mRNA_S-firma_F_contig1016.270.1 (mRNA) Sphaerotrichia firma ET2_F female

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1243 bits (3216), Expect = 0.000e+0
Identity = 639/980 (65.20%), Postives = 749/980 (76.43%), Query Frame = 1

Query:  319 STPCPSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHGH--------SNGPGRLSASHGAMMRKDNVEGLSVELAPGTLPANSWEGRAYIKDLLSGLLSHSIDLHATNFWSDPAMAEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANV------FTS-LSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGTTLSCVGGCIFGDEEED-DLVSTEFAQYFDIDRICPAFG---CDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTVRPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEIDTSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAITPATDIGAIERSSSALS 3201
            S P PSL +VENAL TTAK DF G ++AFE+A  WN ERLR    H QD  RAPHLCC +Y HGRE +S L+  LSPEAVR V HSE+ GAC+ A+ASHA A+ ++E  EQ  L+SF+PFPSALK+APGL++H          + G  RLSA HGA MR  NVEGLS+EL PGTL + S E  A+I D L  L+  S+DLH+TNFWSD  M  GE L+ P GA+R RDWSKAA+VVH LSEAA T PGDICSWN V VHHA  D+LLVSGLDHLL+SGRGAGG S+ GE+AELH+ACFMGLVSFLA + EVLRVA R +Q LLNA+ARANIQSAT+TDTPLTDAGLDG+G+V+QVID+G+DE+SCFF HDE GD + HG+Y++E+G N       F+S  SD DD    P   D +     GS    ++ F+GGDFT +PDRRKI+QYINLIKSD    S GD F T+ G + PWLPA+ FEYD +AGHGTH AGS AGATL+ PAE ++C++  T+SCVG C+  DE  D D ++T   +  D+DR+CP F    CD      CLSDDV ETLTNNGGMAQGAKLAIFD FY  FGL   VGNGLWEPCAEA CK+HSNS+GGD+EC LGP+D+ YD +M  NPENLLIFAAGN G+  R +CTMSSPAIAKNVLAVGASTSG T+LS T    P +   G+DTVA FSS GPT D RIKPEV+APGD IYSAASDG  +SHSCRLWAYAGTSMS PIVAGA+AM+RQYF++  F+  D+ AR FC E   C+ FSPSSAT+KAL+INSANLMG S++PDGSRGFGRIHLE GMPL GEGSL LFV+D+  T+IPE TR EYNFDV+A A LD RVTLSW+DPPAT+ S+ QLVHDLDL V+SP+G R+TMW SG+TD+ NVNERVIVDA DVETG WSVW+WA  LTTD QSYSLVVNGAI+P T +GAIERSSS  S
Sbjct:   18 SLPYPSLELVENALSTTAKDDFNGHARAFEAAATWNGERLR----HRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDH---RTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSAT----PGL---GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSS 983          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1134 bits (2932), Expect = 0.000e+0
Identity = 603/957 (63.01%), Postives = 708/957 (73.98%), Query Frame = 1

Query:  331 PSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHGHS--------NGPGRLSASHGAMMRKDNVEGLSVELAPGTLPANSWEGRAYIKDLLSGLLSHSIDLHATNFWSDPAMAEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGTTLSCVGGCIFGDEEEDDLVSTEFAQYF-DIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTVRPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEIDTSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAITPATDIGA 3174
            PSL +VEN L TTAK DF G ++AFE+A  WN ERLR      QD  RAPHLCC +Y HGRE +S L+  LSP+AVR V HSE+HGAC+LATASHA A+ ++E  EQ  L+SF+PFPSALK+APGL++H  S        +G  RL A HG  MR  NVEGL+V+L P TLPA S    ++I DLL  L+S S+DLH+TNFWSDP MA GEHL++  GA+R RDWSKAA+VVH LSEAA T PGDICSW+ V +  AGDD LLV+GLDHLL+SGRGAGG+ EE E+ ELHVACFMGLVSF AGR EVLRV+ RHT+ +LN AAR  IQSA+ TDTPLTDAGLDG+GEVIQV+DTGLDETSCFF+ D+ G+++ HGY+++E    +F   + S    + P                    F  GDFT   +RRK++QYI L+  D +  +    F T +GG  P+LP ++F  DD AGHGTHTAGSAAGATL+DPAET+TC    TL CVGGCI   E  DDLVS        D+DR+CP +GCD   D  CLSD+V ETLT NGGMAQGAKL+IFD F+  FGL+ ++GNG+WEPC EAGCK+HS S GGD  C  G S+I YDDFMYENPENLLIFAAGN G+T R  CTM+SPAI KNVLAVGASTSG T++ +T         +G+D VA FSS G T DGRIKPEVVAPGDM+YSAASDG + SHSCRLWAY GTSMSCPIVAGAAAMVRQYF+D  FY AD+ AR FC +GF C+GFSPS+ATVKALLINSANLMG S+QPD  RGFGRIHLE+GMPL GEG L LFVADA +T+I E T  EY FDVDADAGL+ R TLSWID PATS S+ QLV+DLDL V+SPSG  + MW S +TD  NVNERVIVDA DVETG W+V V++  L TD QSYSLVVNGAI+  T  GA
Sbjct:   32 PSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRS----QDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEE-ESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFI-DDGGEEVDHGYFFEE----LFVGSTGSSQLIYYPV-------------------FEAGDFTYTEERRKVIQYIELVNPDSDYVA----FSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTDP-CLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTE------GGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTD-SHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTGEGA 947          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1103 bits (2853), Expect = 0.000e+0
Identity = 606/1019 (59.47%), Postives = 709/1019 (69.58%), Query Frame = 1

Query:  331 PSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEH----------GHSNGPGRLSASHGAMMRKDNVEGLSVELAPGTLPANSWEGRAYIKDLLSGLLSHSIDLHATNFWSDPAM-AEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGTTLSCVGGCIFGDEEEDDLVS-TEFAQYFDIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTVRPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEIDTSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAITPATDIGAIERSSSALSSDTIDSASPADPGIVVAASGSLAVAPIASSVLVPMVFLVAAFISTTAWTA 3351
            P+L +VENALLTTAK DF G   AFE+A  WN ERLR    H Q+  RAPHL C  Y  G EA S L+  LSPEAVR V HSE+HGAC+ A+ASHA AA ++E  EQ  L++F+PFPS LK+APGL++H            S+G  RLSA HGA MRK NVEGLSVEL PGTL A S E +++I  +L  L+S S+DLH+TNFWSDP M   GEHL++P GA+R RDW KAA+VVH LSEAAST PGDICSWN + VHHAGDD LLVSGLDHLL+SGRGAGG+ EE E+ ELHVACFMGLVSFLAGR EVLRV+ R T+ +LNAAAR  IQSA+ TDTPLTDAGLDG+GE+IQVID+GLDETSC+F+ D+ G+++ HGYY+DE G     S S S D+                    ++  FNGGDF+ YP+RRK                                          AGHGTHTAGSAAGATL+DPAET+TC    TLSCVG CI   +  DDLVS +      D+DR+CP +GCD   D  CLSDDV ETL+NNGGMAQGAKLAIFD FYG +     +GN LWE C EAGCKLHSNS G D+EC +   DI  D FMYEN ENLLIFAAGN G   R +CT+++P I KN LA+GASTSG T++ +T      +  +G+DTVA FSS G T D RIKPEVVAPGD +YSAASDG + +HSCRLWAY GTSMSCPIVAGAAAM+RQYF+D  FY AD+ AR FC +GF C+GFSPS+ATVKALLINSANLMG S++PDG RGFGR+HLE GMPL GEG LVLFVADA +T+I   T  EY FDVDADAGLD R TLSWIDPPAT+ S+ QLVH+LDL V+SP G  + MW S  TD  NVNERVIVDA DV TG W+VWVWA  L TD QSYSLVVNGAI+P T  GA   SS  +SS    ++S    G  V   GSL  AP A  +   +   VAA ++   + A
Sbjct:   27 PALELVENALLTTAKDDFKGHVGAFEAAATWNGERLR----HSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEE-ESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFI-DDSGEEVDHGYYFDELGRAAVYSSSYSSDE--------------------TATIFNGGDFSYYPERRK------------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVIST------VGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTD-THSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGASGSSSFEVSSSATSASSEDGTGAGVT-GGSLVAAPAAGFLAAVVGACVAAMVAAGVFVA 969          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 933 bits (2412), Expect = 0.000e+0
