mRNA_D-dudresnayi_contig10495.543.1 (mRNA) Desmarestia dudresnayi DdudBR16 monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-dudresnayi_contig10495.543.1
Unique NamemRNA_D-dudresnayi_contig10495.543.1
TypemRNA
OrganismDesmarestia dudresnayi DdudBR16 monoicous (Desmarestia dudresnayi DdudBR16 monoicous)
Homology
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: D7G0B4_ECTSI (RGS domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G0B4_ECTSI)

HSP 1 Score: 160 bits (405), Expect = 6.130e-45
Identity = 75/116 (64.66%), Postives = 96/116 (82.76%), Query Frame = 1
Query:    1 QVVQLLFLLITGTVVLPLQDVWLDSHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFYKV 348
            QV QL FLL+TG+VV PLQ + LD+ H IRRIWV+YHN + SL+LFWPPI EPMYR+   D +YL SYT+GFSTLPTPAQ++ S +DQLSL++LR EF+KFA+ ++ARELP+FY+ 
Sbjct:  145 QVFQLAFLLLTGSVVFPLQHLLLDNKHGIRRIWVLYHNFLDSLVLFWPPIAEPMYRYLTGDVDYLNSYTQGFSTLPTPAQMKGSLRDQLSLDELRTEFQKFAENRMARELPEFYQA 260          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: D7G7J3_ECTSI (RGS domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7J3_ECTSI)

HSP 1 Score: 160 bits (404), Expect = 2.910e-43
Identity = 78/116 (67.24%), Postives = 94/116 (81.03%), Query Frame = 1
Query:    1 QVVQLLFLLITGTVVLPLQDVWLDSHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFYKV 348
            QV+QLLFL +TGT++ PL +  L  H  IRR W+MY N+++S+ILFWPPI EP+YR    D++YL SYTKGFS LPTPAQ+RS  KDQLSLE+LR EFEKFAD+KVARELPDFYK 
Sbjct:  175 QVLQLLFLFVTGTIIFPLLEESL--HSNIRRTWIMYDNIVSSIILFWPPIIEPLYRKLSGDEDYLYSYTKGFSQLPTPAQMRSCLKDQLSLEELRCEFEKFADSKVARELPDFYKA 288          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: A0A6H5K1F7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1F7_9PHAE)

HSP 1 Score: 144 bits (362), Expect = 1.540e-37
Identity = 81/173 (46.82%), Postives = 96/173 (55.49%), Query Frame = 1
Query:    1 QVVQLLFLLITGTVVLPLQDVWLDSHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQ---------------------------------------------------------LRSSFKDQLSLEQLRREFEKFADAKVARELPDFYKV 348
            QV QL FLL+TGTVV PLQ   LD  H+IRRIWV+YHN+  SL+LFWPPI EPMYR+   D+EYL SYT GFSTLPTPAQ                                                         ++SSF+DQLSLE+LR EF KFA+ ++ARELPDFY+ 
Sbjct:  508 QVFQLTFLLVTGTVVFPLQHAILDHRHEIRRIWVLYHNLFDSLVLFWPPIAEPMYRYLTGDEEYLHSYTHGFSTLPTPAQVQDSIPEHVVLDTELRQNILQPVTGCIAFVVFPSLHSCAVTAAIAHFPSSCFPFVSQMKSSFRDQLSLEELRTEFGKFAEERLARELPDFYQA 680          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: D8LI44_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LI44_ECTSI)

HSP 1 Score: 89.0 bits (219), Expect = 8.320e-20
Identity = 54/117 (46.15%), Postives = 65/117 (55.56%), Query Frame = 1
Query:    1 QVVQLLFLLITGTVVLPLQDVWLDSHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFYKVG 351
            QV QL FLL+TGTVV PLQ   LD  H+IRRIWV+YHN                  FF                      ++SSF+DQLSLE LR EFEKFA+ ++A+ELPDFY+ G
Sbjct:   79 QVFQLTFLLVTGTVVFPLQHAILDHRHEIRRIWVLYHN------------------FF----------------------MKSSFRDQLSLEDLRTEFEKFAEQRLAKELPDFYQGG 155          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: A0A6H5KDM3_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDM3_9PHAE)

