mRNA_D-dudresnayi_contig10572.644.1 (mRNA) Desmarestia dudresnayi DdudBR16 monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-dudresnayi_contig10572.644.1
Unique NamemRNA_D-dudresnayi_contig10572.644.1
TypemRNA
OrganismDesmarestia dudresnayi DdudBR16 monoicous (Desmarestia dudresnayi DdudBR16 monoicous)
Homology
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A7S3HF42_9STRA (Methylated-DNA--protein-cysteine methyltransferase n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HF42_9STRA)

HSP 1 Score: 136 bits (343), Expect = 3.580e-36
Identity = 73/138 (52.90%), Postives = 94/138 (68.12%), Query Frame = 2
Query:   86 NKITDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAMETKKTAEKESGNKRKRKNNEDETAVSVE-DTARVRISLSCVYRF 496
            +++T FD+KV+EACSRVPKGKVTTYK+IA+E+GS  A RAVGSSLRRNP+AP VPCHRVVS+   L GF+GSTDPR  +L KK  ML+ EGV  + K                 D T    E DT++ R++ +C++ F
Sbjct:   50 SRVTAFDRKVYEACSRVPKGKVTTYKEIAKEVGSPHAHRAVGSSLRRNPYAPVVPCHRVVSASGVLAGFHGSTDPRGEMLLKKRRMLIEEGVKFDFKGGI--------------DLTKPQDELDTSKARVNAACMFEF 173          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A7X7LEN7_9BACT (MGMT family protein n=1 Tax=bacterium TaxID=1869227 RepID=A0A7X7LEN7_9BACT)

HSP 1 Score: 104 bits (259), Expect = 1.430e-24
Identity = 55/99 (55.56%), Postives = 71/99 (71.72%), Query Frame = 2
Query:   92 ITDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAMETKKTAE 388
            +T F K+V++A  RVPKG VTTY  IAR IG   A RAVG +LRRNPFAP+VPCHRV++SD+++GGF G T    A +++K+ ML  EGV  +  K AE
Sbjct:    4 VTPFQKRVYDATRRVPKGMVTTYAMIARRIGCGSA-RAVGQALRRNPFAPEVPCHRVIASDMTIGGFSGRTG--GAQIRRKLRMLAAEGVVFKGGKLAE 99          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A3G4ZUM3_9VIRU (DNA_binding_1 domain-containing protein n=1 Tax=Edafosvirus sp. TaxID=2487765 RepID=A0A3G4ZUM3_9VIRU)

HSP 1 Score: 103 bits (257), Expect = 4.590e-24
Identity = 47/99 (47.47%), Postives = 70/99 (70.71%), Query Frame = 2
Query:   74 LNQNNKITDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAME 370
            ++  NKI+ F +KV++   ++PKGK+TTYK IA  IG   A  AVG++L  NPFAP++PCHRV+SS+ S+GG++G+ D  S  +Q K+ +L  EG+  E
Sbjct:    1 MSNKNKISPFAQKVYDVTKQIPKGKITTYKLIAIAIGKPGASVAVGTALANNPFAPKIPCHRVISSNYSIGGYFGTKDISSTNIQNKIKLLKKEGIEFE 99          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A3G5A9Y8_9VIRU (Cysteine methyltransferase n=1 Tax=Hyperionvirus sp. TaxID=2487770 RepID=A0A3G5A9Y8_9VIRU)

HSP 1 Score: 102 bits (255), Expect = 6.620e-24
Identity = 47/93 (50.54%), Postives = 69/93 (74.19%), Query Frame = 2
Query:   92 ITDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAME 370
            +T F + V++   ++PKGKV+TYK+IA+ +G   A +AVG +LRRNP+AP+VPCHRV+S+D SLGGF GST   S  L+KK+ +L  E + ++
Sbjct:    3 VTPFAQSVYDLTKQIPKGKVSTYKNIAKALGKPGACQAVGQALRRNPYAPEVPCHRVISTDFSLGGFIGSTAIDSDNLRKKIRLLKDENILID 95          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A0D2KYZ4_9CHLO (Methylated-DNA--protein-cysteine methyltransferase n=1 Tax=Monoraphidium neglectum TaxID=145388 RepID=A0A0D2KYZ4_9CHLO)

