prot_A-nodosum_M_contig98.69.1 (polypeptide) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: D8LK54_ECTSI (Calpain catalytic domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK54_ECTSI)

HSP 1 Score: 865 bits (2235), Expect = 1.340e-305
Identity = 425/609 (69.79%), Postives = 481/609 (78.98%), Query Frame = 0

Query:    1 MPRLSAYEEAFNEDDEEEKGDTEEGDIIAKLKASHLGGLYEDGSFPASRSSLYRNAKRVPEYDVGVGAVKWLYPHEICQEPVYFSDDTISGGIDRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLDDETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAKPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTVHKRIWDRKQNDVVVNSQDFIFASTRAEREVT-GGVILNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGGPLRLVDEN-----NSKWCQNPQYFLKIP 603
            MPRLSAYEEAF+EDDEEE GDTEEGDIIA+L+AS  G LYED SFPA RSSLYRN K VPEYD  V  VKWLYP EICQEP YFSDD  SG + +GRLDDTWLLGA GV+A+HP  LIENLF SEP+DF +YGVYTCRFYKNGEWQ VVTDTRIPCA  SK+K D  L GQKVP  PTPMYG+ +NLNEQWVS+LEKAYAK+HGSYESI+GGSV EALVDLTGGCC+ +S   ER+KPM+EDGSLWET+  +V+ +YVVAC+Q                           ECSGFRLVR+RNPWS G+WKGDWSDASTLW+DY EVY+E+SSD   PW+RDSNDGT WM F+DFV LFT V  CR+FPD+ YRQYCIHGEW GKTAGGSAKP RRGSVS+LQ DGQ        +  DSLR ES  DIQ+DGD  WFNNPQYR+TADE++EVYISLMQQDR SA++LR+ +R+ FEV+QTKRHT HKRIW++KQ+DV+ +S +  F STR+EREVT   V L+PRYAYNIVPH +EQ REGKFTLRIFS K+V VEP+ ET T YL GSWD TSDRDTAGGPLRLVDEN     N KWCQNPQY L IP
Sbjct:    1 MPRLSAYEEAFDEDDEEEVGDTEEGDIIARLQASQRGALYEDVSFPAKRSSLYRNIKSVPEYDADVSEVKWLYPQEICQEPAYFSDDPGSGCLKKGRLDDTWLLGALGVLASHPGILIENLFASEPDDFVRYGVYTCRFYKNGEWQEVVTDTRIPCAHNSKNKGDDILLGQKVPGNPTPMYGHGLNLNEQWVSMLEKAYAKLHGSYESINGGSVGEALVDLTGGCCEKMSLTSERIKPMMEDGSLWETLRSHVSPEYVVACVQ---------------------------ECSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYSEVSSDTNTPWRRDSNDGTFWMVFSDFVGLFTKVYQCRVFPDEDYRQYCIHGEWSGKTAGGSAKPMRRGSVSRLQKDGQ-------DKMKDSLRRESVVDIQDDGDPYWFNNPQYRLTADETVEVYISLMQQDRRSANHLRENYRVGFEVVQTKRHTTHKRIWEQKQDDVIADSHENTFGSTRSEREVTRASVKLDPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPVTETFTSYLHGSWDRTSDRDTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIP 575          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A7S2D2G9_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2D2G9_9STRA)

HSP 1 Score: 695 bits (1793), Expect = 9.100e-234
Identity = 397/961 (41.31%), Postives = 552/961 (57.44%), Query Frame = 0

Query:   22 TEEGDIIAKLKASHLGGLYEDGSFPASRSSLYRNAKRVPEYDVGVGA-VKWLYPHEICQEPVYFSDDTISGGI-DRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACI-QLDPDRGACLDDETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAKPTRRGSVSQLQVDGQLS-----------ASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTVHKRIWDRKQNDVVVNSQDFIFASTRAEREVTGGVI-LNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGGPLRLVDE------NNSKWCQNPQYFLKIPQSAQYDS-VDV-------KIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRRNALGELVPTKASSLERVRERCRLRPNQETKSKEQQLLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWTMHSAGGSHIHPDWTKNPCFHLKLRIAGPAKVKISLSRP--ERDWKNKCLRDRVGCMMGFYLMVGTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTLRKER 951
            T E DIIA L  S       D +FP   +SLYRN   V +YD   G  ++WL   E  Q+P YF++ T S  +  RGRL+D WLLGA   +AAHP +LIENLF S P+DF +YGVYTCR YKNG W+ +V DTR+P A  S   A  K   Q       P+YG   N+NEQWVSLLEKA+AK HG+YE ++ G V+E LV+LTGG C+TI+ N + V+ MV+ G LW+ +  ++ + ++V C+ +   +    +D+ TGLL NHAY +  +K   GF  VR+R+PW  G W G+WSD S  W+D+ EV   +  DA   W+R S DGT WM FTDF  +FT + VCR+FPDD+YRQYCI   W GK+AG                                AS+  K      R ++   ++ DG+ +WFNNPQY+VTA E   ++ISLMQ+DR + + L+D  +I FEV+Q K    HKR W    +DVV ++          +REV G  I L P   YNIVPH+ +Q +E  + LRIFSP ++ ++ +P++H+V L G+W   ++RD+AGGPL +         +N KWC+NPQ+ L++P+     S VDV                                     +   GLV CK    +    RRR     R NALGE +PTK SSL+  R    L+ NQ+    E          P+RK+ ++  EW Q  DY  ++ + L+L ++     PHGL   L++PTLSE GAKG+F LEVHSD+ V V  +P+T SRT+A EWT  ++GGSH+H  W KNP +HL+L     +KVKISLSRP     WK +  +D VG M+GFYL+ G  P RD ++ ++++           G+P+  +P  P + VSTP  FVL+   ED++++I+PATFE GK GPFF+S+ AD +F+L+ ++
Sbjct:   16 TAEDDIIAMLHESGATNFC-DPAFPPDTTSLYRNKNFVADYDSRFGGNIRWLRCTEFAQQPDYFAEATESKHVLSRGRLNDVWLLGAMAAVAAHPDALIENLFGSAPDDFKRYGVYTCRLYKNGVWEEIVADTRVPTAEMSAPVAGLKPLAQA---SFCPIYGRCDNVNEQWVSLLEKAFAKYHGNYECLNHGDVAEGLVELTGGSCETIALNSKPVQEMVKSGKLWDMLTLHLEDQHMVTCVVEAGDEDDYSVDESTGLLNNHAYSVLMVKHVLGFEFVRVRSPWGGGVWTGEWSDDSPKWEDFPEVLENLKDDASVNWERRSYDGTFWMKFTDFCNIFTKIHVCRVFPDDEYRQYCIQSSWSGKSAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEYPKLVAKHARADA---VKADGNDTWFNNPQYQVTAAEPCNIHISLMQEDRRTDTRLKDNFQIGFEVVQMK-DPEHKRAWG-PNDDVVFDTASNPLNGDLLQREVCGSNIHLEPGTTYNIVPHAAQQGKESPYILRIFSPVDLEIKEVPQSHSVTLPGAWQRVTERDSAGGPLLVPGSGGKSCASNPKWCENPQFVLRMPKHVPTSSSVDVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLKVGLVACKIPAPEDERSRRRVPGERRINALGEEMPTKESSLKNPRSHF-LQQNQDASEVE---------MPDRKMVIDAKEWYQTTDYKRSDVAMLMLPNVSVNWAPHGL---LLIPTLSEIGAKGQFVLEVHSDYPVVVESLPDTKSRTLAGEWTESNSGGSHMHQSWKKNPLYHLRLLGNKRSKVKISLSRPAGNASWKAQTGKDSVGSMIGFYLVQGPAPTRDAAE-VYHD-----------GKPWSETPFVPMNSVSTPSNFVLEPL-EDEVYSIIPATFEAGKKGPFFLSVVADCDFSLQLQK 941          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A836CKR5_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKR5_9STRA)

