mRNA_C-linearis_contig10.449.1 (mRNA) Chordaria linearis ClinC8C monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-linearis_contig10.449.1
Unique NamemRNA_C-linearis_contig10.449.1
TypemRNA
OrganismChordaria linearis ClinC8C monoicous (Chordaria linearis ClinC8C monoicous)
Homology
[Close]
BLAST of mRNA_C-linearis_contig10.449.1 vs. uniprot
Match: A0A6H5KPZ6_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KPZ6_9PHAE)

HSP 1 Score: 100 bits (249), Expect = 7.310e-20
Identity = 56/99 (56.57%), Postives = 67/99 (67.68%), Query Frame = 2 
Query:  353 NTDVLSHLLDFVAEDQYLFFAAVSRAWTSVWRAAQRPAATRQVTPDTSESQLRESLEAGLPLDKAQLSAALARHGHLELLQLARERGCAWGAVTCSAAA 649
            N D+L  ++ FVAE Q+LFFA VSRAW   W    RPA T  VT D+S SQLR S E GLP D+ +L AA+AR G+LELL+ AR  GCAW  +T S AA
Sbjct:   60 NVDLLRSIVRFVAEKQFLFFAPVSRAWREAWGC--RPAVTSWVTEDSSVSQLRYSFERGLPRDRPELCAAIARLGNLELLRSARGSGCAWNGLTFSEAA 156
[Close]
BLAST of mRNA_C-linearis_contig10.449.1 vs. uniprot
Match: D8LDD9_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LDD9_ECTSI)

HSP 1 Score: 92.4 bits (228), Expect = 4.420e-17
Identity = 49/97 (50.52%), Postives = 63/97 (64.95%), Query Frame = 2 
Query:  359 DVLSHLLDFVAEDQYLFFAAVSRAWTSVWRAAQRPAATRQVTPDTSESQLRESLEAGLPLDKAQLSAALARHGHLELLQLARERGCAWGAVTCSAAA 649
            DV+SH  +FV +DQ+LFFA+VSRAW   WR   RP +TR +TPDTS SQLR S++ GL      +  A+A  G L+LL+ AR  G  W  +TC  AA
Sbjct:   10 DVISHASEFVHDDQFLFFASVSRAWRGAWRG--RPTSTRAITPDTSPSQLRHSVDCGLSPGGTAVCTAIASLGRLDLLRAARALGFRWDKMTCWKAA 104
[Close]
BLAST of mRNA_C-linearis_contig10.449.1 vs. uniprot
Match: D7FH77_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FH77_ECTSI)

HSP 1 Score: 83.2 bits (204), Expect = 6.820e-14
Identity = 43/103 (41.75%), Postives = 60/103 (58.25%), Query Frame = 2 
Query:  326 PTAVGLAITNTDVLSHLLDFVAEDQYLFFAAVSRAWTSVWRAAQRPAATRQVTPDTSESQLRESLEAGLPLDKAQLSAALARHGHLELLQLARERGCAWGAVT 634
            P  V   + N D+L  L+ FVA++ +LFF  V R W + W    RP  TR  T D + +QL+   + GL     +L AA+AR G ++LL+LAR+ GC WGA T
Sbjct:    3 PGIVPSVLNNPDILGCLISFVADESFLFFGPVCRDWRAAW--GNRPTTTRIATSDLTPTQLQRGFDCGLTRTNPELCAAIARLGDVDLLELARDNGCRWGAST 103
[Close]
BLAST of mRNA_C-linearis_contig10.449.1 vs. uniprot
Match: A0A6H5L0J7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0J7_9PHAE)

HSP 1 Score: 73.9 bits (180), Expect = 2.980e-11
Identity = 42/93 (45.16%), Postives = 56/93 (60.22%), Query Frame = 2 
Query:  359 DVLSHLLDFVAEDQYLFFAAVSRAWTSVWRAAQRPAATRQVTPDTSESQLRESLEAGLPLDKAQLSAALARHGHLELLQLARERGCAWGAVTC 637
            D++S + +FV ++QYLFFA+VSR W  VW   +RP  T+ +T DTS  QL  S E GL    A +   +A  G L+LL+ AR  GC W   TC
Sbjct:    8 DIVSTVAEFVDDNQYLFFASVSRGWRDVW--GKRPTVTKALTADTSVPQLSLSFECGLRRTAA-VCETVAGLGRLDLLRRARAMGCQWNENTC 97
[Close]
BLAST of mRNA_C-linearis_contig10.449.1 vs. uniprot
Match: D7FMS7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMS7_ECTSI)