HSP 1 Score: 102 bits (254), Expect = 1.170e-23
Identity = 48/92 (52.17%), Postives = 67/92 (72.83%), Query Frame = 2
Query:   95 TDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAME 370
            T ++++V+  CS +PKG+VTTY D+A+ +      RAVG ++RRNPFAP+VPCHRVV+SDL LGGF GS    +A ++ K  ML+ EGV +E
Sbjct:    8 TAYEERVYTMCSAIPKGRVTTYGDMAKALSPPSCARAVGQAMRRNPFAPKVPCHRVVASDLKLGGFKGSWGTETANVKLKRQMLLEEGVEIE 99          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A2D6SPG3_9ARCH (Cysteine methyltransferase n=1 Tax=Candidatus Woesearchaeota archaeon TaxID=2026803 RepID=A0A2D6SPG3_9ARCH)

HSP 1 Score: 101 bits (252), Expect = 1.420e-23
Identity = 49/101 (48.51%), Postives = 72/101 (71.29%), Query Frame = 2
Query:  101 FDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAMETKKTAEKESGN 403
            F++K++    +VPKGKVTTYK +A++IG+ +A+RAVG ++R NP+AP+VPCHR VSSD  +GGF G   P S  ++KK+ ML  EG+ ++  K  + E  N
Sbjct:    5 FNEKIYSELKKVPKGKVTTYKALAKKIGT-KAYRAVGRAMRNNPYAPKVPCHRCVSSDGRIGGFSGKLSPNSREVKKKIRMLRNEGIKIKGDKVVDFERVN 104          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A851GMK0_9BACT (MGMT family protein n=1 Tax=Oceaniferula marina TaxID=2748318 RepID=A0A851GMK0_9BACT)

HSP 1 Score: 101 bits (251), Expect = 2.430e-23
Identity = 46/92 (50.00%), Postives = 71/92 (77.17%), Query Frame = 2
Query:   86 NKITDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGV 361
            +++TDF ++V+ A  R+P GKVTTY+++ARE+G   A +A+G +L+RNP+AP VPCHRV+ +DLS+GG+ G T+   A ++KK+ +L  EGV
Sbjct:    6 DRVTDFQRRVYAALERIPLGKVTTYRELARELGCGSA-QAIGQALKRNPYAPDVPCHRVIRTDLSIGGYSGQTE--GAKIEKKIRLLGEEGV 94          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: C5L0T6_PERM5 (Methylated-DNA--protein-cysteine methyltransferase n=1 Tax=Perkinsus marinus (strain ATCC 50983 / TXsc) TaxID=423536 RepID=C5L0T6_PERM5)

HSP 1 Score: 101 bits (251), Expect = 2.650e-23
Identity = 55/135 (40.74%), Postives = 79/135 (58.52%), Query Frame = 2
Query:   92 ITDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAMETKKTAEKESGNKRKRKNNEDETAVSVEDTARVRISLSCVYRF 496
            ++ F ++V+EA   VPKGKVTTYK+++R I   R+ +AVG +L+ NPFAP VPCHRVV + L +GGF+G     S  +Q+K+++L+ EGV                          V V+D  RVR+S  C+Y F
Sbjct:    8 VSPFRRRVYEALLEVPKGKVTTYKELSRRIDC-RSCQAVGQALKCNPFAPDVPCHRVVRASLDIGGFHGHVATDSKDVQRKIALLLEEGV--------------------------VFVDDNTRVRVSSECLYHF 115          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A7C3ZGW5_9ARCH (MGMT family protein n=1 Tax=Candidatus Woesearchaeota archaeon TaxID=2026803 RepID=A0A7C3ZGW5_9ARCH)

