mRNA_A-nodosum_M_contig1167.22.2 (mRNA) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: D7FS01_ECTSI (Beta-galactosidase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FS01_ECTSI)

HSP 1 Score: 1322 bits (3421), Expect = 0.000e+0
Identity = 751/1503 (49.97%), Postives = 871/1503 (57.95%), Query Frame = 2

Query: 1205 LPELLTTSPPCWQNPQVLGFNKLRARTTVGAFSSVEQARANVELPTASENVLSLDGTWRFALHASPEKAIDSGFFRKGFQEDAFAA-----GKG--------DTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANS-------EKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSS----STDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRG-PVAQASCSTAGLLVGVYLKVSRPKLWTAERPHLYTLVVSTHTVGPDGKVDEE-SPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPKPSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSA----------------QRSATDVLCPMYARVEECVRLVKADAKR-----------GGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGW---------GRGEVATVRVEFEAPAAWTADA-LELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDG-----EQSLRGLSLQFHRAPTDNDRGGYEGQWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVESETPLGTSSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVG-----------EEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLV 5476
            LP LLT +P CW+NP VLGF KL+ARTT+GAFSSV+QARAN ELP AS NVLSLDGTWRFALH+ PE A+ S FF + F+E A        G G         +GGVRGADG W+DTPVPSCWQ+QGYDVPIYTN +YPFPV PP VPA NPTGCYRL FS+PD WG             E+R++ILHFAGVD AFFAWVNG L GFSKDSRLPAEF++T+ + +GSS+KNVLSVM +RWSDASYLEDQDHWWLSGIHRSVRLV+LPK C+LSDFSW AV S+    + D+ EARL++RCL++R     T    P   A  V +RA LFEEG+M                                     RG PVA+ASC+T+G+ VG+YLKV RP+LWTAE+PHLYTLVVS H VGP  +VDEE +PLQCESSR+     E+SGGQL+VNGR ITVAGANRHEHDD GGKVVPLESMVRD L+MKR NFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGM+P PSRL++DPDW+ A +ERL+ MVERDKNHPSII+WSLGNEAG+G  HY M+ W R RD SR IMYEPAM EGS                   SATDV+CPMYARVE+CVR +  D ++               RPLILCEYSHAMGNSNGNL KYW+LFRS+P+CQGGFVWDWVDQGL+ T+P       G        VE WGYGGDF EP+ND NFC+NGVVWPDR PHPAMEEFK+LMQPFH +++P G+       S+                                                ++L++ NRYD  D  +LWD+L F W +E DGVVVA                                                G G V    + FE+  + T     E WLTVTGRLR    WA AG+ VGH QLELP +                                                PE                  + LQLLG   G G  D +E ++     DG     E S R L LQF+RAPTDNDRGGY G+WD AGLL PALGPF  +  W RR+ DGAV+VTTEFKLRPR PKP LC++  KV                              SFVR+ AA WRLG A   N  G G G         VRVWLP VRL++THD+A GMS+APE                           D +GVG           E  E FDAGE  C++ Y + P G L  E DV+MPEHWPV+PRVG+R LLP +FSSIRWFGRG HENYPDRKAS  +  HAS+VA+QLTPYIRPGECG K+DVRWLEL                                                                  PPRP   +TAVSLDH +MGVGGDDSWS+ VH+E+LV
Sbjct:   89 LPALLTATPRCWENPLVLGFRKLKARTTLGAFSSVQQARANPELPEASNNVLSLDGTWRFALHSCPEHALASRFFSRKFREAAAGGLLERGGSGVXXXXXXXXSGGVRGADGRWRDTPVPSCWQMQGYDVPIYTNIQYPFPVNPPTVPADNPTGCYRLEFSLPDAWGTSGXXXXXXRTAVEQRRVILHFAGVDSAFFAWVNGHLVGFSKDSRLPAEFEVTERVLYGSSAKNVLSVMVLRWSDASYLEDQDHWWLSGIHRSVRLVSLPKFCSLSDFSWHAVFSAPPHTNEDIDEARLSIRCLVDRPPEPTT----PPGGAPAVQIRAHLFEEGVM--AAPSTAAVLPSRGPEEAGRDGGGSRMGVQGEGGSGRGAPVARASCTTSGMYVGLYLKVPRPQLWTAEQPHLYTLVVSMHRVGPHEEVDEEEAPLQCESSRI-----EISGGQLRVNGRAITVAGANRHEHDDSGGKVVPLESMVRDALVMKRHNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMEPTPSRLTRDPDWQEAHIERLKGMVERDKNHPSIISWSLGNEAGLGAAHYAMSKWLRVRDPSRIIMYEPAMFEGSPPXXXXXXXXXXXXXXQSSSATDVICPMYARVEKCVRFLHEDDRKLAXXXXXXXXXXXXXRPLILCEYSHAMGNSNGNLHKYWELFRSNPRCQGGFVWDWVDQGLRKTIPGPPGGAGG--------VETWGYGGDFGEPVNDGNFCVNGVVWPDRKPHPAMEEFKYLMQPFHASILPGGVTTTTPRPSAPSP-------------------------------------------TTVTLSIANRYDFTD--NLWDILVFGWHVEVDGVVVAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGAGRVTAATLTFESLPSLTGGVEKECWLTVTGRLRAATAWAPAGYKVGHVQLELPRS------------------------------------------------PES-----------------SALQLLGGDEG-GAVDLLEAVENDDDDDGVNGEDEHSRRALGLQFNRAPTDNDRGGYVGRWDVAGLLEPALGPFGARSGWERREADGAVLVTTEFKLRPRAPKPRLCKLFYKV------------------------------SFVRATAAAWRLGHAAEDNSGGSGKG---------VRVWLPPVRLNSTHDVAAGMSVAPETAVG-----------------------DAEGVGXXXXXXXXXXAEGVEDFDAGEFECRVCYAVLPSGVLTAESDVSMPEHWPVVPRVGIRVLLPGSFSSIRWFGRGLHENYPDRKASTVVTRHASSVAEQLTPYIRPGECGGKADVRWLEL------------------------------------------------------------------PPRP---VTAVSLDHRIMGVGGDDSWSACVHDEYLV 1330          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A6H5KC76_9PHAE (Beta-galactosidase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KC76_9PHAE)

HSP 1 Score: 1129 bits (2921), Expect = 0.000e+0
Identity = 640/1231 (51.99%), Postives = 752/1231 (61.09%), Query Frame = 2

