mRNA_C-tenellus_contig7970.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig7970.1.1
Unique NamemRNA_C-tenellus_contig7970.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: D7FLN1_ECTSI (Asparagine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FLN1_ECTSI)

HSP 1 Score: 168 bits (425), Expect = 3.460e-45
Identity = 90/182 (49.45%), Postives = 121/182 (66.48%), Query Frame = 1
Query:    1 MLRRFAGVDLAVRTGVSQGDLEREAPAAKSLLLKITRPGGVPDV----FLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEAIALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLATVAEPLLKGASGAAAAASTGGVRLLSSKDIEGLAHVM 534
            ++ R AG+DL ++TG+S+ ++E  AP AK L+LK+ RPG    V     +TQSNA+LR +A  +  S LYGR+EFESA  DQWLETCW E EVP +A+A A+GN AVI E+K HLL+FLA AN  L+  T+L  ER TIAD+ LA  A  L++G+         GG   L ++D+ GL HV+
Sbjct:   37 VVARMAGIDLDLKTGMSEEEMEAFAPLAKGLVLKVCRPGQAAPVGGEEAVTQSNAILRTLAMAKADSSLYGRSEFESAQADQWLETCWKELEVPAQALAAASGNQAVIAEAKRHLLSFLATANATLSTSTFLVGERTTIADVGLACAARDLIEGSV-------AGGAASLGARDLAGLPHVL 211          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: A0A835YW11_9STRA (Asparagine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YW11_9STRA)

HSP 1 Score: 122 bits (306), Expect = 5.100e-29
Identity = 74/173 (42.77%), Postives = 103/173 (59.54%), Query Frame = 1
Query:   10 RFAGVDLAVRTGVSQGDLEREAPAAKSLLLKITRPGGVPDVFLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEAIALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLATVAEPLLKGASGAAAAASTGGVRLLSSKDIEGLAH 528
            R AG+ +A++T  ++  +  +APAAK+L L+ITRP      ++TQSNA++R +     TS LYG TEF  + VD WLE CW E E P+  +  A G+  VI E+K  +L  L   N  LA +TYL   R+T ADI+LA  A+P+L    GAAA+       +LS KD+  L H
Sbjct:   39 RAAGIPVALKTCSAEA-IGEQAPAAKALALRITRPTSAHPSYITQSNAIVRTLIDASETSGLYGDTEFARSQVDSWLELCWLELETPLAVLQQAKGHGDVIAEAKKLILHALTAVNAALAARTYLVGNRLTAADIALACAAQPIL----GAAAS-------VLSGKDLATLPH 199          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: A0A7S2UT83_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UT83_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 2.940e-18
Identity = 57/145 (39.31%), Postives = 81/145 (55.86%), Query Frame = 1
Query:   10 RFAGVDLAVRTGVSQGDLEREAPAAKSLLL--KITRPGGVPDVFLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVE-----AIALAAGNSAVITESKHHLLAFLA-IANTRLAQKTYLGAERITIADISL 420
            + +GV LA + GV Q  L    P AK+++L  + T+        +TQSNA+LR IA L P + LYGR +F+SA VDQWLE  W+E EVP       A  L   + A    +K  LL  L  +    LA++T+L  +R ++AD+ L
Sbjct:   27 KLSGVKLAHQPGVQQSVLTSFLPHAKTVVLHDETTKRS------ITQSNAILRMIAQLNPAAELYGRDDFQSAQVDQWLEFAWNEVEVPAAVCLFPARGLLEKDDAAAAMAKQDLLTSLGHVLENHLAERTFLVGQRPSLADVGL 165          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: F0YK49_AURAN (Uncharacterized protein n=2 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YK49_AURAN)

