mRNA_C-tenellus_contig76.5.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig76.5.1
Unique NamemRNA_C-tenellus_contig76.5.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: D7FWI9_ECTSI (Histone H2A n=6 Tax=Ectocarpus TaxID=2879 RepID=D7FWI9_ECTSI)

HSP 1 Score: 219 bits (557), Expect = 9.420e-72
Identity = 116/124 (93.55%), Postives = 121/124 (97.58%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAKK 372
            MSGKGKGGRG  KKSTTRSAKAGLQFPVGRVGRFLK+GKYA+RVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARI+PRHIQLAVRNDEELNKLLG+VTIASGGVLPNIHAVLLPKK+ KK
Sbjct:    1 MSGKGKGGRG--KKSTTRSAKAGLQFPVGRVGRFLKKGKYASRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIIPRHIQLAVRNDEELNKLLGEVTIASGGVLPNIHAVLLPKKTVKK 122
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: D7G193_ECTSI (Histone H2A n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G193_ECTSI)

HSP 1 Score: 217 bits (552), Expect = 3.230e-70
Identity = 115/123 (93.50%), Postives = 120/123 (97.56%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAK 369
            MSGKGKGGRG  KKSTTRSAKAGLQFPVGRVGRFLK+GKYA+RVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARI+PRHIQLAVRNDEELNKLLG+VTIASGGVLPNIHAVLLPKK+ K
Sbjct:   46 MSGKGKGGRG--KKSTTRSAKAGLQFPVGRVGRFLKKGKYASRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIIPRHIQLAVRNDEELNKLLGEVTIASGGVLPNIHAVLLPKKTVK 166
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: A0A6H5KG79_9PHAE (Histone H2A n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KG79_9PHAE)

HSP 1 Score: 210 bits (535), Expect = 3.260e-68
Identity = 110/122 (90.16%), Postives = 119/122 (97.54%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSA 366
            MSGKGKGGRG  KKST++S+KAGLQFPV R+GR+LK+GKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARI+PRHIQLAVRNDEELNKLLG+VTIASGGVLPNIHAVLLPKKS+
Sbjct:    1 MSGKGKGGRG--KKSTSKSSKAGLQFPVARIGRYLKRGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIIPRHIQLAVRNDEELNKLLGEVTIASGGVLPNIHAVLLPKKSS 120
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: A0A6U2JFS4_9STRA (Histone H2A n=6 Tax=Bacillariophyta TaxID=2836 RepID=A0A6U2JFS4_9STRA)

HSP 1 Score: 208 bits (530), Expect = 1.230e-67
Identity = 110/123 (89.43%), Postives = 117/123 (95.12%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAK 369
            MSGKGKGGRG  KKSTT SAKAGLQFPVGR+GR+L+QGKYATR+GAGAPVYLAAVLEYL AE+LELAGNAARDNKK+RIVPRHI LAV+NDEELNKLLG VTIASGGVLPNIHAVLLPKKSAK
Sbjct:    1 MSGKGKGGRGG-KKSTTSSAKAGLQFPVGRIGRYLRQGKYATRMGAGAPVYLAAVLEYLCAEILELAGNAARDNKKSRIVPRHITLAVKNDEELNKLLGGVTIASGGVLPNIHAVLLPKKSAK 122
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: D7FVQ5_ECTSI (Histone H2A n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVQ5_ECTSI)

HSP 1 Score: 210 bits (535), Expect = 1.680e-67
Identity = 110/122 (90.16%), Postives = 119/122 (97.54%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSA 366
            MSGKGKGGRG  KKST++S+KAGLQFPV R+GR+LK+GKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARI+PRHIQLAVRNDEELNKLLG+VTIASGGVLPNIHAVLLPKKS+
Sbjct:   52 MSGKGKGGRG--KKSTSKSSKAGLQFPVARIGRYLKRGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIIPRHIQLAVRNDEELNKLLGEVTIASGGVLPNIHAVLLPKKSS 171
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: A0A448ZH28_9STRA (Histone H2A n=12 Tax=Bacillariophyta TaxID=2836 RepID=A0A448ZH28_9STRA)

HSP 1 Score: 207 bits (528), Expect = 2.490e-67
Identity = 108/123 (87.80%), Postives = 117/123 (95.12%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAK 369
            MSGKGKGGRG+ KKSTT SAKAGLQFPVGR+GR+L+QGKYATR+GAGAPVYLAAVLEYL AE+LELAGNAARDNKK+RIVPRHI LAV+NDEELNKLLG VTIASGGVLPNIHA+LLPKKS K
Sbjct:    1 MSGKGKGGRGE-KKSTTASAKAGLQFPVGRIGRYLRQGKYATRMGAGAPVYLAAVLEYLCAEILELAGNAARDNKKSRIVPRHITLAVKNDEELNKLLGGVTIASGGVLPNIHAILLPKKSGK 122
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: D8LLP0_ECTSI (Histone H2A n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LLP0_ECTSI)