Query: 2252 PVAQASCSTAGLLVGVYLKVSRPKLWTAERPHLYTLVVSTHTVGPDGKV-DEESPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPKPSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQR------------------SATDVLCPMYARVEECVRLVKADAKR------------GGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSK-----------------DGEKAVASGDGGGGERGGWGRGEVATVRVEFEA-PAAWTADALELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLA-EIGRAAELA--------LASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGAR--------DGEQSLRGLSLQFHRAPTDNDRGGYEGQWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVES-----ETPLGTSSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQ--------------------PSXXXXXXEAAVVFTVPRNEAGVGGEGAKSS----------------SNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAPFWRE---VCDDGDDGVGNSADRQAIERWRSLRKDDE 5614
            PVA+ASC+T+G+ VG+YLKV RP+LWTAERPHLYTLVVS H VGP G+V +EE+PLQCESSRVGFRK+E+SGGQL+VNGR ITVAGANRHEHDD GGKVVPLESMVRD L+MKR NFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGM+P PSRL++DPDW+ A +ERL+ MVERDKNHPSII+WSLGNEAG+GP HY M+ W R RD SR IMYEPAM EGS                     SATDV+CPMYARVEECVR ++ D ++                RPLILCEYSHAMGNSNGNL KYW+LFRS+P+CQGGFVWDWVDQGL TT+P       G  G +   VE WGYGGDF EP+ND NFC+NGVVWPDRTPHPA+EEFK+LMQPFH +++P+G+    L  S+                                                ++L++ NRYD  D  +LWD+L F W +E DGVV+ARG+DLS  PS SP                         G  A A      GE GG G G V    + FE+ P+       E WLTVTGRLR    WA AGH VGH QLELP + E    AE +                                                   G  G+RAVFD + G L++LQLLG   G    D ++LL+             +GE S R L LQF+RAPTDNDRGGY G+WD AGLL PALGPF  +  W RR+ DGAV+VTTEFKLRPR PKP LC++  K+R FRD+ E+               L L+ L++DE SFVR+ AA WRLG A   N    G GG+G      VRVWLP VRL++THD+A GMS+APE            G  E  GGGD+    D DGV    E FDAGE  C++ Y + P G L VE DV+MPEHWPV+PRVGLR LLP  FSSIRWFGRGPHENYPDRKAS  +  HAS+VA+QLTPYIRPGECG K+DVRWLE+ RP Q                    P+      E A++F VP   A   G                      ++     F+FSALP LAEDL+G  H EQL PPRP   +TAVSLDH +MGVGGDDSWS+ VH+E+LVRPGRFRF+F++APFWR    V D+G  G  N    QA E WR+LR+  E
Sbjct:   40 PVARASCTTSGMYVGLYLKVPRPQLWTAERPHLYTLVVSMHRVGPHGEVNEEEAPLQCESSRVGFRKIEISGGQLRVNGRAITVAGANRHEHDDSGGKVVPLESMVRDALVMKRHNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMEPTPSRLTRDPDWQEAHIERLKGMVERDKNHPSIISWSLGNEAGLGPAHYAMSKWLRVRDPSRIIMYEPAMFEGSPPXXXXXXXXXXXXXXXXXGSSATDVICPMYARVEECVRFLQEDDRKLAKSXXXXXXXXXXXXRPLILCEYSHAMGNSNGNLHKYWELFRSNPRCQGGFVWDWVDQGLSTTIP-------GPPGGA-GEVETWGYGGDFGEPVNDGNFCVNGVVWPDRTPHPALEEFKYLMQPFHASILPNGVTTTTLRPSAPSP-------------------------------------------TTVTLSIANRYDFTD--NLWDILVFGWHVEVDGVVMARGDDLS--PS-SPXXXXXXXXXXXXXXXXXXXXXXXASGFPAAA------GEGGGAGAGRVTAATLIFESLPSLTEGVEKECWLTVTGRLRVATAWAPAGHKVGHVQLELPRSPESSAGAETSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDSGVRAVFDMQSGRLSQLQLLGGDEG----DAVDLLEXXXXXXXXXXVDGEGEHSRRALGLQFNRAPTDNDRGGYVGRWDVAGLLEPALGPFGARSGWERREADGAVLVTTEFKLRPRAPKPRLCKLFYKLRAFRDEAEACSSXXXXXXXXXXTTLSLQSLRDDEVSFVRATAAAWRLGHAAEDN---SGGGGKG------VRVWLPPVRLNSTHDVAAGMSVAPETAV---------GDAEGVGGGDDX--XDADGV----EDFDAGEFECRVCYAVLPSGVLTVESDVSMPEHWPVVPRVGLRILLPGDFSSIRWFGRGPHENYPDRKASTVVTRHASSVAEQLTPYIRPGECGGKADVRWLEVSRPAQNRAPAAAGIGGVSNAAAATAPASSYAFGEPAILFAVPAATATESGXXXXXXXXXXXXXXXXXXXXXXADSSPSLFSFSALPHLAEDLAGAMHPEQL-PPRP---VTAVSLDHRIMGVGGDDSWSACVHDEYLVRPGRFRFSFALAPFWRRRRRVADNG--GCENDGGEQASELWRALRQGKE 1174          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A835ZBT9_9STRA (Beta-galactosidase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBT9_9STRA)

HSP 1 Score: 762 bits (1968), Expect = 1.170e-239
Identity = 592/1615 (36.66%), Postives = 747/1615 (46.25%), Query Frame = 2

Query: 1238 WQNPQVLGFNKLRARTTVGAFSSVEQARANVELPTASENVLSLDGTWRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQ---GYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLS---------SSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVAQASC--------STAGLLVGVYLKVSRPKLWTAERPHLYTLVVSTHTVGPDGKVD------------EESPLQCESSRVGFRKVEVSG--------------GQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPKPSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAM-----------------LEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLF-RSHPQCQGGFVWDWVDQGLKTTVPR----VSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQL-EADGVVVARGE-DLSRIPSHSP---------------------LSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAPAAWTADALELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTK--GGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSL--QFHRAPTDNDRGGYEGQWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKL------------------------------RPRDPKPALCRMLCKVR----DFRDKVESETPLGTSSQ---------------ALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGG---------------------------GGDNVENSDGDGVGE--------------EGEGFDA-----GEIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSA-FSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSV 5509
            W+NP V G N+L  R  +G+F     A  +V  P AS    SLDG W F L  SPE+A+ + F+R   Q D   A +            W+   VP CWQLQ   G D PIYTN +YP PV PP VP  NPTGCY     V   W         R+I+LHF GVD AF+ WVNG L GFSKDSRLPAEFD+T+  A  ++  N ++VM VRWSD ++LEDQDHW LSGI+RSV LVALP   A++D+SWQA +          S T    A + V C        ++TD +     A V V A LFE+G++P              XXXXXXXXXXXXXXXXXXX                    S     V +   VS P+LW+ ERP+LYTLV+ST + G                  E   LQ ES RVGFR + + G              G L+VNG RI V GANRHEHDD GG  V   +MV+DVLLMKRFNFNAVRTSHYPN P+FY++C R GLYV+DE NIETHGMQP P  LS  PDW+GA+++R+Q MV+RDKNHP +IAWSLGNE+G G  H  MA W R  D SR +MYEPA                  +  +A   ATDVLCPMY RV EC  L            PLILCEYSHAMGNS G LDKYW+LF     +CQGGFVWDWVDQG++  V        K   G   DS+  VE W YGGDF+EP+ D +FCING  WPDRTPHPA+ EFKHL +PF              T+S+                               SG G            +I L + NRY H    SL   L F W+L  + G VV   +  LS  P+++P                            +  K V        E GG    E   VR      A W     +L +T+      +AP         H     PLA +      A  S+     L    + GT  P+   +  +   L+   S  EG G   ++  +  +  GG +   +L                     + GE + R  ++  QFH     N   GY     AA     A+ P                                                      RP    P +   L +VR    ++   +     LG  +Q               ++ + GL  +E +FVR++AA +RLG A     +      +G      VR+W+P+V   TTH + EGMS APE  +    A  EP                                  G     ++                     E +  DA     GE+ C+ +Y +   GAL VE  V++P  WPV+ RVG+R LLP+   + ++W GRGPHENYPDR ASAP+G + S+V D   PYIRPGECGA+    W+   +P                  P + A      A S S    + FAFSA   L EDL  V H E+L    P  P TAV+LDH +MG+GGDDSWS+SVH E L+ PG FRFAF +
Sbjct:   15 WENPLVTGRNRLPPRPILGSFPLQGLAYGHVGAPEASPYTQSLDGDWNFKLFGSPEEALTTQFWR---QSDHSPAEQ------------WRPLAVPGCWQLQLEPGADPPIYTNIQYPIPVTPPRVPERNPTGCYVRTVEVAQGWAG-------RRIVLHFGGVDSAFYVWVNGQLQGFSKDSRLPAEFDVTE--ALNTTGANTVAVMVVRWSDGAFLEDQDHWHLSGIYRSVHLVALPSNGAITDYSWQADVQLHPQDRSAESVTAAAAATVTVLC------DVQSTDNS----GADVEVAASLFEDGVVPLPTLAGSSGTSDSTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGVAQRVTLQFVVSLPQLWSPERPYLYTLVLSTSSSGSSSSAQLGRSNXXXXXXXELQVLQSESCRVGFRVMTIGGAAISGNGGSRDGGDGVLRVNGARIVVNGANRHEHDDVGGYAVTRATMVQDVLLMKRFNFNAVRTSHYPNDPWFYDLCSRAGLYVIDETNIETHGMQPYPGYLSDHPDWKGAYLDRVQRMVQRDKNHPCVIAWSLGNESGYGTNHDAMAEWVRSVDTSRLLMYEPACYGIPKARNDGTTASVGSIIAAAPVPATDVLCPMYLRVNECRDLAATRPHA-----PLILCEYSHAMGNSCGGLDKYWELFWEPGGRCQGGFVWDWVDQGIRKKVNAGGGGYGKCSHGLTPDSNGMVETWAYGGDFNEPVTDYDFCINGATWPDRTPHPALYEFKHLAKPF--------------TASTAAVPIPL------------------------SGAGTH----------RIPLKLVNRYQHTP--SLAATLTFGWELLSSTGHVVGSEQLSLSLAPANTPPPTAPLTXXXXXXXXXXXXXXXXXXXXSEVTKPVLMR-----EEGGQLVAEAGGVR------AVWNTATAQL-VTLQHVTGSSAPQDLLYSEAEHCDTS-PLAPLSLQLHRAPTSNDRGGYLALWAAAGTSGPL---DGPYDSTLSW--SQREGDGAAVVKTAYTLRPHGGNVRLCELA-------------------VKVGELASRAPTVRQQFHGERVHNQANGYV---RAAAAAVTAMAPSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHARRALLLARPLSLAPVVVGALHQVRLLILEYVQHLAVAQLLGVLAQPHPETDGVVSSVALASMEMAGLTSEETTFVRAVAAAYRLGHAPLPAAQ------QG------VRLWVPSVPSCTTHPIGEGMSAAPEDTSPATEA-HEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGSTTTSSAQPXXXXXXXXXXXXXXXXXXLEAQNADALLHALGEVSCEALYVLDGSGALSVEYSVSVPPTWPVLARVGVRLLLPAEPLTHVQWLGRGPHENYPDRLASAPVGLYESSVRDLFVPYIRPGECGARCYTSWVTF-KP-----------------APASAADGDSSSAPSLSIAVAEPFAFSAHRLLPEDLD-VKHPEEL----PARPFTAVNLDHSIMGIGGDDSWSASVHAEHLILPGHFRFAFKL 1464          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A2P6V4N2_9CHLO (Beta-galactosidase n=1 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6V4N2_9CHLO)

