mRNA_C-tenellus_contig10925.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig10925.1.1
Unique NamemRNA_C-tenellus_contig10925.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: D8LC12_ECTSI (GBP5, guanylate-binding protein (Divergent) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LC12_ECTSI)

HSP 1 Score: 242 bits (618), Expect = 1.970e-71
Identity = 121/153 (79.08%), Postives = 136/153 (88.89%), Query Frame = 1
Query:   76 MAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGS-RVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDLG 531
            MAV + T + +L  LG +  VN+VAIFGAARGGKSFLMNQLAG+D +FKISNDKDPCTQGVDVSR V+PL +FA++ GCT+PRGVS S +VRVLFADVEGQGDRD AYDARLASAILP+ KCV+FNWRDSLQVDRMLNLLGVMQRAAHNVDLG
Sbjct:    1 MAVNKSTVDDILDKLGDEGAVNVVAIFGAARGGKSFLMNQLAGQDDIFKISNDKDPCTQGVDVSRAVVPLKDFATLDGCTAPRGVSTSNQVRVLFADVEGQGDRDAAYDARLASAILPLTKCVIFNWRDSLQVDRMLNLLGVMQRAAHNVDLG 153          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A7S3URQ4_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3URQ4_HETAK)

HSP 1 Score: 171 bits (432), Expect = 4.370e-51
Identity = 87/161 (54.04%), Postives = 114/161 (70.81%), Query Frame = 1
Query:   52 VAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSR-VRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDLG 531
            +A    PIM V Q  A+ VL PLG    +NL++IFGAAR GKSFLMN L+G++ +F+ISN++DPCTQG+D+S   + L +FAS+        +  S+ ++V F D EGQGDRD  YDARL S  L  ++CV+FNW+DSLQ DR+LNLLGVM RAA NV+ G
Sbjct:    1 MANSDSPIMEVDQKVADEVLTPLGPDTNLNLISIFGAARQGKSFLMNLLSGQENIFQISNNQDPCTQGIDISGNFVSLKDFASVDSPADGAAIDTSKGMQVGFVDAEGQGDRDAEYDARLVSPALLASRCVLFNWKDSLQRDRILNLLGVMSRAAMNVEKG 161          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A835YY61_9STRA (SAP domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YY61_9STRA)

HSP 1 Score: 181 bits (458), Expect = 1.850e-49
Identity = 101/174 (58.05%), Postives = 118/174 (67.82%), Query Frame = 1
Query:   19 TGAMKWITMADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDR------MLNLLGVMQRAAHNV 522
            TGA+KW+T+ D    P     V+ P AETVL+PLG K PVNL++IFGAARGGKSFLMNQLA R  VF+ISN KDPCT GVD+SR VL                          ADVEGQGDRDV++DARLAS++LP+AKCV+FNW+DSLQVDR      MLN L VM+ AA NV
Sbjct:   26 TGAVKWVTVEDGGVSPQ---LVVDPAAETVLQPLGDKVPVNLISIFGAARGGKSFLMNQLARRRDVFRISNAKDPCTTGVDLSRAVL-------------------------LADVEGQGDRDVSHDARLASSVLPLAKCVLFNWKDSLQVDRVLAVDRMLNQLAVMRHAALNV 171          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A2D4BS96_PYTIN (GBP domain-containing protein n=2 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BS96_PYTIN)

HSP 1 Score: 165 bits (418), Expect = 1.830e-48
Identity = 89/179 (49.72%), Postives = 119/179 (66.48%), Query Frame = 1
Query:   25 AMKWITM-----------ADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M           A  +GR    M V    A  VL+PLG  EP+NL++IFGAAR GKSFLMN LA +  +FKISN ++PCTQGVD+S   +PL  F+ + GC +   +S + V++ F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DR+LNLL V+ RAA  ++L
Sbjct:   15 AVKWLGMGRTNHQDDDDDASASGRVIDRMFVHPEAATEVLQPLG-DEPINLISIFGAARQGKSFLMNLLADQQDLFKISNLREPCTQGVDLSGHFIPLPTFSGLNGCPA---ISNNAVQIGFVDAEGQGDRDITYDSRLVSPVLLASKVVLFNWKDSLQADRILNLLAVLARAAQGIEL 189          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A3R6YGE9_9STRA (GBP domain-containing protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A3R6YGE9_9STRA)

