mRNA_C-tenellus_contig825.2.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig825.2.1
Unique NamemRNA_C-tenellus_contig825.2.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: D7FWX3_ECTSI (Axonemal 1-beta dynein heavy chain dynein heavy chain n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWX3_ECTSI)

HSP 1 Score: 196 bits (499), Expect = 8.900e-55
Identity = 100/148 (67.57%), Postives = 118/148 (79.73%), Query Frame = 1 
Query:  115 QSLLLCPHHLFCLF-RYIEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRRVTAEFNQVQTQMQ 555
            Q+ L    + + LF RY+EGLRHEFDE RR PPLRPNEP FAGSA+WAHSLAR VEE+W+HLKNC++MA+++ SDEAE +L L A KEA+AAY VFVTVI DFK NRYQAWVDHLN++IE  PL  RLDVPLLRR   +  +VQTQ Q
Sbjct:  642 QACLKKTAYTYMLFVRYVEGLRHEFDENRRRPPLRPNEPPFAGSAVWAHSLARGVEEAWSHLKNCRHMAKAMASDEAEAALCLGACKEAQAAYTVFVTVIGDFKANRYQAWVDHLNSVIEVMPLGVRLDVPLLRRADTD-PRVQTQQQ 788
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A835Z4V1_9STRA (Dynein heavy chain n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4V1_9STRA)

HSP 1 Score: 129 bits (324), Expect = 2.920e-31
Identity = 75/156 (48.08%), Postives = 92/156 (58.97%), Query Frame = 1 
Query:  160 YIEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAE-----MSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLN-TLIE-------------------------KDP--LPSRLDVPLLRRVTAE 528
            Y+ GLR EFDE+RR+PPL PNEP FAGSALW  SLA++ E SW  +KNCK+MAR+     A      ++  ++A  +AEAA+  F  +IADFK  RYQAWVDHL  TL++                         K P  L  RLDVPLLRR TAE
Sbjct:  485 YVGGLRREFDERRRAPPLGPNEPQFAGSALWGASLAKMAEASWELVKNCKHMARAAEGRLASNFNFNLNFIMTAAHDAEAAHTAFAAIIADFKAARYQAWVDHLTATLLDGGSGXXXXXXXXXXXXXXXXXXXDAKKPAALGGRLDVPLLRRATAE 640
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A7S1XL19_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XL19_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 3.380e-20
Identity = 50/104 (48.08%), Postives = 60/104 (57.69%), Query Frame = 1 
Query:  142 LFCLFR-YIEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVD 450
            +F LFR  +E  R EFD  +R PPLR NEP +AGSALWA SL  L EESW  L +     R    D+AE            +AYN F+ V+ DFK  RYQAWV+
Sbjct:   38 IFVLFRTQVEKRRFEFDNNKRDPPLRANEPQYAGSALWARSLGALEEESWTALHSSTLGFRGREFDDAE------------SAYNSFIAVLHDFKEFRYQAWVE 129
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A7S3JQB6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JQB6_9STRA)

HSP 1 Score: 92.8 bits (229), Expect = 6.970e-19
Identity = 52/129 (40.31%), Postives = 78/129 (60.47%), Query Frame = 1 
Query:  145 FCLF-RYIEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCK-YMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHL---NTLIEKDPLPSRLDVPLLRR 516
            + LF + +E LR  FDE+RR PPLR NEP FAG+ALWA SLAR+V ++W  L     Y+ +   +D+ E+            AY   + V+ D+K +RY+ WV  L   +++I +  L S+L++PL+RR
Sbjct:  210 YALFTKTVESLRQVFDEQRREPPLRANEPKFAGAALWASSLARVVRDAWVLLHEASSYLTKMREADDGEV------------AYVQLLAVLNDYKASRYKEWVQSLEAHSSMIGESNLLSKLEIPLMRR 326
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A7S4EBF2_9STRA (Hypothetical protein (Fragment) n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4EBF2_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 5.350e-17
Identity = 50/126 (39.68%), Postives = 73/126 (57.94%), Query Frame = 1 
Query:  142 LFCLFRY-IEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRR 516
            L+ LF + +E +R EFDE RR+PPLR +EP +AGSALWA +LA+ VE SW+ L+   Y             +  S  KEAE AY   + V+ ++    Y+ WV  +  L +   L ++LD PL++R
Sbjct:   14 LYLLFIHDVEEVRREFDENRRAPPLRNDEPRWAGSALWATALAQNVEHSWSLLQAATYF------------MPTSETKEAEIAYKALMGVVDEYMAGCYKNWVGSMGAL-DSSTLQAKLDKPLMKR 126
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A7S4EBE8_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4EBE8_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 1.040e-16
Identity = 50/126 (39.68%), Postives = 73/126 (57.94%), Query Frame = 1 
Query:  142 LFCLFRY-IEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRR 516
            L+ LF + +E +R EFDE RR+PPLR +EP +AGSALWA +LA+ VE SW+ L+   Y             +  S  KEAE AY   + V+ ++    Y+ WV  +  L +   L ++LD PL++R
Sbjct:  512 LYLLFIHDVEEVRREFDENRRAPPLRNDEPRWAGSALWATALAQNVEHSWSLLQAATYF------------MPTSETKEAEIAYKALMGVVDEYMAGCYKNWVGSMGAL-DSSTLQAKLDKPLMKR 624
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A8J2WYK5_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WYK5_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 1.170e-16
Identity = 50/126 (39.68%), Postives = 73/126 (57.94%), Query Frame = 1 
Query:  142 LFCLFRY-IEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRR 516
            L+ LF + +E +R EFDE RR+PPLR +EP +AGSALWA +LA+ VE SW+ L+   Y             +  S  KEAE AY   + V+ ++    Y+ WV  +  L +   L ++LD PL++R
Sbjct:  538 LYLLFIHDVEEVRREFDENRRAPPLRNDEPRWAGSALWATALAQNVEHSWSLLQAATYF------------MPTSETKEAEIAYKALMGVVDEYMAGCYKNWVGSMGAL-DSSTLQAKLDKPLMKR 650
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A6H5KP60_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KP60_9PHAE)