HSP 1 Score: 644 bits (1662), Expect = 7.150e-197
Identity = 516/1482 (34.82%), Postives = 675/1482 (45.55%), Query Frame = 2

Query: 1238 WQNPQVLGFNKLRARTTVGAFSSVEQARANVEL---PTASENVLSLDG-TWRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLS---SSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVAQASCSTAGLLVGVYLKVSRP------KLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPKPSRLSKDPD----WRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGL-KT-TVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATV------RVEFEAPAAW------TADALELWLTVTGRLRKNAPWAAAGHVVGHTQLELP-----LAEIGRAAELALASSQSKSSLVRGTSFG-TRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRG-LSLQFHRAPTDNDRGG-----YEGQWDAAGLLGPALGPFDTKCSWARRK---VDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVESETPLGTSSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHWPV---------IPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHA-STVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAP 5515
            W+NP V   N+ RA   + +F++ EQA  +  L    + S +VLSL+G  WRF L   PE A+ + F   GF  DA                SW    VP+ W+ QG+  P YTNF YPFPV PP VPA NPTGCY+LAF  P       A +   + +L F GVD AF+ W+NG   G+S+DSRLPAEFD+T  +  G   +NVL+V  ++WSD SYLEDQD WWLSGIHR V L+  P    ++DF  +  L    +S  V+ A L V   +E  ++ +    A       V  R  L +E                                     AD  G        + G  V     ++ P      +LW+AE PHLY L++S   V  DG    + P++ E+ +VGFR  EV G QL  N   + + G NRHEHD   GK + LESMVRD+ LMKR NFNAVR SHYPNH  +YE+C +LGLYVVDEAN+ETHG  P  +    +P     W  A +ER   M+ERDKNHPSI+ WSLGNE+G G  H  MA + R RD SR + YE     G ++  ATDV+CPMYARV +  +L     +     RP++LCEYSH+MGNS GN+D YW  F  HP  QGGF+WDWVDQ L KT T+P      +GR       +E W YGGD+ +  ND+ F  NG+VWPDRTPHPA  E  +L  P  V L   G          XXXXXXXXXXXXXXXXXXXX                          A ++L + N+        L    A  W+L  DG           +P+ +            A  +  GGG E G W    +A            E  ++W         A E  + V  +L ++ PWA AGH V   QL L      LA  G AA        +    ++G + G T   + +E                G GGG     FD   G L+  Q+       GG DP           G + L   L+  F+RAPTDND+GG     Y  +W AAGL    + P   + S    +     GA  V   F LRP +                                   G +E+EA     +A G  +G+                              +   H L+E         AA+E AG   GR+E                         G + C +VY++   GAL V+ +V   +  P          +PRVGL F  PS+   ++W+GRGPHE YPDRKA AP+  H+  +VA    PY+ P E G ++DVRWL L                            GG G  +++     T   S  P          H  +L      +P T V LDH  MGVGGDDSWS +VH+ +LV P +++F   + P
Sbjct:   19 WENPAVTAINRRRAHVPLRSFTAPEQALEHYRLRSEASTSPHVLSLNGDAWRFKLFDLPE-AVPANFGAAGF--DA---------------ASWSQIQVPTNWECQGHGRPQYTNFVYPFPVNPPFVPADNPTGCYQLAFDSPA------AAAANARALLVFEGVDSAFYCWLNGKFVGYSQDSRLPAEFDVTAALRPGE--ENVLAVQVLKWSDGSYLEDQDMWWLSGIHRDVYLLLKPSS-HIADFQVRTPLRFNHASGAVQAAGLEVEVAVEGGSAEELQGLA-------VQARLYLCDEA----------GEVDPAAADAALVAEFSAAVESTWVAADTSGVAGAGDARSGGRAVLSLDMLTLPGGAAALRLWSAELPHLYLLLLSL--VEGDGSGSSQ-PIEIEACQVGFRTAEVRGRQLLHNNAPVMLKGVNRHEHDQLRGKTISLESMVRDICLMKRLNFNAVRCSHYPNHTLWYELCSKLGLYVVDEANVETHGFDPALTNNHLNPACSPLWLNAIVERGARMLERDKNHPSILLWSLGNESGYGAAHLAMAGYIRARDASRPLHYEG----GGSRTPATDVICPMYARVHQIQKLAALPDEH----RPVVLCEYSHSMGNSTGNVDAYWAAFEGHPHLQGGFIWDWVDQALLKTETLP------DGRT------LEYWAYGGDYGDSPNDAQFVCNGLVWPDRTPHPAAFELAYLQAPLAVELALSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAD------------------------APLALRLRNKQHFASTADL----ALSWRLLVDG-----------LPAPA------------AGGAAPGGGAEHG-WRPLLLAAPLGPQQEEARLELGSSWGEVVRQARGAAEPLMEVRAQLLRDLPWAPAGHQVQTCQLPLAGLLPDLAATGLAAPGQAPQQVAPLPRLQGLAVGRTDGALRVEG--------------VGAGGGSWSLTFDAATGALSSWQVA-----AGGADP-----------GHELLAAPLTPCFYRAPTDNDKGGSGGSSYTARWKAAGLDRLEVDPASVRLSAPDEQQAVAAGAAHVRCSFALRPAER---------------------------------AGEEEEEA-----VAEGVGVGE------------------------------VGGAHWLSEAQPTEVNVAAAEEAAGS--GRSE-------------------------GSVQCSVVYSVRGDGALHVQWEVDARDALPAPLAAGLDKSLPRVGLCFGAPSSLQHVQWYGRGPHECYPDRKAGAPLRCHSVDSVAQLHVPYVFPSESGGRADVRWLALAERS------------------------GGPGLLAAAVGAGSTLQVSTSPYSVRSFERAKHDHELQ----ASPFTWVHLDHRHMGVGGDDSWSPTVHKPYLVPPAQYQFGLLLQP 1228          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A1Y1IHV0_KLENI (Beta-galactosidase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IHV0_KLENI)