HSP 1 Score: 92.4 bits (228), Expect = 1.620e-19
Identity = 48/90 (53.33%), Postives = 62/90 (68.89%), Query Frame = 1
Query:   73 SHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFY 342
             H  +RR+ ++Y  VI + IL W  I EP  +    DDEYL SYTKGFS LP+PAQL++S  +QLS+EQLR EF ++   KVA+EL DFY
Sbjct:   26 QHVTVRRVAILY-PVITTYILLWGSIREPFMKKILGDDEYLWSYTKGFSELPSPAQLKASLAEQLSVEQLRDEFRQYIKTKVAQELVDFY 114          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: D7FIS3_ECTSI (Similar to retinally abundant regulator of G-protein signaling hRGS-r n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIS3_ECTSI)

HSP 1 Score: 92.0 bits (227), Expect = 2.040e-19
Identity = 48/90 (53.33%), Postives = 62/90 (68.89%), Query Frame = 1
Query:   73 SHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFY 342
             H  +RR+ ++Y  VI + IL W  I EP  +    DDEYL SYTKGFS LP+PAQL++S  +QLS+EQLR EF ++   KVA+EL DFY
Sbjct:   64 QHVTVRRVAILY-PVITTYILLWGSIREPFMKKILGDDEYLWSYTKGFSELPSPAQLKASLAEQLSVEQLRDEFRQYIKTKVAQELIDFY 152          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: A0A6H5JJQ0_9PHAE (RGS domain-containing protein n=3 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JJQ0_9PHAE)

HSP 1 Score: 89.0 bits (219), Expect = 2.510e-18
Identity = 46/93 (49.46%), Postives = 66/93 (70.97%), Query Frame = 1
Query:   67 LDSHHKI-RRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFY 342
            LD  H I RR+ ++Y  +    +L W  I EP+ + + +D+EYL S+TKGFS LP+PAQL++S  +QLS+EQLR EF ++ + KVA+EL DFY
Sbjct:  252 LDGQHVIVRRVAIVYPALTTHTVL-WGSIGEPVLKKWTRDEEYLWSFTKGFSDLPSPAQLKASLAEQLSVEQLRAEFRRYIETKVAQELVDFY 343          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: D7FTZ6_ECTSI (Similar to regulator of G-protein signaling 16 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTZ6_ECTSI)

HSP 1 Score: 87.4 bits (215), Expect = 7.140e-18
Identity = 42/91 (46.15%), Postives = 65/91 (71.43%), Query Frame = 1
Query:   70 DSHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFY 342
            + H  +RR+ ++Y  V+++  L W  + EP+ + F  D+ YL S+TKGFS +P+PAQL++S  +QLS+EQLR EF ++ + KVA+EL DFY
Sbjct:  210 NQHVLVRRVAIVY-PVLSTYTLLWGSVGEPVVKKFTGDEGYLWSFTKGFSEMPSPAQLKASLAEQLSVEQLRVEFRRYIETKVAQELVDFY 299          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: D7FTZ7_ECTSI (Similar to regulator of G-protein signaling 16 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTZ7_ECTSI)

HSP 1 Score: 85.9 bits (211), Expect = 3.180e-17
Identity = 43/91 (47.25%), Postives = 62/91 (68.13%), Query Frame = 1
Query:   70 DSHHKIRRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFY 342
            + H  +RR+ ++Y  V+ +  L W  I EP+ R    D EYL S+TKGFS +P+PAQL++S  +QLS++QLR EF K+ +  VA+EL DFY
Sbjct:  186 NQHVYVRRVAIVY-PVLTTYTLLWGSIGEPIKRKLAGDTEYLYSFTKGFSEMPSPAQLKASLAEQLSVQQLRVEFRKYVETMVAQELVDFY 275          
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Match: A0A6H5KAT5_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KAT5_9PHAE)

