mRNA_C-linearis_contig102.802.1 (mRNA) Chordaria linearis ClinC8C monoicous

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: D8LRB9_ECTSI (Regulator of chromosome condensation, RCC1 Ankyrin ubiquitin interaction motif, zinc finger protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LRB9_ECTSI)

HSP 1 Score: 1374 bits (3556), Expect = 0.000e+0
Identity = 821/1324 (62.01%), Postives = 957/1324 (72.28%), Query Frame = 2

Query:  587 MTSIWDHVLDGNIAGLRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADVVRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHGSSGSNARLVPKRVESLKKFTVVGVAAGTEHTAVVTLSGNCYTFGRNNHGQLALAGVKETRSPRLVSGLSSGASRGAGGVGRYVLKVVSASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSSSRWQTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERGPQLVHI----SCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSHERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPHGASFTSVDPLSGAQVDDDDALTEETGEGAEGDLDSDGDEEESLGDVDSKIAGRDGLVSANADGGFEPLTLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGILVIGRESDRSCLLEASASGVASSLLCGARARAPTARTAAAKGASAVDE----LEAEEGLDRELQATALARAEMRSKTPSAKVAARAVRSLKKKIARIREWELAKERGKELSEQQVSKVSQKGRMEAELTSLDSQLERAKLAHAARCSRLAAGRPSETEASPLSK-----RVLFPDETGL-GDNHPMLDPDLQAG-----KTDRWTPQLDLSQE-TETNDKRSIMCEACSVECANASMYADHLRGKRHRARILRLEQQL----KAAQPDPKVPRTWATT-LVQDCSAVAAPC--AAAT----PTDAQLCGSGKGRRASCPEANWPSRSYRAHR---------------QALEEQQRSATVLMSDKGKNETADAAQAMSLLRTPAK--SQRSPSPQESAAATPTAVYSLVDFIATPGTAKPPRRAKGDKGAGKVWGEDVEAG-----RKSASSAE----RPLTTPKKSFQEILKEEAEAKKEREDYGGTGAWFI--SGKPRSTSFESIVQQQRREERIAQEEQLRQMEEEMLNVALEMSKHEAQPKLAPSHSRSKGSRKGRGAQIDSGVGKNKESSRPRKHPSTC---SQP----------------------QQSRHGRRRRVKRTAEVDEAGPSNT 4306
            M +IWDHVLD ++ G+ ++LEDG  VD RN LGET LHLAAR+GLE+MAT LIDAGASVH+ D+ESGWTPLHRSLYFGH+RLSLLLL+AGAL+DGGRDQ  R+ RQK                + +S  +  RR NDGLGD EGNSPLDVLSLELR+ LK ARESG+GGDVFSFGKADFTLGYDSF RAD++ PRR+E+LANLHVTRV+ASKHHSAA+T CG+L+TWGHGKSGRLGHG+E  CMLPT V+GLAHQ VT+V+ AE+HTAALT  G +YTWGRDRFGQLGHGS+GS+ R  PKRVE L+K TVV VAAGTEHTAV T SG+CYTFGRNNHGQL LAGVKET +PR+VS L+SGASRGAGGVGR+V+KV SASARST+V+TRG+DRGSGL  VNEV  WG G  +PSRV+FN    AS SSRWQTHDAR+DI+D+AAARCHN+ALDR+G VF+WGFGAD+LGL PRE+RT RGPQLV      +CGGNP+ +SASDQHSCVVTD GDLFTWGT   EGGALGH   RWQP+AKRVP LKKV  VAAAP+HTVVL  A CPSLPHGA+F +VD LS    +   +L EE GE A+ D+D DGD+E      DS   G  GL + N   GFEPLTLKQ CEAKLA+EVDLHNAGA+LAYADALDAPAL+ +CAE+V+ NLD +LV+G  S  SCLLEAS   V SSLLCGAR+R PTAR +A    S  D     L  EEGLD ELQATALARA+MR+KTPSAKVAARAVRSLKKKIAR++EWE  +E+GK+LS  Q+SKV Q GRMEAELTSLDSQLERAKLA AAR SRL AG    TEASP SK     R LF +E+G+ G + P LDPDL A      KT  WTPQLDLS + T+  +KR+I CEACS++CA+A+MYADHLRG+RHRA  LRLEQQL    K +QP+P+VPR+WA+    QD SA AA    AAA     PT+A+L    +GR+ASCPEA+ PS +++A R                ALE QQR+AT+LM   GK E A A Q MS LR+  +  SQR    Q+ A  T  AVYSLVDF+A     K  RR +  +    V     EA         +S AE     PL T +KSF EIL+EE   K ERE+YG   AWFI   GKPRSTSFE IVQQQRR   +A+EE+LRQMEEEML +ALEMSK EAQP   P+ SR  GSRKGR    +   G+ +ES  PRKHPS     SQP                       QS +GRRRR KR++ V+  G   T
Sbjct:    1 MANIWDHVLDASLDGVGSMLEDGVPVDSRNSLGETPLHLAARHGLEEMATVLIDAGASVHLKDWESGWTPLHRSLYFGHIRLSLLLLQAGALLDGGRDQPDRDYRQKHRARSRSESVSST--GKYRSGTSARRRINDGLGDFEGNSPLDVLSLELRNHLKIARESGVGGDVFSFGKADFTLGYDSFGRADIIPPRRVESLANLHVTRVAASKHHSAAVTVCGKLFTWGHGKSGRLGHGNEEVCMLPTPVDGLAHQAVTDVSVAETHTAALTREGELYTWGRDRFGQLGHGSAGSSGRFAPKRVEGLRKVTVVAVAAGTEHTAVATSSGHCYTFGRNNHGQLGLAGVKETCAPRVVSYLASGASRGAGGVGRHVIKV-SASARSTVVVTRGIDRGSGLQTVNEVHQWGHGSPVPSRVSFNSAKDASPSSRWQTHDARVDIVDVAAARCHNVALDRVGMVFTWGFGADHLGLDPRETRTARGPQLVAAMLPENCGGNPISVSASDQHSCVVTDTGDLFTWGTSGGEGGALGHGSNRWQPLAKRVPSLKKVARVAAAPDHTVVLLQASCPSLPHGAAFATVDALSNEHEEGGHSLIEEEGEDADSDID-DGDDERR----DSSSHGG-GLFTTNPARGFEPLTLKQCCEAKLAREVDLHNAGAMLAYADALDAPALLLYCAEYVKRNLDAVLVLGCASTTSCLLEASGELV-SSLLCGARSREPTARASAVAEPSVADGAGFGLHEEEGLDLELQATALARADMRAKTPSAKVAARAVRSLKKKIARVKEWERMREKGKDLSADQMSKVDQMGRMEAELTSLDSQLERAKLAQAARSSRLEAGSARATEASPSSKTPEVKRTLFRNESGMDGRDIPALDPDLDATSASSLKTGEWTPQLDLSHDDTKATEKRTINCEACSIKCASAAMYADHLRGRRHRATTLRLEQQLHKATKESQPEPQVPRSWASVPAAQDHSAAAAAALDAAANCNTVPTNAKLPHRSEGRQASCPEAS-PSHAHQARRPSNQLPRGKELSTMRHALEGQQRAATMLMGSAGKEEAATATQGMSFLRSTTRGDSQRQQLQQQDAPTT--AVYSLVDFMAPSHGKKSSRRTQASEKPKAVHAWKSEAAGPVEPNSPSSPAEAKEFEPLRT-RKSFHEILEEEEREKLEREEYG-KNAWFIPGKGKPRSTSFEGIVQQQRRXXMVAEEEKLRQMEEEMLGLALEMSKQEAQP--TPAQSRGSGSRKGR----EGRGGRKQESRAPRKHPSARGKGSQPLGGVETGAXXXXXXXXXXXDPRAQSGNGRRRRAKRSSAVENQGAEPT 1303          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: A0A4D9D2C8_9STRA (Uncharacterized protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D2C8_9STRA)

