prot_A-nodosum_M_contig1084.3.181 (polypeptide) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: D8LKP7_ECTSI (Wu:fa96e12 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKP7_ECTSI)

HSP 1 Score: 2538 bits (6577), Expect = 0.000e+0
Identity = 1535/2840 (54.05%), Postives = 1827/2840 (64.33%), Query Frame = 0

Query:    1 MAADVEGVERKLEELHRLSREGFSDVEINDPATLVENLGSAVLALDPHVPGNEADVALFASFVFDSQAPRDLLSFATSTMNTPAKVTKKARMAAIKVVGDFLKRIGGSLIIDRYALRVKQGLWDVFSKETESQEVRAHALRPLRQLLKLRPASLAAERWEVPAMWNAIKEQYRLG-SKVSQGVKGELLKLSGSLMLVHGADVPELAGEVTTVLGWCDSALTKHSENKRLQKAQQPQIVAGAFACIDLVCSAGFEERYAWSAAREGEGRNTTLYKHLVTAVAEGTVEDLTRFEVPAKALRFLRRHAGLFSNNVGPLPRALQLYGYMEKCAIFGGKSSRLKLKKHALPAIESVLRQVSNFAAASGDDSQRNTVYEFEKKFFALLEARRPTSEQTAVALAGLASLSAAATALSSYEEVTNVTDRLLACLDSALVRLDVVAFAEDDSEGEDDIAEPGAEGWGNDDGVALFSRDRSLKLRAQFLRALACRAGVLGGVFAKA---GRVAVSPEKRDDTCSAMDVEDDEGGEQEGGGRGKGKQGRAPDGATGAAVPGHVMRRLEEIATRLVQSYELLSRNDRGLACRALAQLWLALAGPGQSDNLSCLLASVVPKGVKRAGLMEEPGNGF---GETNSAAVHWVAPVNPITGVADKRLCFAQADLWRRVLDPGADDLEPLQAWSWRKGIQETAVG------------VVYREVVAPEVYDAVVGSVLTIIKRLDLRYE--TPEESVRPVALQPVDQQMFLNLVSFCQDLLPTCRADLLLEWAPIVCPELVERSLERPMVSGFYRLMTLMFRAIARAGFFDDRSPPPTASSSEKIDVTDKAWAGRASPGTVEDALSLETTGEGNRDLGASAPSGNLVLEADGGAGGSGRLVCRRVLC--RYVEWMAVRISGFQDELLSACTEALLSAPPDLLGGDLGPLVPAVKIALTSGASHLPTAVVAVAALERWNDENPTLLALYLGEILPHLDTHVLAEASVGGGDAGASEKARRRSKTGKTPKQSDAAVAVAEGRRGSEEATRKDLRNRIVRFLGRLGGMNQLVAADASLILSNSLAWDTKERIVLEVPYKNQKDKLPICLDAVLQRLVELARNEGTRQTKTLAAECLHALVVYMVGCTATDPSSGNRGEPGEYSKLFRRIFPAVLRLAVDLDETTRKLFSTLSMQLVRWFSQAQTENPDTIALLDCLGEGAASEEGEPLREMCAKGIVEFLRYAIKQSTKRQQARKQDLNYIYIYIFIYKGTCAEGPVTTDALLSRVFSLVMHPDKNRRLGGLMAFNQLCRPLREETSLVDRYALRLLHVALISLRRAHHDHSALGVVDAAAAAVNRSLRIVRHSVEELGDRADLLRHKDSRGQLGSVQEMAEWTWERVGSVETRFRRKCMAVLQALCPLIVGSLGPGL---PSDEAGTRVWVWRRLRTSGRDGVTEAVRIFEANNLGPRKSGRSAVCAGSHRGSNLVDPQDEDSADKDELDGAATSADCYKFACETGVITADELFLGDRENVPPFGSDGDDGEDDTRATGAQVAEGQGRTAGKKRSXXXXXXXXXEGSAENDSPGGDTRVSRGSPPRVLHMLATIAERFGSFLEGFPFQSLANEDANSSSTMAAESAASR--VASNVRGYGGCDRSSTRTLAPPESSSTGLCPRPLVVNDLGLLPTDRAAARLSRAIFLRRTFTLVAECLSST-AREEEMASLIVAEGLWSRGVHLLLAYSLLWPWAGGLLPRPSDGEEVEQFLPAASERLLKHWKILDRAAGRADKFSDTLRGLLRIRVSGDEGEKSSTSIGGGSIRGREVDLCQWLLSRSLSSQEALSRNTSVARSTMIDDVPRQPRESGGRRARAVFASSPEHLLTDEELVGRVLRGYRTFANAGLFDKTIASSISSSSGCGDPLYLSAGLWSSVLNLPEGLRPASEHRANTALRLAIELGLPITTTTNDGSVGIERDGLEKRGSKKVRRTGRETGGGYVVLPRAAEASIVAGVLDVCPAVRATTSGSSCETLPQEGDRQEVGGATRGALFYRRFSEPIHEALLRPSVDGRHTWREACASLVDACLSDGDAGFPGAGTVRAAQAFAVLEGVVTLQTDLRTPAATRCG--LMTPRETLAVVVPEFQRLVQHPYWAPPGAGSDSAGKNSPAV----TCRMLRESLRLCSRLLRLAAIEKSGR-VTTAAGRKTVFKMTPPSPGPYMRELLEMSGVLVAGTLGSSSPLVSPLAKADALALVPFLRPCLTRKAKEYGHDAPRSHSSR-----------SSADRSHSEVEMLPALEEMMSRHFPLEDSREPAQGSEEAKNFALLLRGLLKAFVRCGDLGLLELLFGTLAEGRKHLHYGWVSRALDAFVSGVDVDADGVAEAVFGFCLDKVSEQRVPINVKAALADKVCLPLLELCPVEISRSLYLSTTAGGSFSPGPKSASSSASVLQRLHMVVDRRNKETGKFLSGPLLVSICFALLEALYEMLELDALRALADQAFSKKDALKEVTKGVAKAWKAIKDGRTSDGAADQSWLQLRCRRAVFSCVCTVVRATQDQEKHFDALVWSDPLHDPTP-VGVDKESVLSLVLDMSKYHAFEVSPPRFQSRRLRPPRPPPLSANRGEGKGGRAR--LTASFMLSQSSLGVGGESFSGLGGFDQGASGGPLEDSRETYSSQAALTDSASQAVL-----TDKEHESSSQQDFDSHSQGPSLAPVFGRGAKGIGIFGGVPGGDNGREVEEETESGYDKDGDISLEMSHVNREPCMRSLLLVMQAEIRLFEKVWLDRFERSQRAYEPAWSSGMRAKLADPGLPRNVRLVAFQFALNTPAVEALKPWAKKWVVTMTDFALAPLPE 2785
            MAADVEGV+R LEELHR SRE F D EINDPA LVE L  AVLALDP  PG EADVALFASFVF+SQAPRDLLSF T T+N+ AKVTKK+RMAA+KVVG+F KR+ G+ +IDRYA R+K   W VFSKE ESQEVRAHALRPLR LLKLR  +LAAE+W V  MW  I+EQY LG SK+SQ  KGELLKL+GSL+LV+G DVPELA  V  +LGWCD+AL  ++++++L KA QPQIVAGA ACIDL+CSAGFEERY + A       NT LY HL+ AV EG+ +DL+RFEVPAKALRF RRHA LFS+N+GPL RA QLY  ME+CA  GG+SSRLKLKKHALPAIE+ LR+V+ + A+    + ++T+ + E  F  LLE  +P++E+  VALAGLA+LS AATALSSY++  +V DRLL CLD+A  +LD V FA +DS+ ED     G  G     G   + +D+SLKL AQFLR LACRAGVLGGVFA+    G  +       D    MDV D+EGG   G G+G G             VPGH+MRRL+ ++ RLV++Y LLS  DRGLAC+ L QLWLAL+GPGQ DNLS +L  VV KGV  AG ME PG G    GE + A V WVAPV+P+TG  D RLCF  ADLWRRV++PG+ +LEPLQAW    G    A              V+YR+ +APEVYDAV+G+VLT +KRLDLRY   TPEE  RP+ L   DQ MFLNLVSFCQDLLPTCR DLLLEW PI+CPELV+RSLERP+VSGFYRL+TL+FR  AR+G+FDD +  P     + +                                                            LC   Y+EWM VR+ GF+DELL+ACTE LLSAP  LLGGDL PLVPA++ ALTSG SHLPTAVVAV ALERW +E P LL  +LGEILPHLD H+ A     GGD G++EK RR SK GK            +     EE+ RKDLR RIVRFLGRLGG N LVA DAS  LS+SLAWDT  RI+L VP+K++++ L ICLD VLQRLVELA +EG RQTKTLAAECLHALVVYMVGCTATDPSSG +G PG++SKLFRR+FPAVLRLAVDLDETTR LFS LSMQLVRWFSQAQTEN DT+ALLDCLGEGAA+EEG PLRE+CAKG+VEFLRYAIKQSTKRQQA                    EGPV  DALL+RVFSL  HPDKNRRLGGLMAFNQLCRPLREET+L++RYALRLLHVALISLRRAHHDHSALGV DAA++AV+R+LR+V  S+ E GDR DLLR +D RG+L SV+EMA W W+RVG+VETRFRRKCM VL ALCPL+V +   GL   PS+  G R WVWRRL  SGR G+TEAVR+FE++    +    +AV  G  +        +    +  E D  AT+ADCY FA +TG+ITA ELFLG     P  G                              XXXXXXXXX            +  S  + P V+  LA + ERFG FLE FPFQSLA E+         +    R    +NV G          T+A   ++      RPLVV+D GLLPT+RAAA+LSRA+ LRR FTL+A CLS   AR +EMA+ +V EGLWSR  HL++ Y L+WPWAG  LPR SDG+EVEQFLPAA ERLLKHWK LD AAG  +  S+TL+ LLR+RVS ++  + S +    S     VDLCQW+L++SLSSQ+ALSR     R+          R+    R+ A  + +P     D+EL GRV+R YR+FAN+GLF            G G+   L++GLWS+V+ LPEGLRPASEHRA T LRL+++LGLP TT    G+                                   ASI+AG+LD+ PA RA  + +             VGGATRG+LFYRRFSEPIH+ALLR        W   C +LVDACLS       G   + AAQAF+VLEGV+ LQ++  T A T      M P ETL+V++PEF+RLV+ P W PP          S AV    T +MLRESLRLC RLLRLAA  KSG  + T+A   +    T P   PY   LLEMS +LVA TLGSSS +V    KADAL LVPFLRPC+TRKA+ YGH  P  H+ R           S+  +  S+  ++PA+EEMMS HFP+++SREPA+GSE+A  FALLLRGLL AFVRCGDLGLLELLFGTLAEGRKH HY WV +ALDAFVSG+D++A GVAEAV GFCLDK++  RV  +V+  L DK                      A  +F                       R K+T +   GP              E  E D  +AL          LK+VT GV  AWKA+     S+    Q+WL+LRCRRAVFSCVCT VRATQD+EK FD LVWSD L+  +   G  K SVL+LVL+M   H FEVSP  FQS RL P RPPPL   R     GR+R  +TAS MLSQSSLG GG+S + + G D GA+    ED RETYSSQAALT+ A          TD +H  S  Q     SQG  +APVFGRGA      GG  GG N    EE+          + LEMSHVN+EPCMRSLL VM A+ RLF   W ++ ++S   +EPAWS  MRAKL D  LPRNVR++AFQFALN P   AL PWA  W+  ++DFALAPLPE
Sbjct:    1 MAADVEGVQRLLEELHRHSRENFPDSEINDPAALVEQLSIAVLALDPRSPGQEADVALFASFVFESQAPRDLLSFVTDTINSSAKVTKKSRMAALKVVGNFSKRLKGTPVIDRYAQRIKDDTWAVFSKERESQEVRAHALRPLRALLKLRSPNLAAEKWNVAGMWATIREQYSLGTSKISQSCKGELLKLAGSLVLVYGRDVPELAEGVKIMLGWCDAALNDNAKSQKLGKALQPQIVAGALACIDLLCSAGFEERYTYKAGESASKPNTKLYMHLLRAVQEGSAQDLSRFEVPAKALRFFRRHARLFSDNIGPLSRARQLYDLMEQCATAGGRSSRLKLKKHALPAIEATLREVARYTASR--PANKDTLDKLEGTFLKLLEGAKPSAEEVTVALAGLAALSPAATALSSYQDAADVADRLLVCLDAARGQLDEVGFAGEDSDEED-----GGNGSPGARGTDSYGKDKSLKLEAQFLRTLACRAGVLGGVFARERQQGDASPGLTGGGDGVVGMDV-DEEGGPVAGKGKGGGVXXXXXX-XXXXXVPGHLMRRLQSLSLRLVENYALLSSKDRGLACQGLCQLWLALSGPGQGDNLSRMLGVVVQKGVSLAGTMENPGGGIDEAGEPSDAPVKWVAPVDPVTGAVDARLCFLYADLWRRVVNPGSAELEPLQAWEALPGSGAAAXXXXXXXXXXXXXXVLYRDAIAPEVYDAVLGAVLTAVKRLDLRYTYGTPEEGGRPIPLHATDQNMFLNLVSFCQDLLPTCRPDLLLEWVPILCPELVKRSLERPLVSGFYRLVTLVFRETARSGYFDDHTVSPETPCDQAV------------------------------------------------------------LCPPSYLEWMTVRLGGFRDELLAACTETLLSAPVGLLGGDLSPLVPALRAALTSGTSHLPTAVVAVEALERWREETPNLLWAHLGEILPHLDKHLSAGP---GGDGGSAEKVRR-SKGGKAAGSXXXXXXXXDT--AGEESRRKDLRRRIVRFLGRLGGRNHLVATDASKALSDSLAWDTTPRILLNVPFKDRQNPLTICLDGVLQRLVELALHEGARQTKTLAAECLHALVVYMVGCTATDPSSGQQGRPGDFSKLFRRVFPAVLRLAVDLDETTRTLFSKLSMQLVRWFSQAQTENDDTLALLDCLGEGAAAEEGGPLREVCAKGVVEFLRYAIKQSTKRQQA--------------------EGPVAVDALLTRVFSLATHPDKNRRLGGLMAFNQLCRPLREETALLNRYALRLLHVALISLRRAHHDHSALGVADAASSAVDRALRVVHESITEKGDRGDLLRKRDERGELRSVEEMAGWAWDRVGAVETRFRRKCMTVLLALCPLVVETFD-GLDETPSENDGARTWVWRRLEESGRAGITEAVRVFESSASQSQAFSSAAVGGGGFKAIGGEQGAELRENETMEWDRIATAADCYNFARDTGIITAAELFLG----APKRGX---------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSYGSEAAEPLVVRSLAGVVERFGKFLEDFPFQSLAGEEERDGDAGVVQGDGLRHPTVANVSG----------TVAARLAAEAS---RPLVVDDPGLLPTERAAAQLSRAVLLRRGFTLLAGCLSDDGARRDEMAAFLVKEGLWSRRAHLVVIYCLVWPWAGEFLPRASDGDEVEQFLPAAVERLLKHWKTLDNAAGPGNGLSETLQSLLRVRVSKEDSARLSAA-SPSSGEDDAVDLCQWILAQSLSSQQALSRPFVSGRAG---------RKPSTARSNARRSPTPP----DDELTGRVIRAYRSFANSGLFGSV-------GGGGGEAFRLASGLWSTVVRLPEGLRPASEHRAKTVLRLSMDLGLPTTTGATIGA-----------------------------------ASILAGLLDLHPAKRAAAASAV-----------GVGGATRGSLFYRRFSEPIHDALLRRGAGC--PWSSVCKALVDACLSTTAGETRGHHPLLAAQAFSVLEGVLILQSERATGATTLTSRATMDPSETLSVILPEFRRLVEDPDWGPPPTAVRGKTGGSAAVGVHGTGKMLRESLRLCHRLLRLAAAVKSGSPIATSASGSSGSSTTSP---PYFERLLEMSALLVARTLGSSSSVVDASTKADALTLVPFLRPCITRKARNYGHAVP--HAGRQGEGGXXXXXXSAGGKGKSDGLVMPAMEEMMSMHFPIDNSREPAEGSEQATAFALLLRGLLGAFVRCGDLGLLELLFGTLAEGRKHRHYRWVDKALDAFVSGLDMEASGVAEAVLGFCLDKLTAGRVGASVRTILFDKAL--------------------ADSAF-----------------------RKKKTSE--DGPA------------KERQEGDEEKAL---------TLKDVTLGVCNAWKAVV---ASEEKFSQNWLELRCRRAVFSCVCTAVRATQDKEKFFDKLVWSDALYAKSSGPGGGKTSVLALVLNMETEHTFEVSPKVFQSSRLLPARPPPLGRGRSAATTGRSRGGMTASSMLSQSSLGFGGDSLAVVAGGDGGAADPAAEDERETYSSQAALTEPAGVPEGDGEPGTDSQHPRSQSQ-----SQG-DMAPVFGRGAV-ESAGGGTRGGGN---AEEDA---------VFLEMSHVNKEPCMRSLLQVMVAQQRLFGDSWNEQVDQSNGMFEPAWSVKMRAKLEDIDLPRNVRILAFQFALNAPVSTALLPWAVPWIKALSDFALAPLPE 2556          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: A0A6H5L056_9PHAE (NUC194 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L056_9PHAE)