HSP 1 Score: 621 bits (1602), Expect = 1.040e-208
Identity = 334/726 (46.01%), Postives = 434/726 (59.78%), Query Frame = 0

Query:    1 MPRLSAYEEAFNEDDEEEKGDTEEGDIIAKLKASHLGGLYEDGSFPASRSSLYRNAKRVPEYDVGVGAVKWLYPHEICQEPVYFSDDTI-SGGIDRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPT----PMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLDDETGLLLNHAYGLEDMKECSGFRLVRLRNPW-SQGQWKGDWSDASTLWDDYLEVYTEMSSDAKK--------------------PWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQ-YRQYCIHGEWFGKTAGGSAKPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRH---------TVHKRIWDRKQNDVVVNSQDFIFASTRAEREVT-GGVILNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGGPLRLVDE--------------------NNSKWCQNPQYFLKIPQSAQYDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAAS 669
            M +L A +++ +E+D+EE G++EE D+I  L+ S  G  YED SFPAS+S+LYR++  VPEYD+GVG +KW+ PHE+ ++P YF+D +  +G + +GRLDD WLLGA   +AAHP +LIENLF SEP DF  YG+YTCRFY NGEWQ +VTDTR+PC             G + P G      P+YG  V++NE WV LLEKAYAK+HG+YE++ GGSV EALVDLTGG    I   + RV+ MV DG LW+ + RY+   Y++A                G+ LN A  L  ++E SGFRLVR+R+PW +   W GDWSDAS  W+DY EV   +S+                        PW+RD  DGT WM + DFV  FT V VCR+FPD   +RQYC+HGEW G   G +A P           D             +SLR  +  D+Q DGD  WFNNPQYR+T D+  EV ISLMQQDR SASYLR+ H + FEV++ +R            H R+W+R+  DVV +S    FA  R EREV   GV L+PR AY +VP   E  REGKFTLR+F+  +V +EP+PETHT+YLRG+WD TS+RDTAGGPLRL +E                    NN KWCQNPQ++L+IP  A YDSVDVK++L++ +DG          GKP ++             +   I G + R R +A+
Sbjct:    1 MGKLEALKDSMDEEDDEEVGESEEADVIENLRESGSGKRYEDTSFPASKSALYRSSTIVPEYDMGVGEIKWMRPHELARDPTYFNDGSAGAGNVVKGRLDDAWLLGAMAAVAAHPSNLIENLFTSEPGDFNNYGIYTCRFYINGEWQEIVTDTRLPCTPLPP--------GAEPPAGAACGHVPLYGRGVDINEFWVPLLEKAYAKLHGTYEALSGGSVGEALVDLTGGSTARICLEEARVEAMVGDGRLWDRLRRYLRWQYILAA---------------GVPLNAACSLVGLREVSGFRLVRVRSPWGAAAAWTGDWSDASPRWEDYPEVLAAISAGXXXXXXXXXXXXXXXXVDAVGGGPWRRDGGDGTFWMAWEDFVARFTHVYVCRVFPDGAGFRQYCVHGEWAGDAMGLAAAPAXXXXXXXXXXDAVA----------ESLRRTTMVDVQPDGDPFWFNNPQYRLTVDKPTEVLISLMQQDRRSASYLRENHGVGFEVLRARRRGGSXXXXXAAPHARVWERRAEDVVTDSARASFAPARPEREVAKAGVTLDPRLAYVVVPTCAELGREGKFTLRVFAQSDVVMEPVPETHTLYLRGTWDRTSERDTAGGPLRLPEEAPSGSXXXXXXXXKEGRGVRNNPKWCQNPQFWLRIPDGAAYDSVDVKLVLRRVADGPGGXXXXXRGGKPPKVRARAGVARQHCAAEHHLIAGFLCRARASAA 693          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A7S1U0F0_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U0F0_9STRA)

