mRNA_S-firma_F_contig1019.296.1 (mRNA) Sphaerotrichia firma ET2_F female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_F_contig1019.296.1
Unique NamemRNA_S-firma_F_contig1019.296.1
TypemRNA
OrganismSphaerotrichia firma ET2_F female (Sphaerotrichia firma ET2_F female)
Homology
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BLAST of mRNA_S-firma_F_contig1019.296.1 vs. uniprot
Match: A0A6H5KNU3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNU3_9PHAE)

HSP 1 Score: 85.1 bits (209), Expect = 1.220e-13
Identity = 93/270 (34.44%), Postives = 121/270 (44.81%), Query Frame = 3 
Query:  612 ARGARVNAADKEGGTPLCRAAKHGRLAVVQALLAGLTKPDACT---AWYGRSRLLPNTTMR-TSC--ERFIESGSDVNYVDIDGRTALQSAVLCLGSEVIDVLVEAAGASLEQRNVMGCAPLHIAVRKEGRLEAALALLRNGADVNVARTGFKEGEMPLHTAARRGYLVPQGRGRAGGTWHHRGXXXXXXXXXXXXXXXXRRGHRAAVDISDADFNG---PRDLQEVERARKFLVNASADRAWRRRGHLVLCRAHPERAQLRLESGQSDA 1394
            A GA+ N  D +G TPL  A ++G L+ V+ALLA    PD       W     +L     +  +C     ++ G DVN    DG T+L  A L      IDVLVEA GA +E  +   C P   A       EAALAL ++GA++         GE PL  AAR+        GR G T                         R A ++          P + Q++ER R  L NA ADRAWRRRG LVLCRA   +++ RL +  S A
Sbjct:  108 AEGAKKNGLDAQGRTPLHLAVENGHLSTVKALLAA--DPDTSVHYRIWNRSYSVLDFAAQKGDACVLRAILQHGVDVNR-RTDGYTSLHVAALNNRVAAIDVLVEA-GAHVEVEDDDDCTPFLDACSNHCH-EAALALFKHGANIEARDM---RGETPLIIAARQA-------GRQGVT-----AIVDMLLRCGADETALNPDGRTAAEVVGHHIEYAACPLNKQDLERVRDLLENAPADRAWRRRGLLVLCRAFWIKSR-RLRAASSSA 356
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BLAST of mRNA_S-firma_F_contig1019.296.1 vs. uniprot
Match: D7G6X8_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6X8_ECTSI)

HSP 1 Score: 84.7 bits (208), Expect = 1.370e-13
Identity = 107/340 (31.47%), Postives = 145/340 (42.65%), Query Frame = 3 
Query:  612 ARGARVNAADKEGGTPLCRAAKHGRLAVVQALLAGLTKPDACTAW--YGRSRLLPNTTMRT--SC--ERFIESGSDVNYVDIDGRTALQSAVLCLGSEVIDVLVEAAGASLEQRNVMGCAPLHIAVRKEGRLEAALALLRNGADVNVARTGFKEGEMPLHTAARRGYLVPQGRGRAGGTWHHRGXXXXXXXXXXXXXXXXRRGHRAAVDISDADFNG---PRDLQEVERARKFLVNASADRAWRRRGHLVLCRAHPERAQLRLESGQSDAGMATGRLVAALAWQG*RGVQASIAEAPEKVAS*RMVATLAEQRLGR*G-------WRRTACSGQSLGTCE 1583
            A GA+ N  D +G TPL  A ++G L+ V+ALLA    PD    +  + RS  + +   +   +C     ++ G DVN    DG T+L  A L      IDVLVEA GA +E  +   C P   A       EAALAL ++G+++         GE PL  AAR+        GR G T                         R A ++          P + Q+VER R  L NA  DRAWRRRG LVLCRA   +A+ RL +  S+A     R+ +        G    + E  +K    +    L    +G          WRR  CS  S  +CE