HSP 1 Score: 2433 bits (6306), Expect = 0.000e+0
Identity = 1486/2719 (54.65%), Postives = 1756/2719 (64.58%), Query Frame = 0

Query:  429 LVRLDVVAFAEDDSEGEDDIAE-PGAEGWGNDDGVALFSRDRSLKLRAQFLRALACRAGVLGGVFAKA---GRVAVSPEKRDDTCSAMDVEDDEGGEQEGGGRGKGKQGRAPDGATGAAVPGHVMRRLEEIATRLVQSYELLSRNDRGLACRALAQLWLALAGPGQSDNLSCLLASVVPKGVKRAGLMEEPGNGF---GETNSAAVHWVAPVNPITGVADKRLCFAQADLWRRVLDPGADDLEPLQAWSWRKGIQETA--------VGVVYREVVAPEVYDAVVGSVLTIIKRLDLRYE--TPEESVRPVALQPVDQQMFLNLVSFCQDLLPTCRADLLLEWAPIVCPELVERSLERPMVSGFYRLMTLMFRAIARAGFFDDRSPPPTASSSEKIDVTDKAWAGRASPGTVEDALSLETTGEGNRDLGASAPSGNLVL---------------------------EADGGAGGSGRLVCRRVLCR---YVEWMAVRISGFQDELLSACTEALLSAPPDLLGGDLGPLVPAVKIALTSGASHLPTAVVAVAALERWNDENPTLLALYLGEILPHLDTHVLAEASVGGGDAGASEKARRRSKTGKTPKQSDAAVAVAEGRRGSEEATRKDLRNRIVRFLGRLGGMNQLVAADASLILSNSLAWDTKERIVLEVPYKNQKDKLPICLDAVLQRLVELARNEGTRQTKTLAAECLHALVVYMVGCTATDPSSGNRGEPGEYSKLFRRIFPAVLRLAVDLDETTRKLFSTLSMQLVRWFSQAQTENPDTIALLDCLGEGAASEEGEPLREMCAKGIVEFLRYAIKQSTKRQQARKQDLNYIYIYIFIYKGTCAEGPVTTDALLSRVFSLVMHPDKNRRLGGLMAFNQLCRPLREETSLVDRYALRLLHVALISLRRAHHDHSALGVVDAAAAAVNRSLRIVRHSVEELGDRADLLRHKDSRGQLGSVQEMAEWTWERVGSVETRFRRKCMAVLQALCPLIVGSLGPGL---PSDEAGTRVWVWRRLRTSGRDGVTEAVRIFEANNLGPRKSGRSAVCAGSHRGSNLVDPQDEDSADKDELDGAATSADCYKFACETGVITADELFLGDRENVPPFGSDGDDGEDDTRATGAQVAEGQGRTAGKKRSXXXXXXXXXEGSAENDSPGGDTRVSRGSP---PRVLHMLATIAERFGSFLEGFPFQSLANEDANSSSTMAAESAASR--VASNVRGYGGCDRSSTRTLAPPESSSTGLCPRPLVVNDLGLLPTDRAAARLSRAIFLRRTFTLVAECLSST-AREEEMASLIVAEGLWSRGVHLLLAYSLLWPWAGGLLPRPSDGEEVEQFLPAASERLLKHWKILDRAAGRADKFSDTLRGLLRIRVSGDEGEKSSTSIGGGSIRGREVDLCQWLLSRSLSSQEALSRNTSVARSTMIDDVPRQPRESGGRRARAVFASSPEHLLTDEELVGRVLRGYRTFANAGLFDKTIASSISSSSGCGDPLYLSAGLWSSVLNLPEGLRPASEHRANTALRLAIELGLPITTTTNDGSVGIERDGLEKRGSKKVRRTGRETGGGYVVLPRAAEASIVAGVLDVCPAVRATTSGSSCETLPQEGDRQEVGGATRGALFYRRFSEPIHEALLRPSVDGRHTWREACASLVDACLSDGDAGFPGAGTVRAAQAFAVLEGVVTLQTDLRTPAATRCG--LMTPRETLAVVVPEFQRLVQHPYWAPPGAGSDSAGKNSPAV----TCRMLRESLRLCSRLLRLAAIEKSGR-VTTAAGRKTVFKMTPPSPGPYMRELLEMSGVLVAGTLGSSSPLVSPLAKADALALVPFLRPCLTRKAKEYGHDAPRSH---------SSRSSADRSHSEVEMLPALEEMMSRHFPLEDSREPAQGSEEAKNFALLLRGLLKAFVRCGDLGLLELLFGTLAEGRKHLHYGWVSRALDAFVSGVDVDADGVAEAVFGFCLDKVSEQRVPINVKAALADKVCLPLLELCPVEISRSLYLSTTAGGSFSPGPKSASSSASVLQRLHMVVDRRNKETGKFLSGPLLVSICFALLEALYEMLELDALRALADQAF-------------------SKKDALKEVTKGVAKAWKAIKDGRTSDGAADQSWLQLRCRRAVFSCVCTVVRATQDQEKHFDALVWSDPLHDPTP-VGVDKESVLSLVLDMSKYHAFEVSPPRFQSRRLRPPRPPPLSANRGEGKGGRAR--LTASFMLSQSSLGVGGESFSGLGGFDQGASGGPLEDSRETYSSQAALTDSASQAVLTDKEHESSSQQDFDSHSQGPS-LAPVFGRGAKGIGIFGGVPGGDNGREVEEETESGYDKDGDISLEMSHVNREPCMRSLLLVMQAEIRLFEKVWLDRFERSQRAYEPAWSSGMRAKLADPGLPRNVRLVAFQFALNTPAVEALKPWAKKWVVTMTDFALAPLPEGLRVDGEPGLHYLLRDFAHVLLDETN-KGWGGAVREARAEERRCASTGDDGGDGPLRRHQPLLDAGNRLVAHLMSVSSYPPGKTPMLRANLELISGLIGLFGDPGRGSTGGDGNSHVSAGGDDDFAAAAPIRNELLLNVRCVVDMLGEREGHTGGAHARAQSRGAQAKHTILAGLNLLGVLFARNVPVFASSVPAADELARTIPKVCLSYTQKNAFETGTELLAIALAHVSWLKDGGGLPPGNILCEQCRSDEG-EWLARAVTEQLI 3050
            L +LD V FA +DS+ ED   + PGA G  +      + +DRSLKL AQFLR LACRAGVLGGVFA+A   G  +       D    MDV D+EGG   G G+G G             VPGH+MRRL+ ++ RLV++Y LL   DRGLAC+ L QLWLA +GPGQ DNLS +      KGV  AG ME PG G    GE +   V WVAPV+P+TG  D RLCF  ADLWRRV++PG+ +LEPLQAW    G    A         GV+YR+ +APEVYDAV+G+VLT +KRLDLRY   TPEE  RP+ L   DQ MFLNLVSFCQDLLPTCR DLLLEW PI+C ELV+RSLERP+VSGFYRL+TL+FR  AR+G+FDD +P             D+A     SPG+V  A+ L+    G   LG                                     E  G AG   R+ CRR+LCR   Y+EWM VR+ GF+DELL+ACTE LLSAP  LLGGDL PLVPA++ ALTSG SHLPTAVVAV ALERW +E P LL  +LGEILPHLD H+ A     GGD G++EK RR SK GK             G    E + RK+LR RIVRFLGRLGG N LVA DAS  LS+SLAWDT  RI+L+VP+K++++ L ICLD VLQRLVELA +EGTRQTKTLAAECLHALVVYMVGCTATDPSSG +G PG++SKLFRR+FPAVLRLAVDLDETTR LFS LSMQLVRWFSQAQTEN DT+ALLDCLGEGAA+E+G PLRE+CAKG+VEFLRYAIKQSTKRQQA                    EGP   DALL+RVFSL  HPDKNRRLGGLMAFNQLCRPLREET+L++RYALRLLHVALISLRRAHHDHSALGV DAA++AV+R+LR+V  SV E GDR DLLR +D RG+L SV+EMA W W+RVG+VETRFRRKCMAVL ALCPL+V +   GL   PS+  GTR WVWRRL  SGR G+TEAVR+FE++    +    +AV  G  +        +    +  E D  AT+ADCY FA +TG+ITA ELFLG  +                R                   XXXXXXXXX               S GS    PRV+  LA + ERFG FLE FPFQSLA  +         +   +R    +NV G            A   + ++G    PLVV+D GLLPT+RA A+LSRA+ LRR FTL+A CLS   AR +EMA+ +V EGLWSR  HL++ YSL+WPWAG LLPR SDG+EVEQFLPAA ERLLKHWK LD AAG  +  S+TL+ LLR+RVS ++  + S +    S     VDLCQW+L++SLSSQ+ALSR    +R+          R+    R+ A  + +P     D+EL GRV+R YR+FANAGLF      S+    G G    L++GLWS+VL LPEGLRPASEHRA TALRL+++LGLP TT    G                                    ASI+AG+LD+ PA RA  S +             VGGATRG+LFYRRFSEPIH+ALLR        W   C +LVDACLS       G   + AAQAF+VLEGV+ LQ++  T A T      M PRETL+VVVPEF+RLV++P W PP          S AV    T +MLRESLRLC  LLRLAA  KSG  + T+    +    T P   P+   LLEMS +LVAGTLGSSS +V    KADAL LVPFLRPCLTRKA+ YGH  P +          +  S+  +   +  ++PALEEMMS HFP+++SREP +GSE+A  FALLLRGLL AFVRCGDLGLLELLFGTLAEGRKH HY WV +ALDAFVSG+DV+A GVAEAV GFCLDK++  RV  +V+  L DKVCLP+L  CP EI +SLYLS TAG  F P  K       VL+RL  VV +R+K  G FL GPL+ S CF+LLE LY+  +L+ L+ALAD AF                    K   LK+VT GV  AWKA+     S+    QSWL+LRCRRAVFSCVCT VRATQD+EK FD LVWSD L+  +   G  K SVL+LVL+M   H FEVSP  FQS RL P RP PL   R     GR+R  +TAS MLSQSSLG GG+S + + G D GA+    ED RETYSSQAALT+ A   V        +  Q   SHSQ    +APVFGRGA      GG  GG            G  ++  I LEMSHVN+EPCMRSLL VM A+ RLFE  W ++ ++S   +EPAWS  MRAKL D  LPRNVR++AFQFALN P   AL PWA  W+  ++DFALAPLPEGLR DGE GLHYLLRDFA +LLDE+   GWG AVREARAEERR A+  + GGD      QPLLD GNRLV+HLMSV+S P GKTPMLRAN+ L++GL+GLFGD     T  +G++    G            N+L LN++ VVDML EREGHTGGAHA  QS+G QAKHTIL GLNLLGVL    VPV  SSVPAAD+LART+P VCLSYTQKN FETGTELLA+ALAHVSWL++ GGLP G+ LC   R  EG EWLAR VT++L+
Sbjct:   23 LAQLDEVGFAGEDSDEEDGGNDSPGARGTDS------YGKDRSLKLEAQFLRTLACRAGVLGGVFARARQQGDASPGSTGGSDGVVGMDV-DEEGGHVAGKGKG-GXXXXXXXXXXXXXVPGHLMRRLQSLSLRLVENYALLGNKDRGLACQGLCQLWLAFSGPGQGDNLSRM------KGVSLAGTMENPGGGVDEAGEPSDTPVKWVAPVDPVTGAVDARLCFLYADLWRRVVNPGSAELEPLQAWEAVPGSGAAAGAAAVSGGSGVLYRDAIAPEVYDAVLGAVLTAVKRLDLRYTYGTPEEGGRPIPLHATDQNMFLNLVSFCQDLLPTCRPDLLLEWVPILCSELVKRSLERPLVSGFYRLVTLVFRETARSGYFDDNTPETPC---------DQAVLSPPSPGSVVTAIGLDM---GGGSLGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAARERVGDAGE--RINCRRILCRRVSYLEWMTVRLGGFRDELLAACTETLLSAPVGLLGGDLTPLVPALRAALTSGTSHLPTAVVAVEALERWREETPNLLWPHLGEILPHLDKHLSAGR---GGDGGSAEKVRR-SKGGKAA--GXXXXXXXXGDTAGEGSRRKELRRRIVRFLGRLGGRNHLVATDASQALSDSLAWDTTPRILLKVPFKDRQNPLTICLDGVLQRLVELALHEGTRQTKTLAAECLHALVVYMVGCTATDPSSGQQGRPGDFSKLFRRVFPAVLRLAVDLDETTRTLFSKLSMQLVRWFSQAQTENDDTLALLDCLGEGAAAEDGGPLREVCAKGVVEFLRYAIKQSTKRQQA--------------------EGPAAVDALLTRVFSLATHPDKNRRLGGLMAFNQLCRPLREETALLNRYALRLLHVALISLRRAHHDHSALGVADAASSAVDRALRVVHESVTEKGDRGDLLRKRDERGELRSVEEMAGWAWDRVGAVETRFRRKCMAVLLALCPLVVETFD-GLDETPSENDGTRTWVWRRLEESGRAGITEAVRVFESSASQSQAFSSAAVGGGGFKAIGCEQGAELRENETMEWDRIATAADCYNFARDTGIITAAELFLGAAK----------------RGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSYGSEAAEPRVIRSLAGVVERFGKFLEDFPFQSLAGAEERDGDACVVQRGGARHPTVANVSGT---------VAARLAAEASG----PLVVDDPGLLPTERATAQLSRAVLLRRGFTLLAGCLSDDGARRDEMAAFLVKEGLWSRRAHLVVIYSLVWPWAGELLPRASDGDEVEQFLPAAVERLLKHWKTLDNAAGPGNGLSETLQSLLRVRVSKEDSARLSAA-SPSSGEDDAVDLCQWILAQSLSSQQALSRPFVSSRAG---------RKPSTARSNARRSPTPP----DDELTGRVIRAYRSFANAGLFG-----SVGDGGGGGKAFRLASGLWSTVLRLPEGLRPASEHRAKTALRLSMDLGLPTTTGATTGV-----------------------------------ASILAGLLDLHPAKRAVASSAV-----------GVGGATRGSLFYRRFSEPIHDALLRRGAGC--PWSSVCKALVDACLSTTAGETRGHHPLLAAQAFSVLEGVLILQSERATGATTLTSRATMDPRETLSVVVPEFRRLVENPDWGPPPTAVRGKTGGSAAVGVHGTGKMLRESLRLCHCLLRLAAAVKSGSPIATSTSGSSGSSTTSP---PHFERLLEMSALLVAGTLGSSSSVVDASTKADALTLVPFLRPCLTRKARNYGHAVPHAGKQGEDGAGGAGASAGGKGKPDGLVMPALEEMMSMHFPIDNSREPEEGSEQATAFALLLRGLLGAFVRCGDLGLLELLFGTLAEGRKHRHYRWVDKALDAFVSGLDVEASGVAEAVLGFCLDKLTAGRVGASVRTVLFDKVCLPMLRRCPAEIVKSLYLSATAGSWFRPVSKGKVVEGPVLRRLFAVVGKRSK-AGTFLDGPLVASCCFSLLEVLYDAFDLEPLQALADSAFRNTKTIEGGPAKEPQEGDEEKALTLKDVTLGVCNAWKAVV---ASEEKLSQSWLELRCRRAVFSCVCTAVRATQDKEKFFDKLVWSDALYAKSSGPGGGKTSVLALVLNMETEHTFEVSPKVFQSSRLFPARPLPLGRGRSAATTGRSRGGMTASSMLSQSSLGFGGDSLAVVAGGD-GAADPAAEDERETYSSQAALTEPA--GVPEGDGEPGTDSQHPRSHSQSQGDMAPVFGRGAV-ESAGGGTRGG------------GVAEEDVIYLEMSHVNKEPCMRSLLQVMVAQRRLFEDSWNEQVDQSNGMFEPAWSVKMRAKLEDIDLPRNVRILAFQFALNAPVSTALLPWAVPWIKALSDFALAPLPEGLRTDGENGLHYLLRDFAQLLLDESGASGWGRAVREARAEERRRAA--EAGGDPGDVSRQPLLDVGNRLVSHLMSVASCPDGKTPMLRANVLLVAGLVGLFGDKVVEQTDHNGDAIRMEG------HRTGRTNDLRLNLQFVVDMLKEREGHTGGAHASTQSKGVQAKHTILTGLNLLGVLLGMKVPVLTSSVPAADQLARTLPDVCLSYTQKNTFETGTELLAMALAHVSWLEEDGGLPSGDSLCPVLRQAEGSEWLARRVTDRLM 2559          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: D8LKP6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKP6_ECTSI)

