mRNA_D-mesarthrocarpus_Contig1082.6.1 (mRNA) Discosporangium mesarthrocarpum MT17_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-mesarthrocarpus_Contig1082.6.1
Unique NamemRNA_D-mesarthrocarpus_Contig1082.6.1
TypemRNA
OrganismDiscosporangium mesarthrocarpum MT17_79 (Discosporangium mesarthrocarpum MT17_79)
Homology
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: D7G4M1_ECTSI (Smc-like protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G4M1_ECTSI)

HSP 1 Score: 147 bits (371), Expect = 5.680e-39
Identity = 73/101 (72.28%), Postives = 82/101 (81.19%), Query Frame = 1 
Query:    1 SRGGVKGMRGMVTSEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLNTEEGF 303
            S+ G K  R     EAG++LK+ V NFMCHRKLS+ LCKHVNFI+GRNGSGKSAILAALQICLGA+A LTHRA ++ D IRHG KGDA LEVTLLNTE GF
Sbjct:   68 SQRGKKRKRKTEQPEAGVVLKVHVSNFMCHRKLSVPLCKHVNFINGRNGSGKSAILAALQICLGAKAHLTHRAKKMADFIRHGWKGDAVLEVTLLNTEHGF 168
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: D7G4M2_ECTSI (Smc-like protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G4M2_ECTSI)

HSP 1 Score: 143 bits (360), Expect = 1.730e-37
Identity = 69/88 (78.41%), Postives = 77/88 (87.50%), Query Frame = 1 
Query:   40 SEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLNTEEGF 303
            SEAG++LKI V NFMCHRKL++ LCK VNFI+GRNGSGKSAILAALQICLGA+A LTHRA ++ D IRHG KGDA LEVTLLNTE GF
Sbjct:  174 SEAGVVLKINVSNFMCHRKLTVPLCKQVNFINGRNGSGKSAILAALQICLGAKAHLTHRAKKMTDFIRHGWKGDAVLEVTLLNTEHGF 261
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: A0A836CML4_9STRA (P-loop containing nucleoside triphosphate hydrolase protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CML4_9STRA)

HSP 1 Score: 133 bits (335), Expect = 4.080e-34
Identity = 63/89 (70.79%), Postives = 79/89 (88.76%), Query Frame = 1 
Query:   40 SEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLNTEEGFK 306
            SE+G+IL+I V+NFMCHR L++ LC+++NFI+G NGSGKSAILAALQICLGARAR+THRA +LEDLIRHG  GDA++EV +LNT +G K
Sbjct:   72 SESGVILRIDVKNFMCHRHLTVDLCRNINFINGSNGSGKSAILAALQICLGARARVTHRAHKLEDLIRHGWGGDAEVEVKMLNTPDGHK 160
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: W7U0X2_9STRA (Structural maintenance of chromosomes 6 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U0X2_9STRA)

HSP 1 Score: 121 bits (304), Expect = 6.200e-30
Identity = 58/83 (69.88%), Postives = 68/83 (81.93%), Query Frame = 1 
Query:   40 SEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLN 288
            +E G+I+KI  ENFMCHRKLS+ LC +VNFIHG+NGSGKSAILAALQICLGARA +THR  RL D+IR G  G A + V+LLN
Sbjct:  145 AETGVIVKIYCENFMCHRKLSINLCPNVNFIHGQNGSGKSAILAALQICLGARANVTHRGARLSDMIRQGHDGHAIVRVSLLN 227
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: A0A7S2KU12_9STRA (Hypothetical protein (Fragment) n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2KU12_9STRA)

HSP 1 Score: 119 bits (299), Expect = 2.150e-29
Identity = 62/92 (67.39%), Postives = 74/92 (80.43%), Query Frame = 1 
Query:   40 SEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGD---AKLEVTLLNT-EEGF 303
            +EAGII ++ VENFMCHRKLS+ LC++VNFIHG+NGSGKSAILAA+Q+CLGA AR THRA  L+DL+R  A  D   AKL VTLLN   +GF
Sbjct:  111 AEAGIIQEVYVENFMCHRKLSVKLCRNVNFIHGQNGSGKSAILAAIQVCLGAGARRTHRARNLKDLVRKEAGADCSGAKLRVTLLNKGSDGF 202
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: B8BRT7_THAPS (Smc-like protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BRT7_THAPS)

