prot_S-ischiensis_contig74.18806.1 (polypeptide) Schizocladia ischiensis KU_0333

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A6H5JBI8_9PHAE (Threonine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBI8_9PHAE)

HSP 1 Score: 947 bits (2448), Expect = 0.000e+0
Identity = 453/603 (75.12%), Postives = 521/603 (86.40%), Query Frame = 0

Query:  105 VEEVDLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEETQFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDK--YDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEIQDVLPAQALDVS 705
            VE+ +LPTNESS +LL+IRHTSAHVMAMAVQRLFP TQ TIGPWIDNGFYYDFFNEET  +DSDLK IKKEMDKII+ K+PLV EEVTR+EA+RRIE +GEPFKLEIL+SIKTEPIT+YHVGD+WWDLCAGPHV+TTGDLP K I+LQ++AGAYWRGDE R  LQRIY TAWE+P QLKEHKRRM EAKRRDHRVLGKKL LFSLQEDAGGGLVFWHPKG+TIR LIE+FWK +H+  GYELLYTPH+A+LDLWKTSGHFDFY++GMFDQM+VEG+QYQIKPMNCPFHCLVY+DD+RSYR LPIRWAE+GTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QLEDEII VL+LTEAIL RFGFD+  Y IMLSTRPEKSVG D IW LAT+ L+ AL RKGW++  DEGGGAFYGPKIDLKIRDAIGRTWQCSTVQ DFNLP+RF L+Y+DS G +K PVMLHRAIFGSLERFFGILVENTAG FP WLAP Q+RLLP+ D + D+C + ++    +G+R +VD   ERL K IR AE+ER+PVM+VVGEKE+A+ TLA+RSRG  D+G   ++E  A++  A   A E+ + L  +A + +
Sbjct:   90 VEKEELPTNESSPELLKIRHTSAHVMAMAVQRLFPGTQVTIGPWIDNGFYYDFFNEETTMTDSDLKEIKKEMDKIIRQKLPLVREEVTREEARRRIEALGEPFKLEILDSIKTEPITLYHVGDQWWDLCAGPHVDTTGDLPQKGIELQTLAGAYWRGDENRPALQRIYGTAWENPAQLKEHKRRMVEAKRRDHRVLGKKLDLFSLQEDAGGGLVFWHPKGSTIRMLIENFWKAQHLENGYELLYTPHVANLDLWKTSGHFDFYKDGMFDQMDVEGDQYQIKPMNCPFHCLVYKDDLRSYRHLPIRWAEMGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPEQLEDEIISVLDLTEAILSRFGFDRRVYQIMLSTRPEKSVGTDEIWDLATEGLKAALERKGWDYSVDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQADFNLPDRFKLEYIDSSGERKQPVMLHRAIFGSLERFFGILVENTAGTFPLWLAPEQMRLLPVTDVARDYCLEVQSKFKKAGLRVKVDRGSERLAKQIRTAEKERIPVMSVVGEKEVAAGTLAVRSRGFGDMGDVPVDELIASMLEADAAAKELHEFLEPKAQEAA 692          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A835ZFX3_9STRA (Threonine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZFX3_9STRA)

HSP 1 Score: 941 bits (2433), Expect = 0.000e+0
Identity = 448/593 (75.55%), Postives = 522/593 (88.03%), Query Frame = 0