HSP 1 Score: 939 bits (2427), Expect = 4.650e-293
Identity = 656/1447 (45.34%), Postives = 826/1447 (57.08%), Query Frame = 0

Query: 3463 MGPPAPRLPGVR---PRGRGVGVNSQAGIGGVGKRFRQSAEGSVSSLGHGESHAMQLRVAAKRERDKAARAKRAVIYRKYRDGELPDIQIKLGDILRPLQHLSMLDGGIAREVFVQVFKAVYAKLDSGSGSGSPPHGALKRGGVDGEDDEW--EASEGTGMRAVSLLLQRMLSA-------------------------------------------------------------------TKT-----------ETGVVACLLAACRAAAAAGGCDIEAPLYENVVGGRETSGRKGRGGGSSQP----------RRGRLMTPSSGTAATINQSGKK----TGAESQPRASEPDGGAIVLNPKLVADSAIASFNFHGGILLLEEAIIRSGRE----SVXXXXXXXXXXXXXXXXXXXXSSGGRAGTIDMHPGEDGLGASV----EGQQDHWLQLSRLYAALGERDVVVGVSARASRLEGTRQALDCELSGDFNGALKMYRELVRRYDSA--EMDDDEGADAAK----DAMIAASETELLDWDMHQLECLLQLGDWEGVREQVLAILPEDEEDESR------GGDADAATECRRKLWKADPSDRDGTLPYLVRSLLDDESRREEHLRDFLEDVLDPGAPLLRLAESYAPAGASGVS--------------------------------SSCSRLEWLEANMPGELARAFLLEGELGRAEQNIQRCYERFQETWAALHPCADSARREQLRGLQVAVEVEDFLLFADQRATTGHRTLTRTAPSPRSIPSSAWRSGTTAAVSPFCDRLRGLLERWWRQVPSRTLDPVAWWRVVRTHRHDCFTAIEGFLEDEGGLSNQARLDCKRDMMKHRADLSLVAAGAAVAQGVDSVAKELITSYTTVKKQAERLWSKGKRSHMKTELEPGEAGAGGAEDWDLELTLTVVEFQRTKLERALLAGKRNGADALKKREAMISGVLRIINGTTRQHHAAMLEAEARRAXXXXXXXXXXXXXXGEWGAMWGWKSEGAAAYETRLRLLTLQGQWLEMEGRVMAEGTTAAGGERESTPSSDLAGASNSLTAALASLQEAANLGDRLISAPVGVDAAVGVSTASPSLAECVKTTGIVEATGEATLRLAKLCEKLAVGDGIVEAGVGGGXXXXEELAALAAEHYLRAMAMGSKDARDHFPRVLFLVGEFPEACDAFSARASSVPEWTFLRWISQMLGVTGRREGPAVIKVLEGVAKVYPRALFYPLLMTKASSSSRRTEG----DDDDAKLCRLTAMVFDPSAEAFAEALRGLHDPQLRFCDDMKRVVELLRTGQKDLALKTYLKMRSECLVTVGLKT-NSKIGQLNREFAKQYGPEVDQALGKDGQRLDLAKAKKCLDKGLIWAGRARAKKTGGTARLSHLSSWMSDFDSNVHTLEVPGQYGGISHGPPRVSHHSRIISFGSELLV 4755
            M PPAPR PG R   PRG G GV+SQAG  GVG+RF +++ GSVSS+  GE++AMQ R+AAKR RDK  RAKRAVIYRKYRDGELPDI IKLGD+LRPLQHLSMLDGGIAREVFVQ+FK VYA     + + S    A K       DDE   EAS G G RAVS+LLQRMLSA                                                                   T+T           +T VVACL AACRA A A    +  P Y +   G                           +GR++ PS         +G++    T  E     +   G AI L+P LVA++A++S N H GILLLEE+I+R G+E    + XXXXXXXXXXXXXXXXXXXX               D     V    +GQQD WLQLS+LYA+LGERD +VGV+ARAS+ EGTRQAL+ ELSG+++ AL+MY++L+ RYD+   +  D + + AA+    + +  ASE EL  WDMHQLEC+ QLG W+ +R +V+A++ E  E E        G DA  + EC+R+LW ADPS RD  LP+ V S++ D  R E  L  FL+ ++DP AP LRL +S    G  G +                                S+C+R+ WLE NMP ELA+AFLL  +LGRA Q + RCY+RF E WA LHPCADSARR+QLRGLQ+ VEVEDFL F D++ TT        A S +            A       RL  LL++W RQ PS+ LDPV  W  +  HR DCF AIE  LE E  L  + R+  +RDM+KH  DLS+VAAGAA AQGV SVA +LI SY  +K  A RL   G  S  + +  P   G  G ++WDL+LTLT VE  R +LER  LAG R GAD  +K+   ++  L+++ G   Q   A+     +RA                WG   GW+++GA A+ETRLRLLTLQGQWLE +G ++AE                   A+ S  A+LASL++A  LGD L   P G   +    +++ + +    T G VEATGEA LRLAKLC+ ++ GD  +  GVGG     E+LAALA + YLRAMA+GS  AR  FPRVL+L G+F +A  AF+  AS+VPEW FLRWISQMLGVTGR EGP VI+                     AS+++R  EG    DDD  +L RLTA+  DPSAEAFAEALRGLH P++RF D MKRV  LL   QKDLA   Y K+R++CLVT G KT + K+G  NREFAK++  E+D+A+GKDG+ L+  +A++ L +G  W  +   KK+    +LS  S+WMSDFDSN H LEVPGQYGG+S  PPRVS HSRI+     L V
Sbjct:    1 MPPPAPRAPGTRGSGPRGGGGGVSSQAG--GVGRRFWRTSGGSVSSIAKGETYAMQKRMAAKRARDKTTRAKRAVIYRKYRDGELPDIMIKLGDVLRPLQHLSMLDGGIAREVFVQIFKEVYAGFGGSARNPSASQPADKARTQPELDDEIQREASVGEGRRAVSVLLQRMLSAARYAVAGGGCSVIVVVGGPSFVPALPCFFCLSPYFLAAFSLSVLSRLSLFPPLPSGYSRQQTTLTNPTETKYALASFHKRHDTDVVACLHAACRATATA----VVNPPYTDSGDGXXXXXXXXXXXXXXXXXXXXXXXXXHHKGRII-PSXXXXXXXXXTGRRARIATAEEPIEEVTVEGGDAISLSPDLVANTALSSLNVHSGILLLEESIMRIGKERAAVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDEKNVHVVDVEKGQQDAWLQLSKLYASLGERDALVGVAARASKSEGTRQALEAELSGEYDVALRMYKKLLDRYDTRFEKETDTQDSSAARGMDEEGVEGASEAELRAWDMHQLECMRQLGQWKTLRGEVVAMVSEGVEGEDEIGGEEYGDDARGSAECQRRLWGADPSQRDDVLPFFVWSIVHDHRRSEIALNAFLDAIVDPEAPSLRLTDSSWGGGEGGTAGESSSLALRSGAEXXXXXXXXXXXXXXXXPSASTCTRMAWLETNMPAELAKAFLLGNQLGRAHQCVARCYDRFHEKWAGLHPCADSARRDQLRGLQLVVEVEDFLSFYDKKGTTTSXXXXXLASSSKG-----------AVAVGLGGRLNRLLDKWSRQTPSQALDPVTSWTAMLAHRQDCFKAIEIDLE-EAELPEKQRVALRRDMLKHTGDLSIVAAGAATAQGVHSVAGKLIKSYADIKDSAGRL---GTASDKQGQAPPTR-GTDGDDNWDLQLTLTAVELNRVRLERLRLAGNRTGADLARKQADYVARTLKVLGGCISQQQQAI----GKRASGGSRG----------WGEGEGWETQGAEAHETRLRLLTLQGQWLETKGLLLAENXXXXXXXXXX--------AAESFCASLASLEKATELGDGLTVRPTGSKQSPTPGSSTGNASGTTPTPGAVEATGEAMLRLAKLCDAMSAGDSTMS-GVGG--RGREDLAALAVKEYLRAMAVGSAGARHSFPRVLYLAGKFSKARAAFADGASAVPEWAFLRWISQMLGVTGREEGPVVIQ---------------------ASAAARNGEGGAGADDDGRRLSRLTALTADPSAEAFAEALRGLHHPEMRFSDGMKRVCGLLNKSQKDLARVAYRKLRNDCLVTTGRKTGDDKVGSYNREFAKKHIRELDKAMGKDGELLNEREARRFLFQGRKW--QLEVKKSS-RLKLSGFSTWMSDFDSNRHKLEVPGQYGGMSAAPPRVSLHSRIVRHERALFV 1375          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: D8LKP5_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKP5_ECTSI)

HSP 1 Score: 563 bits (1452), Expect = 7.080e-178
Identity = 296/417 (70.98%), Postives = 335/417 (80.34%), Query Frame = 0

Query: 4756 MKSLRMPKRLTMHANDGKDHMFLVKGGEDLRVDERVEQLFEVMNAVMAASPGCRRARLTNKTYQVVPVTPDIGMIEWVQDTRPLKSVIEEGLGQRLDGHEAHALFIKWLRG---DVGAAKFHAMFKTASRAEVEGAFAKRCASVLPTDALRRHLIAMAPSPEAFLTCRGGFARSLATLSACSYMLGIGDRHLDNFLLDTTTGTVVGIDFGAAFGVGTTQLGVPELIPFRLTHQFQTLLQPLDSVGLLRGYMISTLQALRGSQEVLLATLDVFLNEPVIDWLSHGAGDRQ----KGRGKGTYEXXXXXXXHRPGTGSSQPRPRSETGRKIQNSRRKLQGENPVPVLVDDLNQNLIVKKFGSLKKLEEIAWGHSGKRFRAKPELREGLLSVEDQVDCLIDLATDPDVLGRQWIGLTTWV 5165
            M SLR+PKRLTMH +D KDHMFLVKGGEDLRVD+RVEQLFEVMNAVMA S GCRRARL+N TY+VVPVTP+IG+IEWVQ+TRPLKSVIE+GLG +L    A   F K+L+       A  FH MFKT  R +VE  FA  CA+V PTDALR+HL+AMAPSPEAFLTCRGGFARSLATL+ACSY+LGIGDRHLDNFLLDT TGTVVGIDFGAAFG+  T+LGVPELIPFRLT+QFQ LLQPLDSVGLL+G+M+STLQALR  +EVLLAT+DVFLNEPV+DW+S G   RQ    +G G G+ E             + QPRP  E  RKI+N++RKL GENP  VL+DDL QNL VK+ GSL KLE I+WG SG R R+KPE REG LSVEDQVDCLIDLATDPDVLGRQW+GL TWV
Sbjct:    1 MNSLRLPKRLTMHGDDEKDHMFLVKGGEDLRVDQRVEQLFEVMNAVMATSAGCRRARLSNTTYKVVPVTPEIGLIEWVQNTRPLKSVIEQGLGTKLGSLSALDTFGKFLKAKPKQFPAPNFHEMFKTEGRPKVEEMFATCCATV-PTDALRKHLLAMAPSPEAFLTCRGGFARSLATLNACSYVLGIGDRHLDNFLLDTMTGTVVGIDFGAAFGIAHTELGVPELIPFRLTNQFQNLLQPLDSVGLLKGHMVSTLQALRAGREVLLATMDVFLNEPVMDWISEGVERRQAKTKRGGGGGSGEDAADEGL------TGQPRPPFEARRKIKNAKRKLLGENPAAVLIDDLGQNLSVKQLGSLAKLEAISWGSSGDRVRSKPEHREGWLSVEDQVDCLIDLATDPDVLGRQWVGLATWV 410          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: A0A6H5KSS2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSS2_9PHAE)

