prot_C-tenellus_contig10442.1.1 (polypeptide) Choristocarpus tenellus KU2346

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview


Name	mRNA_C-tenellus_contig10442.1.1
Unique Name	prot_C-tenellus_contig10442.1.1
Type	polypeptide
Organism	Choristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Sequence length	335

Homology

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: D7FNM4_ECTSI (Mitogen-activated protein kinase kinase (MAP2K) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNM4_ECTSI)

HSP 1 Score: 207 bits (528), Expect = 6.200e-57
Identity = 134/336 (39.88%), Postives = 176/336 (52.38%), Query Frame = 0

Query:    1 DEDCNYNGELSPRFKKPTPWGNLTVHTTPVHEDSYRFTQSGTIWLNGFNTAIRNSGLAVAPGKCSWKVDQDALKGVGDVKCSE-------SDFLGEDGSKKNCGGNASVEQATIPVE-RAHVGTPMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKLG 328
            DE C+    L P    P    +L+++T      SYR+T  G I L+GF + I  SGL  A  +         +        S+       +D L + G +    G ++V  A   V     V TPM ER+VLLG+VG+G TGVVY+AFDL+ L  VA+K+IPVN++ KRRQ+V E+S+LY+ L G  G R+                                                         ID FAT  +ST+SLVVE+MDGGSLQDLVD+GGC DE  L Q+A+Q L+GL FLHS   IHRDIKPAN+LLN RGE+K++DFG +R LG
Sbjct:  277 DESCSTG--LEPSTSSPRHSKSLSLNTALGTAASYRYTPKGEILLSGFQSPIPQSGLMAAAWQAEGTQGDAVVSSAAAATISDGRDANLLADVLNDAGGRAATVGGSTVAAAAAEVAIGTMVLTPMIERVVLLGRVGEGETGVVYRAFDLLDLSLVAVKMIPVNDQKKRRQLVHEVSSLYDRL-GMRGQRRRR------------------------------------------------------LIDVFATTSNSTVSLVVEYMDGGSLQDLVDAGGCHDERKLGQIALQALRGLAFLHSSNLIHRDIKPANVLLNRRGELKIADFGLARTLG 555

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: A0A2R5GRK5_9STRA (Mitogen-activated protein kinase kinase 3 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GRK5_9STRA)

HSP 1 Score: 152 bits (384), Expect = 3.390e-38
Identity = 87/206 (42.23%), Postives = 120/206 (58.25%), Query Frame = 0

Query:  122 LVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKL 327
            LV++  +GQGA G+VYKA     L  VA+K IPV + +KR QM++EL ALYE             N   +GY   +   +             P  C                  +V F DAF   E + +++VVE+MDGGSLQD++D GGC++E+ L+Q+A + L+GL F+H R QIHRDIKP+N+L+NHRGEVK+SDFG  R+L
Sbjct:  197 LVMMAALGQGAGGIVYKAVHRPTLRLVAVKTIPVYDPTKRSQMLKELKALYE-------------NMAPIGYRDKAPLLDTST----------PAPCP----------------FIVTFHDAFINPEMANVAMVVEYMDGGSLQDIIDGGGCENEDILSQIAYRVLRGLQFIHERHQIHRDIKPSNLLINHRGEVKISDFGIVREL 363

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: A0A7S2PQG1_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2PQG1_9STRA)

HSP 1 Score: 151 bits (382), Expect = 3.150e-37
Identity = 106/331 (32.02%), Postives = 152/331 (45.92%), Query Frame = 0

Query:    1 DEDCNYNGELSPRFKKPTPWGNLTVHTTPVHEDSYRFTQSGTIWLNGFNTAIRNSGLAVAPGKCSWKVDQDALKGVGDVKCSESDFLGEDGSKKNCGGNASVEQATIPVERAHVGTPMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLH----GTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKL 327
            D+   ++  L  R K P    +L  H     + SY FT SGT+W++GF  AI N G+         K+ Q  L  +                                         M +RLV L K+G+GA+G V+KA DL  L  VA+K+I + +K KRRQ+V EL+ L+E L      T+G+ ++NG                                               ++ +V+ IDAF+   D ++ +  E+MDGGSLQD+VD G C +E  L+ +A Q L G+  LH   Q+HRDIKPANIL++ RG+VK+ D G SR L
Sbjct:  281 DDSLAFSSSL--RIKPPKI--HLPCHHHFGRKGSYTFTNSGTLWVDGFGKAITNRGI---------KLGQSPLGSIA----------------------------------------MRDRLVFLCKLGEGASGTVFKALDLCSLKLVAVKIISIADKRKRRQIVSELANLHELLRDSRVATSGDFELNG-----------------------------------------------EDCIVELIDAFSDANDKSVGICFEYMDGGSLQDIVDKGECANESILSGIAYQCLLGIGLLHRNNQVHRDIKPANILISTRGQVKIGDLGISRHL 511