Query:  105 VEEVDLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEETQFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEIQDVL 697
            V  ++LPTN++S  LL+IRHT+AHVMAMAVQ+LFP+TQ TIGPWID+GFYYDFFN + QFS+ DLKAIKKEMD+IIK+K+PL  EEV+R+EA+RRIE IGE FKLEIL+SIKTEPITIYH+G+EWWDLCAGPHVETTGDLP KAIQLQ+VAGAYWRGDEKREMLQRIY TAWESP+QLKEHKR MEEAKRRDHRVLGKKL L+SLQE+AGGGLVFWHPKGA IRRLIED+WK  H+  GYELLYTPH+A+L+LWKTSGHFDFY+EGMFDQM+VEGEQ+QIKPMNCPFHCLVY+D +RSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLP QLE EII +L+L E +L RFGF KY++MLSTRP KSVG D IW L+T +L+GAL +KGW +  DE GGAFYGPKIDLKI+DAIGR WQCSTVQCDFNLP RF L+Y  +DG+K+ PVMLHRAIFGS+ERFFGILVENTAGDFPFWLAPTQ++LLP+ DA ++F K   A+L  +G+RA+VDVS ERL KMIRNAEQ+R P++ VVGE+E+ +  LA+RSR G D+GV S+++  A L +A+E A+E   V+
Sbjct:   85 VVPIELPTNKNSENLLKIRHTTAHVMAMAVQKLFPETQVTIGPWIDSGFYYDFFNADKQFSEDDLKAIKKEMDRIIKSKLPLRREEVSREEARRRIEAIGEKFKLEILDSIKTEPITIYHIGEEWWDLCAGPHVETTGDLPPKAIQLQNVAGAYWRGDEKREMLQRIYGTAWESPEQLKEHKRLMEEAKRRDHRVLGKKLDLYSLQEEAGGGLVFWHPKGAIIRRLIEDYWKSVHLEEGYELLYTPHVANLNLWKTSGHFDFYREGMFDQMDVEGEQFQIKPMNCPFHCLVYKDAMRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPDQLETEIIAILDLFERVLSRFGFVKYEVMLSTRPAKSVGSDEIWELSTASLKGALEKKGWAYEVDEEGGAFYGPKIDLKIKDAIGRLWQCSTVQCDFNLPNRFGLEYTSTDGTKQQPVMLHRAIFGSVERFFGILVENTAGDFPFWLAPTQVKLLPVTDAQVEFAKATAAVLRKAGIRADVDVSNERLAKMIRNAEQQRTPIICVVGEREVEAGALAVRSRRGGDMGVKSVDDLKALLRQANEEAIEPHQVV 677          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A7S2V1W5_9STRA (Threonine--tRNA ligase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V1W5_9STRA)

HSP 1 Score: 919 bits (2375), Expect = 0.000e+0
Identity = 431/597 (72.19%), Postives = 511/597 (85.59%), Query Frame = 0

Query:  102 KAAVEEVDLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEET-QFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEIQDVL 697
            +A+ + V+LPTNE++  LL+IRHT+AHVMAMAVQ+LFP  Q TIGPWIDNGFYYDF++ +   F+D DLK IKK+MDKIIKA +PL  EEV+R+EA++RIE +GEPFKLEIL+SIKTEPIT+YHVG+EWWDLCAGPHVE+TG LP KAIQL SVAGAYWRGDEKRE+LQRIY TAWE P QLK +K  + EAK+RDHRVLGKKL LFSLQE+AGGGLVFWHP+G+ IRR+IEDFWK+ H  GGYELLYTPH+A+LDLWKTSGHFDFY++GMFDQM+VEG++YQI+PMNCPFHCLVY+D +RSYR+LPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QLEDEI+ V +L E IL +FGF KY+IMLSTRPEKSVGDD IW +AT+AL+GAL RKGW +G DEGGGAFYGPKID+KIRDAIGRTWQCST+QCDFNLPERFDL+YV +D +++ PVM+HRAIFGS+ERF GIL E+TAGDFP WLAPTQLRLLP+ D  LD+C   K      G+R EVD   +RL K IRNAE++R+P+MAVVG+KE+ S  LA+R RGG DLG F L++    +  A E + E+ +VL
Sbjct:   70 EASADIVELPTNENNENLLKIRHTTAHVMAMAVQKLFPNVQVTIGPWIDNGFYYDFYHTDGGAFTDEDLKKIKKQMDKIIKADLPLRREEVSREEAKKRIEALGEPFKLEILDSIKTEPITLYHVGEEWWDLCAGPHVESTGKLPPKAIQLDSVAGAYWRGDEKREVLQRIYGTAWEDPPQLKAYKSMLVEAKKRDHRVLGKKLDLFSLQEEAGGGLVFWHPRGSLIRRMIEDFWKNAHEGGGYELLYTPHVANLDLWKTSGHFDFYRDGMFDQMDVEGDEYQIRPMNCPFHCLVYKDSLRSYRELPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPEQLEDEIVGVCDLVETILSKFGFSKYEIMLSTRPEKSVGDDQIWGMATEALKGALDRKGWSYGIDEGGGAFYGPKIDVKIRDAIGRTWQCSTIQCDFNLPERFDLEYVSNDSTRQRPVMVHRAIFGSIERFMGILTESTAGDFPLWLAPTQLRLLPVSDVHLDYCMSVKQRALKMGIRVEVDRGADRLAKQIRNAEKQRIPIMAVVGDKEVESEELAIRGRGGGDLGTFKLDDILDKIKTACEESKELSEVL 666          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: B5Y5F7_PHATC (Threonine--tRNA ligase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B5Y5F7_PHATC)