HSP 1 Score: 80.1 bits (196), Expect = 2.170e-14
Identity = 52/146 (35.62%), Postives = 74/146 (50.68%), Query Frame = 1
Query:   13 FAGVDLAVRTGVSQGDLEREAPAAKSLLLKITRPGGVPDVFLTQ-----SNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEA-----IALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISL 420
            + GVD+ V       D + E  A     L ++  G VP +   Q     SNA+ RY+A LR  S +YGRT +ESAL+D W++ C HE E+P        I     N+A   ++K H+   L      L  KTY+  + IT+ADI+L
Sbjct:   23 YVGVDIDVP------DFDMEKTAKTPSFLAMSPMGKVPVLKTPQGAIFESNAIARYVARLRKDSEIYGRTFYESALIDSWVDFCAHELELPCTMWVYPIIGYMPFNAAASAKAKEHVTDALDSLERHLVDKTYIVGDAITLADITL 162          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: A0A8J2WRT9_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WRT9_9STRA)

HSP 1 Score: 77.0 bits (188), Expect = 2.580e-13
Identity = 51/148 (34.46%), Postives = 80/148 (54.05%), Query Frame = 1
Query:   13 FAGVDLAVRTGVSQGDLEREAPAAKSLLLKITRPGGVPDVFLTQ-----SNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEA-----IALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLAT 426
            +AGV++AV+      D++ +A A      K++  G VP +   Q     SNA+ RY+A LR  + LYGRT +ESA+VD W++ C HE E+P        I     +     ++K H+   L   +  L  KTY+  E +T+ADI++A+
Sbjct:   23 YAGVEIAVK------DVDAKAAA------KLSPTGKVPVLETPQGTIFESNAIARYVARLRSDADLYGRTFYESAVVDSWIDFCAHELELPCTLWVYPIIGYMPFSGPAYGKAKAHVSQALKTLDAHLLDKTYMVGEAMTLADITIAS 158          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: A0A7S0SSV9_9STRA (Hypothetical protein n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0SSV9_9STRA)

HSP 1 Score: 77.0 bits (188), Expect = 2.830e-13
Identity = 39/102 (38.24%), Postives = 62/102 (60.78%), Query Frame = 1
Query:  136 LTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEAI-----ALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLAT 426
            +T+SNA+ RYIA LRP   LYG+T ++SA++D W++   H+ E+P   +        A N  +  ++K+ L     + N  L +KTYL  E I++ADI++AT
Sbjct:   75 ITESNAIARYIARLRPDIGLYGKTFYDSAVIDSWIDYNSHDIELPATLLLYPILGYTAYNEVIANKAKNDLTKVFQLLNNYLKEKTYLVGESISLADITIAT 176          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: A0A7S2SN27_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SN27_9STRA)

HSP 1 Score: 75.1 bits (183), Expect = 4.360e-13
Identity = 41/108 (37.96%), Postives = 61/108 (56.48%), Query Frame = 1
Query:  133 FLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEAIALAA----GNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLATVAEPLL 444
            +LTQ+ A+ RY+A LRP   L G +E +   VDQW+E CW+E EV + A+ L +       AV       ++  L + +  LA KT++   R++IAD+ LA V   LL
Sbjct:   87 YLTQTKAIARYLAGLRPDLGLAGASEADEPFVDQWMEFCWYELEVLLGALLLPSTAEFDPKAVAQAVSTDIIKALQVLDAHLADKTFVAGSRLSIADVHLAVVTSELL 194          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: D7G6C4_ECTSI (Eukaryotic elongation factor-1 B gamma n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6C4_ECTSI)

HSP 1 Score: 72.4 bits (176), Expect = 1.090e-11
Identity = 39/106 (36.79%), Postives = 60/106 (56.60%), Query Frame = 1
Query:  124 PDVFLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVE-----AIALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLAT 426
            P   + +SNA+ RY+A +R  + LYG + FESA VD W++ C HE E+P        I     N A ++++K  L   L      L  +TYL  ++IT+ADI++A+
Sbjct:   59 PQGSIFESNAIARYVARMRRDTELYGVSFFESAQVDSWIDFCAHEIELPATIWCYPVIGYMPYNDAAVSKAKEDLKIALGALEKALLHRTYLVGDKITLADITVAS 164          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: W7TL25_9STRA (Elongation factor 1 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TL25_9STRA)

