prot_S-firma_F_contig1032.466.1 (polypeptide) Sphaerotrichia firma ET2_F female

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: D7G3T1_ECTSI (Long chain acyl-coA synthetase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3T1_ECTSI)

HSP 1 Score: 1100 bits (2846), Expect = 0.000e+0
Identity = 541/661 (81.85%), Postives = 586/661 (88.65%), Query Frame = 0

Query:    1 MSGSVWATEPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAEPFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDG-VPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALYA 660
            MSG+VWATE DADLP+K+G AG AA RPA TVF+VFQ  VENHG+ PAL  KD S G+SA +APW TLTWAQYY + LRFGKSLLSL F PHRCVNIIGFNS EWFIANMGAIAAGGIAAGIYTSNLPEACQYI NHSEAEVVVVENEAQL+KFV LA+TL SVKAIVMYRG+VP+ T+CG PVY W QFM LG  +PD+ IEERIEAQ+PGQCCSLIYTSGTTG PKAVMISHDN+TWTAETL  PFN +H+ER VSYLPLSHVAGQL+D+HAPMA   SVWFAQPDALKG+LVKTLR+VRPT FVGVPRVWEKMYEKMQE+GRS+ G K +++ W+KG+GLANAR +EY GGGGTP GF IANA+VFSKVK  LGLD+  L FT AAPIA+ETLEYFASLNIHI EVFGQSECTGPMAV+T++AWKMGSCGRPMLGTESKL P TGELCYRGRNIFMGYMHNP KTAETIDE GWMHSGD+ATFDED D RIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFK ELPCIANCMVIGDKRKFLTILIALRS + E E+G   TV+L GDSLTAC+ IGSTATT+EEAKAD KWTEYIN AMKACN RTTSNAQRVAKWA LP+DFSVEGGELTETLKL+RGP SEKYSDIIE LYA
Sbjct:    1 MSGTVWATERDADLPVKIGTAGAAA-RPAKTVFEVFQKAVENHGENPALMFKDTSGGMSADDAPWQTLTWAQYYMETLRFGKSLLSLDFAPHRCVNIIGFNSTEWFIANMGAIAAGGIAAGIYTSNLPEACQYITNHSEAEVVVVENEAQLAKFVKLAATLTSVKAIVMYRGNVPEETECGFPVYTWKQFMELGSKIPDTKIEERIEAQRPGQCCSLIYTSGTTGQPKAVMISHDNMTWTAETLCAPFNWSHAERGVSYLPLSHVAGQLLDMHAPMAVASSVWFAQPDALKGTLVKTLREVRPTTFVGVPRVWEKMYEKMQEIGRSIHGAKAALSAWSKGRGLANARRKEYSGGGGTPWGFKIANALVFSKVKEKLGLDKANLCFTTAAPIAQETLEYFASLNIHIMEVFGQSECTGPMAVSTKEAWKMGSCGRPMLGTESKLEPGTGELCYRGRNIFMGYMHNPEKTAETIDEDGWMHSGDVATFDEDGDPRIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKAELPCIANCMVIGDKRKFLTILIALRSEKREDENGGSSTVRLVGDSLTACEKIGSTATTIEEAKADTKWTEYINKAMKACNARTTSNAQRVAKWAFLPHDFSVEGGELTETLKLRRGPTSEKYSDIIEGLYA 660          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: A0A6H5KWQ0_9PHAE (AMP-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KWQ0_9PHAE)

HSP 1 Score: 1093 bits (2827), Expect = 0.000e+0
Identity = 530/660 (80.30%), Postives = 585/660 (88.64%), Query Frame = 0