Identity = 531/945 (56.19%), Postives = 636/945 (67.30%), Query Frame = 1

Query:  304 GSSMSSTPCPSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHG-----HSNGPGRLSASHGAMMRKDNVEGLSVELAPGTLPANSWEGRAYIKDLLSGLLSHSIDLHATNFWSDPAMAEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGTTL-SCVGGCIFGDEEE--DDLVST--EFAQY-FDIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTV--RPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEID-TSG---LDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGE-GFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWL-SGMTDTVNVNERVIVDAADVETGVWS 3081
            G S ++ P PSL VVE +LLTTA+  F G +KAFE+A  W  E LR+     +D ARAPHL CT+Y+ G +    LQ+LLSP AVRPV HS  HGAC+  TASHA+A  + +      L S +PFPSALKVAPG+LEHG      S  PGRL+ +HG +MRK NVEGL++EL+PGTLPA+S E   +I +LL  L+S SIDLH  N WSDP  A GEHL  P GA+R R+WS AA +VH L+ A  T PGDICSW+S+ +H+AG+DLLLVSGLDHLL+   GA G+ EE  A EL VACFMGLVSFL+ RLEVLRV+ RH   LLNA AR  IQSATIT+ PLTDAGLDG+G+VIQVIDTGLDETSCFF+ D+ G+++ HG+YY+                              +G +I SS                                      +AR              DD  GHGTH AGSA GAT+  PAET T  +GT +  CVGGCI  D     DDL +   E++ Y  DIDR+CP   CD   D  CLSDDVG+TL ++GGMAQGAKLA FD F         VGNGLWEPC EAGCK+HS S G D+EC +   D+ YDDFMY NPENL++ AAGN GDT   R  CTM SPAI KN LAVGA++SG T+++ T+     ID T+G   +DTVA FSS GPT+DGRIKPEVVAPGD IYSA SDG + +HSCRL A +GTSM+CPIVAGA+AMVRQYF+D  FY AD AAR +  + G +C+ FSPSSATVKALLINSANLMG S++PDG RGFGRIHLE GMPL GEGSL LFVAD++ T++PEL   ++ FDVDA+A LDFR TLSWIDPPA+S S  QLVHDLDL V+SPSG  H MW  SG  D+ NVNERVI+ A DVE+G ++
Sbjct:   23 GGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKE---EDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDS-PYYGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEE--ATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDD-GEEVPHGHYYE-----------------------------ALGEIIPSSS-------------------------------------SAR--------------DDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSD-NHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 921 bits (2381), Expect = 0.000e+0
Identity = 529/968 (54.65%), Postives = 635/968 (65.60%), Query Frame = 1

Query:  304 GSSMSSTPCPSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHG-----HSNGPGRLSASHGAMMRKDNVEGLSVELAPGTLPANSWEGRAYIKDLLSGLLSHSIDLHATNFWSDPAMAEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGTTL-SCVGGCIFGDEEE--DDLVST--EFAQYF-DIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTV--RPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEID-TSG---LDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGE-GFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWL-SGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAI 3150