HSP 1 Score: 166 bits (419), Expect = 4.010e-48
Identity = 88/168 (52.38%), Postives = 114/168 (67.86%), Query Frame = 1
Query:   25 AMKWITMADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M D  G+    M V   T+  VL  L  +  VNL++IFGAAR GKSFLMN LA +  +F+ISN+K+PCTQGVD+S   +PL  F+ I GC     VS   + + F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DRMLNLL V+ +AA NV+L
Sbjct:    3 AVKWLGM-DRDGK----MTVSPSTSLKVLHALPERHNVNLISIFGAARQGKSFLMNLLANQQDLFRISNEKEPCTQGVDLSSHFMPLESFSRINGCPP---VSSPNMSIGFVDAEGQGDRDITYDSRLVSPVLLASKVVIFNWKDSLQADRMLNLLAVLAKAAQNVEL 162          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A067BQ42_SAPPC (GBP domain-containing protein n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067BQ42_SAPPC)

HSP 1 Score: 173 bits (438), Expect = 9.420e-47
Identity = 94/168 (55.95%), Postives = 120/168 (71.43%), Query Frame = 1
Query:   25 AMKWITMADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M D  G+    MAV  P AE VLKPL   +P+NL++IFGAAR GKSFLMN LA +  +F+ISN+K+PCTQGVD+S   +PL EF+   G TS      S ++V F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DRMLNLL V+ +AA NV+L
Sbjct:    7 AVKWLGM-DRHGK----MAVA-PAAEQVLKPLPEAQPINLISIFGAARQGKSFLMNLLANQQDLFRISNEKEPCTQGVDLSSHFMPLAEFSRQNGNTSIP----SSMKVGFVDAEGQGDRDITYDSRLVSPVLLTSKVVIFNWKDSLQADRMLNLLAVLAKAAQNVEL 164          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A2P4XDC1_9STRA (Guanylate-binding protein n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4XDC1_9STRA)

HSP 1 Score: 171 bits (434), Expect = 1.720e-46
Identity = 88/168 (52.38%), Postives = 115/168 (68.45%), Query Frame = 1
Query:   25 AMKWITMADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M   AG+ D  M +    +  VL+PLG   P+NL++IFGAAR GKSFLMN LA +  +FKISN ++PCTQGVD+S   +PL  F+S  GC S    +   + V F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DRMLNLL V+ RAA  ++L
Sbjct:   17 AVKWLGMKHAAGKSDQ-MFISSEASTQVLEPLGNT-PINLISIFGAARQGKSFLMNLLADQQDLFKISNLREPCTQGVDLSGHFVPLSSFSSFNGCPSITPKASKGIHVGFVDAEGQGDRDITYDSRLVSPVLLSSKVVLFNWKDSLQADRMLNLLAVLARAAQGIEL 182          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: K3W7D1_GLOUD (GBP domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3W7D1_GLOUD)

HSP 1 Score: 171 bits (434), Expect = 3.280e-46
Identity = 89/172 (51.74%), Postives = 118/172 (68.60%), Query Frame = 1
Query:   25 AMKWITMADVAGRPDPI----MAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M    GR +      M V Q  A  VL+PLG  E +NL++IFGAAR GKSFLMN LA +  +FKISN ++PCTQGVD+S   +PL  F+++ GC      +G ++++ F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DRMLNLL V+ RAA  V+L
Sbjct:   18 AVKWLGMGPSDGRSNHTTSDHMFVSQEAATEVLQPLG-DEQINLISIFGAARQGKSFLMNLLADQQDLFKISNLREPCTQGVDLSGHFIPLSTFSALNGCPQVATQNGKQIQIGFVDAEGQGDRDITYDSRLVSPVLLSSKTVIFNWKDSLQADRMLNLLAVLARAAQGVEL 188          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: D0MZ80_PHYIT (GBP domain-containing protein n=14 Tax=Phytophthora TaxID=4783 RepID=D0MZ80_PHYIT)

HSP 1 Score: 171 bits (433), Expect = 4.450e-46
Identity = 89/169 (52.66%), Postives = 117/169 (69.23%), Query Frame = 1
Query:   25 AMKWITM-ADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M A  AG+ D  M +    +  VL+PLG   P+NL++IFGAAR GKSFLMN LA +  +FKISN ++PCTQGVD+S   +PL +F+S  GC S    +   + V F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DRMLNLL V+ RAA  ++L
Sbjct:   19 AVKWLGMGAHAAGKSDQ-MFISSEASTQVLQPLG-DTPINLISIFGAARQGKSFLMNLLADQQDLFKISNLREPCTQGVDLSGHFVPLSKFSSFNGCPSLASKASKNIHVGFVDAEGQGDRDITYDSRLVSPVLLASKVVIFNWKDSLQADRMLNLLAVLARAAQGIEL 185          
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Match: A0A6A3TSF2_9STRA (GBP domain-containing protein n=4 Tax=Phytophthora TaxID=4783 RepID=A0A6A3TSF2_9STRA)

