mRNA_A-nodosum_M_contig104.85.5 (mRNA) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: D7G588_ECTSI (Histidyl-tRNA Synthetase Similar to Histidine Ammonia-Lyase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G588_ECTSI)

HSP 1 Score: 1163 bits (3009), Expect = 0.000e+0
Identity = 639/871 (73.36%), Postives = 716/871 (82.20%), Query Frame = 1

Query:  298 MAPEGSAATVVLVGARKVLPSDVLLVAVHGARIDLNQAALEKACPRTSQASSEGDASYDASVGDAEFSPDCAMSETATRAALFARIVSLMNGRSGLRPDVVRFLADLLNNGIIPLLLAQREGGEGAAVAKAVLGAGLCMFKGQIRPLSEVASECEISPLEALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYDARPHRGQMECAERLRLMLEGSKSCNSKKSGE-VDAKAISCIPQYHGPARDAISAVCRSIMTEFNGSDAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDL---DGVLRELQLALAEELSLSIGMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLA-GVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLPIGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFSGS-EVPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASV 2892
            MA EGSAA  V VGARKVLP DVLLVAVHGAR+DL+ A+L+KACP  S  S  GD ++  S G  E   + + SETA RA +FAR+VSLMNGRSGLRP+V+  L D+LNNG+ PLL   ++GGEG ++A+AVLGAG C+FKG++RPL EV S CE+SPL  LT +EG+TL LGRFD+IG AA+  AAA+NAIEIADGVAA+TCE T ANT+PF+A N+D RPHRGQ+ CA+RLRLML+GSKS NS K    VD +AIS IPQYHGPARDAI+A  RSIMTEFNGSDAGPLG G GCV+PRD KQT L   +L+EAVR+LTSG R+R   L+G      + NGA   G   D+   +GVLRELQ ALAEE++LS+ +LR                               + A  G  DT+G + AQIAKIE KRXXXXXXXXXXXXXXXXX KAAGLGVGCAEL+QRV+PAG   A G +EI+  IDPFRTG+QAMGPFL A+AE+LS GGAQRKPKIAKGARDFLPEQMR+REQAF AIR VFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHG+GNIKR+HIAKVYRRDQPNVQLGRFREFYQCDFDIAG+YG MVPDAEVLSVACEILSSLPIG FQIKLNHRRLLDAILDICGCPPEKFR+ICSAIDKLDK++W+EVRQEMVEEKGLAPD+ADKIGGFVNGNAGG KELWAKL E+K FGDHPDA AAMSELELLFKYLDAMGTL+YVSFDLSLARGLTYYTGLIYEC+VVDGG+RVGSIAAGGRYDNLVGMFS S + PCVGVSIGIERVLTIMEKRLQEE+ + G   NVQV  +SV
Sbjct:    1 MASEGSAALAVSVGARKVLPEDVLLVAVHGARVDLHPASLDKACPVPSTPSRGGDKAFAVSDGAGETVAESSRSETAARATIFARVVSLMNGRSGLRPEVLTLLCDMLNNGVTPLL--PKDGGEGPSLARAVLGAGSCLFKGEVRPLLEVLSSCELSPLSTLTGREGSTLELGRFDQIGTAAVAAAAASNAIEIADGVAALTCEATRANTAPFDALNFDVRPHRGQIACADRLRLMLDGSKSANSTKGDSTVDPEAISTIPQYHGPARDAIAAAGRSIMTEFNGSDAGPLGVGDGCVQPRDGKQTELTATFLTEAVRVLTSGCRERSQALQG------SANGASSCGGGGDVFGVEGVLRELQSALAEEMALSVAVLRQXXXXXXXXXXXXXXXXXXX-----XXXXXXSGAPGGGVDTAGMSAAQIAKIEAKRXXXXXXXXXXXXXXXXXXKAAGLGVGCAELKQRVAPAGAAAAVGADEIAAKIDPFRTGEQAMGPFLCAMAERLSNGGAQRKPKIAKGARDFLPEQMRVREQAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRSICSAIDKLDKMDWAEVRQEMVEEKGLAPDSADKIGGFVNGNAGGAKELWAKLTEEKKFGDHPDAVAAMSELELLFKYLDAMGTLQYVSFDLSLARGLTYYTGLIYECVVVDGGIRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQEERGDKGAPSNVQVRSSSV 858          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A6H5KM67_9PHAE (Histidine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KM67_9PHAE)

HSP 1 Score: 993 bits (2567), Expect = 0.000e+0
Identity = 532/686 (77.55%), Postives = 584/686 (85.13%), Query Frame = 1