Sbjct:   59 AGGAKKNGLDAQGRTPLHLAVENGHLSTVKALLAA--DPDTSVRYRIWNRSYSVLDLAAQKGDACVLRAILQHGVDVNR-RTDGYTSLHVAALNNRVAAIDVLVEA-GAHVEVEDDDDCTPFLDACINHCH-EAALALYKHGSNIEARDI---RGETPLIIAARQA-------GRQGVT-----AIVDMLLRCGADETALNPDGRTAAEVVGHHIEYAACPLNKQDVERVRDLLENAPVDRAWRRRGFLVLCRAFWIKAR-RLRAASSNASSNGPRVRSLSTGARISGAGGELREDDDKGRR-KQGCGLVGGGVGERNVQAKCWSWRRRVCSAPSWVSCE 376
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BLAST of mRNA_S-firma_F_contig1019.296.1 vs. uniprot
Match: A0A6H5JHY9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHY9_9PHAE)

HSP 1 Score: 75.5 bits (184), Expect = 2.410e-10
Identity = 66/179 (36.87%), Postives = 83/179 (46.37%), Query Frame = 3 
Query:  813 GSDVNYVDIDGRTALQSAVLCLGSEVIDVLVEAAGASLEQRNVMGCAPLHIAVRKEGRLEAALALLRNGADVNVARTGFKEGEMPLHTAARRGYLVPQGRGRAGGTWHHRGXXXXXXXXXXXXXXXXRRGHRAAVDISDADFNGPRDLQEVERARKFLVNASADRAWRRRGHLVLCRAH 1349
            G DVN    +G TAL+ A     +  IDVL EA GA++E +   G  PLH+A  +EGRL A +ALLR+GA V          + PLH  A +      G          RG                  GH AA  +           ++V+R RK L  A ADRAWRRRG L+L RAH
Sbjct:  547 GGDVNAKVNNGITALRVASAFDKAPAIDVLTEA-GANIEAQ-ANGSTPLHVA-SQEGRLAAGIALLRHGASVGKKNAAA---QTPLHLVAAK--AGSPGTAEMVDLLLRRGADETAVS---------ENGHTAADMVVLKTEESDSLAEDVDRVRKLLARAPADRAWRRRGFLILSRAH 708
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BLAST of mRNA_S-firma_F_contig1019.296.1 vs. uniprot
Match: D7FKL4_ECTSI (Ankyrin repeat protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FKL4_ECTSI)

HSP 1 Score: 71.2 bits (173), Expect = 2.050e-9
Identity = 65/190 (34.21%), Postives = 81/190 (42.63%), Query Frame = 3 
Query:  804 IESGSDVNYVDIDGRTALQSAVLCLGSEVIDVLVEAAGASLEQRNVMGCAPLHIAVRKEGRLEAAL--ALLRNGADVNVARTGFKEGEMPLHTAARRGYLVPQGRGRAGGTWHHRGXXXXXXXXXXXXXXXXRRGHRAAVDISDADFNGPRDLQEVERARKFLVNASADRAWRRRGHLVLCRAHPERAQL 1367
            IE G+DV+  +  G  AL  A      E +DVL+ A GA+    +  G   LH+A     R EAA    LL++GADVN    G       + T A +  +V                                 G RAA  I        R  +E E  RK L NA ADRAWRRR  LVLCRA P++ QL
Sbjct:  180 IEHGADVDATNYGGVAALHHATANNKVEAMDVLLRA-GANARSMSESGHTVLHMAAITRTRTEAATLAVLLKHGADVNAQTVGGTSVLEYVATRAGKQGIVE--------------VVDLLLRWGADETHVAGNGMRAADAIGMKVPEEDRIPEEFEGVRKLLANAPADRAWRRRCSLVLCRARPDKMQL 354
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BLAST of mRNA_S-firma_F_contig1019.296.1 vs. uniprot