HSP 1 Score: 897 bits (2319), Expect = 0.000e+0
Identity = 429/606 (70.79%), Postives = 510/606 (84.16%), Query Frame = 0

Query:  103 AAVEE---VDLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFF-----NEET-------QFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEI 693
            A VEE   V+LPTN++  +LLRIRH+SAHVMAMAVQR+FP+ Q TIGPWID+GFYYDFF     +EE+       + S+ DLK +KK MDKII    P+  E+V+R+EA+RRIE+IGEPFKLEIL+SIKTEPIT+YH+GDEWWDLCAGPHV++TG LP KAIQLQSVAGAYWRGDE REMLQRIYATAW+ PQQLK++K+ +EEAK+RDHR+LGKKL LFS+QEDAGGGLVFWHPKG+ +RR+IEDFWKD HI  GY+++Y+PH+A++DLWKTSGHFDFY+  MFDQM+VE EQYQIKPMNCPFHCL+Y+D++RSYRDLP RWAELGTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QL+DEI+ VL+LTE IL RFGFDKYD+MLSTRPEKSVG D IW  AT ALEGAL+ KGW++  DEGGGAFYGPKIDLKIRDAIGR WQCSTVQCDFNLPERF L+Y  +DG+++ P+M+HRAIFGS+ERFFGIL+EN +GDFP WLAPTQL+LLP+ DA  ++C +  A     G+R EVD   ERL K IRNAEQ R+PVMAVVG KEM S++LA+RSR   DLG F +E+  + L R +  A E+
Sbjct:   63 ATVEEEELVELPTNDNDEELLRIRHSSAHVMAMAVQRIFPEAQVTIGPWIDSGFYYDFFFPERTDEESGETIPARKLSEQDLKDVKKAMDKIIGKDYPISREDVSREEARRRIEKIGEPFKLEILDSIKTEPITLYHIGDEWWDLCAGPHVDSTGKLPKKAIQLQSVAGAYWRGDENREMLQRIYATAWKDPQQLKQYKKMLEEAKKRDHRMLGKKLDLFSIQEDAGGGLVFWHPKGSKVRRMIEDFWKDAHIQDGYDVVYSPHIANIDLWKTSGHFDFYRADMFDQMDVENEQYQIKPMNCPFHCLMYKDELRSYRDLPFRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPEQLQDEIVGVLDLTETILSRFGFDKYDVMLSTRPEKSVGSDEIWDAATVALEGALKVKGWDYNVDEGGGAFYGPKIDLKIRDAIGRQWQCSTVQCDFNLPERFGLEYASADGTRERPIMVHRAIFGSIERFFGILIENCSGDFPLWLAPTQLKLLPVTDAVHEYCNEIAAKALKMGLRIEVDRGSERLAKQIRNAEQARIPVMAVVGMKEMESNSLAVRSRKLGDLGSFEVEDLLSELKRCAAAAEEM 668          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A7S2SSP0_9STRA (Threonine--tRNA ligase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SSP0_9STRA)

HSP 1 Score: 895 bits (2314), Expect = 0.000e+0
Identity = 418/568 (73.59%), Postives = 492/568 (86.62%), Query Frame = 0