HSP 1 Score: 577 bits (1488), Expect = 1.250e-187
Identity = 368/1020 (36.08%), Postives = 527/1020 (51.67%), Query Frame = 0

Query:   22 TEEGDIIAKLKASHLGGLYEDGS--FPASRSSLYRNAKRVPEYDVGVGAVKWLYPHEICQEPVYFSDDTISGGIDRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDP-----------DRGACL---DDETGLLLNHAYGLEDMKECSGFR-LVRLRNPWS-QGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDS-NDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAKPTRR-----------------------GSVSQLQVDGQL--SASKERKRNIDSLRN--ESGTDIQNDGDLSWFNNPQYRV-TADESLEVYISLMQ-QDRSSASYLRDYHRIAFEV--IQTKRHTVHKRIWDRKQNDVVVNSQDFIFASTRAEREVT-GGVILNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGGPLRLVDEN-----------------------------------NSKWCQNPQYFLKIPQSAQ---YDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRR-NALGELVPTKASSLERVRERCRLRPNQETKSKEQQLLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWTMHSAGGSHIHPD-WTKNPCFHLKLRIAGPAKVKISLSRPERDWKNKCLRDRVGCMMGFYLMVGTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTLRKE 950
            TEE +IIA+L+      L  DG+  F     +LYRN  +VP YD   G  +WL P +  + P YF        +  GRLDD W LGA   +A+HP  L+E LF S  +DF +YGVYTCRFY NGEW+ V+ DT++P   K  +        ++ P G  P+YG+  +  E WVS +EKAYAK++GSYE+++ GS +EALVDLTGG C  +    +  K +   G LW+ +  ++ ND  + C Q++P           D    L   D  TGLL N  Y +  ++E  G   LV +RNPW  +G W+GDW + S +W+DY +V   ++S    PW  D  N+G  WM F DF   FTT+ VCR+F DD Y QYC+ G+W GKTA G  +   R                       G +S    DG    SA+ +++   +SL    E    +  DG+  WFNNPQ+RV T      +Y+SL Q Q R + +  R    I F +  I ++   V  RIW+ K  DV+ +S   + AS  A  EV    V L P   Y IVPH+ ++ REG+F LR+F    + +  + ET +VYL G+W+ T ++D+ GGP+ +V+E                                    N KWCQNPQY+++  +++      S  +K ++++       R +E    KPR  +   +   G+V  K  G    V  RR   + RR NALG+ +PTK S+L+R         N+     E Q+       PE    +   +W +M  Y + E + L L ++       GL   +V P LS +G KG + LEVHS   + +  + + A +T+A EW   SAGGSHIH D W KNP F L+L   GPA+VK+ LSRPE  W  K LR+ VG MMGFY+  G +PNR             GA     G+P+  S   P+ DV TP  F+L+   +DD +TI+PAT++PG  GPF ++++++  F+LR+E
Sbjct:    5 TEE-EIIAQLE------LQADGTSIFEDDWKALYRNPSQVPAYDEHGGDFRWLRPAQYLRRPEYFHWVGDRVQLQAGRLDDAWFLGALAAVASHPAGLVEALFGSNADDFKRYGVYTCRFYLNGEWREVICDTKLPVEDKPVNPFADMEAAEEGP-GMAPIYGHSAHPEELWVSFIEKAYAKLNGSYENLNEGSPAEALVDLTGGSCVELDMASDTAKQLAGSGELWDLLLAHI-NDGGILCGQVEPPNASEGKEELADADPALVEPDPVTGLLPNRLYTVIALREVPGAPPLVLVRNPWGIEGDWEGDWGEGSAMWEDYPDVEEALASGNTTPWTTDGENNGLFWMPFVDFCENFTTLFVCRIFDDDAYYQYCVEGDWAGKTAAGPPRRPLRAAQGVLDFNAVYPQGKKNEKKGAGGISPKASDGAAAPSANGQKEAYAESLARHFEGSKMLCEDGEPFWFNNPQFRVVTGSRPCRIYVSLQQRQGRRAGAAKRVGPSIGFHLLKIDSEAEGVLGRIWELK--DVIADSAQDLMASKEALGEVVCQDVTLEPDTKYVIVPHAGQKNREGRFCLRVFCRSALDIASVTETSSVYLPGAWERTPEQDSCGGPVLVVEEESKPANKAGMPLTPPPTEPEEEGAQPETVTLLKMKQNPKWCQNPQYWVRFAETSDDPLAQSATIKFVVRRTD-----RRQEGTRRKPR--AGADDVQVGVVAVKVRGRATSVTSRRRQQNKRRVNALGKPLPTKESTLKRRTV------NRNATGDEMQV-----EAPETLKCINAADWHRMNLYENVETACLTLTNVTSASFTDGL---IVSPCLSSRGTKGTYVLEVHSSLPLRIEGLTDGADKTLAGEWAEGSAGGSHIHTDSWKKNPKFQLRLNSTGPAQVKVRLSRPEDYWAPKMLRNAVGAMMGFYIYQGQRPNR-------------GAVTLVNGKPWSDSAFVPSLDVCTPQNFILEPLPDDDFYTIMPATYDPGNHGPFHLTVTSEAPFSLRRE 979          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A1V9ZBZ2_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZBZ2_9STRA)

HSP 1 Score: 522 bits (1344), Expect = 1.620e-167
Identity = 329/956 (34.41%), Postives = 492/956 (51.46%), Query Frame = 0

