mRNA_S-firma_F_contig10782.1044.1 (mRNA) Sphaerotrichia firma ET2_F female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_F_contig10782.1044.1
Unique NamemRNA_S-firma_F_contig10782.1044.1
TypemRNA
OrganismSphaerotrichia firma ET2_F female (Sphaerotrichia firma ET2_F female)
Homology
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: D7FYF9_ECTSI (EsV-1-8 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYF9_ECTSI)

HSP 1 Score: 105 bits (262), Expect = 5.490e-27
Identity = 56/111 (50.45%), Postives = 74/111 (66.67%), Query Frame = 1 
Query:    1 GLTPLHGAVILANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSLRRLLTNAPADRAWRRRGLLVL 333
            GLTPLH A  + +T P ++ALL+ GAN +A+D  G   L + C   A     V + DLLLR  ADET  DN+G  A DL++SGA+P+  L+RLL N PADRAWRRR ++V+
Sbjct:   10 GLTPLHVAAYIDDTCPNMLALLQKGANANARDDDGRGSLLLVCNTKASS---VAMADLLLRWGADETAADNDGNTAADLVKSGAEPNKGLQRLLANVPADRAWRRRSMVVM 117
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: D8LCR2_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCR2_ECTSI)

HSP 1 Score: 92.0 bits (227), Expect = 1.000e-19
Identity = 51/111 (45.95%), Postives = 67/111 (60.36%), Query Frame = 1 
Query:    1 GLTPLHGAVILANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSLRRLLTNAPADRAWRRRGLLVL 333
            G+TPLH A    N   +LV L +  AN +A+D  G +PLH+ CM   +        D+LLRS ADET TD +     D++ S    +G L++LL NAPADRAWRRRG LV+
Sbjct:  240 GMTPLHEAAYQGNYPGMLVLLQKGRANANAQDEHGHSPLHILCM-NPQVCSAAACSDILLRSGADETATDEDRHTPADIMASSEGHNGWLQQLLANAPADRAWRRRGTLVM 349
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: D7FYG4_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYG4_ECTSI)

HSP 1 Score: 92.4 bits (228), Expect = 1.300e-19
Identity = 53/111 (47.75%), Postives = 67/111 (60.36%), Query Frame = 1 
Query:    1 GLTPLHGAVILANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSLRRLLTNAPADRAWRRRGLLVL 333
            G T LH A     T   ++ALL+ GAN +A D  G  PLH+ C     R   V V DLLLR  A+ET TDN+G    D I+S  +  G L+RLL NAPA RAWRRRG++++
Sbjct:  326 GSTSLHVAAYW-ETLDTMLALLQKGANANAPDDDGHGPLHLVCKYPKAR--SVAVADLLLRWGAEETATDNDGNTPADFIQSSDESDGRLQRLLANAPAGRAWRRRGMVIM 433
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: A0A6H5JT17_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JT17_9PHAE)

HSP 1 Score: 89.4 bits (220), Expect = 1.140e-18
Identity = 53/111 (47.75%), Postives = 69/111 (62.16%), Query Frame = 1 
Query:    1 GLTPLHGAVILANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSLRRLLTNAPADRAWRRRGLLVL 333
            GLTPLH A     +   + ALLR+GAN+DA+   GLTPLH+ C  T+     V+  DLLL  DADET TD++GR    L+   +     +R+ L NAPADR WR RG+LV+
Sbjct:  238 GLTPLHVAAYYHRSLS-MAALLRSGANVDAEADNGLTPLHMVCK-TSLAPGSVKANDLLLSWDADETTTDHDGRTPKALVSGRSSLRWRVRQKLANAPADRLWRSRGMLVM 346
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: D8LKW6_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKW6_ECTSI)