HSP 1 Score: 207 bits (526), Expect = 4.560e-67
Identity = 108/120 (90.00%), Postives = 115/120 (95.83%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKK 360
            MSGKGKGGRG  KK T+RSAKAGLQFPVGR+GRF+KQGKY++RVG GAPVY+AAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELN+LLGD TIASGGVLPNIHAVLLPKK
Sbjct:    1 MSGKGKGGRG--KKITSRSAKAGLQFPVGRIGRFIKQGKYSSRVGGGAPVYMAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNRLLGDATIASGGVLPNIHAVLLPKK 118
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: A0A1E7EZ55_9STRA (Histone H2A n=3 Tax=Bacillariophyta TaxID=2836 RepID=A0A1E7EZ55_9STRA)

HSP 1 Score: 205 bits (522), Expect = 3.120e-66
Identity = 107/127 (84.25%), Postives = 117/127 (92.13%), Query Frame = 1 
Query:    1 MSGKGK--GGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAKKA 375
            MSGKGK  GGRG+ K STT SAKAGLQFPVGR+GR+L+QGKYATR+GAGAPVYLAAVLEYL AE+LELAGNAARDNKK+RI+PRHI LAV+NDEELNKLLGDVTIA GGVLPNIHAVLLPKKS K +
Sbjct:    1 MSGKGKXXGGRGEKKVSTTSSAKAGLQFPVGRIGRYLRQGKYATRMGAGAPVYLAAVLEYLCAEILELAGNAARDNKKSRIIPRHITLAVKNDEELNKLLGDVTIAQGGVLPNIHAVLLPKKSQKSS 127
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: A0A6U3UCP9_9STRA (Histone H2A n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6U3UCP9_9STRA)

HSP 1 Score: 205 bits (522), Expect = 4.940e-66
Identity = 108/123 (87.80%), Postives = 117/123 (95.12%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAK 369
            MSGKGK GRG+ KKSTT SAKAGLQFPVGR+GR+L+QGKYATR+GAGAPVYLAAVLEYL AE+LELAGNAARDNKK+RIVPRHI LAV+NDEELNKLLG VTIASGGVLPNIHAVLLPKKS+K
Sbjct:   28 MSGKGKEGRGE-KKSTTASAKAGLQFPVGRIGRYLRQGKYATRMGAGAPVYLAAVLEYLCAEILELAGNAARDNKKSRIVPRHITLAVKNDEELNKLLGGVTIASGGVLPNIHAVLLPKKSSK 149
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BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Match: A0A061QUG5_9CHLO (Histone H2A n=4 Tax=core chlorophytes TaxID=2692248 RepID=A0A061QUG5_9CHLO)