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: A0A7S2LQJ8_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2LQJ8_9STRA)

HSP 1 Score: 151 bits (382), Expect = 4.660e-37
Identity = 106/331 (32.02%), Postives = 152/331 (45.92%), Query Frame = 0

Query:    1 DEDCNYNGELSPRFKKPTPWGNLTVHTTPVHEDSYRFTQSGTIWLNGFNTAIRNSGLAVAPGKCSWKVDQDALKGVGDVKCSESDFLGEDGSKKNCGGNASVEQATIPVERAHVGTPMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLH----GTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKL 327
            D+   ++  L  R K P    +L  H     + SY FT SGT+W++GF  AI N G+         K+ Q  L  +                                         M +RLV L K+G+GA+G V+KA DL  L  VA+K+I + +K KRRQ+V EL+ L+E L      T+G+ ++NG                                               ++ +V+ IDAF+   D ++ +  E+MDGGSLQD+VD G C +E  L+ +A Q L G+  LH   Q+HRDIKPANIL++ RG+VK+ D G SR L
Sbjct:  536 DDSLAFSSSL--RIKPPKI--HLPCHHHFGRKGSYTFTNSGTLWVDGFGKAITNRGI---------KLGQSPLGSIA----------------------------------------MRDRLVFLCKLGEGASGTVFKALDLCSLKLVAVKIISIADKRKRRQIVSELANLHELLRDSRVATSGDFELNG-----------------------------------------------EDCIVELIDAFSDANDKSVGICFEYMDGGSLQDIVDKGECANESILSGIAYQCLLGIGLLHRNNQVHRDIKPANILISTRGQVKIGDLGISRHL 766

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: A0A7R9UB23_9STRA (Hypothetical protein n=2 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UB23_9STRA)

HSP 1 Score: 149 bits (376), Expect = 2.220e-36
Identity = 86/210 (40.95%), Postives = 120/210 (57.14%), Query Frame = 0

Query:  118 MHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKL 327
            + + LV LG +G+G +G V+KA  +  L  VA+K IPV   ++R+QMV+EL  LYE+L                                      P    KG+   +   SK      +V F DAF T  D  +++VVE+MDGGSLQD+V +GGCQDE+ LA +  Q L+GL FLHSR+++HRDIKP+N+L+NH G+VK  DFG +R+L
Sbjct:  401 IQDSLVTLGLLGRGTSGTVHKALHVPTLRLVAVKEIPVFEATQRQQMVRELHTLYENLA-------------------------------------PLDDSKGDTTPTRPSSKACPH--IVTFYDAFMTTSDCVVAIVVEYMDGGSLQDIVKAGGCQDEQILASIGFQMLRGLAFLHSRRKMHRDIKPSNVLINHLGDVKYCDFGLAREL 571

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: K8YSV9_NANGC (Mitogen-activated protein kinase kinase 1 n=3 Tax=Monodopsidaceae TaxID=425072 RepID=K8YSV9_NANGC)

HSP 1 Score: 145 bits (367), Expect = 2.230e-35
Identity = 98/239 (41.00%), Postives = 127/239 (53.14%), Query Frame = 0

Query:  115 GTPMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESL----------------------------HGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSR 325
            G  +H+ LV LG +G+GA G V++   +  L  VA+K IPV  + +R QMVQEL +LY +L                                G   I G T              Q+    GA +  P+G  G D  +       G   +V F DA+A   + T+S+V EFMD GSLQD+VD+GGC DE  LA MA + L GL +LH+R+QIHRDIKPAN+LLNHRGEVK+SDFG  R
Sbjct:  162 GQGLHDCLVPLGVLGRGAGGSVWQGIHVPSLRLVAVKTIPVFEEGRRLQMVQELRSLYWNLAPFLPPSSSLAPSHSPPRTTPASPPPFPSAADGPGPIGG-TXXXXXXXLVGEPSGQVLIGTGAHRGSPVG--GMDAGASRGGG--GCPQLVSFYDAYANPREGTVSIVQEFMDAGSLQDVVDAGGCADEAVLAHMAREVLLGLAYLHARRQIHRDIKPANLLLNHRGEVKISDFGLVR 395

