mRNA_D-dudresnayi_contig975.28542.1 (mRNA) Desmarestia dudresnayi DdudBR16 monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-dudresnayi_contig975.28542.1
Unique NamemRNA_D-dudresnayi_contig975.28542.1
TypemRNA
OrganismDesmarestia dudresnayi DdudBR16 monoicous (Desmarestia dudresnayi DdudBR16 monoicous)
Homology
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A6H5KKD9_9PHAE (ABC protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KKD9_9PHAE)

HSP 1 Score: 226 bits (575), Expect = 2.670e-65
Identity = 114/145 (78.62%), Postives = 125/145 (86.21%), Query Frame = 1
Query:    1 GLVCGLVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVT-AYTNPMTGDEVLRCFGFDGGRYFVDLAILAGVGVCGLVLAFVLLKRSR 432
            GL  GLVAFNLSLAG+LAACGAGAKSSQEALA+GCLVVLFSALLSGFLV+KD+LPA WG LAWLSP+GRGFE+LVANEFSPYGA+FRLSTKIG+    YT+PMTGD++LRCFGF  GR   DL ILA VG  GL LA V LKRSR
Sbjct: 1925 GLAGGLVAFNLSLAGVLAACGAGAKSSQEALATGCLVVLFSALLSGFLVSKDDLPAAWGALAWLSPIGRGFESLVANEFSPYGAVFRLSTKIGSAPIVYTDPMTGDQILRCFGFSSGRTLTDLGILAAVGGGGLALALVFLKRSR 2069          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A6H5L0D0_9PHAE (ABC protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0D0_9PHAE)

HSP 1 Score: 186 bits (472), Expect = 2.570e-55
Identity = 89/143 (62.24%), Postives = 111/143 (77.62%), Query Frame = 1
Query:    1 GLVCGLVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILAGVGVCGLVLAFVLLKRS 429
            GL  GLV FN+++A +LAACGAGA+S QEALA GCL VLFSALLSGFLVA+D+LP  WG L W SP+  GFEAL+ NEFS YGA+F L+T I  VTA   P+TGD +L CFGF+ GR+ +D+ +LA +G  GL+LA+ LLKR+
Sbjct:  191 GLCAGLVVFNVAVAAVLAACGAGARSPQEALAMGCLFVLFSALLSGFLVARDDLPGVWGGLLWASPIAHGFEALLINEFSEYGALFTLTTVISGVTASVGPLTGDSILSCFGFENGRFSMDMGLLAAIGGAGLLLAYALLKRA 333          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: D8LNV5_ECTSI (ATP-binding cassette superfamily n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNV5_ECTSI)

HSP 1 Score: 156 bits (394), Expect = 5.940e-41
Identity = 77/139 (55.40%), Postives = 99/139 (71.22%), Query Frame = 1
Query:   13 GLVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILAGVGVCGLVLAFVLLKRS 429
            GLV FN+++A +LAACGAGA+S QEALA GCL VLFSALLSGFLVA+D+LP  WG L W SP+  G           YGA+F L+T I  VTA   P++GD +L CFGF+ GR+ +D+ +L  +G  GL+LA+ LLKR+
Sbjct: 1854 GLVVFNVAVAAVLAACGAGARSPQEALAMGCLFVLFSALLSGFLVARDDLPGVWGGLLWASPIAHG----------EYGALFTLTTVISGVTASVGPLSGDNILSCFGFENGRFSLDMGLLVAIGGAGLLLAYALLKRA 1982          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A835W233_9CHLO (Uncharacterized protein n=1 Tax=Chlamydomonas schloesseri TaxID=2026947 RepID=A0A835W233_9CHLO)

