mRNA_C-tenellus_contig7376.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig7376.1.1
Unique NamemRNA_C-tenellus_contig7376.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: A0A7J7K981_BUGNE (Glyco_trans_2-like domain-containing protein n=1 Tax=Bugula neritina TaxID=10212 RepID=A0A7J7K981_BUGNE)

HSP 1 Score: 110 bits (275), Expect = 8.250e-25
Identity = 59/128 (46.09%), Postives = 89/128 (69.53%), Query Frame = 2
Query:  494 TASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDD-GNAAVEGSP----VWSMPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDS 862
            T SVI+ F+NE+ + L RSV ++++RT+   L +IILVDD  N A  G      +  + KVR++R K+REG++RAR+ G++ A+G+VL FFD+HIE  EGWLEP++G I  + +T+  P+LD I  D+
Sbjct:   92 TTSVILIFHNEALSTLLRSVYSIIDRTDPKLLKEIILVDDFSNRAGLGQELDDYIKDLEKVRVLRLKKREGLIRARLAGAKVAKGQVLTFFDSHIECTEGWLEPMLGEIHRDKKTVPQPSLDDISHDT 219          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: A0A6P7VCC4_IXOSC (Polypeptide N-acetylgalactosaminyltransferase n=4 Tax=Ixodes TaxID=6944 RepID=A0A6P7VCC4_IXOSC)

HSP 1 Score: 113 bits (282), Expect = 1.890e-24
Identity = 63/130 (48.46%), Postives = 86/130 (66.15%), Query Frame = 2
Query:  494 TASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDGNAAVEGSPVWS------MPK-VRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDS 862
            TASV++ FYNE+ + L R+V T+L RT    L ++ILVDD +  V+  P  +      +P  VRL+RT+ REG++RARM G+R A GEVLVF D+H EV  GWLEPL+  IR N  T+  P +D+I  D+
Sbjct:  144 TASVVVCFYNEAWSTLLRTVHTVLGRTPRHLLHEVILVDDNSTQVDLGPQLAEYVSSQLPSHVRLIRTRDREGLIRARMFGARNASGEVLVFLDSHCEVNVGWLEPLLERIRANRATVTCPIIDIINADT 273          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: B7PEU0_IXOSC (Polypeptide N-acetylgalactosaminyltransferase n=1 Tax=Ixodes scapularis TaxID=6945 RepID=B7PEU0_IXOSC)

HSP 1 Score: 113 bits (282), Expect = 2.090e-24
Identity = 63/130 (48.46%), Postives = 86/130 (66.15%), Query Frame = 2
Query:  494 TASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDGNAAVEGSPVWS------MPK-VRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDS 862
            TASV++ FYNE+ + L R+V T+L RT    L ++ILVDD +  V+  P  +      +P  VRL+RT+ REG++RARM G+R A GEVLVF D+H EV  GWLEPL+  IR N  T+  P +D+I  D+
Sbjct:  190 TASVVVCFYNEAWSTLLRTVHTVLGRTPRHLLHEVILVDDNSTQVDLGPQLAEYVSSQLPSHVRLIRTRDREGLIRARMFGARNASGEVLVFLDSHCEVNVGWLEPLLERIRANRATVTCPIIDIINADT 319          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: A0A433SW88_ELYCH (Polypeptide N-acetylgalactosaminyltransferase n=1 Tax=Elysia chlorotica TaxID=188477 RepID=A0A433SW88_ELYCH)

HSP 1 Score: 111 bits (277), Expect = 8.320e-24
Identity = 57/129 (44.19%), Postives = 91/129 (70.54%), Query Frame = 2
Query:  494 TASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDGNAA------VEGSPVWSMPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDS 862
            TASVI+ FYNE+ + L R+V T++ER+    + +IILVDD +        ++     + P+V+L+RT +R+G++RARM+G+R A GEVL+F D+H EV  GWLEPL+  +RN+  T++VP +D++ +D+
Sbjct:  127 TASVIICFYNEAKSTLLRTVNTVVERSPAYLIHEIILVDDYSDLDFLKHDLQNHMQNNFPQVKLLRTSERQGLIRARMVGAREASGEVLLFLDSHCEVNVGWLEPLLFAVRNDPHTVSVPIIDIVNSDT 255          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: A0A2D0S4X9_ICTPU (Polypeptide N-acetylgalactosaminyltransferase n=7 Tax=Siluroidei TaxID=1489793 RepID=A0A2D0S4X9_ICTPU)

