mRNA_D-mesarthrocarpus_Contig10128.1.1 (mRNA) Discosporangium mesarthrocarpum MT17_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-mesarthrocarpus_Contig10128.1.1
Unique NamemRNA_D-mesarthrocarpus_Contig10128.1.1
TypemRNA
OrganismDiscosporangium mesarthrocarpum MT17_79 (Discosporangium mesarthrocarpum MT17_79)
Homology
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BLAST of mRNA_D-mesarthrocarpus_Contig10128.1.1 vs. uniprot
Match: A0A6H5LBL5_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LBL5_9PHAE)

HSP 1 Score: 99.8 bits (247), Expect = 1.750e-17
Identity = 165/542 (30.44%), Postives = 199/542 (36.72%), Query Frame = 3 
Query:  897 PSFRGIDSLQTIPEDSAGFVLVNR--STLCEHGPGCQCWDWS-EHGPGALQGTTSVGSPSESYLA-AANPYRGPATPARGWGGGGDRGTGAAAGPLFTG------LGSIWHTIRVGGVGLA----RRAPWSRRARGGGGTVGRGSRPARETQQQQLKXXXXXXDVGVAAFSGATCSPSRAGHTRQKH-SPHEPGAPAFSSVRTAGQ------GSPTKEKLS----GTQCVPAGQVQGQGQGPGLGHESRRQHSEPTDVNSRAPTPF-----------------HALHNPGNPSDHNPPLPSASVDGPLAYNIXXXXXXXXGTLTHVPPQAPGAVGGRDMVGRDMVGGAGQHPMVAAPSPDPTLCPSPMATASSYRAVQDSTQQGLGGPVTHAAATSAAALPAASAWGASLRPPPLFAGEEKERNIAQAALERAKVVGQRAILVVTLADEAAQEALALELPSPRHGVGLGAGVLDEGCRGGKRGVGNNGGAGGRDVSGLCGGGGIPVHHDRRRQARLVEALALYMKASGMLRGSLPGVLEACD 2396
            PSFRG DSL TIPEDS GFVLV R  S  CEHGP C+CW WS             V SPS SYLA A+NPY GP   A                           LGSIW+T      G+A    RRA WS  ARG GG            QQ +         V  A   GA+    R       H SPH       S+VR  G+      GSP   + S    G+  V     QG   G  +    R+ ++       R   P+                 H   +P +   H+ P P A  DG  +YN         G L     +  GA   R   G D  GG  Q  +V                                                      SLR               +AA +RA+VVG+RAIL+VTL D+ AQEA+AL L SP           D G R      G   G      +G          H RRR   L EALALY+KA  +L+G+LPG L  C+
Sbjct:  769 PSFRGSDSLLTIPEDSTGFVLVTRPSSVSCEHGPHCECWAWSASXXXXXXXXXXXVRSPSASYLATASNPYGGPGKEAXXXXXXXXXXXXXXXXXXXXXXXXXAFLGSIWNTAVNTFRGVAGAHGRRASWST-ARGRGG------------QQHRASVSPPTAAVSPAHMRGASSPRERRQQXXXXHLSPH-------SNVRKGGRVSGSPLGSPCSGRSSDDSRGSNNVVYTHRQGGDDGGKVAAVMRQANANAAVSAYRTDVPYQQGTGGSSYFQQQQHRHHPTSHPLHHRSHSEP-PRARADG--SYNTHGG-----GVL-----EGSGAELYRGR-GYDGSGGVNQVELVE-----------------------------------------------------SLR--------------VKAAADRAEVVGKRAILLVTLGDKTAQEAMALALESPSADP-------DGGARAAAAAAGRAEGGPAVFAAGAA--------HARRRHDMLTEALALYVKALALLQGALPGALVVCE 1194