HSP 1 Score: 418 bits (1074), Expect = 1.430e-123
Identity = 307/666 (46.10%), Postives = 382/666 (57.36%), Query Frame = 0

Query: 3549 IKLGDILRPLQHLSMLDGGIAREVFVQVFKAVYAKLDSGSGSG--------SPPHG-ALKRGGVDGEDDEWEASEGTGMRAVSLLLQRMLSATKTETGVVACLLAACRAAAAAGGCDIEAPLYENVVGGRETSGRKGRGGGSSQPRRGRLMTPSSGTA-------ATINQSGKKTGAESQPRASEPDGGAIVLNPKLVADSAIASFNFHGGILLLEEAIIRSGRESVXXXXXXXXXXXXXXXXXXXXSSGGRAGTIDMHPGEDGLGASVEGQQDHWLQLSRLYAALGERDVVVGVSARASRLEGTRQALDCELSGDFNGALKMYRELVRRYDSA--EMDDDEGADAAK----DAMIAASETELLDWDMHQLECLLQLGDWEGVREQVLAILPEDEEDESR------GGDADAATECRRKLWKADPSDRDGTLPYLVRSLLDDESRREEHLRDFLEDVLDPGAPLLRLAESY---APAGASGVSS-----------------------------SCSRLEWLEANMPGELARAFLLEGELGRAEQNIQRCYERFQETWAALHPCADSARREQLRGLQVAVEVEDFLLFADQRATTGHRTLTRTAPSPRSIPSSAWRSGTTAAVSPFCDRLRGLLERWWRQVPSRTLDPVAWWRVVRTHRHDCFTAIE 4154
            IKLGD+LRPLQHLSMLDGGIAREVFVQ+FKA+  K++  +G G        S P G A  +  +DGE    EAS G G RAVS+LLQRML A + +T VVACL AACRA A A    +  P  +   G                                     AT  ++   T  ES   A+   G AI L+P LVA +A++S N H GILLLEE+I+R G E  XXXXXXXXXXXXXXXXXXXX                        ++D WLQLS+LYAALGERD +VGV+ARAS+ EGTRQAL+ ELSG+++ AL+MY++L+ RYD+   +  D + + AA+    + +  ASE EL  WDMHQLEC+ QLG W+ +R +V+A++ E  E E        G DA  + EC+R+LW ADPS RD  LP+ V S++ D  R E  L  FL+ ++DP AP LRL +S       GA+G SS                             +C+R+ WLE NMP ELA+AFLL  +LGRA Q + RCY+RFQE WA LHPCADSARR+QLRGLQ+ VEVEDFL F D+  TT       T       PS++   G  A       RL  LL +W RQ PS+ LDPV  W  V  HR DCF AIE
Sbjct:    2 IKLGDVLRPLQHLSMLDGGIAREVFVQIFKAL-CKVEVYAGFGGSARDPPVSQPAGKARTQPELDGEIQR-EASSGEGRRAVSVLLQRMLPAARHDTDVVACLHAACRATATA---VVNPPSTDRGDGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDATGRRARTATAQESSEDAAVEGGDAISLSPDLVASTALSSLNVHSGILLLEESIMRIGNERAXXXXXXXXXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXKKDAWLQLSKLYAALGERDALVGVAARASKSEGTRQALEAELSGEYDVALRMYKKLLDRYDTRFEKETDTQDSSAARGMDEEGVEGASEAELRAWDMHQLECMRQLGQWKTLRGEVVAMVSEGVEGEDDIGGEEYGDDARGSAECQRRLWGADPSQRDDVLPFFVWSIVHDHRRSEVALNAFLDAIVDPEAPSLRLTDSSWGGREGGAAGESSPXALRXXXXXXXXXXXXXXXXXXXXXSASTCTRMAWLETNMPAELAKAFLLGNQLGRAHQCVARCYDRFQEKWAGLHPCADSARRDQLRGLQLVVEVEDFLSFYDKEETT-------TXXXXXXFPSNS--KGVVAVGLGLGGRLNRLLSKWSRQTPSQALDPVTSWTAVLAHRQDCFKAIE 651          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: A0A4D9D184_9STRA (Non-specific serine/threonine protein kinase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D184_9STRA)

HSP 1 Score: 453 bits (1165), Expect = 3.000e-123
Identity = 708/2797 (25.31%), Postives = 1061/2797 (37.93%), Query Frame = 0