HSP 1 Score: 110 bits (276), Expect = 1.120e-23
Identity = 59/132 (44.70%), Postives = 87/132 (65.91%), Query Frame = 2
Query:  491 QTASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDGNAAVEGSPVWSM-------PKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDSV 865
            QTASV++ F+NE+ + L R+V ++L+RT    L +I+LVDD +   +      M        KV+LVR K+REG++R RMIG+ +A GEVLVF D+H EV E WL+PL+  I+ N RT+  P +D+I  D++
Sbjct:  131 QTASVVICFFNEAFSALLRTVHSVLDRTPSYLLHEIVLVDDNSELDDLKQDLDMYIHTNLPDKVKLVRNKRREGLIRGRMIGASHATGEVLVFLDSHCEVNEAWLQPLLSPIKENRRTVVCPVIDIISADTL 262          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: UPI0008F9DE1D (polypeptide N-acetylgalactosaminyltransferase 35A-like n=1 Tax=Bemisia tabaci TaxID=7038 RepID=UPI0008F9DE1D)

HSP 1 Score: 110 bits (275), Expect = 1.710e-23
Identity = 67/153 (43.79%), Postives = 93/153 (60.78%), Query Frame = 2
Query:  425 RAVGKKQPPVCNLHYAPRTSTPQTASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDD-----GNAAVEGSPVWS--MPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDS 862
            R++   + P+C     P  S   TAS+++ FYNE A  L+RSV ++LERT    L +IILVDD     G        V S  M KV+LVR KQREG++RAR++G+    G+VL+F D+HIEV  GW+EPL+  I  +  T+  P +DVI  D+
Sbjct:  149 RSINDTRHPLCRTQTYP--SDLPTASIVICFYNEHADTLKRSVHSILERTPERFLHEIILVDDFSDLPGLEEEVNQFVASKGMEKVKLVRAKQREGLIRARVLGAGKTSGQVLIFLDSHIEVNVGWIEPLLAAIAADSHTVVTPIIDVINADT 299          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: A0A2T7NXH9_POMCA (Polypeptide N-acetylgalactosaminyltransferase n=3 Tax=Pomacea canaliculata TaxID=400727 RepID=A0A2T7NXH9_POMCA)

HSP 1 Score: 109 bits (273), Expect = 1.740e-23
Identity = 59/145 (40.69%), Postives = 97/145 (66.90%), Query Frame = 2
Query:  419 STRAVGKKQPPVCNLHYAPRTSTPQTASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDD-GNAAVEGSPVWSMPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVI 850
            STR V   +P +C   +    S P T ++I++F+NE+ + L R+++++L R+    + +IILVDD  + A +G  + S+PKV+++R ++REG +R+R+ GS  A+G+VL F D+H EV +GWLEPL+ TI+ N  +I  P +D I
Sbjct:   79 STRPVPDARPILCKTFFPASPSLPST-TIIVTFHNEARSVLLRTLVSILIRSPLSLVKEIILVDDFSDNASDGLELDSLPKVKVIRNEKREGAVRSRIRGSDVAKGDVLTFLDSHCEVTDGWLEPLLATIKQNPTSIVSPVIDEI 222          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: A0A2C9JJX5_BIOGL (Glyco_trans_2-like domain-containing protein n=1 Tax=Biomphalaria glabrata TaxID=6526 RepID=A0A2C9JJX5_BIOGL)

HSP 1 Score: 108 bits (270), Expect = 3.910e-23
Identity = 57/124 (45.97%), Postives = 85/124 (68.55%), Query Frame = 2
Query:  497 ASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDGNA------AVEGSPVWSMPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVI 850
            AS+IM FYNE+ + L RS+ ++L++T  + L +IIL+DD ++      A+E     +  KV+L+R+K+R G++RARM G++YA GEVLVF D+HIE    WLEPL+  I  N +T+ VP +D I
Sbjct:  127 ASIIMCFYNEAWSTLLRSIHSILKQTPENLLKEIILIDDNSSLEHLGKALEDYITQNFSKVKLIRSKERLGLIRARMEGAKYATGEVLVFLDSHIEAGNTWLEPLLDRIAANRKTVVVPVVDTI 250          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: UPI001EE51F45 (polypeptide N-acetylgalactosaminyltransferase 5-like n=1 Tax=Haliotis rubra TaxID=36100 RepID=UPI001EE51F45)