HSP 1 Score: 204 bits (520), Expect = 6.100e-66
Identity = 107/124 (86.29%), Postives = 115/124 (92.74%), Query Frame = 1 
Query:    1 MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGDVTIASGGVLPNIHAVLLPKKSAKK 372
            MSG+GKG     KK+ +RSAKAGLQFPVGRV R+LK+GKYATR+GAGAPVYLAAVLEYLTAEVLELAGNAARDNKK RIVPRHIQLAVRNDEEL+KLLGDVTIASGGVLPNIH+VLLPKKS KK
Sbjct:    1 MSGRGKGKSAAGKKAVSRSAKAGLQFPVGRVARYLKKGKYATRIGAGAPVYLAAVLEYLTAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIASGGVLPNIHSVLLPKKSDKK 124
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig76.5.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FWI9_ECTSI9.420e-7293.55Histone H2A n=6 Tax=Ectocarpus TaxID=2879 RepID=D7... [more]
D7G193_ECTSI3.230e-7093.50Histone H2A n=1 Tax=Ectocarpus siliculosus TaxID=2... [more]
A0A6H5KG79_9PHAE3.260e-6890.16Histone H2A n=1 Tax=Ectocarpus sp. CCAP 1310/34 Ta... [more]
A0A6U2JFS4_9STRA1.230e-6789.43Histone H2A n=6 Tax=Bacillariophyta TaxID=2836 Rep... [more]
D7FVQ5_ECTSI1.680e-6790.16Histone H2A n=1 Tax=Ectocarpus siliculosus TaxID=2... [more]
A0A448ZH28_9STRA2.490e-6787.80Histone H2A n=12 Tax=Bacillariophyta TaxID=2836 Re... [more]
D8LLP0_ECTSI4.560e-6790.00Histone H2A n=2 Tax=Ectocarpus TaxID=2879 RepID=D8... [more]
A0A1E7EZ55_9STRA3.120e-6684.25Histone H2A n=3 Tax=Bacillariophyta TaxID=2836 Rep... [more]
A0A6U3UCP9_9STRA4.940e-6687.80Histone H2A n=1 Tax=Ditylum brightwellii TaxID=492... [more]
A0A061QUG5_9CHLO6.100e-6686.29Histone H2A n=4 Tax=core chlorophytes TaxID=269224... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig76contigC-tenellus_contig76:18515..18892 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FWI9
Preferred nameH2AFY
PFAMsHistone,Histone_H2A_C,Macro
Model size378
Max annot lvl2759|Eukaryota
KEGG koko:K11251,ko:K16617
KEGG Pathwayko04217,ko04974,ko05034,ko05322,map04217,map04974,map05034,map05322
Hectar predicted targeting categoryother localisation
GOsGO:0000122,GO:0000182,GO:0000228,GO:0000775,GO:0000785,GO:0000786,GO:0000790,GO:0000792,GO:0000793,GO:0000803,GO:0000805,GO:0000976,GO:0000977,GO:0000979,GO:0001012,GO:0001046,GO:0001047,GO:0001067,GO:0001739,GO:0001740,GO:0001932,GO:0001933,GO:0003674,GO:0003676,GO:0003677,GO:0003682,GO:0003690,GO:0004857,GO:0004860,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005720,GO:0005721,GO:0005730,GO:0005813,GO:0005815,GO:0005856,GO:0006323,GO:0006325,GO:0006333,GO:0006334,GO:0006342,GO:0006355,GO:0006356,GO:0006357,GO:0006469,GO:0006996,GO:0007063,GO:0007088,GO:0007275,GO:0007346,GO:0007399,GO:0007417,GO:0007420,GO:0007549,GO:0008104,GO:0008150,GO:0009889,GO:0009890,GO:0009892,GO:0009893,GO:0009987,GO:0010385,GO:0010468,GO:0010556,GO:0010558,GO:0010563,GO:0010564,GO:0010604,GO:0010605,GO:0010628,GO:0010629,GO:0010638,GO:0010639,GO:0010948,GO:0015630,GO:0016043,GO:0016458,GO:0016479,GO:0019207,GO:0019210,GO:0019216,GO:0019219,GO:0019220,GO:0019222,GO:0019887,GO:0019899,GO:0019900,GO:0019901,GO:0022607,GO:0030234,GO:0030291,GO:0030856,GO:0030858,GO:0031056,GO:0031057,GO:0031060,GO:0031061,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031399,GO:0031400,GO:0031490,GO:0031491,GO:0031492,GO:0031497,GO:0031974,GO:0031981,GO:0032268,GO:0032269,GO:0032501,GO:0032502,GO:0032879,GO:0032880,GO:0032991,GO:0032993,GO:0033036,GO:0033043,GO:0033044,GO:0033045,GO:0033047,GO:0033127,GO:0033128,GO:0033365,GO:0033673,GO:0034091,GO:0034093,GO:0034182,GO:0034184,GO:0034502,GO:0034613,GO:0034622,GO:0034728,GO:0040029,GO:0042325,GO:0042326,GO:0043086,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043549,GO:0043565,GO:0043933,GO:0044085,GO:0044092,GO:0044212,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044430,GO:0044446,GO:0044454,GO:0044464,GO:0044815,GO:0044877,GO:0045184,GO:0045595,GO:0045597,GO:0045604,GO:0045606,GO:0045616,GO:0045618,GO:0045682,GO:0045684,GO:0045786,GO:0045787,GO:0045814,GO:0045815,GO:0045840,GO:0045859,GO:0045876,GO:0045892,GO:0045931,GO:0045934,GO:0045936,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048731,GO:0048856,GO:0050789,GO:0050790,GO:0050793,GO:0050794,GO:0051094,GO:0051128,GO:0051129,GO:0051130,GO:0051171,GO:0051172,GO:0051174,GO:0051179,GO:0051234,GO:0051239,GO:0051240,GO:0051246,GO:0051248,GO:0051252,GO:0051253,GO:0051276,GO:0051338,GO:0051348,GO:0051569,GO:0051572,GO:0051641,GO:0051726,GO:0051783,GO:0051785,GO:0051983,GO:0051984,GO:0060255,GO:0060322,GO:0060341,GO:0061085,GO:0061086,GO:0062033,GO:0065003,GO:0065004,GO:0065007,GO:0065009,GO:0070013,GO:0070199,GO:0070727,GO:0071103,GO:0071168,GO:0071169,GO:0071824,GO:0071840,GO:0071900,GO:0071901,GO:0072594,GO:0080090,GO:0080134,GO:0090068,GO:0097159,GO:0098687,GO:0098772,GO:1901363,GO:1901836,GO:1901837,GO:1901987,GO:1901988,GO:1902275,GO:1902679,GO:1902749,GO:1902750,GO:1902882,GO:1902883,GO:1902884,GO:1903506,GO:1903507,GO:1903827,GO:1903828,GO:1904814,GO:1904815,GO:1905268,GO:1990837,GO:1990841,GO:2000026,GO:2000112,GO:2000113,GO:2001141,GO:2001251,GO:2001252
Exons1
Evalue1.54e-72
EggNOG OGsCOG5262@1|root,KOG1756@2759|Eukaryota
DescriptionHistone H2A
Cds size378
COG categoryB
BRITEko00000,ko00001,ko00536,ko03036,ko04147
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790046.3887937-CDS-C-tenellus_contig76:18514..188921680790046.3887937-CDS-C-tenellus_contig76:18514..18892Choristocarpus tenellus KU2346CDSC-tenellus_contig76 18515..18892 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig76.5.1prot_C-tenellus_contig76.5.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig76 18515..18892 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig76.5.1