HSP 1 Score: 473 bits (1216), Expect = 2.820e-137
Identity = 367/1014 (36.19%), Postives = 523/1014 (51.58%), Query Frame = 2

Query:  593 SIWDHVLDGNIAGLRTLLEDGGG-------VDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMD-------------GGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADVVRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHGSSGSN---ARLVPKRVESLKKFTVVGVAAGTEHTAVVTLSGNCYTFGRNNHGQLALAGVKETRS--PRLVSGLSSGASRGAGGVGRYVLKVVSASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKD--------------GASSSSRWQTHD------------ARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERG-------PQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSHERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPHG-ASFTSV--DPLSGAQV-DDDDALTEETGEGAEGDLDSDGDEEESLGDVDSKIA------GRDGLVSANADGGFEPL-----TLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGILVIGRESDRSCLLEASASGVASSLLCGARARAPTARTAAAKGASAVDELEAEEGLDRELQATA---------LARAEMRSKTPSAKVAARAVRSLKKKIARIREWELAKERGKELSEQQVSKVSQKGRMEAELTSLDSQLERAKLAHAARC-SRLAAGRPSETEASPLSKRVLFPDETGLGDNHPM---LDPDLQAGKTDRWTPQLDLSQETETNDKRSIMCEACSVECANASMYADHLRGKRHRARILRLEQQLKAAQ 3376
             +W      ++A LRTL+ +GGG       VDER+  G T+LH A+  G E     L+  GA+ +  D ES WT LHR+LY GH+R++LLLL+AGA++              GG++   R ++                      +   ++ + DG+ D +G +PL +LS  LR  L+ A     GGDV +FGKADF LGY     ++V +PRR+EALA++ V  V+A++HHS  + + GR++TWGHG+ GRLGHG E   M P  V  L    V ++A AE+HT  +T  G V++WG DRFGQLG G S +    ARLVP+RVE+L++  V  V+AG  HT  VT  G  YT+G N  GQL   GV       PR+VS L     R  G   R  ++V SA    T+V+  G    +G    N+VF +G G  +P RV+F   +              G  +S    + D            ARI+++ ++A R HN+AL   G VF+WG GAD LGL    +  E G       P++VH       V +SAS  H+CVV++ GDL++WG+ D + G LGH H  WQPV KRV  LK+   VAA P HTVVL  A  P+LPH  A+  +V  + ++G +V ++ D   E     +    D+  + + +L ++D          G D + S+   G   P      +LK+LCE  +A +VD+ NA +VLA+A+ALDA  L  FC EFV+ NLDGILV GR +D   LLE +A  V +         AP++ +A    AS + E  +    D   +ATA         LAR E R + P+ + A R  R LKK++ ++ E    +  G  LS  Q  K+S++  +E E  +L   L R ++   AR       G+   TEAS L K    P    L   H +   L+PD Q G    +  +  L   T +  K    CEAC V CA  S++ DH++GKRH   + + + +  A Q
Sbjct:   10 DVWASAEADDVAALRTLMGEGGGNENLDMYVDERDDHGRTALHAASSKGAESAVRFLLSRGANPNKADRESHWTSLHRALYHGHLRIALLLLRAGAVLGDELTPWQAGHHLRGGKNNGSRNNQTTTRSFL--------------DKKESSQSRKDGV-DKDGLTPLSLLSWRLRKHLQEAVRENKGGDVVTFGKADFPLGYPLPNTSNVQKPRRVEALADVAVVAVAAARHHSVVLDKDGRVFTWGHGRGGRLGHGDENPQMFPKLVAALDGLRVVKIAAAENHTVCVTDQGLVFSWGSDRFGQLGVGGSSAGQGTARLVPRRVEALRRCMVADVSAGATHTVAVTREGEVYTWGGNKSGQLGYEGVGAIGDGVPRMVSLLYH---RANGKDPRRAIQV-SAGCSCTLVVVAG----AGPYGPNKVFQFGYGSHVPIRVDFRPAEKAQRETGKRKGRGEGRGNSGSTGSKDMFWAAPGGACGGARINVVQVSAGRHHNVALASTGLVFTWGLGADQLGLGVGGAGQEEGGVSHTTCPRVVHGMGQDRGVFVSASAGHTCVVSENGDLYSWGSTDGKQGILGHGHGSWQPVPKRVVGLKRGVKVAAGPEHTVVLLLASSPALPHPHAALRAVRSELVAGKKVCEEGDGEEEADSRSSSPFSDTRSEADGNLFEIDDVETFKPLSHGSDEMGSSTQIGHPVPAVQPNPSLKELCERTIAAQVDMRNAISVLAHAEALDAKDLAGFCVEFVQRNLDGILVAGRPADLDFLLEDAADDVNTLFQ-----DAPSSLSANTPHASQLKEQSSASAGD---EATAARPSPVAVMLARVE-RGQDPTLEEALRLERGLKKRMTQVVELARMEADGTTLSPDQTQKLSRRSILEKEAIALVPLLARLRMKDQARTVGGRVDGKGIATEASCLVKEQKGPSGGELIGEHLLVSGLNPD-QHGSVSAYEEKSFL---TPSPTKVVYSCEACHVTCATESIWQDHIKGKRHSKCLAKAKAEAAAKQ 987          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: A0A835ZA25_9STRA (Cilia- and flagella-associated protein 300 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZA25_9STRA)

HSP 1 Score: 431 bits (1108), Expect = 1.960e-122
Identity = 297/721 (41.19%), Postives = 398/721 (55.20%), Query Frame = 2