Query: 2720 ERSQRAYEPAWSSGMRAKLAD---------------------PGLPRNVRLVAFQFALNTPAVEALKPWAK-----KWVVTMTDFALAPLPEGLRVDGEPGL-HYLLRDFAHVLLDETNKGWGGAVREARAEER---------RC--------ASTGDDGGDGPLRRHQPLLDAGNRLVAHLMSVSSYPPGKTPMLRANLELISGLIGLFGDPGRGSTGGDGNSHVSA---GGDDDFAAAAPIRNELLLNVRCVVDMLG---------EREGHTGGAHARAQS-RGAQAKHTILAGLNLLGVLF-ARNVPVFASSVPAADELARTIPKVCLSYTQKNA---FETGTELLAIALAHVSWLK--------DGGGLPPGNILCEQ-----------CRSDEGEWLARAVTEQLISKGRSGTKRGD-----FLHAVATIAGSYPRVL-------SRRL---LVTSIEAMQLADASRRGRFLAMLADAFPRGHVPALEVQDLFTRLQPFLPSLLLETEEEFIRGSKQAGAPSDWVPVAQLSALRLVRALVPTMTE----------------KQ--------LGAILGE--DDAHSLVGLATPS--TYPACREVVYDTLLYLYT------------------SSQQRFSKETDDGNGARSGGAPLNREKAEAVRCALLRGLSDPDDEGMTFDDSGADASDRGELDDS----ARRGIRRRLLDVFEGGLSDEV-------PGDRLLYIMRNMFDARTARSWLRFAPYLMLRPAEKAPDYDGLMFKKRLAACEFVDMPVA-----GGIGTLDARP----MEPTFSF---ASLQSQXXXXXDEG-ARNYGDGGSYGTFSTDVDVRQAGMVRSTQAY---LWSQTQGPGGAERPRN-----------------------IAFLSQTQSRML----AAGP---------VDSSAARSVMGPPAPRLPGVRPRGRGVGVNSQAGIGGVGKRFRQSAEGSVSSLGHGESHAMQLRVAAKRERDKAARAKRAVIYRKYRDGELPDIQIKLGDILRPLQHLSMLDGGIAREVFVQVFKAVYAKLDSGSGSGSPPHGALKRGGVDGEDDEWEASEGTGMRAVSLLLQRMLSATKTETGVVACLLAACRAAAAAGGCDIEAPLYENVVGGRETSGRKGRGGGSSQPRRGRLMTPSSGTAATINQSGKKTGAES--QPRASEPDGGAIVLNPKLVADSAIASFNFHGGILLLEEAIIR-SGRESVXXXXXXXXXXXXXXXXXXXXSSGGRAGTIDMHPGEDGLGASVEGQQDH---WLQLSRLYAALGERDVVVGVSARA----------------SRLEGTRQALDCELSGDFNGALKMYRELVRRYDSAEMDDDEGADAAKDAMIAASETELLDWDMHQLECLLQLGDWEGVREQVLAILPEDEEDESRGG---DADAATECRRKLWKADP---SDRDG-------TLPYLVRSLLDDES------RREEHLRDFLEDVLDPGAPLLRLAESYAPAGASGVSSSCSRLEWLEANMPGELARAFLLEGELGRAEQNIQRCYERFQETWAALHPCADSARREQLRGLQVAVEVEDFLLFADQRATTGHRTLTRTAPSPRSIPSSAWRSGTTAAVSPFCDRLRGLLERWWRQVPSRTLD---PVAWWRVVRTHRHDCFTAIE--GFLEDEGGLSNQARLDCKRDMMKHRADLSLVAAGAAVAQGVDSVAKELITSYTTVKKQAERLWSKGKRS--HMKTELEPGE----------AGAGGAEDWDLELTLTVVEFQRTKLERALLAGKRNGADALKKREAMISGVLRIINGTTRQHHAAMLEAEARRAXXXXXXXXXXXXXXGEWGAMWGWKSEGAAAYETRLRLLTLQGQWLEM-EGRVMAEGTTAAGGERESTPSSDLAGASNSLTAALASLQEAANLGDRLISAPVGVDAAVGVSTASPSLAECVKTTGIV---------------EATGEATLRLAKLCEKLAVG--DGIVEAGVGGGXXXX----EELAALAAEHYLRAMAMGSKDARDHFPRVLFLVGE----FPEACDAFSAR------ASSVPEWTFLRWISQMLGVTGRREGPAVIKVLEGVAKVYPRALFYPLLMTKASSSSRRTEGDDDDAKLCRLTAMVFDPSAEAFAEALRGLHDPQLRFCDDMKRVVELLRTGQ--KDLALKTYLKMRSECLVTV----GLKTNSKIGQLNREFAKQYGPEVDQALGKDGQRLDLAKAKKCL-DKGLIWAGRARAKKTGGTARLSHLSSWMSDFDSNV----HTLEVPGQYG---GISHGPPRVSHHSRIISFGSELLVMKSLRMPKRLTMHANDGKDHMFLVKGGEDLRVDERVEQLFEVMNAVMAASPGCRRARLTNKTYQVVPVTPDIGMIEWVQDTRPLKSVIEEGLG-----------------------------QRLDGHEAHALFIKWLRGDVGAAKFHAMFKTASRAEVEGAFAKRCASVLPTDALRRHLIAMAPSPEAFLTCRGGFARSLATLSACSYMLGIGDRHLDNFLLDTTTGTVVGIDFGAAFGVGTTQLGVPELIPFRLTHQFQTLLQPLDSVGLLRGYMISTLQALRGSQEVLLATLDVFLNEPVIDWLSHGAGDRQKGRGKGTYEXXXXXXXHRPGTGSSQPRPRSETGRKIQNSRRKLQGENPVPVLVDDLNQNLIVKKFGSLKKLEEIAWGHSGK-RFRAKPEL----REG---------LLSVEDQVDCLIDLATDPDVLGRQWIGLTTWV 5165
            E  Q+   PAW   +   LAD                      GL RN RL   Q  LN P    L P+       K +V +TD  L P          P   HYLL D A   L    +      R A              RC        A   DD      RR+   L +       L+S+   PP  TP         S L+                 H  A     +D   A  P               LG           +   GG  ARA+   G QA    +  +N L +L  A  + V  +  P    LA+++ ++ L   Q  +   +E   E+  + L  +  L         + G    G    EQ           C  +    +   +  +++S G    K+G+      +  V    G +  +L       SRR    +V  +  +Q          L  LA++     +  LE       + PFLP  L++T   F+RG+        W P  Q   + L+R  +  M E                +Q        +G +L E  D  HSL+ LA     T    RE V+  L+ L+                   SS  R S    +G   ++  + L  E    +   L+RGLSDPDD                   +     A +GIRRRL+  F G LS          P  +L  ++ N+       ++L    YL+L  +  +P     +F + LA  +F D+P++              P    M P FSF   ++L SQ       G  R  G+     T S +V     G +R+TQ      W+ TQ  GG ER                          +A  + ++   L    A GP               R +M PP PR    + +G       +AG+ G                         +                 V+YR+YR GELPDIQI   DI++PLQ L + D  +AR +F  +F  +YA+    SG G                          MR               ET +VA +LAA RAA                    +T GR+G                        +  G +  AE   + +    +GG  +L   L A+S + S NF   IL+LEE +IR  G E V                    +  GR G  +M     G G   EG       W++L+RLY  LGE DVV G+  R                 S +  TR A+  E  G +  AL+ Y +L+  +              K+ +          W   + ECL +LG+W  +++++ A++   ++  S      +A +      KLW  +      R+G        L   + SL+   S        +E  R     VL+    LL +A+           +  +R +WL +  P  LA      G    A+  +++ Y  F  +W  LHP A +ARR QL GL+  VE+ED L  +        RTL    PS  S PSS+         SP     + LL R W+  P+  ++   P+ W  V         T  E  G    +     +   D +R+   H       AA AAV +  + V              A + W K K +  + K  ++P E           GA  ++ W  ++ L  VE  + KL +A+  G R GA       A++  VL       +     ++  E R +                          G+ +    +  +  + +W E+  G V        G ER+           ++   ++A        G        G + A   S     L E  +    V                       + A+ C++L     + + + G  GG          L A A E YL A+     +A    PR++ ++ E    +    +A  AR      A SVP W FL   SQ+L      E  AV+ VL  +A+ YP+ALFYP  + K   +S       +  +L +L      P+ E F E +  L    + +   + R+  LL+ G   ++     Y   R     +     GL T    G +  E AK+  P+    LG+ G+ L+    K+   D  L   G  +  +     R+   S W++ +        H +E+PGQY    G    PPR   H  ++S    +    S ++P+++ +HA++ K + FLVKGGED R DER+E+LFE MN +MAA   C + RL  +T++++P++P +G+IEW  +T  L+  IE+G                               +R D  E  A     L   V    +H  ++T S ++     A   A V P + LR++L+ +APSPE FLT R  FA+SLA  S CSY++G+GDRHL+NFLLDT +  VVGIDFG AFG+G + L VPELIPFRLT Q   +L+PLDS GLL+  M   L ALR  +++LL T+++F+ +P+ DW   GA   Q+   +           +  G  ++Q     E  RK+   R KLQG NP  VL+ DL  N  V ++GS   + +   G  G+ R R  P      +EG         +L+V +QV+ LIDLATDP+VL RQ++GL  ++