HSP 1 Score: 74.7 bits (182), Expect = 8.270e-11
Identity = 43/93 (46.24%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:  359 DVLSHLLDFVAEDQYLFFAAVSRAWTSVWRAAQRPAATRQVTPDTSESQLRESLEAGLPLDKAQLSAALARHGHLELLQLARERGCAWGAVTC 637
            D++S + +FV ++QYLFFA+VSR W  VW   +RP  T+ +T DTS SQL  S E GL    A +   +A  G L+LL+ AR  GC W   TC
Sbjct:   10 DIVSTVGEFVDDNQYLFFASVSRGWRDVW--GKRPTVTKALTADTSVSQLSLSFECGLRRTAA-VCETVAGLGRLDLLRRARAMGCPWNENTC 99
The following BLAST results are available for this feature:
BLAST of mRNA_C-linearis_contig10.449.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
A0A6H5KPZ6_9PHAE7.310e-2056.57Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
D8LDD9_ECTSI4.420e-1750.52Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
D7FH77_ECTSI6.820e-1441.75Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A6H5L0J7_9PHAE2.980e-1145.16Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FMS7_ECTSI8.270e-1146.24Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-linearis_contig10contigC-linearis_contig10:408903..412497 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Chordaria linearis ClinC8C monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score68.9
Seed ortholog evalue4.7e-09
Seed eggNOG ortholog2880.D7FJY0
KEGG koko:K06052
KEGG Pathwayko01522,ko04330,ko04371,ko04658,ko04668,ko05165,ko05200,ko05224,map01522,map04330,map04371,map04658,map04668,map05165,map05200,map05224
Hectar predicted targeting categoryother localisation
EggNOG OGs2AMK4@1,2RZCC@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04090
Exons2
Model size1801
Cds size954
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-linearis_contig10.449.1prot_C-linearis_contig10.449.1Chordaria linearis ClinC8C monoicouspolypeptideC-linearis_contig10 409506..412253 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927322.2566488-UTR-C-linearis_contig10:408902..4095051622927322.2566488-UTR-C-linearis_contig10:408902..409505Chordaria linearis ClinC8C monoicousUTRC-linearis_contig10 408903..409505 -
1690879625.7625678-UTR-C-linearis_contig10:408902..4095051690879625.7625678-UTR-C-linearis_contig10:408902..409505Chordaria linearis ClinC8C monoicousUTRC-linearis_contig10 408903..409505 -
1622927322.2926915-UTR-C-linearis_contig10:412253..4124971622927322.2926915-UTR-C-linearis_contig10:412253..412497Chordaria linearis ClinC8C monoicousUTRC-linearis_contig10 412254..412497 -
1690879625.7958827-UTR-C-linearis_contig10:412253..4124971690879625.7958827-UTR-C-linearis_contig10:412253..412497Chordaria linearis ClinC8C monoicousUTRC-linearis_contig10 412254..412497 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927322.2664757-CDS-C-linearis_contig10:409505..4104171622927322.2664757-CDS-C-linearis_contig10:409505..410417Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 409506..410417 -
1690879625.7757072-CDS-C-linearis_contig10:409505..4104171690879625.7757072-CDS-C-linearis_contig10:409505..410417Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 409506..410417 -
1622927322.2806287-CDS-C-linearis_contig10:412211..4122531622927322.2806287-CDS-C-linearis_contig10:412211..412253Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 412212..412253 -
1690879625.7851439-CDS-C-linearis_contig10:412211..4122531690879625.7851439-CDS-C-linearis_contig10:412211..412253Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 412212..412253 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-linearis_contig10.449.1

>prot_C-linearis_contig10.449.1 ID=prot_C-linearis_contig10.449.1|Name=mRNA_C-linearis_contig10.449.1|organism=Chordaria linearis ClinC8C monoicous|type=polypeptide|length=318bp
MGPQQQGAGDGLAEGTASSSTDGAPSAPTAVGLAITNTDVLSHLLDFVAE
DQYLFFAAVSRAWTSVWRAAQRPAATRQVTPDTSESQLRESLEAGLPLDK
AQLSAALARHGHLELLQLARERGCAWGAVTCSAAAGAGHLELLQWCKYNG
CPWDDRTLTEAARGDHAEVLEWARMFGCEVSMGVPTAAAGAGNLNLLKYL
IQDGFPVCAGACGAAAKSGHLEILEYLVENECPVDEMTLFGVVLNGQLAV
VEWALGEGVEFNDLGEYAFCDAVKKGRLAVVRLLHRHGCPWDEASCLAAA
IEGGHEEIASWIRENRS*
back to top