Query:  683 GETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADVVRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHGSSGSNARL------VPKRVESLKKFTVVGVAAGTEHTAVVTLSGNCYTFGRNNHGQLALAGVK--ETRSPRLVSGLSSGASRGAGGVGRYVLKVVSASARSTMVITRGVDRGSGL-PPVNEVFCWGLGLILPSRVNFNMK-----DGASSSSRWQTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGL--QPRESRTERGPQLVHIS----CGGN-PVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSHERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPH------------------GASFTSVDPLSGAQVDDDDALTEETGEGAEGDLDSDGDEEESLGDVDSK---------IAGRDGLVSANADGGFEP------LTLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGILVIGRESDRSCLLEA 2683
            GET LHLAA++G++D    L+ AGA   + D ++  TPLH SL  GHVR S  LL  GA  D G D+V   D+                 S     ++ +RR+   L D +G SPLD+LS +L   L  A  +G G +V+SFGKAD+ LG++    ADV   RR+ AL +++  R++AS+H S A+TR G LY+WGHGK GRLGHG E   +LP RVE LA + V +V  AE+H  A+T+ G V+TWG +RF QLGH SS             PKRVE+L++  VV  AAG  HTA VTL+GN +T+G N   QL    +    T +PRLV  + +GA    GG GR     V+A+A ST+VI  GV   SG  PPVNEV+ WG G   P RV F+++     DG  +      H + ++I  ++AA+ HN AL   G+VF+WGFGA+NLGL   P ESR+   PQLV       CGG  P ++SA+D H+ VVT  GDLFTWG  D     LG   +R+QPVA+RV  +K+  +VA A +HTVVL AA  P  PH                   A   S+  L  A    ++    +         D + +E E+   +D           +   DG   +N+  G         L+LKQLCE  LA+EVDL +   +LA+A AL+APAL  FCAE+ R NLD +LV+G  +    LL+A
Sbjct:   35 GETCLHLAAKHGVDDCLLTLLAAGADPLLKD-KAARTPLHLSLAHGHVRASTALLMYGA--DLG-DEVAMRDK-----------------SSKAGNSSSSRRKCQAL-DSQGRSPLDMLSPQLSEHLVQAAGTGFGAEVYSFGKADYQLGFEPPNAADVQYARRVGALKHMNAVRIAASRHASMAVTRGGDLYSWGHGKGGRLGHGDEAVQLLPRRVEALAGRIVVDVCAAENHAVAITAEGDVFTWGSNRFHQLGHSSSXXXXXXXXXXCATPKRVEALRRHRVVAAAAGACHTAAVTLAGNVWTWGSNKACQLGHPTLPAGHTSAPRLVGLVGAGAGA-VGGSGRRAF-AVAAAAHSTLVIAEGVGSSSGGGPPVNEVYQWGHGNANPMRVFFSLQGQAQDDGDDAGWLLPRH-SHVNITQVSAAKHHNAALSSAGQVFTWGFGAENLGLGKTPAESRS-CSPQLVTAMLPERCGGGIPTYVSATDMHTAVVTSNGDLFTWGAADAPA-TLGQGGDRFQPVARRVHGVKRALAVATAVDHTVVLLAASVPPPPHCADPAATQAPRRLGPLGRAALRASLPSLHAALYAAEETSNGDASPALSQHSDDEDEEHETAAGLDPHDDTLYSGGYLPVADGSDDSNSGAGSGDAAAGGVLSLKQLCEVILAREVDLKSVPYILAHAHALEAPALTAFCAEYARRNLDAVLVLGGRAAWDLLLDA 728          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: D0NM06_PHYIT (Regulator of chromosome condensation (RCC1)-like protein n=1 Tax=Phytophthora infestans (strain T30-4) TaxID=403677 RepID=D0NM06_PHYIT)

HSP 1 Score: 275 bits (703), Expect = 4.010e-75
Identity = 232/750 (30.93%), Postives = 333/750 (44.40%), Query Frame = 2

Query:  572 RPKLH--MTSIWDHVLDGNIA-GLRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHV--RLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADV-VRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHG--SSGSNARLVPKRVESLKKFTVVGVAAGTEHTAVVTLS-GNCYTFGRNNHGQLALAGVKE-------TRSPRLVSGLSSGASRGAGGVGRYVLKVV------------SASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSS-------SRW--QTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERG----------------PQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSH-ERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPHGASFTSVDPLSGAQVDDDDALTEETGEGAEGDLDSDGDEEESLGDVDSKIAGRDGLVSANADGGFEPLTLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGIL-VIGRE 2656
            +P +H  +  +W        A  L  LL+D    D+RN  GET+LH+AA +G ++    L+   A++   D+ESGWTPLHRSLY  H+    SLLLL+        + + G++  +K                                 D    SP+ +LS  L+   + ++ +  GG +++FGK D+ LGY     ADV + PR +E   N  + ++SASK+H+ A+   G  + WG GK GRLG G E  C+ PTR+E L    + +V   E+HT AL+ +G V++WG + FGQLGH   SS S +RL PKR+++ +   V  +AA   H+A +    G  YT+G N  GQL   G KE         +P+ V  L            R+ + VV            + S   T V+ R    G  L    +++ +G G   PSRVN      AS+S         W        +DI++++ A+ H+IAL   G VF+WG     L  QP  S  +R                 PQ V +   G    + AS  H  VVT  GDL TWG C ++G  LGH     WQP  KRV  +KK  +VAA   HT VL A   P       F  V                                        +KIA  D +            TL +L E K+A  VD+ N   V  +A+   A  L  +C E++R+N D +L  +GR+
Sbjct:    2 QPSIHANVLDLWACARSPEAAPALARLLQDTVPPDDRNNFGETALHVAAAHGNDEAVLLLLRYEANMLAADWESGWTPLHRSLYHQHLSSESSLLLLRHA------QTRFGKKFLRKLLHETR---------------------------DHSQQSPMQLLSTRLQHH-RNSQGACDGGLIYTFGKRDYQLGYH-LPNADVQITPRLVELPTNSPIVQLSASKYHTIALNAAGECFVWGFGKGGRLGTGSEFDCVEPTRLESLETTPMKKVVAGENHTMALSRTGQVFSWGSNSFGQLGHRGKSSSSQSRLTPKRIDAFRFHLVADIAASGCHSAAIDADDGAVYTWGSNRRGQL---GRKEGCGTDQADATPQCVDALRP----------RHPMCVVYGAYDFVRAEKLALSDWHTCVVLRCAHNGRSL---GQIWQFGYGSYRPSRVNLPSGRSASTSVAGAVMCDTWIPTCKQRAVDIVEVSCAQNHSIALSASGSVFTWGHNVPALSHQPSSSSQDRNASLFGDKVPPVPSPSAPQKVLLGTYGPVASVCASQDHCAVVTQQGDLVTWG-CGQQG-VLGHGRGNTWQPSPKRVAGVKKAVAVAAGHQHTAVLVAPVHPD------FKDV----------------------------------------AKIAAEDMVP-----------TLVELVERKIAAHVDVINCALVWQFAERYAARRLQSYCVEYMRYNWDAVLDAVGRD 641          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: A0A329RDJ0_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Phytophthora cactorum TaxID=29920 RepID=A0A329RDJ0_9STRA)