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BLAST of mRNA_D-mesarthrocarpus_Contig10128.1.1 vs. uniprot
Match: D8LHF6_ECTSI (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHF6_ECTSI)

HSP 1 Score: 83.2 bits (204), Expect = 9.100e-13
Identity = 83/211 (39.34%), Postives = 95/211 (45.02%), Query Frame = 3 
Query:  897 PSFRGIDSLQTIPEDSAGFVLVNR--STLCEHGPGCQCWDWSEHGPGALQGTTSVGSPSESYLA-AANPYRGPATPARGWGGGGDRGTGAAAGPLFTG--LGSIWHT----IRVGGVGLARRAPWSRRARGGGGTVGRGSRPARETQQQQLKXXXXXXDVGVAAFSGATCSPSRAGHTRQKH-SPHEPGAPAFSSVRTAGQGSPTKEKLSG 1499
            PSFRG DSL TIPEDS GFVLV R  S  CEHG  C+CW WS +  G      +V SPS SYLA A+NPY GP   A                       LGSIW+T     R    G  RRA WS   RG GG            QQ +         V  A   GA+    R    +Q+H SPH   + A   VR +G  SP +   SG
Sbjct:  317 PSFRGSDSLLTIPEDSTGFVLVTRPSSVSCEHGSHCECWAWSANAGGCA---AAVMSPSASYLATASNPYGGPGKEAXXXXXXXXXXXXXXXXXXXXXAFLGSIWNTAVNTFRGVAGGHGRRASWSA-VRGRGG------------QQHRASVSPPTAAVSPAHVRGASSPRER---QQQQHLSPH---SNARKGVRVSG--SPLRSPCSG 503
The following BLAST results are available for this feature:
BLAST of mRNA_D-mesarthrocarpus_Contig10128.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A6H5LBL5_9PHAE1.750e-1730.44Protein kinase domain-containing protein n=1 Tax=E... [more]
D8LHF6_ECTSI9.100e-1339.34Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-mesarthrocarpus_Contig10128contigD-mesarthrocarpus_Contig10128:1268..4140 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Discosporangium mesarthrocarpum MT17_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Model size2873
Hectar predicted targeting categoryother localisation
Exons1
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-11_004260.1
Cds size2868