Query:    1 MSGSVWATEPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAEPFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALYA 660
            MSG+VWATEP ADLPIKMG +G AA +P TTVFKVFQ  VENHG+  ALK KD S G+SA +APW TLTWAQ+Y + LRFGKSL+SLGF PHR +NIIGFNS EWFIANMGAIAAGGI AGIYTSNLPEACQYI NHSEAEVVVVENEAQL KFV LA+TL SVKAIVMYRG+VP+GTDCG PVY WDQFM LG ++PDSSI+ERIEAQKPG+CCSLIYTSGTTG PKAVMISHDN+TWTAETL   F +  + R VSYLPLSH+A Q++D+H PM  GC+V FAQPDALKGSLVKTLR++RPT+F GVPRVWEKMYEKMQEVGRS+KG KKSI+TWAKGKGLA+AR+Q+YG GGG PCGFG+ANA+ FSKVKANLGLDQ T  FT AAPIA E +EYFASLNIHI E+FGQSECTGP    T +AWK+G+CGRPM GTESKLVPETGELCYRGR+IFMGYMHNP KTAETIDE GWMHSGD+ATFDED D+R EGPSGF++ITGRIKELIITAGGEN+PPVLIE+EFKTELPCIANCMV+GDKRKFL+ILIALRS  E  EDG PT KLAGDSLTAC+ IGSTATT+EEAKADPKWTEYI+ A+KACN RTTSNAQRVAKWALLP+DFSVEGGELT TLKLKRGPASEKY+DIIE LYA
Sbjct:    1 MSGTVWATEPHADLPIKMGTSGAAA-KPPTTVFKVFQKAVENHGENVALKFKDTSGGVSADDAPWQTLTWAQFYMETLRFGKSLMSLGFAPHRSINIIGFNSTEWFIANMGAIAAGGIPAGIYTSNLPEACQYITNHSEAEVVVVENEAQLDKFVKLAATLTSVKAIVMYRGNVPEGTDCGFPVYTWDQFMELGGEIPDSSIQERIEAQKPGECCSLIYTSGTTGKPKAVMISHDNMTWTAETLTTQFPLNETSRVVSYLPLSHIAAQILDMHGPMFLGCTVAFAQPDALKGSLVKTLREIRPTVFFGVPRVWEKMYEKMQEVGRSIKGAKKSISTWAKGKGLAHARMQQYGAGGGVPCGFGVANALAFSKVKANLGLDQATCCFTGAAPIAVEIIEYFASLNIHILELFGQSECTGPQTFNTAEAWKIGTCGRPMAGTESKLVPETGELCYRGRHIFMGYMHNPEKTAETIDEDGWMHSGDVATFDEDGDDRTEGPSGFMQITGRIKELIITAGGENVPPVLIENEFKTELPCIANCMVVGDKRKFLSILIALRS--EVAEDGTPTSKLAGDSLTACEKIGSTATTIEEAKADPKWTEYIDKAVKACNNRTTSNAQRVAKWALLPHDFSVEGGELTPTLKLKRGPASEKYADIIEGLYA 657          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: A0A835Z6J1_9STRA (Long chain acyl-CoA synthetase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z6J1_9STRA)

HSP 1 Score: 832 bits (2149), Expect = 1.150e-296
Identity = 405/655 (61.83%), Postives = 502/655 (76.64%), Query Frame = 0

