mRNA_S-firma_F_contig11100.1463.1 (mRNA) Sphaerotrichia firma ET2_F female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_F_contig11100.1463.1
Unique NamemRNA_S-firma_F_contig11100.1463.1
TypemRNA
OrganismSphaerotrichia firma ET2_F female (Sphaerotrichia firma ET2_F female)
Homology
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: A0A812Y096_9DINO (RFC5 protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812Y096_9DINO)

HSP 1 Score: 139 bits (350), Expect = 1.940e-37
Identity = 74/135 (54.81%), Postives = 92/135 (68.15%), Query Frame = 1 
Query:   13 GIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAFVHVRYM 417
            GI A+LRL+ GDMRRVLNILQAT+M+   + Q+ VY+ TGDPLPADIR    +++N  F EA +    + V KG +L DILT L TL+  + F    +AYL+  LADIEYRLAS   + LQMASLV AFV  R M
Sbjct:  195 GIKAVLRLAQGDMRRVLNILQATAMSSGTITQEAVYATTGDPLPADIRAAAGALLNKPFDEALQTLQDLAVHKGLSLADILTNLVTLVNAMGFPPAVRAYLLEKLADIEYRLASCMDENLQMASLVSAFVLARDM 329
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: UPI00115E6E32 (replication factor C subunit 5 n=1 Tax=Manacus vitellinus TaxID=328815 RepID=UPI00115E6E32)

HSP 1 Score: 133 bits (334), Expect = 3.880e-37
Identity = 67/133 (50.38%), Postives = 93/133 (69.92%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAF 399
            VT+DG+ A++ LS GDMRR LNILQ+TSMA+ +V ++ VY+CTG PL +DI  +L  M+N  FS A  K   +   KG AL+DILTEL   + R+ F    +  L+  +ADIEYRLA+ TS+K+Q++SL+ AF
Sbjct:    5 VTEDGLKALVTLSSGDMRRALNILQSTSMAFGKVTEENVYTCTGHPLKSDIANILDWMLNQDFSTAYRKITELKTLKGLALQDILTELHLFVHRVDFPPSIRIQLLIKMADIEYRLAAGTSEKIQLSSLIAAF 137
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: A0A7S2W057_9STRA (Hypothetical protein n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2W057_9STRA)

HSP 1 Score: 138 bits (348), Expect = 4.190e-37
Identity = 67/139 (48.20%), Postives = 102/139 (73.38%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAFVHVRYM 417
            +++ GI+AIL LSGGDMRRVLN+LQ+T+M++  +++ +VY  +G PLP+DI T+  S+ ND F +A E   TMC +KGYA+ DILT+L+T++T +   +   A L+  +++IEYRLA  T +K+Q ASLVG F+  R++
Sbjct:  196 ISEGGIEAILSLSGGDMRRVLNLLQSTAMSFETIDETSVYLTSGAPLPSDIDTITNSLFNDDFVKAYENLITMCSTKGYAMADILTDLTTIVTTMKLPSGVLADLLDGMSNIEYRLAYGTDEKIQGASLVGVFIKARHL 334
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: A0A7K4LIK8_9AVES (RFC5 factor (Fragment) n=1 Tax=Crypturellus undulatus TaxID=48396 RepID=A0A7K4LIK8_9AVES)

HSP 1 Score: 132 bits (332), Expect = 7.350e-37
Identity = 67/133 (50.38%), Postives = 92/133 (69.17%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAF 399
            VT+DG+ A++ LS GDMRR LNILQ+TSMA+  V ++ VY+CTG PL  DI  +L  M+N  FS A  K   +   KG AL DILTE+  L+ R+ F    +  L++ +ADIEYRLA+ TS+K+Q++SL+ AF
Sbjct:    3 VTEDGMKALVTLSSGDMRRALNILQSTSMAFGTVTEENVYTCTGHPLKCDIANILDWMLNQDFSTAYRKISELKTLKGLALHDILTEIHLLVHRVDFPPSIRIQLLSKMADIEYRLAAGTSEKIQLSSLIAAF 135
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: A0A672I8B0_SALFA (Rep_fac_C domain-containing protein n=2 Tax=Percomorphaceae TaxID=1489872 RepID=A0A672I8B0_SALFA)