>prot_C-tenellus_contig76.5.1 ID=prot_C-tenellus_contig76.5.1|Name=mRNA_C-tenellus_contig76.5.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=126bp
MSGKGKGGRGDSKKSTTRSAKAGLQFPVGRVGRFLKQGKYATRVGAGAPV
YLAAVLEYLTAEVLELAGNAARDNKKARIVPRHIQLAVRNDEELNKLLGD
VTIASGGVLPNIHAVLLPKKSAKKA*
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mRNA from alignment at C-tenellus_contig76:18515..18892+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig76.5.1 ID=mRNA_C-tenellus_contig76.5.1|Name=mRNA_C-tenellus_contig76.5.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=378bp|location=Sequence derived from alignment at C-tenellus_contig76:18515..18892+ (Choristocarpus tenellus KU2346)
ATGTCTGGCAAGGGAAAGGGTGGACGTGGTGACTCCAAGAAGTCGACCAC
ACGGTCAGCAAAGGCGGGTCTTCAGTTCCCTGTCGGTCGTGTTGGGCGTT
TCCTGAAGCAGGGCAAGTATGCCACCCGCGTTGGTGCTGGTGCTCCTGTA
TACCTCGCTGCAGTACTGGAGTACCTGACTGCTGAGGTGTTGGAGCTGGC
TGGGAATGCTGCACGTGACAACAAGAAGGCTCGCATTGTCCCTCGTCACA
TCCAGCTGGCGGTTCGCAACGACGAGGAGTTGAACAAGTTGCTTGGTGAC
GTGACCATCGCCTCTGGGGGTGTGTTACCCAATATCCACGCTGTTCTTTT
GCCTAAGAAGTCTGCAAAGAAGGCTTAA
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Coding sequence (CDS) from alignment at C-tenellus_contig76:18515..18892+

>mRNA_C-tenellus_contig76.5.1 ID=mRNA_C-tenellus_contig76.5.1|Name=mRNA_C-tenellus_contig76.5.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=378bp|location=Sequence derived from alignment at C-tenellus_contig76:18515..18892+ (Choristocarpus tenellus KU2346)
ATGTCTGGCAAGGGAAAGGGTGGACGTGGTGACTCCAAGAAGTCGACCAC
ACGGTCAGCAAAGGCGGGTCTTCAGTTCCCTGTCGGTCGTGTTGGGCGTT
TCCTGAAGCAGGGCAAGTATGCCACCCGCGTTGGTGCTGGTGCTCCTGTA
TACCTCGCTGCAGTACTGGAGTACCTGACTGCTGAGGTGTTGGAGCTGGC
TGGGAATGCTGCACGTGACAACAAGAAGGCTCGCATTGTCCCTCGTCACA
TCCAGCTGGCGGTTCGCAACGACGAGGAGTTGAACAAGTTGCTTGGTGAC
GTGACCATCGCCTCTGGGGGTGTGTTACCCAATATCCACGCTGTTCTTTT
GCCTAAGAAGTCTGCAAAGAAGGCTTAA
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