Query: 1129 MTEFNGSDAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSIGMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLA-GVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLPIGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFSGS-EVPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGCTIVTHEPRL 3180
            MTEFNGSDAGPLG G GCV+PRD KQT L V +LSEAVR+LTSG R+R   L+G     S+  G    G  + ++GVLRELQ ALAEE++LS+ +LR E                    QPPQ     + A  G  DT+G + AQI KIE KR  XXXXXXXXXXXXXXX KAAGLGVGCAEL+QRV+PAG   A G +EI+  IDPFRTG+QAMGPFL A+AE+LS GGAQRKPKIAKGARDFLPEQMR+REQAF AIR VFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHG+GNIKR+HIAKVYRRDQPNVQLGRFREFYQCDFDIAG+YG MVPDAEVLSVACEILS+LPIG FQIKLNHRRLLDAILDICGCPPEKFR+ICSAIDKLDK++W+EVRQEMVEEKGLAPD+ADKIGGFVNGNAGG KELWAKL E+K FGDHPDA AAMSELELLFKYLDAMGTL+YVSFDLSLARGLTYYTGLIYEC+VVDGG+RVGSIAAGGRYDNLVGMFS S + PCVGVSIGIERVLTIMEKRLQEE+ + G   NVQVYVASVGP LLEERMRVGRLLW+NDISAEYST +NPK +KQMNEVLQRGIPFMVILGE+EV+QGVV++KDM   T+E M RE++P ALL AGCT+VT +  L
Sbjct:    1 MTEFNGSDAGPLGVGDGCVQPRDGKQTELTVTFLSEAVRVLTSGCRERSQALQGSANGASSGGGG---GDVTGVEGVLRELQFALAEEMALSVAVLRQEXXXXXXXXXXXXXXXXXXXIQPPQ-----SGAPGGGVDTAGMSAAQIDKIEAKRAAXXXXXXXXXXXXXXXXKAAGLGVGCAELKQRVAPAGAAAAVGADEIAAKIDPFRTGEQAMGPFLCAMAERLSNGGAQRKPKIAKGARDFLPEQMRVREQAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSALPIGNFQIKLNHRRLLDAILDICGCPPEKFRSICSAIDKLDKMDWAEVRQEMVEEKGLAPDSADKIGGFVNGNAGGAKELWAKLTEEKKFGDHPDAVAAMSELELLFKYLDAMGTLQYVSFDLSLARGLTYYTGLIYECVVVDGGIRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQEERGDKGAPSNVQVYVASVGPGLLEERMRVGRLLWSNDISAEYSTLDNPKFSKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDVPQALLAAGCTLVTQQAGL 678          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A835Z9D3_9STRA (AA_TRNA_LIGASE_II domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9D3_9STRA)

HSP 1 Score: 744 bits (1920), Expect = 3.080e-249
Identity = 462/856 (53.97%), Postives = 559/856 (65.30%), Query Frame = 1

Query:  328 VLVGARKVLPSDVLLVAVHGARIDLNQAALEKACPRT----SQASSEGDASYDASVGDAEFSPDCAMSETATRAALFARIVSLMNGRSGLRPDVVRFLADLLNNGIIPLLLAQREGGEGAAVAKAVLGAGLCMFKGQIRPLSEVASECEISPLEALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKSGEV-DAKAISCIPQYHGPARDAISAVCRSIMTEFNGSDAGPLGEGKGCVRPRDDKQTSL-AVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSIGMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAG-------LGVGCAELRQRVSPA----GEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLPIGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSE------VRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFSGSEVPCVGVSIGIERVLTIME 2823
            V VGARK+L +DV+ VA   A++DL+ A L++         S+AS    A++DA+   A    +  + E   RAA+ AR+ SLM  RS +R + V  LA LLN G+ PLL A  EG EGAAVA+A+LG G       I+PL+   +   ++PL+ LT  EG TL L  F   G   +  AAA  A+++ADG AA++ +   A + P +  ++D  R HRGQME AER+RL+L GS+S NS  +    D  A+  +PQYHGPAR+A+ A  +   TE N ++AGPL        P   +Q S  + + L+ A+ +L  G R RLA +    G G    GA    +   L G L  L+ ALAEEL+ +    R                                     A   +G T  QIAK   K       XXXXXXXXXXX            LG G AE+++RV  A    GEP A  E +   +D    G+  +G  +A L EKL +GGA+RKPK+ KG RD LPEQMR+REQAF AIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLAL+ +GNIKR+HIAKVYRRDQPN+  GR+REFYQCDFDIAG+YGPMV DAEVLS ACEILS LPIG FQ+KLNHRRLLDAILDICGCP EKFRTICSAIDKLDK  W E      VR+EMVEEKGLAP+AADKIGGFV   +G  ++LW +L ++  FG HPDAAAAM EL LLF YLDAM +L+++ FDLSLARGLTYYTGLIYEC+V+DG  +VGSIAAGGRYDNLVGMFS ++ PCVGVSIGIERV  IME
Sbjct:    2 VTVGARKLLNADVMAVATGAAKVDLDAATLDRCGAEAQYLKSKASPGATAAFDAAAL-AGIPEESVLPEAEARAAIVARLSSLMQCRSCIRAEAVSLLASLLNAGLCPLLPAS-EGDEGAAVARALLGMGSAYTPTGIQPLAGAMTTAGLAPLQELTTMEGDTLRLRAFAATGATCMAAAAAXTALDLADGAAALSLDACAAFSEPLQGAHFDVYRQHRGQMEVAERVRLLLTGSRSLNSPVAPVYQDDDALRAVPQYHGPAREAVLAAGKVAETELNSAEAGPLTRRHDSAGPYHPQQASCCSYQPLAHALLILMQGSRARLAAMSAAAGNGI---GAS---TYVPLRGALSSLRRALAEELAAAA--TRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADPDAGLTPEQIAKRREKAEKNAALXXXXXXXXXXXDXXXXXXXXXXLLGDGTAEIKRRVMAAAGGEGEPSA--EAVLSAVDFNCAGEGTLGGVIAGLIEKLGSGGARRKPKLPKGTRDQLPEQMRVREQAFEAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALNSVGNIKRFHIAKVYRRDQPNLARGRYREFYQCDFDIAGAYGPMVADAEVLSAACEILSGLPIGDFQVKLNHRRLLDAILDICGCPAEKFRTICSAIDKLDKAPWEEASDHHRVRKEMVEEKGLAPEAADKIGGFVT-YSGPPRQLWQQLTDEAKFGQHPDAAAAMGELALLFDYLDAMDSLRFIQFDLSLARGLTYYTGLIYECVVLDGETKVGSIAAGGRYDNLVGMFSSTQTPCVGVSIGIERVFAIME 844          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A2D4BVR0_PYTIN (Histidine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BVR0_PYTIN)