HSP 1 Score: 118 bits (296), Expect = 7.440e-29
Identity = 60/86 (69.77%), Postives = 71/86 (82.56%), Query Frame = 1 
Query:   40 SEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGD---AKLEVTLLN 288
            +EAGII ++ VENFMCHRKLS+ LC++VNFIHG+NGSGKSAILAA+Q+CLGA AR THRA  L+DL+R  A  D   AKL VTLLN
Sbjct:   97 AEAGIINEVYVENFMCHRKLSVKLCRNVNFIHGQNGSGKSAILAAIQVCLGAGARRTHRARNLKDLVRKEAGADCTGAKLRVTLLN 182
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: A0A7S2UR98_9STRA (Hypothetical protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2UR98_9STRA)

HSP 1 Score: 116 bits (291), Expect = 3.510e-28
Identity = 58/91 (63.74%), Postives = 75/91 (82.42%), Query Frame = 1 
Query:   40 SEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIR-HGAKGDAKLEVTLLNT-EEGFK 306
            +EAGII ++ V++FMCHRKL++ LC++VNFIHG NGSGKSAILAA+QICLGA AR THRA  L++L+R  G+   AK+ VTLLN  E+G+K
Sbjct:   59 AEAGIIKRVYVQDFMCHRKLTVDLCQNVNFIHGANGSGKSAILAAIQICLGAHARRTHRARNLKELVRKEGSNSGAKVRVTLLNGGEDGYK 149
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: F0YEV5_AURAN (SMC_N domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YEV5_AURAN)

HSP 1 Score: 106 bits (264), Expect = 1.210e-27
Identity = 50/84 (59.52%), Postives = 66/84 (78.57%), Query Frame = 1 
Query:   37 TSEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLN 288
            +S AGII +I  E+FMCH+KL++  C+ +NFI+G NGSGKSAILAALQICLGA A+ THR  R+ DL+R G +G A + V+L+N
Sbjct:    8 SSSAGIIHEIYCEHFMCHKKLTIKPCRRINFINGANGSGKSAILAALQICLGASAKSTHRGNRMGDLVREGYEGQALVRVSLVN 91
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: A0A8J9SZH1_PHATR (Hypothetical protein (Fragment) n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=A0A8J9SZH1_PHATR)

HSP 1 Score: 108 bits (269), Expect = 2.200e-27
Identity = 55/84 (65.48%), Postives = 65/84 (77.38%), Query Frame = 1 
Query:   43 EAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRH--GAKGDAKLEVTLLN 288
            EAGII ++ VENFMCH KL +TLC++VNFIHG+NGSGKSAILAA+QICLGA A  THRA  L+ L+R   G    AK+ VTL N
Sbjct:   62 EAGIIKEVYVENFMCHPKLRVTLCRNVNFIHGQNGSGKSAILAAIQICLGASASRTHRARNLKGLVRKDAGPNATAKVRVTLWN 145
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BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Match: A0A662YEN6_9STRA (AAA_23 domain-containing protein (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662YEN6_9STRA)

