mRNA_C-tenellus_contig10219.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig10219.1.1
Unique NamemRNA_C-tenellus_contig10219.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: D7G7T8_ECTSI (CRAL-TRIO domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7T8_ECTSI)

HSP 1 Score: 190 bits (482), Expect = 1.730e-55
Identity = 106/202 (52.48%), Postives = 136/202 (67.33%), Query Frame = 1
Query:    4 SSFRQLCMEVSVFVFCMLIAALGSGLAVASHSFAPSVRTMLTGR-EPSG---YNPRLKRCNASGMLSTASE-KGLVNAAAAMDRAAAAPLEREDKVPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQ-GVTVPVLLRHWIFCAEYMWRVVHP 591
            SS   +C+   VF FC++++AL S + VA+     ++R  L    EPS    +NPRL   +AS  L   SE   ++ AA AMDRAAAAPL  E K+P R++RAFCGN   A ERWK TL WR+EN++D ILNEPQ +F  I+ +FPS M   GRSR GH+VVYDKL GVDM  +  + GVTVP+LLRHWIF +EYM+RV+ P
Sbjct:   37 SSQNMVCLPPHVFAFCIVMSALVSAIVVANQRIYGAIREYLRQLLEPSASRVHNPRLLHRSASHQLDKDSEGANVIEAAEAMDRAAAAPLALEHKIPARYVRAFCGNEKHALERWKATLKWRKENEIDAILNEPQQFFHDIRRYFPSHMS--GRSRGGHIVVYDKLGGVDMRTLRRKLGVTVPMLLRHWIFVSEYMYRVLQP 236          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A6H5LAB2_9PHAE (CRAL-TRIO domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LAB2_9PHAE)

HSP 1 Score: 186 bits (471), Expect = 6.220e-52
Identity = 104/202 (51.49%), Postives = 135/202 (66.83%), Query Frame = 1
Query:    4 SSFRQLCMEVSVFVFCMLIAALGSGLAVASHSFAPSVRTMLTGR-EPSG---YNPRLKRCNASGMLSTASEKG-LVNAAAAMDRAAAAPLEREDKVPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQ-GVTVPVLLRHWIFCAEYMWRVVHP 591
            SS   +C+   VF FC++++AL S + VA+     ++R  L    EPS    +NPRL   +AS  L   SE   ++ AA AMDRAAAAPL  E K+P R++RAFCGN   A ERWK TL WR+EN++D ILNEPQ +F  I+ +FPS M   GRSR GH+VVY+KL  VDM  +  + GVTVP+LLRHWIF +EYM+RV+ P
Sbjct:   35 SSQNMVCLPPHVFAFCIVMSALVSAIVVANQRIYGAIREYLRQLLEPSASRVHNPRLLHRSASHQLDKDSEGAHVIEAAEAMDRAAAAPLALEHKIPARYVRAFCGNEKHALERWKATLKWRKENEIDAILNEPQQFFHDIRRYFPSHMS--GRSRGGHIVVYEKLGAVDMRTLRRKLGVTVPMLLRHWIFVSEYMYRVLQP 234          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A7S4DBX9_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBX9_HETAK)

HSP 1 Score: 112 bits (281), Expect = 7.870e-26
Identity = 49/119 (41.18%), Postives = 77/119 (64.71%), Query Frame = 1
Query:  235 DRAAAAPLEREDKVPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP 591
            D  A AP      VP +F+     ++ +A  RW  T  WR +  +DTIL+EPQP+F +IK+H+P FMC  GR++ G++V Y++L  +D+ A+A  G+TV   +RH++F  EY+WR++ P
Sbjct:  117 DGEAMAPASALPPVPRKFVDGCFQDMVEAKRRWAMTHRWRVKEKIDTILDEPQPHFKVIKQHYPHFMC--GRAKSGNIVYYERLGEIDLGAIAASGITVDQCVRHYVFITEYLWRILSP 233          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A485K5A0_9STRA (Aste57867_436 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485K5A0_9STRA)