HSP 1 Score: 132 bits (331), Expect = 1.280e-36
Identity = 69/137 (50.36%), Postives = 95/137 (69.34%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAFVHVR 411
            +T DG+ AI+ LS GDMRR LNILQ+TSMAY +V +DTVY+CTG PL +DI  +L   +N  F+ A ++   +   KG AL DILTE+  L+ R+ F    +  L+  LAD+E+RLAS TS+K+Q++S+V AF  VR
Sbjct:    9 ITPDGMKAIVTLSSGDMRRSLNILQSTSMAYEKVTEDTVYTCTGHPLRSDIANILDWCLNKDFTTAYKQILHLKTLKGLALNDILTEIHLLVHRVDFPPAVRISLLIKLADVEHRLASGTSEKIQLSSMVAAFQAVR 145
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: A0A7K5BIQ4_9FURN (RFC5 factor (Fragment) n=1 Tax=Furnarius figulus TaxID=463165 RepID=A0A7K5BIQ4_9FURN)

HSP 1 Score: 132 bits (331), Expect = 4.950e-36
Identity = 66/133 (49.62%), Postives = 93/133 (69.92%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAF 399
            VT+DG+ A++ LS GDMRR LNILQ+TSMA+ +V ++ VY+CTG PL +DI  +L  M+N  FS A  K   +   KG AL+DILTEL   + R+ F    +  L+  +ADIEYRLA+ T++K+Q++SL+ AF
Sbjct:   57 VTEDGMKALVTLSSGDMRRALNILQSTSMAFGKVTEENVYTCTGHPLKSDIANILDWMLNQDFSTAYHKIMELKTLKGLALQDILTELHLFVHRVDFPPSIRIQLLIKMADIEYRLAAGTNEKIQLSSLIAAF 189
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: UPI000FCD494C (replication factor C subunit 5 n=1 Tax=Neopelma chrysocephalum TaxID=114329 RepID=UPI000FCD494C)

HSP 1 Score: 133 bits (334), Expect = 5.830e-36
Identity = 67/133 (50.38%), Postives = 93/133 (69.92%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAF 399
            VT+DG+ A++ LS GDMRR LNILQ+TSMA+ +V ++ VY+CTG PL +DI  +L  M+N  FS A  K   +   KG AL+DILTEL   + R+ F    +  L+  +ADIEYRLA+ TS+K+Q++SL+ AF
Sbjct:  102 VTEDGLKALVTLSSGDMRRALNILQSTSMAFGKVTEENVYTCTGHPLKSDIANILDWMLNQDFSTAYRKITELKTLKGLALQDILTELHLFVHRVDFPPSIRIQLLIKMADIEYRLAAGTSEKIQLSSLIAAF 234
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: UPI001B7E8105 (replication factor C subunit 5-like n=3 Tax=Acipenseroidei TaxID=186622 RepID=UPI001B7E8105)

HSP 1 Score: 135 bits (340), Expect = 5.920e-36
Identity = 70/139 (50.36%), Postives = 95/139 (68.35%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAFVHVRYM 417
            VT DG+ AI+ LS GDMRR LNILQ+TSMAY  V++DTVY+CTG PL ADI  +L  M+N  FS A      +   KG AL D+LTE+  L+ R+ F    + +L+  +A+IE+RLAS TS+K+Q++S++ AF   R M
Sbjct:  191 VTPDGMKAIVTLSNGDMRRSLNILQSTSMAYGTVSEDTVYTCTGHPLKADIANILDWMLNQDFSTAYNNIMELKTLKGLALHDVLTEIHLLVHRVDFPASVRIHLLIKMANIEHRLASGTSEKIQLSSMIAAFQVARDM 329
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: UPI000FD66B6F (replication factor C subunit 5 n=1 Tax=Corapipo altera TaxID=415028 RepID=UPI000FD66B6F)