HSP 1 Score: 109 bits (272), Expect = 4.340e-23
Identity = 64/157 (40.76%), Postives = 95/157 (60.51%), Query Frame = 2
Query:  425 RAVGKKQPPVCNLHYAPRTSTPQTASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDG-NAAVEGSPV----WSMPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDSVKTHSG 880
            RAV   + P C   +        + SV++ FYNE+ + L R++ ++L RT  + L +I+LVDDG N    G P+     +MPKV ++RT QR G++RARM G ++AR  VLVF D+H E   GWLEPL+  I++N  T+  PT+DVI  D  + ++ 
Sbjct:  174 RAVPDFRNPECQAAFQFYPKDLPSVSVVICFYNEAWSTLLRTIHSVLNRTPENLLEEILLVDDGSNLGDLGEPLDEYTAAMPKVHVIRTNQRLGLVRARMEGIKFARAPVLVFLDSHCECGYGWLEPLLVPIKDNPSTVVTPTIDVIDKDDFRINNA 330          
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Match: UPI0010BD8749 (polypeptide N-acetylgalactosaminyltransferase 11 n=1 Tax=Galendromus occidentalis TaxID=34638 RepID=UPI0010BD8749)

HSP 1 Score: 108 bits (271), Expect = 5.240e-23
Identity = 58/126 (46.03%), Postives = 86/126 (68.25%), Query Frame = 2
Query:  497 ASVIMSFYNESAANLERSVMTLLERTNWDALLDIILVDDGNAAVEGSP----VWSMPKVRLVRTKQREGIMRARMIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMTDS 862
            ASVI+ FYNE+ + L R+V ++L+R+    L +IILVDD +   E  P    V    KVR++RT++REG++RARMIG+  + G+VLVF D+H+EV E WL+PL+  I+ N  T+  P +D+I  D+
Sbjct:  132 ASVIICFYNEAWSTLIRTVNSVLDRSPSALLQEIILVDDLSDIAELEPLAGFVQKHEKVRVIRTREREGLIRARMIGAHNSTGDVLVFLDSHVEVNERWLQPLLVPIQQNQTTVTCPVIDIINADT 257          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig7376.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7J7K981_BUGNE8.250e-2546.09Glyco_trans_2-like domain-containing protein n=1 T... [more]
A0A6P7VCC4_IXOSC1.890e-2448.46Polypeptide N-acetylgalactosaminyltransferase n=4 ... [more]
B7PEU0_IXOSC2.090e-2448.46Polypeptide N-acetylgalactosaminyltransferase n=1 ... [more]
A0A433SW88_ELYCH8.320e-2444.19Polypeptide N-acetylgalactosaminyltransferase n=1 ... [more]
A0A2D0S4X9_ICTPU1.120e-2344.70Polypeptide N-acetylgalactosaminyltransferase n=7 ... [more]
UPI0008F9DE1D1.710e-2343.79polypeptide N-acetylgalactosaminyltransferase 35A-... [more]
A0A2T7NXH9_POMCA1.740e-2340.69Polypeptide N-acetylgalactosaminyltransferase n=3 ... [more]
A0A2C9JJX5_BIOGL3.910e-2345.97Glyco_trans_2-like domain-containing protein n=1 T... [more]
UPI001EE51F454.340e-2340.76polypeptide N-acetylgalactosaminyltransferase 5-li... [more]
UPI0010BD87495.240e-2346.03polypeptide N-acetylgalactosaminyltransferase 11 n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig7376contigC-tenellus_contig7376:60..1399 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog136037.KDR14208
PFAMsGlyco_transf_7C,Glycos_transf_2,Ricin_B_lectin
Model size881
Max annot lvl33208|Metazoa
KEGG koko:K00710
KEGG ReactionR05907
KEGG Pathwayko00512,ko01100,map00512,map01100
KEGG ModuleM00056
Hectar predicted targeting categorysignal peptide
GOsGO:0000003,GO:0003674,GO:0003824,GO:0004653,GO:0005975,GO:0008150,GO:0008152,GO:0008194,GO:0008376,GO:0009058,GO:0009311,GO:0009312,GO:0016051,GO:0016740,GO:0016757,GO:0016758,GO:0032501,GO:0032504,GO:0044238,GO:0071704,GO:0140096,GO:1901576
Exons3
Evalue9.04e-25
EggNOG OGsKOG3736@1|root,KOG3738@2759|Eukaryota,3AIIR@33154|Opisthokonta,3BY84@33208|Metazoa,3DDUW@33213|Bilateria,42271@6656|Arthropoda,3SQV7@50557|Insecta
EC2.4.1.41
DescriptionGlycosyl transferase family 2
Cds size774
COG categoryO
CAZyGT27
BRITEko00000,ko00001,ko00002,ko01000,ko01003
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790032.5757754-CDS-C-tenellus_contig7376:59..2341680790032.5757754-CDS-C-tenellus_contig7376:59..234Choristocarpus tenellus KU2346CDSC-tenellus_contig7376 60..234 -
1680790032.5871322-CDS-C-tenellus_contig7376:491..6401680790032.5871322-CDS-C-tenellus_contig7376:491..640Choristocarpus tenellus KU2346CDSC-tenellus_contig7376 492..640 -
1680790032.6001756-CDS-C-tenellus_contig7376:843..12931680790032.6001756-CDS-C-tenellus_contig7376:843..1293Choristocarpus tenellus KU2346CDSC-tenellus_contig7376 844..1293 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790032.6091478-UTR-C-tenellus_contig7376:1293..13991680790032.6091478-UTR-C-tenellus_contig7376:1293..1399Choristocarpus tenellus KU2346UTRC-tenellus_contig7376 1294..1399 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig7376.1.1prot_C-tenellus_contig7376.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig7376 60..1293 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig7376.1.1