HSP 1 Score: 283 bits (724), Expect = 5.060e-75
Identity = 238/725 (32.83%), Postives = 324/725 (44.69%), Query Frame = 2

Query:  632 LRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADV-VRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHG--SSGSNARLVPKRVESLKKFTVVGVAAGTEHTAVVTLS-GNCYTFGRNNHGQLALAGVKE-------TRSPRLVSGLSSGASRGAGGVGRYVLKVVSASARS------------TMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNF-----NMKDGASSSSRW--QTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTER------------------GPQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSH-ERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPHGASFTSVDPLSGAQVDDDDALTEETGEGAEGDLDSDGDEEESLGDVDSKIAGRDGLVSANADGGFEPLTLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGIL-VIGRE 2656
            L  LL D    D+RN  GET+LH+AA +G +   T L+  GA +   D+ESGWTPLHRSLY  H+  SLLLL+        + + G++  +                         AR       D    SP+ +LS  L+   K   E+  GG V++FGK D+ LGY     AD+ V PR +E  A   + ++SASK+H+ A+   G  + WG GK GRLG G+E   + PTR+  L    + +VA  E+HT AL+ +G V++WG + FGQLGH   SS S +RL PKRV++ +   V  +AA   H+A +    G  YT+G N  GQL   G KE         +PR V  L +          R+ L VV     S            T V+ R    G  L    +V+ +G G   PSRVNF         GA     W        +DI++++ A+ H+IAL   G VF+WG     L  QP  S  +R                   PQ V ++  G    + AS  H  VVT  GDL TWG C ++G  LGH     WQP  KRV  +KK  +VAA   HT VL A   P       F SV                                        + IA +D + S           L +L E K+A  VD+ N   V  YA+   A  L  +C E++R N D +L  +GR+
Sbjct:   25 LARLLRDTVPPDDRNDFGETALHVAAAHGNDGAVTLLLRYGADLLTADWESGWTPLHRSLYHQHLSTSLLLLRHA------QTRFGKKFLRNFLHE--------------------AR-------DHSQQSPMQLLSTRLQHDHKPLEETCDGGLVYTFGKRDYQLGYH-LPNADMQVTPRLVELPAYSPIVQLSASKYHTIALNAAGECFVWGFGKGGRLGTGNEFDRVEPTRLASLETTPMKKVAAGENHTMALSRTGQVFSWGSNSFGQLGHPGKSSSSQSRLTPKRVDAFRFHVVTDIAASGCHSAAIDADDGAVYTWGGNRRGQL---GRKEGCGTDQADATPRSVDALRA----------RHPLCVVYGDYDSVRAERLALSDWHTCVVLRCAHNGRSL---GQVWQFGYGSYRPSRVNFPSAVSTSLAGAVMCDTWVPTCKQRGMDIVEVSCAQNHSIALSASGSVFTWGHNVQALSHQPSSSSQDRHASLPVSGNKASPMPSPSAPQKVSLANYGPVASVCASQDHCAVVTQQGDLVTWG-CGQQG-VLGHGRGNTWQPSPKRVAGVKKAVAVAAGHQHTAVLVAPVHPD------FNSV----------------------------------------ADIAAQDVVPS-----------LVELVERKIAAHVDVTNCALVWQYAERYAALRLQNYCIEYMRSNWDAVLDAVGRD 640          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: T0QQS6_SAPDV (Uncharacterized protein n=1 Tax=Saprolegnia diclina (strain VS20) TaxID=1156394 RepID=T0QQS6_SAPDV)

HSP 1 Score: 278 bits (711), Expect = 7.630e-74
Identity = 213/706 (30.17%), Postives = 313/706 (44.33%), Query Frame = 2

Query:  593 SIWDHVLDGNIAGLRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADVVRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHGSSGSNA--RLVPKRVESLKKFTVVGVAAGTEHTAVVTLSGNCYTFGRNNHGQLALAGVKET----RSPRLVSGLSSGASRGA--GGVGRYVLKVVSASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSSSRWQTHDAR----------IDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERGPQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSHERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPHGASFTSVDPLSGAQVDDDDALTEETGEGAEGDLDSDGDEEESLGDVDSKIAGRDGLVSANADGGFEPLTLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGILVIGRE 2656
            +IW   +  + A +  LL DG   +ERN LGET+LHLAA  G +D+ + L+  G S H+ D ESG+TPLHRSL   H+  SLLLL AGA +                                         ++  + D +G +P+D+LS +L +       S  GG++++FGK DF LGY      + V PRR++      + +V+A+++H+  ++  G  ++WG GK GRLG G E  C+ P  V     + V +VA  E+H+ ALT+ G +++WG + FGQLG     ++A  RL PKR+++ K   VV +AA + H+  +T SG+ +T+G N  GQL       T     SP+ V  L    +  A  G V       V+ S   T V+   V++ +      +V+ WG     PS+V   +    +  SR + H             I I DI+ A  H+I L   G V++WG G    G+Q         P + ++      V + A+ +                C    G+LGH    WQPV KRVP LK+ ++VAA P HTVVL A   P L                                                         +  AN     E  TL  LCE  LA ++ L        +ADA+ A ALV  C EF R N D +L + ++
Sbjct:   27 NIWAATVANDYATVERLLRDGVAANERNSLGETALHLAASRGYDDLLSLLLRHGGSPHLHDKESGYTPLHRSLLHDHINTSLLLLNAGAHL--------------------------------------TVEESSAVVDHDGLAPMDLLSTKLSATYPHQLASARGGEIYTFGKVDFQLGYHLPHGDEQVMPRRVKLPPQTDIVQVAAARYHTLVVSSSGDCFSWGFGKGGRLGTGTEFNCIHPVPVH-FPQRAVLKVAAGENHSLALTTGGQLFSWGSNSFGQLGFPLKLTSAASRLSPKRIDAFKGVVVVDIAASSVHSVALTESGHVFTWGSNKKGQLGRKDGFGTDQGFSSPKRVDALLPNHAHSAVLGDVTHVAAVKVAVSCNHTCVVLN-VEKETSAIVQGQVWQWGYNAYFPSQV---LLRHVAKESRLRQHHHNLWVPRCQQHAISIADISCAAQHSIGLSTAGNVYTWGHGP---GVQT--------PSINYVQLAHRAVSVCAA-KXXXXXXXXXXXXXXXXC----GSLGHEGRNWQPVPKRVPRLKRASAVAAGPQHTVVLVAPDRPQL---------------------------------------------------------VPPANE----ELPTLASLCEQSLAAQLSLETVVKTYNHADAIYATALVSVCNEFARRNFDAVLDMAKD 612          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: A0A833TJS9_PHYIN (Regulator of chromosome condensation (RCC1) repeat n=1 Tax=Phytophthora infestans TaxID=4787 RepID=A0A833TJS9_PHYIN)

HSP 1 Score: 280 bits (716), Expect = 8.200e-74
Identity = 232/748 (31.02%), Postives = 333/748 (44.52%), Query Frame = 2

Query:  572 RPKLH--MTSIWDHVLDGNIA-GLRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADV-VRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHG--SSGSNARLVPKRVESLKKFTVVGVAAGTEHTAVVTLS-GNCYTFGRNNHGQLALAGVKE-------TRSPRLVSGLSSGASRGAGGVGRYVLKVV------------SASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSS-------SRW--QTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERG----------------PQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSH-ERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSLPHGASFTSVDPLSGAQVDDDDALTEETGEGAEGDLDSDGDEEESLGDVDSKIAGRDGLVSANADGGFEPLTLKQLCEAKLAQEVDLHNAGAVLAYADALDAPALVRFCAEFVRWNLDGIL-VIGRE 2656
            +P +H  +  +W        A  L  LL+D    D+RN  GET+LH+AA +G ++    L+   A++   D+ESGWTPLHRSLY  H+  SLLLL+        + + G++  +K                                 D    SP+ +LS  L+   + ++ +  GG +++FGK D+ LGY     ADV + PR +E   N  + ++SASK+H+ A+   G  + WG GK GRLG G E  C+ PTR+E L    + +V   E+HT AL+ +G V++WG + FGQLGH   SS S +RL PKR+++ +   V  +AA   H+A +    G  YT+G N  GQL   G KE         +P+ V  L            R+ + VV            + S   T V+ R    G  L    +++ +G G   PSRVN      AS+S         W        +DI++++ A+ H+IAL   G VF+WG     L  QP  S  +R                 PQ V +   G    + AS  H  VVT  GDL TWG C ++G  LGH     WQP  KRV  +KK  +VAA   HT VL A   P       F  V                                        +KIA  D +            TL +L E K+A  VD+ N   V  +A+   A  L  +C E++R+N D +L  +GR+
Sbjct:    2 QPSIHANVLDLWACARSPEAAPALARLLQDTVPPDDRNNFGETALHVAAAHGNDEAVLLLLRYEANMLAADWESGWTPLHRSLYHQHLSTSLLLLRHA------QTRFGKKFLRKLLHETR---------------------------DHSQQSPMQLLSTRLQHH-RNSQGACDGGLIYTFGKRDYQLGYH-LPNADVQITPRLVELPTNSPIVQLSASKYHTIALNAAGECFVWGFGKGGRLGTGSEFDCVEPTRLESLETTPMKKVVAGENHTMALSRTGQVFSWGSNSFGQLGHRGKSSSSQSRLTPKRIDAFRFHLVADIAASGCHSAAIDADDGAVYTWGSNRRGQL---GRKEGCGTDQADATPQCVDALRP----------RHPMCVVYGAYDFVRAEKLALSDWHTCVVLRCAHNGRSL---GQIWQFGYGSYRPSRVNLPSGRSASTSVAGAVMCDTWIPTCKQRAVDIVEVSCAQNHSIALSASGSVFTWGHNVPALSHQPSSSSQDRNASLFGDKVPPVPSPSAPQKVLLGTYGPVASVCASQDHCAVVTQQGDLVTWG-CGQQG-VLGHGRGNTWQPSPKRVAGVKKAVAVAAGHQHTAVLVAPVHPD------FKDV----------------------------------------AKIAAEDMVP-----------TLVELVERKIAAHVDVINCALVWQFAERYAARRLQSYCVEYMRYNWDAVLDAVGRD 639          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: G4ZEP6_PHYSP (Uncharacterized protein (Fragment) n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4ZEP6_PHYSP)