HSP 1 Score: 100 bits (249), Expect = 5.070e-23
Identity = 47/95 (49.47%), Postives = 72/95 (75.79%), Query Frame = 2
Query:   95 TDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGVAMETKK 379
            T+F +KV+E C +VPKG+VTTYK +A  +G  ++ +AVG +L+ NP+AP+VPCHRVV+SD ++GGF+G T+ +   +Q+K+ +L  EGV +E  K
Sbjct:   12 TEFQRKVYEKCCQVPKGRVTTYKRLAEALGV-KSSQAVGQALKVNPYAPRVPCHRVVASDGTIGGFFGQTEGKE--IQRKIKLLASEGVEVEGNK 103          
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Match: A0A2V0P5V8_9CHLO (Methylated-DNA--protein-cysteine methyltransferase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0P5V8_9CHLO)

HSP 1 Score: 101 bits (252), Expect = 6.720e-23
Identity = 43/89 (48.31%), Postives = 69/89 (77.53%), Query Frame = 2
Query:   95 TDFDKKVHEACSRVPKGKVTTYKDIAREIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSALLQKKVSMLVGEGV 361
            T ++++V+  CS +PKG+VTTY ++A+ +    + RAVG ++RRNPFAP+VPCHRVV++++ +GGF GS  P +A +++K +ML+ EGV
Sbjct:   35 TAYEQRVYRLCSAIPKGRVTTYGELAKALDPPSSARAVGQAMRRNPFAPRVPCHRVVAANMEIGGFTGSWGPEAASVKRKRAMLIEEGV 123          
The following BLAST results are available for this feature:
BLAST of mRNA_D-dudresnayi_contig10572.644.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3HF42_9STRA3.580e-3652.90Methylated-DNA--protein-cysteine methyltransferase... [more]
A0A7X7LEN7_9BACT1.430e-2455.56MGMT family protein n=1 Tax=bacterium TaxID=186922... [more]
A0A3G4ZUM3_9VIRU4.590e-2447.47DNA_binding_1 domain-containing protein n=1 Tax=Ed... [more]
A0A3G5A9Y8_9VIRU6.620e-2450.54Cysteine methyltransferase n=1 Tax=Hyperionvirus s... [more]
A0A0D2KYZ4_9CHLO1.170e-2352.17Methylated-DNA--protein-cysteine methyltransferase... [more]
A0A2D6SPG3_9ARCH1.420e-2348.51Cysteine methyltransferase n=1 Tax=Candidatus Woes... [more]
A0A851GMK0_9BACT2.430e-2350.00MGMT family protein n=1 Tax=Oceaniferula marina Ta... [more]
C5L0T6_PERM52.650e-2340.74Methylated-DNA--protein-cysteine methyltransferase... [more]
A0A7C3ZGW5_9ARCH5.070e-2349.47MGMT family protein n=1 Tax=Candidatus Woesearchae... [more]
A0A2V0P5V8_9CHLO6.720e-2348.31Methylated-DNA--protein-cysteine methyltransferase... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-dudresnayi_contig10572contigD-dudresnayi_contig10572:1048..2119 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Desmarestia dudresnayi DdudBR16 monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score99.4
Seed ortholog evalue1.6e-18
Seed eggNOG ortholog28583.AMAG_05236T0
KEGG koko:K00567
EggNOG free text desc.6-O-methylguanine DNA methyltransferase, DNA binding domain
EggNOG OGs3A8B1@33154,3Q3RJ@4751,COG0350@1,KOG4062@2759
EC2.1.1.63
COG Functional cat.L
Best tax levelFungi
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03400
Hectar predicted targeting categoryother localisation
Exons4
Model size856
Cds size480
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-dudresnayi_contig10572.644.1prot_D-dudresnayi_contig10572.644.1Desmarestia dudresnayi DdudBR16 monoicouspolypeptideD-dudresnayi_contig10572 1402..2097 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927561.5581455-UTR-D-dudresnayi_contig10572:1047..14011622927561.5581455-UTR-D-dudresnayi_contig10572:1047..1401Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig10572 1048..1401 -
1690556808.0341964-UTR-D-dudresnayi_contig10572:1047..14011690556808.0341964-UTR-D-dudresnayi_contig10572:1047..1401Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig10572 1048..1401 -
1622927561.6324139-UTR-D-dudresnayi_contig10572:2097..21191622927561.6324139-UTR-D-dudresnayi_contig10572:2097..2119Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig10572 2098..2119 -
1690556808.0925982-UTR-D-dudresnayi_contig10572:2097..21191690556808.0925982-UTR-D-dudresnayi_contig10572:2097..2119Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig10572 2098..2119 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927561.5806248-CDS-D-dudresnayi_contig10572:1401..16191622927561.5806248-CDS-D-dudresnayi_contig10572:1401..1619Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1402..1619 -
1690556808.045121-CDS-D-dudresnayi_contig10572:1401..16191690556808.045121-CDS-D-dudresnayi_contig10572:1401..1619Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1402..1619 -
1622927561.5922785-CDS-D-dudresnayi_contig10572:1711..17501622927561.5922785-CDS-D-dudresnayi_contig10572:1711..1750Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1712..1750 -
1690556808.0625975-CDS-D-dudresnayi_contig10572:1711..17501690556808.0625975-CDS-D-dudresnayi_contig10572:1711..1750Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1712..1750 -
1622927561.605896-CDS-D-dudresnayi_contig10572:1816..19181622927561.605896-CDS-D-dudresnayi_contig10572:1816..1918Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1817..1918 -
1690556808.0754223-CDS-D-dudresnayi_contig10572:1816..19181690556808.0754223-CDS-D-dudresnayi_contig10572:1816..1918Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1817..1918 -
1622927561.6210296-CDS-D-dudresnayi_contig10572:1976..20971622927561.6210296-CDS-D-dudresnayi_contig10572:1976..2097Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1977..2097 -
1690556808.084136-CDS-D-dudresnayi_contig10572:1976..20971690556808.084136-CDS-D-dudresnayi_contig10572:1976..2097Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10572 1977..2097 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-dudresnayi_contig10572.644.1