Query:    6 WATEPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGD-VPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAEPFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALY 659
            W TEPD  +PI+MG  G AA RP+ T+ +VF++ + N G  PALK +  ++G+   E PW+TLTW  YY + ++F K+L+SLGF PHRCVNIIGFNS EWF+A MG I AGGIAAGIYT+NLPEAC+YI +HS+AEVVVVE   QL K++ +A  LK +KAIV+Y  + +    DC +PVY W  F  LGKDV D+ +EER+  Q+PG CC LIYTSGTTG PKAVM+SHDNITWTA+T    F+    +RS+SYLPLSH+A Q++D++ PM  G  V+FAQPDAL+GSL  TL+ VRPT+F+GVPRVWEK+YEKMQEVG+   G KK IATWAK KG  + R+ +YGGGGG PCG+ +AN VVF+KVK NLGLDQ    F+AAAPI+ E LEYF SL++ I+EVFGQSECTGP  +   + W +G+CGRPM GTE+K+V +TGELCYRGR+IFMGYMH+P KTAETIDE GW+HSGD+ATFD++    +  PSGF+RITGRIKELIITAGGEN+ PVLIE+EFK  LP ++NCMV+GDKRK+LTIL++L++   E E G+PT KLAG +L   KAIGS ATTV EA   PKW E+I AA    N    S AQ + KWALLP DFS +GGELT TLKLKRGP ++K+ DII+ALY
Sbjct:    7 WVTEPDEVIPIRMGAEGVAA-RPSRTMCEVFKSALANFGTHPALKYQ--AEGI---EGPWSTLTWTDYYNESVKFAKTLMSLGFAPHRCVNIIGFNSVEWFVAEMGCILAGGIAAGIYTTNLPEACRYISDHSQAEVVVVEGALQLKKYISIAGQLKHLKAIVVYGNETLAPVPDCPVPVYTWSDFQQLGKDVGDAQVEERMAGQQPGHCCCLIYTSGTTGTPKAVMLSHDNITWTAQTALMHFSFKIGDRSISYLPLSHIAAQILDMYGPMLCGSCVYFAQPDALRGSLGATLKAVRPTLFLGVPRVWEKIYEKMQEVGKKTVGTKKKIATWAKQKGAEHTRMCQYGGGGGEPCGYNVANKVVFTKVKENLGLDQAKGCFSAAAPISTEILEYFGSLDLPIYEVFGQSECTGPHTINDPEGWLIGTCGRPMRGTENKIVADTGELCYRGRHIFMGYMHDPEKTAETIDEEGWLHSGDVATFDDNNRTDMPAPSGFMRITGRIKELIITAGGENVAPVLIENEFKKALPSLSNCMVVGDKRKYLTILLSLKTELNEAE-GIPTDKLAGTALETSKAIGSEATTVPEAATCPKWREHIEAARSKVNEEAPSRAQVIQKWALLPMDFSEKGGELTPTLKLKRGPTADKHRDIIDALY 654          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: A0A7S2K6B1_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2K6B1_9STRA)

HSP 1 Score: 715 bits (1846), Expect = 1.450e-250
Identity = 364/663 (54.90%), Postives = 468/663 (70.59%), Query Frame = 0

Query:    8 TEPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLS-KGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCG---IPVYDWDQFMGLGKD---VPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAE---PFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALYA 660
            ++P   + I++G +G AA  P T V  VFQ       + PAL  K +      AAE PW T T+ QY  Q   F KSL+SLGF  H C+NIIGFNS EWF AN G IAAGGIAAGIYT+NLPEAC Y+ +HS+A+VVVVE   QL K+  +++ L ++KA+V+Y          G   +PVY +D+F+ LGKD   V D++++  I+AQKPG+ C+LIYTSGTTG PKAVMI+HDN+TWT++ +     P  + + +  +SYLPLSH+A Q++D+H P+  GC VWFAQ DAL+GSL  TL++VRPTIF GVPRVWEKMYE MQ+V +S  GVKK I+TWAKGK  A     +YG  GG+P G+GIA+ ++  KV   LGL++    FT AAPI  + L YFAS+++ I+E+FGQSECTGP  V    AWK+G+CGRP+ GTES++ P TGEL Y GR+IF GYM+   KTA+TID GGW+HSGD+ +FD+     IEGPSGF+ ITGRIKELIITAGGEN+ PVLIESEFK  +P ++NCMVIGDKRKFL+IL+ L  + E  E+GV + KLAG++L   K IGS+ATT  E     KW +Y +  M   N +T S AQRVAKWA++P DF+ +GGELT TLKLKR  A  KYS+I+E +YA
Sbjct:    6 SDPKECVEIRIGNSGIAADEPKT-VMSVFQANCRKFANAPALHQKVMHLSDKPAAETPWTTWTFKQYEEQVFSFAKSLISLGFEKHDCINIIGFNSPEWFFANFGTIAAGGIAAGIYTTNLPEACHYVSDHSKAKVVVVEGAKQLEKYTEISARLPNLKAVVVYGEKTKPMESVGNGAVPVYLFDEFLQLGKDAADVTDTTVQASIDAQKPGETCTLIYTSGTTGNPKAVMITHDNLTWTSKNMLVNTLPRALTNDDNIISYLPLSHIAAQMLDMHCPIQCGCQVWFAQADALRGSLGTTLKEVRPTIFFGVPRVWEKMYEAMQKVAKSTTGVKKMISTWAKGKASAKNIAGQYGESGGSPWGYGIASKIL-GKVHVALGLERCLCAFTGAAPIEVKILNYFASIDLPIYELFGQSECTGPHTVNFPGAWKVGTCGRPLPGTESRMEPGTGELSYTGRHIFAGYMYMDDKTADTIDSGGWLHSGDVVSFDDCSTPGIEGPSGFMSITGRIKELIITAGGENVAPVLIESEFKLAMPALSNCMVIGDKRKFLSILLCL--LVEVDEEGVASDKLAGEALDTSKRIGSSATTTAEVMTCSKWKDYFDKGMAVANDKTISRAQRVAKWAVVPTDFTEKGGELTPTLKLKRSVAEAKYSEIVEGIYA 664          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: A0A4D9DBU9_9STRA (AMP-binding domain-containing protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9DBU9_9STRA)

