mRNA_C-linearis_contig10.562.1 (mRNA) Chordaria linearis ClinC8C monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-linearis_contig10.562.1
Unique NamemRNA_C-linearis_contig10.562.1
TypemRNA
OrganismChordaria linearis ClinC8C monoicous (Chordaria linearis ClinC8C monoicous)
Homology
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BLAST of mRNA_C-linearis_contig10.562.1 vs. uniprot
Match: D7FQH0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQH0_ECTSI)

HSP 1 Score: 153 bits (386), Expect = 1.240e-36
Identity = 71/90 (78.89%), Postives = 84/90 (93.33%), Query Frame = 1 
Query:  499 VDQKDDDRYVRLLDVDSDAVWRAVLDACDRVGSYRVVGWCKRRWLMDVLFERPRDAAAAVDDIDHLRLASARSGRLIKTLIKPADFEGGF 768
            +DQ+DDDRYVR+LD+DSD +++AVLDACDRVGS+RVVGWCKRRWLMD+LFE  RDAAAA+DDIDHLRLAS+RS R IKTL+KPA F+GGF
Sbjct:   53 LDQRDDDRYVRILDIDSDPLYQAVLDACDRVGSFRVVGWCKRRWLMDILFESRRDAAAALDDIDHLRLASSRSRRPIKTLLKPARFDGGF 142
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BLAST of mRNA_C-linearis_contig10.562.1 vs. uniprot
Match: A0A6H5K1S2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1S2_9PHAE)

HSP 1 Score: 154 bits (388), Expect = 4.190e-36
Identity = 75/106 (70.75%), Postives = 89/106 (83.96%), Query Frame = 1 
Query:  502 DQKDDDRYVRLLDVDSDAVWRAVLDACDRVGSYRVVGWCKRRWLMDVLFERPRDAAAAVDDIDHLRLASARSGRLIKTLIKPADFEGGFARDDLSPTATAGTTAAA 819
            DQ+DDDRYVR+LD+DSD +++AVLDACDRVGS+RVVGWCKRRWLMD+LFE  RDAAAA+DDIDHLRLAS+RS R IKTL+KPA F GGF+ +    TA    + AA
Sbjct: 1086 DQRDDDRYVRILDIDSDPLYQAVLDACDRVGSFRVVGWCKRRWLMDILFESRRDAAAALDDIDHLRLASSRSRRPIKTLLKPARFNGGFSNNTSQATAAPPVSVAA 1191
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BLAST of mRNA_C-linearis_contig10.562.1 vs. uniprot
Match: A0A836CEP5_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CEP5_9STRA)

HSP 1 Score: 62.4 bits (150), Expect = 1.860e-6
Identity = 30/79 (37.97%), Postives = 49/79 (62.03%), Query Frame = 1 
Query:  523 YVRLLDVDSDAVWRAVLDACDRVGSYRVVGWCKRRWLMDVLFERPRDAAAAVDDIDHLRLASARSGRLIKTLIKPADFE 759
            Y+R+LD++     + ++  C R+GS+  +G  +++ +MDV FE PR+A  A++D+D L L S   GR   TL+K  D E
Sbjct: 1065 YLRVLDLE-----KRIMGVCKRIGSFNFIGRDRKKHVMDVRFESPREALVALEDVDALGLVSCIKGRPCNTLLKARDCE 1138