HSP 1 Score: 652 bits (1681), Expect = 1.500e-212
Identity = 450/959 (46.92%), Postives = 567/959 (59.12%), Query Frame = 1

Query:  319 ATVVLVGARKVLPSDVLLVAVHGARIDLNQAALEKACPRTSQASSEGDASYDASVGDAEFSPDCAMSETA--TRAALFA---RIVSLMNGRSGLRPDVVRFLADLLNNGIIPLLLAQREGGEGAAVAKAVLGAGLCMF--KGQIRPLSEVASECEISP-LEALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKSGEVDAKAISCIPQYHGPARDAISAVCRSIMTEFNGSDAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALA--EELSLSIGMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLP-IGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFS--GSEVPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGC 3153
            AT V +   K+   DV  VA  GA++  + AA          A   GD      V   E   D A +  A  TRAAL     R+V L    S     + RF+ADLLN+ ++PLL +         V  A    GL      G+I+ LS    +  I+P + AL+ +E   LS      + +  L V+ A   + +AD VAA+TCE   A++ PFEA   DA RPHRG +  A  LRLMLE SK  N+ K    DA  I  IPQYHGPARDA++A  +++  E N +    +    G   P+  K T LA     +A+ +L  G  +R  V+     A +  +       +     ++ E +LA A  ++    +   +AE +  A E ++  K       +  +L A                EAQ  KI  KR  XXXXXXXXXXXXXXX  +  +G G A LRQ V   G   A V       + F         F+  L  +L +GGA+RKPKIAKGA+DFLP QM++RE+ F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKRYHIA+VYRRD P +  GRFREFYQCDFDIAG+Y PM+PDAEVLS+  E+L   P +G F++KL+HR LLDAIL ICG P +KFRT CSAIDKLDK  W+ VR EMV+EKG+    AD+I  FV    G  +EL   + E+K FGD+ DAA AM EL  LF+YLDAMG L +VSFDLSLARGL YYTGLIYE ++      VGSIAAGGRYDNLVGMFS  G ++PCVGVS+GIER+  I++     EK +    P  QV VAS G +LL ER+ V + LW  +ISAE    ENPK  KQ+   L+ GIPFMV++GE+E+ +G VK+KDM ++ E  + R EM   LL   C
Sbjct:    2 ATKVTIAGAKLQLEDVCSVAALGAKVAADVAAQSV----DHDAPMMGD-----EVVAVELPSDAAAAHGALVTRAALAVLLDRVVRLP--ASAATEGLARFIADLLNHNVLPLLPSGSTDATVLVVLAAAQSRGLARVGTDGKIQELSMALMDASIAPPVWALSRRELAALSETPVVLLAVTTLVVSGARGLLPVADAVAALTCEVVQASSLPFEAEYVDASRPHRGIVTSATNLRLMLETSKFVNAPKDAP-DADCIRHIPQYHGPARDAVAAAAKTVELELNSA----VPTTAGWFHPQALK-TVLASTV--DALTVLLQGSNERRRVVAPEASAIAPQSQCAVELVEVTTQALVAEAELAFAFFDKEEQQLKAKQAESNARAQEAAE--KXXXXXXXEDEKLAAM--------------PEAQRLKILEKRRKXXXXXXXXXXXXXXXXDSVRVGAGSAALRQHVQSLGAWAAHV-------NAFDARPSGFSQFVEELFARLGSGGARRKPKIAKGAQDFLPPQMQLREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHNPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDIAGTYAPMLPDAEVLSIGIEVLQQFPELGPFKVKLSHRVLLDAILAICGVPADKFRTTCSAIDKLDKEPWAAVRSEMVDEKGIPEAVADRIQHFVT-RVGSPRELHRAMTEEKLFGDNADAARAMGELGTLFEYLDAMGVLDFVSFDLSLARGLDYYTGLIYEFVLTSPEHNVGSIAAGGRYDNLVGMFSATGQQIPCVGVSMGIERIFGILQAHA--EKTQAINRPTAQVLVASTGSSLLLERLSVCKELWRANISAEIMQIENPKFKKQLQHALETGIPFMVVIGEDELAKGEVKVKDMASKEEVSVARSEMVAELLRRSC 915          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A3M6V9G4_9STRA (Histidine--tRNA ligase n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6V9G4_9STRA)