HSP 1 Score: 133 bits (334), Expect = 7.510e-36
Identity = 67/133 (50.38%), Postives = 93/133 (69.92%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAF 399
            VT+DG+ A++ LS GDMRR LNILQ+TSMA+ +V ++ VY+CTG PL +DI  +L  M+N  FS A  K   +   KG AL+DILTEL   + R+ F    +  L+  +ADIEYRLA+ TS+K+Q++SL+ AF
Sbjct:  112 VTEDGLKALVTLSSGDMRRALNILQSTSMAFGKVTEENVYTCTGHPLKSDIANILDWMLNQDFSTAYRKITELKTLKGLALQDILTELHLFVHRVDFPPSIRIQLLIKMADIEYRLAAGTSEKIQLSSLIAAF 244
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BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Match: A0A3B3HT69_ORYLA (Replication factor C subunit 5 n=31 Tax=Clupeocephala TaxID=186625 RepID=A0A3B3HT69_ORYLA)

HSP 1 Score: 135 bits (339), Expect = 8.500e-36
Identity = 68/137 (49.64%), Postives = 96/137 (70.07%), Query Frame = 1 
Query:    1 VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPADIRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTEGKAYLMATLADIEYRLASATSDKLQMASLVGAFVHVR 411
            +T+DG+ AI+ LS GDMRR LN+LQ+TSMAY +V +D+VY+CTG PL +DI  +L   +N  F+ A ++   +   KG AL DILTEL  L+ R+ F    +  L+  LAD+E+RLAS T +K+Q++S+VGAF  VR
Sbjct:  191 ITEDGMKAIVTLSSGDMRRSLNVLQSTSMAYGKVTEDSVYTCTGQPLRSDIANILDWCLNKDFTSAYKQILQLKTLKGLALHDILTELHLLVHRVDFPPATRMTLLVKLADVEHRLASGTDEKIQLSSMVGAFQEVR 327
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_F_contig11100.1463.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A812Y096_9DINO1.940e-3754.81RFC5 protein (Fragment) n=1 Tax=Symbiodinium sp. K... [more]
UPI00115E6E323.880e-3750.38replication factor C subunit 5 n=1 Tax=Manacus vit... [more]
A0A7S2W057_9STRA4.190e-3748.20Hypothetical protein n=1 Tax=Eucampia antarctica T... [more]
A0A7K4LIK8_9AVES7.350e-3750.38RFC5 factor (Fragment) n=1 Tax=Crypturellus undula... [more]
A0A672I8B0_SALFA1.280e-3650.36Rep_fac_C domain-containing protein n=2 Tax=Percom... [more]
A0A7K5BIQ4_9FURN4.950e-3649.62RFC5 factor (Fragment) n=1 Tax=Furnarius figulus T... [more]
UPI000FCD494C5.830e-3650.38replication factor C subunit 5 n=1 Tax=Neopelma ch... [more]
UPI001B7E81055.920e-3650.36replication factor C subunit 5-like n=3 Tax=Acipen... [more]
UPI000FD66B6F7.510e-3650.38replication factor C subunit 5 n=1 Tax=Corapipo al... [more]
A0A3B3HT69_ORYLA8.500e-3649.64Replication factor C subunit 5 n=31 Tax=Clupeoceph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_F_contig11100contigS-firma_F_contig11100:1321..1799 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma ET2_F female2021-02-24
Properties
Property NameValue
Seed ortholog181119.XP_005524560.1
Preferred nameRFC5
PFAMsAAA,DNA_pol3_delta2,Rep_fac_C
Max annot lvl33208|Metazoa
KEGG koko:K07915,ko:K10756
KEGG Pathwayko03030,ko03420,ko03430,map03030,map03420,map03430
KEGG ModuleM00289,M00295