Sbjct: 2767 ENGQQRERPAWMLALETALADGDEGGRGXXXXXXXXXXXXXGEGLHRNARLFVLQACLNEPTASILAPFVNEGLGNKVLVAVTDLLLYPPSSPSSPCPSPSACHYLLCDVADTFLSAWQQ-----YRPAPPSSPDSFTPTALDRCTAVLCYFLAHAHDDISSLVTRRNLYYLSS-------LLSLWIAPPSITPSXXXXXIPFSSLLLQ-------------RRHHHALRRAFEDALFAEPPSLXXXXXXXXSSSSTLGPFGTLLHLLSTQTEKGGGKARAKGGEGIQAVRRRITAVNALALLVTAGRINVLPARHPT---LAQSLHRLLLQAMQAESVLVYEVAAEVAGLLLRFLYPLSPEGEEEIGEEGKARAGGYHEEQRPHPLVPNVDPCAQNLPRQVRDLLLTRILSTGDVDAKKGEQGPRSVVALVGRGKGGFKELLDGRQGRESRRFWLAVVAMLRKVQHIPPKAMANLLEALAESDAPHDLTFLE------EVLPFLPPCLVDTSRAFLRGA--------WRPGVQYGGICLLRRGLKEMMEGGREGVXXXXXXGRGGRQEKVARILLVGGLLDERGDVPHSLITLAVNPRITDLEVREEVFGLLMDLHRFFSSSPPSIXXXXXXXXXSSHHRASPPGQEGKEGKALSS-LQEEAQRRLSLCLIRGLSDPDDRXXXXXXXXXXXXXXXXXXEERMVPAEKGIRRRLI-AFWGSLSSTPSSLPPTSPVTKLQSLLHNLHHPSNPSAFLPTTTYLLLSLSLASPAASSPLFPQPLANVKFTDLPLSFRTSLPSXXXXXXXPAISTMSPLFSFRRTSALLSQATQREGRGRGRERGN-----TASGEV----TGWIRATQGGSQGAWALTQ-EGGEERAEKGSGGRGGSGIGREGGHQFTTVSGVASQAGSKESALMVRKAGGPEXXXXXXXXXXXQEGRGLMPPPPPR----KSKGLTWEDKMRAGLEGXXXXXXXXXXXXXXXXXXXXXGGRPV-----------------VLYRRYRKGELPDIQIPASDIIQPLQLLCLRDAAMARTLFAAIFPPLYAEA---SGGGRVXXXXXXXXXXXXXXXXXXXXXXGSMR---------------ETELVAGVLAAYRAA-------------------MDTEGREG---------------------GEEDGEGMEVEAEEGVEEKEDRKEGGGGMLPVHLTAESGLQSLNFQAAILVLEEELIRREGMERV--------------------AGRGRRGGGEM----TGTGRKKEGSSSTLAIWVELTRLYRVLGEGDVVRGLVERIXXXXXXXXXXXXXXXXSAMTRTRLAVASESEGHWEKALEQYAKLLE-FSEKPXXXXXXXXXXKEVVAL--------WHSRRRECLRRLGEWTQLQDEIEAVVQPAKQHSSGASYSIEAMSINGIYDKLWSLEEVWQGGREGGGARGGDVLAMWIESLVHRFSAACLGKEEQEKARGETSSVLNLQEALLLVADD----------ADAARRQWLASAHPSLLASLHFFLGNQVHAQAYVEQAYRTFIRSWKGLHPLAGAARRAQLEGLEYLVEMEDALRIS--------RTLP---PSSSSFPSSS------LTRSPSSWEGKRLL-RGWQLAPAPVMEKDPPLVWANVGMLREWSLSTPRERDGHAPSQDMDEEEDMRDFRRERYWHHLTFWQRAARAAVKEWGEGVV-------------AMQCWEKAKVAFHNWKFLVDPEEEEDSERIETLVGAMFSKVWAAQIRL-FVELDKWKLRQAVEEGGRGGA-------AVVERVL--WGRAAKAIRQRLMSLEGRES--------------------------GSQSRRAWMEAMQFKAEWFELASGFVTKRIKWEVGQERKR----------DAFLCSIAKSYWKVWRGQETQDETAGFNVA---SLRQDWLHETYQAYRAVMTEEKEERRRWGRGTRTVARRAAKFARFCDRLVRERREDMAQEGKQGGESEHLFSPAALTAQAMERYLEALTWCPAEAPAALPRLVDMIRESHDPYEPQSNARVAREVLQKFAGSVPAWIFLPAASQLLAGLDAPEAEAVLPVLLRLAQAYPQALFYPYRVAKPGFTSMALAYAQELDRLLQL------PALERFVENVAMLVSFPVAWRGILGRIRSLLQVGDVPQEEVQHVYAAWRETLTESSAEDRGLYTR---GWMKNEVAKRLDPK----LGRRGEHLNRQTLKEVASDLALNVQGTPKHTQE----RIERYSLWLARYRMECGDGDHKIEIPGQYDRRYGRWESPPRPDLHVHLVSIDPLVETRTSKQLPRKIVLHADNEKSYPFLVKGGEDCRNDERIERLFESMNGIMAADTACAQDRLECRTFKIIPLSPSLGLIEWCSNTIVLRLAIEKGSRSRGAQLAVSEHDHRGKRGHPPRTSERFKEEREDSFEVLAHDYAQLYPSV--MDYHTGYRTLSPSKAGEKLAAFHARV-PWNGLRQYLLLLAPSPEVFLTLRSTFAKSLAAFSICSYVVGVGDRHLENFLLDTRSACVVGIDFGVAFGIGASALPVPELIPFRLTRQMLNVLRPLDSEGLLQIAMQKVLHALRRGEDLLLPTMELFVRDPIWDWA--GAPGGQEADKESAPLSAGDRRKNVTGNSTTQ----EEAYRKVGLVRAKLQGANPRQVLLHDLATNPHVARYGSFDGIRQAVEGQLGRARTRWCPSRGEKGKEGERGKGREMDMLTVGEQVEVLIDLATDPNVLMRQYVGLAPYM 5281          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: A0A6H5KTW4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KTW4_9PHAE)

HSP 1 Score: 409 bits (1052), Expect = 6.730e-122
Identity = 260/555 (46.85%), Postives = 331/555 (59.64%), Query Frame = 0

Query: 4235 GAGGAEDWDLELTLTVVEFQRTKLERALLAGKRNGADALKKREAMISGVLRIINGTTRQHHAAMLEAEARRAXXXXXXXXXXXXXXGEWGAMWGWKSEGAAAYETRLRLLTLQGQWLEMEGRVMAEGTTAAGGERESTPSSDLAGASNSLTAALASLQEAANLGDRLISAPVGVDAAVGVSTASPSLAECVKTTGIVEATGEATLRLAKLCEKLAVGDGIVEAGVGGGXXXXEELAALAAEHYLRAMAMGSKDARDHFPRVLFLVGEFPEACDAFSARASSVPEWTFLRWISQMLGVTGRREGPAVIK-----------------------VLEGVAKVYPRALFYPLLMTKASSSSRRTEG----DDDDAKLCRLTAMVFDPSAEAFAEALRGLHDPQLRFCDDMKRVVELLRTGQKDLALKTYLKMRSECLVTVGLKT-NSKIG------QLNREFAKQYGPEVDQALGKDGQRLDLAKAKKCLDKGLIWAGRARAKKTGGTARLSHLSSWMSDFDSNVHTLEVPGQYGGISHGPPRVSHHSRIISFGSELLV 4755
            G  G ++WDL+LTLT VE  R +LER  LAG R GAD  +K+   ++  L+++ G   Q   AM     +RA                WG   GW+++GA A+ETRLRLLTLQGQWLE +G ++AE                   A+ S  A+LASL++A  LGD L   P     +    ++  + +    T G VEATGEA LRLAKLC+ ++ GD  +    G G    E+LAALA + YLRAM +GS  ARD FPRVL+L G+F +A  AF+  AS VPEWTFLRW  QMLGVTGR EGP VIK                       VLE VA  YPRALFYPLLMTKAS+++R  EG    DDD  KL RLTA+  DPSAEAFAEALRGLH P++RF D MKRV  LL   Q DLA   Y K+R++CLVT G KT + K+       +L  + ++++  E+D+A+GKDG+ L+  +A++ L +G  W  +   KK+    +LS  S+WMSDFDSN H LEVPGQYGG+S  PPRVS HSRI+     L V
Sbjct:    3 GTDGDDNWDLQLTLTTVELNRVRLERLRLAGNRTGADLARKQADYVARTLKVLGGCISQQQQAM----GKRASGDTRG----------WGEGEGWETQGAKAHETRLRLLTLQGQWLETKGHLLAENXXXXXXXXXX--------AAESFRASLASLEKATELGDSLTVRPPRSKLSPTPGSSDGNASGTTPTPGAVEATGEAMLRLAKLCDTMSAGDSTMSDVRGRGR---EDLAALAVKEYLRAMGVGSAGARDSFPRVLYLAGKFSKARAAFADGASVVPEWTFLRWRWQMLGVTGREEGPVVIKARLVCGKLKEKNGRVMTFRILLAVLERVALAYPRALFYPLLMTKASAAARNGEGGAGADDDGRKLSRLTALTADPSAEAFAEALRGLHHPEMRFSDGMKRVCGLLNKSQIDLARVAYRKLRNDCLVTTGRKTGDDKVVPQTVCVRLVSQMSERWR-ELDKAMGKDGELLNDREARRFLFQGRKW--QLEVKKSS-RLKLSGFSTWMSDFDSNRHKLEVPGQYGGMSAAPPRVSLHSRIVRQERALFV 528          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: A0A6P8J6V1_ACTTE (DNA-dependent protein kinase catalytic subunit n=2 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8J6V1_ACTTE)