HSP 1 Score: 72.4 bits (176), Expect = 1.120e-11
Identity = 49/140 (35.00%), Postives = 76/140 (54.29%), Query Frame = 1
Query:   22 VDLAVRTGVSQGDLEREAPAAKSLLLKITRPGGVPDVFLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVE-----AIALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLAT 426
            +DLA +    + D+  ++PA K  +L+       P   L +SNA+ RYIA  R  + LYG + F+S LVD W++    E E+P              N+AV  ++K  L A + I +  LA KTYL  E++T+ADI++A+
Sbjct:   33 LDLAAK---KRTDVLAKSPAGKVPVLE------TPQGLLFESNAIARYIARARRDTDLYGVSFFDSGLVDAWIDFAAQEVELPATLWVYPVFGYLPHNAAVTEKAKADLGAAMEILDNHLADKTYLVGEKVTLADITVAS 163          
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Match: A0A2C6KYN3_9APIC (Elongation factor 1-gamma n=1 Tax=Cystoisospora suis TaxID=483139 RepID=A0A2C6KYN3_9APIC)

HSP 1 Score: 71.2 bits (173), Expect = 2.930e-11
Identity = 47/120 (39.17%), Postives = 64/120 (53.33%), Query Frame = 1
Query:   70 EAPAAKSLLLKITRPGGVPDVFLTQSNAVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEAIALAAGNSAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLATV 429
            ++P  +  LL+    GGV    L +SNA+ R++A LRP   LYG T  E   VD WL+    E E+P+    L  G   V   +K  +   L + NT L   TY+  ERITIAD+ LAT+
Sbjct:   90 KSPLGRLPLLESELGGGV---CLFESNAICRFLARLRPDKYLYGETLGEQGYVDMWLDFTTLEVEIPM--CCLVQGGK-VAERAKSDVSRALNVINTHLKTNTYMVGERITIADLCLATI 203          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig7970.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FLN1_ECTSI3.460e-4549.45Asparagine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2... [more]
A0A835YW11_9STRA5.100e-2942.77Asparagine--tRNA ligase n=1 Tax=Tribonema minus Ta... [more]
A0A7S2UT83_9STRA2.940e-1839.31Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
F0YK49_AURAN2.170e-1435.62Uncharacterized protein n=2 Tax=Aureococcus anopha... [more]
A0A8J2WRT9_9STRA2.580e-1334.46Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A7S0SSV9_9STRA2.830e-1338.24Hypothetical protein n=1 Tax=Chromulina nebulosa T... [more]
A0A7S2SN27_9STRA4.360e-1337.96Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
D7G6C4_ECTSI1.090e-1136.79Eukaryotic elongation factor-1 B gamma n=1 Tax=Ect... [more]
W7TL25_9STRA1.120e-1135.00Elongation factor 1 n=2 Tax=Monodopsidaceae TaxID=... [more]
A0A2C6KYN3_9APIC2.930e-1139.17Elongation factor 1-gamma n=1 Tax=Cystoisospora su... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig7970contigC-tenellus_contig7970:3..1180 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FLN1
Preferred nameNS2
PFAMsGST_C,GST_C_3,GST_N_3,WHEP-TRS,tRNA-synt_2,tRNA_anti-codon
Model size534
Max annot lvl2759|Eukaryota
KEGG rclassRC00004,RC00055,RC00069,RC00523,RC00840,RC00948,RC01704,RC01705,RC01706,RC01758,RC01759,RC01765,RC01767,RC01769,RC02243,RC02527,RC02939,RC02940,RC02942,RC02943,RC02944
KEGG koko:K00799,ko:K01872,ko:K01893,ko:K03233,ko:K10028
KEGG TC1.A.12.2.2,1.A.12.3.2
KEGG ReactionR03038,R03522,R03648,R07002,R07003,R07004,R07023,R07024,R07025,R07026,R07069,R07070,R07083,R07084,R07091,R07092,R07093,R07094,R07100,R07113,R07116,R08280,R09409,R11905
KEGG Pathwayko00480,ko00970,ko00980,ko00982,ko00983,ko01524,ko05134,ko05200,ko05204,ko05225,ko05418,map00480,map00970,map00980,map00982,map00983,map01524,map05134,map05200,map05204,map05225,map05418
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003006,GO:0003674,GO:0003676,GO:0003723,GO:0003746,GO:0003824,GO:0004364,GO:0004812,GO:0004816,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005829,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006414,GO:0006418,GO:0006421,GO:0006518,GO:0006520,GO:0006575,GO:0006725,GO:0006749,GO:0006790,GO:0006807,GO:0006950,GO:0006979,GO:0007275,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009266,GO:0009408,GO:0009507,GO:0009536,GO:0009628,GO:0009790,GO:0009791,GO:0009793,GO:0009987,GO:0010035,GO:0010038,GO:0010154,GO:0010467,GO:0016070,GO:0016740,GO:0016765,GO:0016874,GO:0016875,GO:0019538,GO:0019752,GO:0022414,GO:0032501,GO:0032502,GO:0034641,GO:0034645,GO:0034660,GO:0042221,GO:0043038,GO:0043039,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0046686,GO:0048316,GO:0048608,GO:0048731,GO:0048856,GO:0050896,GO:0051186,GO:0061458,GO:0071704,GO:0090304,GO:0097159,GO:0140098,GO:0140101,GO:1901360,GO:1901363,GO:1901564,GO:1901566,GO:1901576
Exons2
Evalue5.68e-46
EggNOG OGsCOG0017@1|root,COG0625@1|root,KOG0554@2759|Eukaryota,KOG0867@2759|Eukaryota
EC2.5.1.18,4.1.1.84,6.1.1.22,6.1.1.7
DescriptionBelongs to the GST superfamily
Cds size534
COG categoryO
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko02000,ko03012,ko03016,ko03019
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790072.8053637-CDS-C-tenellus_contig7970:2..3111680790072.8053637-CDS-C-tenellus_contig7970:2..311Choristocarpus tenellus KU2346CDSC-tenellus_contig7970 3..311 -
1680790072.8184204-CDS-C-tenellus_contig7970:955..11801680790072.8184204-CDS-C-tenellus_contig7970:955..1180Choristocarpus tenellus KU2346CDSC-tenellus_contig7970 956..1180 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig7970.1.1prot_C-tenellus_contig7970.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig7970 3..1180 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig7970.1.1