Query:  110 LPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFN-EETQFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSL 676
            LPTNE S +LLRIRHTSAHVMAMAVQRLFP+ Q TIGPWIDNGFYYDF N +E  F++SDLKAIKKEMD+IIK K+PL+ EEV+RDEA+ RIE + EPFKLEIL+SI+TEPITIYH+GDEWWDLCAGPHVE TG+LP K IQLQS+AGAYWRGDE R+ML RIY TAWE   QL ++K  +EEAK+RDHRVLGKKL LFS+Q++AGGGLVFWHPKG+T+RR+IEDFWKD H+A GYELLYTPH+A+L+LWKTSGHFDFY+EGMFDQM+VEG++YQIKPMNCPFHCLVY+D +RSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QL DEI+ VL+LTE IL +FGF+ Y++MLSTRPEKSVG D IW  AT AL+  L+RKGWE+  DEGGGAFYGPKID+KIRDAIGRTWQCST+QCDFNLPERFDL+YV S+G ++ P+M+HRAIFGS+ERFFGILVE+TAGD P W AP Q+RLLP+ D +  +C+        +G+R E+D   +RL K IRNAEQ RVP+MAVVG +EM S TL++R+R G D+GV ++
Sbjct:   60 LPTNEDSERLLRIRHTSAHVMAMAVQRLFPEVQVTIGPWIDNGFYYDFKNPDEGGFTESDLKAIKKEMDRIIKKKLPLIREEVSRDEARTRIEALNEPFKLEILDSIETEPITIYHIGDEWWDLCAGPHVEHTGELPPKGIQLQSMAGAYWRGDESRDMLTRIYGTAWEDAAQLSQYKAMLEEAKKRDHRVLGKKLDLFSIQDEAGGGLVFWHPKGSTVRRVIEDFWKDTHLASGYELLYTPHVANLELWKTSGHFDFYREGMFDQMDVEGDEYQIKPMNCPFHCLVYKDSLRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPNQLSDEIVGVLDLTEQILSKFGFNNYEVMLSTRPEKSVGSDEIWDAATAALQDGLQRKGWEYKVDEGGGAFYGPKIDIKIRDAIGRTWQCSTIQCDFNLPERFDLEYVASEGDRQRPIMVHRAIFGSVERFFGILVESTAGDLPLWCAPVQMRLLPVTDEARAYCESIVEKARRAGLRVELDKGADRLAKQIRNAEQSRVPLMAVVGAQEMESGTLSIRARKGGDMGVIAI 627          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A7S1VGN3_9STRA (Threonine--tRNA ligase n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1VGN3_9STRA)

HSP 1 Score: 895 bits (2312), Expect = 0.000e+0
Identity = 417/596 (69.97%), Postives = 502/596 (84.23%), Query Frame = 0

Query:  110 LPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEET------------QFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEI 693
            LPTN++  +LL IRH+SAHVMAMAVQ+++P+ Q TIGPWIDNGFYYDF+  ET            + +D DLK +KK MDKI+    P+  EEVTR+EA+ RIE +GEPFKLEIL+SIKTEPITIYH+G+EWWDLCAGPHVE+TG +P KA+QLQSVAGAYWRGDEKREMLQRIYATAW+ P QLK++K+ ++EAK+RDHR+LG+KL LFS+QEDAGGGLVFWHPKG+T+RR++EDFWK +H+ GGY+L+YTPH+A++DLWKTSGHFDFY+E MFDQM+VE +QYQIKPMNCPFHCL+Y+D++RSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAH++CLP QL++EI+ VL+LTE IL RFGFD+YD+MLSTRPEKSVG D IW  AT AL+GAL  KGW++  DEGGGAFYGPKIDLKIRDAIGR WQCSTVQCDFNLP+RF L+YVD+D  KK P+M+HRAIFGS+ERFFGIL+ENT G+FP W+APTQL+LLP+ D  LDFC +  A     G+R EVD   ERL K IRNAEQ R+P+MAVVG KEM + TLA+RSR   DLG F++ +    L + SE A+E+
Sbjct:   93 LPTNDNDNELLCIRHSSAHVMAMAVQQVYPEAQVTIGPWIDNGFYYDFYFPETTSEETGETIPGRKLTDQDLKKVKKAMDKIVTKNYPITREEVTREEARSRIEALGEPFKLEILDSIKTEPITIYHIGEEWWDLCAGPHVESTGKIPKKALQLQSVAGAYWRGDEKREMLQRIYATAWKDPDQLKQYKKLLQEAKKRDHRMLGQKLNLFSIQEDAGGGLVFWHPKGSTVRRIVEDFWKQKHLDGGYDLVYTPHIANIDLWKTSGHFDFYKEDMFDQMQVENDQYQIKPMNCPFHCLMYKDELRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIYCLPSQLQEEIVAVLDLTEGILSRFGFDQYDVMLSTRPEKSVGGDEIWDAATVALKGALVAKGWDYSVDEGGGAFYGPKIDLKIRDAIGRQWQCSTVQCDFNLPDRFGLEYVDADTKKKQPIMVHRAIFGSVERFFGILIENTEGNFPLWMAPTQLKLLPVTDVVLDFCHEVAAEAKKKGIRIEVDRGSERLAKQIRNAEQARIPIMAVVGMKEMETKTLAVRSRKLGDLGSFAISDLIDELVQCSEAAVEM 688          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A1Z5K8X1_FISSO (Threonine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K8X1_FISSO)