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: F0YL60_AURAN (Protein kinase domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YL60_AURAN)

HSP 1 Score: 135 bits (341), Expect = 1.160e-34
Identity = 86/208 (41.35%), Postives = 115/208 (55.29%), Query Frame = 0

Query:  120 ERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHS-RQQIHRDIKPANILLNHRGEVKLSDFGTSRK 326
            + LV LG++G GA   VYK   L  L  VA K I V++  K RQ+V+EL  LY +                   S S  +Q V I                   L   +     Q+ +VDF DAF  +++  +S++VE+MDGGSLQD+VDSGGC  E  LA +A + L GL +LHS R  IHRDIKPAN+L+NH G+VK+ DFGT+R+
Sbjct:    1 DELVSLGELGYGACSTVYKKLHLPSLRIVAEKKISVSDAQKCRQIVRELQTLYLA-------------------SISQSAQTVHIWVSD---------------LGPFKCPFSVQKYIVDFYDAFIDLDNGQLSVLVEYMDGGSLQDVVDSGGCPHETVLANIAQRILNGLVYLHSIRNCIHRDIKPANLLINHHGDVKIGDFGTARE 174

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: A0A7S1UEU1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UEU1_9STRA)

HSP 1 Score: 136 bits (343), Expect = 5.560e-32
Identity = 83/211 (39.34%), Postives = 121/211 (57.35%), Query Frame = 0

Query:  117 PMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKL 327
            P+ +RLV+L K+G+GA+  V+K +DL     VA+KVIP+ N SKR Q + EL AL  SL                        QE+        ++  P     ED   ++ +   G   ++ F DAF   E++TI+L +E MDGGSL+D+VD+GG + E T+A +A Q L GL  LH + Q+HRD+KPANIL++ +G V++SDFG +R  
Sbjct:  354 PLTDRLVVLSKLGEGASAHVFKCYDLEFQEIVAVKVIPIFNVSKRGQFMHELEALRSSLE----------------------DQELHESPQSYVEKLEPT----ED--GQLPAVEAGIPFLLRFKDAFCNREEATIALTMEHMDGGSLEDIVDTGGLRFEPTIASIAYQVLLGLRLLHHKGQLHRDLKPANILMSRKGHVRISDFGVARNF 536

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: A0A7S1UDR3_9STRA (Hypothetical protein n=3 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UDR3_9STRA)

HSP 1 Score: 136 bits (343), Expect = 6.280e-32
Identity = 83/211 (39.34%), Postives = 121/211 (57.35%), Query Frame = 0

Query:  117 PMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKL 327
            P+ +RLV+L K+G+GA+  V+K +DL     VA+KVIP+ N SKR Q + EL AL  SL                        QE+        ++  P     ED   ++ +   G   ++ F DAF   E++TI+L +E MDGGSL+D+VD+GG + E T+A +A Q L GL  LH + Q+HRD+KPANIL++ +G V++SDFG +R  
Sbjct:  438 PLTDRLVVLSKLGEGASAHVFKCYDLEFQEIVAVKVIPIFNVSKRGQFMHELEALRSSLE----------------------DQELHESPQSYVEKLEPT----ED--GQLPAVEAGIPFLLRFKDAFCNREEATIALTMEHMDGGSLEDIVDTGGLRFEPTIASIAYQVLLGLRLLHHKGQLHRDLKPANILMSRKGHVRISDFGVARNF 620

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BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Match: F0Y7H4_AURAN (Protein kinase domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y7H4_AURAN)

HSP 1 Score: 129 bits (325), Expect = 6.390e-32
Identity = 83/203 (40.89%), Postives = 106/203 (52.22%), Query Frame = 0

Query:  128 VGQGATGVVYKAFDLMRLHHVAIKVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQEVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDSTISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIHRDIKPANILLNHRGEVKLSDFGTSRKLGGE 330
            +G+GA+G V+KA DL+ L  VAIK I V    KRRQ+V EL ALY                                    GA Q P     G D              VV F DAFA        L+VE++DGGSLQDLVD GG  DE  LA++A Q  +GL +LH+R  +HRD+KPAN+LL+  G +K+SDFG +R+L G+
Sbjct:    3 LGEGASGAVHKALDLVDLRFVAIKAISVGAAPKRRQLVAELLALY------------------------------------GAFQGPDAEAAGGDA-----------RHVVSFYDAFAHAPTHCAWLMVEYLDGGSLQDLVDRGGTTDEALLARLAFQIARGLRYLHARGFVHRDVKPANMLLSRAGALKISDFGIARRLDGD 158