HSP 1 Score: 83.6 bits (205), Expect = 2.510e-16
Identity = 44/85 (51.76%), Postives = 58/85 (68.24%), Query Frame = 1
Query:   88 RRIWVMYHNVIASLILFWPPIFEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEKFADAKVARELPDFY 342
            RR+ V Y  ++ + IL W  I EP       DDEYL SYTKGF+ LP+PAQL++S  +QLS++QLR EF ++   KVA+EL DFY
Sbjct:  222 RRVSVFY-PLLTTYILLWGSIREPFMMKILGDDEYLWSYTKGFAQLPSPAQLKASLAEQLSVDQLRDEFRRYIKTKVAQELIDFY 305          
The following BLAST results are available for this feature:
BLAST of mRNA_D-dudresnayi_contig10495.543.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 20
Match NameE-valueIdentityDescription
D7G0B4_ECTSI6.130e-4564.66RGS domain-containing protein n=2 Tax=Ectocarpus T... [more]
D7G7J3_ECTSI2.910e-4367.24RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A6H5K1F7_9PHAE1.540e-3746.82Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LI44_ECTSI8.320e-2046.15Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KDM3_9PHAE1.620e-1953.33RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FIS3_ECTSI2.040e-1953.33Similar to retinally abundant regulator of G-prote... [more]
A0A6H5JJQ0_9PHAE2.510e-1849.46RGS domain-containing protein n=3 Tax=Ectocarpus T... [more]
D7FTZ6_ECTSI7.140e-1846.15Similar to regulator of G-protein signaling 16 n=1... [more]
D7FTZ7_ECTSI3.180e-1747.25Similar to regulator of G-protein signaling 16 n=1... [more]
A0A6H5KAT5_9PHAE2.510e-1651.76RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-dudresnayi_contig10495contigD-dudresnayi_contig10495:436..2111 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Desmarestia dudresnayi DdudBR16 monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score163.3
Seed ortholog evalue6.8e-38
Seed eggNOG ortholog2880.D7G0B4
Preferred nameRGS1
KEGG koko:K16449
GOsGO:0001101,GO:0003674,GO:0004888,GO:0004930,GO:0005096,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005768,GO:0005886,GO:0006950,GO:0007154,GO:0007165,GO:0007186,GO:0008047,GO:0008150,GO:0009414,GO:0009415,GO:0009628,GO:0009719,GO:0009725,GO:0009737,GO:0009743,GO:0009746,GO:0009749,GO:0009756,GO:0009987,GO:0010008,GO:0010033,GO:0010035,GO:0010182,GO:0012505,GO:0016020,GO:0023052,GO:0030234,GO:0030695,GO:0031090,GO:0031410,GO:0031982,GO:0033993,GO:0034284,GO:0038023,GO:0042127,GO:0042221,GO:0043085,GO:0043087,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043547,GO:0044093,GO:0044422,GO:0044424,GO:0044433,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051336,GO:0051345,GO:0051716,GO:0060089,GO:0060589,GO:0065007,GO:0065009,GO:0070887,GO:0071310,GO:0071322,GO:0071944,GO:0097305,GO:0097708,GO:0098588,GO:0098772,GO:0098805,GO:1901700,GO:1901701
EggNOG free text desc.Regulator of G protein signaling domain
EggNOG OGs2CNEE@1,2QVMV@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000
Hectar predicted targeting categorysignal peptide
Exons3
Model size356
Cds size354
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-dudresnayi_contig10495.543.1prot_D-dudresnayi_contig10495.543.1Desmarestia dudresnayi DdudBR16 monoicouspolypeptideD-dudresnayi_contig10495 436..2111 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927551.7608678-CDS-D-dudresnayi_contig10495:435..5491622927551.7608678-CDS-D-dudresnayi_contig10495:435..549Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10495 436..549 -
1690556803.8599966-CDS-D-dudresnayi_contig10495:435..5491690556803.8599966-CDS-D-dudresnayi_contig10495:435..549Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10495 436..549 -
1622927551.77949-CDS-D-dudresnayi_contig10495:1248..13681622927551.77949-CDS-D-dudresnayi_contig10495:1248..1368Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10495 1249..1368 -
1690556803.8716235-CDS-D-dudresnayi_contig10495:1248..13681690556803.8716235-CDS-D-dudresnayi_contig10495:1248..1368Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10495 1249..1368 -
1622927551.792301-CDS-D-dudresnayi_contig10495:1991..21111622927551.792301-CDS-D-dudresnayi_contig10495:1991..2111Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10495 1992..2111 -
1690556803.8806798-CDS-D-dudresnayi_contig10495:1991..21111690556803.8806798-CDS-D-dudresnayi_contig10495:1991..2111Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10495 1992..2111 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-dudresnayi_contig10495.543.1