The following BLAST results are available for this feature:
BLAST of mRNA_C-linearis_contig10.562.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7FQH0_ECTSI1.240e-3678.89Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5K1S2_9PHAE4.190e-3670.75Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A836CEP5_9STRA1.860e-637.97Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-linearis_contig10contigC-linearis_contig10:2413228..2416595 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Chordaria linearis ClinC8C monoicous2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons5
Model size1511
Cds size1509
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-linearis_contig10.562.1prot_C-linearis_contig10.562.1Chordaria linearis ClinC8C monoicouspolypeptideC-linearis_contig10 2413228..2416594 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927323.5731387-CDS-C-linearis_contig10:2413227..24133181622927323.5731387-CDS-C-linearis_contig10:2413227..2413318Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2413228..2413318 +
1690879626.3498867-CDS-C-linearis_contig10:2413227..24133181690879626.3498867-CDS-C-linearis_contig10:2413227..2413318Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2413228..2413318 +
1622927323.6357238-CDS-C-linearis_contig10:2413691..24141071622927323.6357238-CDS-C-linearis_contig10:2413691..2414107Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2413692..2414107 +
1690879626.3597002-CDS-C-linearis_contig10:2413691..24141071690879626.3597002-CDS-C-linearis_contig10:2413691..2414107Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2413692..2414107 +
1622927323.6458116-CDS-C-linearis_contig10:2414490..24146421622927323.6458116-CDS-C-linearis_contig10:2414490..2414642Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2414491..2414642 +
1690879626.368447-CDS-C-linearis_contig10:2414490..24146421690879626.368447-CDS-C-linearis_contig10:2414490..2414642Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2414491..2414642 +
1622927323.6550322-CDS-C-linearis_contig10:2415219..24152921622927323.6550322-CDS-C-linearis_contig10:2415219..2415292Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2415220..2415292 +