            G S +S P PSL VVE +LLTTA   F G +K FE+A  W  + +R+     +D ARAPHL CT+Y+ G E  S LQ+LLSPEAVR V HS  HGAC+  TASHA+A  + ++     L S +PFPSALKVAPG+LEHG      S  PGRL+ +HG +MRK NVEGL++EL+PGTLPA+S +   +I +LL  L+S SIDLH  N WSDP  A GEHL  P GA+R R+WS AA +VH L+ A  T PGDICSW+S+ +HHAG+DLLLVSGLDHLL+   GA G+ EE  A EL VACFMGLVSFL+ RLEV RV+ RH   LLNA AR  IQSATIT  PLT AGLDG+G+VIQVI                                                                                   QYINLIK+D     A     T+ G  V W+  + F+ DD   HGTHTAGSA GAT+  PAET T  +GT +  CVGGCI  D     DDL++   E + Y  DIDRICP  GCD E +  CLSD V +TL ++GGMAQGAKL+ FD + G       +GNGLWEPC EAGCK+HS S+G D EC +  +D+ YDDFMY NPENL++ AAGN G+    R  CTM SPAI KN LAVGA++SG T++  T+      D T+G   +DTVA FSS GPT+DGRIKPEVVAPGD +YSA SDG +  HSCRL A +GTSM+CPIVAGA+AMVRQYF+D  FY AD AAR +C + G QC+ FSPSSATVKALLINSANLMG S++PDG RGFGRIHLE GMPL GEGSL LFVAD++ T++PEL+  ++ FDVDA+A LDFR TLSWIDPPA+S S  QLVHDLDL V+SPSG  H MW  SG  D+ NVNERVI+ A +VE+G ++V V    L TD Q+YSLVVNGA+
Sbjct:   23 GGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKE---EDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDN-PYYGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEE--ATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI-----------------------------------------------------------------------------------QYINLIKADSVSGPASST--TSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDV-HSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 873 bits (2256), Expect = 3.190e-304
Identity = 451/696 (64.80%), Postives = 531/696 (76.29%), Query Frame = 1

Query: 1189 MGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANV-FTSLSDSDDQWWLPNEE--------DHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGTTLSCVGGCIFGDEEED-DLVSTEFAQYFDIDRICPAFG---CDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTVRPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEIDTSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAITPATDIGAIERSSSALSSDTIDSASPADP 3237
            MGLVSFLA + EVLRVA R +Q LLNAAARANIQSAT+TDTPLTDAGLDG+G+V+QVID+G+DE+SCFF HDE GD + HG+Y++E+G N   TS S S                  DH+     GS    ++ F+GGDFT YPDRRKI+QYINLIKSD    S GD F T+ G + PWL A+ FEYDD+AGHGTH AGS AGATL+ PAE ++C +  T+SCVG C+  DE  D D ++T + +  D+DR+CP F    CD      CLSDDV ETLTNNGGMAQGAKLAIFD FY TFGL   VG GLWEPCA+A CK+HSNS+GGD+EC LG +D+ YD++M  NPENLLIFAAGN G+  R +CT+ SPAIAKNVLAVGASTSG T+LS T         SG+DTVA FSS GPT D RIKPEV+APGD IYSAASDG  ++HSCRLWAY GTSMS PIVAGA+AM+RQYF++  F+  D+ AR FC E   C+ FSPSSAT+KAL+INSANLMG S++P+GSRGFGRIHLE GMPL GEGSL LFV+D+  T+IPELTR EYNFDV+A AGLD RVTLSW+DPPAT+ S+ QLVHDLDL V+SP+G R+TMW SG+TD+ NVNERVIVDA DVETG WSVW+WA  LTTD QSYSLVVNGAI+P T +GAIERSSS  S+ +   A  A P
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHH----TGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSAT-------PGSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDGLAETAPP 685          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 845 bits (2184), Expect = 1.040e-289
Identity = 486/973 (49.95%), Postives = 624/973 (64.13%), Query Frame = 1