Match: D7G1U1_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1U1_ECTSI)

HSP 1 Score: 57.8 bits (138), Expect = 4.410e-5
Identity = 31/53 (58.49%), Postives = 35/53 (66.04%), Query Frame = 3 
Query: 1251 DLQEVERARKFLVNASADRAWRRRGHLVLCRAHPERAQLRLESGQSDAGMATG 1409
            D ++ ERAR  L  A ADRAWRRRG LVLCRAHP+R QL    G   AG+  G
Sbjct:  326 DPEDDERARSLLARAPADRAWRRRGLLVLCRAHPDRLQL----GGDLAGLVAG 374
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_F_contig1019.296.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
A0A6H5KNU3_9PHAE1.220e-1334.44Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G6X8_ECTSI1.370e-1331.47Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
A0A6H5JHY9_9PHAE2.410e-1036.87Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FKL4_ECTSI2.050e-934.21Ankyrin repeat protein n=2 Tax=Ectocarpus TaxID=28... [more]
D7G1U1_ECTSI4.410e-558.49Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_F_contig1019contigS-firma_F_contig1019:16466..19605 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma ET2_F female2021-02-24
Properties
Property NameValue
Seed ortholog2880.D7G6X8
PFAMsAnk_2,Ank_4
Max annot lvl2759|Eukaryota
Evalue1.91e-15
EggNOG OGsCOG0666@1|root,KOG4177@2759|Eukaryota
Descriptionspectrin binding
COG categoryI
Hectar predicted targeting categoryother localisation
Exons2
Model size2199
Cds size897
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_F_contig1019.296.1prot_S-firma_F_contig1019.296.1Sphaerotrichia firma ET2_F femalepolypeptideS-firma_F_contig1019 18164..19060 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815546.895661-UTR-S-firma_F_contig1019:16465..170741622815546.895661-UTR-S-firma_F_contig1019:16465..17074Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig1019 16466..17074 -
1696949406.7332861-UTR-S-firma_F_contig1019:16465..170741696949406.7332861-UTR-S-firma_F_contig1019:16465..17074Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig1019 16466..17074 -
1622815546.908528-UTR-S-firma_F_contig1019:18015..181631622815546.908528-UTR-S-firma_F_contig1019:18015..18163Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig1019 18016..18163 -
1696949406.748797-UTR-S-firma_F_contig1019:18015..181631696949406.748797-UTR-S-firma_F_contig1019:18015..18163Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig1019 18016..18163 -