1690879626.3766792-CDS-C-linearis_contig10:2415219..24152921690879626.3766792-CDS-C-linearis_contig10:2415219..2415292Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2415220..2415292 +
1622927323.6665382-CDS-C-linearis_contig10:2415817..24165941622927323.6665382-CDS-C-linearis_contig10:2415817..2416594Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2415818..2416594 +
1690879626.388105-CDS-C-linearis_contig10:2415817..24165941690879626.388105-CDS-C-linearis_contig10:2415817..2416594Chordaria linearis ClinC8C monoicousCDSC-linearis_contig10 2415818..2416594 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-linearis_contig10.562.1

>prot_C-linearis_contig10.562.1 ID=prot_C-linearis_contig10.562.1|Name=mRNA_C-linearis_contig10.562.1|organism=Chordaria linearis ClinC8C monoicous|type=polypeptide|length=503bp
QRQQQPLGTGPTSAASVGDVSAAAAPSAAEGSEMANECRDSTGGGDRDGQ
GAVAAAAAAAATRAPAAQPEALYPQPAAGQSASGARGGGGGGRGGSVEEL
ELSVRQYHEYPYPESVGSGSGRGRHSPVGSDQGSSFSARSVGGREETAET
RAQMLDPRRVAMSRVPVDQKDDDRYVRLLDVDSDAVWRAVLDACDRVGSY
RVVGWCKRRWLMDVLFERPRDAAAAVDDIDHLRLASARSGRLIKTLIKPA
DFEGGFARDDLSPTATAGTTAAAAALPAAATGPAAGAGTTAATVGAPAPG
ILTPDLSSEQSVPAVATPVPRMWSPPPPRHVTASPLGGSGGGQEGGAAAV
AAAAAELAAQVATASQVPDNAFSSTVAVSSGSGSGGGGAAGFHQQRGFGP
VPAPQHQKPAVASWAGGAGGDGGAFGAVEGQRNPLLDAAAATAASSGAGA
RGDGAARPPVGVSQPYPPVDASAVAGWGIAGRASGGGGGGSGGFLTGLGS
LQQ
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mRNA from alignment at C-linearis_contig10:2413228..2416595+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-linearis_contig10.562.1 ID=mRNA_C-linearis_contig10.562.1|Name=mRNA_C-linearis_contig10.562.1|organism=Chordaria linearis ClinC8C monoicous|type=mRNA|length=3368bp|location=Sequence derived from alignment at C-linearis_contig10:2413228..2416595+ (Chordaria linearis ClinC8C monoicous)
CAACGACAACAACAGCCGCTGGGAACCGGTCCCACCAGTGCCGCCAGCGT
CGGCGACGTTTCTGCGGCGGCCGCCCCATCGGCGGCGGAAGGTAAGGTGG
TTCAGCCTTGCCTCTTTGTTCTTGCAAGCGTATGGCATGTCTTTTCGAGT
TGGCTTTTTCTCTCCACATCGAAGCACACGCGTGTTTCGTGCGTGTCAAC
AAGTGTTTGATCCACTGCGCCTTTTTTTTGCCTTTTGTTTTCTTCGACTG
CCGTACCTCGTCGCCCAATCAACGAGCGCTTCGCATTCTTCTTCTTCGCT
TTCACGAAGTGTATTTTTTCGGTACAGCGGCATCGAGAAAACCGCAAAAG
AAACACGCCTCCTTTACCCTTCTTATTTTTCTCTCGTTACACTCTGCGCG