HSP 1 Score: 71.6 bits (174), Expect = 2.040e-11
Identity = 47/135 (34.81%), Postives = 69/135 (51.11%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILA--GVGVCGLVLAFV 414
            LV+ N++ A +  A GA A S+  A   G L ++   L  GFL+ KD +PA    ++  S     +EAL  NEF  +   F  +  I T       ++GD VL+ FGFD   +++DL +LA  G   CGL   F+
Sbjct:  999 LVSTNITAATMCQAIGAAAPSNAVANLVGSLTLMLLLLFGGFLLNKDAVPAYCAWVSRASFFNYAYEALAINEFHHFPLDFTFTAPINTSALPPLRISGDGVLKEFGFDVDLFYLDLTMLAVLGAACCGLTYFFL 1133          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A835XJI5_9CHLO (Uncharacterized protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A835XJI5_9CHLO)

HSP 1 Score: 69.3 bits (168), Expect = 1.290e-10
Identity = 46/138 (33.33%), Postives = 68/138 (49.28%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILAGVGVCGLVLAFVLLKRS 429
            LV+ N++ A +  A GAG+ S+  A   G L ++   L  GFL+ KD +P+    L   S     +EAL  NEF  +   F  +  I T       ++GD VL+ FGFD  R++ D A L  +G       ++LL  S
Sbjct: 1041 LVSTNITAATLCMAVGAGSPSNAIANLVGSLGLMLLLLFGGFLLNKDAVPSYCSWLEAASFFNYAYEALAVNEFHHFPLDFTFTAPINTSVLPPLRISGDGVLKEFGFDVDRFYADYAALLALGAAFCGATYLLLYFS 1178          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: D8TRH3_VOLCA (Uncharacterized protein n=1 Tax=Volvox carteri f. nagariensis TaxID=3068 RepID=D8TRH3_VOLCA)

HSP 1 Score: 67.4 bits (163), Expect = 5.990e-10
Identity = 44/138 (31.88%), Postives = 70/138 (50.72%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILAGVGVCGLVLAFVLLKRS 429
            LV+ N++ A +  A GA A S+  A   G L ++   L  GFL+ KD +P+    ++  S     +EAL  NEF  +   F  +  I T       ++GD VL+ FGFD   +++D+ +L  +G     L ++LL  S
Sbjct:  997 LVSSNITAATMCMAIGAAAPSNSVANLVGSLTLMLLLLFGGFLLNKDSVPSYCAWISKASFFNYAYEALAINEFHRFPRDFTFTAPIKTSALPPLRISGDGVLKEFGFDVDLFYLDVIMLGLLGAVCCGLTYILLYFS 1134          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A250XNY5_9CHLO (Uncharacterized protein n=1 Tax=Chlamydomonas eustigma TaxID=1157962 RepID=A0A250XNY5_9CHLO)

HSP 1 Score: 67.0 bits (162), Expect = 8.100e-10
Identity = 46/139 (33.09%), Postives = 71/139 (51.08%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVD--LAILAGVGVCGLVLAFVLLKR 426
            LV  N++ A +  A GA A S+  A  +G L ++   L  GFL+ K+++P     ++ LS     +EAL  NEF  + A F  +  I +       +TGD VL+ FGF+   +  D  L ++  +  CGL  A+ LL R
Sbjct: 1041 LVGANVAAAAMCMAIGAAAPSNSVANMAGSLTLMLLLLFGGFLLNKEKVPVYSRWISSLSFFNYAYEALAVNEFHGFPADFSFTAPIDSSALPPLRITGDGVLKEFGFEQDAFLSDEVLLVILALTFCGL--AYYLLNR 1177          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A8J4AKR2_9CHLO (Uncharacterized protein n=1 Tax=Volvox africanus TaxID=51714 RepID=A0A8J4AKR2_9CHLO)