Query:  331 PSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHGHSNGP---------------GRLSASHGAMMRKDNVEGLSVELAPGTLPANSW---EGRAYIKDLLSGLLSHSIDLHATNFWSDPAMAEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGAN--VFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVTCENGT-TLSCVGGCIFGDEEEDDLVSTEFAQYFDIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTV-RPSCTMSSPAIAKNVLAVGASTSGSTQLSTTAL----MSPEIDTSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVE----TGVWSVWVWAKRLTT-DEQSYSLVVNGAITP 3156
            PSL   E  LLTTA +DF   + AF  A AW     R GR+       +P+L C ++  GR AY  L+  L   AV+PV  +  HGAC++ TAS A+AA  L  +E     +F  FP+ALK+APGLL+HG  +GP               GRLS +HGA M+ D+  GL VEL+PGTLP++S    +   +I++LL  L S S+DLHA   WSD  M  G+HL +  GALRGR+WS+AA +V+  SE+    P  +C W  V VHHA +D+LLV+GLDHLLY GR   G SEE EAAEL +AC+MGLVSFLA R EVL+VA  + +  LNAAARA IQSA++  TPLTDAGLDGSGEVIQ+ID G++ET+CF  H + G ++ HGYY+D   ++  +FTS S+S   +                       F GGDF+   DRRK++QYI+LI  D    S+           +P++ A+ F  DD  GHGTH A + AG+T+S PAE V+C +G+  + C GGCI     E    S+E     DI+R+CPAFGC  ++   CL DD  +TL+ +GGMAQGAK+A  D FYG +    + GNGLWE CA+AGCK+HSNS+G D  CQL   D+ YD++MYEN E+LL+F+AGN G T  R +CT+ SP IAKNVL VG+++SG T+LSTT L     +   + S +D V+ FS  G TEDGRIKPEVVAPGDMIYSA+ +G + S  C L AYAGTS S  + +GAAAMVRQYF DS FY AD+ +R FC +GF CD FSP + TVKA+LINSAN+MG   +PD  RGFGRIHLE GMPL G G + LFVAD+++  +    ++++ F+VD DAG++ R TLSW DP A+SLS  QLV+DLDL V +P G R+ MW     DT NVNERVIV A  V     T  W V V  +  T  D  S+SLVV GAI+P
Sbjct:   25 PSLGFFEQTLLTTAGADFDSEA-AFAFASAWQAGD-RQGRS-------SPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQ-LSSWEDFT--TFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEE-EAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGD-GTEVEHGYYFDFVASSNPLFTSSSESRSMF--------------------KAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSG---------MPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCI----TESSTGSSEADYGEDINRLCPAFGCSGDQ---CLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSS--CELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 945          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 791 bits (2042), Expect = 2.230e-272
Identity = 442/743 (59.49%), Postives = 527/743 (70.93%), Query Frame = 1

Query: 1090 LLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFG-ANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSAGDYFDTARGGEVPW-LPAEDFEYDDKAGHG--------THTAGSAAGATLSDPAETVTCENGTTLSCVGGCIFGDEEEDDLVSTEFAQY-FDIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTVRPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEIDTSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAITPATDIGAIERSSSALSSDTIDSASPADPGIVVAASGSLAVAPIA 3285
            +  +GLDHLL+SGRGAGG+ EE E+ ELHVACFMGLVS LAGR EVLRV+ R T+ +LNAAAR  +QSA+ TDTPLTDAGLDG+GE+IQVID+GLDETSC+F+ D+ G+++ HGYY+DE   A V++SL  SD+                     ++  FNGGDF+ YP+RRKI+QYI+L+     P S    F T++G  + + LP + F  DD+A                SAAGATL++PAET+TC    TLSCVG CI   +  DDLVS        D+DR+CP +GCD   D  CLSDDV ETL+NNGGMAQGAKLAIFD FYG +     +GN LWE C EAGCKLHSNS G D+EC +   DI  D FMYEN E+LLIFAAGN G   R +CT+++P I KN LA+GASTSG T++ +T      +  +G++TVA FSS G T D RIKPEVVAPGD +YSAASDG + +HSCRLWAY GTSMSCPIVAGAAAM+RQYF+D  FY AD+ AR FC + F C+GFSPS+ATVKALLINSANLMG S++PDG RGFGR+HLE GMPL GEG+LVLFVADA +T+I E T  EY FDVDADAGLD R TLSWIDPP+T+ S+ QLVH+LDL V+SP G  + MW S  TD  NVNERVIVDA DV TG W+VWVWA  L TD QSYSLVVNGAI+P T  GA   SS  +SS    SAS  D     A  GSL  AP A