>prot_D-dudresnayi_contig10495.543.1 ID=prot_D-dudresnayi_contig10495.543.1|Name=mRNA_D-dudresnayi_contig10495.543.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=polypeptide|length=118bp
QVVQLLFLLITGTVVLPLQDVWLDSHHKIRRIWVMYHNVIASLILFWPPI
FEPMYRFFKQDDEYLQSYTKGFSTLPTPAQLRSSFKDQLSLEQLRREFEK
FADAKVARELPDFYKVG*
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mRNA from alignment at D-dudresnayi_contig10495:436..2111-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-dudresnayi_contig10495.543.1 ID=mRNA_D-dudresnayi_contig10495.543.1|Name=mRNA_D-dudresnayi_contig10495.543.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=mRNA|length=1676bp|location=Sequence derived from alignment at D-dudresnayi_contig10495:436..2111- (Desmarestia dudresnayi DdudBR16 monoicous)
CAGGTGGTGCAGCTGTTGTTTTTGCTCATCACGGGTACTGTGGTGCTTCC TCTTCAAGACGTCTGGCTTGACAGCCACCACAAAATACGGAGGATTTGGG TCATGTACCACAACGTTATCGTGAGCGTTATAACTAACCGGATGGTGGTT ATGTTTCGTGTTTGATGTTCGCGCGCAAAACGCGCGAGAAACAAGCTGTC ACACGATGCGCCCAAAATGTCAAGGATCTCAAGAATGTGTAAAAACTTGA TTCAACATATTCGCACGCTATTCTTATTATTTTTTACTTCAAATAGATAG AATCAGCCGTGACGTGCCAAATTGGTTCGGGGGGCCAGGGGATAATCGTC AAACTCATTGTGTGATACTTCGGTCGCGCAGAGTCTATCGGTCCTCAGAT AAGGTGTAACGCAGTGATTCCCCTGGGTTGCCCCGGGCTCTACAGGATAA TGGCGAGAAGGGAAATTTGGTTCTCTGTTATCATCTCTGTGCACTATAAT AGACGTACAGCCTTTCTGAGGAGAACACGAAGAGCAAAATAGATCGAATC AGACCAAAATTCTAGCCACGTAAGACAATACACATCATCACACTATCGCG CTGTATCGACAGTGTTGTCGTTGTAGCCGGCCGGCGTTTCATCCATCCCC TTCCGTATTCTCCTCTTGCTTGCTTATTGGCTGTTTGTATTTTTGCGTGT CGTATATGTGGGGGGTGCGTGCGTGTTGTGTGTTTTGTTGCAGGCGTCGC TGATCCTCTTTTGGCCCCCTATTTTCGAGCCCATGTATCGATTCTTCAAG CAAGACGACGAGTATCTGCAAAGCTACACGAAGGGATTTTCGACTCTTCC TACGCCGGCTCAGGTGAGCTAAATATCGTATTAAATAGTAATAACACGAA CAAGGTACTGTACTGTCGTTGTACTAACGATTGTTCTACAAAGTCGATGC TTTTGACGAAACGTCAACTTAATCAAGTACTTCGTATAACCTTCGTGGCG CTTGTGAAATACGCGTAGTGTATTGGATGCAGGGCAACCGCTGAATATAT TTTTTTGTGTGTGTATTCGTGACGACCGTGGTGGTGGATAACGGGGGGGG GGGGGGGGGGGGGGGTGCGCACGATAAATAAAAATCCGTGTTTTTAATGC AATACTACTGTTGTAAGTGCGCCGTTGGGGGGCTAACGAATGTTTTTCCC TCCCTATATCCGTGAGCCTACTTTACAAACAACGGTTGAAGTTGTTCGAG GGATAGTTAGGCTGTTCGTTTTATTTTACTAATAATAGTATACTAACGAG GGCTGGTAAGAACAGTTCGGAACGATTTCTAGAAGTAACCCTTATCCTAC AACATAACGACCACACCATACTAACAGCAAGTAAAAAATATAAATAGAGC CAGTTCCCACAACACGTTGTAAAAAGTCTAAATACTGGTATCCGTCCATC CCGCTCATGTGATTTTCGTGTTTTGTTTCGTTTTTTTGTTGTCTTCTGTT TTTTTTTTTTTGTGTCTGTGTGTGTGTGTGTGTGTGTTTTGCCGTTGCCG CGCGTTCGATAGTTGCGGTCATCTTTCAAGGATCAGCTATCGCTGGAGCA GCTGCGGCGAGAATTCGAAAAATTCGCGGACGCTAAGGTGGCGAGAGAAC TGCCCGATTTTTACAAGGTGGGATGA
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Coding sequence (CDS) from alignment at D-dudresnayi_contig10495:436..2111-