HSP 1 Score: 445 bits (1144), Expect = 6.830e-121
Identity = 275/711 (38.68%), Postives = 395/711 (55.56%), Query Frame = 0

Query: 4472 LAAEHYLRAMAMGSKDARDHFPRVLFLVGEFPEACDAFSARASSVPEWTFLRWISQMLGVTGRREGPAVIKVLEGVAKVYPRALFYPLLMTKASSSSRRTE-GDDDDAKLCRLTAMVFDPSAEAFAEALRGLHDPQLRFCDDMKRVVELLRTGQKDLALKTYLKMRSECLVTVGLKTNS------KIGQLNREFAKQYGPEVDQALGKDGQRLDLAKAKKCLDKGLIWAGRARAKKTGGTARLSHLSSWMSDFDSNVHT--LEVPGQYGGISHGPPRVSHHSRIISFGSELLVMKSLRMPKRLTMHANDGKDHMFLVKGGEDLRVDERVEQLFEVMNAVMAASPGCRRARLTNKTYQVVPVTPDIGMIEWVQDTRPLKSVIEEGL--GQRLDG--HEAHALFIKWLRGDVGA---AKFHAMFKTASRAEVEGAFAKRCASVLPTDALRRHLIAMAPSPEAFLTCRGGFARSLATLSACSYMLGIGDRHLDNFLLDTTTGTVVGIDFGAAFGVGTTQLGVPELIPFRLTHQFQTLLQPLDSVGLLRGYMISTLQALRGSQEVLLATLDVFLNEPVIDWLSHGAGDRQKGRGKGTYEXXXXXXXHRPGTGSSQPRPRSETGRKIQNSRRKLQGENPVPVLVDDLNQNLIVKKFGSLKKLEEIAWGHSGKRFRAK-PELREGLLSVEDQVDCLIDLATDPDVLGRQWIGLTTWV 5165
            +   + L+AM   S++AR  FPR+L LV  +P+  DAF  +AS VP W F+ WI+QM+ +  ++EG AV  +L  +A  YP+AL YP  ++        ++ G  +   + RL A++  P    F  +L  + +P L F D  K +  L+       A + Y+ M          K  S      ++G   R FA +Y  +V +  G+DG +L     K   DK   +  R + K       L   S W+  F S+ +T  LE+PGQY G   G P   +H++I  F   +LVM S+R PKRLT+  +D KD+MFLVKGGEDLR+D+R+EQLF +MN ++AA P C++  L  +TYQVVP+TP +G+IEWV +TRPLK  I + +  G+R      +   L  +WLR        +K+H M+  ASR EVE  F ++ A V P D LRR    ++ SPEA+L  R  FAR  A L  C Y++GIGDRHL NF++D  TG +VGIDFG AFG  T  L +PEL+PFRLT Q    L PL   GLLR  MI TL+ALR + E+LL+T+DVF+ EP++DW ++ A ++ K  G  + +        R                K++ +++KL G NP  +  +++      +K+  +  +E +  G      RAK PE+    L VEDQVDCLID ATD ++LGR + G   W+
Sbjct: 3452 IVVRYVLKAMTYCSEEARQRFPRLLQLVELYPDTLDAFIKKASVVPCWMFIGWINQMVALMDKKEGRAVHGILNEIATKYPQALCYPFKISSXQFVFDNSKTGKTNKEAVDRLHAILEIPLLNDFIASLEQMQNPXLAFEDWSKNMRPLIEDKNIAAAEQCYMXMLKNLFGDERTKDRSLKKRGQEMGTYRRRFAHKYAADVKKGFGQDGSKLKTISLKDFNDKVKGFINRMKYKPENAPGNLKEYSPWLLSFQSSNYTQRLEIPGQYTG--RGKPLTEYHAKIAGFDERVLVMTSIRKPKRLTIRGDDEKDYMFLVKGGEDLRLDQRIEQLFCLMNDILAADPACKQRNLHLRTYQVVPMTPRVGLIEWVTNTRPLKEFISQAMTPGERQQRVTDKPRELHDRWLRKYANVDDNSKYHVMYSKASRTEVEFDFHEKQAFV-PWDLLRRAFEQLSASPEAYLMLRSHFARMHACLCICQYLIGIGDRHLSNFMVDIKTGGMVGIDFGHAFGSATQFLXIPELMPFRLTRQIVNFLLPLKESGLLRSTMIHTLRALRENHELLLSTMDVFVKEPLLDWKAY-ARNQAKMHGLNSEDEEDVVWYPR---------------EKVETAKKKLCGANPAYLTRNEIRLGHKNRKW--IDAMETVCIGDPNVNIRAKKPEVG---LPVEDQVDCLIDQATDSNILGRTYHGWEPWM 4138          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: UPI001BB1774A (DNA-dependent protein kinase catalytic subunit-like isoform X2 n=2 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB1774A)

HSP 1 Score: 439 bits (1130), Expect = 2.960e-119
Identity = 542/2149 (25.22%), Postives = 864/2149 (40.20%), Query Frame = 0