HSP 1 Score: 108 bits (270), Expect = 1.570e-25
Identity = 46/113 (40.71%), Postives = 74/113 (65.49%), Query Frame = 1
Query:  253 PLEREDKVPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP 591
            P+  E    PRF+ A  G+  K   RW+ T  WRREN++D +L+EPQP+F IIK+++P +   +G+S+ GH V Y++L  VD   + + G+ +  LLRH++F  E++W+V+ P
Sbjct:   21 PVIEEIPFSPRFIAAEKGDEEKGRHRWEATRQWRRENNIDGLLHEPQPHFDIIKKNYPQYF--HGKSKNGHCVYYERLGYVDFKTLKKSGLNMDQLLRHYMFITEFLWKVLEP 131          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A3R6Z8R0_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A3R6Z8R0_9STRA)

HSP 1 Score: 112 bits (279), Expect = 2.840e-25
Identity = 47/104 (45.19%), Postives = 74/104 (71.15%), Query Frame = 1
Query:  280 PRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP 591
            PRF+ A  GN+ K   R+++TL WRREN+VD +L EPQP+F +IK+++P +   +G+S +GH V Y+K+  +D+ AM   G+T+  LLRH+++  EYMW ++ P
Sbjct:  320 PRFIAAEKGNVEKGRARYESTLQWRRENNVDGMLQEPQPHFHLIKKYYPQYF--HGKSIKGHCVYYEKVGKIDLKAMKAAGLTIDQLLRHYVYLTEYMWTILEP 421          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A024UU13_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UU13_9STRA)

HSP 1 Score: 112 bits (279), Expect = 3.910e-25
Identity = 47/104 (45.19%), Postives = 74/104 (71.15%), Query Frame = 1
Query:  280 PRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP 591
            PRF+ A  GN+ K   R+++TL WRREN+VD +L EPQP+F +IK+++P +   +G+S +GH V Y+K+  +D+ AM   G+T+  LLRH+++  EYMW ++ P
Sbjct:  476 PRFIAAEKGNVEKGRARYESTLQWRRENNVDGMLQEPQPHFHLIKKYYPQYF--HGKSIKGHCVYYEKVGKIDLKAMKAAGLTIDQLLRHYVYLTEYMWTILEP 577          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A7S3Y7R1_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y7R1_HETAK)

HSP 1 Score: 111 bits (278), Expect = 4.020e-25
Identity = 51/113 (45.13%), Postives = 74/113 (65.49%), Query Frame = 1
Query:  241 AAAAPLEREDKVPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWR 579
            AAA P      +P R++R   G+L +A  RW  TL WRRE +VD IL+EPQPYF +IK+++P F   Y R R G V  Y+++   D+ AM  +GVT+  L+RH++F +E++WR
Sbjct:  149 AAAGPTAGGAALPDRYIRGCDGDLEEARRRWALTLRWRREQNVDGILDEPQPYFFVIKKYYPHF--TYKRGRNGCVTYYEQVGRADIEAMRARGVTIAALVRHYVFVSEFLWR 259          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A024USX5_9STRA (CRAL-TRIO domain-containing protein n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024USX5_9STRA)

HSP 1 Score: 106 bits (265), Expect = 8.210e-25
Identity = 43/104 (41.35%), Postives = 76/104 (73.08%), Query Frame = 1
Query:  280 PRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP 591
            PRF+ A  GN  K  +R+++TL+WRR+N +DT+++EPQ +F +IK+++P +   +G+S+ GH V Y+KL  V+  A+ + G+++  LLRH+++  EY+W+V+ P
Sbjct:   27 PRFIAAEKGNAEKGRQRYESTLSWRRDNRIDTLIDEPQVHFHLIKKNYPQYF--HGKSKNGHRVYYEKLGSVNFKALKKSGLSMDQLLRHYMYITEYLWKVLDP 128          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A7S1UKD6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UKD6_9STRA)