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789157.553579-UTR-D-mesarthrocarpus_Contig10128:1267..12721680789157.553579-UTR-D-mesarthrocarpus_Contig10128:1267..1272Discosporangium mesarthrocarpum MT17_79UTRD-mesarthrocarpus_Contig10128 1268..1272 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789157.5632591-CDS-D-mesarthrocarpus_Contig10128:1272..41401680789157.5632591-CDS-D-mesarthrocarpus_Contig10128:1272..4140Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig10128 1273..4140 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-mesarthrocarpus_Contig10128.1.1prot_D-mesarthrocarpus_Contig10128.1.1Discosporangium mesarthrocarpum MT17_79polypeptideD-mesarthrocarpus_Contig10128 1273..4140 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-mesarthrocarpus_Contig10128.1.1

>prot_D-mesarthrocarpus_Contig10128.1.1 ID=prot_D-mesarthrocarpus_Contig10128.1.1|Name=mRNA_D-mesarthrocarpus_Contig10128.1.1|organism=Discosporangium mesarthrocarpum MT17_79|type=polypeptide|length=956bp
MVAAADPLLDLGLQFEGVGMGGMGVGNEEAWAWTGSMGGQEVQHFPPPPQ
PPPGVAAAAARTGAGAGAGASPAAVGVNLADMAGGGTGDGAVQGLGGLPR
GNEEQGSVANAASRSMPNRSSSSSNTNKHPWESGGGPPAVQVTTTTTTMT
TAPSSQHALPLHSPSAHPASPEQVFYTGRDGVVLPPLASPARGLARCSPP
QTPSPQDAPRYPSTVAAAGESRAGAGAGGRGRDVSGSLSNPRQASYSPQS
VGQSLATSSPKGVLVPLTGDICDSPGGGAGAGAGAGAGAGGVGIPKYPSF
RGIDSLQTIPEDSAGFVLVNRSTLCEHGPGCQCWDWSEHGPGALQGTTSV
GSPSESYLAAANPYRGPATPARGWGGGGDRGTGAAAGPLFTGLGSIWHTI
RVGGVGLARRAPWSRRARGGGGTVGRGSRPARETQQQQLKDQQQLKDVGV
AAFSGATCSPSRAGHTRQKHSPHEPGAPAFSSVRTAGQGSPTKEKLSGTQ
CVPAGQVQGQGQGPGLGHESRRQHSEPTDVNSRAPTPFHALHNPGNPSDH
NPPLPSASVDGPLAYNIPSPSPCHDGTLTHVPPQAPGAVGGRDMVGRDMV
GGAGQHPMVAAPSPDPTLCPSPMATASSYRAVQDSTQQGLGGPVTHAAAT
SAAALPAASAWGASLRPPPLFAGEEKERNIAQAALERAKVVGQRAILVVT
LADEAAQEALALELPSPRHGVGLGAGVLDEGCRGGKRGVGNNGGAGGRDV
SGLCGGGGIPVHHDRRRQARLVEALALYMKASGMLRGSLPGVLEACDRSE
QGRESTACAPPTSSYPADHSCESRCLSERDAAATSAVAAAAMAVAVGGVG
VAVSLQQGQQPWLPQRQQAHVQHHLQPPPSPPPDQHHTMPSLREQLEWLK
GLFTKTLQRTEQCRLRVLATTTLSGAGAEAGAGAGASAGAAVGTTPGLAQ
KQLQKS
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mRNA from alignment at D-mesarthrocarpus_Contig10128:1268..4140+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-mesarthrocarpus_Contig10128.1.1 ID=mRNA_D-mesarthrocarpus_Contig10128.1.1|Name=mRNA_D-mesarthrocarpus_Contig10128.1.1|organism=Discosporangium mesarthrocarpum MT17_79|type=mRNA|length=2873bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig10128:1268..4140+ (Discosporangium mesarthrocarpum MT17_79)