HSP 1 Score: 713 bits (1841), Expect = 4.910e-250
Identity = 359/649 (55.32%), Postives = 453/649 (69.80%), Query Frame = 0

Query:   13 DLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAE--PFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALY 659
            ++ +++ K G  +  P T VF+VF+ TV+ +GD PAL  K +  G S A   W++ TW +YY   L+F KSLLSLGF  H  +N+IGFNS EW IAN GAIAAGG+  GIYTSN  +AC+YI  HSEAEVVVVEN  QL K++ +A  L  +KA+V+Y G   +G  C +P+Y W  FM LG  V D ++  RIEAQ+PG CC+LIYTSGTTGPPKAVMISHDN+TWT +      PF +   +RSVSYLPLSHVA Q++D+H P+A G  ++FAQPDAL+GSL  TL+DV PT F GVPRVWEK+YEKMQEV RS  GVK+++A WAK KGL   R Q+YG GGG P GFG A+A+V SKVKA LGL +T +  T+AAPIA E LEYFASL+I + E+FGQSECTGP       AWK+GS GR + G ++K      E C  GR+I MGYM    KT E +D  GW+HSGD+A  D D         GF  ITGRIKELIITAGGENIPPVLIE+E  + LP +ANCMV+GDK+KFLT+L+ +++  ++   G PT +L  ++L   K IGS A+T E+  +DP W +Y +  +K  N+  TSNAQ V KW +LP DFS +GGELT TLKLKR   +EKY+D+I  +Y
Sbjct:   12 EVDLRIEKEGPGSWVPKT-VFQVFEETVKKYGDSPALHYKKVPHGGSLATTEWSSYTWREYYDLTLKFCKSLLSLGFPAHGAINLIGFNSPEWLIANCGAIAAGGVGVGIYTSNGVDACKYITEHSEAEVVVVENAKQLEKYLKIAKELPRLKALVIYSGTA-EGYKCDVPIYSWKDFMALGSGVKDEAVRARIEAQRPGHCCTLIYTSGTTGPPKAVMISHDNLTWTVKNFVASLPFTLTCEDRSVSYLPLSHVAAQMLDIHCPIATGAKIYFAQPDALRGSLPVTLKDVCPTYFFGVPRVWEKIYEKMQEVARSTTGVKRALAQWAKAKGLEKNRRQQYGCGGGAPVGFGCAHALVLSKVKAALGLHETKMCITSAAPIAVEILEYFASLDIPVLELFGQSECTGPHTSNFSYAWKIGSIGRDIPGVKTKQHANMSEFCMYGRHIMMGYMKMEDKTQEAVDNEGWLHSGDVAQVDAD---------GFWSITGRIKELIITAGGENIPPVLIENEIMSALPAVANCMVVGDKKKFLTVLLTMKAKLDD--QGNPTKELNKEALDIGKEIGSNASTTEQVASDPHWKKYFDEGLKKANSTATSNAQFVQKWNVLPLDFSEKGGELTPTLKLKRSVVAEKYADVIADMY 647          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: G9BBC7_9STRA (Long-chain acyl-coenzyme A synthetase n=1 Tax=Nannochloropsis oculata TaxID=43925 RepID=G9BBC7_9STRA)

HSP 1 Score: 706 bits (1821), Expect = 5.100e-247
Identity = 361/654 (55.20%), Postives = 444/654 (67.89%), Query Frame = 0