The following BLAST results are available for this feature:

BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot

BLAST of mRNA_C-tenellus_contig10442.1.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
D7FNM4_ECTSI	6.200e-57	39.88	Mitogen-activated protein kinase kinase (MAP2K) n=...	[more]
A0A2R5GRK5_9STRA	3.390e-38	42.23	Mitogen-activated protein kinase kinase 3 n=1 Tax=...	[more]
A0A7S2PQG1_9STRA	3.150e-37	32.02	Hypothetical protein n=1 Tax=Leptocylindrus danicu...	[more]
A0A7S2LQJ8_9STRA	4.660e-37	32.02	Hypothetical protein n=1 Tax=Leptocylindrus danicu...	[more]
A0A7R9UB23_9STRA	2.220e-36	40.95	Hypothetical protein n=2 Tax=Pinguiococcus pyrenoi...	[more]
K8YSV9_NANGC	2.230e-35	41.00	Mitogen-activated protein kinase kinase 1 n=3 Tax=...	[more]
F0YL60_AURAN	1.160e-34	41.35	Protein kinase domain-containing protein (Fragment...	[more]
A0A7S1UEU1_9STRA	5.560e-32	39.34	Hypothetical protein (Fragment) n=1 Tax=Phaeomonas...	[more]
A0A7S1UDR3_9STRA	6.280e-32	39.34	Hypothetical protein n=3 Tax=Phaeomonas parva TaxI...	[more]
F0Y7H4_AURAN	6.390e-32	40.89	Protein kinase domain-containing protein (Fragment...	[more]

Pages

InterPro

Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 122..335 e-value: 6.1E-9 score: -7.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 200..331 e-value: 1.4E-25 score: 90.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 122..335 score: 27.001
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 109..179 e-value: 2.1E-8 score: 36.3
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 193..335 e-value: 3.9E-29 score: 103.6
None	No IPR available	PIRSR	PIRSR037921-2	PIRSR037921-2	coord: 121..171 e-value: 110.0 score: -0.4
None	No IPR available	PANTHER	PTHR45832:SF6	SUBFAMILY NOT NAMED	coord: 118..327
None	No IPR available	PANTHER	PTHR45832	FAMILY NOT NAMED	coord: 118..327
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 128..151
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 115..329

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
C-tenellus_contig10442	contig	C-tenellus_contig10442:4285..5385 +

Analyses

This polypeptide is derived from or has results from the following analyses


Analysis Name	Date Performed
InterProScan on OGS1.0	2022-09-29
Diamond blastp: OGS1.0 vs UniRef90	2022-09-16
Choristocarpus tenellus KU2346 OGS1.0	2022-07-08

Relationships

This polypeptide derives from the following mRNA feature(s):


Feature Name	Unique Name	Species	Type	Position
mRNA_C-tenellus_contig10442.1.1	mRNA_C-tenellus_contig10442.1.1	Choristocarpus tenellus KU2346	mRNA	C-tenellus_contig10442 4285..5385 +

Sequences

The following sequences are available for this feature:

polypeptide sequence

polypeptide sequence

>prot_C-tenellus_contig10442.1.1 ID=prot_C-tenellus_contig10442.1.1|Name=mRNA_C-tenellus_contig10442.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=335bp
DEDCNYNGELSPRFKKPTPWGNLTVHTTPVHEDSYRFTQSGTIWLNGFNT
AIRNSGLAVAPGKCSWKVDQDALKGVGDVKCSESDFLGEDGSKKNCGGNA
SVEQATIPVERAHVGTPMHERLVLLGKVGQGATGVVYKAFDLMRLHHVAI
KVIPVNNKSKRRQMVQELSALYESLHGTTGNRKINGNTLQLGYSTSSRSQ
EVQIQACKGAKQHPPLGCKGEDFLSEIESKTLGQESVVDFIDAFATIEDS
TISLVVEFMDGGSLQDLVDSGGCQDEETLAQMAIQGLKGLFFLHSRQQIH
RDIKPANILLNHRGEVKLSDFGTSRKLGGEEDSKE

Annotated Terms

The following terms have been associated with this polypeptide:

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR017441	Protein_kinase_ATP_BS
IPR011009	Kinase-like_dom_sf