMM904.YGL009C,iND750.YGL009C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01601,ko00000,ko00001,ko00002,ko01000,ko01003
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842431.9514766-UTR-E_fasciculatus_S2_contig7698:5590..58851696842431.9514766-UTR-E_fasciculatus_S2_contig7698:5590..5885Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig7698 5591..5885 +
1696842431.9786198-UTR-E_fasciculatus_S2_contig7698:6137..69971696842431.9786198-UTR-E_fasciculatus_S2_contig7698:6137..6997Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig7698 6138..6997 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842431.96713-CDS-E_fasciculatus_S2_contig7698:5885..61371696842431.96713-CDS-E_fasciculatus_S2_contig7698:5885..6137Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7698 5886..6137 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig7698.15864.1prot_E_fasciculatus_S2_contig7698.15864.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig7698 5886..6137 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig7698.15864.1

>prot_E_fasciculatus_S2_contig7698.15864.1 ID=prot_E_fasciculatus_S2_contig7698.15864.1|Name=mRNA_E_fasciculatus_S2_contig7698.15864.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=84bp
MTSTKSLEEAYNLTTPSPLSRSLRTNSMHNEQDAGIYLASPYTAAASAVT
GYVTDPREVFDFDKSKRWSVEDLLTEGSSVQAV*
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mRNA from alignment at E_fasciculatus_S2_contig7698:5591..6997+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig7698.15864.1 ID=mRNA_E_fasciculatus_S2_contig7698.15864.1|Name=mRNA_E_fasciculatus_S2_contig7698.15864.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=1407bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7698:5591..6997+ (Ectocarpus fasciculatus EfasUO2)
CAGGAGCTTCAGACGAGGCCGTGGCTACCCGCGGGCTGTTATTTATGATC
CATCCAACATCAGCTTATGCGGAGAACTTTTTGAAGCACCGTCGTGTCCT
GGTGTGTCTTGCATTTAGAGTGTTGAAATTTGGTCTTAGTTTGAGGGGAA
GTTGTGTGGAGGTAAACTGCATGGTATGTTGGTAGGAGAAAAGTCCAACT
GTGTCATGCATCTAGCTAGTGTGTGATTGCTCGGTGCTCTGTCGGTCGTG
ATCGAACTCGGTCAAAAATCAGGATCGGCCTTCATCACCCCATGCATGAC
GTCGACCAAATCGCTGGAAGAAGCTTACAACCTGACAACCCCGTCGCCTC
TGTCTCGATCCCTTCGAACAAACTCCATGCATAATGAACAGGACGCTGGA
ATCTATTTGGCATCGCCTTACACCGCTGCCGCTTCAGCTGTTACCGGTTA
TGTGACAGACCCTCGTGAGGTGTTTGACTTCGACAAGAGCAAGCGTTGGT
CTGTGGAGGACCTTTTGACGGAGGGGTCGAGTGTGCAGGCCGTGTAATTG
CTCTCGCATAGGGTGCCTGATCCTCGTTGATTTGGCATGTTTTTGTCCAA
GGGATTTGCGTGGCGAGAGATCGGTGCAAGTTCCTTCTTGACATCGAAAT
GTTTTCGACGTTTTCACATGTTTCGTCATCTCTCCGACTGAACTAATGTT
TTTCGCAGTGCTCCCCTGTCATCCCGATACCCCCGAAATTGCCCGAACGG
GTCTCATTAGCCGATCTTGACCTTTGGCGCACGTCTCGCGACATTATGTG
TATGCCTCGGTAGAGCATCGCAAATTATGTCCCCTTGCCAAGTTTCGTTG
ACTCCCGGGCGCAATAGTATACGCCACAAGTGTGTGCAGCGATGTGCGTC
GATGCGCGCTGTTTTTGTCGTCTTCGCCGTCTTCCTGTGTTGATCGCTAT
TTTTTTTTCGCCAACAATTTCTGGGGAAAGTCGAATTTCAAGGATCCGCT
ATGCAGCAGCCAACATCAGCATCATCACTGGCAGCAGTCGATAGATTTTC
CTGTGCATATTGTCTAGTCTACGTCAACATGCGCGACTTACATGGAACCT
GCATTCCACTACCTCCTTGAGAGTTATCACCCGATTCCAGCGTCCATGGA
AGCCATCGCGAAGGACTTTCTTTCGTGGAAGCTGGCACGAGAAACTTACC
CATGGCTGGCTGCCATCGTTCACGTGCTTTCATGGAAAAAAGTTGACAGA
CGCCACCGTCAAGCACTTTTGTTCTGGAAGTTTTCAAACTGAAATACAGA
GGTTGCCTGCCACTCCTGACAAACGCGTCCTCCGTGGAAGTTGCTTTCCG
TGCTTCCATTCTGAAGCCTCGGTTGCGGAAAATCTCAGACCTAATTTCAA
AATTGGC
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7698:5591..6997+

>mRNA_E_fasciculatus_S2_contig7698.15864.1 ID=mRNA_E_fasciculatus_S2_contig7698.15864.1|Name=mRNA_E_fasciculatus_S2_contig7698.15864.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=252bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7698:5591..6997+ (Ectocarpus fasciculatus EfasUO2)
ATGACGTCGACCAAATCGCTGGAAGAAGCTTACAACCTGACAACCCCGTC
GCCTCTGTCTCGATCCCTTCGAACAAACTCCATGCATAATGAACAGGACG
CTGGAATCTATTTGGCATCGCCTTACACCGCTGCCGCTTCAGCTGTTACC
GGTTATGTGACAGACCCTCGTGAGGTGTTTGACTTCGACAAGAGCAAGCG
TTGGTCTGTGGAGGACCTTTTGACGGAGGGGTCGAGTGTGCAGGCCGTGT
AA
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