HSP 1 Score: 88.6 bits (218), Expect = 2.950e-18
Identity = 52/111 (46.85%), Postives = 67/111 (60.36%), Query Frame = 1 
Query:    1 GLTPLHGAVILANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSLRRLLTNAPADRAWRRRGLLVL 333
            GLTPLH A    + +  +  LLR GA+++A+   GLTPLH+ CM    RL   +  DLLLR  ADET  DNEG      I   ++ S +LR +L  AP DRAWRRRG L++
Sbjct:  326 GLTPLHVAAY--HQKASMAELLRKGADVNAESDNGLTPLHMLCM--KPRLNPDDTADLLLRWGADETAIDNEGYTPKARIVGSSNGSQALRHMLDKAPVDRAWRRRGTLIM 432
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: A0A6H5KNU3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNU3_9PHAE)

HSP 1 Score: 76.3 bits (186), Expect = 6.530e-14
Identity = 50/102 (49.02%), Postives = 64/102 (62.75%), Query Frame = 1 
Query:   55 VALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDL----IESGADPSGS-----LRRLLTNAPADRAWRRRGLLVL 333
            +AL ++GANI+A+D +G TPL +A    A R     +VD+LLR  ADET  + +GR A ++    IE  A P        +R LL NAPADRAWRRRGLLVL
Sbjct:  238 LALFKHGANIEARDMRGETPLIIAAR-QAGRQGVTAIVDMLLRCGADETALNPDGRTAAEVVGHHIEYAACPLNKQDLERVRDLLENAPADRAWRRRGLLVL 338
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: A0A6H5JHY9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHY9_9PHAE)

HSP 1 Score: 75.5 bits (184), Expect = 1.300e-13
Identity = 52/121 (42.98%), Postives = 66/121 (54.55%), Query Frame = 1 
Query:    1 GLTPLHGAVILANTRPIL---VALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSL-------RRLLTNAPADRAWRRRGLLVL 333
            G TPLH    +A+    L   +ALLR+GA++  K+    TPLH+     A      E+VDLLLR  ADET     G  A D++    + S SL       R+LL  APADRAWRRRG L+L
Sbjct:  589 GSTPLH----VASQEGRLAAGIALLRHGASVGKKNAAAQTPLHLVA-AKAGSPGTAEMVDLLLRRGADETAVSENGHTAADMVVLKTEESDSLAEDVDRVRKLLARAPADRAWRRRGFLIL 704
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: D8LTL5_ECTSI (MHC_I C-terminus family protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTL5_ECTSI)

HSP 1 Score: 73.2 bits (178), Expect = 6.720e-13
Identity = 56/123 (45.53%), Postives = 70/123 (56.91%), Query Frame = 1 
Query:    7 TPLHGAVI--LANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGS------------LRRLLTNAPADRAWRRRGLLVL 333
            TPLH AV   +A+   I  ALL  GA+I   D    TPLH AC  ++     V  V+LLLR  ADE +T+ EG A  D++  GA PS +            +RR+L  APADR+WRRRGLLVL
Sbjct:  188 TPLHVAVDRRVASIGTI-GALLEGGADIHVFDEIDDTPLHRACKRSS-----VTGVELLLRRGADEELTNEEGDAPADIV--GASPSNATVDEEIEVDNQRIRRMLARAPADRSWRRRGLLVL 302
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: A0A6H5L7V4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L7V4_9PHAE)

HSP 1 Score: 68.9 bits (167), Expect = 2.500e-12
Identity = 44/105 (41.90%), Postives = 60/105 (57.14%), Query Frame = 1 
Query:   52 LVALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDL-IESGADPSG----------SLRRLLTNAPADRAWRRRGLLVL 333
            ++ LL+ GA++  +D+ G T LH+AC       +  E VDLLLR  ADETV D+EG         +GAD +            L +LLT+AP DR+WRRRG LV+
Sbjct:    1 MLTLLQLGADVHVRDSTGATSLHMACYD-----VEPEAVDLLLRWGADETVVDDEGHTPYSYHFPAGADEAEWDEENRRTYKRLSKLLTHAPQDRSWRRRGFLVM 100
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BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Match: D7G6X8_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6X8_ECTSI)