GTAAACATGGAAGATCTTGAGAAAACACGTCCCGTTTTTTTTTGTTCCCT
CTGCCGCGTCGCAGGCTCGGAGATGGCCAACGAGTGCCGTGACAGCACCG
GCGGCGGGGACAGAGACGGCCAGGGTGCCGTCGCCGCCGCCGCCGCCGCC
GCCGCTACTCGTGCTCCGGCCGCACAACCAGAGGCGTTGTACCCGCAACC
AGCAGCCGGGCAGAGCGCGTCGGGAGCACGGGGAGGAGGCGGAGGAGGAA
GAGGAGGGTCAGTGGAGGAGCTGGAGCTGTCGGTGCGGCAGTACCACGAG
TACCCCTACCCTGAGAGTGTCGGGAGCGGGAGCGGGCGGGGGCGACATAG
CCCCGTCGGGAGCGACCAGGGGAGCTCGTTCAGCGCGAGGTCGGTCGGGG
GCAGGGAGGAAACGGCGGAGACGAGAGCGCAAATGCTAGACCCACGGCGC
GTGGCGATGTCCAGGGTGCCGGTCGACCAGGTTTGTGGGGGTGGTTTGGG
TGCGGGGGGTAGGGGGTGCCGTGGGGGTGCGGGGTCGTTTTTGGCATGCC
CTTTTCCTCGTCGTTGAAGTTTTTGTTGAAAGTCCGCTTTGCCCTTGGTA
CCCACCCCTGCTAGACGCGCTGCCGCAACTACTGCTTGTGGTGGCCGTAC
CACCCAGACTTTGCTTTTTGGATGGGGTAGAGGGGCAATCAGTTCTCGAG
AGCGTTGCCGTAGGGTATTCAAACCGCAGACTTTAGCGTGTGCTTTGTGG
TAGTCCACGAAGGCCAAGCAAGGCCAAGCCGAAGGAGCGCCGCCCGGTTT
TAGGCCACCCACTCCCCTATCCTGTTGCTCTCCTGTCCTGTTTGTGTATG
TCCCGTGATCTAGAAGGACGACGACCGTTACGTCCGGCTGTTGGACGTAG
ACTCAGACGCGGTTTGGCGTGCCGTGCTCGACGCCTGCGACCGCGTGGGG
TCTTATCGGGTGGTGGGGTGGTGCAAGCGGCGGTGGCTCATGGACGTGCT
CTTCGAACGGCCGAGGTACGTGCGTACGTACGTGCTTTCCGAAAAATGTA
TTTTTGGACGCGGCGCGGTGCGGGGGGGAGTCAGTCGACAGGGTTGGGAC
TTTGGTGTGATGCTTGCGGGAAAGTGAATTTCATGGGGTGTCTTTCTCAG
CGTGCCGGAGACGGGAACAAGCAAAAGCACAAGAGAACAAAGAAACAACA
AAAACACCAACAACAAAAACACCAACAACAAAAACACCAACACCACCACC
AACAACAACAACGACAACAACGACAACAACAAAAACACCAACACCACCAC
CAACAACAACAACAACAACAACAACGCCGTTCTGTCTTGGATAGACCCGC
AGCTGCTGCTGTCCGGCCGGGCGGTGCTCTCGTGAGGGAAGACGTCGCTT
AGTTTTCTGCCCCTCTCCACTAGCTTAGCAGAAGCCTGATGATCCTCCAC
CGGACCTTCCGGACGCCGTATTAATCACCCAGCTGCGCAAAAACCAAAAC
GGTACTTACCGTCCGGCAGTCCTGCCCACTCATCTTGGTCTACTACTACT
GTATGCGTGTGTGTGTGTGTTTGTGTGTGTGTGTGTGTGCAGGGATGCCG
CCGCTGCCGTGGACGACATTGATCACTTGCGCCTGGCCAGCGCCCGATCC
GGCAGGCTGATCAAGGTAAGAGTTAAGGCACAAGAAGGCACAGGGCGCAG
GCACACGGCCTCTCCCGGGAGAATGTTTTTGGGGCCAACGGCCGAAGGTA
TCGGGAGCAACACATGTGTGGTCATACGAACAGGCAGCAGTGCCGTGCTT
CGTACATGTCTTGTGTAGAAGGATTGGATAATTAATGGACCCCAAGAAGG
TAAAAGTCCCCCGTAGAGCGCGTAAAGCTTGCTTGGCCTATGAAGGGTTT
TTCGTTTTTCACTTTTCACGTGTGAATGTTTTTTCTTCCGATTTTTGATT
TTTCGCCTGCTTGATCAAGCAAAGCCTTGCGTATGGCCGTTGGTTTCCCT
GCCTGCGAATCGCCCCGGTGACATCGCCTACGCTACTTGTGGGCCGTTCT
GCCTAGCCCTGCCCACACCTGCAGCCTACAGTAGTACCGCGCGTCCTTCC
CGCCTTGCCCTTGGTTGAAACTGTTTTTTATTCCGACAAGACCCGCCATC