HSP 1 Score: 640 bits (1652), Expect = 6.400e-210
Identity = 389/816 (47.67%), Postives = 505/816 (61.89%), Query Frame = 1

Query:  718 KGQIRPLSEVASECEISPLEALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKSGEVDAKAISCIPQYHGPARDAISAVCRSIMTEFNGSDAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSIGMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLP-IGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFSGSE--VPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGC 3153
            K QI P    + E  +S    L+  E T ++   F  +    L V  A + + IAD VA +TCET  A+T+ FEA   DA RPHRG +  A+ LR ML+GSK  NS++ G  D  A+ CIPQYHGPARDAI    +++  E N +  G     K        +    A+    EA+ +L  G  +RL +L         D         +    +++    AL  EL  S+  L  EE++A  ++  A K       Q   L A AAAA       +   EAQ  KI  KR                 K    +G+G   +RQ +   G+      + S V+  F     ++  +L  L  +L +GG +RKPKIAKG++DFLP QM +RE+ F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKRYHIA+VYRRD P +  GRFREFYQCDFDIAG+Y PM+PDAEVLS+  E++   P +G  ++KL+HR LLDAIL ICG P +KFRT CSAIDKLDK  WSEVR+EMV+EK +  + AD+I  FV    G  ++L  +L ++K FGD+ +A  AM +L LLF YLDAMG L +VSFDLSLARGL YYTGLI+E ++     +VGSIAAGGRYDNLVGMFS +E  +PCVGVS+GIER+  I+E   +++   T TSP+ QV VAS   NL+  R+ + + LW ++ISAE    ENPK  KQ++  L+RG P+MV++GE+E+ +GVVK+KDM ++ E  +PR E+   LL  GC
Sbjct:    6 KDQISPAQVSSLELPVS----LSRTEQTAINNTPFVLLAETVLAVGGARSLLPIADAVAGITCETLHADTAAFEAEFVDAARPHRGIITSAQNLRSMLDGSKLINSQQEGVTDVAAVCCIPQYHGPARDAILVTYKAVEVEINAT-FGDRAAAKRVGASIHPQALKTALSTTMEALHVLYQGSVNRLQLL---------DIKVTDARDITFAVDLVQATATALVHELVPSLKFL--EEEEAQLKLRMAKK---NANVQEAALKAAAAAAKEDE-KIAAMPEAQQNKILEKRRKKLEKQKEKENAKKCGKDELKIGLGSQAVRQYILALGDGW----QSSVVMSAFDLRASSLSVYLEDLFVRLGSGGTRRKPKIAKGSQDFLPYQMALREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHSPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDIAGTYAPMLPDAEVLSIGIEVMQQFPELGPVKVKLSHRLLLDAILAICGVPADKFRTTCSAIDKLDKEPWSEVRREMVQEKDILEEVADRIEPFVC-KVGSPRDLHTQLVKEKMFGDNANARRAMDDLALLFNYLDAMGVLDFVSFDLSLARGLDYYTGLIFEFVLTSPEHKVGSIAAGGRYDNLVGMFSATEQQIPCVGVSLGIERIFGILEAHAKKKSANT-TSPS-QVLVASTSNNLIMPRLELCKQLWLSNISAEVVQTENPKFIKQLHYALERGTPYMVVIGEDELTKGVVKVKDMASKDEVTVPRSELVAELLRRGC 794          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A3R7KTR4_9STRA (Histidine--tRNA ligase n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7KTR4_9STRA)

HSP 1 Score: 644 bits (1662), Expect = 1.460e-209
Identity = 398/806 (49.38%), Postives = 507/806 (62.90%), Query Frame = 1