HSP 1 Score: 171 bits (433), Expect = 4.480e-46
Identity = 89/168 (52.98%), Postives = 116/168 (69.05%), Query Frame = 1
Query:   25 AMKWITMADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGKSFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRGVSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDRMLNLLGVMQRAAHNVDL 528
            A+KW+ M   A      M +    AE VL+PLG   PVNL++IFGAAR GKSFLMN LA +  +FKISN ++PCTQGVD+S  +LPL  F+ + GC    G +G+ + V F D EGQGDRD+ YD+RL S +L  +K V+FNW+DSLQ DR+LNLL V+ RAA  ++L
Sbjct:   19 AVKWLGMGRHAAGKSDQMFISAEAAERVLQPLG-DTPVNLISIFGAARQGKSFLMNLLADQQDLFKISNLREPCTQGVDLSAHLLPLSRFSGLNGCPPVTG-TGTGIHVGFVDAEGQGDRDITYDSRLVSPVLLASKVVLFNWKDSLQADRILNLLAVLARAAQGIEL 184          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig10925.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LC12_ECTSI1.970e-7179.08GBP5, guanylate-binding protein (Divergent) n=1 Ta... [more]
A0A7S3URQ4_HETAK4.370e-5154.04Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A835YY61_9STRA1.850e-4958.05SAP domain-containing protein n=1 Tax=Tribonema mi... [more]
A0A2D4BS96_PYTIN1.830e-4849.72GBP domain-containing protein n=2 Tax=Pythium insi... [more]
A0A3R6YGE9_9STRA4.010e-4852.38GBP domain-containing protein n=1 Tax=Aphanomyces ... [more]
A0A067BQ42_SAPPC9.420e-4755.95GBP domain-containing protein n=2 Tax=Saprolegnia ... [more]
A0A2P4XDC1_9STRA1.720e-4652.38Guanylate-binding protein n=1 Tax=Phytophthora pal... [more]
K3W7D1_GLOUD3.280e-4651.74GBP domain-containing protein n=1 Tax=Globisporang... [more]
D0MZ80_PHYIT4.450e-4652.66GBP domain-containing protein n=14 Tax=Phytophthor... [more]
A0A6A3TSF2_9STRA4.480e-4652.98GBP domain-containing protein n=4 Tax=Phytophthora... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig10925contigC-tenellus_contig10925:411..1534 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D8LC12
PFAMsGBP
Model size531
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons3
Evalue2.27e-72
EggNOG OGsKOG2037@1|root,KOG2037@2759|Eukaryota
DescriptionGTPase activity
Cds size504
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789154.9931448-CDS-C-tenellus_contig10925:410..5981680789154.9931448-CDS-C-tenellus_contig10925:410..598Choristocarpus tenellus KU2346CDSC-tenellus_contig10925 411..598 -
1680789155.0073812-CDS-C-tenellus_contig10925:966..10721680789155.0073812-CDS-C-tenellus_contig10925:966..1072Choristocarpus tenellus KU2346CDSC-tenellus_contig10925 967..1072 -
1680789155.022757-CDS-C-tenellus_contig10925:1297..15071680789155.022757-CDS-C-tenellus_contig10925:1297..1507Choristocarpus tenellus KU2346CDSC-tenellus_contig10925 1298..1507 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789155.03723-UTR-C-tenellus_contig10925:1507..15341680789155.03723-UTR-C-tenellus_contig10925:1507..1534Choristocarpus tenellus KU2346UTRC-tenellus_contig10925 1508..1534 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig10925.1.1prot_C-tenellus_contig10925.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig10925 411..1507 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig10925.1.1