HSP 1 Score: 630 bits (1626), Expect = 6.510e-193
Identity = 480/1471 (32.63%), Postives = 679/1471 (46.16%), Query Frame = 2

Query: 1238 WQNPQVLGFNKLRARTTVGAFSSVEQARA-------------------NVELPTASENVL----------SLDGTWRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSSSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVAQASCSTAGLLVGVYLKV--SRPKLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPK----PSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAPAAWTADAL--ELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQSKSSLVRGTSFGT-RFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSLQFHRAPTDNDRGGYEGQWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVESETPLGTSSQALVLEGLKEDEASFVRSLAAGWRLG---QATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEG---MSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHW-PVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAP 5515
            W++P   G+NK +A   + + +  + A                     +  LP A ++            SL G W+F L   PE+        K F E A+             DGSW D  VPS WQ+ G+D PIYTNF YPFP+ PP VP+ NPTGCYR  F VP ++       E R++ L F GVD A++ +VNG L G+S+DSRLPAEFDI+++ A G      ++V  +RWSD SYLEDQD WWLSGIHR V L A P+   +S   +Q   + S D+  A L V+  +E S S    + A  A+AA   V   L+ +                                      + R     AS    G      LKV  ++PKLW+AE P+LYTLV++       GKV     ++CE+ RVG RKVEV   +L VN + + + G NRHEH    GK +  E+MV D++LMK  N NAVR SHYP HP +YE+CD  G+Y+VDEANIETHG  P      ++L+  P+W+ AF++R   MV+RD+NH SI+ WSLGNE+G GP H  MA W R  D +R + YE     G A+ S TDV+CPMY RV + V++ +   ++    RP+I+CEYSHAMGNSNG++D+YW+ F +    QGGF+WDWVDQGL        K G+  +       + W YGGDF +  ND+ FC+NG+VWP+R+PHPA+ E K+L QP  +T   +GIK                                                            + NR+   D+ S  D L+F W L +DGV +  G     +P+  P +         A+ S                        AP A++  +   E +LT+T     +  WA+ GHVV   Q+ LP    G   E     ++SK     G S G  +  + +++ R TR  +   S         ++  FD   G ++R  + GT          +LL           +RG    F RAP DND+GG E  + A                WA      A  V   F ++P          +  + +   K  S         A  L  L+ +      S  A W+L    +    N E          + S VR  L             G    S   +     E +G  P  ++A  G  +V++S    + E G     G+I   + Y++   G ++ + +V  P+ W P + RVGL  ++P    ++ W+GRGPHE YPDRKASA +G + ST  D   PYI PGECG ++DVRW+ +  P                         G  G  ++    T     +      E L    H E+L P   EA +  V LDH  MG+GGDDSW+ SVH ++L+ P +++++  ++P
Sbjct:   85 WEDPTFFGWNKWKAHVPLHSHTDEDSALRFWFDRSQACKEDAAKALWDDKALPEALKSARQWTSDLPYTQSLSGDWKFHLAPKPEEVP------KDFVEPAYN------------DGSWGDLTVPSNWQMHGHDKPIYTNFVYPFPMTPPYVPSENPTGCYRHTFKVPQEY-------EGRRLFLEFQGVDSAYYVYVNGTLVGYSQDSRLPAEFDISNIAAAGEEC--TIAVQCMRWSDGSYLEDQDMWWLSGIHRDVLLHAKPQ---VSISQYQVETNLSPDLASAELKVKVSVESSES----EPAKRALAAYT-VEGTLYRD--------------------------FAEADTKHKPEIEARLEKRPASEDAVGHAANATLKVLLNQPKLWSAETPYLYTLVLTLRDAS--GKV-----VECEACRVGVRKVEVKEKELLVNNKPVMIRGVNRHEHHPSLGKAMVEENMVADIVLMKANNINAVRNSHYPTHPRWYELCDLFGMYLVDEANIETHGFDPGLHKVKNQLTWFPEWKAAFVDRGARMVQRDRNHASIVLWSLGNESGYGPAHDAMAEWIRAADPTRPLHYEG----GFARTSVTDVVCPMYTRVFDIVKIARDKNEK----RPVIMCEYSHAMGNSNGSIDEYWEAFENTHGLQGGFIWDWVDQGLL-------KLGKDNK-------KHWAYGGDFGDMPNDATFCLNGIVWPNRSPHPAVAEVKYLYQPLAITAEAEGIK------------------------------------------------------------IFNRFYFTDVSS--DSLSFTWSLSSDGVELGSGS--VSVPNIPPRN--------SALLS---------------------HASAPWAFSRQSAKGETFLTLTAVQAGDTRWASKGHVVATQQVTLPEGRKGGTPE---RRNESK-----GMSSGVPKVRVEVKDGR-TRVTSEDDS---------VQVEFDNATGTISRWTVDGT----------DLL-----------VRGPFPSFWRAPIDNDKGGGETSYIA---------------KWA------AGGVNRSFLVKP----------IHFIENVFGKARSVVVSAWPRAARAL--LRAEAFVLNMSYVARWKLAGLDRLEIANAETAVLSQPADHAFSQVRTQLRLEPSKFKRPQKGGSFTQSYFADSTPELEPSGGAP--SQANVGPPSVDHSS-QPIPETGH---EGKIDVSVTYSVYGSGDIVADVEVN-PDTWLPPLARVGLDMVVPKEMKAVEWYGRGPHECYPDRKASAWVGRYKSTAHDLHVPYISPGECGGRADVRWVAISSPD------------------------GARGFVAAPGGITRPLQVNVSEFSNEALEQALHDEELVPD--EAGIH-VHLDHEHMGIGGDDSWTPSVHSKYLLPPIQYKYSIRLSP 1266          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A7S0GT59_MICPS (Beta-galactosidase n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0GT59_MICPS)

HSP 1 Score: 593 bits (1529), Expect = 3.110e-179
Identity = 470/1503 (31.27%), Postives = 630/1503 (41.92%), Query Frame = 2