Query:   20 GDTEEGDIIAKL-KASHLGGLYEDGSFPASRSSLYRNAKRVPEYDVGVGA------VKWLYPHEICQEPVYFSDDTISGGIDRGR-LDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTP-MYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISF-NDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLD-DETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAKPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTVH-KRIWDRKQNDVVVNSQDF---IFASTRAEREVT-GGVILNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSW-----DHTSDRDTAGGPLRL----VDENNSKWCQNPQYFLKI-PQSAQYDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRRNALGELVPTKASSLERVRERCRLRPNQETKSKEQQLLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWT--MHSAGGSHIHPDWTKNPCFHLKLRIAGPAKVKISLSRPERDWKNKCLRDRVGCMMGFYLMVGTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTL 947
            G T E ++IA L +   +  +YED  F A   SLY  +  +PEY            V W  P+++C +P YF   +  G +  G  L+D WLLG    +A HP +LIENLF+S+  DF  YG+YTC+FYK+ +W  VVTDTR+P + +     D ++    V D P   +YG  +N NE ++ LLEKAYAK HG+YE++H GS+ EA +D TGG  K +   +D   K ++E GSLW  + +++     V   QL     A  D    G+L N  Y +  +KE    R ++LRN W +G WKGDW +  + W+D L+V   + +D    + R  NDGT WM + DF+  F  + + R+FP   Y QYC+ GEW G+ A G   PT+  S  + Q     S S+                +Q D + +WF NPQYR+  +E   V ISL+Q+D     +  D   I F VIQ K+  ++   +W+  +  +V  +  +     +S   ERE++ G ++L P  AY IVP++  Q  E +F LR FSPK   ++ +   +T+ + G W     +  S+  TAGGPL L        N  WCQNPQY L+  P++    S+ VKI++K+ S  + ++ + ++A K R     H     LV      +  GV ++      + + LGE            + +C++                    P+RK+ V+  E C +  Y S   +TL L+ +    +  GL   ++VPTL E+  +G F +E+HSD  V V EI    ++TV  EW+    + GG+H++PDW KNP F+L L+   PA V I L R E +W+ KC +D VG MMGFYL  G K  RD S       +T+       GR +  +   P H + +P   +    +E   + I+P T+EP +TG F +S+SAD +FTL
Sbjct:    7 GITLERNVIAMLSECGDIPPMYEDPDFAAKPVSLYVKSDVIPEYATSAKKGDDGTIVHWYRPNQVCTDPDYFKSTSGCGTLREGTGLNDCWLLGVLAALALHPDNLIENLFVSQMHDFKTYGIYTCQFYKDCQWLEVVTDTRLPYSQELLEVNDCRVANSNVMDQPGHWLYGSSLNKNEVFIPLLEKAYAKFHGNYETLHNGSIVEAFIDCTGGSVKVVDLASDSTRKELIETGSLWHKLVKHLNQFKSVLSCQLKMASMAYNDVTSAGILKNRLYAILHLKELGSLRFLKLRNVWQRGNWKGDWCNDDSKWEDNLQVEAALRADPACDFNRTKNDGTFWMVWEDFIEAFNELFITRIFPTSVY-QYCVRGEWIGQGAAGP--PTKINSEDKAQE----STSRR-------------WTVQGDSEPAWFRNPQYRIVVEEKTTVLISLLQRD--FRVFGGDNFAINFGVIQVKKKALNASMVWEYDKEHIVAEAHSYEPGTESSQHPEREISKGNIVLEPDRAYIIVPYTDHQGVEMEFFLRFFSPKSCHIDALRPLNTLVVHGKWRSSDENGNSNTSTAGGPLCLNLSTTGLENLAWCQNPQYTLRANPKNDNQRSITVKIVVKQTSYKATSKAR-RDASKER----AHLLGITLVRPDVPEVDTGVHKK--TKHDKTDFLGEN-----------KGKCKIG-----------------AIPDRKLLVKADEMCTLSSYSSPSIATLFLQKINPEWLAQGL---IIVPTLGERMTEGTFEMEIHSDDPVTVDEIRSDTTKTVFGEWSEDKSTTGGAHLNPDWKKNPKFYLTLQCVRPAAVVIHLYRAEHEWRTKCKKDSVGTMMGFYLFQGNKIQRDSSS------VTV------DGRSWTETDFVPMHHIQSPQLSLPALFNES--YCIMPTTWEPNRTGRFLLSVSADCDFTL 888          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A8J2SDL8_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SDL8_9STRA)

HSP 1 Score: 514 bits (1325), Expect = 2.730e-164
Identity = 347/978 (35.48%), Postives = 505/978 (51.64%), Query Frame = 0