HSP 1 Score: 114 bits (285), Expect = 2.260e-27
Identity = 51/89 (57.30%), Postives = 69/89 (77.53%), Query Frame = 1 
Query:   34 VTSEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILAALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLNTEEG 300
            + SE G++ +I  ENFMCHRK+ + LC H+NFI G NGSGKSAI+AA+QICLGA AR+THR   +++LIRHG +G+A + +TL N  +G
Sbjct:  108 IDSEMGVVEEIYCENFMCHRKMRVALCPHINFITGENGSGKSAIIAAIQICLGASARVTHRGKSIKNLIRHGHEGNALVRITLRNDAKG 196
The following BLAST results are available for this feature:
BLAST of mRNA_D-mesarthrocarpus_Contig1082.6.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G4M1_ECTSI5.680e-3972.28Smc-like protein n=1 Tax=Ectocarpus siliculosus Ta... [more]
D7G4M2_ECTSI1.730e-3778.41Smc-like protein n=2 Tax=Ectocarpus TaxID=2879 Rep... [more]
A0A836CML4_9STRA4.080e-3470.79P-loop containing nucleoside triphosphate hydrolas... [more]
W7U0X2_9STRA6.200e-3069.88Structural maintenance of chromosomes 6 n=2 Tax=Mo... [more]
A0A7S2KU12_9STRA2.150e-2967.39Hypothetical protein (Fragment) n=1 Tax=Skeletonem... [more]
B8BRT7_THAPS7.440e-2969.77Smc-like protein n=1 Tax=Thalassiosira pseudonana ... [more]
A0A7S2UR98_9STRA3.510e-2863.74Hypothetical protein n=1 Tax=Attheya septentrional... [more]
F0YEV5_AURAN1.210e-2759.52SMC_N domain-containing protein (Fragment) n=1 Tax... [more]
A0A8J9SZH1_PHATR2.200e-2765.48Hypothetical protein (Fragment) n=2 Tax=Phaeodacty... [more]
A0A662YEN6_9STRA2.260e-2757.30AAA_23 domain-containing protein (Fragment) n=2 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-mesarthrocarpus_Contig1082contigD-mesarthrocarpus_Contig1082:21685..23744 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Discosporangium mesarthrocarpum MT17_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7G4M1
Preferred nameSMC6
PFAMsAAA_23,SMC_N
Model size309
Max annot lvl2759|Eukaryota
KEGG koko:K12614,ko:K15014
KEGG TC2.A.57.1
KEGG Pathwayko03018,map03018
KEGG ModuleM00395
Hectar predicted targeting categoryother localisation
GOsGO:0000228,GO:0000722,GO:0000723,GO:0000724,GO:0000725,GO:0000781,GO:0000793,GO:0000794,GO:0000803,GO:0001674,GO:0003674,GO:0003676,GO:0003677,GO:0003684,GO:0003697,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005737,GO:0005739,GO:0006139,GO:0006259,GO:0006281,GO:0006302,GO:0006310,GO:0006312,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007049,GO:0007059,GO:0007568,GO:0007569,GO:0008094,GO:0008150,GO:0008152,GO:0009410,GO:0009987,GO:0010033,GO:0010243,GO:0014070,GO:0014074,GO:0016043,GO:0016462,GO:0016604,GO:0016605,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019899,GO:0022402,GO:0030915,GO:0031000,GO:0031625,GO:0031974,GO:0031981,GO:0032200,GO:0032502,GO:0032991,GO:0033554,GO:0033676,GO:0034641,GO:0035061,GO:0035861,GO:0036270,GO:0042221,GO:0042493,GO:0042585,GO:0042592,GO:0042623,GO:0043073,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043279,GO:0044237,GO:0044238,GO:0044260,GO:0044389,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0048518,GO:0048522,GO:0048869,GO:0050000,GO:0050789,GO:0050794,GO:0050896,GO:0051179,GO:0051276,GO:0051304,GO:0051640,GO:0051641,GO:0051716,GO:0051983,GO:0051984,GO:0060249,GO:0065007,GO:0065008,GO:0070013,GO:0071139,GO:0071704,GO:0071840,GO:0090304,GO:0090398,GO:0090734,GO:0097159,GO:0097240,GO:0098687,GO:0106068,GO:1901360,GO:1901363,GO:1901698,GO:1902494,GO:1990234,GO:1990683
Exons3
Evalue6.14e-39
EggNOG OGsCOG1196@1|root,KOG0250@2759|Eukaryota
EC3.6.4.13
Descriptiondouble-strand break repair via homologous recombination
Cds size288
COG categoryD
BRITEko00000,ko00001,ko00002,ko01000,ko02000,ko03019,ko03036
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789215.688932-UTR-D-mesarthrocarpus_Contig1082:21684..217051680789215.688932-UTR-D-mesarthrocarpus_Contig1082:21684..21705Discosporangium mesarthrocarpum MT17_79UTRD-mesarthrocarpus_Contig1082 21685..21705 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789215.7119024-CDS-D-mesarthrocarpus_Contig1082:21705..218001680789215.7119024-CDS-D-mesarthrocarpus_Contig1082:21705..21800Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig1082 21706..21800 +
1680789215.7276618-CDS-D-mesarthrocarpus_Contig1082:22506..226321680789215.7276618-CDS-D-mesarthrocarpus_Contig1082:22506..22632Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig1082 22507..22632 +
1680789215.7367396-CDS-D-mesarthrocarpus_Contig1082:23677..237441680789215.7367396-CDS-D-mesarthrocarpus_Contig1082:23677..23744Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig1082 23678..23744 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-mesarthrocarpus_Contig1082.6.1prot_D-mesarthrocarpus_Contig1082.6.1Discosporangium mesarthrocarpum MT17_79polypeptideD-mesarthrocarpus_Contig1082 21706..23744 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-mesarthrocarpus_Contig1082.6.1