>prot_C-tenellus_contig7970.1.1 ID=prot_C-tenellus_contig7970.1.1|Name=mRNA_C-tenellus_contig7970.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=178bp
MLRRFAGVDLAVRTGVSQGDLEREAPAAKSLLLKITRPGGVPDVFLTQSN
AVLRYIATLRPTSLLYGRTEFESALVDQWLETCWHEFEVPVEAIALAAGN
SAVITESKHHLLAFLAIANTRLAQKTYLGAERITIADISLATVAEPLLKG
ASGAAAAASTGGVRLLSSKDIEGLAHVM
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mRNA from alignment at C-tenellus_contig7970:3..1180-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig7970.1.1 ID=mRNA_C-tenellus_contig7970.1.1|Name=mRNA_C-tenellus_contig7970.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=1178bp|location=Sequence derived from alignment at C-tenellus_contig7970:3..1180- (Choristocarpus tenellus KU2346)
ATGCTGCGAAGGTTTGCTGGTGTAGACCTGGCTGTGAGGACTGGGGTGAG TCAAGGAGATCTGGAGCGGGAAGCCCCAGCTGCTAAAAGCCTACTACTCA AGATCACCAGGCCTGGGGGGGTACCAGACGTGTTCCTTACACAGAGCAAT GCCGTCTTGCGCTACATCGCCACGCTGCGACCTACATCCTTGCTCTATGG ACGCACTGAATTTGAATCTGCGTTGGTGAGTCTTGCTAGAGCTTCTCTAA ACCCCCACATACAACAGTGCCCTAACAGGATATATGTTCTTGTTATATCA ATCTAAGAATATTGTTGGCAATTCCCTTGGGGTCAATACCCGTCCAAATA CCGCCCCCCTTCATTTTTGTTTGGGTGCATGGAGTCTGCTCGTCTGTCCT CCTTGAATGCCATGTATACTGTATTCGGATATAACAATGAAGAAGTTTTT CCCATTCTCAGCATGTTAGCCAGAACCACCAGAATTCTACCTCTTAAAAC TTTCTCTAGCCTTGGGGTAATTGTGTTAGTACCAGTGACTGGAGCCTTAA CTAGGGTGCACCTACAGGTCGTTTTGATCACGTGGTGTAACCTGGGCATG CGGAGAGTGACAGCCCATTGCATTTGAGATTGCCCGTGCGATCTTAATTT GTGGGCACGGACGACGTTTCACCATATTTTAGGGTGCAATTGTTGGGGAC AACATAACACGTTCAATTGATTGCTTCAAATTTGTACTCTGCAGCGGGGT AGAAACCCCATTTGATTCAAGGAGTAAGTTCAAAATTGTTAGAATCTACA AGAGTCTCCTCTTATGCCCACCCCCATGTGTTCGTGTTCTGCCTCCCCCT CGGTCTCTCCTCACAATAGGTGGACCAATGGCTTGAAACTTGTTGGCACG AGTTTGAGGTCCCTGTAGAGGCTATAGCGCTTGCTGCTGGCAATTCGGCT GTGATCACCGAATCCAAGCACCACCTGCTGGCATTTCTAGCCATTGCAAA CACAAGGTTGGCCCAGAAAACATACTTGGGCGCAGAGAGGATAACTATTG CTGACATATCTCTAGCCACCGTTGCAGAGCCTTTACTCAAAGGGGCTTCA GGGGCAGCGGCAGCGGCCTCAACAGGGGGGGTGCGTCTGCTAAGTTCCAA GGATATTGAGGGGCTCGCACACGTAATG
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Coding sequence (CDS) from alignment at C-tenellus_contig7970:3..1180-