HSP 1 Score: 270 bits (689), Expect = 2.150e-73
Identity = 212/613 (34.58%), Postives = 288/613 (46.98%), Query Frame = 2

Query:  632 LRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGL------GGDVFSFGKADFTLGYDSFERADV-VRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHG--SSGSNARLVPKRVESLKKFTVVGVAAGTEHTAVVTLS-GNCYTFGRNNHGQLALAGVKE-------TRSPRLVSGLSSGASRGAGGVGRYVLKVVSASARS------------TMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSSSR-------W--QTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESR---------TERGPQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSH-ERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCP---SLPHGASFTSVDPL 2317
            L  LL D    D+RN LGET+LH+AA  G ++    L+  GA +   D+ESGWTPLHRS+Y  H+     LL    L+   + + G++  +K                E +  A                SP+ +LS  L+ Q +  RE         GG V++FGK D+ LGY     ADV V PR +E  A   + ++SASK+H+ A+   G  + WG GK GRLG G+E   + PTR+  L    + +VA  E+HT AL+ SG V++WG + FGQLGH   SS S +RL PKRV++ +   +  +AA   H+A +    G  YT+G N  GQL   G KE         +PR V  L            R+ + VV     S            T V+ R    G  L    +V+ +G G   PSRVN      AS S         W        +D++D++ A+ H+IAL   G VF+WG     L  QP             +   PQ V ++  G    + AS  H  VVT  GDL TWG C ++G  LGH     WQP  KRV  +KK  +VAA   HT VL A   P   S PH A+  +V  L
Sbjct:   26 LARLLRDAAPPDDRNDLGETALHVAAARGNDEAVQLLLRHGADLLAADWESGWTPLHRSVYHQHLSSESTLL----LLRHAQTRFGKKFARKYLH-------------ETRDHAQ--------------QSPMQLLSTRLQHQRQPDREVATTTEACDGGLVYTFGKRDYQLGYH-LPNADVQVTPRLVELPAASPIVQLSASKYHTIALNAAGECFVWGFGKGGRLGTGNEFDRIEPTRLVSLETTPMKKVAAGENHTLALSRSGQVFSWGSNSFGQLGHPGKSSSSQSRLTPKRVDAFRFHEMKDIAASGCHSAAIDAEDGAVYTWGSNRRGQL---GRKEGCGTDQADATPRSVDALRP----------RHPMCVVYGDYDSVQAEKLALSDWHTCVVLRCSHNGRSL---GQVWQFGYGSYRPSRVNIPFDAAASGSGAGAIMCDAWVPTCKQRGMDVVDVSCAQNHSIALSASGAVFTWGHNVPALSHQPSAGTPGHNASTTLSPSAPQKVELTKAGPVASVCASQDHCAVVTREGDLVTWG-CGQQG-VLGHGRGNTWQPSPKRVSGVKKAVAVAAGHQHTAVLVAPVLPDFDSAPHVAAQDAVPSL 588          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: A0A1W0A931_9STRA (Regulator of chromosome condensation (RCC1) n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A931_9STRA)

HSP 1 Score: 273 bits (699), Expect = 1.690e-72
Identity = 189/575 (32.87%), Postives = 275/575 (47.83%), Query Frame = 2