1622815546.9367743-UTR-S-firma_F_contig1019:19060..196051622815546.9367743-UTR-S-firma_F_contig1019:19060..19605Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig1019 19061..19605 -
1696949406.77837-UTR-S-firma_F_contig1019:19060..196051696949406.77837-UTR-S-firma_F_contig1019:19060..19605Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig1019 19061..19605 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815546.923571-CDS-S-firma_F_contig1019:18163..190601622815546.923571-CDS-S-firma_F_contig1019:18163..19060Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig1019 18164..19060 -
1696949406.7612355-CDS-S-firma_F_contig1019:18163..190601696949406.7612355-CDS-S-firma_F_contig1019:18163..19060Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig1019 18164..19060 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_F_contig1019.296.1

>prot_S-firma_F_contig1019.296.1 ID=prot_S-firma_F_contig1019.296.1|Name=mRNA_S-firma_F_contig1019.296.1|organism=Sphaerotrichia firma ET2_F female|type=polypeptide|length=299bp
MPRDQPHSMLVPRGGKPELPTAARGARVNAADKEGGTPLCRAAKHGRLAV
VQALLAGLTKPDACTAWYGRSRLLPNTTMRTSCERFIESGSDVNYVDIDG
RTALQSAVLCLGSEVIDVLVEAAGASLEQRNVMGCAPLHIAVRKEGRLEA
ALALLRNGADVNVARTGFKEGEMPLHTAARRGYLVPQGRGRAGGTWHHRG
GGRPPASMGSRRNDRRRRGHRAAVDISDADFNGPRDLQEVERARKFLVNA
SADRAWRRRGHLVLCRAHPERAQLRLESGQSDAGMATGRLVAALAWQG*
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mRNA from alignment at S-firma_F_contig1019:16466..19605-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_F_contig1019.296.1 ID=mRNA_S-firma_F_contig1019.296.1|Name=mRNA_S-firma_F_contig1019.296.1|organism=Sphaerotrichia firma ET2_F female|type=mRNA|length=3140bp|location=Sequence derived from alignment at S-firma_F_contig1019:16466..19605- (Sphaerotrichia firma ET2_F female)
ACTTGTAGTACCAGCTGCTTGTGTACGTCGACAGGCAGCAGACAGATCAC
GTGGAAGCGCTTTTTGCTTGGGTTTTTGCCACACACACCTCCCACCTCCA
CCGCGGCCCAGGGCGAAGACACACCGCTGTCCCTGCTTCGTGGTCCATCG
GACCTTGCTACGGCGGTTTGGCCGCGCGCCTGCCTCCACATATCCACCAG
CTAGGTTACGAAGGAGAAAGCACACCGCTGCTGCCCTGCCATACTACGGC
GAGCATGACCAATAACAACAGCAGCAGCCCGCTCGAAGCTCTCCGAGGAG
ATGCGGAGCGCATCTTCAAGCTCGTATGTATTGAAACCGGGACCACACCT
GAACAGCAGTGAATGGGGCGACCGCTAGGGCTCCGCTCGAGTGCGCGGTG
TTGCGGGGAGATGAAGGCATGGTGCACAAGATCTTGGGGGCTGGTGCCAG
TATGGGAGCCGCGGTGCATGCTGCTGTTGCAAGCGGCCGTGAGCAAGTGG
TGGGTTATCTGCTGACGCAAGGAGGACGTGTTATGACAGGGATGCATGCC
TCGGGATCAACCCCACTCCATGCTGGTGCCCAGAGGCGGAAAACCGGAGC
TACCAACAGCAGCCCGGGGTGCTAGAGTAAACGCAGCAGACAAGGAAGGT
GGTACGCCGCTGTGCAGGGCAGCCAAGCATGGGCGTCTGGCCGTGGTGCA