>mRNA_C-tenellus_contig7970.1.1 ID=mRNA_C-tenellus_contig7970.1.1|Name=mRNA_C-tenellus_contig7970.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=534bp|location=Sequence derived from alignment at C-tenellus_contig7970:3..1180- (Choristocarpus tenellus KU2346)
ATGCTGCGAAGGTTTGCTGGTGTAGACCTGGCTGTGAGGACTGGGGTGAG
TCAAGGAGATCTGGAGCGGGAAGCCCCAGCTGCTAAAAGCCTACTACTCA
AGATCACCAGGCCTGGGGGGGTACCAGACGTGTTCCTTACACAGAGCAAT
GCCGTCTTGCGCTACATCGCCACGCTGCGACCTACATCCTTGCTCTATGG
ACGCACTGAATTTGAATCTGCGTTGGTGGACCAATGGCTTGAAACTTGTT
GGCACGAGTTTGAGGTCCCTGTAGAGGCTATAGCGCTTGCTGCTGGCAAT
TCGGCTGTGATCACCGAATCCAAGCACCACCTGCTGGCATTTCTAGCCAT
TGCAAACACAAGGTTGGCCCAGAAAACATACTTGGGCGCAGAGAGGATAA
CTATTGCTGACATATCTCTAGCCACCGTTGCAGAGCCTTTACTCAAAGGG
GCTTCAGGGGCAGCGGCAGCGGCCTCAACAGGGGGGGTGCGTCTGCTAAG
TTCCAAGGATATTGAGGGGCTCGCACACGTAATG
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