mRNA_C-tenellus_contig9344.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig9344.1.1
Unique NamemRNA_C-tenellus_contig9344.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A7S0Q1V6_9EUKA (Hypothetical protein n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0Q1V6_9EUKA)

HSP 1 Score: 122 bits (305), Expect = 2.770e-29
Identity = 66/118 (55.93%), Postives = 82/118 (69.49%), Query Frame = 1
Query:  883 GSISSKGNTMATAGVGNEQDFVKELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGAL 1236
            GS SSK + MA    G+    V++LV  + +MVFSK+YCPYCK AK  L+K GA F  +ELD   +G  IQ+ LL +TGQ+TVPNVFVGGKSIGGG+ET+ L +SGEL     AA AL
Sbjct:    2 GSSSSKVDAMAAQDAGS---LVEKLVEEHAVMVFSKSYCPYCKLAKEVLTKEGAKFHAIELDKRDDGNAIQNALLKLTGQKTVPNVFVGGKSIGGGSETKALSQSGELKKKLVAAKAL 116          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: D8LC00_ECTSI (Glutaredoxin n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LC00_ECTSI)

HSP 1 Score: 117 bits (293), Expect = 8.770e-28
Identity = 54/98 (55.10%), Postives = 74/98 (75.51%), Query Frame = 1
Query:  940 DFVKELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGA 1233
            D V E+V  + ++V+SKTYCP+C KAK AL  +GA ++++ELD   NG+ IQD L ++TGQR+VPNVF+GG SIGGG +T +LQ+SGEL+    A GA
Sbjct:    6 DAVNEMVGQHGVVVYSKTYCPFCTKAKKALKDVGAKYELIELDEVDNGSAIQDALQSITGQRSVPNVFIGGTSIGGGDDTVRLQKSGELLTKVTAVGA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A1R3GM95_COCAP (Glutaredoxin n=2 Tax=Corchorus TaxID=93758 RepID=A0A1R3GM95_COCAP)

HSP 1 Score: 117 bits (293), Expect = 9.590e-28
Identity = 56/97 (57.73%), Postives = 71/97 (73.20%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KE+V+ANP++VFSK+YCPYC   K  L K+ ASFK +ELDNES+G+ IQ  L   TGQRTVPNVF+GGK IGG   T  L + G+L+ +   AGA+A
Sbjct:    7 KEIVSANPVVVFSKSYCPYCVSVKQLLQKLDASFKAIELDNESDGSEIQSALAEWTGQRTVPNVFIGGKHIGGCDTTTALHQEGKLIPLLNQAGAIA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A076KWF9_TOBAC (Gluaredoxin n=3 Tax=Solanaceae TaxID=4070 RepID=A0A076KWF9_TOBAC)

HSP 1 Score: 117 bits (293), Expect = 9.880e-28
Identity = 58/97 (59.79%), Postives = 71/97 (73.20%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KE+V+ANP++VFSKTYCP+C   K+ LSK+GASF  +ELD E +G+ IQ  L   TGQRTVPNVF+GGK IGG   T  L R G+LV +   AGALA
Sbjct:    7 KEIVSANPVVVFSKTYCPFCVSVKDLLSKLGASFNAVELDTEKDGSEIQAALAEWTGQRTVPNVFIGGKHIGGCDATTALHREGKLVPLLTEAGALA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A7S1B3V4_9STRA (Hypothetical protein n=2 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1B3V4_9STRA)

HSP 1 Score: 117 bits (293), Expect = 1.050e-27
Identity = 55/110 (50.00%), Postives = 84/110 (76.36%), Query Frame = 1
Query:  910 MATAGVGNEQDFVKELVAANPIMVFSKTYCPYCKKAKNALSKIGAS-FKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGAL 1236
            M ++   +   ++++ + +N ++VFSKTYCP+C KAK +L+ +G + FK +ELD  ++G++IQ  LL++TGQRTVPNVF+GGKSIGGG +T  L++SG+L  M +AAGAL
Sbjct:    1 MGSSSSKDMSSYIQDTIGSNKVVVFSKTYCPFCTKAKRSLANVGCTNFKSVELDQMADGSSIQSGLLSLTGQRTVPNVFIGGKSIGGGDDTAALEQSGKLKTMLQAAGAL 110          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A6P5ZGL0_DURZI (glutaredoxin-like n=1 Tax=Durio zibethinus TaxID=66656 RepID=A0A6P5ZGL0_DURZI)