HSP 1 Score: 108 bits (271), Expect = 3.790e-24
Identity = 44/109 (40.37%), Postives = 75/109 (68.81%), Query Frame = 1
Query:  265 EDKVPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP 591
            E  +P +F+   C  L +AS RW  T  WR++  +D +L+EPQP+F+ IK ++P F C  GR+R G VV Y+++  +D+ A+ ++G+++ +L+RH++F  E+MWR+ HP
Sbjct:  236 EQAIPVQFIEG-CPPLTEASRRWDITAKWRKQEGIDDMLDEPQPHFNNIKRYYPHFCC--GRTRDGDVVYYEQVGNIDLAALRDRGLSIDLLVRHYLFTTEFMWRMTHP 341          
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Match: A0A7S2RPQ3_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RPQ3_9STRA)

HSP 1 Score: 99.4 bits (246), Expect = 8.310e-23
Identity = 43/102 (42.16%), Postives = 68/102 (66.67%), Query Frame = 1
Query:  274 VPPRFLRAFCGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGRSRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWR 579
            VP R LR   G+  +A  RW  T  WRRE +VD++L+EPQP F +IK  +P F+  +G++R G  +  +++  +D+ A+   GVTVPVL+RH++F +E++W 
Sbjct:   74 VPGRVLRGCDGDPVEAKRRWALTCIWRREYNVDSVLSEPQPCFHVIKRCYPHFL--HGKARGGQAIYIEQVGRIDVQALLRHGVTVPVLMRHYVFNSEFLWN 173          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig10219.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G7T8_ECTSI1.730e-5552.48CRAL-TRIO domain-containing protein n=1 Tax=Ectoca... [more]
A0A6H5LAB2_9PHAE6.220e-5251.49CRAL-TRIO domain-containing protein n=1 Tax=Ectoca... [more]
A0A7S4DBX9_HETAK7.870e-2641.18Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A485K5A0_9STRA1.570e-2540.71Aste57867_436 protein n=1 Tax=Aphanomyces stellatu... [more]
A0A3R6Z8R0_9STRA2.840e-2545.19Uncharacterized protein (Fragment) n=1 Tax=Aphanom... [more]
A0A024UU13_9STRA3.910e-2545.19Uncharacterized protein n=1 Tax=Aphanomyces invada... [more]
A0A7S3Y7R1_HETAK4.020e-2545.13Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A024USX5_9STRA8.210e-2541.35CRAL-TRIO domain-containing protein n=2 Tax=Aphano... [more]
A0A7S1UKD6_9STRA3.790e-2440.37Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A7S2RPQ3_9STRA8.310e-2342.16Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig10219contigC-tenellus_contig10219:5..2024 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7G7T8
PFAMsCRAL_TRIO
Model size591
Max annot lvl2759|Eukaryota
Hectar predicted targeting categorysignal peptide
Exons4
Evalue2.84e-56
EggNOG OGsKOG1471@1|root,KOG1471@2759|Eukaryota
Ec32 ortholog descriptionCRAL-TRIO domain
Ec32 orthologEc-06_000330.1
Descriptionmacromolecule localization
Cds size591
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789121.4465978-CDS-C-tenellus_contig10219:4..501680789121.4465978-CDS-C-tenellus_contig10219:4..50Choristocarpus tenellus KU2346CDSC-tenellus_contig10219 5..50 -
1680789121.4610639-CDS-C-tenellus_contig10219:484..5891680789121.4610639-CDS-C-tenellus_contig10219:484..589Choristocarpus tenellus KU2346CDSC-tenellus_contig10219 485..589 -
1680789121.4778872-CDS-C-tenellus_contig10219:1111..13321680789121.4778872-CDS-C-tenellus_contig10219:1111..1332Choristocarpus tenellus KU2346CDSC-tenellus_contig10219 1112..1332 -
1680789121.4878716-CDS-C-tenellus_contig10219:1805..20241680789121.4878716-CDS-C-tenellus_contig10219:1805..2024Choristocarpus tenellus KU2346CDSC-tenellus_contig10219 1806..2024 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig10219.1.1prot_C-tenellus_contig10219.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig10219 5..2024 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig10219.1.1