Query:   23 EEGDIIAKLKASHLGG-LYEDGSFPASRSSLYRNAKRVPEYDVGVGA-VKWLYPHEICQEPVYFSDDTI--SGGIDRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGA--------CLDDETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQ--GQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQR-----DSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAKPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRH-TVHKRIWDRKQNDVVVNSQDFIFASTRAEREVT-GGVILNPRYAYNIVPHSI-EQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGG-PLRLVDEN---NSKWCQNPQYFLKIPQ------SAQYDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRRNALGELVPTKASSLERVRERCRL--------RPNQETKSKEQQLLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWTMHSAGGSHIHPDWTKNPCFHLKLRIAGPA---KVKISLSRPERDWKNKCLRDRVGCMMGFYLMV-----GTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTLRKERG 952
            EE  +I +L+ S      Y D  F    ++LYR+ + VP YD  +   + WL P E+C++P YF D  I  SG +  GR  D WLLGA   +  HP  LIENLF S+P+DF ++GVYTCRFY+ G W  V  DTR+P  +       +     + P GP  +YG  V+  EQWV LLEKAYAK+ GSYE++  GSV+EALVDLTGG  + I  N++  K   +  +L+E    Y+ N +V+ C+  DP                  GLL  HAYG+    +    +LVRLRNPWS   G WKG W+D+S+ W+D+ EV  E    A   W R     D  DG+ +M F+DF   F T+ +CRLF D QY+QY +   W GKTA G   P     VS      +L A+K + R               D D  WFNNPQ+RV        +ISL+Q    S         +  EVI+ K+   +  R+W+R   DV  +++     +    REV+   V+L P Y Y +V  +  E+ RE  F LR+FSP+E+ ++P+ E  + YL GSW+  ++ D+AGG P     +    N  + Q+PQYFL +P+       A    V+V ++L++      T  K Q A              GL V +    +  + ++     P+ NALGE    K S+L++ R +           +   +   + +  +   P    +KIG+ P EW ++  + +   + L L +L + + PHGL   ++ P LSE+G  G++ LE+H+D    +  I  + S+T+A EWT   AGGSH+ P W+ NP +  ++R A P    +V++ L+RP   W +    D VG MMGFY+       GT P+ D    I +    +   +  +  PY  SP TP H V TP  F L  +  +D F IVPAT+ PG+TGPFF+S+++DV+FTL   +G
Sbjct:    7 EEDAVIQQLRESRTEDEQYFDVHFGGLPAALYRDEEDVPPYDSNLDEPIVWLRPGELCRDPQYFVDGAIGVSGCVVEGRNGDGWLLGAMAALWGHPEYLIENLFGSDPDDFQEWGVYTCRFYREGSWVEVCADTRMPALS------GFTAAEGEEPTGPQCLYGRCVDPREQWVQLLEKAYAKVLGSYEALSKGSVTEALVDLTGGSGEEILLNEDSAK---KTSTLFEECQDYLENKHVL-CVTADPANNGQGGVPSPDLKQTALGLLPGHAYGVMQCIQVEDKKLVRLRNPWSGRGGTWKGPWADSSSQWEDFPEVLDECLKQASG-WTREEDGIDQTDGSFFMEFSDFCSKFDTLHLCRLFDDKQYKQYKVLSSWAGKTAAG---PDEGVPVSTDTGFAKLIAAKAKPRT--------------DKDACWFNNPQFRVEVKHKSTTHISLLQGPPKSGERFE----LCLEVIREKKTPALPGRVWER--GDVYRSTE----VNQPVMREVSLSNVVLEPGYTYCVVARAATERGREAPFALRVFSPRELVLQPLDECESSYLPGSWNGAAEVDSAGGAPFHFEKDQLIPNPTFRQSPQYFLNLPEPPPDHPKATTGEVNVTLVLRRTDP---TPDKAQKA------------QIGLCVFRGPAPEVVLGKKHKKTGPKVNALGEKPKGKPSTLKKKRRQAAQLLDGLAGPQHGWDNDLRSEDSITTAP----KKIGLYPSEWHRLSSFGNETVAVLHLPTLVRSKFPHGL---VITPCLSERGISGQYVLELHADCPTTLEMISASKSKTLAGEWTARHAGGSHLQPSWSTNPIY--RVRFADPTARPRVRVDLTRPAEYWNSS---DVVGRMMGFYIHRADGPNGTGPSLDPDHCIMHVDPNL---KDVEPEPYTQSPFTPLHTVQTPQAFRLDKS--EDPFLIVPATYAPGQTGPFFLSVTSDVDFTLTAAQG 914          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A7S1C892_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C892_9STRA)

HSP 1 Score: 510 bits (1313), Expect = 5.630e-163
Identity = 323/938 (34.43%), Postives = 473/938 (50.43%), Query Frame = 0

Query:   23 EEGDIIAKLKASHLGGLYEDGSFPASRSSLYRNAKRVPEYD-----VGVGAVKWLYPHEICQEPVYFSDDTISGGIDRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLDDETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAK--PTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTVHKRIWDRKQNDVVVNSQDFIFASTRAEREVTGGVI-LNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGGPLRLVDENNSKWCQNPQYFLKIPQSAQYDS-VDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRRNALGELVPTKASSLERVRERCRLRPNQETKSKEQQ------------LLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKS----LRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWTMHSAGGSHIHPDWTKNPCFHLKLRIAGPAK-VKISLSRPERDWKNKCLRDRVGCMMGFYLMVGTKPN----RDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPG 930
            EE +I+A L+    G LYED SF  + +SLY N   +PEY      +  G   WL PHE  + P  F   T +G + +G+L D WLLG+   +AAHP  LI+NL +S+ + +   GV +C+F+KNGEW  V TDT IP        AD        P    P+YG   +++E WV  +EK YAK+HG+YE ++GGSV+EA+VD TGG  +     DE VK  +  G LW  M    + ++++ C       G   D  +G+L+NHAY +  +KE    + V++RNPW  G+W+GDWSD    WD + +V + +  D    + R   DGT WM + DF + F  + VCR+F ++ Y QY +HG W G +A GS K    R G+  +   D      K+ KR               DGD  WF+NPQYRVT  +  +  ISL Q DR       +   I F V++  +++  +R+W+   +D+V +S    FA+  A+REV    +  +P+Y+Y IVPH++++  E  F LRIFS  E+ V+ +P+ + + L  +W     RD AGGP R+    N  +C NPQ+++ +P  +       +K++L +          ++NAG+  +   T N + GL V +        PRRR     R NA+GE +P+K SSL R        P  +T + E +              P  P  P+RK+ V   E+     Y   + +T LLK     LR G        LLV P +   G +GEFTL++ S+  + + E+ +  SRTV   W+  +A G H+HP+W +NP F + L+    A   KI+LSRPE  WK     D VGCM+GFYL+   +       D  K  F E   +                 P H  S           E  +F ++PATFEPG
Sbjct:   37 EEDEILATLRD---GELYEDRSFAPNDASLYHNLSVIPEYANVEPRLSKGPHPWLRPHEFAKAPELFKAGTEAGDVVQGQLGDCWLLGSLASVAAHPDDLIDNLIVSDDDTWKTSGVVSCQFFKNGEWVTVTTDTMIPYG----DPAD--------PAFNMPIYGRCRDISELWVPFIEKCYAKLHGTYEGLNGGSVAEAMVDFTGGVAEKFLLTDEHVKARIASGELWRDMMELKSGNHLLGCSMSRFGGGFETDAGSGILINHAYSILHLKEVGSLKFVKIRNPWGHGEWRGDWSDNWPKWDQHPDVESALMDDPACKFNRSDADGTFWMIWEDFTKQFNKIYVCRVFGEE-YNQYVVHGRWEGLSAAGSHKVMEDRDGAGEEKDAD---ETEKQSKRQ--------------DGDAQWFHNPQYRVTCTKPAKACISLTQSDRMLTGRAENV-AIRFVVLRCPKNSK-QRVWEEDPSDIVADSNASNFANMFAQREVVHPEVEFDPKYSYVIVPHTMQRGMELGFNLRIFSEAELAVDAVPDPYGIELSSAW----IRDGAGGPRRVDGRANPAFCLNPQFWITLPPGSDTKKKTTLKLVLTRT---------DRNAGRDDKSDGTKNRV-GLTVTRVQPPPPDNPRRRRPGQVRTNAMGEPLPSKESSLRR--------PLGDTAASEAKGGAGGMGATAAAAEPHDP--PKRKLDVTNEEYTLTSYYTDAKVATTLLKVPADWLRDG--------LLVHPAMKLPGREGEFTLQLFSETQLMMQELSQGHSRTVTGSWSDKNAMGCHLHPEWKRNPAFQIVLQDRPVAMDCKITLSRPEDRWKAMARADSVGCMIGFYLVTCDRERDSKFNDGRKDFFQETSFV-----------------PMHQQSLTTSLRFDLLPEGKVFLVIPATFEPG 890          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A024UDH4_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UDH4_9STRA)