>prot_C-tenellus_contig7376.1.1 ID=prot_C-tenellus_contig7376.1.1|Name=mRNA_C-tenellus_contig7376.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=258bp
MDIKRPLGCLGVLLAYAVTMFYLETIVERLAHHRGPRVDVAFFNQHSTGG
VSSPSRGHNWDSSCISGTFQCPCIELTSGVGCLDNLVCDVQNSLCELDPL
WRNFSTRAVGKKQPPVCNLHYAPRTSTPQTASVIMSFYNESAANLERSVM
TLLERTNWDALLDIILVDDGNAAVEGSPVWSMPKVRLVRTKQREGIMRAR
MIGSRYARGEVLVFFDAHIEVQEGWLEPLVGTIRNNYRTIAVPTLDVIMT
DSVKTHSG
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mRNA from alignment at C-tenellus_contig7376:60..1399-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig7376.1.1 ID=mRNA_C-tenellus_contig7376.1.1|Name=mRNA_C-tenellus_contig7376.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=1340bp|location=Sequence derived from alignment at C-tenellus_contig7376:60..1399- (Choristocarpus tenellus KU2346)
CTGTGGAAGGGGTCAAATGGAAGGTACACCACAGTTTTTCCGCAGTGAGA TGAGGTTAACTTGTCATTGTACTCTGTTCTTCTTTCAGTAGTATTAAGTA GTAAAGATGGACATAAAGCGTCCTCTGGGGTGCCTAGGAGTACTGCTCGC TTACGCAGTGACAATGTTTTATCTCGAGACAATCGTGGAACGTCTAGCAC ATCACCGTGGTCCGAGGGTAGATGTTGCGTTTTTCAACCAGCACAGCACT GGTGGTGTATCCTCACCTTCTCGAGGTCACAATTGGGACAGTTCTTGCAT TTCAGGAACATTCCAATGTCCCTGCATCGAACTTACGAGTGGGGTAGGGT GCTTGGATAACCTGGTCTGTGATGTTCAAAATAGTCTATGTGAGCTTGAC CCTCTATGGCGAAACTTCAGCACGCGAGCGGTTGGTAAGAAACAGCCTCC AGTCTGTAATCTACACTACGCGCCAAGAACCTCGACCCCGCAGACAGCTT CGGTGATCATGTCGTTCTACAATGAAAGCGCGGCAAATCTTGAGCGTTCG GTGATGGTGAGTACTTCACCAATGATATACTGGGGCAGTCCTTCACAGTC AGTTTGTTTGTGTTGATGTTGCACTTCTTCTACAAAGACATGCACTCTCG GCATACCTCTCTTTGCTGGAACTCTCTTCTCCAGTAGAAGATTGTAATTC GCTTGTAACCCAATTATGAACATACCCTTCCATTGCCATATTATGATCGT GGAGAGAAGACCCTGTTAGAGCGAACGAATTGGGATGCGCTATTGGACAT CATTCTGGTTGATGACGGAAACGCTGCTGTAGAAGGGTCCCCGGTTTGGT CCATGCCCAAGGTCAGACTGGTGCGCACAAAGCAACGCGAGGGGATCATG CGTGCTAGGTGAGACATGAGCGTGAAAGTTCAACTACTGATGATGCGGTT CTTCTCTCCCTCGATCATAGAAAATGTTTCCAGGTTGTTGACTGGATTGA CTATGTAGAGAAACAATTCATCTCCGAAGACTGCAATGGTTACCGTGTTG ATGCGTAGTGAAATAAGAACTACTTCCAATCTGCCCATTACTGTTTTGTT ATGTTTTGTCGATATAGCATACACCAATTACTTCCAACCACCTCTCTTCT GCCCTCTTTATGTAGGATGATTGGGTCGCGATACGCAAGAGGAGAAGTCC TAGTGTTCTTCGATGCTCACATTGAAGTCCAGGAAGGATGGTTGGAACCC TTAGTGGGCACTATCAGGAACAACTATCGCACCATTGCTGTGCCCACGTT GGATGTCATTATGACTGACAGTGTTAAGACCCACTCTGGG
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Coding sequence (CDS) from alignment at C-tenellus_contig7376:60..1399-