Sbjct:    1 MHATGLDHLLFSGRGAGGSHEE-ESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFI-DDSGEEVDHGYYFDELAQAAVYSSLYSSDE---------------------TATIFNGGDFSYYPERRKIIQYIDLVN----PDSPYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVIST------VGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTD-THSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTGEGASGSSSFEVSS-LATSASSEDGTAAGATGGSLVAAPAA 708          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 776 bits (2004), Expect = 8.770e-261
Identity = 495/1084 (45.66%), Postives = 628/1084 (57.93%), Query Frame = 1

Query:  325 PCPSLHVVENALLTTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAY-SLLQDLLSPEAV--RPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHGHSN-----GPGRLSASHGAMMRK---------------------------------------DN-------VEGLSVELAPGTLPANSWEGRA----------YIKDLLSGLLSHSIDLHATNFWS--------DPAMAEG--EHLAAPEGALRGRDWSKAAAVVHGL--SEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKSDQEPQSA--------------------------GDYFDTARGGEVPWLPAEDFEYDDKAGHGTHTAGSAAGATLSDPAETVT------CENGTTLSCVGGCIFGDEE--EDDLVSTEFAQYF-------DIDRICPAFGCDPERDAVCLSDDVGETLTNNGGMAQGAKLAIFDGFYGTFGL-MGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTVRPSCTMSSPAIAKNVLAVGASTSGSTQLSTTALMSPEID----TSGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGEGFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAANTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADV---------------ETGVWSVWVWAKRLTTDE-QSYSLVVNGAITPAT 3162
            P P L V+  ALLTTA   F  + + F++AV W+  RL        D   AP+L C   D  R    + L  +L   A+  R VV S EHG C +ATA+   A ++ +   Q    S+A FPSALK+APGLL+H H +     G GRL  +HG  +R+                                       DN       V GL+VELAPG L A +  G +             +    L+S S+D+H  +FWS        D A AEG  +      G +  R+W++AA V+ GL  +E   T  GD+CSW+ + V H  DDLL + G+DHLL  GRGAGG +E  EA EL VACFM L+SFLA R EVLRV+      LLNAAA  N+QSA++T++P+TDAGLDG+GEVIQ++DTGLD  SCFFV ++ G+ +AHGYY++E G    +  S      W  + + +         +  ++ F GGDF     RRK +       S   P  +                          G+ + T+ G + PW+ A +F  DD  GHGTHTAGSAAGA +S P+ + T      CE G  LSCVGGCI  D+   EDDL++T   QYF       D+DR+CP   CD   +  CL DD  ETL  +GG+A+GA+LAIFD      G+ + + GNG+WEP  EAG KL SNSWG    C     D+ +DDFMY N + LL+FAAGN GD     CTM +PA+AKNVLAVGASTSG  +LS T       D       +D +A FSS GPT D RIKPEVVAPGDMIYSAA  G+  +++CRLW YAGTSMS PIVAGAAA+VRQYF +  FY  D+  R FC + F C+ F+PS+ATVKA+LINSANLMG S +PDG RGFGR+HLE GMPLAGEG   LFVAD+A T+  ELT  EY+F VDA AG+D R TLSWIDPPAT+ SS QLVHDLDL+V SP+G+R++MW  G  D++N NERVIV A+++                 G W+V VW+KRL  +E QSYSLVV GAI P T