CGTAACCACTACCCCCCCCCCCTTCTCCCCTCCCCGACAGACCCTCATCA
AACCCGCCGACTTCGAAGGAGGCTTCGCGAGGGACGACTTATCCCCGACG
GCGACAGCGGGGACAACAGCGGCGGCGGCGGCGTTGCCGGCCGCAGCAAC
GGGTCCGGCAGCGGGAGCGGGGACCACCGCGGCGACGGTGGGGGCCCCGG
CGCCGGGGATCCTGACCCCGGACCTGTCGTCGGAGCAGAGCGTCCCCGCG
GTGGCGACGCCGGTGCCGAGGATGTGGTCGCCGCCGCCCCCGCGCCACGT
GACGGCCTCCCCGCTCGGCGGCAGCGGTGGCGGCCAGGAAGGGGGCGCCG
CGGCAGTCGCGGCGGCGGCGGCCGAGCTGGCGGCGCAGGTGGCCACCGCC
AGCCAGGTCCCGGACAACGCGTTCTCAAGCACGGTGGCGGTTAGTAGTGG
CAGCGGTAGTGGTGGTGGCGGTGCTGCGGGTTTCCACCAGCAGAGGGGGT
TCGGACCAGTACCGGCCCCCCAGCACCAGAAGCCTGCGGTTGCGAGCTGG
GCCGGCGGCGCGGGCGGTGACGGCGGCGCTTTTGGGGCTGTCGAGGGTCA
GCGAAACCCTCTTCTGGATGCCGCTGCTGCTACTGCGGCGTCGTCCGGGG
CCGGCGCGCGCGGCGACGGCGCTGCTCGCCCCCCCGTGGGAGTGAGCCAG
CCTTATCCTCCCGTGGATGCGTCCGCGGTCGCAGGCTGGGGGATCGCGGG
ACGCGCGAGCGGCGGTGGTGGCGGCGGCAGTGGCGGGTTTTTGACGGGGC
TTGGCTCGTTGCAGCAGC
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Coding sequence (CDS) from alignment at C-linearis_contig10:2413228..2416595+

>mRNA_C-linearis_contig10.562.1 ID=mRNA_C-linearis_contig10.562.1|Name=mRNA_C-linearis_contig10.562.1|organism=Chordaria linearis ClinC8C monoicous|type=CDS|length=3018bp|location=Sequence derived from alignment at C-linearis_contig10:2413228..2416595+ (Chordaria linearis ClinC8C monoicous)
CAACGACAACAACAGCCGCTGGGAACCGGTCCCACCAGTGCCGCCAGCGT
CGGCGACGTTTCTGCGGCGGCCGCCCCATCGGCGGCGGAAGCAACGACAA
CAACAGCCGCTGGGAACCGGTCCCACCAGTGCCGCCAGCGTCGGCGACGT
TTCTGCGGCGGCCGCCCCATCGGCGGCGGAAGGCTCGGAGATGGCCAACG
AGTGCCGTGACAGCACCGGCGGCGGGGACAGAGACGGCCAGGGTGCCGTC
GCCGCCGCCGCCGCCGCCGCCGCTACTCGTGCTCCGGCCGCACAACCAGA
GGCGTTGTACCCGCAACCAGCAGCCGGGCAGAGCGCGTCGGGAGCACGGG
GAGGAGGCGGAGGAGGAAGAGGAGGGTCAGTGGAGGAGCTGGAGCTGTCG
GTGCGGCAGTACCACGAGTACCCCTACCCTGAGAGTGTCGGGAGCGGGAG
CGGGCGGGGGCGACATAGCCCCGTCGGGAGCGACCAGGGGAGCTCGTTCA
GCGCGAGGTCGGTCGGGGGCAGGGAGGAAACGGCGGAGACGAGAGCGCAA
ATGCTAGACCCACGGCGCGTGGCGATGTCCAGGGTGCCGGTCGACCAGGC
TCGGAGATGGCCAACGAGTGCCGTGACAGCACCGGCGGCGGGGACAGAGA
CGGCCAGGGTGCCGTCGCCGCCGCCGCCGCCGCCGCCGCTACTCGTGCTC
CGGCCGCACAACCAGAGGCGTTGTACCCGCAACCAGCAGCCGGGCAGAGC
GCGTCGGGAGCACGGGGAGGAGGCGGAGGAGGAAGAGGAGGGTCAGTGGA
GGAGCTGGAGCTGTCGGTGCGGCAGTACCACGAGTACCCCTACCCTGAGA
GTGTCGGGAGCGGGAGCGGGCGGGGGCGACATAGCCCCGTCGGGAGCGAC