HSP 1 Score: 64.3 bits (155), Expect = 6.980e-9
Identity = 50/163 (30.67%), Postives = 77/163 (47.24%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILAGVGVCGLVLAFVLLKRSR**RRTWFSFWRGVFCVCGVLLKGG 504
            LV+ N++ A +  A GA A S+  A   G L ++   L  GFL+ K  +P     ++ +S     +EAL  NEF  +   F  +  I T       +TG+ VL+ FGF+   +++D+ +L  +G     L +VLL  S       F    G   V  VLL+ G
Sbjct: 1038 LVSSNITAATMCMAIGAAAPSNPIANLVGSLTLMLLLLFGGFLLNKGSVPPYCAWISKVSFFNYAYEALAINEFHYFPEDFTFTAPINTTKLPPLRVTGEGVLKEFGFNVDLFYLDVFMLGILGTLCCALTYVLLYFSGHTLLDDFEDLTGR-TVAWVLLRAG 1199          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A8J4D8V3_9CHLO (ABC transporter domain-containing protein n=1 Tax=Volvox reticuliferus TaxID=1737510 RepID=A0A8J4D8V3_9CHLO)

HSP 1 Score: 56.2 bits (134), Expect = 3.920e-6
Identity = 37/121 (30.58%), Postives = 60/121 (49.59%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGGRYFVDLAILA 378
            LV+ N++ A +  A GA A S+  A   G L ++   L  GFL+ K  +P     ++  S     +EAL  NEF  +   F  +  I T       +TG+ VL+ FGF+   +++D++  A
Sbjct:  638 LVSSNITAATMCMAIGAAAPSNPIANLVGSLTLMLLLLFGGFLLNKGSVPPYCAWVSKASFFNYAYEALAINEFHSFPGDFTFTPPINTTKLPPLRVTGEGVLKEFGFNVDLFYLDVSCWA 758          
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Match: A0A7R9YQJ6_9CHLO (Hypothetical protein n=1 Tax=Chlamydomonas euryale TaxID=1486919 RepID=A0A7R9YQJ6_9CHLO)