Query: 1238 WQNPQVLGFNKLRARTTVGAFSSVEQA----------RANVELPTASENVLSLDGTWRFALHASPEKA-IDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEV-------------------------------------PATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSSSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVAQASCSTAGLLVGVYLKVSRPKLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQC---ESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHG----MQPKPSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQP--FHVT-LVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSL---WDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAPAAWTADALELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQS---KSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSLQFHRAPTDNDRGG----YEGQWDAAGL--LGPALGPFDTKCSWARRKVDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVESETPLGTSSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFA-----FSALPC-----------LAEDLSGVAHAEQLSPPRPEAPLTA-VSLDHLLMGVGGDDSWSSSVHEEFLVRPG 5485
            W+NP+V+G  K      + +F  V  A           +  +LP    NVL L+G W F L A+P+ A +D  FF   F                     W +  VPS W+ QGYD PIYTN  YPFP+ PP                                          NPTGCYR AF +P+ W         R++ + F GVD AF+ WVNG   G+S+DSRLPAEFDIT+++  G   KN ++   +RWSD SYLEDQDHWWLSGIHR V ++ L +   +SD+  +  L        AR++V   ++ + +++      VA+   V V AL+F+                                      A+  G               + L V   +LW+AE P+LYTLVV T T    G       +     E+ R+G R V +S  +L VNG  + + G NRH+H  + GK V  ESM++DVLLMK++NFNAVRTSHYPNHP FY++CD  GLYV DEAN+ETHG    M P P  L+  P WR A++ R+  M++RD+NH SII WSLGNEAG G     MA WAR  D +R + YE     G ++ S TD++CPMYARV  C ++   D       RP+ILCEYSHAMGNSNG+LDKYW+ FR +   QGGF+WDWVDQGL+ T P   KT              W YGGDF +  ND+ FCING+V+PDRTPHPAMEE K LM+P  F VT L+ +      + + S                                              +   + V N  +   +D L   W +LA       +GV+     DLS IP  + +  P        V                +V+T+R      AA T+     +L     +++   W  AGH V HTQ+ LP+   G  +   L+  +S    S +VR  + G               L V  +        G+   F   G        +GT            +  +G+R       G S  F RAPTDNDRGG    Y  +W  AG+  L      F T    A    DG++ +     +                          TP G++                                                  R  +  + ++ THD+                                                                G + +    T  +  P IPRVGLR    S   ++ WFGRGPHE Y DRKASA  G H+S   D   PYI PGE G ++DV WL L R                  +P     + G   ++S+  D  T A       ++P              EDL   +H  QL   R     T  V +DHL MGVGGDDSW+ +VH E+L+  G
Sbjct:   92 WENPEVVGRCKRAGHVPLYSFRDVPTALKFWSEGGGWSSRSKLP----NVLLLNGKWDFHLAAAPQDAPVD--FFHSNFDTSL-----------------WDEIDVPSNWECQGYDRPIYTNMFYPFPLDPPRALRRGVWSSSNNSTASSQGGDLSLIGGWKWDPEIKNPNELENPTGCYRRAFDLPEGW-----TENGRRVFISFGGVDSAFYCWVNGQRVGYSQDSRLPAEFDITEVLVPG---KNFVAAQVMRWSDGSYLEDQDHWWLSGIHRDV-ILYLKEAVFISDYMVKTSLPEKRG-GPARVDVDIRVQEAVTSE------VALPTDVIVEALIFDAD-----------------GTQIACVTSRPEDLLWSDPAEVYGQPFDKDQPQFNRKALLSLSVPAARLWSAEVPYLYTLVVRTMTPTATGNTSSRRFVPASDVEACRIGIRSVSISEKKLWVNGVPVIIQGVNRHDHCPKNGKAVSEESMLQDVLLMKKYNFNAVRTSHYPNHPRFYDLCDEYGLYVCDEANLETHGFHAGMHPTPF-LANIPQWRQAYISRMARMIQRDRNHASIIMWSLGNEAGCGSGQRAMAEWARENDPTRPLHYE----SGGSRSSCTDIICPMYARVYTCEKMANEDPNDH---RPVILCEYSHAMGNSNGSLDKYWRCFRENSAVQGGFIWDWVDQGLEATAPS-GKT-------------FWAYGGDFGDKPNDAQFCINGIVFPDRTPHPAMEELKFLMRPVTFQVTNLLKEEANCTRVKTDSELVNLETW------------------------------------ISSNPRIVVRNWLNFTSLDHLKTTWLILADSGARLEEGVL-----DLSSIPQDAEVELPWKAFLPDLVFLAK------------KVSTLRT-----AAQTSPLHNWFLEFRTTVKETTRWCDAGHEVAHTQITLPVPVNGFPSPSMLSIPKSLSVHSDMVRVFTLGDDL------------LQVQCA-------DGVHMAFHLSG------PKIGT------------IVNMGSRGKLILEGGPSPCFWRAPTDNDRGGEAISYCARWRNAGINQLQTVQDSFPTVTQTAGP--DGSLRIAVTLTM--------------------------TPCGSN--------------------------------------------------RKGVAGISVTLTHDIQTN-------------------------------------------------------------GIVDLNMVATASKSLPPIPRVGLRMRCSSELQAVDWFGRGPHECYSDRKASATFGHHSSNAVDMHVPYIVPGENGGRADVSWLALRRRE---------------VIPEQPLTIKGNKLRASARTDRGTVAPAPGLLVSIPAGKVAQVSVQQHSLEDLESSSHEYQLEESRSRGDGTVHVHVDHLHMGVGGDDSWTPNVHPEYLIPTG 1267          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A4P1R0G2_LUPAN (Beta-galactosidase n=3 Tax=Lupinus TaxID=3869 RepID=A0A4P1R0G2_LUPAN)

HSP 1 Score: 585 bits (1509), Expect = 4.980e-176
Identity = 473/1495 (31.64%), Postives = 648/1495 (43.34%), Query Frame = 2