HSP 1 Score: 507 bits (1306), Expect = 1.450e-161
Identity = 332/970 (34.23%), Postives = 487/970 (50.21%), Query Frame = 0

Query:   20 GDTEEGDIIAKL-KASHLGGLYEDGSFPASRSSLYRNAKRVPEY-------------DVGVGAVKWLYPHEICQEPVYFSDDTISGGIDRGR-LDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLD-DETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSA-KPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTV-HKRIWDRKQNDVVVNSQDFIF--ASTRA---------EREVT-GGVILNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSD-RDTAGGPL---RLVDENNSKWCQNPQYFLKIPQSAQYDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRR-NALGE--LVPTKASSLERVRERCRLRPNQETKSKEQQLLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWT--MHSAGGSHIHPDWTKNPCFHLKLRIAGPAKVKISLSRPERDWKNKCLRDRVGCMMGFYLMVGTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTLRKE 950
            G T E ++IA L +   +  +YED  F A   SLY N  + PEY             D    A+ W  P ++  +P YF      G +  G  L+D+WLLG F  +A HP +LIENLF+S   DF  +G+YTC+FYK+ +W  VVTDTR+P +   +   + K     +  G   +YG  VN +E +V LL KAYAK HG+YE +H GS+ EA VD TGG  K I   D+  + ++E G LW  + ++V    VV C QL     A  +   TG+L NH Y ++ +KE    + V+L+N W +G WKGDWS+  + W+D L+V   + +D    + R   DGT WM + DFV  F  + + R FP   ++QYC+ GEW G+ A G   KP      S        SA K                ++ D + SWF NPQYR+   E   V +SL+Q+D     +  D   I F +++ K+  +    +W+  +  VV     +    A+T A         ERE++ G V+L P  AY  +P++     E +F  R+FSPK V+++ +   +T+ ++G W    +    AGGPL         N  WCQNPQY L+    A    VD+K++LK+    +  +G  ++  K +        L GL + K    +           P + N LGE  L PTK    + V          +TK+ E   LP       RK+ V+  E+C + DY S   +++ L+ +    +  GL   LVVP+L +   +G + +EVHSD  + + EIP    +T+A EW   +++AGGSH+ PDW KNP F+L L+   P  V I+L R E +W+ KC +D VG MMGFYL  GTK  RD S  +              G+ +  +   P H VS+P    L     +  + I+P T+EP K G F +S+ +D EFTL+ E
Sbjct:    7 GITLERNVIAMLSECGDIPPMYEDPDFAAKPISLYLNPDKPPEYATSSKRPTANGGADEAASAIAWYRPGQVTADPDYFKCTAGCGVLREGTGLNDSWLLGVFAALALHPDNLIENLFVSPMHDFKTFGIYTCQFYKDCQWLEVVTDTRLPYSQPLEEVNEPKTGSAVMRPGHW-LYGSSVNKSELFVPLLMKAYAKFHGNYEVLHNGSILEAFVDCTGGSVKKIDLADDASRKLIETGQLWHKVVKHVHYKSVVTC-QLKMASMAYNEVTGTGILKNHLYVVQYVKELGPLKFVKLKNVWQKGLWKGDWSNDDSKWEDNLQVEAALRADPLCEFNRTKADGTFWMMWEDFVEAFNELYIVRNFPST-FQQYCVRGEWVGQAAAGPPMKPPPSVETSSASAPSAPSARKWL--------------VEPDSEPSWFLNPQYRLVVTEKTSVVLSLLQRD--FRVFGGDNFGINFVLLEVKKRALASSMVWEFDKTAVVAEGHSYPSGAAATAALGDKSAGHPEREISKGNVVLEPDVAYIFIPYTDHGGVEMEFFFRVFSPKPVQIDSLTPLNTLIVQGRWRVDDEGHSNAGGPLVQNLTTGMENLTWCQNPQYVLR--SFAASKPVDIKLVLKRTGIKATAKGHRRDHQKDK------GQLIGLAIVKPDVDESAAQATCKKKHPEKTNFLGEPLLSPTKPRRAQSV---------VQTKTAENATLPP------RKLLVKADEYCVLSDYSSAHVASIFLRKVPPEWLAKGL---LVVPSLGDARGEGAYDVEVHSDDVITMDEIPNEMVQTIAGEWNDKVNTAGGSHLCPDWKKNPKFYLTLKCVRPVSVTINLYRSEYEWRGKCKKDSVGAMMGFYLFQGTKGTRDTSTVV------------VDGKTWTETDFVPLHHVSSPKDLTLPPVFNES-YVIMPTTWEPNKCGRFLLSVESDCEFTLQGE 918          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: A0A6G0XCD7_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XCD7_9STRA)