mRNA from alignment at C-linearis_contig10:408903..412497-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-linearis_contig10.449.1 ID=mRNA_C-linearis_contig10.449.1|Name=mRNA_C-linearis_contig10.449.1|organism=Chordaria linearis ClinC8C monoicous|type=mRNA|length=3595bp|location=Sequence derived from alignment at C-linearis_contig10:408903..412497- (Chordaria linearis ClinC8C monoicous)
CCTCTCTCGTGAGTCTCTTGTGCCGCTCACATATCAGTCGACTAGGACCC
TACGCTTCATCCAAGCGAACGACCTGTCCCTTCTTGATTCAGCGGGGCAG
CAGGCAGCCCCTGCGAACCAGGGAGGAGAGATGTCGACTGGTGTCTAGCG
GAAATCTCGCTCTTGTCCGCACACACACACACACGACACACAGGATCTAG
ACTACTTCTAGACAGCAGTAGAGAGCTGGGTTTGGAGATTGGAGATGGGG
CCACAGCAACAAGGAGCCGGAGACGGGTTAGCAGAGGTGAGTCGGTACTG
GAATGGTACTTCGACAATCCCTCAACCGTGGTGGCTGTGTATTGTCATCT
CCGTCGCTGCAGAGAGCAGGAGCCCGCGACACATGAATCCTGAATCCGAG
TTGGAGTTGCGCCGACGTTTTGGAGGTGCATCCGCTCCACAACCTCCTAG
CAAAAGCACCACCGCCGCATGGCATTGCCACACAACCTTTCAAGCGTGAC
ACTTTGTTGATTGTTAGCTCGAGCCAGGGGAGAGCTGTCATATGGTAGAA
CGGCCGCTCGCCGCAGCCGAGCAACAACTTCAAACAATCACACCACAGCA
AGCAGTCGTCGTGCCGTCTACCAGGTGTCGTCTACTGCTGTACCTATGTA
TGTACCAGCACCAAGATCCATAGATCCCGAGACTACAGTGGTAGACTGGT
TACTTCGTACAGTGCCAATATATCGCTGACAACGGCACAGGCGCGCCACA
CTTCCTTTCTTTGAGGGACTGATGGAGTCGCTGGGTTGGGTACCCCGCCG
ATGATCACCCGTGAGTATTAGAAACAGCAAGCAGCGTTGTGGGTTTGTTG
CGGCCACCGATTTTTAGGGGCACACGCGTTCGTTACTGCGTTGTCCTGGG
GTATACGTGTATGAGTCTAGTCTGCGTTGGGTTAGCGTCGACACGCGCCC
CTCCTCTCGATCGTCTCTTGAGAGCTTAATCCAGAGCTTTGCACAGCATC
TGCTTATCTTCTGACCAAGCACCTAACTTCCAGGAGGACGCCGCATCGAA
CTCGGACGGTCAGTTCGTGATCTTTGTCTTGTCATCTGCACGATGCTATA
GAGCATCAAGGTCCGACACATTACACATCCTCGGTCTAGACTGTTGGTGT
GCCTCGAGCAGTACACGTAGTTATTGTCTTTCTTTCAATCATGTCATGAA
GACCCCAAGCGACAACCACGAGACCGTAACAGGTTGCCTTGAGTTTCGCC
GGAGTTTCGAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCATACAC
ATCTCTCCCATACACACCCATAACAAAGAGGTGACCGGCTTACTCAGAAC
CGTACCACTTGCATCGAATGGGGATCTTGTAGCAGTCAGGACAGGACAGC
AGTATCTAGTCGACCGCGGCCGGTGCTTGTTGCTTCGCCCTGTTGAGTTT
GCGAGGCATCACCGGTATGCGCCCCAGTGTCGTGAGTCTGAATCTATCCA
CTCTGTGCGGAATGTACATATATACAGCACTTGCACACCACACCACCGCA
CCACGTTCGAAAAACGCTTGGAGTTTCGCCCTCGGGCGTCGGGCAGCGTT