>prot_D-mesarthrocarpus_Contig1082.6.1 ID=prot_D-mesarthrocarpus_Contig1082.6.1|Name=mRNA_D-mesarthrocarpus_Contig1082.6.1|organism=Discosporangium mesarthrocarpum MT17_79|type=polypeptide|length=96bp
MRGMVTSEAGIILKIMVENFMCHRKLSLTLCKHVNFIHGRNGSGKSAILA
ALQICLGARARLTHRATRLEDLIRHGAKGDAKLEVTLLNTEEGFK*
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mRNA from alignment at D-mesarthrocarpus_Contig1082:21685..23744+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-mesarthrocarpus_Contig1082.6.1 ID=mRNA_D-mesarthrocarpus_Contig1082.6.1|Name=mRNA_D-mesarthrocarpus_Contig1082.6.1|organism=Discosporangium mesarthrocarpum MT17_79|type=mRNA|length=2060bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig1082:21685..23744+ (Discosporangium mesarthrocarpum MT17_79)
AGTAGGGGGGGTGTCAAGGGCATGAGGGGCATGGTAACTTCCGAGGCAGG
GATCATCCTCAAGATTATGGTCGAGAACTTCATGTGCCACCGGAAGCTCT
CACTCACCCTGTGCAAGTAAGTAGACAGGTTTACAAGGTTAGGGTTAGGG
TGAGGGTTAGGGTTGGGCTTGTGGGTTAAGAGTCAGGGTCAGGGTCAGGG
TTAGGGTGGGGTTAAGTTCATGGGGGAGGGTGAGAGTTAGGGTTGAGGTT
GAGGGTATAGGTTAGGGTTAAGAGTCAGGGGTGTAGGGAGAGTTAGGGTT
AGGGTTAGGGTTAGGGTTAGGATTAGGGTCATACCATGGTTGTAGCTCTA
ACCCTAGCAGGCGATGGGTGGTTTAGGGTTAGGGGTGGGGGTAGGGGTAG
GGGTAGGGTTAAAGCAAAGATTGACAAGGATCACAGGTTTAGAGTTAAGG
CTGGGGGTAAGGTTAGGGTCAGCAAGGTGAGATAGGTGCAGTGCTACTTC
TCTATCCCTAACCCTAACCCTAACCCTAACTCAAACCCTAACCTTAGCTT
GGCGAAGTGGAAGGCCAGGGAAGAAACATTAGGGTTAGGGTTCCAATCCC
TAACCTTGGCCATAAGAACATGTCAACAATCTTTCCGCTAAAATTGCTGA
CCCTACCCCTACCCCTAACCTATCACCAACCCTAAACCCTATCCTTATTG
CTAACCCTGTCCGTACCCCTAACCCTAACCCTAGTCCTAGTATCACCAAC
CCCAAGGACCCTGACCCTAACACCAACCCTCTCTCTTGCGGAAACAAACA
AACAAACAAATAAACAACAAAGGCATGTGAACTTCATCCATGGGCGCAAC
GGGTCAGGGAAGTCGGCCATCCTGGCAGCGCTTCAGATATGCCTTGGTGC
AAGGGCCAGGCTCACCCACAGAGCCACCAGGCTTGAAGACCTCATCAGGT
GCGCTACTGTACCGTGTACTGTATGTGCACTGTAACCTCAACCCTTTCCC
AACCCTAACACACAGAGCCACCAGGCTGGAAGACCTTATCAGGTGTATCC