Query: 1355 VLSLDGTWRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMI-AFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSSSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVA-QASCSTAGL------LVGVYLKVSRPKLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPKP--SRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAP---AAWTADALE-LWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSLQFHRAPTDNDRGG----YEGQWDAAGL--------------------------LGPALGPFDTKCSWARRKVDGAVV----VTTEFKLRPRDPKPALCRMLCK--VRDFRDKV--------ESETPLGTSSQALVLE-GLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTT--------HDLAEGMSLAPEQGAAKEGAGDEPG------RNEAGG------------GGDNVENSDGDGVGEEGEGFDAGEIGCKIV-----------------------YTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAP 5515
            V SL G W+F L  SP    D  FF   FQ                 D  WK  PVPS WQ+ GYDVPIYTN  YPFPV PP VP  NPTGCYR  F +P +W       E R+I+LHF  VD AF AW+NG   G+S+DSRLPAEF+ITD     GS  KNVL+V   RWSD SYLEDQD W LSGIHR V L+A PK   ++D+ ++++L+   D   A + V   ++ S      +     +     V A L++ G                                    + N   +  Q S +T  L      LVG   K+  PKLW+AE+P LYTLVV         K      + CES  VGFR V  +  QL VNG+ + + G NRHEH  R GK      M++D++LMK+ N NAVR SHYP HP +YE+CD  G+Y++DEANIETHG          + +P W  + ++R+  MVERDKNH  II+WSLGNE+G G  HY +A W RGRD SR + YE     G ++   TD++CPMY RV + +++    A+     RPLILCEYSHAMGNSNGNL  YW+   +    QGGF+WDWVDQ L     ++ + G          V+ W YGG+F +  ND NFC+NG+ +PDRT HP + E K+L QP  V L                                                 +DG+           L + N +     + L     F W   ADG  +  G     + S +P+    S                             +++++    + W + + E ++LT+T +L  +  W  AGHV+  TQ++LP     R   +  A S S  +LV  T   T         + ++K    ++              +TK G +   ++ G                          +G+   F RA  DND+GG    Y  +W AAG+                          LG   G  D         V   +     V  E  ++P    P L R+  +  V    D+V        ES      S+Q  V E  +K+    +V       R      T     G G   +  G++  + + A   ST+        H L EG S+  + G      G  P        N+ GG            G DN+       V E     +  E   KI+                       YT+   G +I+EC+V      P +PRVG+ F +  +   + W+GRGPHE+YPDRKASA +  +   V D   PY+ PGEC  ++DVRW+ L                      RN+ G G     +S   ++     SA      +L    H  +L     E     V LDH  MG+GGDDSWS  VH+++LV P  + F+  ++P
Sbjct:   87 VKSLSGHWKFFLADSPNTVPDK-FFESDFQ-----------------DLEWKFLPVPSNWQMHGYDVPIYTNVVYPFPVDPPLVPIENPTGCYRTEFHIPKEW-------EGRRILLHFEAVDSAFCAWINGHPIGYSQDSRLPAEFEITDFCHPCGSDLKNVLAVQVFRWSDGSYLEDQDQWRLSGIHRDVILLAKPK-VFITDYFFKSILAE--DFSSAEILVEVKIDNSQETSKDN-----ILTNYTVEAALYDSG------------------------SWYTCDGNTDLLSSNVADIKFQPSTATTPLGFHGYTLVG---KLQSPKLWSAEQPFLYTLVVVL-------KDQSGHIIDCESCSVGFRNVTKAHKQLLVNGQAVVIRGVNRHEHHPRVGKANIESCMIKDLVLMKQNNINAVRNSHYPQHPRWYELCDLFGMYMIDEANIETHGFDYSKHLKHPTLEPIWATSMLDRVIGMVERDKNHTCIISWSLGNESGFGHNHYALAGWIRGRDSSRVLHYEG----GGSRTPCTDIVCPMYMRVWDMLKIANDPAET----RPLILCEYSHAMGNSNGNLHTYWEAIDNTFGLQGGFIWDWVDQALL----KMDENG----------VKRWAYGGEFGDIPNDLNFCLNGLTFPDRTAHPVLHEVKYLYQPIKVIL-------------------------------------------------HDGK-----------LEIKNTHFFQTTEGL----EFSWYASADGYNLGSG-----VLSLAPIKPQSS---------------------------YTIDWQSGPWYSLWASSSSEEIFLTITAKLLNSTLWVEAGHVISTTQVQLP----ARKDIVPHAISISGGTLVAETLGDTI--------KVSQKDVWDITL-------------NTKTGSVESWKVKGVNV---------------------MSKGILPSFWRASIDNDKGGESASYLSRWKAAGIDNLHFVTERCSIVNTAEDTVKILVVFLGITKGDQDKSNILFTTDVSYTIYASGDVIMECNVKPNLDLPPLPRVGIEFNVEKSLDQVTWYGRGPHESYPDRKASAQVAVYEKNVKDLHVPYVVPGECSGRADVRWVTLRNKNGFGIYASRYGNSPPMQMSASYYSTSELDQATHNHKLIEGDSIEVK-GVNVMSKGILPSFWRASIDNDKGGESASYLSRWKAAGIDNLHF-----VTERCSIVNTAEDTVKILVVFLGITKGDQDKSNILFTTDVSYTIYASGDVIMECNVKPNLDLPPLPRVGIEFNVEKSLDQVTWYGRGPHESYPDRKASAQVAVYEKNVKDLHVPYVVPGECSGRADVRWVTL----------------------RNKNGFG---IYASRYGNSPPMQMSASYYSTSELDQATHNHKLI----EGDSIEVHLDHKHMGLGGDDSWSPCVHDQYLVPPVPYSFSLRLSP 1315          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A2C9W5H2_MANES (Beta-galactosidase n=2 Tax=Crotonoideae TaxID=235631 RepID=A0A2C9W5H2_MANES)

HSP 1 Score: 571 bits (1471), Expect = 3.440e-173
Identity = 431/1407 (30.63%), Postives = 598/1407 (42.50%), Query Frame = 2

Query: 1355 VLSLDGTWRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKN-VLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSSSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVAQASCSTAGLLV-----------GVYL--KVSRPKLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQ--PKPSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAP--AAW-TADALELWLTVTGRLRKNAPWAAAGHVVGHTQLELP-LAEIGRAAELALASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSLQFHRAPTDNDRGGYEGQWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVESETPLGTSSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAP 5515
            V SL G W+F L  SP K I   F+   FQ                 D  W+  PVPS WQ  G+D PIYTN  YPFP+ PP VP  NPTGCYR  F +P +W       +  +I+LHF  VD AF AW+NG   G+S+DSRLPAEF+ITD      S KN VL+V  +RW D SYLEDQDHWWLSGIHR V L+A P+   ++D+ +++ L+ +    + ++ V+          T +     + A   V A L++ G                                     ++ G     S   A LLV           G  L  K+ +P LW+AE+P+LY LV++       G V     + CES  VG ++V  +  QL VNG  + + G NRHEH  R GK      MV+D++LMK+ N NAVR SHYP HP +YE+CD  G+Y++DEANIETHG          + +  W  A ++R+  MVERDKNH  II+WSLGNE+  GP H   A W RGRD SR + YE     G ++ ++TD++CPMY RV++ V++ K   +     RPLILCEYSH+MGNSNGN+D+YW+   S    QGGF+WDWVDQGL       SK               W YGGDF +  ND NFC+NG+ WPDRTPHPA+ E K+L QP  V+L    IK   +T++                              F +  G                                 L F W +  DG  +  G  +  +P+  P S+ D K                  W             AP  + W ++ A E +LT+T +L  +  W  AGHV+  TQ++LP   EI      A A+  + ++ +   +F       + + +F                             +T     GT   +  W  +E +  +         +G+   F RAPTDND+GG E  +                C W    +D       +F+ R            C +++  D +                                                            V +  V     H  +   +L                                                  + YT+   G LI+EC+V      P +PRVG+ F L  + + IRWFGRGP E YPDRKA+A +G +   V D   PYI PGEC  ++DVRW+                        +++ G+G     +S+   +     SA      +L    H + L     +     V LDH  MGVGGDDSW+ SVH+++L+    + F+    P
Sbjct:   87 VKSLSGFWKFFLAPSPTK-IPKNFYDPAFQ-----------------DSEWETLPVPSNWQTHGFDRPIYTNVIYPFPLDPPHVPEDNPTGCYRKCFQIPKEW-------QGLRILLHFEAVDSAFCAWINGVPVGYSQDSRLPAEFEITDYCYPCDSGKNNVLAVQVLRWCDGSYLEDQDHWWLSGIHRDVLLLAKPQ-VFIADYFFKSNLAENFTSADIQVEVKI-------DSTREIPKDKIFANFTVEAGLYDTG----------------------------------SWYNSDGCADLLSSKVADLLVNSSFDAILGFLGYVLVGKLEKPNLWSAEQPNLYILVLTLKDAS--GHV-----VDCESCLVGIKQVSKAPKQLLVNGCPVMIRGVNRHEHHPRVGKTNIESCMVKDLVLMKQNNINAVRNSHYPQHPRWYELCDVFGMYMIDEANIETHGFHLCGHLKHPTSEQSWATAMIDRVIGMVERDKNHACIISWSLGNESSYGPNHSAAAGWVRGRDTSRLLHYEG----GGSRTTSTDIICPMYMRVQDIVKIAKDPTEP----RPLILCEYSHSMGNSNGNIDEYWEAIDSTLGLQGGFIWDWVDQGLLKESSDGSKH--------------WAYGGDFGDTPNDLNFCLNGLTWPDRTPHPALHEVKYLYQPIKVSLQQSTIK---ITNTHF----------------------------FETTQG---------------------------------LEFSWAVHGDGHELGSG--ILSLPAIKPQSNYDIK------------------W-----------ESAPWHSLWASSSAGEFFLTITAKLLHSTRWVEAGHVISSTQVQLPPRKEIIPYVIKAKAADATLATEIIDDTF------RVSQQKFWE---------------------------ITLNIQTGT---IESWK-VEGVTVMN--------KGIFPCFWRAPTDNDKGGDENSY---------------YCRWKAAHIDNL-----KFETRS-----------CTIQNTSDHL------------------------------------------------------------VKIEVVYAGIEHGSSAHNALFK----------------------------------------------VDMTYTIFSSGDLIIECNVIPNSDLPPLPRVGVEFNLEKSMAKIRWFGRGPFECYPDRKAAAHVGIYEKNVGDMHVPYIVPGECSGRADVRWVSF----------------------QDKDGIG---IFASTYGSSPPMQMSASYYSTAELHQARHNKDLV----QGNNIEVHLDHKHMGVGGDDSWTPSVHDKYLLPAVPYSFSVRFCP 1091          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A7S1CUJ2_9CHLO (Beta-galactosidase (Fragment) n=1 Tax=Picochlorum oklahomense TaxID=249345 RepID=A0A7S1CUJ2_9CHLO)