CAGGGATGGTGGCTGCGGCAGACCCCTTGCTGGATTTGGGTCTTCAGTTT
GAGGGGGTGGGGATGGGGGGGATGGGGGTTGGGAATGAGGAGGCATGGGC
TTGGACTGGTAGTATGGGGGGGCAGGAGGTTCAGCACTTCCCGCCTCCCC
CGCAGCCCCCACCTGGGGTGGCTGCAGCAGCAGCGAGGACTGGGGCAGGG
GCAGGGGCAGGGGCAAGTCCTGCTGCTGTTGGGGTCAACTTGGCGGACAT
GGCAGGAGGGGGGACAGGGGATGGGGCAGTGCAAGGTTTGGGTGGCCTGC
CAAGGGGCAATGAGGAGCAGGGCTCTGTGGCTAACGCTGCCTCCAGATCA
ATGCCCAACAGAAGCAGCAGCAGCAGCAACACAAACAAACACCCATGGGA
GTCAGGGGGAGGGCCACCGGCAGTACAAGTAACAACCACCACGACAACGA
TGACGACGGCGCCATCATCTCAGCACGCCCTGCCACTCCATAGCCCGTCC
GCCCACCCGGCCAGCCCGGAGCAGGTCTTCTACACTGGGAGGGACGGGGT
GGTGCTGCCCCCCCTCGCCTCCCCCGCACGAGGGTTGGCACGGTGTTCCC
CTCCCCAGACCCCTTCACCACAAGACGCACCACGATACCCGTCCACCGTG
GCAGCAGCGGGTGAAAGTCGGGCAGGGGCTGGGGCAGGGGGCAGGGGCAG
AGATGTTTCAGGATCCCTGTCAAACCCACGGCAAGCGTCGTATTCCCCCC
AATCAGTGGGCCAATCCCTGGCAACCTCTTCCCCAAAGGGTGTGCTTGTG
CCCCTTACCGGGGACATTTGTGACAGCCCTGGTGGGGGTGCAGGGGCAGG
GGCAGGGGCTGGAGCTGGGGCTGGAGGGGTGGGCATACCTAAGTACCCCA
GCTTCCGGGGCATTGACAGCCTGCAGACCATCCCGGAGGACTCAGCCGGG
TTCGTCCTCGTCAACCGCTCCACCCTCTGTGAGCATGGGCCTGGGTGCCA
GTGCTGGGACTGGTCCGAGCATGGCCCTGGGGCGCTCCAGGGCACCACTT
CTGTGGGCTCGCCCTCGGAGTCCTACCTGGCAGCAGCCAACCCATACAGG
GGCCCAGCGACACCTGCAAGGGGCTGGGGCGGGGGTGGGGATAGGGGCAC
AGGGGCTGCGGCGGGGCCACTCTTCACAGGCCTTGGGTCCATCTGGCACA
CCATCAGGGTTGGGGGCGTGGGCTTGGCAAGGAGGGCACCATGGTCCCGA
CGGGCCAGGGGAGGTGGGGGGACAGTGGGAAGGGGGAGTCGACCGGCCCG
GGAGACACAGCAGCAGCAGCTGAAAGACCAGCAACAGCTGAAGGACGTGG
GCGTGGCTGCTTTCTCAGGCGCAACTTGCTCACCTTCACGGGCAGGGCAC
ACTAGGCAAAAGCACTCCCCCCATGAACCAGGGGCGCCTGCCTTCTCTTC
TGTCCGGACAGCAGGGCAGGGCTCCCCTACCAAGGAAAAACTGTCTGGCA
CACAGTGCGTGCCAGCAGGGCAGGTGCAGGGGCAAGGGCAGGGGCCAGGA
CTGGGACATGAGTCCCGCCGCCAGCACTCGGAGCCCACTGATGTGAACAG
TCGCGCTCCTACACCCTTCCATGCCCTCCACAACCCCGGAAACCCCAGCG
ACCACAACCCCCCCTTGCCATCCGCATCTGTGGATGGACCCCTGGCCTAC
AATATCCCTTCCCCATCCCCCTGTCACGATGGAACCCTGACCCATGTACC
ACCACAAGCCCCTGGGGCAGTGGGGGGGAGGGACATGGTCGGCAGGGACA
TGGTCGGCGGGGCCGGGCAGCACCCTATGGTTGCAGCACCCAGCCCTGAC
CCCACCCTGTGCCCCAGCCCCATGGCCACTGCTTCCAGCTACAGGGCTGT
ACAGGACTCCACTCAGCAGGGGCTGGGGGGGCCCGTGACGCATGCGGCGG
CTACGTCTGCTGCAGCACTGCCAGCTGCATCGGCTTGGGGGGCGAGTCTG
CGCCCACCACCCCTGTTTGCAGGGGAGGAAAAGGAGAGGAACATAGCCCA