Query:    8 TEPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETL--AEPFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALY 659
            T    ++ ++M K G  A  P T V++VF+ TV+ +GD+PAL  K +  G S     W+   W +YY   L F KSLL+L F  H  +NIIGFNS EW IAN GAIAAGG+  GIYTSN  EAC YI  HSEAEVVVVEN  QL K+V +A  L  +KA+V+Y G   +G  C  P+Y W  FM LGKD  ++++  RIEAQ+PG CC+LIYTSGTTGPPKAVMISHDN+TWT +    A PF +   +RSVSYLPL HVA Q++D+H P+A G  ++FAQ DAL+GSL  TL+D  PT F GVPRVWEK+YEKMQEV RS  GVK+++A WAK KGL   R Q+YGGGGG P GFG A A+V SKVKA LGL QT +  T+AAPI+ E LEYFASL+I + E+FGQSECTGP       AWK+GS GR + G ++K      E C  GR+I MGYM    KT E +DE GW+HSGD+A  D D         GF  ITGRIKELIITAGGENIPPVLIE+E K  L  +ANCMV+GDK+KFLT+L+ +++  +E   G PT  L  ++L   K +GS ATT E+   DP W +Y +  +K  N   T NAQ V KWA+LP DFS +GGELT TLKLKR   +EKY+D+   LY
Sbjct:    7 TSASGEVDLRMEKEGPGAWEPRT-VYQVFEETVQRYGDRPALHFKKVPHGGSPETTGWSVYAWREYYDLTLTFAKSLLALDFPAHGAINIIGFNSPEWLIANCGAIAAGGVGVGIYTSNNAEACNYISEHSEAEVVVVENAKQLEKYVKIAKNLPRLKALVVYDG-TGEGFTCDTPIYSWKAFMALGKDKSEAAVRARIEAQRPGHCCTLIYTSGTTGPPKAVMISHDNLTWTVKNFVAALPFTLTCEDRSVSYLPLPHVAAQMLDVHCPIASGAKIYFAQADALRGSLPNTLKDACPTYFFGVPRVWEKIYEKMQEVARSTTGVKRALAQWAKAKGLEKNRRQQYGGGGGAPVGFGCAYALVLSKVKAALGLHQTKICITSAAPISVEVLEYFASLDIPVLELFGQSECTGPHTSNFSYAWKIGSIGRDIPGVKTKQEAAAKEFCMFGRHIMMGYMKMEEKTKEAVDEEGWLHSGDVADVDAD---------GFWTITGRIKELIITAGGENIPPVLIETEVKAALHAVANCMVVGDKKKFLTVLLTMKTKLDE--QGNPTNALNREALDIGKELGSEATTTEQVGKDPAWKKYFDEGLKKANAAATFNAQFVQKWAVLPLDFSEKGGELTPTLKLKRSVVAEKYADVTANLY 647          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: A0A1V9YWX5_9STRA (Long-chain-fatty-acid-CoA ligase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YWX5_9STRA)

HSP 1 Score: 678 bits (1750), Expect = 5.100e-236
Identity = 350/656 (53.35%), Postives = 444/656 (67.68%), Query Frame = 0