HSP 1 Score: 560 bits (1444), Expect = 1.370e-168
Identity = 448/1459 (30.71%), Postives = 625/1459 (42.84%), Query Frame = 2

Query: 1238 WQNPQVLGFNKLRARTTVGAFSSVE---QARANVELPTASENVLSLDGT--WRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVKPPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSSSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEE--GIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPV-----AQASCSTA-GLLVGVYLKV--SRPKLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQCESSRVGFRKVEVSG-GQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQP--KPSRLSK--DPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAPAAWTADA--LELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSLQ--FHRAPTDNDRGG-----YEGQWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKLRPRDPKPALCRMLCKVRDFRDKVESETPLGT----SSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMSLAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAGEIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAP 5515
            W+NP V G NK +    + ++ ++    Q  + ++  + S   + LDGT  W+F    SPE ++   F    F                  D  WK  PVP  W++ GY  P Y NF YPFPV PP VP +NPTGCYRL F+ P+D+   DAN+      L F GVD AF  W+NG   G+S+DSRLPAEFDI+  +     ++NVL+V  ++W D SYLEDQD W LSGIHRSV  + L K   + DF                  V+  L+   SA   D     V+  VHV  +  E+  G  P                                  +   PV       +SC  A GL   + ++    R +LW+AE P+ Y LV+             +  ++ E+  +GFRK  +S  GQL  NGR I + G NRHEH     + + L +M  D +LMK++NFNAVR SHYPNH  +Y +C   GLYVVDEAN+ETHG  P  K +R++    P+W GA ++R   M ERDKN PSI+ WSLGNEAG GP H  MA + R RD +R I YE     G ++  ATD++CPMYARVE+ V+++  D       RP++LCEY+H+MGNS GN+D YWK F  H +CQGGFVWDW DQ L  + P  ++T           +  W YGGD S+  +D  F  NGVV+PDR+  PA  E K +  P   +L   G                                              DG          +S+ V N++  + ++         W++  +G   +   D   I     LS   +K  E                    + ++ V  E+ A    D   +E  +     L K   WA  GH + H QL L         +++      + S   G +      +  EE+        A   P       +    DT+ G +    +                       G +++    LQ   +RAPTDNDRGG     Y  +W AAGL    L P    C+ +  KV  A ++T + +L PRD  P       +  D  D+      LG     S +A      K+DE +    L     +     T+G             + +  W     +  TH L + +                                                          P G L            P +PRVG+RF +P  +++I ++GRGPHENYPDR+ASA +  H + V D  TPYI PGECG ++D RW+      + S       + V  T  +  A                   FS+     E+L    H  +L     E    +V +D   MGVGGDDSWS SVHEE+LV+P  + F   ++P
Sbjct:   24 WENPHVFGINKRKPHVPLQSYDNLTDLTQYYSRLQDASMSRRCICLDGTDTWKFHFCDSPE-SVPLEFHSSTFD-----------------DSDWKHMPVPGVWEMNGYGKPQYLNFNYPFPVDPPFVPRSNPTGCYRLRFTTPEDF---DANA-----FLVFDGVDSAFDCWLNGDYLGYSQDSRLPAEFDISAHLN-APGTENVLAVRVIKWCDGSYLEDQDMWRLSGIHRSV-CILLKKEVYIQDFE-----------------VKTHLDFDQSANRVDH----VSLDVHVDIVSNEKESGHSPYNYDLLSVNAHLFEYHYGESIDVEHPLAATQASLE---PVWLTKSCHSSCRRANGLRAEIKIEQLEDRVQLWSAETPNCYLLVLCLQ--------HGDDIIEYEADIIGFRKTWISETGQLLHNGRTIMIRGVNRHEHCPMTARTMTLSAMRHDAVLMKQYNFNAVRCSHYPNHDLWYRICSIYGLYVVDEANVETHGFDPGLKNNRINPACSPEWTGAILDRGIRMYERDKNIPSILLWSLGNEAGYGPAHLAMAGYIRARDTTRPIHYEG----GGSRTPATDIICPMYARVEQ-VKMLAEDINDA---RPVVLCEYAHSMGNSTGNVDTYWKTFEKHAKCQGGFVWDWADQALYDSTPSETET-------ESTTIPRWAYGGDHSDAPHDGQFICNGVVFPDRSHKPASFEMKCVQAPIQTSLEHTG----------------------------------------------DGT---------LSIRVTNKHAFLPLEGFCGF----WRVLHNGAPSSTCGDWRHISMTDHLS---TKMNENTY-----------------IVSLGVSLESLATDQPDEAIVESMVECAWMLPKQTLWAPEGHCIAHDQLLLD--------QISHIKPLEQDSEYIGLTESDPSSLQEEENAMAYHGQTASQEP-------VHLTIDTQTGNIISYTV-----------------------GNRTILDQHLQACLYRAPTDNDRGGSGGKSYAARWKAAGLHN--LQPQG--CTISHSKVGDASLITCKVRLHPRDTPP-------EQMDHTDEGVGVGELGGAHWFSEEAHEDNDSKDDEKTS--ELCVDVHVDYTIRTDG-------------TLMSTWS----IDCTHALPKSL----------------------------------------------------------PKGLL------------PSLPRVGVRFGIPKEYNNITYYGRGPHENYPDRRASALLQQHHAQVPDLHTPYIYPGECGGRTDTRWITW----KNSSGQTISSSLVGRTHQKTPA----------------YCQFSSSLYSMEELDRKRHNHELQ----EGDSISVHMDVAHMGVGGDDSWSPSVHEEYLVQPSTYSFTLMLSP 1166          

BLAST of mRNA_A-nodosum_M_contig1167.22.2 vs. uniprot
Match: A0A3M1H214_9CHLR (Beta-galactosidase n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A3M1H214_9CHLR)

HSP 1 Score: 549 bits (1415), Expect = 4.240e-165
Identity = 453/1453 (31.18%), Postives = 615/1453 (42.33%), Query Frame = 2