HSP 1 Score: 84.3 bits (207), Expect = 1.360e-15
Identity = 44/63 (69.84%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:  367 VKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRRVTAEFNQVQTQMQ 555
            V+EA+AAY VFVTVI DFK NRYQAWVDHLN++IE  PL +RLDVPLLRR   +   VQTQ Q
Sbjct:  121 VREAQAAYTVFVTVIGDFKANRYQAWVDHLNSVIEVMPLGARLDVPLLRRADTDPG-VQTQQQ 182
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A482RZ06_9ARCH (DHC_N1 domain-containing protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RZ06_9ARCH)

HSP 1 Score: 80.5 bits (197), Expect = 2.660e-14
Identity = 45/128 (35.16%), Postives = 73/128 (57.03%), Query Frame = 1 
Query:  136 HHLFCLFRYIEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRRV 519
            +H+F   + ++ +R EF+  R +PPLR +EP +AGSALWAHSL+ +++  + +L   +Y+         E   G     E++  +     VI DFK +RY  W++ LN   + + L  RL+ PLLRR+
Sbjct:  606 NHMFD--KEVQTIRSEFELARLNPPLRLHEPQYAGSALWAHSLSVIIKHDYDYLVRLQYVL-------TERDFG-----ESKEDFLSLAQVIQDFKQSRYNLWLEDLNNKAKDNGLQMRLEKPLLRRL 719
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BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Match: A0A2R5GWD4_9STRA (Dynein heavy chain 2, axonemal n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GWD4_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 4.010e-12
Identity = 44/127 (34.65%), Postives = 69/127 (54.33%), Query Frame = 1 
Query:  142 LFCLF-RYIEGLRHEFDEKRRSPPLRPNEPWFAGSALWAHSLARLVEESWAHLKNCKYMARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNTLIEKDPLPSRLDVPLLRRV 519
            +F LF R ++ +R EFD+ R SPPLR +EP FAG+ALWA  L    +  W+ L    +      + EAE         E +++Y+  V V+ D+    YQ W+  ++ L +   L  +L++PL+ RV
Sbjct:  668 VFQLFGRELDQIRLEFDQFRASPPLRADEPRFAGAALWARGLQERADRDWSLLHGASHFLAK--TREAE---------EVKSSYDTLVQVLEDYAMKMYQDWMSQMHGLDQSHML-QKLEIPLMSRV 782
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig825.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
D7FWX3_ECTSI8.900e-5567.57Axonemal 1-beta dynein heavy chain dynein heavy ch... [more]
A0A835Z4V1_9STRA2.920e-3148.08Dynein heavy chain n=1 Tax=Tribonema minus TaxID=3... [more]
A0A7S1XL19_9STRA3.380e-2048.08Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A7S3JQB6_9STRA6.970e-1940.31Hypothetical protein (Fragment) n=1 Tax=Aureoumbra... [more]
A0A7S4EBF2_9STRA5.350e-1739.68Hypothetical protein (Fragment) n=2 Tax=Pelagomona... [more]
A0A7S4EBE8_9STRA1.040e-1639.68Hypothetical protein (Fragment) n=1 Tax=Pelagomona... [more]
A0A8J2WYK5_9STRA1.170e-1639.68Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]
A0A6H5KP60_9PHAE1.360e-1569.84Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A482RZ06_9ARCH2.660e-1435.16DHC_N1 domain-containing protein (Fragment) n=1 Ta... [more]