>prot_D-dudresnayi_contig10572.644.1 ID=prot_D-dudresnayi_contig10572.644.1|Name=mRNA_D-dudresnayi_contig10572.644.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=polypeptide|length=160bp
MTALKMSPVDDITSDLLLNQNNKITDFDKKVHEACSRVPKGKVTTYKDIA
REIGSERAFRAVGSSLRRNPFAPQVPCHRVVSSDLSLGGFYGSTDPRSAL
LQKKVSMLVGEGVAMETKKTAEKESGNKRKRKNNEDETAVSVEDTARVRI
SLSCVYRFD*
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mRNA from alignment at D-dudresnayi_contig10572:1048..2119-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-dudresnayi_contig10572.644.1 ID=mRNA_D-dudresnayi_contig10572.644.1|Name=mRNA_D-dudresnayi_contig10572.644.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=mRNA|length=1072bp|location=Sequence derived from alignment at D-dudresnayi_contig10572:1048..2119- (Desmarestia dudresnayi DdudBR16 monoicous)
TCCTACTACTGCGTTTCGATATATGACGGCATTGAAAATGAGTCCTGTAG ATGACATTACTTCAGATCTCCTACTGAATCAGAACAACAAAATTACTGAC TTTGATAAGAAAGTACACGAAGCTTGTTCCAGAGTTCCAAAAGGTAATAT AAAAGTATTAAATTTTAATATTTAGATAACAAGGAATATATTTTTTCACA GGAAAAGTGACGACATACAAAGATATTGCTCGTGAAATTGGAAGTGAAAG AGCTTTCAGAGCTGTTGGATCTTCTTTACGACGCAACCCTTTTGCTCCAC AAGAAAGATTAAATTCGAACAGTTTGCATGATATATTTTTTCTCAAATTC TTTTTGTGTTTGTTCTAGGTTCCGTGTCATCGGGTTGTCTCCTCGGATCT TTCACTGGGTATGTGAAGTTACCATACTACCGTACCGTACAGCACTACAA TATTATGTTTTATGCCTTCAATAATATTCCCTGTATAAATACTTTTATAG GAGGATTCTATGGAAGTACTGATCCTCGCAGTGCTCTGCTTCAGAAGAAG GTGTCGATGCTCGTCGGCGAAGGAGTTGCTATGGAGACCAAGAAAACTGC GGAAAAAGAGTCGGGAAATAAACGGAAAAGAAAGAATAATGAAGATGAAA CTGCTGTCTCTGTTGAAGATACTGCTCGTGTTCGTATTTCTTTGAGTTGT GTATATAGATTTGATTAAGATGTATGCAGATTGTATTAGCAGTATATGTG TTCATACAGTAGTACCGGTACCGGTACGATACAGTAGTGTGTACTGTACT GTATATGTCTGCAGCTGCAGATTGATCTTGTAACTCGACTTAGACTACAG TACGGTACCTTGTCTGCTCGTGTCAACCGTACAGTATCAGCAGGTGGTTC CCAGAATCCCTGCACTAACTTGTTCCCATGATCCCACTTGTAATGAGGAG AAGCACAATACTATTACAATCAATTTCGGATTACTGTATAGACAAATCAT AAAGCATCTACCATATTTTATTCATTCCTTGACTATATATATACTGCTGC TGGTATAGTTGCTATATAGTTG
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Coding sequence (CDS) from alignment at D-dudresnayi_contig10572:1048..2119-