GGCGGCATTGGAGCGGGCAAAGGTGGTAGGCCAGCGTGCCATTTTGGTGG
TGACCCTCGCAGATGAGGCTGCTCAAGAGGCTCTGGCCCTAGAGCTGCCC
TCCCCGCGGCATGGGGTGGGGCTGGGGGCAGGGGTGCTGGACGAGGGCTG
CAGAGGAGGAAAACGAGGCGTTGGCAACAACGGAGGAGCTGGAGGAAGAG
ACGTTTCCGGCCTGTGTGGGGGTGGGGGTATTCCCGTGCACCATGACCGG
CGGCGGCAGGCGAGGCTTGTGGAAGCGCTGGCGCTGTACATGAAGGCCTC
TGGCATGCTGCGGGGCTCCCTGCCTGGTGTCCTTGAGGCCTGCGACAGGT
CCGAGCAAGGCAGGGAGTCCACCGCCTGTGCACCACCCACCTCCTCTTAC
CCTGCAGACCACTCTTGTGAGAGCAGGTGCCTGTCAGAGAGGGATGCAGC
GGCCACATCAGCCGTGGCGGCCGCAGCCATGGCAGTTGCAGTGGGGGGGG
TAGGCGTGGCAGTATCGCTTCAGCAGGGCCAGCAGCCATGGCTACCGCAA
AGGCAACAAGCCCATGTGCAGCACCACCTGCAGCCACCCCCGTCGCCCCC
GCCTGACCAGCACCACACCATGCCCTCGCTCCGGGAGCAGCTGGAGTGGC
TCAAGGGCCTCTTCACCAAGACTCTGCAACGCACAGAGCAGTGCCGGCTC
CGGGTCTTGGCAACCACAACATTGTCCGGTGCGGGGGCAGAGGCAGGGGC
AGGGGCTGGGGCAAGTGCAGGAGCAGCAGTAGGCACTACACCAGGCCTTG
CCCAGAAACAATTGCAGAAATCC
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Coding sequence (CDS) from alignment at D-mesarthrocarpus_Contig10128:1268..4140+

>mRNA_D-mesarthrocarpus_Contig10128.1.1 ID=mRNA_D-mesarthrocarpus_Contig10128.1.1|Name=mRNA_D-mesarthrocarpus_Contig10128.1.1|organism=Discosporangium mesarthrocarpum MT17_79|type=CDS|length=2868bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig10128:1268..4140+ (Discosporangium mesarthrocarpum MT17_79)
ATGGTGGCTGCGGCAGACCCCTTGCTGGATTTGGGTCTTCAGTTTGAGGG
GGTGGGGATGGGGGGGATGGGGGTTGGGAATGAGGAGGCATGGGCTTGGA
CTGGTAGTATGGGGGGGCAGGAGGTTCAGCACTTCCCGCCTCCCCCGCAG
CCCCCACCTGGGGTGGCTGCAGCAGCAGCGAGGACTGGGGCAGGGGCAGG
GGCAGGGGCAAGTCCTGCTGCTGTTGGGGTCAACTTGGCGGACATGGCAG
GAGGGGGGACAGGGGATGGGGCAGTGCAAGGTTTGGGTGGCCTGCCAAGG
GGCAATGAGGAGCAGGGCTCTGTGGCTAACGCTGCCTCCAGATCAATGCC
CAACAGAAGCAGCAGCAGCAGCAACACAAACAAACACCCATGGGAGTCAG
GGGGAGGGCCACCGGCAGTACAAGTAACAACCACCACGACAACGATGACG
ACGGCGCCATCATCTCAGCACGCCCTGCCACTCCATAGCCCGTCCGCCCA
CCCGGCCAGCCCGGAGCAGGTCTTCTACACTGGGAGGGACGGGGTGGTGC
TGCCCCCCCTCGCCTCCCCCGCACGAGGGTTGGCACGGTGTTCCCCTCCC
CAGACCCCTTCACCACAAGACGCACCACGATACCCGTCCACCGTGGCAGC
AGCGGGTGAAAGTCGGGCAGGGGCTGGGGCAGGGGGCAGGGGCAGAGATG
TTTCAGGATCCCTGTCAAACCCACGGCAAGCGTCGTATTCCCCCCAATCA
GTGGGCCAATCCCTGGCAACCTCTTCCCCAAAGGGTGTGCTTGTGCCCCT