HSP 1 Score: 894 bits (2311), Expect = 0.000e+0
Identity = 421/602 (69.93%), Postives = 506/602 (84.05%), Query Frame = 0

Query:  104 AVEEVDLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEET------------QFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEI 693
            A E + LPTNE   +LL+IRH+SAHVMAMAVQ++FP+ Q TIGPWIDNGFYYDF+  +T            + ++ DLKA+KKEMDKII    P+  EEV+R+EA+RRI EIGEPFKLEIL+SIKTEPITIYH+G+EWWDLCAGPHVE+T  +P KAI L SVAGAYWRGDEKREMLQRIYATAW+ PQQLK++K+ +EEAK+RDHR+LGKKL LFS+QE+AGGGLVFWHPKG+ IRR+IEDFWK+ H   GY+++Y+PH+A+++LWKTSGHFDFY+  MFDQM+VE EQYQIKPMNCPFHCL+++D++RSYRDLP RWAELGTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QLEDEI+ VL+LTE IL RFGFDKYDIMLST+PEK+VG D IW  AT ALEGAL++KGWE+G DEGGGAFYGPKIDLKIRDAIGR WQCSTVQCDFNLP+RF L+YV ++G+++ P+M+HRAIFGS+ERFFGIL+EN +GDFP WLAPTQ +LLP+ DA  D+C +  A     G+R EVD   ERL K IRNAEQ+RVP+MAVVG KEM ++ LA+RSR   DLG F++++  + LSR S  A E+
Sbjct:   61 AEEIIQLPTNEDDEELLKIRHSSAHVMAMAVQQVFPEAQVTIGPWIDNGFYYDFYFPDTVDKETGEKVPSRKLTEQDLKAVKKEMDKIISKDYPIRREEVSREEARRRINEIGEPFKLEILDSIKTEPITIYHIGEEWWDLCAGPHVESTSSIPKKAISLDSVAGAYWRGDEKREMLQRIYATAWKDPQQLKQYKKMLEEAKKRDHRMLGKKLDLFSVQEEAGGGLVFWHPKGSKIRRMIEDFWKNAHEDAGYDIVYSPHIANINLWKTSGHFDFYRSDMFDQMDVENEQYQIKPMNCPFHCLMFKDELRSYRDLPFRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPEQLEDEIVGVLDLTERILSRFGFDKYDIMLSTKPEKAVGSDDIWEAATKALEGALKKKGWEYGVDEGGGAFYGPKIDLKIRDAIGRQWQCSTVQCDFNLPDRFGLEYVSAEGTREQPIMVHRAIFGSMERFFGILIENCSGDFPLWLAPTQFKLLPVTDAVHDYCNEVAAKAKKMGIRVEVDRGSERLAKQIRNAEQQRVPIMAVVGVKEMETNNLAVRSRKLGDLGSFNVDDLLSELSRCSAAAEEM 662          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A4D9D5P2_9STRA (Threonine--tRNA ligase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D5P2_9STRA)

HSP 1 Score: 892 bits (2305), Expect = 0.000e+0
Identity = 417/592 (70.44%), Postives = 500/592 (84.46%), Query Frame = 0