A0A2R5GWD4_9STRA4.010e-1234.65Dynein heavy chain 2, axonemal n=1 Tax=Hondaea fer... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig825contigC-tenellus_contig825:4127..6074 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FWX3
Preferred nameDNAH2
PFAMsAAA_5,AAA_6,AAA_7,AAA_8,AAA_9,DHC_N1,DHC_N2,Dynein_heavy,MT
Model size557
Max annot lvl2759|Eukaryota
KEGG koko:K10408
KEGG Pathwayko05016,map05016
Hectar predicted targeting categoryother localisation
GOsGO:0000226,GO:0001539,GO:0001578,GO:0003341,GO:0003674,GO:0003774,GO:0003777,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005856,GO:0005858,GO:0005875,GO:0005929,GO:0005930,GO:0006928,GO:0006996,GO:0007010,GO:0007017,GO:0007018,GO:0008017,GO:0008092,GO:0008150,GO:0008569,GO:0009987,GO:0015630,GO:0015631,GO:0016043,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0022607,GO:0030030,GO:0030031,GO:0030286,GO:0031514,GO:0032838,GO:0032991,GO:0034622,GO:0035082,GO:0036156,GO:0036159,GO:0040011,GO:0042623,GO:0042995,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043933,GO:0044085,GO:0044422,GO:0044424,GO:0044430,GO:0044441,GO:0044444,GO:0044446,GO:0044447,GO:0044463,GO:0044464,GO:0044782,GO:0045503,GO:0045505,GO:0048870,GO:0051179,GO:0051674,GO:0051959,GO:0060271,GO:0060285,GO:0060294,GO:0065003,GO:0070286,GO:0070925,GO:0071840,GO:0097014,GO:0099568,GO:0120025,GO:0120031,GO:0120036,GO:0120038,GO:1902494,GO:1990939
Exons3
Evalue3.91e-23
EggNOG OGsCOG5245@1|root,KOG3595@2759|Eukaryota
Descriptiondynein light chain binding
Cds size243
COG categoryZ
BRITEko00000,ko00001,ko04812
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790090.3595388-UTR-C-tenellus_contig825:4126..44381680790090.3595388-UTR-C-tenellus_contig825:4126..4438Choristocarpus tenellus KU2346UTRC-tenellus_contig825 4127..4438 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790090.3835926-CDS-C-tenellus_contig825:4438..44461680790090.3835926-CDS-C-tenellus_contig825:4438..4446Choristocarpus tenellus KU2346CDSC-tenellus_contig825 4439..4446 +
1680790090.3943558-CDS-C-tenellus_contig825:5047..51581680790090.3943558-CDS-C-tenellus_contig825:5047..5158Choristocarpus tenellus KU2346CDSC-tenellus_contig825 5048..5158 +
1680790090.403246-CDS-C-tenellus_contig825:5950..60741680790090.403246-CDS-C-tenellus_contig825:5950..6074Choristocarpus tenellus KU2346CDSC-tenellus_contig825 5951..6074 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig825.2.1prot_C-tenellus_contig825.2.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig825 4439..6074 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig825.2.1