GOsGO:0000723,GO:0000731,GO:0000819,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005657,GO:0005663,GO:0005694,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006271,GO:0006272,GO:0006275,GO:0006281,GO:0006283,GO:0006289,GO:0006296,GO:0006297,GO:0006301,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007049,GO:0007059,GO:0007062,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010556,GO:0010557,GO:0010604,GO:0016043,GO:0016070,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0019725,GO:0019899,GO:0019985,GO:0022402,GO:0022616,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031390,GO:0031391,GO:0031974,GO:0031981,GO:0032200,GO:0032201,GO:0032991,GO:0033260,GO:0033554,GO:0033683,GO:0034641,GO:0034645,GO:0034654,GO:0042276,GO:0042592,GO:0042769,GO:0043085,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044093,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044446,GO:0044464,GO:0044786,GO:0045935,GO:0046483,GO:0048518,GO:0048522,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051052,GO:0051054,GO:0051171,GO:0051173,GO:0051276,GO:0051338,GO:0051347,GO:0051606,GO:0051716,GO:0060249,GO:0060255,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070987,GO:0071704,GO:0071840,GO:0071897,GO:0080090,GO:0090304,GO:0090305,GO:0090329,GO:0098813,GO:1900262,GO:1900264,GO:1901360,GO:1901362,GO:1901576,GO:1902494,GO:1904949,GO:2000112,GO:2000278,GO:2000573
Evalue4.82e-36
EggNOG OGsCOG0470@1|root,KOG0990@2759|Eukaryota,38D9K@33154|Opisthokonta,3BH5A@33208|Metazoa,3CT1P@33213|Bilateria,48AFB@7711|Chordata,491KX@7742|Vertebrata,4GN04@8782|Aves
Descriptionreplication factor C
COG categoryL
BRITEko00000,ko00001,ko00002,ko03032,ko03036,ko03400,ko04031
Hectar predicted targeting categoryother localisation
Exons2
Model size435
Cds size435
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815660.069755-CDS-S-firma_F_contig11100:1320..15251622815660.069755-CDS-S-firma_F_contig11100:1320..1525Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig11100 1321..1525 +
1696949485.3318157-CDS-S-firma_F_contig11100:1320..15251696949485.3318157-CDS-S-firma_F_contig11100:1320..1525Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig11100 1321..1525 +
1622815660.0831892-CDS-S-firma_F_contig11100:1569..17991622815660.0831892-CDS-S-firma_F_contig11100:1569..1799Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig11100 1570..1799 +
1696949485.345049-CDS-S-firma_F_contig11100:1569..17991696949485.345049-CDS-S-firma_F_contig11100:1569..1799Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig11100 1570..1799 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_F_contig11100.1463.1prot_S-firma_F_contig11100.1463.1Sphaerotrichia firma ET2_F femalepolypeptideS-firma_F_contig11100 1321..1799 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_F_contig11100.1463.1