HSP 1 Score: 497 bits (1279), Expect = 6.710e-158
Identity = 316/961 (32.88%), Postives = 483/961 (50.26%), Query Frame = 0

Query:   20 GDTEEGDIIAKL-KASHLGGLYEDGSFPASRSSLYRNAKRVPEYDVGV------GAVKWLYPHEICQEPVYFSDDTISGGIDRGR-LDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLDDETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGG-------SAKPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTDIQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTVH-KRIWDRKQNDVVVNSQDFIFASTRA---------EREVT-GGVILNPRYAYNIVPHSIEQKREGKFTLRIFSPKEVRVEPIPETHTVYLRGSW--DHTSDRDTAGGPL--RLVDENNSKWCQNPQYFLKIPQSAQYDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRRNALGELVPTKASSLERVRERCRLRPNQETKSKEQQLLPIRPVFPERKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWT--MHSAGGSHIHPDWTKNPCFHLKLRIAGPAKVKISLSRPERDWKNKCLRDRVGCMMGFYLMVGTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTLR 948
            G T E ++IA L +   +  +YED  F A   SLY++ +++PEY          G + W  P ++  +P YF      G +  G  L+DTWLLG F  +A HP +LIENLF+S   DF  YG+YTC+FYK+ +W  VV DTR+P + + +   +   +G   P     +YG  VN +E +V LL KAYAK HG+YE +H GS+ EA VD TGG  K I+   +    ++E G LW  + ++V    V+ C              +G+L NH Y ++ +KE    + V+L+N W +G WKGDWS+  + W+D L+V   + SD    + R   DGT WM + DFV++F  + + R FP   + QYC+ GEW G+ A G        A P    + ++                           I  + + +WF NPQYR+   E   V ISL+Q+D     +  D   I F ++Q K+ ++H  ++W+  Q+ VV  +        +A         EREV+ G +IL P  AY +VP++     E +F LR+FSPK V+++ +   +TV L+G W          AGGPL   L    N  WCQNPQY L+    +    VD+K++LK+    +  +G+  +         ++    GL + +        P  +   S + N LGE   TK  + E    +                       P RK+ V+  E+C + DY + + ++++L+ +    +  GL   LVVP+L E    G + LEVHSD  + + E+P   ++T+A EW+    +AGGSH+ PDW KNP  +L L+    A+VKI L R E +W++KC +D VG MMGFYL  GTK NR+ S  +              G+ +  +   P H ++  +   L     +  + I+PAT+EP  +G F ++++AD EFTL+
Sbjct:    7 GITLERNVIAMLSECGEVPPMYEDPDFAAKAISLYQDPEKLPEYATSAKRAVDDGEIHWYRPGQVTADPDYFKCTFGCGTLREGTGLNDTWLLGVFAALALHPDNLIENLFVSPMHDFKTYGIYTCQFYKDCQWLEVVADTRLPYSQQLEDVNEPGTKGVARPGHW--LYGSSVNKSEVFVPLLMKAYAKFHGNYEVLHNGSILEAFVDCTGGSVKMINMMLDESHKLIETGQLWYKLVKHVHYKSVITCQLKMASMSYNEVTGSGILKNHLYIIQHVKELGPLKFVKLKNVWQKGLWKGDWSNDDSKWEDNLQVEAALRSDPLCEFNRTKIDGTFWMIWEDFVQVFNEIYIVRNFPQT-FNQYCVRGEWVGQAAAGPPLKQHPEASPPAPPTTTRKWF------------------------IAPESEPAWFLNPQYRLVVTEKTSVVISLLQRD--FRVFGGDNFGINFVLLQVKKRSLHASKVWEFDQDAVVAEAHSLPQQQQQANGTDKTHHPEREVSKGNIILEPNEAYILVPYTDHVGVEMEFFLRVFSPKPVQIDALRPLNTVLLQGKWRVSEEGSATNAGGPLCHSLAAAENLHWCQNPQYLLRSMDDSG-KPVDMKLVLKRTGYKATAKGRRDH------QKDSNAQCIGLTIVRPDVQVDASPVVKKKPSEKANFLGE---TKHKAKEGGGNKA---------------------LPSRKLLVKADEYCCLSDYANPQVASVMLQKIAPEWLDRGL---LVVPSLGEGRIDGAYELEVHSDASLTLEELPNGLAQTIAGEWSDKKGTAGGSHLCPDWKKNPKVYLTLKSVRAAEVKIDLYRSEYEWRSKCKKDSVGSMMGFYLFQGTKLNRESSTVV------------VDGKTWTETDFVPLHHIAASL--TLPPVFNES-YVIMPATWEPNHSGRFLVAVTADSEFTLQ 889          

BLAST of mRNA_A-nodosum_M_contig98.69.1 vs. uniprot
Match: J9ISJ7_9SPIT (Calpain, putative n=2 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9ISJ7_9SPIT)