>prot_C-tenellus_contig825.2.1 ID=prot_C-tenellus_contig825.2.1|Name=mRNA_C-tenellus_contig825.2.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=81bp
MARSLVSDEAEMSLGLSAVKEAEAAYNVFVTVIADFKNNRYQAWVDHLNT
LIEKDPLPSRLDVPLLRRVTAEFNQVQTQMQ
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mRNA from alignment at C-tenellus_contig825:4127..6074+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig825.2.1 ID=mRNA_C-tenellus_contig825.2.1|Name=mRNA_C-tenellus_contig825.2.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=1948bp|location=Sequence derived from alignment at C-tenellus_contig825:4127..6074+ (Choristocarpus tenellus KU2346)
CCTTGTAACCTGCAGCTACCATTTGTGAATGTAATTTGACTCCGAATCTG
CTTGCTTTGCTTCCCAATCGTCCCCTCCTATTGACTTGGTTTGTGCCCCT
TGTCTCCTACAATACAGTCTCTTCTGCTCTGTCCACATCACCTCTTCTGC
CTGTTTAGATACATTGAGGGTCTTCGTCACGAATTTGATGAGAAGCGGCG
CAGTCCACCCCTCCGCCCCAATGAGCCATGGTTTGCAGGATCTGCATTGT
GGGCACATTCTCTCGCGCGCCTGGTTGAGGAGAGTTGGGCACACCTCAAG
AACTGCAAGTACATGGCAAGGTGAACTCAATCATAAGATGCTATGCATTT
TGAGGATTGGCATGCACCTGTCGAAGTGGAGTTTCTGGATGCAATGAGAT
GTCAGATCTGCTATGGCCTTTGTTAGGCAGAGAAAGTACGCCAGAGAGAG
AGCAGTGTTGTAAACTTTGCTTTGACCTTGGGGGCAGGATAGACTGAGAA
GCTTCAGTCTTTCAATCAACACACTGCATCAGAGGATTGAGTAAGGTCTG
CAAGAAGAGTCAAAGAGAGCACTTGCAGTCAGGAATCTCTAGTACAAAGA
CTTCTCTCTTGTTTTCATTATCCCATGATAAAGATTGCAGGTGTGAACAT
GCCTTCCCAATGTGGGGGAGGTACTCTTGAGGGCATCTGCTAGTGCAAGG
TGCTGCAGGGTGACTGCTTGGATTACTATTAGGACTCTGCCTTGCTCCAG
ACTGTAATGAAAATCGTGGGCTCTACCAACCATGCACCCAGAAAGTTACA
TTGTATGTTTTTTCCTCACTCCTGGAAGATTGTAAACCTAGGTAACTCAC
TTCCACAATTCTCCTTGCTAGCACGAATACCATTCTTCACTTCTCACATC
TTTTTGATTGATACTTATTAGGTCCCTTGTCTCTGATGAGGCTGAGATGA
GCCTAGGTCTTAGTGCTGTCAAGGAGGCGGAGGCTGCATACAATGTGTTT
GTCACTGTCATTGCTGACTTCAAGAACAACAGGTGGGAGATGTATGATGT
CTAGACTAGCCCTGGACAGTAAGCTGCTGTCGATCACTCTCCAAAGTACC
ATATTAGTTGGAGGAGAGCAAAAACGTTGATCAAAATATGTCAGATCATG
ATAGGAACATAATTCAACTTTAAATGGGTAGTTGTTCATGATTTCCACAT
TAGAACATCATTAGTAGCATTGACTGTTTTCAAGACGAATAGTGCTTTGA
AGCATAGATTTGAGTGTTGACCAGATAGCAATTATGGTAGTATGTACTAC
ATAGTAAAAAAAATAGAATTGAGAAAACTTGCTAGGGATAGCAGAACAAG
TATGTCCTACTAAAGAATGTAAAACTTAGGCTGTCCATTCTCTTGCCAAG
AAAAGCAGTAAGCTGATATTGCATGATCTACTATTCCCCAGGAAAAGCTG
GCTAGGGTTAGGGGGTACAGATGAATGTCGAAGAGGTTTTTTCGCTGTTG
ATGTTTTTTTTGGTTGGGACTATGTGGATGGCACCCTCGCAGTCCAACTG
CAGTTTCTTGTAGCCAGCTCTTGGTGGAAATGCCACAATTGTGAAGTTCT
TGCTCATCAAACTGTCTCAAAGATCGAATGGATGATAGAGTATACCAGAA
GATCCTTTTGTCGTCACTATACATGCTCTGTATATCGTGCTCTAGCAGCC
TACTTCTCCACCCCTACCTCATGATCACCCTGACTCTGTCCCAAAGTTTG
ATTATGATCAAGAAGCGAAGGTCCCCGATGGAATCTCACCTGTACACATG
ATGTGCATCCTGTGCCTTCTGCAGGTATCAGGCATGGGTGGATCATCTGA
ACACCCTTATCGAAAAGGATCCTCTCCCTAGCCGTCTGGATGTGCCTCTC
CTGCGGCGAGTCACGGCAGAGTTTAACCAAGTCCAAACTCAGATGCAA
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Coding sequence (CDS) from alignment at C-tenellus_contig825:4127..6074+

>mRNA_C-tenellus_contig825.2.1 ID=mRNA_C-tenellus_contig825.2.1|Name=mRNA_C-tenellus_contig825.2.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=243bp|location=Sequence derived from alignment at C-tenellus_contig825:4127..6074+ (Choristocarpus tenellus KU2346)
ATGGCAAGGTCCCTTGTCTCTGATGAGGCTGAGATGAGCCTAGGTCTTAG
TGCTGTCAAGGAGGCGGAGGCTGCATACAATGTGTTTGTCACTGTCATTG
CTGACTTCAAGAACAACAGGTATCAGGCATGGGTGGATCATCTGAACACC
CTTATCGAAAAGGATCCTCTCCCTAGCCGTCTGGATGTGCCTCTCCTGCG
GCGAGTCACGGCAGAGTTTAACCAAGTCCAAACTCAGATGCAA
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