TGGTGCCTGCCTGGTATTTTCGATGAGAATAAACTGGGAGCGCCCGTTTG
ACGTTCACACACACGGTGCCCCCGGCTTCTTTTTCGTCCTCTGTTGCCCC
ACGCGTGTAGATGGGGAGGCACGGCTCACCTTAATTCGATTTCAAAAACA
TAATCCCCGCATAGTGCCAGCCAGCGGTACAACACCTCCGATTCGACCAA
TCTTATCCCCAAGAGCTCCCCTGGGCCCCCGCTGGCAAGAAACGTATGAC
TCGCGGCTCGATTGAGTTTTCCGTCACCACTTTTCGAACGTAGCGCTTGG
GATAAGGGTTGAATGTAAGATCCACGATCGAGCCACATGCATGCTTCTTA
GCCCAACTTTCTTATTTTTCTTATTTTCTTACGACAGTTTCTCTTTTACA
GCGGGCGCCCTCCGTACTGCGAACACCTTCCTTTCGGCTCGTTTGCCCCC
TTCCCTCAAAACGAATCGGGCACCAAGCAGGGCACCGCCAGCTCATCCAC
CGATGGAGCACCAAGCGCCCCAACAGCAGTCGGCCTCGCCATCACCAACA
CGGACGTTTTGAGCCACCTCCTAGACTTCGTGGCAGAGGACCAATATCTC
TTCTTCGCCGCGGTCTCGCGAGCCTGGACGTCGGTGTGGAGGGCTGCCCA
ACGCCCCGCGGCCACGAGGCAAGTCACGCCAGACACGTCCGAGTCTCAGC
TGCGAGAGAGTCTCGAGGCCGGCTTGCCTCTAGACAAGGCACAGCTGTCG
GCAGCCCTCGCACGCCACGGGCATCTAGAGCTGCTGCAGCTGGCCAGGGA
GCGCGGCTGCGCGTGGGGCGCGGTGACCTGTTCCGCCGCCGCCGGGGCGG
GACACCTGGAGCTCCTCCAGTGGTGCAAGTACAACGGGTGCCCATGGGAC
GACCGCACGCTGACAGAGGCCGCGCGCGGCGACCACGCGGAGGTCCTGGA
GTGGGCGAGGATGTTCGGCTGCGAGGTGAGCATGGGCGTCCCCACCGCGG
CGGCGGGGGCGGGGAACCTGAACCTCCTCAAGTACTTGATCCAGGACGGG
TTTCCGGTTTGTGCGGGGGCTTGCGGCGCGGCGGCTAAATCCGGCCACCT
GGAAATCCTGGAGTACTTGGTCGAGAACGAGTGCCCTGTCGACGAGATGA
CGCTCTTTGGGGTCGTTCTCAACGGCCAGCTAGCTGTCGTGGAGTGGGCG
CTCGGGGAAGGGGTCGAGTTTAACGACCTCGGGGAGTACGCCTTCTGCGA
CGCCGTGAAGAAGGGCCGATTGGCGGTGGTCCGTCTTCTGCATCGGCACG
GGTGTCCGTGGGACGAGGCGAGCTGTTTGGCGGCGGCGATCGAGGGTGGT
CACGAGGAAATCGCTAGCTGGATAAGGGAGAACCGATCGTAAACGTTCGG
GCTTGCATCTTGCGTGTGTGTTTGTGGCCTTCAAAAACAGATGGTACTGC
ACACGAGATTATTAGCATTTTGTCTGCAGATTTTAACTTTTCTCGCAAGG
CAACTTCATACTCCCATAAAAGCCGGTGTTCGGGCGGACCGAGCGTGTTG
CCTGTCTCTCTTGCTTTCTCCCGTCACGCGCCACAAGGGTTTGCTTCTGA
GCTTTATTGACTCGTTCAAGCGGCGTGCCTGCTTGCCCTGTTGGTTGCAA
CCACCCTGTAGCTTTTGTCCAAGTACTTCAAAGCCTGCGTTTTTTTGCCT
GCTGGTTGCCAATCTTTCGCTGGGAGTTTTCAACGATTGCGTGGGTGGTA
AGGATATCTGTTGTGGGTGCAGCAGGGTCTTGACGTTGAGATGCTTTCCG
TCAGTGCTCGCCTTCACGGCACGGTTGGAGGTTGGGGATAAAACCTACGG
GTGGATGCACCGACCACACAACTATTGGCTCTTCCTTTTTGTTTTCATGT
AGTATGTTTTGCCCGTTTGGTACAGCTTTGCGTCGAGAACTTCCTCCGGG
ATCGGAACGTTTGTCGCCCCCGGTGCAATTCAAGGCGCCACTTTC
back to top