TACCGGGGACATTTGTGACAGCCCTGGTGGGGGTGCAGGGGCAGGGGCAG
GGGCTGGAGCTGGGGCTGGAGGGGTGGGCATACCTAAGTACCCCAGCTTC
CGGGGCATTGACAGCCTGCAGACCATCCCGGAGGACTCAGCCGGGTTCGT
CCTCGTCAACCGCTCCACCCTCTGTGAGCATGGGCCTGGGTGCCAGTGCT
GGGACTGGTCCGAGCATGGCCCTGGGGCGCTCCAGGGCACCACTTCTGTG
GGCTCGCCCTCGGAGTCCTACCTGGCAGCAGCCAACCCATACAGGGGCCC
AGCGACACCTGCAAGGGGCTGGGGCGGGGGTGGGGATAGGGGCACAGGGG
CTGCGGCGGGGCCACTCTTCACAGGCCTTGGGTCCATCTGGCACACCATC
AGGGTTGGGGGCGTGGGCTTGGCAAGGAGGGCACCATGGTCCCGACGGGC
CAGGGGAGGTGGGGGGACAGTGGGAAGGGGGAGTCGACCGGCCCGGGAGA
CACAGCAGCAGCAGCTGAAAGACCAGCAACAGCTGAAGGACGTGGGCGTG
GCTGCTTTCTCAGGCGCAACTTGCTCACCTTCACGGGCAGGGCACACTAG
GCAAAAGCACTCCCCCCATGAACCAGGGGCGCCTGCCTTCTCTTCTGTCC
GGACAGCAGGGCAGGGCTCCCCTACCAAGGAAAAACTGTCTGGCACACAG
TGCGTGCCAGCAGGGCAGGTGCAGGGGCAAGGGCAGGGGCCAGGACTGGG
ACATGAGTCCCGCCGCCAGCACTCGGAGCCCACTGATGTGAACAGTCGCG
CTCCTACACCCTTCCATGCCCTCCACAACCCCGGAAACCCCAGCGACCAC
AACCCCCCCTTGCCATCCGCATCTGTGGATGGACCCCTGGCCTACAATAT
CCCTTCCCCATCCCCCTGTCACGATGGAACCCTGACCCATGTACCACCAC
AAGCCCCTGGGGCAGTGGGGGGGAGGGACATGGTCGGCAGGGACATGGTC
GGCGGGGCCGGGCAGCACCCTATGGTTGCAGCACCCAGCCCTGACCCCAC
CCTGTGCCCCAGCCCCATGGCCACTGCTTCCAGCTACAGGGCTGTACAGG
ACTCCACTCAGCAGGGGCTGGGGGGGCCCGTGACGCATGCGGCGGCTACG
TCTGCTGCAGCACTGCCAGCTGCATCGGCTTGGGGGGCGAGTCTGCGCCC
ACCACCCCTGTTTGCAGGGGAGGAAAAGGAGAGGAACATAGCCCAGGCGG
CATTGGAGCGGGCAAAGGTGGTAGGCCAGCGTGCCATTTTGGTGGTGACC
CTCGCAGATGAGGCTGCTCAAGAGGCTCTGGCCCTAGAGCTGCCCTCCCC
GCGGCATGGGGTGGGGCTGGGGGCAGGGGTGCTGGACGAGGGCTGCAGAG
GAGGAAAACGAGGCGTTGGCAACAACGGAGGAGCTGGAGGAAGAGACGTT
TCCGGCCTGTGTGGGGGTGGGGGTATTCCCGTGCACCATGACCGGCGGCG
GCAGGCGAGGCTTGTGGAAGCGCTGGCGCTGTACATGAAGGCCTCTGGCA
TGCTGCGGGGCTCCCTGCCTGGTGTCCTTGAGGCCTGCGACAGGTCCGAG
CAAGGCAGGGAGTCCACCGCCTGTGCACCACCCACCTCCTCTTACCCTGC
AGACCACTCTTGTGAGAGCAGGTGCCTGTCAGAGAGGGATGCAGCGGCCA
CATCAGCCGTGGCGGCCGCAGCCATGGCAGTTGCAGTGGGGGGGGTAGGC
GTGGCAGTATCGCTTCAGCAGGGCCAGCAGCCATGGCTACCGCAAAGGCA
ACAAGCCCATGTGCAGCACCACCTGCAGCCACCCCCGTCGCCCCCGCCTG
ACCAGCACCACACCATGCCCTCGCTCCGGGAGCAGCTGGAGTGGCTCAAG
GGCCTCTTCACCAAGACTCTGCAACGCACAGAGCAGTGCCGGCTCCGGGT
CTTGGCAACCACAACATTGTCCGGTGCGGGGGCAGAGGCAGGGGCAGGGG
CTGGGGCAAGTGCAGGAGCAGCAGTAGGCACTACACCAGGCCTTGCCCAG
AAACAATTGCAGAAATCC
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