>prot_C-tenellus_contig10219.1.1 ID=prot_C-tenellus_contig10219.1.1|Name=mRNA_C-tenellus_contig10219.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=197bp
MSSFRQLCMEVSVFVFCMLIAALGSGLAVASHSFAPSVRTMLTGREPSGY
NPRLKRCNASGMLSTASEKGLVNAAAAMDRAAAAPLEREDKVPPRFLRAF
CGNLNKASERWKTTLAWRRENDVDTILNEPQPYFSIIKEHFPSFMCVYGR
SRQGHVVVYDKLAGVDMHAMAEQGVTVPVLLRHWIFCAEYMWRVVHP
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mRNA from alignment at C-tenellus_contig10219:5..2024-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig10219.1.1 ID=mRNA_C-tenellus_contig10219.1.1|Name=mRNA_C-tenellus_contig10219.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=2020bp|location=Sequence derived from alignment at C-tenellus_contig10219:5..2024- (Choristocarpus tenellus KU2346)
ATGTCCTCCTTCCGCCAGCTATGTATGGAGGTCTCGGTATTTGTCTTCTG TATGCTGATAGCTGCCTTGGGTTCTGGCCTGGCAGTCGCCAGCCACAGCT TCGCACCGTCAGTCCGTACCATGCTGACTGGACGAGAGCCGAGTGGTTAC AACCCACGTCTAAAGAGATGCAACGCGTCGGGCATGCTCTCCACTGCGTC GGAGAAGGGTCTGGTCAATGTGAGGGAGGGAGCATATGGGCATTGAATTA TGTTAAAGCAGGAAAATATATATCGAGATAGATGATACGTCAGTACCAAG CCACGAGTGCGTTCCTGCTGGTGCGAGTCCATAACCTAACTGACCCAAAG TCTGCCTCTTGTGGTTCTCTCCATGTATGGATAGATGCTCTTCACAATCG ATGGGATAGTTTTATACTCAACTGTGAGAGGGTCCTACACTATTTCCCCC GAAAATTTCCTGTTGTGTGTTGTGCAAAGACCGATCTACATCGTCCCTGA CGTGACAATCACCTTCTTCCATGTATCTACCTCCGCCGCTTTTCTTTTCC CCCTGATAAGCTGCTTACATAATCAAACGCATGGTAGCCACAACTATCGA CGATCAAGGTTCCATCTGGTCTCTTGGCATTGCCATCCATTACCCACAAC CCCACCCCCTCCCACCCCTTTGGTCACACACACACACGGCAGGCTGCAGC TGCAATGGACCGTGCAGCAGCGGCTCCCCTGGAACGCGAAGATAAAGTAC CTCCCCGCTTTTTGCGAGCTTTCTGTGGCAACCTCAACAAGGCCTCAGAG AGGTGGAAGACAACACTTGCATGGAGAAGGGAGAACGATGTGGATACCAT CCTAAATGAGCCTCAACCGTACTTCTCGATTATCAAAGAGCACTTTCCCT CGTTCATGTGCGTGTCTTTGGCTAAACAAGCAGAAGGACTGGGGTGCACT TCGTGGGAGTGCATATTGTGATTGCTGTTTGGGTGTGGTGGTGGTGTGGT GGGTTGTCTGAAGTCATTGTTGAAGGTCTTTTCTTTTTTTTATTTCTGTG TAGAAGTTGCTTCAGTTGATGTGTCTAGAATCCTCAATTAAAAAAAGGTC TTTAGTTCTCCAACCTTGCAACTGTACGCCATCACCTCCCTAGTGCTATT TATCTATTGTCAGTTTTGGATCAACAATGGAATCTTCGCATCACTGACAT CTGGGTGAAGAATTGAGGTTTACAAGGATACAGTGGATGGGAAAGGGATA CAACAGATCTGATTCACATGAAATCTGGGTCGCACCGTGTATTTGAGTGG ACTCTGAAAAGGTAGAATATGGAAGGTTTGCAGGAAATCATTTGTTTGAT GAGTGGGCAAAGGCATCGTATCTTCAAACTGTACTCAACATATTTCCTTG CCCTCCCTCTATCACACATGCAAACGCCTCTACAGGTATGGGAGGAGTAG GCAAGGCCATGTTGTGGTGTATGACAAGCTGGCGGGGGTCGACATGCATG CCATGGCTGAGCAAGGTGTTACAGTCCCTGTTCTTCTGAGGTGAAAAGAG TTTGTGTGTGTTAAATTTGTTTTGTACAGGGTATGGTGCTTGTTGTGAGC TCCAGGGGCTTTAGGGTACATCTGGAGATGGACTGTGGGGTACAATCAGA AGGGTTTATTTAGGTGGGGATCTGCACCGGGGACTGTCATGAGTGTGTGA GGTCACAAGTTGGCATTTGTGCTACACGTTTCTTTGCATTCCACATGTTT AGTGTTGAAAAGTCTGAGTCAGGTCTTCTGTCATACTCTTGAACTCTCGC TCCCCCAGTTATACTCTTTTTTTTTTCCATTTCGCGTCCGATCGTTGGTT ATCTTGTGCTGTGGCACCTGCCCATTTTCTTGGTTTAGTCAAGTCATCAA CCTTTCTTCCTCTCTCGAATTTTTGTGTGAACCCCCCCTCTTTCTCGACT TTTCTCCTTTACTACTTGTGGTAGGCACTGGATCTTCTGTGCGGAGTACA TGTGGCGGGTGGTGCACCCT
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Coding sequence (CDS) from alignment at C-tenellus_contig10219:5..2024-