Query: 3081 LLVTSIEAMQLADASRRGRFLAMLADAFPRGH-VPALEVQDLFTRLQPFLPSLLLETEEEFIRGSKQAGAPSDWVPVAQLSALRLVRALVPTMTEKQLGAILGEDDAHSLVGLATPSTYPACREVVYDTLLYLYTSSQQRFSKETDDGNGARSGGAPLNREKAEAVRCALLRGLSDPDDEGMTFDDSGADASDRGELDDSARRGIRRRLLDVF--EGGLSDEVPGDRLLYIMRNMFDARTARSWLRFAPYLMLRPAEKAPDYDGLMFKKRLAACEFVDMPVAGGIGTLDARPMEPTFSFASL-QSQXXXXXDEGARNYGDGGSYGTFSTDVDVRQAGMVRSTQA-YLWSQTQGPGGAERPRNIAFLSQTQSRMLAAGPVDSSAARS----VMGPPAPRLPGVRPRGRGVGVNSQAG-------------IGGVGKRFRQSAEGSVSSLGHGESHAMQLRVAAKRERDKAARAKRAVIYRKYRDGELPDIQIKLGDILRPLQHLSMLDGGIAREVFVQVFKAVYAKLDSGSGSGSPPHGALKRGGVDGEDDEWEASEGTGMRAVSLLLQRMLS-ATKTETGVVACLLAACRAAAAAGGCDIEAPLYENVVGGRETSGRKGRGGGSSQPRRGRLMTPSSGTAATINQSGKKTGAESQPRASEPDGGAIVLNPKLVADSAIASFNFHGGILLLEEAIIRSGRESVXXXXXXXXXXXXXXXXXXXXSSGGRAGTIDMHPGEDGLGASVEGQQDHWLQLSRLYAALGERDVVVGV-SARASRLEGTRQALDCELSGDFNGALKMYRELVRRYDSAEMDDDEGADAAKDAMIAASETELLDWDMHQLECLLQLGDWEGVREQVLAILPEDEEDESRGGDADAATECRRKLWKADPSDRDGTLPYLVRSLLDDESRREEHLRDFLEDVLDPGAPLLRLAESYAPAGASGVSSSCSRLEWLEANMPGELARAFLLEGELGRAEQNIQRCYERFQETWAALHPCADSARREQLRGLQVAVEVEDFLLFADQRATTGHRTLTRTAPSPRSIPSSAWRSGTTAAVSPFCDRLRGLLERWWRQVPSRTLDPVAWWRVVRTHR---HDCFTAIEGFLEDEGGLSNQARLDCKRDMMKHRAD--LSLVAAGAAVAQGVDSVA-KELITSYTTVK--KQAERLWSKGKRSHMKTE---LEPGEAGAGGAEDWDLELTLTVVEFQRTKLERALLAGKRNGADALKKREAMISGVLRIINGTTRQHHAAMLEAEARRAXXXXXXXXXXXXXXGEWGAMWGWKSEGAAAYETRLRLLTLQGQWLEMEGRVMAEGTTAAGGERESTPSSDLAGASNSLTAALASLQEAANLGDRLISAPVGVDAAVGVSTASPSLAECVKTTGIVEATGEATLRLAKLCEKLAVGDGIVEAGVGGGXXXXEELAALAAEHYLRAMAMGSKDARDHFPRVLFLVGEFPEACDAFSARASSVPEWTFLRWISQMLGVTGRREGPAVIKVLEGVAKVYPRALFYPLLMTK-----ASSSSRRTEGDDDDAKLCRLTAMVFDPSAEAFAEALRGLHDPQLRFCDDMKRVVELL--RTGQKDLALKTYLKMRSECLVTVGLK-----TNSKI--GQLNREFAKQYGPEVDQALGKDGQRLDLAKAKKCLD-KGLIWAGRARAKKTGGTARLSHLSSWMSDFDS-NVHTLEVPGQYGGISHGPPRVSHHSRIISFGSELLVMKSLRMPKRLTMHANDGKDHMFLVKGGEDLRVDERVEQLFEVMNAVMAASPGCRRARLTNKTYQVVPVTPDIGMIEWVQDTRPLKSVIEEGLGQRLDGHEAH-------ALFIKWL------RGDVGAAKFHAMFKTASRAEVEGAFAKRCASVLPTDALRRHLIAMAPSPEAFLTCRGGFARSLATLSACSYMLGIGDRHLDNFLLDTTTGTVVGIDFGAAFGVGTTQLGVPELIPFRLTHQFQTLLQPLDSVGLLRGYMISTLQALRGSQEVLLATLDVFLNEPVIDWLSHGAGDRQKGRGKGTYEXXXXXXXHRPGTGSSQPRPRSETGRKIQNSRRKLQGENPVPVLVDDLNQNLIVKKFGSLKKLEEIAWGHSGKRFRAKPELREGLLSVEDQVDCLIDLATDPDVLGRQWIGLTTWV 5165
            + +T +  + L+      RF+  L    P  H  P  E   + T     + +  +E + + I         +D     QL++L+LV  ++P +   +L  +L          ++ PS  P CR+ +Y  L+++Y + ++   ++ +D + A S             + +LL+GLSD ++                         +R R+ + +  E  L  E  G RL+ ++R M+   T   +L +A  L+L    K+PD+   +F   L+AC+F ++ +        A    P F    L QSQ        +++ G G               G +R+TQA   ++ TQ    A R     +L+ +Q          SSA+ S     MG    R    +   +  G     G             I  + +RF +      +      +   QLR   ++ + +  R  +  +YR+YR G+LPDIQIK  DI+ PLQ L++ D  ++R +F  +F+ ++A++                      DD  E   G  ++ +   L +ML+ + + +   ++C+L              E   Y +                                                          + L+P  ++ S++ S     GI +LEE +I+   +                       S  RA T      ++           +W++L+RLY A+GE DV+ G+  +R    + T +A+  E  GD+  ALK+Y E +    S+E   D          +   E +   WD  +L+C  QL  W+ +             D+  G + +  ++    LW  D   ++  LP ++RS L  +   E    D L   +D  A                   +  R   LE+    ELA   +L+ +  RA+  +      F + W+ L P   S+R  +L+ +Q   E E FL  A++  + G             + SSA R                LL  W R +P    DP++ W  V  +R    D    I G     GG + +   D K D +  R    LS+  A +A  Q    VA K L +S+  +   +  +  W+    S ++ +   LEP E      +   LE    + E Q  + +  LL   R                  I+   T +  A  L+   R                         K   AA  + +      Q  + E   + + E       + ES  SS L  ++   + A A +    +  D+L+      +         PSL E   TT ++                                              L+AM + S  AR HFPR+L +V ++PE        ASS+P W F+ WISQM+ +  + E  AV ++L+ +A+ YP+AL YP  ++         S    +  D   ++  + + V     E    AL+ L  P   F D ++ +   +  RT       + Y K+  +   + G +     +N  +  G   ++  ++Y    D   GKDG +L     K  +  K  I+      K   G   L   S W + F S     LE+PGQY G     P  ++H +I  F   ++ + SLR PK + +  +D  D+ FLVK GEDLR+D+R+EQL+ +MN ++A    C +  LT +TYQV+P+T  +G++EWV++T  LK  I   + +    H A+        LF +W+      R       + A F+ A+  E   AF +R A V P D LRR  + ++ SPEAFLT R  FARS A L  C Y+LGIGDRHL NFL+   TG +VGIDFG +FG  T  L +PELIPFRLT Q   L++P++  G L+  M+ TL+ALR   E+LL T+DVF+ EP +DW   G  D+Q    + + E       +          PR     KI  ++ KLQG NP  +   +L      K    LK L+ I     G R   + +     L VEDQV CLID ATDP +LG  W G   WV
Sbjct: 2395 IFITCLHKIHLSYPVFTDRFMNKLLFMLPSIHGQPKTECLQIITARIEHIDNAFIEMKNKNILALLTHKDEAD-----QLTSLKLVDGMLPKLQPSELLYLLPAIKDF----VSHPS--PTCRDAMYSILMWIYDNYREDDRRDGEDADKALS-----------IAKDSLLQGLSDQENY------------------------LRLRVSNFWSHETRLPKETLG-RLIAMLRCMYSPSTENRYLGYATNLLLEMTSKSPDFKREIFDHPLSACKFEEVQIDHSWRQRHAAISTPKFVETQLGQSQGSMRGSPSSQSMGMG--------------RGQLRATQATQQFAPTQENAAASRKNAYNWLTGSQDTFADYSSSSSSASESSLLFTMGSLPKRTDAFKSSKQDTGAGGSRGNDTRKAPSEGEKEIQRLKRRFLKDQSSQSAFFAKRATRQKQLREEVQKSQ-RERRFAQVTMYRQYRTGDLPDIQIKHSDIIAPLQALALRDSTLSRLLFCALFRGIFARIG---------------------DDCTEREAGIHVKEIETHLNQMLNESNQFDPAFISCIL--------------EIGFYHSKQ--------------------------------------------------------LTLDPAAISQSSLTSRQLLIGIRVLEEVLIKKSWDD--------------------ERSRKRARTSRPEQSQE---------MKNWIELARLYKAMGEFDVLRGIFGSRIETKDVTHKAIQAESRGDYEKALKLYSEAI----SSESTQD----------VTQEEEDF--WDEARLQCCNQLTKWDNMESFAT--------DQIEGKETNDFSD----LWN-DSYKQEIYLPCMIRSKL--KMLLEGSGDDSLTTFIDSSA------------------KNPERRAILESRYTEELAALSILKEDYNRAKYYLANSTSSFLQEWSGLGPLMLSSRLSKLQDIQKLTEAEQFLQLANKPFSEG-------------LKSSAIR----------------LLSNWSRNLPDPKRDPISVWDDVTQYRCLYMDKLCNILGDSPGGGGDTMETMGDGKFDDLVRRKKVLLSMKMADSACQQSNFFVATKHLKSSHRDLADFEDLKPAWTHSYVSMIQRKALLLEPYERITSLLKT--LEQLGKLPESQGLRSDLVLLCNHR------------------ILTSNTYRSMATALQDGKREILERLSDEGKLD------------KLLEAAKCKEKKHEEVCQSLF-EKSKKYLDEAVVTIKKDPESNSSSHLLSSTERESMAKAHMT-LVSFSDQLLRQKESENCD---PDCLPSL-ETYPTTLVIST--------------------------------------------LQAMKLSSTKARQHFPRLLQIVEKYPETMKTLVKEASSIPSWMFIGWISQMVALLDKPESLAVQEILKTIAEDYPQALVYPFKISSEDFKLGDGSPEERQRKDAVKEIDNILSKVS--LVENLTLALQRLTHPGFLFKDWVEGMKPFMDKRTHNPSAIKREYQKLYQQLFESGGRRGEPSSSNQTVEAGPYWKKVGREYQKRFDSEFGKDGSKLASMDYKMLVSVKQKIYDKMNPGKDDPGN--LKEYSPWFTAFQSLQGDELEIPGQYDGSKK--PLPAYHVKIAGFDETVITLPSLRKPKCIKIRGDDETDYPFLVKVGEDLRMDQRIEQLYCIMNGILAQDAACSQRGLTLRTYQVIPMTSRLGILEWVKNTTTLKDFITRAMSET--EHNAYRSAAGPGTLFAQWIKKFSNDRNPNEVQMYAAAFQRANETETVKAFREREAKV-PWDLLRRGFMELSASPEAFLTLRAHFARSHAVLCICQYILGIGDRHLSNFLISLETGGMVGIDFGHSFGSATQFLPIPELIPFRLTRQIVNLMRPMEIEGDLQSSMVHTLRALRQDHELLLNTMDVFIKEPSLDW--KGFADKQAKTLEMSEEDRKDISWY----------PRE----KIDLAKMKLQGANPTFITKKELEMGHS-KNPDVLKALKAIC---GGTRNHKRAQCGTKGLRVEDQVACLIDQATDPHILGITWQGWEPWV 4172          

BLAST of mRNA_A-nodosum_M_contig1084.3.181 vs. uniprot
Match: UPI001CF13E17 (LOW QUALITY PROTEIN: DNA-dependent protein kinase catalytic subunit-like n=1 Tax=Acropora millepora TaxID=45264 RepID=UPI001CF13E17)

HSP 1 Score: 436 bits (1122), Expect = 2.520e-118
Identity = 273/725 (37.66%), Postives = 402/725 (55.45%), Query Frame = 0

Query: 4472 LAAEHYLRAMAMGSKDARDHFPRVLFLVGEFPEA--CDAFSARASSVPEWTFLRWISQMLGVTGRREGPAVIKVLEGVAKVYPRALFYPL-LMTKASSSSRRTEGDDDDAKLCRLTAMVFDPSAEAFAEALRGLHDPQLRF---CD-DMKRVVELLRTGQKDLALKTYLKMRSECLVTVGLKTNSK-------IGQLNREFAKQYGPEVDQALGKDGQRLDLAKAKKCLDKGLIWAGRARAKKTGGT-----ARLSHLSSWMSDFDSN--VHTLEVPGQYGGISHGPPRVSHHSRIISFGSELLVMKSLRMPKRLTMHANDGKDHMFLVKGGEDLRVDERVEQLFEVMNAVMAASPGCRRARLTNKTYQVVPVTPDIGMIEWVQDTRPLKSVIEEGLGQ-RLDGHEA-------HALFIKWLRGDVGAAK--FHAMFKTASRAEVEGAFAKRCASVLPTDALRRHLIAMAPSPEAFLTCRGGFARSLATLSACSYMLGIGDRHLDNFLLDTTTGTVVGIDFGAAFGVGTTQLGVPELIPFRLTHQFQTLLQPLDSVGLLRGYMISTLQALRGSQEVLLATLDVFLNEPVIDWLSHGAGDRQKGRGKGTYEXXXXXXXHRPGTGSSQPRPRSETGRKIQNSRRKLQGENPVPVLVDDLNQNLIVKKFGSLKKLEEIAWGHSGKRFRAKPELREGLLSVEDQVDCLIDLATDPDVLGRQWIGLTTWV 5165
            +   + L+AM  GS  A   FPR+L +V  +P+     AF  +A+ VP W F+ WI+QML +  +REG AV  +L  +A+ YP+AL+YP  + ++        EG      +  L + +  P    F  AL  L DP++ F   CD  M++++E         A++   K   + L   G +  +         G + + FA+++  EV +  GK+G++L   K  +  ++   W    R  +  G+     + L   S W+  F SN   H LE+PGQY G     P   +H +I  F   + V+ S+R PKR+ +  +D K++ FLVKGGEDLR+D+R+EQLF +MN VMA+ P C +  L  +TYQV+P+TP +G+IEW+Q+T+P K V+++ + Q  LD +         HA +I+  +GD+   K  +  M+K A+R E E +F ++   V P D LRR  I +A SPEAFL  R  FA++ + LS C Y+LGIGDRHL NF++D  TG ++GIDFG AFG  T  L VPEL+PFRLT QF  L+ PL   G L+  M+ TL+ALR + E+L+ T+DVF+ EP +DW    A  + +  GK T +          G  +  PR      +KIQN+++KL+G NP  V+ DDL   L  K     K +E +  G +    RA+    E  LSVE QV CLID A DP++LGR + G   W+
Sbjct: 3452 IVVRYMLKAMCYGSDQALQRFPRLLQIVEMYPDTDTVQAFITKAAVVPSWMFISWINQMLAILDKREGKAVQAILWEIAQTYPQALWYPFKISSEQFVFDNSKEGKTSQDAVKSLQSALESPLMNDFISALEQLTDPEMSFKDWCDGSMRQLLENKDKRTDAQAIRDCYKSMFQNLFESGTRAQNTSQIRGNDFGPVRKRFAQEFSGEVQKLFGKEGEKLIGLKVNQFTNQ---WRAVHRKMEEKGSRSRPVSNLKDYSPWLFHFQSNNYTHALEIPGQYTGREK--PLPEYHVKITGFDERVKVLISIRKPKRIIIRGDDEKEYAFLVKGGEDLRLDQRIEQLFGLMNDVMASDPACSQRGLRLRTYQVIPMTPRVGLIEWIQNTKPFKDVLQDAMTQTELDNYRGNDGPRKRHAKWIQKFKGDIANPKSFYGEMYKKANRTETERSFQQKVGQV-PWDLLRRSFIKLAASPEAFLILRSHFAKTHSCLSICQYILGIGDRHLSNFMVDMETGEMIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPLKESGTLQSVMVHTLRALRSNHELLVNTMDVFIKEPSLDWQVF-ARKQARAHGKNTDD----------GDVAWYPR------QKIQNAKKKLEGFNPAYVMRDDLA--LGHKSTSWYKSMENVCLGDAKTNVRAQEP--ESGLSVESQVACLIDHAIDPNILGRTYDGWEPWM 4149