TGTTCACGTTGTATCCCTAGCCCTATCCATAATATCTATCTTGCAAGGCA
TGGAATGTACATTATTACCCAAACCACAACCCCAAGAAAGTTCTCTTCAG
GTATGCATTTTCAGAGCTCCAGAGGCACTGTAGAGCTCCAGAGCTATATG
TAGAACTCAAGAACTGTATCTGAAGCTCTGGAGCCACATGAAGAGCTTCA
AAGTCACATCCAGGCGTTGTTTTGTGTGCTCAGTGTGTCTCTTGCATCTC
ATTAGTATTGTCTTCCTTCAGGGTCATAGTAGGGGTAACCTTGGGCTGGG
GAATGGTAGGGTTAGGGTTGGAGTCAGGGTAAGGCTCAGTCGCTTTCTTC
TCTTTTTTCTCTCCTTTATTCTGATGTAGGTGTGGGCTTTAAAGGGTTAG
GGTTGGTTCTAGAGCTCTATAGAACGGCAATATGGACCTTAGGGTTTGGG
TTTGGGTTATGCTTATGGTTATGGGTAGGGGTAGGGGTAGGGGTAGGGGT
ACGGTTTAGGTCAGGCTTACCCTCCACACCACCAGGCTGGAATAGTCAAA
CCCTAACCTGAATCCTAACTGTACCCTGAAAGCCTTCCTTTAGGAAAAAT
TTTGACGCTCTACTTCCAGCTATGGTACCAAGTGCTCTGAACGGTACATT
TTCAGTATGTTTTTCGGCATGTTTTTCAGTAATTTTTTTATCTCGGTGAT
ATCTCGGCATCCTTTCCGTTTCTGTTTGCTGGTAGCTAATAAGGACAACC
CTGAGCCTAACCCTATCCCTAAAACCAATGCTAGCCCTTAAGCTAAGCCT
GACAGTAGATCTGATCCCAACCCTAAACCTAAACCTGAAACTAGACCTGA
CCCTAACCCCAAACCTAACCCTGACACTTTGCCCAGACCTTATCAATGAC
TCTGATTCAAACCCTAATACTAAAGCTAACCCCGATCCTTCAGGCACGGT
GCCAAGGGGGATGCAAAGCTGGAAGTGACCCTTCTCAACACTGAGGAGGG
GTTCAAGTGA
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Coding sequence (CDS) from alignment at D-mesarthrocarpus_Contig1082:21685..23744+

>mRNA_D-mesarthrocarpus_Contig1082.6.1 ID=mRNA_D-mesarthrocarpus_Contig1082.6.1|Name=mRNA_D-mesarthrocarpus_Contig1082.6.1|organism=Discosporangium mesarthrocarpum MT17_79|type=CDS|length=288bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig1082:21685..23744+ (Discosporangium mesarthrocarpum MT17_79)
ATGAGGGGCATGGTAACTTCCGAGGCAGGGATCATCCTCAAGATTATGGT
CGAGAACTTCATGTGCCACCGGAAGCTCTCACTCACCCTGTGCAAGCATG
TGAACTTCATCCATGGGCGCAACGGGTCAGGGAAGTCGGCCATCCTGGCA
GCGCTTCAGATATGCCTTGGTGCAAGGGCCAGGCTCACCCACAGAGCCAC
CAGGCTTGAAGACCTCATCAGGCACGGTGCCAAGGGGGATGCAAAGCTGG
AAGTGACCCTTCTCAACACTGAGGAGGGGTTCAAGTGA
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