HSP 1 Score: 53.9 bits (128), Expect = 1.820e-5
Identity = 47/151 (31.13%), Postives = 70/151 (46.36%), Query Frame = 1
Query:   16 LVAFNLSLAGILAACGAGAKSSQEALASGCLVVLFSALLSGFLVAKDELPAGWGVLAWLSPMGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNP-----------MTGDEVLRCFGFD-GGRYFVDLAILAGVGVCGLVLAFVLLKRSR 432
            LV  N++   +  A GAG  S   A  +G L VL  ALL GFL++++ +PA     + LS +   FE L+ NEF      FR        TA+  P           ++GDE+L+ FGF       +D  +  G+ V   +   +LL  +R
Sbjct:  156 LVLHNMASTALSMALGAGLPSVAAANMAGSLAVLACALLGGFLLSRNRMPAVVDWASSLSYVRYSFEGLLINEFHGVDG-FRF-------TAFHQPGVPSDKVPHVDVSGDEILQTFGFRISDEALLDDTLRLGLLVAAFLGGTLLLLHAR 298          
The following BLAST results are available for this feature:
BLAST of mRNA_D-dudresnayi_contig975.28542.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
A0A6H5KKD9_9PHAE2.670e-6578.62ABC protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0... [more]
A0A6H5L0D0_9PHAE2.570e-5562.24ABC protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 Ta... [more]
D8LNV5_ECTSI5.940e-4155.40ATP-binding cassette superfamily n=1 Tax=Ectocarpu... [more]
A0A835W233_9CHLO2.040e-1134.81Uncharacterized protein n=1 Tax=Chlamydomonas schl... [more]
A0A835XJI5_9CHLO1.290e-1033.33Uncharacterized protein n=1 Tax=Edaphochlamys deba... [more]
D8TRH3_VOLCA5.990e-1031.88Uncharacterized protein n=1 Tax=Volvox carteri f. ... [more]
A0A250XNY5_9CHLO8.100e-1033.09Uncharacterized protein n=1 Tax=Chlamydomonas eust... [more]
A0A8J4AKR2_9CHLO6.980e-930.67Uncharacterized protein n=1 Tax=Volvox africanus T... [more]
A0A8J4D8V3_9CHLO3.920e-630.58ABC transporter domain-containing protein n=1 Tax=... [more]
A0A7R9YQJ6_9CHLO1.820e-531.13Hypothetical protein n=1 Tax=Chlamydomonas euryale... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-dudresnayi_contig975contigD-dudresnayi_contig975:28379..29568 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Desmarestia dudresnayi DdudBR16 monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score115.2
Seed ortholog evalue1.4e-23
Seed eggNOG ortholog2880.D8LNU0
KEGG koko:K05681
KEGG TC3.A.1.204
KEGG Pathwayko01523,ko02010,ko04976,map01523,map02010,map04976
EggNOG free text desc.ATPase activity, coupled to transmembrane movement of substances
EggNOG OGsCOG1131@1,KOG0061@2759
COG Functional cat.V
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02000,ko04090,ko04147
Hectar predicted targeting categorysignal anchor
Exons2
Model size504
Cds size237
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-dudresnayi_contig975.28542.1prot_D-dudresnayi_contig975.28542.1Desmarestia dudresnayi DdudBR16 monoicouspolypeptideD-dudresnayi_contig975 28448..29370 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930930.4566298-UTR-D-dudresnayi_contig975:28378..284471622930930.4566298-UTR-D-dudresnayi_contig975:28378..28447Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig975 28379..28447 -
1690557969.581612-UTR-D-dudresnayi_contig975:28378..284471690557969.581612-UTR-D-dudresnayi_contig975:28378..28447Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig975 28379..28447 -
1622930930.5211763-UTR-D-dudresnayi_contig975:29370..295681622930930.5211763-UTR-D-dudresnayi_contig975:29370..29568Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig975 29371..29568 -
1690557969.6195157-UTR-D-dudresnayi_contig975:29370..295681690557969.6195157-UTR-D-dudresnayi_contig975:29370..29568Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig975 29371..29568 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930930.4739468-CDS-D-dudresnayi_contig975:28447..286431622930930.4739468-CDS-D-dudresnayi_contig975:28447..28643Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig975 28448..28643 -
1690557969.6030235-CDS-D-dudresnayi_contig975:28447..286431690557969.6030235-CDS-D-dudresnayi_contig975:28447..28643Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig975 28448..28643 -
1622930930.4939651-CDS-D-dudresnayi_contig975:29329..293701622930930.4939651-CDS-D-dudresnayi_contig975:29329..29370Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig975 29330..29370 -
1690557969.611304-CDS-D-dudresnayi_contig975:29329..293701690557969.611304-CDS-D-dudresnayi_contig975:29329..29370Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig975 29330..29370 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-dudresnayi_contig975.28542.1