Query:  778 ALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYD-ARPHRGQMECAERLRLMLEGSKSCNSKKSGEVDAKAISCIPQYHGPARDAISAVCRSIMTEFNGS--DAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSIGMLRAEE--------DKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLP-IGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFSGSE--VPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGC 3153
            +L+  +  TL+   F  +  A L V  A   + IAD VAAVTCET  A+++ FEA   D ARPHRG +  A+ LRLML+GSK  NS K G  +  A+ CIPQYHGPARDA+    +++  E NG+  D+         + P+  K    A+   +EAV +L  G  +RL VL         D+GA    + + +  ++     AL+ EL  S      EE        +K A               +  +L A                EAQ  KI  KR  XXXXXXXXXXXXX  K    +G+G   LRQ +    + L G  +       F     ++  FL  L  +L +GGA+RKPKIAKG++DFLP QM +RE+ F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKRYHIA+VYRRD P +  GRFREFYQCDFD+AG+Y PM+PDAEVLS+  E+L   P +G  ++KL+HR LLDAIL ICG P +KFRT CSAIDKLDK  W++VR+EMVEEK +  + AD+I  FV    G  +EL A+L ++  FGD+ +A  AM +L LLF YL AMG L ++SFDLSLARGL YYTGLIYE ++      VGSIAAGGRYDNLVGMFS ++  +PCVGVS+GIER+  I++     EK+    +P  QV VAS G  LL  R+ + + LW ++ISAE    ENPK  KQ++  L+RG PFMV++GE+E+ +GVVK+KDM ++ E  +PR E+ N LL  GC
Sbjct:  156 SLSPADQVTLTSVPFQLLAEAVLAVGGACALLPIADAVAAVTCETLRADSAAFEAEFVDVARPHRGIVTSAQNLRLMLDGSKFVNSLKEGATEVAAVRCIPQYHGPARDAVLVAYKTVEAEINGATADSAVAKRAGAGIHPQALKT---ALSATTEAVHVLLQGSVNRLHVL---------DSGAPDVPNTTSVIELVEATAAALSRELEPSFKFFEDEEAQLKLRLAEKNAKAQEAXXXXXXXXXXEDEKLAAM--------------PEAQRNKILEKRRKXXXXXXXXXXXXXSSKDELKIGLGSLALRQHL----QALGGAWQNCVQSAAFDLRASSLSQFLDELFVRLGSGGARRKPKIAKGSQDFLPHQMALREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHSPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDVAGTYAPMLPDAEVLSIGIEVLQQFPELGPVKVKLSHRLLLDAILAICGVPADKFRTTCSAIDKLDKEPWADVRREMVEEKDIPAEVADRIEPFVC-KVGSPRELHAQLMKESIFGDNANARRAMDDLALLFDYLGAMGVLDFISFDLSLARGLDYYTGLIYEFVLTSPEHHVGSIAAGGRYDNLVGMFSATDQQIPCVGVSLGIERIFGILQAHA--EKQTANVTPPSQVLVASTGSALLLPRLELCKQLWVSNISAEVMQTENPKFIKQLHYALERGTPFMVVIGEDELAKGVVKVKDMLSKDEVTVPRSELVNELLRRGC 928          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A7S2LH49_9STRA (Histidine--tRNA ligase n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2LH49_9STRA)

HSP 1 Score: 642 bits (1657), Expect = 8.020e-209
Identity = 432/959 (45.05%), Postives = 574/959 (59.85%), Query Frame = 1

Query:  322 TVVLVGARKVLPSDVLLVAVHGARIDLNQAALEKACPRTSQASSEGDASYDASVGDAEFSPDCAMSETATRAALFARIVSLMNGRSGLRPDVVRFLADLLNNGIIPLLLAQREGGEGAAVAKAVLGAGLCMFKGQIRPLSEVASECEISPLEALTIQEGTTLS-LGRFDRIGMAALGVAAATNAIEIADGVAAVTCET--TCANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKSGEVDAKAISC-----IPQYHGPARDAISAVCRSIMTEFNGSDAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSI-----GMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLPIGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMF--SGSEVPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAG 3150
            T+++  AR++   ++  +A   A++++ Q   ++      Q+ ++  ++      D   S D  +S  AT A+L    +++  GR  +R D     A  L++ I+ ++    EGG G  +       G               S  E+   E   ++ G  L  L    RI +  L  A   ++  I+D +A+++ E   +  +   F + NYD  RPHRG +E A  +R  L+GS +  + +     +   SC      PQYHGPAR++I+  C+++  E N S+      G   +   DD    LA K + E+V  L++G   R         A S      +      L+  +  LQ +L  E  +        + + E +  A E  +A +   RG    P      A+   G  + +G +EAQ AKI  KR    XXXXXXXXXXXXX        G A L    S  G  +A +  +         GD+A+   L    E L +GG QRKPK+AKG RD+LPEQM IR++AF  IRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR+LA++ +GNIKR+HI KVYRRDQP +  GR+REFYQCDFDIAG YG MVPD+E L+VACEIL SLPIG F IKLNHRRLLDAILD+CG P +KFRTICSA+DKLDK  WSEVR+EMVEEKGL+ D ADKIG FV    G   +L+  L E K+FG+H  A+ AM +L +LF+YL+AMG LK++SFDLSLARGL YYTG+IYE + ++G  +VGSI  GGRYD LV MF  +G   PCVGVS+GIERV T+ME+RL++E+  +   PNV V VAS G N+L ERM++  +LW  +ISAE+S  ENPKL  ++   L R IPFMVI+GEEE KQG  K+KD+N RTE+ +   ++   L + G
Sbjct:    4 TILIGKARRITLDELASIATGSAKLEMQQQQTDEEAAEQPQSMADVASALQKLSLDTSSSGDL-LSAEATIASLTLLALTISQGRI-IRGDC----AAKLSSAIVDIVNNVLEGGSGEPLRLPSNADGF------------TTSVNELVGGELAILENGPYLERLISIARISLC-LNKARTLSSKSISDPIASLSIERLGSALSIDSFSSTNYDELRPHRGCIESASVIRACLQGSTNVAANEKNVTPSSDDSCKYAKLTPQYHGPARESITGACKTMELEMNCSEM----NGSASL---DDTVAMLAAKSVLESVLTLSTGSSMRCGSKTDTTQALSAMCNLAEVAPS--LETAVVTLQNSLESEAKMGCVFVDDELAKKEAELKAKEEEKAKRTAARGTNNNPN-----ASGGDGKDEFAGMSEAQKAKILKKRAEKEXXXXXXXXXXXXXXAG-----GAAAL---TSIFGAGIAAIYPLLSTKSDL--GDEALVSELEQAIEALLSGGMQRKPKVAKGTRDYLPEQMAIRDKAFTIIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFLAVNAVGNIKRFHIGKVYRRDQPQLSKGRYREFYQCDFDIAGVYGRMVPDSECLAVACEILDSLPIGDFGIKLNHRRLLDAILDLCGVPADKFRTICSAVDKLDKEPWSEVRREMVEEKGLSGDVADKIGEFVV-LKGKPWDLYNSLMESKSFGNHKGASEAMEDLRILFEYLEAMGKLKFISFDLSLARGLDYYTGVIYEAVCMNGNTQVGSIGGGGRYDTLVSMFQEAGKVTPCVGVSVGIERVFTLMEERLRQEQGGSIKQPNVTVLVASAGDNMLRERMKLTNVLWDANISAEFSQQENPKLKFEIANALDRQIPFMVIVGEEETKQGKCKVKDLNARTEDTVDVSDLVKTLRSKG 918          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A662YHR8_9STRA (Histidine--tRNA ligase n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662YHR8_9STRA)