Query:    9 EPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAEPFNMA-----HSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALY 659
            E D  + ++MG  GT AGR   TV   FQ  V  HGD+ AL  K         +  WATL+W  Y+ QC  FGKSL++LG  P   V+IIGFN+ EW IAN+G I AGGIAAGIYT+N P ACQYI  H+ A VVV +  AQL K   +A  L ++KA+V+Y   VP G  C +P + +  F+ LG      S+E R++AQ P QCC+LIYTSGTTG PKAVM+SHDNITWT   + +  ++A     H+ R VSYLPLSHVA Q++D+H P+A G  V+FAQPDALKGSL +TL++VRPT F+GVPRVWEK+ EKM ++G+  KGVKK++A WAKGKG    +L ++G  GG PCGFG+ANAVV SKV+A LGLD        AAP++ E L YF+SL+I I+++FGQSEC+GP +V    AWK+GS GRP+ GTE ++ PET EL +RGR+  MGY+   A+TA  +D  GW+HSGD AT D D         GF  ITGRIKELIITAGGENIPPVL+E   K ELP ++N MV+GDKRKFL  L+ LR V +   DG PT +L   +L     + S ATT  EA +  K   Y++A MK  N R TS AQ +AK+ +LP+DFS+ GGELT TLKLKR    +KY+++IEALY
Sbjct:   23 ERDGQVQLRMGPPGTIAGRAPETVCDAFQRVVAEHGDEDALLWKK--------DGSWATLSWRAYFEQCQAFGKSLVALGMEPFDAVSIIGFNAPEWLIANVGCILAGGIAAGIYTTNNPPACQYIAAHARAVVVVCDGVAQLEKLASIADQLPALKALVVYNAAVPAGFQCRVPCHSFADFLALGASA--GSLEARMQAQTPNQCCTLIYTSGTTGNPKAVMLSHDNITWTIAAMLQAQSVAGVGLDHTARLVSYLPLSHVAAQMIDVHLPIAIGAKVYFAQPDALKGSLGQTLKEVRPTFFLGVPRVWEKIAEKMWDIGKQTKGVKKTLAAWAKGKGARKNQLAQFGASGGAPCGFGVANAVVLSKVRAALGLDACRFCAVGAAPMSVEILTYFSSLDIPIYDIFGQSECSGPQSVCLPGAWKIGSVGRPLPGTEWRVEPETRELVFRGRHTMMGYLAMEAETAAAVDTDGWLHSGDCATIDAD---------GFGAITGRIKELIITAGGENIPPVLLEDAIKEELPILSNVMVVGDKRKFLAALLTLRVVMDG--DGAPTTQLDAKALEVLAGLQSPATTPAEAISCAKLQAYLDAGMKRANARATSRAQSIAKFHVLPHDFSMPGGELTPTLKLKRKVVVDKYAEVIEALY 657          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: T0Q1D2_SAPDV (AMP-binding domain-containing protein n=1 Tax=Saprolegnia diclina (strain VS20) TaxID=1156394 RepID=T0Q1D2_SAPDV)

HSP 1 Score: 676 bits (1745), Expect = 2.720e-235
Identity = 348/654 (53.21%), Postives = 445/654 (68.04%), Query Frame = 0

Query:   11 DADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAEP-----FNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALY 659
            D  + I+MG  GT AG+   T+   F+  V +HG + AL  K        A   WA ++W +Y+ +C  FG+SLL+LG      V+IIGFNS EW IAN+G I AGGIAAGIYT+N P ACQYI NH+ A VVV +  AQL K   +A  L ++KA+V+Y   VP+G  C +P + +D FM LG  V    ++ R+  Q+PGQC +LIYTSGTTG PKAVM+SHDNITWT   + E        + H+ R VSYLPLSHVA Q++D+H P+  G  V+FAQPDALKGSL +TL++VRPT F+GVPRVWEK+ EKM ++G+  KG+KKSIATWAK KG    +L +YG  GG PCG+G+AN++V SKV+A LGLD        AAP++ E L YF+SL+I I+++FGQSEC+GP +V    AWK+GS G+P+LGTE  ++PET EL +RGR+  MGY+   A+TA  ID  GW+HSGD AT DED         GF  ITGRIKELIITAGGENIPPVLIE   K ELP ++N MVIGDKRKFLT L+ LR V +   DG P+ +L   +L    +IGS AT   +A A  K   Y++A MK  N R TS AQ +AK+ +LP+DFS+ GGELT TLKLKR    +KY+D+IE+LY
Sbjct:   25 DGQVKIRMGPPGTIAGQAPITIIDSFKKVVADHGAEDALLWK--------ANGAWAKMSWREYFDKCQAFGRSLLALGMDRFEAVSIIGFNSPEWLIANVGCILAGGIAAGIYTTNNPPACQYIANHASAAVVVCDGVAQLEKMASIADQLPALKALVVYNAAVPNGFQCSVPCHSFDDFMALG--VKTEGLDVRMADQRPGQCSTLIYTSGTTGNPKAVMLSHDNITWTVAAMLEAQAKAGVGLDHNARLVSYLPLSHVAAQMIDIHLPIMSGAKVYFAQPDALKGSLGQTLKEVRPTFFLGVPRVWEKIAEKMWDIGKQTKGLKKSIATWAKKKGSRKNQLAQYGASGGAPCGYGLANSLVLSKVRAALGLDDCRFCAVGAAPMSVEILNYFSSLDIPIYDIFGQSECSGPQSVCFPSAWKIGSVGKPLLGTEWMVMPETRELVFRGRHTMMGYLAMEAETAAAIDTDGWLHSGDCATLDED---------GFGFITGRIKELIITAGGENIPPVLIEDVIKEELPLLSNVMVIGDKRKFLTALLTLRVVMDG--DGAPSTQLDPKALEVLASIGSNATNSNDAIACVKVQTYLDAGMKKANARATSRAQSIAKFHVLPHDFSLPGGELTPTLKLKRKVVVDKYADVIESLY 657          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: A0A485LMY3_9STRA (Aste57867_23483 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LMY3_9STRA)