Coding sequence (CDS) from alignment at C-linearis_contig10:408903..412497-

>mRNA_C-linearis_contig10.449.1 ID=mRNA_C-linearis_contig10.449.1|Name=mRNA_C-linearis_contig10.449.1|organism=Chordaria linearis ClinC8C monoicous|type=CDS|length=1908bp|location=Sequence derived from alignment at C-linearis_contig10:408903..412497- (Chordaria linearis ClinC8C monoicous)
ATGGGGCCACAGCAACAAGGAGCCGGAGACGGGTTAGCAGAGATGGGGCC
ACAGCAACAAGGAGCCGGAGACGGGTTAGCAGAGGGCACCGCCAGCTCAT
CCACCGATGGAGCACCAAGCGCCCCAACAGCAGTCGGCCTCGCCATCACC
AACACGGACGTTTTGAGCCACCTCCTAGACTTCGTGGCAGAGGACCAATA
TCTCTTCTTCGCCGCGGTCTCGCGAGCCTGGACGTCGGTGTGGAGGGCTG
CCCAACGCCCCGCGGCCACGAGGCAAGTCACGCCAGACACGTCCGAGTCT
CAGCTGCGAGAGAGTCTCGAGGCCGGCTTGCCTCTAGACAAGGCACAGCT
GTCGGCAGCCCTCGCACGCCACGGGCATCTAGAGCTGCTGCAGCTGGCCA
GGGAGCGCGGCTGCGCGTGGGGCGCGGTGACCTGTTCCGCCGCCGCCGGG
GCGGGACACCTGGAGCTCCTCCAGTGGTGCAAGTACAACGGGTGCCCATG
GGACGACCGCACGCTGACAGAGGCCGCGCGCGGCGACCACGCGGAGGTCC
TGGAGTGGGCGAGGATGTTCGGCTGCGAGGTGAGCATGGGCGTCCCCACC
GCGGCGGCGGGGGCGGGGAACCTGAACCTCCTCAAGTACTTGATCCAGGA
CGGGTTTCCGGTTTGTGCGGGGGCTTGCGGCGCGGCGGCTAAATCCGGCC
ACCTGGAAATCCTGGAGTACTTGGTCGAGAACGAGTGCCCTGTCGACGAG
ATGACGCTCTTTGGGGTCGTTCTCAACGGCCAGCTAGCTGTCGTGGAGTG
GGCGCTCGGGGAAGGGGTCGAGTTTAACGACCTCGGGGAGTACGCCTTCT
GCGACGCCGTGAAGAAGGGCCGATTGGCGGTGGTCCGTCTTCTGCATCGG
CACGGGTGTCCGTGGGACGAGGCGAGCTGTTTGGCGGCGGCGATCGAGGG
TGGTCACGAGGAAATCGCTAGCTGGATAAGGGAGAACCGATCGTAAGGCA
CCGCCAGCTCATCCACCGATGGAGCACCAAGCGCCCCAACAGCAGTCGGC
CTCGCCATCACCAACACGGACGTTTTGAGCCACCTCCTAGACTTCGTGGC
AGAGGACCAATATCTCTTCTTCGCCGCGGTCTCGCGAGCCTGGACGTCGG
TGTGGAGGGCTGCCCAACGCCCCGCGGCCACGAGGCAAGTCACGCCAGAC
ACGTCCGAGTCTCAGCTGCGAGAGAGTCTCGAGGCCGGCTTGCCTCTAGA
CAAGGCACAGCTGTCGGCAGCCCTCGCACGCCACGGGCATCTAGAGCTGC
TGCAGCTGGCCAGGGAGCGCGGCTGCGCGTGGGGCGCGGTGACCTGTTCC
GCCGCCGCCGGGGCGGGACACCTGGAGCTCCTCCAGTGGTGCAAGTACAA
CGGGTGCCCATGGGACGACCGCACGCTGACAGAGGCCGCGCGCGGCGACC
ACGCGGAGGTCCTGGAGTGGGCGAGGATGTTCGGCTGCGAGGTGAGCATG
GGCGTCCCCACCGCGGCGGCGGGGGCGGGGAACCTGAACCTCCTCAAGTA
CTTGATCCAGGACGGGTTTCCGGTTTGTGCGGGGGCTTGCGGCGCGGCGG
CTAAATCCGGCCACCTGGAAATCCTGGAGTACTTGGTCGAGAACGAGTGC
CCTGTCGACGAGATGACGCTCTTTGGGGTCGTTCTCAACGGCCAGCTAGC
TGTCGTGGAGTGGGCGCTCGGGGAAGGGGTCGAGTTTAACGACCTCGGGG
AGTACGCCTTCTGCGACGCCGTGAAGAAGGGCCGATTGGCGGTGGTCCGT
CTTCTGCATCGGCACGGGTGTCCGTGGGACGAGGCGAGCTGTTTGGCGGC
GGCGATCGAGGGTGGTCACGAGGAAATCGCTAGCTGGATAAGGGAGAACC
GATCGTAA
back to top