>mRNA_D-dudresnayi_contig10572.644.1 ID=mRNA_D-dudresnayi_contig10572.644.1|Name=mRNA_D-dudresnayi_contig10572.644.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=CDS|length=960bp|location=Sequence derived from alignment at D-dudresnayi_contig10572:1048..2119- (Desmarestia dudresnayi DdudBR16 monoicous)
ATGACGGCATTGAAAATGAGTCCTGTAGATGACATTACTTCAGATCTCCT
ACTGAATCAGAACAACAAAATTACTGACTTTGATAAGAAAGTACACGAAG
CTTGTTCCAGAGTTCCAAAAGATGACGGCATTGAAAATGAGTCCTGTAGA
TGACATTACTTCAGATCTCCTACTGAATCAGAACAACAAAATTACTGACT
TTGATAAGAAAGTACACGAAGCTTGTTCCAGAGTTCCAAAAGGAAAAGTG
ACGACATACAAAGATATTGCTCGTGAAATTGGAAGTGAAAGAGCTTTCAG
AGCTGTTGGATCTTCTTTACGACGCAACCCTTTTGCTCCACAAGGAAAAG
TGACGACATACAAAGATATTGCTCGTGAAATTGGAAGTGAAAGAGCTTTC
AGAGCTGTTGGATCTTCTTTACGACGCAACCCTTTTGCTCCACAAGTTCC
GTGTCATCGGGTTGTCTCCTCGGATCTTTCACTGGTTCCGTGTCATCGGG
TTGTCTCCTCGGATCTTTCACTGGGAGGATTCTATGGAAGTACTGATCCT
CGCAGTGCTCTGCTTCAGAAGAAGGTGTCGATGCTCGTCGGCGAAGGAGT
TGCTATGGAGACCAAGAAAACTGCGGAAAAAGAGTCGGGAAATAAACGGA
AAAGAAAGAATAATGAAGATGAAACTGCTGTCTCTGTTGAAGATACTGCT
CGTGTTCGTATTTCTTTGAGTTGTGTATATAGATTTGATTAAGAGGATTC
TATGGAAGTACTGATCCTCGCAGTGCTCTGCTTCAGAAGAAGGTGTCGAT
GCTCGTCGGCGAAGGAGTTGCTATGGAGACCAAGAAAACTGCGGAAAAAG
AGTCGGGAAATAAACGGAAAAGAAAGAATAATGAAGATGAAACTGCTGTC
TCTGTTGAAGATACTGCTCGTGTTCGTATTTCTTTGAGTTGTGTATATAG
ATTTGATTAA
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