HSP 1 Score: 641 bits (1653), Expect = 2.690e-208
Identity = 437/968 (45.14%), Postives = 564/968 (58.26%), Query Frame = 1

Query:  316 AATVVLVGARKVLPSDVLLVAVHGARIDLNQAALEKACPRTSQASSEGDASYDASVGDAEFSPDCAMSET--ATRAALFARIVSLMNGRSGLRPDV-VRFLADLLNNG-IIPLLLAQREGGEGAAVAKAVLGAGLCMFKGQIRPLSEVASECEISPLEALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKSGE---VDAKAISCIPQYHGPARDAISAVCRSIMTEFNGS--DAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSIGMLRAEE--------DKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPL-AGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLP-IGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFSGSE--VPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGC 3153
            AA  V +GA K+  +DV  VA  GA++     A+E   P    AS+   A   A   D   S D A S     TRA L   +  L+  R+         F+A LLN+  + PL+           +  A  G G      +  P+ +V     ++  + L+  E T ++      +  A L V  A   + +AD VAAV+CET  A+++ FEA   DA RPHRG +  A+ LRLML+GSK  NS+  G     D  A+SCIPQYHGPARDA+    +++  E NG+  D+         V P+  K   +A+   ++A+ +L  G  DRL VL         D+ A      +D   ++     AL +EL  S+     EE        +K A               +  +L A                EAQ  KI  KR    XXXXXXXXXXXX K    +G+G   LRQ +   G    A V+  +     F      +  FL  L  +L +GGA+RKPKIAKG++DFLP QM +RE+ F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKRYHIA+VYRRD P +  GRFREFYQCDFDIAGSY PM+PDAEVLS+  E+L   P +G  ++KL+HR LLDAIL ICG P +KFRT CSAIDKLDK  WS VR+EMVEEK +    AD+I  FV    G  +EL A+L ++  FGD+ +A  AM++L LLF YLDAMG L ++SFDLSLARGL YYTGLIYE ++      VGSIAAGGRYDNLVGMFS ++  +PCVGVS+GIER+  I++     +K+    +P  QV VAS G  LL  R+ + + LW  +ISAE    ENPK  KQ++  L+RG P+MV++GE+E+ + VVK+KDM ++ E  + R E+   LL  GC
Sbjct:    2 AAVRVTLGAGKLSIADVCCVAALGAQV-----AVEAGSPHGGSASAAAPA---APTADVTLSDDEASSYALPTTRAMLTLLLHRLLRLRASAATQANAAFVATLLNHAELTPLVPMSAADSAVLVLLAATEGRGFV----RSGPMGQVLQRASVALPDPLSAAEKTAVTSVPLALLAEATLAVGGARALLPVADAVAAVSCETLRASSAAFEADFVDAARPHRGIVTSAQNLRLMLDGSKFVNSQTEGSNATTDMAAVSCIPQYHGPARDAVLVAYKTVEAEINGAVGDSAAAKRAGAGVHPQAVK---MALAATTQALGVLLQGSVDRLQVL---------DSKAADAPKTTDAVELVEATAAALVQELVPSLAFFDEEEAQLKLRVAEKNAKAQEAXXXXXXXXXXEDEKLAAM--------------PEAQRNKILEKRRKKLXXXXXXXXXXXXAKDELKIGLGSLALRQHLVGLGASWQASVQGAA-----FDLRVSGLSLFLEELFVRLGSGGARRKPKIAKGSQDFLPHQMALREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHSPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDIAGSYAPMLPDAEVLSIGIEVLQQFPELGPVKVKLSHRLLLDAILAICGVPADKFRTTCSAIDKLDKEPWSNVRREMVEEKDIPETVADRIEPFVC-KVGSPRELHAQLVKEGLFGDNANARHAMNDLALLFDYLDAMGVLDFISFDLSLARGLDYYTGLIYEFVLTSPEHHVGSIAAGGRYDNLVGMFSATDQQIPCVGVSLGIERIFGILQAHA--DKQAASAAPPSQVLVASTGGALLLPRLELCKQLWNANISAEVMQTENPKFTKQLHYALERGTPYMVVIGEDELTKDVVKVKDMASKDEVTVARSELVAELLRRGC 923          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: K0RV05_THAOC (Histidine--tRNA ligase (Fragment) n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RV05_THAOC)