HSP 1 Score: 116 bits (290), Expect = 2.480e-27
Identity = 55/97 (56.70%), Postives = 72/97 (74.23%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KE+V+ANP++VFSKT+CP+C K K  L ++GASFK +ELDNES+G  IQ  L   TGQRTVPNVF+GGK IGG   T  + + G+L+ +   AGA+A
Sbjct:    7 KEIVSANPVVVFSKTHCPFCVKVKQLLQQLGASFKAIELDNESDGVEIQAALAQWTGQRTVPNVFIGGKHIGGCDATTAMHKDGKLIPLLTEAGAVA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A087GTB4_ARAAL (Glutaredoxin domain-containing protein n=2 Tax=Arabis TaxID=50451 RepID=A0A087GTB4_ARAAL)

HSP 1 Score: 116 bits (290), Expect = 2.790e-27
Identity = 56/97 (57.73%), Postives = 72/97 (74.23%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KELV++N ++VFSK+YCPYC + K  L ++GA+FK +ELDNES+GA +Q  L   TGQRTVPNVF+GGK IGG   T  L R G+LV +   AGA+A
Sbjct:    7 KELVSSNSVVVFSKSYCPYCVRVKELLQQLGATFKAIELDNESDGAAVQSALGEWTGQRTVPNVFIGGKHIGGCDATTNLHRDGKLVPLLTEAGAVA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A6P3ZZT2_ZIZJJ (glutaredoxin-like n=2 Tax=Ziziphus jujuba TaxID=326968 RepID=A0A6P3ZZT2_ZIZJJ)

HSP 1 Score: 115 bits (289), Expect = 3.410e-27
Identity = 53/97 (54.64%), Postives = 73/97 (75.26%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KE+V++NP++VFSKTYCPYC   K   +++GA+FKV+ELD ES+G+ IQ  L  +TGQRTVPNVF+GG  IGG   T+ L + G+L+ +   AGA+A
Sbjct:    7 KEIVSSNPVVVFSKTYCPYCVTVKQLFTQLGATFKVIELDTESDGSEIQSALAEITGQRTVPNVFIGGNHIGGCDNTKALHKDGKLITLLTEAGAVA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: A0A6P5YNJ8_DURZI (glutaredoxin-like n=1 Tax=Durio zibethinus TaxID=66656 RepID=A0A6P5YNJ8_DURZI)

HSP 1 Score: 115 bits (289), Expect = 3.410e-27
Identity = 56/97 (57.73%), Postives = 70/97 (72.16%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KE V+ANP++VFSK+YCPYC   K  L +IGASFK +ELDNES+GA IQ  L   TGQRTVPNVF+GG  IGG   T  + + G+L+ +   AGA+A
Sbjct:    7 KETVSANPVVVFSKSYCPYCVSVKKLLQQIGASFKAIELDNESDGAEIQAALAEWTGQRTVPNVFIGGNHIGGCDTTNAIHKDGKLIPLLTEAGAVA 103          
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Match: UPI000CED17C3 (glutaredoxin n=1 Tax=Morus notabilis TaxID=981085 RepID=UPI000CED17C3)