>mRNA_D-dudresnayi_contig10495.543.1 ID=mRNA_D-dudresnayi_contig10495.543.1|Name=mRNA_D-dudresnayi_contig10495.543.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=CDS|length=708bp|location=Sequence derived from alignment at D-dudresnayi_contig10495:436..2111- (Desmarestia dudresnayi DdudBR16 monoicous)
CAGGTGGTGCAGCTGTTGTTTTTGCTCATCACGGGTACTGTGGTGCTTCC
TCTTCAAGACGTCTGGCTTGACAGCCACCACAAAATACGGAGGATTTGGG
TCATGTACCACAACGTTATCCAGGTGGTGCAGCTGTTGTTTTTGCTCATC
ACGGGTACTGTGGTGCTTCCTCTTCAAGACGTCTGGCTTGACAGCCACCA
CAAAATACGGAGGATTTGGGTCATGTACCACAACGTTATCGCGTCGCTGA
TCCTCTTTTGGCCCCCTATTTTCGAGCCCATGTATCGATTCTTCAAGCAA
GACGACGAGTATCTGCAAAGCTACACGAAGGGATTTTCGACTCTTCCTAC
GCCGGCTCAGGCGTCGCTGATCCTCTTTTGGCCCCCTATTTTCGAGCCCA
TGTATCGATTCTTCAAGCAAGACGACGAGTATCTGCAAAGCTACACGAAG
GGATTTTCGACTCTTCCTACGCCGGCTCAGTTGCGGTCATCTTTCAAGGA
TCAGCTATCGCTGGAGCAGCTGCGGCGAGAATTCGAAAAATTCGCGGACG
CTAAGGTGGCGAGAGAACTGCCCGATTTTTACAAGGTGGGATGATTGCGG
TCATCTTTCAAGGATCAGCTATCGCTGGAGCAGCTGCGGCGAGAATTCGA
AAAATTCGCGGACGCTAAGGTGGCGAGAGAACTGCCCGATTTTTACAAGG
TGGGATGA
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