HSP 1 Score: 630 bits (1625), Expect = 1.230e-205
Identity = 400/815 (49.08%), Postives = 501/815 (61.47%), Query Frame = 1

Query:  829 IGMAALGV----AAATNAIEIADGVAAVTCETT--CANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKS-GEVDAKAISCI------PQYHGPARDAISAVCRSIMTEFNGSDAGPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDL-------DGVLRELQLALAEELSLSIGMLRAEEDKAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADT-------SGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSAGGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLPIGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWA---KLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMF--SGSEVPCVGVSIGIERVLTIMEKRLQEEKEETGTSPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGCTIVTHEP 3174
            + +A LGV    A + +A  + D ++A++ E          F   NYD  RPHRG +E A  LR  L GS    S K+  + +    +C+      PQYHGPAR+AI++ C+++  E N S+ G   E        DD    LA       +  L +G   R+             +  C  GS S L       +  + +L+ AL  E  L    + +E  K   E+            +  +   TA  A SG  +        +G TEAQ AKI  KR     XXXXXXXXXXXX  AAG     + +    +    PL   +++          + A+   L    EKL +GG QRKPK+A G RD+LPEQM IR+QAF+ IRRVF RHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGE+LALRYDLTVPFAR+LA++ +GNIKR+HI KVYRRD+P +  GR+REFYQCDFDIAG YG MVPD+E L+VACEIL +LPIG F IKLNHRRLLDAILDICG P +KFRTICSA+DKLDK  WSEVR+EMVE+KGL  D AD+IG FV        E WA    L E   FGDH  AA AM +L + F YL+AM  LK++SFDLSLARGL YYTG+IYE + ++G  +VGSI  GGRYDNLV MF  +G   PCVGVS+GIERV T+ME+RL++E+  +   PNV+V VAS G NLL ERMR+ R+LW  +ISAE+S  ENPKL  ++   L R IPFMVI GEEE ++G  K+KD+ NRTEE +   ++   L   G   +  +P
Sbjct:   48 VSLARLGVLLAQARSLSAKSVCDPISALSAERLGQYLTLDAFSTTNYDELRPHRGCVESASVLRTCLAGSTMVASSKTLKQPEGSDATCVKYAKSAPQYHGPAREAIASACKTMELELNCSEGGDAAE-------LDDSIALLACASALGGITTLVNGSSSRIG-----------SDSKCDAGSPSTLSTMANALESAVEQLKAALESEAKLGCDYVVSELSKREAELKA---------KEAEKAKRTAERAGSGNPNQKGDKDEFAGMTEAQKAKILKKRAEKEAXXXXXXXXXXXX--AAGGEAALSSIFGTGTAFLYPLLSGKDLD-------LSNSALLESLEGAIEKLQSGGMQRKPKVALGTRDYLPEQMAIRQQAFSIIRRVFLRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGEMLALRYDLTVPFARFLAVNSVGNIKRFHIGKVYRRDRPVMSKGRYREFYQCDFDIAGHYGRMVPDSECLAVACEILDALPIGDFGIKLNHRRLLDAILDICGVPADKFRTICSAVDKLDKEPWSEVRREMVEDKGLTVDVADRIGEFVVLKG----EPWAMYNSLIEGGKFGDHKGAADAMEDLRICFTYLEAMDKLKFISFDLSLARGLDYYTGVIYEAVCMNGNTQVGSIGGGGRYDNLVSMFQEAGKVTPCVGVSVGIERVFTLMEERLRKEQGGSIKRPNVKVLVASAGENLLHERMRLARVLWDANISAEFSQQENPKLKFEIANALDRQIPFMVISGEEEAREGKCKVKDLANRTEETVAVSDLVTILCGKGVAPLILQP 822          

BLAST of mRNA_A-nodosum_M_contig104.85.5 vs. uniprot
Match: A0A5D6Z195_9STRA (Histidine--tRNA ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Z195_9STRA)

HSP 1 Score: 634 bits (1636), Expect = 1.670e-205
Identity = 415/906 (45.81%), Postives = 535/906 (59.05%), Query Frame = 1