HSP 1 Score: 115 bits (289), Expect = 3.410e-27
Identity = 57/97 (58.76%), Postives = 71/97 (73.20%), Query Frame = 1
Query:  949 KELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVLELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELVNMARAAGALA 1239
            KE+V++NP+ VFSKTYCP+C   K  LS++GA+FK +ELD ES+GA IQ  L   TGQRTVPNVF+GGK IGG   T  L + G+LV +   AGALA
Sbjct:    7 KEIVSSNPVAVFSKTYCPFCASVKKLLSELGATFKAVELDTESDGAEIQAALAEWTGQRTVPNVFIGGKHIGGCDATMALHKEGKLVPLLTEAGALA 103          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig9344.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S0Q1V6_9EUKA2.770e-2955.93Hypothetical protein n=1 Tax=Coccolithus braarudii... [more]
D8LC00_ECTSI8.770e-2855.10Glutaredoxin n=2 Tax=Ectocarpus TaxID=2879 RepID=D... [more]
A0A1R3GM95_COCAP9.590e-2857.73Glutaredoxin n=2 Tax=Corchorus TaxID=93758 RepID=A... [more]
A0A076KWF9_TOBAC9.880e-2859.79Gluaredoxin n=3 Tax=Solanaceae TaxID=4070 RepID=A0... [more]
A0A7S1B3V4_9STRA1.050e-2750.00Hypothetical protein n=2 Tax=Corethron hystrix Tax... [more]
A0A6P5ZGL0_DURZI2.480e-2756.70glutaredoxin-like n=1 Tax=Durio zibethinus TaxID=6... [more]
A0A087GTB4_ARAAL2.790e-2757.73Glutaredoxin domain-containing protein n=2 Tax=Ara... [more]
A0A6P3ZZT2_ZIZJJ3.410e-2754.64glutaredoxin-like n=2 Tax=Ziziphus jujuba TaxID=32... [more]
A0A6P5YNJ8_DURZI3.410e-2757.73glutaredoxin-like n=1 Tax=Durio zibethinus TaxID=6... [more]
UPI000CED17C33.410e-2758.76glutaredoxin n=1 Tax=Morus notabilis TaxID=981085 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig9344contigC-tenellus_contig9344:3126..5966 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog4096.XP_009768204.1
PFAMsGlutaredoxin
Model size1802
Max annot lvl35493|Streptophyta
KEGG koko:K03676
Hectar predicted targeting categoryother localisation
Exons5
Evalue2.24e-33
EggNOG OGsCOG0695@1|root,KOG1752@2759|Eukaryota,37V77@33090|Viridiplantae,3GJCN@35493|Streptophyta,44KGC@71274|asterids
Ec32 ortholog descriptionGlutaredoxin
Ec32 orthologEc-20_003920.1
DescriptionGlutaredoxin
Cds size333
COG categoryO
BRITEko00000,ko03110
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig9344.1.1prot_C-tenellus_contig9344.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig9344 3686..4914 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790171.1448529-UTR-C-tenellus_contig9344:3125..36851680790171.1448529-UTR-C-tenellus_contig9344:3125..3685Choristocarpus tenellus KU2346UTRC-tenellus_contig9344 3126..3685 -
1680790171.2132246-UTR-C-tenellus_contig9344:4914..50021680790171.2132246-UTR-C-tenellus_contig9344:4914..5002Choristocarpus tenellus KU2346UTRC-tenellus_contig9344 4915..5002 -
1680790171.2232497-UTR-C-tenellus_contig9344:5145..59661680790171.2232497-UTR-C-tenellus_contig9344:5145..5966Choristocarpus tenellus KU2346UTRC-tenellus_contig9344 5146..5966 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790171.1597855-CDS-C-tenellus_contig9344:3685..37721680790171.1597855-CDS-C-tenellus_contig9344:3685..3772Choristocarpus tenellus KU2346CDSC-tenellus_contig9344 3686..3772 -
1680790171.1790087-CDS-C-tenellus_contig9344:4124..42261680790171.1790087-CDS-C-tenellus_contig9344:4124..4226Choristocarpus tenellus KU2346CDSC-tenellus_contig9344 4125..4226 -
1680790171.1898232-CDS-C-tenellus_contig9344:4439..45511680790171.1898232-CDS-C-tenellus_contig9344:4439..4551Choristocarpus tenellus KU2346CDSC-tenellus_contig9344 4440..4551 -
1680790171.201246-CDS-C-tenellus_contig9344:4882..49141680790171.201246-CDS-C-tenellus_contig9344:4882..4914Choristocarpus tenellus KU2346CDSC-tenellus_contig9344 4883..4914 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig9344.1.1