>prot_C-tenellus_contig10925.1.1 ID=prot_C-tenellus_contig10925.1.1|Name=mRNA_C-tenellus_contig10925.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=168bp
MKWITMADVAGRPDPIMAVIQPTAETVLKPLGRKEPVNLVAIFGAARGGK
SFLMNQLAGRDGVFKISNDKDPCTQGVDVSRTVLPLHEFASIGGCTSPRG
VSGSRVRVLFADVEGQGDRDVAYDARLASAILPMAKCVVFNWRDSLQVDR
MLNLLGVMQRAAHNVDLG
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mRNA from alignment at C-tenellus_contig10925:411..1534-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig10925.1.1 ID=mRNA_C-tenellus_contig10925.1.1|Name=mRNA_C-tenellus_contig10925.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=1124bp|location=Sequence derived from alignment at C-tenellus_contig10925:411..1534- (Choristocarpus tenellus KU2346)
GGGCGTCCAGAACTGGTAACAGGGGCGATGAAGTGGATCACGATGGCCGA TGTGGCAGGAAGACCAGACCCTATCATGGCTGTGATCCAACCGACTGCAG AGACCGTGCTGAAGCCCCTTGGAAGAAAAGAGCCCGTGAACTTGGTGGCT ATCTTCGGCGCAGCACGAGGCGGCAAGAGCTTCCTGATGAACCAGCTAGC AGGCAGAGACGGCGTTTTCAAAATTTCAAATGACAAGGTTGTGCCTAGAT GATGATGTTGTAAGCGCCGGCGACGAATTATTTATTGTGTTAGTTGAGGT CTCTCATCCTTTCCTGTGTAAGGCGACCTCCAGTGTCTGTCACCTGAAGG TGTAAATTCCCTTAATCAAATACTACGTGAGGAGGACATCCGTCTGGTGT TTGCTGGACAATGAAATGCTCCTTGCTTCCCTCACCACAATCTTCTTTCT CCCGTATTGCAGGATCCTTGCACCCAGGGTGTCGATGTGTCACGGACCGT GTTGCCATTGCACGAGTTTGCCTCCATTGGCGGGTGTACCTCCCCAAGGG GAGTCTCTGGTAGCAGAGGTATGTTTGGCATTTGACGAACACTTATCGTA GTTCCTTCCCCTTTTATACAGGAATGCCTACTATGGAATCTGCGGGTTTT TCCATATCATGATTTTGTGACTTTTGTCACCTTGCCCTGCTTTTCTTCGT ATATCCAATATTTTTGGGGCGGTCATGGTTCAACATCTACATCCACACCC ACACGCGAATAACTTCGCACCTGCTCATCTACCAGCTCGTTTTACTACCA CGGACCATCTGCAGGAGAAGTCGTGCAAATGAAGCCAATAAGTAGAAAAA CTTGTACTGAAGAATCACCACGAAAGATGTATCAGATCCTCCGTCTCCCC CACTTTATCCCTCGCCCTCTGTTCCCATGAACGAAGTGCGGGTGCTATTT GCGGATGTGGAGGGACAGGGTGACCGAGATGTTGCGTACGATGCCCGATT GGCCTCCGCCATCCTTCCCATGGCAAAGTGTGTGGTATTCAACTGGCGGG ACAGTCTTCAGGTTGACCGCATGCTCAACCTTCTTGGGGTGATGCAGAGG GCTGCTCATAATGTTGACCTCGGT
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Coding sequence (CDS) from alignment at C-tenellus_contig10925:411..1534-

>mRNA_C-tenellus_contig10925.1.1 ID=mRNA_C-tenellus_contig10925.1.1|Name=mRNA_C-tenellus_contig10925.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=504bp|location=Sequence derived from alignment at C-tenellus_contig10925:411..1534- (Choristocarpus tenellus KU2346)
ATGAAGTGGATCACGATGGCCGATGTGGCAGGAAGACCAGACCCTATCAT
GGCTGTGATCCAACCGACTGCAGAGACCGTGCTGAAGCCCCTTGGAAGAA
AAGAGCCCGTGAACTTGGTGGCTATCTTCGGCGCAGCACGAGGCGGCAAG
AGCTTCCTGATGAACCAGCTAGCAGGCAGAGACGGCGTTTTCAAAATTTC
AAATGACAAGGATCCTTGCACCCAGGGTGTCGATGTGTCACGGACCGTGT
TGCCATTGCACGAGTTTGCCTCCATTGGCGGGTGTACCTCCCCAAGGGGA
GTCTCTGGTAGCAGAGTGCGGGTGCTATTTGCGGATGTGGAGGGACAGGG
TGACCGAGATGTTGCGTACGATGCCCGATTGGCCTCCGCCATCCTTCCCA
TGGCAAAGTGTGTGGTATTCAACTGGCGGGACAGTCTTCAGGTTGACCGC
ATGCTCAACCTTCTTGGGGTGATGCAGAGGGCTGCTCATAATGTTGACCT
CGGT
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