HSP 1 Score: 71.6 bits (174), Expect = 2.700e-12
Identity = 47/102 (46.08%), Postives = 62/102 (60.78%), Query Frame = 1 
Query:   55 VALLRNGANIDAKDTQGLTPLHVACMVTAERLLRVEVVDLLLRSDADETVTDNEGRAAVDL----IESGADPSGS-----LRRLLTNAPADRAWRRRGLLVL 333
            +AL ++G+NI+A+D +G TPL +A    A R     +VD+LLR  ADET  + +GR A ++    IE  A P        +R LL NAP DRAWRRRG LVL
Sbjct:  189 LALYKHGSNIEARDIRGETPLIIAAR-QAGRQGVTAIVDMLLRCGADETALNPDGRTAAEVVGHHIEYAACPLNKQDVERVRDLLENAPVDRAWRRRGFLVL 289
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_F_contig10782.1044.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FYF9_ECTSI5.490e-2750.45EsV-1-8 n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
D8LCR2_ECTSI1.000e-1945.95Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
D7FYG4_ECTSI1.300e-1947.75Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
A0A6H5JT17_9PHAE1.140e-1847.75Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LKW6_ECTSI2.950e-1846.85Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
A0A6H5KNU3_9PHAE6.530e-1449.02Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JHY9_9PHAE1.300e-1342.98Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LTL5_ECTSI6.720e-1345.53MHC_I C-terminus family protein n=1 Tax=Ectocarpus... [more]
A0A6H5L7V4_9PHAE2.500e-1241.90Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G6X8_ECTSI2.700e-1246.08Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_F_contig10782contigS-firma_F_contig10782:778..1110 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma ET2_F female2021-02-24
Properties
Property NameValue
Seed ortholog2880.D7FYG4
PFAMsAnk,Ank_2,Ank_3,Ank_4,Ank_5,Motile_Sperm
Max annot lvl2759|Eukaryota
KEGG koko:K21440
GOsGO:0001508,GO:0002027,GO:0002262,GO:0002376,GO:0002520,GO:0003008,GO:0003012,GO:0003013,GO:0003015,GO:0003205,GO:0003230,GO:0003279,GO:0003283,GO:0003674,GO:0005198,GO:0005200,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005783,GO:0005829,GO:0005856,GO:0005886,GO:0005911,GO:0005938,GO:0006725,GO:0006778,GO:0006779,GO:0006807,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006873,GO:0006874,GO:0006875,GO:0006887,GO:0006888,GO:0006928,GO:0006936,GO:0006937,GO:0006941,GO:0006942,GO:0006996,GO:0007009,GO:0007010,GO:0007154,GO:0007163,GO:0007165,GO:0007267,GO:0007275,GO:0007507,GO:0008015,GO:0008016,GO:0008092,GO:0008093,GO:0008104,GO:0008150,GO:0008152,GO:0009058,GO:0009893,GO:0009987,GO:0010256,GO:0010468,GO:0010522,GO:0010604,GO:0010628,GO:0010638,GO:0010646,GO:0010880,GO:0010881,GO:0010882,GO:0010959,GO:0012505,GO:0014704,GO:0014731,GO:0015672,GO:0016020,GO:0016043,GO:0016192,GO:0016323,GO:0016528,GO:0016529,GO:0018130,GO:0019222,GO:0019438,GO:0019722,GO:0019725,GO:0019899,GO:0019900,GO:0019901,GO:0019932,GO:0022898,GO:0023051,GO:0023052,GO:0030001,GO:0030003,GO:0030011,GO:0030016,GO:0030017,GO:0030018,GO:0030029,GO:0030048,GO:0030054,GO:0030097,GO:0030099,GO:0030154,GO:0030218,GO:0030315,GO:0030424,GO:0030507,GO:0030673,GO:0030674,GO:0030863,GO:0031252,GO:0031253,GO:0031256,GO:0031430,GO:0031647,GO:0031672,GO:0031674,GO:0032409,GO:0032411,GO:0032412,GO:0032414,GO:0032501,GO:0032502,GO:0032589,GO:0032879,GO:0032940,GO:0032970,GO:0033013,GO:0033014,GO:0033036,GO:0033043,GO:0033267,GO:0033292,GO:0033365,GO:0034101,GO:0034394,GO:0034613,GO:0034641,GO:0034762,GO:0034764,GO:0034765,GO:0034767,GO:0035088,GO:0035090,GO:0035556,GO:0035637,GO:0036309,GO:0036371,GO:0042383,GO:0042391,GO:0042592,GO:0042692,GO:0042995,GO:0043005,GO:0043034,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043266,GO:0043268,GO:0043269,GO:0043270,GO:0043292,GO:0044057,GO:0044093,GO:0044237,GO:0044249,GO:0044271,GO:0044291,GO:0044304,GO:0044325,GO:0044422,GO:0044424,GO:0044425,GO:0044444,GO:0044448,GO:0044449,GO:0044456,GO:0044459,GO:0044463,GO:0044464,GO:0045197,GO:0045199,GO:0045202,GO:0045211,GO:0046483,GO:0046903,GO:0046907,GO:0048193,GO:0048468,GO:0048513,GO:0048518,GO:0048522,GO:0048534,GO:0048731,GO:0048821,GO:0048856,GO:0048869,GO:0048872,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050821,GO:0050896,GO:0051049,GO:0051050,GO:0051117,GO:0051128,GO:0051130,GO:0051179,GO:0051186,GO:0051188,GO:0051234,GO:0051239,GO:0051270,GO:0051279,GO:0051282,GO:0051641,GO:0051649,GO:0051716,GO:0051899,GO:0051924,GO:0051928,GO:0055001,GO:0055065,GO:0055072,GO:0055074,GO:0055076,GO:0055080,GO:0055082,GO:0055117,GO:0060047,GO:0060048,GO:0060090,GO:0060255,GO:0060306,GO:0060307,GO:0060341,GO:0061024,GO:0061061,GO:0061245,GO:0061337,GO:0061515,GO:0065007,GO:0065008,GO:0065009,GO:0070252,GO:0070296,GO:0070727,GO:0070838,GO:0070972,GO:0071704,GO:0071840,GO:0071944,GO:0072359,GO:0072503,GO:0072507,GO:0072511,GO:0072657,GO:0072659,GO:0086001,GO:0086002,GO:0086003,GO:0086004,GO:0086005,GO:0086010,GO:0086012,GO:0086014,GO:0086015,GO:0086018,GO:0086019,GO:0086026,GO:0086046,GO:0086065,GO:0086066,GO:0086070,GO:0086091,GO:0090257,GO:0097060,GO:0097458,GO:0098590,GO:0098771,GO:0098794,GO:0098900,GO:0098901,GO:0098907,GO:0098910,GO:0099080,GO:0099081,GO:0099512,GO:0099568,GO:0099623,GO:0120025,GO:0120038,GO:1901016,GO:1901018,GO:1901019,GO:1901021,GO:1901360,GO:1901362,GO:1901379,GO:1901381,GO:1901564,GO:1901566,GO:1901576,GO:1903115,GO:1903169,GO:1903522,GO:1903779,GO:1904062,GO:1904064,GO:1904427,GO:1990778,GO:2001257,GO:2001259
Evalue2.13e-20
EggNOG OGsCOG0666@1|root,KOG4177@2759|Eukaryota
Descriptionspectrin binding
COG categoryI
BRITEko00000,ko04131
Hectar predicted targeting categoryother localisation
Exons1
Model size333
Cds size333
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815619.799332-CDS-S-firma_F_contig10782:777..11101622815619.799332-CDS-S-firma_F_contig10782:777..1110Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10782 778..1110 -
1696949458.4433744-CDS-S-firma_F_contig10782:777..11101696949458.4433744-CDS-S-firma_F_contig10782:777..1110Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10782 778..1110 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_F_contig10782.1044.1prot_S-firma_F_contig10782.1044.1Sphaerotrichia firma ET2_F femalepolypeptideS-firma_F_contig10782 778..1110 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_F_contig10782.1044.1