Query: 1238 WQNPQVLGFNKLRARTTVGAFSSVEQARANVELPTASENVLSLDGTWRFALHASPEKAIDSGFFRKGFQEDAFAAGKGDTGGVRGADGSWKDTPVPSCWQLQGYDVPIYTNFRYPFPVK--PPEVPATNPTGCYRLAFSVPDDWGRGDANSEKRKIILHFAGVDGAFFAWVNGCLAGFSKDSRLPAEFDITDMIAFGSSSKNVLSVMAVRWSDASYLEDQDHWWLSGIHRSVRLVALPKRCALSDFSWQAVLSSSTDVKEARLNVRCLLERSASAKTTDAAPVAVAAPVHVRALLFEEGIMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADNRGPVAQASCSTAGLLVGVYLK-VSRPKLWTAERPHLYTLVVSTHTVGPDGKVDEESPLQCESSRVGFRKVEVSGGQLKVNGRRITVAGANRHEHDDRGGKVVPLESMVRDVLLMKRFNFNAVRTSHYPNHPFFYEVCDRLGLYVVDEANIETHGMQPKPSRLSKDPDWRGAFMERLQDMVERDKNHPSIIAWSLGNEAGMGPTHYDMAAWARGRDLSRAIMYEPAMLEGSAQRSATDVLCPMYARVEECVRLVKADAKRGGGGRPLILCEYSHAMGNSNGNLDKYWKLFRSHPQCQGGFVWDWVDQGLKTTVPRVSKTGEGREGDSDARVEAWGYGGDFSEPMNDSNFCINGVVWPDRTPHPAMEEFKHLMQPFHVTLVPDGIKAKALTSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXKAFSSGGGNDGEDGGGRFLAKISLAVHNRYDHVDMDSLWDLLAFDWQLEADGVVVARGEDLSRIPSHSPLSDPDSKDGEKAVASGDGGGGERGGWGRGEVATVRVEFEAPAAWTADALELWLTVTGRLRKNAPWAAAGHVVGHTQLELPLAEIGRAAELALASSQSKSSLVRGTSFGTRFPIAIEEHRFTRKLAVAVSFPEGGGGGGIRAVFDTKGGFLTRLQLLGTGFGVGGWDPIELLQPLGARDGEQSLRGLSLQFHRAPTDNDRGGYEG-----QWDAAGLLGPALGPFDTKCSWARRKVDGAVVVTTEFKL---RPRDPKPALCR---MLCKVRDFRDKVESETPLG--TSSQALVLEGLKEDEASFVRSLAAGWRLGQATATNGEGKGNGGEGTSSGSTVRVWLPAVRLSTTHDLAEGMS----LAPEQGAAKEGAGDEPGRNEAGGGGDNVENSDGDGVGEEGEGFDAG---EIGCKIVYTMTPFGALIVECDVTMPEHWPVIPRVGLRFLLPSAFSSIRWFGRGPHENYPDRKASAPIGTHASTVADQLTPYIRPGECGAKSDVRWLELIRPRQPSXXXXXXEAAVVFTVPRNEAGVGGEGAKSSSNDDTDTFAFSALPCLAEDLSGVAHAEQLSPPRPEAPLTAVSLDHLLMGVGGDDSWSSSVHEEFLVRPGRFRFAFSVAP--FW 5521
            W+ P V+  N+  A  T+  +     A     +  AS     L+G WRF L  +P+ A    F R  +                  D  W    VP  WQLQGYD+P YT+ + PFP+   P   P  NPTGCYR  F++P+ W         R++ L F GVD AF  WVNG L G+S+DSRLPAEF+IT  +  GS   N L+V   RWSD +YLE+QD W LSGI R V L A P    + DF  Q     +   ++A L  +  L R       D           +   LF+    P                                      P A+ +    G     +++ V+ P  W+ E+P+LYTL++    VGPDG++     L+ ES RVGFR+VE+  GQL +NG+ I + G NRHEH    G  V   +M+ D+ LMK+FN NAVRTSHYPNHP +YE+CD  GLYV+DEANIE  G      RL+ DPDW+ AFM R+  MVERDKNHP +I WSLGNE+G G  H   A W R RD SR I Y PA           D+L PMY  V+  +++ +    R     P+I+CEY+HAMGN+ GNL +YW+    +P+ QGGF+WDWVDQGL+  +P      +G+E         + YGGDF +  ND +FC+NG+ WPDR PHPA+ E K + +P  V  V                                                          LA   L + NR+   ++          W++EA G V+A+G+          L  PD   G+ A                     + V F  P   +    EL+L++   L  + PWA AGH +   Q  LP          A  S++  + L          P+    H  T +  + V+      G      FD + G L   Q       VGG    + L   G R            F RAPTDND G +       QW  AGL   A    +T+   A    DG V VT    +   RP +P P   R    L ++ D   +  +   L    +   + L          VR+L +            E            +  R  L A    T   + + +     L+P+   A                                  F A       C   Y ++P GA+ +E  V   +  P +PRVGL   LP  +    W+GRGPHE YPDRK  A +G +  TV +Q TPYI+P E G+K+DVRW EL                       ++AG G         +       S       DL+   H   L P R EA L    LDH   G+G ++S    V   +L+    +R+A  + P  FW
Sbjct:   11 WETPAVVERNREPAHATLMPYPDDTLALQGNRM--ASPFCQLLNGKWRFHLAPNPDAAPPD-FPRPDYD-----------------DSGWDTVTVPGNWQLQGYDIPYYTDLQLPFPLDYLPGVPPDDNPTGCYRRRFTIPESWAG-------RQVFLTFEGVDSAFHVWVNGRLVGYSQDSRLPAEFNITPYLHPGS---NTLAVRVYRWSDGTYLENQDMWRLSGIFRDVYLWAAPD-IHIRDFHIQTRFDPA--YRDAVLTAQVSLRRYTGHPAADC---------RLELHLFDAAGKPLWFDP---------------------------------PAAETAVPLGGETSLHFVQPVAAPHPWSDEQPYLYTLLL--RLVGPDGRL-----LEVESCRVGFRQVEIINGQLCLNGKPILIRGVNRHEHHPDTGHTVDEATMLADIRLMKQFNINAVRTSHYPNHPRWYELCDEYGLYVLDEANIECDGAL---DRLAHDPDWQTAFMARVSRMVERDKNHPCVIIWSLGNESGFGANHRAAAEWVRARDPSRPIHYHPA-----GNDPMVDILAPMYPAVDTIIQMAQQPDPR-----PIIMCEYAHAMGNAVGNLKEYWEAIHRYPRLQGGFIWDWVDQGLRRRLP------DGQE--------FFAYGGDFGDYPNDGHFCLNGLTWPDRRPHPALWEVKKMYEPLQVEPVD---------------------------------------------------------LATGRLRLVNRFAFTNLSRF----RLSWRVEASGAVIAQGD----------LPGPDLPPGQSAG--------------------LAVPFPWPRLPSGG--ELFLSLHLSLPDDVPWAGAGHELAWAQFRLP-------GSPARVSARKPADLP---------PL----HLHTGETEIRVA------GRSTSWQFDRRSGRLAGWQ-------VGG----QALLTCGPRH----------NFWRAPTDNDMGMWGVHKLALQWYDAGLHCLAETAAETE---AHPLPDGGVAVTVRSVVAPPRPNEP-PRSARWQEFLAQLTDILYQFWNRAELAQLAAEMGVSLPDTAGPRVQSVRALVSACEQAGRVQPLFE------------AAYRRLLDASAEKTMETIKKRLEQFYGLSPQAFQAA---------------------------------FTAAYSARFDCTTRYHLSPDGAVRLEVQVVPADGLPPLPRVGLVMALPEQYRHFTWYGRGPHETYPDRKEGAMVGLYRGTVEEQFTPYIKPQENGSKTDVRWAELT----------------------DDAGRG------LRVEGDPLLNVSVSRYTDHDLAQARHLHDLRP-RAEAILR---LDHAQCGLG-NESCGPGVLPPYLLTAAEYRYALCLRPVGFW 1132