Query:  106 EEVDLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEETQFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEIQDVL 697
            + V LP+N+ S  LLRIRHTSAHVMAMAVQRLFP  Q TIGPWI+NGFYYDF   +T+F++ DL  IKKEM++IIK ++PL  EEVTR+EA++RIE + EP+KLEIL+ IKTEPITIYH+GDEWWDLCAGPHVE+TGDLP+ AI+L+S+AGAYWRGDEKR ML RIY TAWES +QLKE++R MEEAK+RDHRVLG+KL LFS+QEDAGGGLVFWHPKGAT+R+++EDFWKDEH    Y+LLYTPH+A+L LWKTSGHFDFY+EGMFDQMEVE E +QI+PMNCPFHCL+++D +RSYR+LPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QL+DEI  VL+L E+IL +FGF  Y++MLSTRPEKSVG + IW  AT+AL GAL RKGW +  DEGGGAFYGPKID+KIRDAIGR WQCST+QCDFNLPERFDL+YV  DGSKK P+M+HRAIFGS+ERFFGILVENTAGDFP W++P Q+RLLP+ D ++D  +  K  L+ +G R EVD   ERLGK IRNAEQ R+PVM ++G KE+ S  + +R+R G  LG++ +EE A+ L  A E   E+ +VL
Sbjct:  121 QAVKLPSNDQSPNLLRIRHTSAHVMAMAVQRLFPSVQVTIGPWIENGFYYDFDTGDTKFTEEDLSKIKKEMERIIKKRLPLRREEVTREEARKRIEAMQEPYKLEILDGIKTEPITIYHIGDEWWDLCAGPHVESTGDLPSNAIKLESLAGAYWRGDEKRAMLTRIYGTAWESKEQLKEYQRLMEEAKKRDHRVLGRKLDLFSIQEDAGGGLVFWHPKGATVRKIVEDFWKDEHERREYQLLYTPHMANLQLWKTSGHFDFYKEGMFDQMEVENEAFQIRPMNCPFHCLIFKDTLRSYRELPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPSQLQDEIRGVLDLVESILSKFGFVDYEVMLSTRPEKSVGSEEIWQAATEALVGALERKGWGYEVDEGGGAFYGPKIDIKIRDAIGRLWQCSTIQCDFNLPERFDLEYVADDGSKKRPIMVHRAIFGSIERFFGILVENTAGDFPLWMSPVQMRLLPVTDKAMDHAQDIKKRLSSAGFRVEVDPGSERLGKQIRNAEQARIPVMGIIGAKEVESGGVTVRTRLGGQLGLYGVEELASGLVSAVESTKELHEVL 712          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A7S0D919_9EUKA (Threonine--tRNA ligase n=2 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0D919_9EUKA)

HSP 1 Score: 885 bits (2286), Expect = 0.000e+0
Identity = 417/568 (73.42%), Postives = 484/568 (85.21%), Query Frame = 0

Query:  130 MAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEETQFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEIQDVL 697
            MAMAVQRL+P  Q TIGPW+DNGFYYDF+N E  F + DLK IKKEM+KIIK+ +PL  EEV+R+EA+RRIE I EP+KLEIL++IKTEPITIYH+GDEWWDLCAGPHVETTG LP  AI+LQ++AGAYWRGDEKREMLQRIY TAWE+  QLK +K  + EAKRRDHRVLG KL LFS+QEDAGGGLVFWHPKG+TIR+LIEDFWKD HI  GYELLYTPH+A+LDLWKTSGHFDFYQEGMFDQM+VEG+ YQIKPMNCPFHCLVY+D +RSYR+LPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAH+FCLP QLEDE++ VL+LTE+IL RFGFD Y+I LSTRPEKSVG D IW  A  AL GAL+RKGW+F  DEGGGAFYGPKIDLKI+DAIGRTWQCST+QCDFNLPERFDL+YV +D S++ P+M+HRAIFGS+ERFFGILVENTAGDFP WLAP QL+LLP+ +    FC Q K   A +G+R  VD S ERLGK IRNAE E++PV+AVVG+KE+ S++LA+R+R   DLG F ++   + LS A    +E+  V+
Sbjct:    1 MAMAVQRLYPGVQVTIGPWVDNGFYYDFYNPENPFQEEDLKKIKKEMEKIIKSDLPLRREEVSREEAKRRIEAINEPYKLEILDAIKTEPITIYHIGDEWWDLCAGPHVETTGKLPKNAIELQTIAGAYWRGDEKREMLQRIYGTAWENKSQLKAYKDLLAEAKRRDHRVLGTKLDLFSIQEDAGGGLVFWHPKGSTIRKLIEDFWKDLHIQNGYELLYTPHVANLDLWKTSGHFDFYQEGMFDQMDVEGDMYQIKPMNCPFHCLVYKDKLRSYRELPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIFCLPNQLEDELVGVLDLTESILSRFGFDNYEINLSTRPEKSVGSDDIWDKAETALRGALKRKGWDFAVDEGGGAFYGPKIDLKIKDAIGRTWQCSTIQCDFNLPERFDLEYVSADSSRQRPIMVHRAIFGSIERFFGILVENTAGDFPVWLAPVQLKLLPVTEEFRPFCNQVKEQAAKAGIRVVVDQSAERLGKQIRNAEMEKIPVVAVVGQKEVDSNSLAIRTRRAGDLGNFDVDNIISTLSDAIHEGVELDAVM 568          