>prot_S-firma_F_contig10782.1044.1 ID=prot_S-firma_F_contig10782.1044.1|Name=mRNA_S-firma_F_contig10782.1044.1|organism=Sphaerotrichia firma ET2_F female|type=polypeptide|length=111bp
GLTPLHGAVILANTRPILVALLRNGANIDAKDTQGLTPLHVACMVTAERL
LRVEVVDLLLRSDADETVTDNEGRAAVDLIESGADPSGSLRRLLTNAPAD
RAWRRRGLLVL
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mRNA from alignment at S-firma_F_contig10782:778..1110-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_F_contig10782.1044.1 ID=mRNA_S-firma_F_contig10782.1044.1|Name=mRNA_S-firma_F_contig10782.1044.1|organism=Sphaerotrichia firma ET2_F female|type=mRNA|length=333bp|location=Sequence derived from alignment at S-firma_F_contig10782:778..1110- (Sphaerotrichia firma ET2_F female)
GGCTTGACGCCTCTTCACGGCGCGGTCATCCTAGCAAACACCCGACCCAT
TTTGGTGGCTCTGTTGCGCAACGGAGCCAACATCGATGCGAAAGATACCC
AGGGACTGACGCCCTTGCATGTGGCGTGCATGGTTACTGCCGAGCGCCTT
TTGAGGGTTGAAGTAGTCGACCTTCTCCTTCGGAGTGACGCAGACGAGAC
CGTCACCGACAACGAGGGCCGTGCTGCAGTAGACCTGATCGAGAGCGGGG
CCGACCCCTCAGGGAGCTTGCGACGACTGCTGACAAACGCCCCCGCGGAC
AGGGCGTGGCGCCGCCGTGGCTTGCTGGTCTTG
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Coding sequence (CDS) from alignment at S-firma_F_contig10782:778..1110-