Sbjct:   28 PHPPLSVIRGALLTTAGVTFRHQGE-FKAAVEWHEARL-------DDARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQG--QQVFSSWAAFPSALKLAPGLLDHPHDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTI-GDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGG-NEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGS------WFTSWDFN---------VQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWS-GCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDG-NYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC-DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_S-firma_F_contig1016.270.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 650 bits (1676), Expect = 2.000e-213
Identity = 434/1047 (41.45%), Postives = 565/1047 (53.96%), Query Frame = 1

Query:  364 TTAKSDFGGRSKAFESAVAWNRERLRAGRNHHQDPARAPHLCCTDYDHGREAYSLLQDLLSPEAVRPVVHSEEHGACYLATASHADAATVLEHYEQLRLQSFAPFPSALKVAPGLLEHGHSNGP--------------GRLSASHGAMMRKDNVEGLSVELAPGTLPANSWEG--RAYIKDLLSGLLSHSIDLHATNFWSDPAMAEGEHLAAPEGALRGRDWSKAAAVVHGLSEAASTHPGDICSWNSVRVHHAGDDLLLVSGLDHLLYSGRGAGGTSEEGEAAELHVACFMGLVSFLAGRLEVLRVASRHTQGLLNAAARANIQSATITDTPLTDAGLDGSGEVIQVIDTGLDETSCFFVHDEVGDQIAHGYYYDEFGANVFTSLSDSDDQWWLPNEEDHYGSGVVGSVISSSQRFNGGDFTAYPDRRKIVQYINLIKS-DQEPQSAGD----YFDTARGGEVPWLPAEDFEYDDKAG-----------------------------------------------------HGTHTAGSAAGATLSDPA-ETVTCENGTTLSCVGGCIFGDEEEDDLVSTEFAQYFDIDRICPAFGCDPERD---AVCLSDDVGETLTNNGGMAQGAKLAIFDGFY-GTFGLMGIVGNGLWEPCAEAGCKLHSNSWGGDYECQLGPSDIAYDDFMYENPENLLIFAAGNIGDTV----RPSCTMSSPAIAKNVLAVGASTSGS-------------------TQLS---------TTALMSPEIDT--SGLDTVAAFSSLGPTEDGRIKPEVVAPGDMIYSAASDGEEESHSCRLWAYAGTSMSCPIVAGAAAMVRQYFIDSDFYGADLAARSFCGE---GFQCDGFSPSSATVKALLINSANLMGRSTQPDGSRGFGRIHLEKGMPLAGEGSLVLFVADAA--NTTIPELTRVEYNFDVDADAGLDFRVTLSWIDPPATSLSSKQLVHDLDLAVMSPSGVRHTMWLSGMTDTVNVNERVIVDAADVETGVWSVWVWAKRLTTDEQSYSLVVNGAI 3150
            ++A  DF     A+ +A +W  +RL        + + AP++ C DY  GR A + L+   +  AV  V ++E  G C++ TA  + A  V+   E   L S AP   +LK+A  LL+HG    P                L +++G  M  DNV GLSV L+PG L A   E    A+++D  + L S S+++   +FWSD      E+        R R+WS+AAAVV GL+  ++   G+IC +  +++ H  DDL+LV G++HLL   R           +E   AC+MGL+S LA + EVLRV+   +  L NA A A +QSAT T+TPL DAGLDG+GEVIQV+D+GLDETSCFF  D+ G Q+ HG+ +D  G  V               E+D   S V+G+            F     RRKIVQYI L K+ DQ   S+G     Y     G    +  +E   YDD +G                                                     HGT TAGSAAGA  +    E   C       C GGCI   + +  L  + F    D+D  CP + CD   D   + CLSDD  E L  +GG+A GA++++FD  Y GT       GN LW    E G K+H+NSWGG   CQL   +  YD FMYENPE+LLIFAAGN G       R +CT+ SPA+ KN LAVGA++SG                    T  S         T  L +P   T  +G+DTVA FSS GPT DGRIKP+VVAPGD + SA+SDG +  HSC+L A  GTS SCP+VAGAAA+VRQYF D  F+  D+ +R  C E     QC  F+PS+ATVKA++INSA+LMG S++P+G RGFGR+HLE GMP+ G G   L V +AA  +  I EL  V      D DAG++ R TL+W+DPP T+LS+KQL HDLDL V++PSG ++TMW SG+ DTVNV ERVIV    +E+GVWSV V AK L TDEQ YSLVV GAI
Sbjct:   23 SSAFDDFARSQSAYSAASSWYNDRLSYDA---AEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKR---------FPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDD-GLQVEHGFLFD--GVRV---------------EDDGNVSTVLGNY----------SFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTF----DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTD-GHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELVTV------DGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013