AGCTCTCCTGGCTGGGCTGACGAAACCCGACGCTTGCACAGCTTGGTACG
GCCGCTCGAGATTGCTGCCGAACACGACCATGCGGACGTCTTGCGAGCGT
TTTATCGAGAGCGGCTCCGACGTGAACTATGTTGACATCGACGGCAGGAC
GGCGCTTCAGAGCGCCGTGCTCTGCCTCGGTTCGGAGGTGATCGATGTAC
TTGTCGAGGCCGCCGGGGCCAGCCTCGAACAAAGGAATGTGATGGGATGC
GCCCCGCTGCACATTGCTGTTAGGAAGGAGGGCCGTCTTGAGGCCGCGCT
CGCCTTGCTGAGGAATGGTGCAGACGTCAACGTGGCGCGAACGGGGTTCA
AAGAAGGAGAAATGCCGTTGCATACGGCAGCACGCAGGGGGTACCTGGTA
CCGCAGGGCCGCGGGCGGGCAGGGGGTACCTGGCACCACCGCGGAGGTGG
TAGACCTCCTGCTTCGATGGGGAGCCGACGAAACGATCGTCGACGAAGAG
GGCACAGAGCCGCCGTTGATATTTCGGACGCCGACTTCAACGGCCCGCGC
GACCTGCAGGAGGTTGAGCGTGCGCGGAAGTTCCTGGTCAACGCTTCTGC
TGACAGGGCATGGCGTCGCCGCGGCCACCTGGTCTTGTGCCGTGCGCACC
CGGAGAGGGCGCAGCTGCGGCTGGAGAGTGGCCAATCGGACGCCGGTATG
GCCACAGGGAGACTCGTAGCCGCGCTAGCCTGGCAAGGGTAGCGGGGGGT
GCAAGCATCAATAGCGGAGGCGCCGGAGAAAGTGGCATCTTAACGAATGG
TGGCGACTTTGGCAGAGCAGCGGCTAGGACGGTAGGGCTGGAGGAGGACG
GCATGTTCCGGGCAATCGTTGGGTACTTGTGAAGCTGCCGGTGAGACGAG
AGTCTGCTTGCAACGTATGGTAGAGCTGCGACGTGCCTCGCCGAGCATAG
GGTGTACAGAGCACCCGAGGCAGAGACTTCGAATCCTGCAATGATCGGCG
TGAGGTTTACTCGAGATATGTTGCACCGCGCACCCTGTCCGGCCAGCAGC
CTCCTTCCTGCCGGAGGTTGCCCCGCGTATGCAGCACGAGTAGCTCTCTC
AAACCACCCACCAACGACACCGTCGTACCGTGATCCAGCACAAAGATTCT
TTATGAGCACACCTTGTATGTGCGAGTATGATGGACCTTGTTCCTTTTCC
TTTGTCTGGTCTCCCGCGCGCCTAAAGAGTTCGATACGTTCCAACCTTGC
GTTTCCTTGAACCATGATTACTACCGCTGTAGCACCGCTACCGTTCAGCC
AAGTCGAGTGGCTACTACAGGCCAGTAGTCAGTCGACGATAGTATTTGAG
CAGCACGGGTACGATTCGAACACCTCCGGAATTCTGCACGTGCGGAATAT
TTGTTTGTCCCTCAACCTCGTGAGCACACCCTTGGAGCGACGAGGCAGAA
ATGCCCGCCCTGGCCTGACGTGAGGGTTCTCGTCTGTTTCGCTCGCATGT
CTATAGCCATCAGCAGCAACTCTCTTGTATAACCCTTATTTTGCGCAATC
CAAGAGCAACCAACTATTTTCCAGCTGTCTCAAGGGGTGCAGTCAACACC
ACACGCTTCACACGAATTTGGTTCATACACTGGCCAACCGGTGGTAGCGA
TAGGATAGCTTGCTGTACATTACGAGTTGTCCCCAGCTTTCCGATGCTGT
AGTCTCCCAACCAGGCATCCGGAGTTGATCTTCAGCCGCATTGGTTGATT
TGTCCGTCAGTGCATGTTTACTTTGTTTTTCTTGTTGTCAATTCTTCCGA
CCTGCTTGGCCCTTTCTTGCCCCTACACCAGACTACGGCGGATGCCTGGA
GCCAGATCGTCGGCGTCTGGTCGGTTCTACTACGTAGTTTCGACGGTAGA
CAGCGGCAGGCAGTGGTGATTGGATGATTCGGGCGCGGGGAGCAGAACTG
GCAGAAAGGATACATCGCACGAGACTTTCGTGGCCGGGGCTGCGCCATTG
TGGTTCCCCGTAGGTCTTTTTATCTTGGGCTGTGAACGGAGCTTGATAGC
GGAGGCGACCGACAAGGTGGTAGCGTAAGTTGGTATTTAGATTATTAATA
TTTACGGAGAAGAGCGAAAGTTTTTACGTACGTCGAAGTATGTATCAAGC
CAGGGAATTATGTGGTTTTCGTAGCAGCAAAGAGGCACCGAGTTAGAGAG
GCAGATGTAGCCGAGAGATCAAGAGCGCTGCTGCTGTAGAAATGATAGGC
GTGCGCCGAAGCCGAACACGTCTGAGGTTTTCGTTGTGTAACTGCTGTAC
GCTAGGGAAGGTGGTAAAGGCTGGGGTTTCTGATTGCCAACGAAGTGTTT