>mRNA_C-tenellus_contig10219.1.1 ID=mRNA_C-tenellus_contig10219.1.1|Name=mRNA_C-tenellus_contig10219.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=591bp|location=Sequence derived from alignment at C-tenellus_contig10219:5..2024- (Choristocarpus tenellus KU2346)
ATGTCCTCCTTCCGCCAGCTATGTATGGAGGTCTCGGTATTTGTCTTCTG
TATGCTGATAGCTGCCTTGGGTTCTGGCCTGGCAGTCGCCAGCCACAGCT
TCGCACCGTCAGTCCGTACCATGCTGACTGGACGAGAGCCGAGTGGTTAC
AACCCACGTCTAAAGAGATGCAACGCGTCGGGCATGCTCTCCACTGCGTC
GGAGAAGGGTCTGGTCAATGCTGCAGCTGCAATGGACCGTGCAGCAGCGG
CTCCCCTGGAACGCGAAGATAAAGTACCTCCCCGCTTTTTGCGAGCTTTC
TGTGGCAACCTCAACAAGGCCTCAGAGAGGTGGAAGACAACACTTGCATG
GAGAAGGGAGAACGATGTGGATACCATCCTAAATGAGCCTCAACCGTACT
TCTCGATTATCAAAGAGCACTTTCCCTCGTTCATGTGCGTGTATGGGAGG
AGTAGGCAAGGCCATGTTGTGGTGTATGACAAGCTGGCGGGGGTCGACAT
GCATGCCATGGCTGAGCAAGGTGTTACAGTCCCTGTTCTTCTGAGGCACT
GGATCTTCTGTGCGGAGTACATGTGGCGGGTGGTGCACCCT
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