>mRNA_C-tenellus_contig7376.1.1 ID=mRNA_C-tenellus_contig7376.1.1|Name=mRNA_C-tenellus_contig7376.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=774bp|location=Sequence derived from alignment at C-tenellus_contig7376:60..1399- (Choristocarpus tenellus KU2346)
ATGGACATAAAGCGTCCTCTGGGGTGCCTAGGAGTACTGCTCGCTTACGC
AGTGACAATGTTTTATCTCGAGACAATCGTGGAACGTCTAGCACATCACC
GTGGTCCGAGGGTAGATGTTGCGTTTTTCAACCAGCACAGCACTGGTGGT
GTATCCTCACCTTCTCGAGGTCACAATTGGGACAGTTCTTGCATTTCAGG
AACATTCCAATGTCCCTGCATCGAACTTACGAGTGGGGTAGGGTGCTTGG
ATAACCTGGTCTGTGATGTTCAAAATAGTCTATGTGAGCTTGACCCTCTA
TGGCGAAACTTCAGCACGCGAGCGGTTGGTAAGAAACAGCCTCCAGTCTG
TAATCTACACTACGCGCCAAGAACCTCGACCCCGCAGACAGCTTCGGTGA
TCATGTCGTTCTACAATGAAAGCGCGGCAAATCTTGAGCGTTCGGTGATG
ACCCTGTTAGAGCGAACGAATTGGGATGCGCTATTGGACATCATTCTGGT
TGATGACGGAAACGCTGCTGTAGAAGGGTCCCCGGTTTGGTCCATGCCCA
AGGTCAGACTGGTGCGCACAAAGCAACGCGAGGGGATCATGCGTGCTAGG
ATGATTGGGTCGCGATACGCAAGAGGAGAAGTCCTAGTGTTCTTCGATGC
TCACATTGAAGTCCAGGAAGGATGGTTGGAACCCTTAGTGGGCACTATCA
GGAACAACTATCGCACCATTGCTGTGCCCACGTTGGATGTCATTATGACT
GACAGTGTTAAGACCCACTCTGGG
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