Query:  517 SETATRAALFARIVSLMNGR-SGLRPDVVRFLADLLNNGIIPLLLAQREGGEGAAVAKAVLGAGLCMFKGQIRPLSEVASECEIS------PLEALTIQEGTTLSLGRFDRIGMAALGVAAATNAIEIADGVAAVTCETTCANTSPFEAPNYDA-RPHRGQMECAERLRLMLEGSKSCNSKKSGEVDAKAISCIPQYHGPARDAISAVCRSIMTEFNGSDA-GPLGEGKGCVRPRDDKQTSLAVKYLSEAVRMLTSGVRDRLAVLRGMGGAGSTDNGACKPGSDSDLDGVLRELQLALAEELSLSIGMLRAEED--------KAAGEVSQAPKFDGRGPTQPPQLPATAAAASSGAADTSGKTEAQIAKIEVKRXXXXXXXXXXXXXXXXXKKAAGLGVGCAELRQRVSPAGEPLAGVEEISRVIDPFRTGDQAMGPFLAALAEKLSA----GGAQRKPKIAKGARDFLPEQMRIREQAFAAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGLGNIKRYHIAKVYRRDQPNVQLGRFREFYQCDFDIAGSYGPMVPDAEVLSVACEILSSLP-IGTFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKLEWSEVRQEMVEEKGLAPDAADKIGGFVNGNAGGGKELWAKLDEQKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECIVVDGGVRVGSIAAGGRYDNLVGMFS--GSEVPCVGVSIGIERVLTIMEKRLQEEKEETGT---SPNVQVYVASVGPNLLEERMRVGRLLWANDISAEYSTAENPKLNKQMNEVLQRGIPFMVILGEEEVKQGVVKMKDMNNRTEEVMPREEMPNALLTAGC 3153
            S   +RA L   +  L+  R S    +  +F+  LLN    PL  A       AA+  A+ G GL   +G       VA    +       P   L+  E   +       + ++ L VA A   + ++D VAA+TCET  AN + FEA   DA RPHRG +  A  LRL+LE SK  NS+K    D   +  IPQYHGPARDA+ A  ++I  E N +   G      G + P+  K T+L      + VR+L +G  +R AVL               P S   ++ ++     +LA+ELSLS+    AE++        K+A               +  +L A                EAQ  K+  KR  XXXXXXXX       +    +GVG A  R  V+P G  L     ++  ++PF   D   G   A + E+L A    GGA+RKPKIAKGA+D LP QM++RE+ F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKRYHIA+VYRRD P +  GRFREFYQCDFD+AGSY PM+PDAEVL+VA E+L   P +G F++KL+HR LLDA+L ICG P +KFR  CSAIDKLDK  W+ VR+E+V++KGL    AD+I  FV    G  + L A L+ ++ FG + DAA AM EL +LF YLDAMG L++VSFDLSLARGL YYTGLIYE ++      VGSIAAGGRYDNLVGMFS  G ++PCVGVS+GIER+  I+    ++ ++ TG    SP+ QV VAS G  L   R+ + + LW  +I+AE    ENPK  KQ++  L+RGIP+MV++GEEE+  G VK+K++  + E  +PR EM   LL   C
Sbjct:   71 SPAVSRAVLVVLLHRLLQLRASAATVESAQFVTALLNADTTPLFAAGPVDSAAAALLAAIEGIGLAR-QGVAAHTETVAVAAALQRAGIALPTTPLSAAEKELMLRAPATLLAVSTLAVAGARGLLPLSDAVAALTCETLRANAASFEAEYVDASRPHRGVVTAATNLRLLLENSKLVNSRKDATADPACVRYIPQYHGPARDAVLAAYKTIELELNSAAVEGAAARSVGALHPQVLK-TALGATL--DGVRVLLTGSNERRAVL-------DASAAPIAPASACAVE-LVEAATASLAQELSLSLRAFDAEDEQLRARLAEKSAKXXXXXXXXXXXXXXEDEKLAAM--------------PEAQRLKVLEKRKKXXXXXXXXDGAKKGGRDEVKIGVGSAAFRAFVAPHGG-LVSAAALAAALNPF---DLRAGTGAALVVEELLARIGSGGARRKPKIAKGAQDLLPHQMQLREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHNPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDVAGSYAPMLPDAEVLAVAVEVLQQFPELGPFKVKLSHRLLLDAVLAICGVPADKFRATCSAIDKLDKEPWAAVRRELVDDKGLPAAVADEIERFVC-RVGAPRALHAALEAEQLFGANADAARAMGELRVLFDYLDAMGVLEHVSFDLSLARGLDYYTGLIYEFVLTSPAHNVGSIAAGGRYDNLVGMFSATGQQIPCVGVSMGIERIFGILLAHAEKVEKATGAVSPSPS-QVLVASTGGALTLARLAICQELWRANIAAEVLQVENPKFTKQLHYALERGIPYMVVVGEEELASGQVKIKEIAAKDEVAVPRGEMVAELLRRRC 944