>prot_D-dudresnayi_contig975.28542.1 ID=prot_D-dudresnayi_contig975.28542.1|Name=mRNA_D-dudresnayi_contig975.28542.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=polypeptide|length=79bp
MGRGFEALVANEFSPYGAIFRLSTKIGTVTAYTNPMTGDEVLRCFGFDGG
RYFVDLAILAGVGVCGLVLAFVLLKRSR*
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mRNA from alignment at D-dudresnayi_contig975:28379..29568-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-dudresnayi_contig975.28542.1 ID=mRNA_D-dudresnayi_contig975.28542.1|Name=mRNA_D-dudresnayi_contig975.28542.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=mRNA|length=1190bp|location=Sequence derived from alignment at D-dudresnayi_contig975:28379..29568- (Desmarestia dudresnayi DdudBR16 monoicous)
GGACTGGTCTGCGGATTGGTCGCATTTAACCTGTCGCTCGCCGGGATCCT AGCCGCGTGCGGTGCGGGTGCGAAGTCGTCACAGGAGGCCCTCGCTTCGG GATGTCTTGTGGTGCTGTTCTCGGCGTTGCTGTCGGGGTTCTTGGTGGCG AAGGACGAGTTGCCCGCCGGCTGGGGTGTGTTGGCGTGGTTGTCGCCGAT GGGGAGGGGGTTCGAGGCGCTCGTTGCGAACGAGTTTAGGTGAGGCGTTT CTGCGTTCGGGGAGGGGGGATACTGTACTATGACCCCTACCCCCCTGGGC ACATGCCTTGGTTTTTATCGAAAACAGGGTTCGTCGCTCCCTTACTGTAC TGTATATTTGTTGTGTTTGTGTGTGTGGGTGTTTTATGTGTTTGGTGCGA TTTTGTCATGGTGCATCCTGTCTTTGCAACGGGTGAAGGAATATCATTGA AGAAAAATAGACGATACGCAGTGGACTCTGCCGTTGGAGGCTGTTTTTTG TTGGAAATGCTTGTCTTGCCTGTAGATATGATGTGTTCGTTCTATTTTTT TCATGTTCATTTTTTTTATTTGTTCACGTTTGCCGAAAACAAAACGTTCC TCTCATCGTTCCTCGTGGCCGCCGGGAGAGGTATGGCTCGTAATCGCTGC CGGACAGATAAAACGTACCAGTACGTGACCTCCTGCGACAATTGCGTCAC GCGTACCGAGTATCCTCAAGAAACACGCAGGACATCGTTTGGCTCGTAGA TGAACACGTAACGGTAGAGCTTATAACTCGCGAGTAATGCCCCCCCCCCC TCTCACTCTTGACTGTTGAGTCAAGCCCACCGTTCCCCGTGTCCAACTTT ACCGTACCTCCCCCCTCCCCACTGTCTCTGGACCCTGCCCTTCCTCATCG TTTATCCGCGTTTTTATTTTATCAGTCCATACGGGGCCATCTTTCGGCTG TCGACCAAGATCGGCACTGTTACCGCCTACACGAACCCCATGACGGGAGA CGAGGTGCTGCGGTGCTTCGGCTTCGACGGGGGGCGTTACTTTGTCGATC TCGCGATTCTGGCTGGTGTTGGGGTTTGCGGTTTGGTGCTGGCGTTCGTT CTCTTGAAACGCTCGCGATGATGACGACGAACTTGGTTTTCCTTTTGGCG TGGCGTTTTTTGTGTGTGCGGGGTTTTGTTGAAAGGTGGG
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Coding sequence (CDS) from alignment at D-dudresnayi_contig975:28379..29568-

>mRNA_D-dudresnayi_contig975.28542.1 ID=mRNA_D-dudresnayi_contig975.28542.1|Name=mRNA_D-dudresnayi_contig975.28542.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=CDS|length=474bp|location=Sequence derived from alignment at D-dudresnayi_contig975:28379..29568- (Desmarestia dudresnayi DdudBR16 monoicous)
ATGGGGAGGGGGTTCGAGGCGCTCGTTGCGAACGAGTTTAGATGGGGAGG
GGGTTCGAGGCGCTCGTTGCGAACGAGTTTAGTCCATACGGGGCCATCTT
TCGGCTGTCGACCAAGATCGGCACTGTTACCGCCTACACGAACCCCATGA
CGGGAGACGAGGTGCTGCGGTGCTTCGGCTTCGACGGGGGGCGTTACTTT
GTCGATCTCGCGATTCTGGCTGGTGTTGGGGTTTGCGGTTTGGTGCTGGC
GTTCGTTCTCTTGAAACGCTCGCGATGATCCATACGGGGCCATCTTTCGG
CTGTCGACCAAGATCGGCACTGTTACCGCCTACACGAACCCCATGACGGG
AGACGAGGTGCTGCGGTGCTTCGGCTTCGACGGGGGGCGTTACTTTGTCG
ATCTCGCGATTCTGGCTGGTGTTGGGGTTTGCGGTTTGGTGCTGGCGTTC
GTTCTCTTGAAACGCTCGCGATGA
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