>prot_C-tenellus_contig9344.1.1 ID=prot_C-tenellus_contig9344.1.1|Name=mRNA_C-tenellus_contig9344.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=111bp
MATAGVGNEQDFVKELVAANPIMVFSKTYCPYCKKAKNALSKIGASFKVL
ELDNESNGATIQDTLLAMTGQRTVPNVFVGGKSIGGGTETEQLQRSGELV
NMARAAGALA*
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mRNA from alignment at C-tenellus_contig9344:3126..5966-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig9344.1.1 ID=mRNA_C-tenellus_contig9344.1.1|Name=mRNA_C-tenellus_contig9344.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=2841bp|location=Sequence derived from alignment at C-tenellus_contig9344:3126..5966- (Choristocarpus tenellus KU2346)
AAAAGGGTGAAAAATGGACGCCGACACCCCCCAGATACTTTGACCCAGGA CTCTACCACACCAAGTATCACCCTATAATGAGATCTCACACCTTTGAATT TTGTCACGAATGCCCCAGTAATACCAATGGTGGTGGCGAAAGGAAGGCAT GGTCTATGACTGATCGATGTTAGACCCGATATCCCCCCAGTTACTTATTT CACAGTAATCTAACCAACGATTGCCCTACAACTGGCCTCAACTGTAAAAT TAGTTCCAACTACAAATTGATGCATCTATCATTTTGTGCCAGCCCTAGCA TCGCGCTCAGTCCTCAACTATCTCTTTCACTATGGATCACACGAGATAAC ACCCTACAACATTTCTGACTCTTTTCATTTCATCTCTAATGCCCATTATT TGTCGCAAGATTCCACCCTACACTAAAATGCCCCAGAAGGTCTATTTCAC CACAAAAAATGCCTGGGAAACTTGCTCATACCATTTTTTCTGCTTGCAGC ACGCGTCTCCACCTTTCCTGGCGGACACTCCCACCCATTTCAATTGGGGG AAAGATTGGTCACAAGTTTGAGATACTCTTCCCTACAACCTCACCTCACC AGTTTTTTAACTTCTCGATTACTGATTCTACTTAAAAAAAATTCACTCTT CTACAACCACCATCCACCCTACCCACCTTTTTCTTAGTCAGAATGTAATG TGGTTTCTTTGTCTACCACAACCAATTCTGATGTACACAAAACTCGAATC ACTTCATACTCACTGATACAATAACCTGACATAATAACCCGATATAATAA CCCGATATTTTCTACGCAATTGTCCTTAATAAAAAAAAAGTGTATTATTG TAGGTTTTTGTAAAGGAATTATCATTTTAGTTATTTGATGGATCGAACCA CCCTCCAAAGCAAAACTCCTTCCACATAGACATACACATATCACAACCCC CCAACACACGTAAGAACCGCTGAAACAGAGCGTTGTAAGACGGTCCAGGT GGCTGAATAAAAGGAAGTAATCACGGGTTCTATTTCGAGTAAGGGAAACA CGATGGCCACAGCAGGGGTGGGCAACGAGCAGGAGTGAGTGTCGTGCTTG ATTTTTACGTTATCCACCGCATTGCCTGTTCAAAGTGTGACGCATTACCT CGCAACTCTCCCCAATAGTTAACTGAAAGAAATGTACCCCTCGTCGGATA TTGCGATGCTTTGTCCCACCATGCATTAAGTGACTTGAGTTCCTTCTCCC TGTTTGTATGGGTTGTCCCATGTCTACGTGAAACACAACTAGTAGGTTAG GTCCATCCCATATTTTTGAGGTATGGTCATCGACATACTCATTTTTCCTT CCAGTGCTTCCTGTTTCATGCATAAACCTCGTCTTATCCATCCGATGTTG ACCCCGAGTTTGCAGTTTCGTGAAGGAGTTGGTGGCGGCTAATCCTATAA