HSP 1 Score: 673 bits (1737), Expect = 3.240e-234
Identity = 343/656 (52.29%), Postives = 447/656 (68.14%), Query Frame = 0

Query:    9 EPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGDVPDGTDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETLAEPF-----NMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALY 659
            E D ++ I+MG   T AGRP TTV ++FQNTV  +G++ AL SK         +  W  LTW+QYY +C  F KSL+++G      V++IGFN+ EW IA++G I AGG+AAGIYT+N P A QYI++HS A VVV +  AQL K   +A+ L  +KA+V+Y   VP+G  C +PVY +D F+ LG  V D  ++ER+++Q+PG CC+LIYTSGTTG PKAVMISHDNIT+T   + E F      +  ++R VSYLPLSHVA QL+D+H P+A G  ++FAQPDALKG+LV+TL++VRPT F+GVPRVWEK+ EK+ E+G++ KG+K +IATWAK  G   A+L +YG  GG PCG+G AN+VV SK+K  LGLDQ      AAAPI  + L YF+SL++ I+E++GQSE TGP +      WK+G+ G+ + GTE  + P   EL  RGRN+ MGY+    +T ETID+ GW+HSGD    D         P GF  ITGRIKELIITAGGEN+PPV+IE   K ELP ++N MVIGDKRKFL  L+  R   +  ++G PT KL   +L    +I STATT EEAKAD K   Y+N  +K  N R TS AQ VAK+ +L +DFS+ GGELT TLKLKR    EKY++ IE +Y
Sbjct:   11 ERDGEVKIRMGAEDTTAGRPPTTVIELFQNTVSKYGNEGALYSKK--------DGEWTILTWSQYYAKCAAFAKSLIAIGMERFEAVSVIGFNAPEWTIADLGCILAGGLAAGIYTTNNPAAAQYIIDHSGARVVVCDGVAQLEKIASVANQLPKLKALVVYNSAVPEGFQCPVPVYSFDDFLALGDAVEDRQLKERLDSQRPGHCCTLIYTSGTTGNPKAVMISHDNITFTMNAMFELFVKSGDPLTSADRLVSYLPLSHVAAQLVDIHLPLATGLKLYFAQPDALKGTLVQTLKEVRPTRFLGVPRVWEKIAEKLWEIGKTTKGIKLTIATWAKSIGTQKAQLAQYGQAGGVPCGYGCANSVVLSKIKEALGLDQCRFNVVAAAPIGLDILNYFSSLDLQIYELYGQSESTGPSSTCLNGQWKIGTVGQAIPGTEWMVDPTNEELLIRGRNVMMGYLGMEKETKETIDQEGWLHSGDCGKID---------PDGFGSITGRIKELIITAGGENVPPVVIEDILKEELPLLSNVMVIGDKRKFLAALLTFRV--QADQEGHPTNKLDKKALEIMASIASTATTTEEAKADEKVKAYLNHGLKKANGRATSRAQNVAKFHVLEHDFSLAGGELTPTLKLKRRVVVEKYAETIEEIY 647          

BLAST of mRNA_S-firma_F_contig1032.466.1 vs. uniprot
Match: B7FXX6_PHATC (Long chain acyl-coa synthetase n=3 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FXX6_PHATC)

HSP 1 Score: 671 bits (1731), Expect = 3.710e-233
Identity = 344/659 (52.20%), Postives = 452/659 (68.59%), Query Frame = 0