BLAST of mRNA_S-ischiensis_contig74.18806.1 vs. uniprot
Match: A0A1E7EYI6_9STRA (Threonine--tRNA ligase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EYI6_9STRA)

HSP 1 Score: 880 bits (2275), Expect = 2.410e-315
Identity = 415/597 (69.51%), Postives = 495/597 (82.91%), Query Frame = 0

Query:  109 DLPTNESSGKLLRIRHTSAHVMAMAVQRLFPKTQCTIGPWIDNGFYYDFFNEET------------QFSDSDLKAIKKEMDKIIKAKMPLVVEEVTRDEAQRRIEEIGEPFKLEILESIKTEPITIYHVGDEWWDLCAGPHVETTGDLPAKAIQLQSVAGAYWRGDEKREMLQRIYATAWESPQQLKEHKRRMEEAKRRDHRVLGKKLGLFSLQEDAGGGLVFWHPKGATIRRLIEDFWKDEHIAGGYELLYTPHLASLDLWKTSGHFDFYQEGMFDQMEVEGEQYQIKPMNCPFHCLVYRDDIRSYRDLPIRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHVFCLPMQLEDEIIKVLNLTEAILKRFGFDKYDIMLSTRPEKSVGDDAIWSLATDALEGALRRKGWEFGTDEGGGAFYGPKIDLKIRDAIGRTWQCSTVQCDFNLPERFDLKYVDSDGSKKVPVMLHRAIFGSLERFFGILVENTAGDFPFWLAPTQLRLLPIQDASLDFCKQAKAMLAVSGVRAEVDVSGERLGKMIRNAEQERVPVMAVVGEKEMASSTLALRSRGGKDLGVFSLEEAAAALSRASEGALEI 693
            +LPTNE+ G+LLRIRH+S HVMAMAVQ+LFP+ Q TIGPWIDNGFYYDF+  ET            + S  DLK +KK+MDKII    P+  EE+TR+EA+RRI+EI EPFKLEILE +  EPITIYH+GDEWWDLCAGPHVE+T  +P KAI LQSVAGAYWRGDE REMLQRIYAT W+ P QLK +K+ +EEAK+RDHR+LGKKL LFS+QE AGGGLVFWHPKG+ +R++IED+WKD H   GY+++YTPH+A+LDLWKTSGHFDFY++ MFDQMEVE EQYQIKPMNCPFHCL+Y++++RSYRDLPIRW ELGTVYRYERSGTLHGL RVRGFTQDDAH+FCLP QLEDEI++VL+LTE+IL +FGFDKYD+MLSTRPEK+VG D IW  AT ALEGAL RKGW +  DEGGGAFYGPKIDLKIRDAIGR WQCST+QCDFNLPERF+L+Y  +D +K+ P+M+HRAIFGSLERFFGIL+EN  G+FP WLAP Q+RLLP+ DA+ D+C +       +G+R EVD   ERL K IRNAEQ+RVP+MAVVGEKEM S TLA+RSR   DLG F++++  A L + +E  +E+
Sbjct:   20 ELPTNENDGELLRIRHSSGHVMAMAVQKLFPEAQTTIGPWIDNGFYYDFYFPETIDAETGETIESRKLSTDDLKKVKKQMDKIISKNYPITREEITREEAERRIKEINEPFKLEILEGL-VEPITIYHIGDEWWDLCAGPHVESTNQIPKKAIALQSVAGAYWRGDENREMLQRIYATGWKDPMQLKRYKKMLEEAKKRDHRMLGKKLDLFSIQEGAGGGLVFWHPKGSKVRKIIEDYWKDAHAKDGYDVIYTPHIANLDLWKTSGHFDFYKDDMFDQMEVENEQYQIKPMNCPFHCLMYKNELRSYRDLPIRWGELGTVYRYERSGTLHGLFRVRGFTQDDAHIFCLPTQLEDEIVQVLDLTESILTKFGFDKYDVMLSTRPEKAVGSDDIWEAATKALEGALVRKGWPYKIDEGGGAFYGPKIDLKIRDAIGRQWQCSTIQCDFNLPERFELEYKTADNTKERPIMVHRAIFGSLERFFGILIENCEGNFPLWLAPEQMRLLPVTDAAQDYCYEVAKKAKKAGLRIEVDRGNERLPKQIRNAEQQRVPIMAVVGEKEMESGTLAVRSRKLGDLGSFTVDDLLAELHQCTEDTVEM 615