Query:  587 MTSIWDHVLDGNIAGLRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADVVRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHGSSGSNA--RLVPKRVESLKKFTVVGVAAGTEHTAVVTLSGNCYTFGRNNHGQLALAGVKET-------RSPRLVSGLSSGASRGA--GGVGRYVLKVVSASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSSSR-----W--QTHDARIDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERGPQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSHERWQPVAKRVPLLKKVTSVAAAPNHTVVL 2257
            + +IW   +      +  LL DG  V+ERN LGET LHLAA  G +++   L+  GA  HV D+ESG+TPLHRSL  GH+  SLLLL  G+ +                                         +N    D +G SP+D+LS++L+SQ  +     LGG+V++FGK DF LGY      +   PRR++  A+  +  +SA+++H+  I+  G  ++WG GK GRLG G E  C+ P ++   AH+ V++VA  E+H+ ALTS G +++WG + +GQLG     ++A  RL+PKR+++LK  T V +AA + H+A +  SG+ YT+G N  GQL   G KE         +P+ V  L    +  A  G     V   V+ S   T V+     + + L P  +V+ WG     PS+V            +     W  + H   I IIDI+ A+ H+I L   G V++WG G                P     +    P+   A                         ++GH    WQPV KRVP LK+ T+V A P HT VL
Sbjct:   13 LDTIWAATIAKEYELVEALLRDGVNVNERNSLGETPLHLAAGRGYDEIIDLLLRYGADPHVHDWESGYTPLHRSLLQGHINASLLLLNGGSSL--------------------------------------TLEENLACVDDDGYSPMDLLSVKLQSQYPS--NPRLGGEVYTFGKVDFQLGYHLPHGDEQFIPRRVKFPADTDIIALSAARYHTLVISSKGECFSWGFGKGGRLGTGTEFNCIHPVQLS-FAHRVVSKVAAGENHSLALTSDGQLFSWGSNSYGQLGFPLKQTSAASRLIPKRIDALKGMTFVDIAASSVHSAAIATSGHVYTWGSNKKGQL---GRKEGFGSDQGYPTPKRVDALLPSQAHSAVLGDYTSVVATKVAVSCFHTCVVL-SAQKETSLYPQRQVWQWGYNAHFPSQVLLRHTSKEERLRQHHQNIWIPRCHQHNISIIDISCAQQHSIGLSSKGNVYTWGHG----------------PSCHATNINYVPLPHRAVSXXXXXXXXXXXXXXXXXXXXXXXSMGHEGRIWQPVPKRVPRLKRATAVVAGPQHTAVL 526          

BLAST of mRNA_C-linearis_contig102.802.1 vs. uniprot
Match: A0A067CHX3_SAPPC (Uncharacterized protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067CHX3_SAPPC)

HSP 1 Score: 273 bits (698), Expect = 2.880e-72
Identity = 188/585 (32.14%), Postives = 281/585 (48.03%), Query Frame = 2

Query:  593 SIWDHVLDGNIAGLRTLLEDGGGVDERNWLGETSLHLAARYGLEDMATALIDAGASVHVMDYESGWTPLHRSLYFGHVRLSLLLLKAGALMDGGRDQVGREDRQKXXXXXXXXXXXXXXPSECKSRATGARRQNDGLGDGEGNSPLDVLSLELRSQLKAARESGLGGDVFSFGKADFTLGYDSFERADVVRPRRIEALANLHVTRVSASKHHSAAITRCGRLYTWGHGKSGRLGHGHEGTCMLPTRVEGLAHQTVTEVATAESHTAALTSSGAVYTWGRDRFGQLGHGS--SGSNARLVPKRVESLKKFTVVGVAAGTEHTAVVTLSGNCYTFGRNNHGQLALAGVKET-------RSPRLVSGLSSGASRGAGGVGRYVL---KVVSASARSTMVITRGVDRGSGLPPVNEVFCWGLGLILPSRVNFNMKDGASSSSRWQTHDAR----------IDIIDIAAARCHNIALDRIGRVFSWGFGADNLGLQPRESRTERGPQLVHISCGGNPVHISASDQHSCVVTDAGDLFTWGTCDEEGGALGHSHERWQPVAKRVPLLKKVTSVAAAPNHTVVLCAAFCPSL 2281
            +IW   +  + A +  LL DG   +ERN LGET+LHLAA  G +D+ + L+  G S H+ D ESG+TPLHRSL + H+  SLLLL AGA +                                         ++  + D +G +P+D+LS +L +       S  GG++++FGK DF LGY      + V PRR++      + +++A+++H+  ++  G  ++WG GK GRLG G E  C+ P  V     +TV +VA  E+H+ ALT+ G +++WG + FGQLG     + +++RL PKR+++ K   VV +AA + H+  +T SG+ +++G N  GQL   G KE         SP+ V  L    +R      R +L    VV+ S   T ++       S      +V+ WG     PS+V   +    S  SR + H             I I+DI+ A  H+I L   G V++WG G    G+Q         P + ++      V +                     C    G+LGH    WQPV KRVP LK+ ++VAA P HTVVL A   P L
Sbjct:   17 NIWAATIANDYATVERLLRDGVAANERNSLGETALHLAASRGYDDLLSLLLRHGGSPHMHDKESGYTPLHRSLLYDHINTSLLLLNAGAHL--------------------------------------TVEESSAVVDHDGLAPMDLLSAKLSATYPHQMASTRGGEIYTFGKVDFQLGYHLPHGDEQVMPRRVKLPPKTDIVQIAAARYHTLVVSSSGECFSWGFGKGGRLGTGTEFNCIHPVPVP-FPQRTVVKVAAGENHSLALTTGGQIFSWGSNSFGQLGFPLKLTSASSRLSPKRIDAFKGVVVVDIAASSVHSVALTESGHVFSWGSNKKGQL---GRKEGFGTDQGFPSPKRVDALMR--TRRCSATSRTLLPSRSVVAVSCNHTCIVLNVEKETSAAVVQGQVWQWGYNAYFPSQV---LLRHVSKESRLRQHHRNLWVPRCQQHAISIVDISCAPQHSIGLSASGNVYTWGHGP---GVQS--------PSINYVPLAHRAVSVCXXXXXXXXXXXXXXXXXX-XC----GSLGHEGRNWQPVPKRVPRLKRASAVAAGPQHTVVLVAPDRPPL 538