>mRNA_S-firma_F_contig10782.1044.1 ID=mRNA_S-firma_F_contig10782.1044.1|Name=mRNA_S-firma_F_contig10782.1044.1|organism=Sphaerotrichia firma ET2_F female|type=CDS|length=666bp|location=Sequence derived from alignment at S-firma_F_contig10782:778..1110- (Sphaerotrichia firma ET2_F female)
GGCTTGACGCCTCTTCACGGCGCGGTCATCCTAGCAAACACCCGACCCAT
TTTGGTGGCTCTGTTGCGCAACGGAGCCAACATCGATGCGAAAGATACCC
AGGGACTGACGCCCTTGCATGTGGCGTGCATGGTTACTGCCGAGCGCCTT
TTGAGGGTTGAAGTAGTCGACCTTCTCCTTCGGAGTGACGCAGACGAGAC
CGTCACCGACAACGAGGGCCGTGCTGCAGTAGACCTGATCGAGAGCGGGG
CCGACCCCTCAGGGAGCTTGCGACGACTGCTGACAAACGCCCCCGCGGAC
AGGGCGTGGCGCCGCCGTGGCTTGCTGGTCTTGGGCTTGACGCCTCTTCA
CGGCGCGGTCATCCTAGCAAACACCCGACCCATTTTGGTGGCTCTGTTGC
GCAACGGAGCCAACATCGATGCGAAAGATACCCAGGGACTGACGCCCTTG
CATGTGGCGTGCATGGTTACTGCCGAGCGCCTTTTGAGGGTTGAAGTAGT
CGACCTTCTCCTTCGGAGTGACGCAGACGAGACCGTCACCGACAACGAGG
GCCGTGCTGCAGTAGACCTGATCGAGAGCGGGGCCGACCCCTCAGGGAGC
TTGCGACGACTGCTGACAAACGCCCCCGCGGACAGGGCGTGGCGCCGCCG
TGGCTTGCTGGTCTTG
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