CAGGGGAGCTCGTTCAGCGCGAGGTCGGTCGGGGGCAGGGAGGAAACGGC
GGAGACGAGAGCGCAAATGCTAGACCCACGGCGCGTGGCGATGTCCAGGG
TGCCGGTCGACCAGAAGGACGACGACCGTTACGTCCGGCTGTTGGACGTA
GACTCAGACGCGGTTTGGCGTGCCGTGCTCGACGCCTGCGACCGCGTGGG
GTCTTATCGGGTGGTGGGGTGGTGCAAGCGGCGGTGGCTCATGGACGTGC
TCTTCGAACGGCCGAGAAGGACGACGACCGTTACGTCCGGCTGTTGGACG
TAGACTCAGACGCGGTTTGGCGTGCCGTGCTCGACGCCTGCGACCGCGTG
GGGTCTTATCGGGTGGTGGGGTGGTGCAAGCGGCGGTGGCTCATGGACGT
GCTCTTCGAACGGCCGAGGGATGCCGCCGCTGCCGTGGACGACATTGATC
ACTTGCGCCTGGCCAGCGCCCGATCCGGCAGGCTGATCAAGGGATGCCGC
CGCTGCCGTGGACGACATTGATCACTTGCGCCTGGCCAGCGCCCGATCCG
GCAGGCTGATCAAGACCCTCATCAAACCCGCCGACTTCGAAGGAGGCTTC
GCGAGGGACGACTTATCCCCGACGGCGACAGCGGGGACAACAGCGGCGGC
GGCGGCGTTGCCGGCCGCAGCAACGGGTCCGGCAGCGGGAGCGGGGACCA
CCGCGGCGACGGTGGGGGCCCCGGCGCCGGGGATCCTGACCCCGGACCTG
TCGTCGGAGCAGAGCGTCCCCGCGGTGGCGACGCCGGTGCCGAGGATGTG
GTCGCCGCCGCCCCCGCGCCACGTGACGGCCTCCCCGCTCGGCGGCAGCG
GTGGCGGCCAGGAAGGGGGCGCCGCGGCAGTCGCGGCGGCGGCGGCCGAG
CTGGCGGCGCAGGTGGCCACCGCCAGCCAGGTCCCGGACAACGCGTTCTC
AAGCACGGTGGCGGTTAGTAGTGGCAGCGGTAGTGGTGGTGGCGGTGCTG
CGGGTTTCCACCAGCAGAGGGGGTTCGGACCAGTACCGGCCCCCCAGCAC
CAGAAGCCTGCGGTTGCGAGCTGGGCCGGCGGCGCGGGCGGTGACGGCGG
CGCTTTTGGGGCTGTCGAGGGTCAGCGAAACCCTCTTCTGGATGCCGCTG
CTGCTACTGCGGCGTCGTCCGGGGCCGGCGCGCGCGGCGACGGCGCTGCT
CGCCCCCCCGTGGGAGTGAGCCAGCCTTATCCTCCCGTGGATGCGTCCGC
GGTCGCAGGCTGGGGGATCGCGGGACGCGCGAGCGGCGGTGGTGGCGGCG
GCAGTGGCGGGTTTTTGACGGGGCTTGGCTCGTTGCAGCAGACCCTCATC
AAACCCGCCGACTTCGAAGGAGGCTTCGCGAGGGACGACTTATCCCCGAC
GGCGACAGCGGGGACAACAGCGGCGGCGGCGGCGTTGCCGGCCGCAGCAA
CGGGTCCGGCAGCGGGAGCGGGGACCACCGCGGCGACGGTGGGGGCCCCG
GCGCCGGGGATCCTGACCCCGGACCTGTCGTCGGAGCAGAGCGTCCCCGC
GGTGGCGACGCCGGTGCCGAGGATGTGGTCGCCGCCGCCCCCGCGCCACG
TGACGGCCTCCCCGCTCGGCGGCAGCGGTGGCGGCCAGGAAGGGGGCGCC
GCGGCAGTCGCGGCGGCGGCGGCCGAGCTGGCGGCGCAGGTGGCCACCGC
CAGCCAGGTCCCGGACAACGCGTTCTCAAGCACGGTGGCGGTTAGTAGTG
GCAGCGGTAGTGGTGGTGGCGGTGCTGCGGGTTTCCACCAGCAGAGGGGG
TTCGGACCAGTACCGGCCCCCCAGCACCAGAAGCCTGCGGTTGCGAGCTG
GGCCGGCGGCGCGGGCGGTGACGGCGGCGCTTTTGGGGCTGTCGAGGGTC
AGCGAAACCCTCTTCTGGATGCCGCTGCTGCTACTGCGGCGTCGTCCGGG
GCCGGCGCGCGCGGCGACGGCGCTGCTCGCCCCCCCGTGGGAGTGAGCCA
GCCTTATCCTCCCGTGGATGCGTCCGCGGTCGCAGGCTGGGGGATCGCGG
GACGCGCGAGCGGCGGTGGTGGCGGCGGCAGTGGCGGGTTTTTGACGGGG
CTTGGCTCGTTGCAGCAG
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