GGTGGCCGGGGAGGAATAAGGACGCGGCTATGAACATGTAGGAGAATCGC
TTGAAGAAACTAAGATCATGACCGAGGCCTTTTTTGTTCG
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Coding sequence (CDS) from alignment at S-firma_F_contig1019:16466..19605-

>mRNA_S-firma_F_contig1019.296.1 ID=mRNA_S-firma_F_contig1019.296.1|Name=mRNA_S-firma_F_contig1019.296.1|organism=Sphaerotrichia firma ET2_F female|type=CDS|length=1794bp|location=Sequence derived from alignment at S-firma_F_contig1019:16466..19605- (Sphaerotrichia firma ET2_F female)
ATGCCTCGGGATCAACCCCACTCCATGCTGGTGCCCAGAGGCGGAAAACC
GGAGCTACCAACAGCAGCCCGGGGTGCTAGAGTAAACGCAGCAGACAAGG
AAGGTGGTACGCCGCTGTGCAGGGCAGCCAAGCATGGGCGTCTGGCCGTG
GTGCAAGCTCTCCTGGCTGGGCTGACGAAACCCGACGCTTGCACAGCTTG
GTACGGCCGCTCGAGATTGCTGCCGAACACGACCATGCGGACGTCTTGCG
AGCGTTTTATCGAGAGCGGCTCCGACGTGAACTATGTTGACATCGACGGC
AGGACGGCGCTTCAGAGCGCCGTGCTCTGCCTCGGTTCGGAGGTGATCGA
TGTACTTGTCGAGGCCGCCGGGGCCAGCCTCGAACAAAGGAATGTGATGG
GATGCGCCCCGCTGCACATTGCTGTTAGGAAGGAGGGCCGTCTTGAGGCC
GCGCTCGCCTTGCTGAGGAATGGTGCAGACGTCAACGTGGCGCGAACGGG
GTTCAAAGAAGGAGAAATGCCGTTGCATACGGCAGCACGCAGGGGGTACC
TGGTACCGCAGGGCCGCGGGCGGGCAGGGGGTACCTGGCACCACCGCGGA
GGTGGTAGACCTCCTGCTTCGATGGGGAGCCGACGAAACGATCGTCGACG
AAGAGGGCACAGAGCCGCCGTTGATATTTCGGACGCCGACTTCAACGGCC
CGCGCGACCTGCAGGAGGTTGAGCGTGCGCGGAAGTTCCTGGTCAACGCT
TCTGCTGACAGGGCATGGCGTCGCCGCGGCCACCTGGTCTTGTGCCGTGC
GCACCCGGAGAGGGCGCAGCTGCGGCTGGAGAGTGGCCAATCGGACGCCG
GTATGGCCACAGGGAGACTCGTAGCCGCGCTAGCCTGGCAAGGGTAGATG
CCTCGGGATCAACCCCACTCCATGCTGGTGCCCAGAGGCGGAAAACCGGA
GCTACCAACAGCAGCCCGGGGTGCTAGAGTAAACGCAGCAGACAAGGAAG
GTGGTACGCCGCTGTGCAGGGCAGCCAAGCATGGGCGTCTGGCCGTGGTG
CAAGCTCTCCTGGCTGGGCTGACGAAACCCGACGCTTGCACAGCTTGGTA
CGGCCGCTCGAGATTGCTGCCGAACACGACCATGCGGACGTCTTGCGAGC
GTTTTATCGAGAGCGGCTCCGACGTGAACTATGTTGACATCGACGGCAGG
ACGGCGCTTCAGAGCGCCGTGCTCTGCCTCGGTTCGGAGGTGATCGATGT
ACTTGTCGAGGCCGCCGGGGCCAGCCTCGAACAAAGGAATGTGATGGGAT
GCGCCCCGCTGCACATTGCTGTTAGGAAGGAGGGCCGTCTTGAGGCCGCG
CTCGCCTTGCTGAGGAATGGTGCAGACGTCAACGTGGCGCGAACGGGGTT
CAAAGAAGGAGAAATGCCGTTGCATACGGCAGCACGCAGGGGGTACCTGG
TACCGCAGGGCCGCGGGCGGGCAGGGGGTACCTGGCACCACCGCGGAGGT
GGTAGACCTCCTGCTTCGATGGGGAGCCGACGAAACGATCGTCGACGAAG
AGGGCACAGAGCCGCCGTTGATATTTCGGACGCCGACTTCAACGGCCCGC
GCGACCTGCAGGAGGTTGAGCGTGCGCGGAAGTTCCTGGTCAACGCTTCT
GCTGACAGGGCATGGCGTCGCCGCGGCCACCTGGTCTTGTGCCGTGCGCA
CCCGGAGAGGGCGCAGCTGCGGCTGGAGAGTGGCCAATCGGACGCCGGTA
TGGCCACAGGGAGACTCGTAGCCGCGCTAGCCTGGCAAGGGTAG
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