TGGTGTTCTCCAAGACCTATTGTCCCTACTGCAAGAAGGCTAAGAATGCC TTAAGCAAAATTGGGGCTTCCTTTAAGGTACATATAGGAAGGAGAAAATA TAGACCATGAGACCAAAGTCTGGTACCAGGGGAAGCATGTTCAGTCCAGA TAGAAGACAGAATCGGGGGACAAGTGTATGTCATTAATCTCTCTGAAATA TTCGTCGGTGCATATCTTGAACATCGGCTGGGCTCTAGCACTTCCCCTGA ACCATCGGTCTAAAAGCGACATGGACTATTGATGATACAGGTGCTGGAGC TTGACAATGAGAGCAACGGTGCCACTATCCAGGATACACTACTTGCAATG ACTGGACAACGCACTGTTCCAAACGTATTTGTTGGCGGAAAGGTAAATTA AATTACTGCCGCTGACATATGAATGTTTTGCAGACGTGTCCTCAACTGCT GAACTGAACATACGATCTTAGAGATATGGCTCTTGAGCGTTCACAGGAGT AACCCAGGCTATTTCCTGCTCGTGCTTTTCTTGAATGTTGAAAGCTGGGA GATAATGACCCTGCCTAGTAGCTAGGAGTTGCGCTCATGTGCATGAAAGA TATTTGTTGCGCCTTATATAGAGGTGGTCCTATTATATATTAGGCTGCCC TTATCTCTTGTTGCTTGTTTGCGTATATCTAACCCAAACACTAACCCTAA CCCTACAATACCACTGGTGAAACCCCCTTTTGCTGTTCATTCAGAGCATT GGGGGTGGCACTGAGACTGAACAGCTACAGCGCTCAGGGGAACTGGTAAA CATGGCGAGGGCAGCTGGTGCACTTGCTTGAGCAGTATTGTTGCGGTCTC TTTGTAGGCCTCTTCTGTATGTCTTGTTGGACTTAATACCCGTTTTTGTT AACATGTGGGTATGTCAAATGAATTGTTTGAAGTGTACACAGTATTTTCA GGATGGTGCATTAGATGCAGTGGTCCAGTGAAGACCAGAGAAGTTCTGGT TGCGATCTGTTTTGCCATTGACTTCGGTGTCTTTCAAACTATATTTGTTT GGTGGCTGCTCTCTTAGTTTTGTTGATTCAAAGGAAATATGGAGGTGCTT TCCCTACAAATTTTCTCAATAGGGTATTATGTCGCATTGTACATGCGGGC CCTAACTGTGTGTATCAATGTTAAAATTTACATCTCATTCACTCTCAGAC ACACACATAAATCATGACTCGCCAAAGATAGAGAGGAGCATTACGATCTC ACTTTCTTTCTTTTCTGGAACTTGATGCAGACCATGTGCACAATGTTAGG CGAAATTCGCCTCGTCCTCCTGATTTATATTTTCATGTAATGACTTTCTG CGTAATGCAGAATCGATCTTCTTTTGTTCACTTAAAACGCT
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Coding sequence (CDS) from alignment at C-tenellus_contig9344:3126..5966-

>mRNA_C-tenellus_contig9344.1.1 ID=mRNA_C-tenellus_contig9344.1.1|Name=mRNA_C-tenellus_contig9344.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=333bp|location=Sequence derived from alignment at C-tenellus_contig9344:3126..5966- (Choristocarpus tenellus KU2346)
ATGGCCACAGCAGGGGTGGGCAACGAGCAGGATTTCGTGAAGGAGTTGGT
GGCGGCTAATCCTATAATGGTGTTCTCCAAGACCTATTGTCCCTACTGCA
AGAAGGCTAAGAATGCCTTAAGCAAAATTGGGGCTTCCTTTAAGGTGCTG
GAGCTTGACAATGAGAGCAACGGTGCCACTATCCAGGATACACTACTTGC
AATGACTGGACAACGCACTGTTCCAAACGTATTTGTTGGCGGAAAGAGCA
TTGGGGGTGGCACTGAGACTGAACAGCTACAGCGCTCAGGGGAACTGGTA
AACATGGCGAGGGCAGCTGGTGCACTTGCTTGA
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