Query:    8 TEPDADLPIKMGKAGTAAGRPATTVFKVFQNTVENHGDKPALKSKDLSKGLSAAEAPWATLTWAQYYTQCLRFGKSLLSLGFGPHRCVNIIGFNSAEWFIANMGAIAAGGIAAGIYTSNLPEACQYIVNHSEAEVVVVENEAQLSKFVGLASTLKSVKAIVMYRGD-VPDG--TDCGIPVYDWDQFMGLGKDVPDSSIEERIEAQKPGQCCSLIYTSGTTGPPKAVMISHDNITWTAETL---AEPFNMAHSERSVSYLPLSHVAGQLMDLHAPMARGCSVWFAQPDALKGSLVKTLRDVRPTIFVGVPRVWEKMYEKMQEVGRSVKGVKKSIATWAKGKGLANARLQEYGGGGGTPCGFGIANAVVFSKVKANLGLDQTTLFFTAAAPIAKETLEYFASLNIHIFEVFGQSECTGPMAVTTRDAWKMGSCGRPMLGTESKLVPETGELCYRGRNIFMGYMHNPAKTAETIDEGGWMHSGDIATFDEDEDERIEGPSGFVRITGRIKELIITAGGENIPPVLIESEFKTELPCIANCMVIGDKRKFLTILIALRSVEEEGEDGVPTVKLAGDSLTACKAIGSTATTVEEAKADPKWTEYINAAMKACNTRTTSNAQRVAKWALLPNDFSVEGGELTETLKLKRGPASEKYSDIIEALYA 660
            +E   ++ I+MG  G A+  P  TV + F   V  +G++ AL  K   +G   A+  W   +W +Y  Q   FGK+LL +GF     +NIIGFN+ EWF AN GAIAAGGI AG+YT+N PEAC Y+  HSEA+VVV E   QL K+  ++  L ++ A+VMY  D +P+   + C +PVY ++ F+ LGKDV D+ ++ R ++ K G+ C+LIYTSGTTGPPKAVMI++DNITWT ETL        + H++  +SYLPLSH+A Q++D+H PMA G  ++FA  +ALKGSL +TL++VRPT+F GVPRVWEK+Y+K+QEV RS  G+KK ++TWAKGK  A+    EYG   G+P    +A  ++  K    LG D+   F+ +AAPI  + L+YF SL+I I E+FGQSECTGP AV   DA+K+G+ GRP++GTE+K+   TGELCYRGR+IF GYM  P KT ETID  GW+HSGDI   D D    I+ PSGF+ ITGRIKELIITAGGEN+ PVLIE E K  +P +AN MVIGDKRKFL++L+ L+ VE + E G PT KL G++L A K IGS A T  EA+ DPKW EY +  ++  N +  S A RV KWALL  DFS  GGELT T+KLKR   +EK++D I+A+YA
Sbjct:    9 SEASQEVAIRMGNEGIAS-LPPKTVMQNFDAQVTKNGNRTALYHKRPKQGQDVADVAWTKYSWKEYRQQVDSFGKALLKVGFARFDTINIIGFNAPEWFFANFGAIAAGGIPAGVYTTNNPEACAYVAEHSEAKVVVCEGVKQLEKYYEISRNLPNLTALVMYGTDSIPEDVKSKCSVPVYTFEGFLDLGKDVSDTDLKARTDSWKAGETCTLIYTSGTTGPPKAVMITNDNITWTIETLMGRTRKGTLDHNDVMISYLPLSHIAAQMLDMHNPMATGTQLYFADANALKGSLGQTLKEVRPTVFFGVPRVWEKIYDKLQEVARSSTGIKKMLSTWAKGKAAAHWESLEYGSKSGSPFMLFLAKKLLH-KAHLALGFDRCIQFYVSAAPIEVKILKYFMSLDIPIMELFGQSECTGPHAVNGYDAFKVGTVGRPLIGTETKIDEATGELCYRGRHIFAGYMGMPDKTTETIDSQGWLHSGDIVAIDSDHHPEIKKPSGFISITGRIKELIITAGGENVAPVLIEDELKAAMPALANAMVIGDKRKFLSVLLCLQ-VEPDIE-GNPTNKLTGNALEAAKTIGSKAKTTNEARDDPKWKEYFDKGLEVANKKAISRASRVGKWALLSTDFSEVGGELTPTMKLKRNVTAEKFADTIDAMYA 663