HSP 1 Score: 459 bits (1182), Expect = 3.520e-143
Identity = 302/938 (32.20%), Postives = 471/938 (50.21%), Query Frame = 0

Query:   39 LYEDGSFPASRSSLYRNAKRVPEYDVGVGAVKWLYPHEIC-QEPVYFSDDTISGGIDRGRLDDTWLLGAFGVIAAHPCSLIENLFISEPEDFGQYGVYTCRFYKNGEWQGVVTDTRIPCATKSKSKADYKLRGQKVPDGPTPMYGYIVNLNEQWVSLLEKAYAKIHGSYESIHGGSVSEALVDLTGGCCKTISFNDERVKPMVEDGSLWETMNRYVTNDYVVACIQLDPDRGACLDD---ETGLLLNHAYGLEDMKECSGFRLVRLRNPWSQGQWKGDWSDASTLWDDYLEVYTEMSSDAKKPWQRDSNDGTSWMTFTDFVRLFTTVCVCRLFPDDQYRQYCIHGEWFGKTAGGSAKPTRRGSVSQLQVDGQLSASKERKRNIDSLRNESGTD--IQNDGDLSWFNNPQYRVTADESLEVYISLMQQDRSSASYLRDYHRIAFEVIQTKRHTVHKRIWDRKQNDVVVNSQDFIFASTRAEREVTGGVILNPRYA-----YNIVPHS---IEQKREGK-FTLRIFSPKEVRVEPIPETHTVYLRGSWDHTSDRDTAGGP-LRLVDENNSKWCQNPQYFLKIPQSAQYDSVDVKIILKKQSDGSHTRGKEQNAGKPRRMSYTHNALTGLVVCKASGIQGGVPRRRHAASPRRNALGELVPTKASSLERVRERCRLRPNQETKSKEQQLLPIRPVFPE---RKIGVEPLEWCQMPDYVSTEASTLLLKSLRKGQMPHGLHGLLVVPTLSEKGAKGEFTLEVHSDHGVEVGEIPETASRTVASEWTMHSAGGSHIHPD----------WTKNPCFHLKLRIAGPAKVKISLSRPERDWKNKCLRDRVGCMMGFYLMVGTKPNRDQSKTIFYEPITIGARRRAQGRPYEGSPMTPNHDVSTPIGFVLQSTSEDDIFTIVPATFEPGKTGPFFISISADVEFTL 947
            L+ D  FPA+  SLY++   +P+YD  +  V+W  P EI   EPV   D T  G + +G L D WLLG+F +++ HP  L++NL + +  +FG + V+  +F+KNG+WQ V+ DTRIP    SK                TP+YG+  + NE WV LLEKAYAK+HG+YE+++GGS++EALVDLTGG  +  +      K  +E G  W+ + +Y  + Y++ C     D G   +D    TG+L NHAYG+ D+++  G +L+R+RNPW  G+W G ++D    WDD+  +  ++    K       +DGT WM + D+   +  + +C++FP   + QY I GEW G +AGG+  P          VD                R+E   D  ++ D +  WFNNPQYR++  +  +V ISLMQ+D   +   R Y  + F V++ K  +  +R+W+  ++DVV+ + D +      +RE+T    L P +      Y IVP++   + +K E + F LRIF+ + + V  +P T     +G W     ++TAGG  +    + N  WC+NPQYFL I +        +K+ILKK+  G   RG                   G+ V KA       P       P ++  G  +   ASS+ +  +      N  TK K   +    P   E   RK+ +   EW +   Y S + + L     +  Q P      +++P++ ++    +FTL + S   VEV ++ ++ +  ++ +W+  SAGG H++            WT NP FH++ +   P +VKI+LSRPE+ WK +   + VGCM+GFY+  G   N+  +K      ++                  P ++VS      L      D + ++P T+EPGK GPF IS S DVEFTL
Sbjct:  108 LFGDSEFPATDQSLYKDPTNIPDYDQNMPLVEWRRPQEIAPDEPVMIKDGTSPGDVKQGNLGDCWLLGSFLILSTHP-DLLQNLIVHDGIEFG-FAVF--QFFKNGKWQYVIVDTRIPYNQSSK----------------TPLYGHCADPNEFWVPLLEKAYAKLHGTYEAMNGGSMAEALVDLTGGASEKYNLRAPETKENIESGQYWKDLKKYFQSGYLIGCANSIKDDGGKQEDGMGPTGILFNHAYGVMDVRDIDGLQLIRIRNPWGHGEWTGKFADEDEAWDDHKGLKDKLQYVFK-------DDGTWWMRYEDWCAHYNKMYICKIFPSS-WSQYSITGEWKGNSAGGAYPP---------MVD----------------RDEETKDARVEQDSNDRWFNNPQYRISVSKKTQVIISLMQEDEKISK--RPYIPVNFLVVRVK--SKRERLWEIDKDDVVLEAADGL--QRFGQREITKTTTLLPIHDKKNVHYIIVPNTEDGVNKKEEERPFFLRIFASEHLEVAQLPPTIEQQFQGKW----AQNTAGGKRVTETGKENQFWCRNPQYFLNITKP-----THIKVILKKKG-GRRVRG----------------VPIGITVVKAFPPTN--PPTAKIIKPGKDQGGNRM---ASSIGKTADYALTLKNNTTKDKGDNIPKFEPPKLENLERKLQLLLGEWYEETYYKSDDVAALY-SFYQPTQGP-----FIIIPSMFKEEVTADFTLTIFSSQQVEVQKLEDSRNAVLSGKWSDKSAGGCHLYDKEYEQKADKFTWTNNPKFHMRFQTPEPTQVKITLSRPEKAWKKQIGMNLVGCMIGFYVYPG---NQQPTKDCLLNKLS--------------QKFVPWNEVSDE----LTLDGNPDGYIVMPTTYEPGKMGPFIISASTDVEFTL 928