>prot_S-firma_F_contig11100.1463.1 ID=prot_S-firma_F_contig11100.1463.1|Name=mRNA_S-firma_F_contig11100.1463.1|organism=Sphaerotrichia firma ET2_F female|type=polypeptide|length=145bp
VTKDGIDAILRLSGGDMRRVLNILQATSMAYSEVNQDTVYSCTGDPLPAD
IRTMLKSMMNDTFSEANEKCWTMCVSKGYALRDILTELSTLITRLAFSTE
GKAYLMATLADIEYRLASATSDKLQMASLVGAFVHVRYMARNRS*
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mRNA from alignment at S-firma_F_contig11100:1321..1799+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_F_contig11100.1463.1 ID=mRNA_S-firma_F_contig11100.1463.1|Name=mRNA_S-firma_F_contig11100.1463.1|organism=Sphaerotrichia firma ET2_F female|type=mRNA|length=479bp|location=Sequence derived from alignment at S-firma_F_contig11100:1321..1799+ (Sphaerotrichia firma ET2_F female)
GTGACGAAAGACGGCATTGACGCCATTCTGCGCCTCAGTGGCGGCGACAT
GCGGAGGGTGTTGAACATCCTACAGGCAACGTCCATGGCGTACTCTGAAG
TGAACCAGGACACGGTGTACTCGTGCACCGGTGACCCCCTCCCCGCTGAC
ATCCGTACCATGCTCAAGTCCATGATGAACGACACGTTTTCGGAGGCCAA
CGAGAGTACGCATTGCCCCCCCATTCCCGCGTTTCTAACCCACCCCCAGA
GTGCTGGACCATGTGTGTGTCCAAGGGCTACGCGCTGCGGGACATCCTGA
CTGAGTTGTCCACGCTGATTACCCGGCTGGCGTTCAGCACGGAGGGCAAA
GCGTACCTGATGGCCACCCTGGCAGACATTGAGTACCGGTTGGCGTCCGC
AACGTCCGACAAGCTACAAATGGCGTCCCTGGTGGGCGCCTTTGTGCACG
TGCGGTACATGGCCCGCAACAGAAGCTGA
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Coding sequence (CDS) from alignment at S-firma_F_contig11100:1321..1799+

>mRNA_S-firma_F_contig11100.1463.1 ID=mRNA_S-firma_F_contig11100.1463.1|Name=mRNA_S-firma_F_contig11100.1463.1|organism=Sphaerotrichia firma ET2_F female|type=CDS|length=870bp|location=Sequence derived from alignment at S-firma_F_contig11100:1321..1799+ (Sphaerotrichia firma ET2_F female)
GTGACGAAAGACGGCATTGACGCCATTCTGCGCCTCAGTGGCGGCGACAT
GCGGAGGGTGTTGAACATCCTACAGGCAACGTCCATGGCGTACTCTGAAG
TGAACCAGGACACGGTGTACTCGTGCACCGGTGACCCCCTCCCCGCTGAC
ATCCGTACCATGCTCAAGTCCATGATGAACGACACGTTTTCGGAGGCCAA
CGAGAGTGACGAAAGACGGCATTGACGCCATTCTGCGCCTCAGTGGCGGC
GACATGCGGAGGGTGTTGAACATCCTACAGGCAACGTCCATGGCGTACTC
TGAAGTGAACCAGGACACGGTGTACTCGTGCACCGGTGACCCCCTCCCCG
CTGACATCCGTACCATGCTCAAGTCCATGATGAACGACACGTTTTCGGAG
GCCAACGAGAAGTGCTGGACCATGTGTGTGTCCAAGGGCTACGCGCTGCG
GGACATCCTGACTGAGTTGTCCACGCTGATTACCCGGCTGGCGTTCAGCA
CGGAGGGCAAAGCGTACCTGATGGCCACCCTGGCAGACATTGAGTACCGG
TTGGCGTCCGCAACGTCCGACAAGCTACAAATGGCGTCCCTGGTGGGCGC
CTTTGTGCACGTGCGGTACATGGCCCGCAACAGAAGCTGAAGTGCTGGAC
CATGTGTGTGTCCAAGGGCTACGCGCTGCGGGACATCCTGACTGAGTTGT
CCACGCTGATTACCCGGCTGGCGTTCAGCACGGAGGGCAAAGCGTACCTG
ATGGCCACCCTGGCAGACATTGAGTACCGGTTGGCGTCCGCAACGTCCGA
CAAGCTACAAATGGCGTCCCTGGTGGGCGCCTTTGTGCACGTGCGGTACA
TGGCCCGCAACAGAAGCTGA
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