prot_S-ischiensis_contig97.21133.1 (polypeptide) Schizocladia ischiensis KU_0333

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A6H5L2M1_9PHAE (Isoleucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L2M1_9PHAE)

HSP 1 Score: 1481 bits (3834), Expect = 0.000e+0
Identity = 720/980 (73.47%), Postives = 824/980 (84.08%), Query Frame = 0

Query:  113 DGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKAVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETGEPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQA---FVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHTDHPTICSRCHGVVQEAGVA 1089
            +  Y+ TVILPQT F+QRANAVKREPELQ FW+EERIYE+ ++ N G KFVLHDGPPYANGDLHIGHALNKILKDFINRYQ+LRGRK  YVPGWDCHGLPIELKVLQSMKSKER G+TP+ LR+KAA FA+ TV+KQS SF+RYGVWGD+ +PYLTLQPEYEAAQ+EVFGKMF EGHI+RGRKPVNWSPSSRTALAEAELEYP  H STSVYAAF+V E + AL  + +   GGL VSVWTTTPWTLPANLAVAV+E ++YSVV H  LGDR+  L+VA DL   V  K+GLEEG+ ++ + TF G D+ GTKYQHP+C RVSEVVIGGDYITTE GTGLVHTAPGHGQEDY+TGQKYGLPLLSPVDDAG+FT EAGDRFKGL+VLKEGNEEV+VA+EEAG LL +EAY HKYPYDWRTKKPTIFRAT QWFASVDRFRD AL AI++V WIPEVG++RI++MV GRSDWCISRQR+WG+PIPV Y+ E+GEP+LT ESIEH+RA+VAE G+D+WWE++ A+LLPPS +  A+QW+RGTDTMDVWFDSGSSWAGVA  R  + YP DLYLEGSDQHRGWFQSSLLTSVA+ G APYKTVLTHGFVLDEKG+KMSKSLGNTLDP EVIEGGNNKKQKPAYGADVLRLWVS+VDY+GDVCVG  IIKQ S+SYRKLRNT RYLTG+LFDF+P +D+VPYEDLPSLDKYML  LS V++EVE AF+ YQFY+ASQALQRFA  DLSNFYLD AKDRLYISS DD RRRSCQTVL  MLM LS  MAPILPHMAEDVWQTLPF P GGH+SIF +GW        EA   EKW ++R LR+DVNK LEQAR  K +G+SLE QA   F++C D  L+A LE + GD+ L   P  +NGVDDLRFVLMVS++ L S+      +     T+ +++S SGC V V+ A G+KCERCWY C+SVG+H DHP++CSRCHGVV+  G+A
Sbjct:  105 ESYYKKTVILPQTGFEQRANAVKREPELQAFWDEERIYERVLDDNTGEKFVLHDGPPYANGDLHIGHALNKILKDFINRYQMLRGRKVGYVPGWDCHGLPIELKVLQSMKSKERRGMTPVQLRKKAAEFARNTVSKQSASFRRYGVWGDFEKPYLTLQPEYEAAQIEVFGKMFTEGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVKEATPALEKY-MGSGGGLAVSVWTTTPWTLPANLAVAVSETIQYSVVEHASLGDRK--LLVATDLVGAVSAKIGLEEGDTLKVVETFTGKDLIGTKYQHPLCDRVSEVVIGGDYITTETGTGLVHTAPGHGQEDYVTGQKYGLPLLSPVDDAGKFTAEAGDRFKGLNVLKEGNEEVLVALEEAGGLLAREAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDTALSAIEKVQWIPEVGQRRITTMVEGRSDWCISRQRSWGLPIPVFYNIESGEPMLTTESIEHIRAIVAEHGSDSWWEMEVADLLPPSLQGEADQWRRGTDTMDVWFDSGSSWAGVAKAREELDYPADLYLEGSDQHRGWFQSSLLTSVAANGHAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPTEVIEGGNNKKQKPAYGADVLRLWVSSVDYSGDVCVGDQIIKQASESYRKLRNTLRYLTGSLFDFDPEKDSVPYEDLPSLDKYMLGMLSSVLEEVENAFDGYQFYKASQALQRFATTDLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSTVMAPILPHMAEDVWQTLPFKPEGGHRSIFQAGWATERHAQHEA---EKWGRLRTLRNDVNKALEQARGAKVLGSSLEGQASLVFIYCSDEGLKAELESLLGDETLKREPELSNGVDDLRFVLMVSKINLVSSAEAVDSACAEGMTVAAKDSESGCVVGVSRAPGNKCERCWYHCDSVGSHDDHPSLCSRCHGVVEGLGLA 1078          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A835YNF4_9STRA (Isoleucine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNF4_9STRA)

HSP 1 Score: 1359 bits (3517), Expect = 0.000e+0
Identity = 685/983 (69.68%), Postives = 782/983 (79.55%), Query Frame = 0

Query:  110 KKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKAVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETGEPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGD-QRLLPSPLSTNGVDDLRFVLMVSQVRLYSA--FVQSGVSAETTFTLGSEESVSGCTVAVAPAKGD--KCERCWYQCESVGTHTDHPTICSRCHGVVQEAG 1087
            KK D  Y+++VILPQT F QRANAV REPELQ++W E R+YE+  E N G  +VLHDGPPYANGDLHIGHALNKILKDFINRYQ+L+G++A +VPGWDCHGLPIELKVLQSMKS ERAGLT L LR +AAAFA  TV +Q ++F+RYGVW  W  PYLTLQPEYEAAQ+EVFGKM   GHIYRGRKPVNWSPSSRTALAEAELEYP DH+STS+YAAF+V +PSAALA  A A    + V++WTTTPWT+PANLAVAVNE+L+Y +V H  LGD+Q  LVVAK+L   +  K+G  EGEQ E +AT  G D+AGT YQHP+  R S+VVIGGDYITTE GTGLVHTAPGHGQEDY TG KYGLPLLSPVDDAG FTEEAG+RF+GLSVLKEGNE V+ AM E+GALL  EAY HKYPYDWRTKKPTIFRAT Q  A+    R +  +    V WIPEVGK RI+ MV GRSDWCISRQR+WGVPIPV YHKETGEPLLT ESI HV  +V EKGTDAW+ +  AELLP S    AE W +GTDTMDVWFDSGSSWAGVA  R G+ YP D+YLEGSDQHRGWFQSSLLTSVA+ G AP+KTVLTHGFVLDEKGFKMSKSLGNT+DP+EVIEGG N+K KPA+GADVLRLWVS VDY+GDV VG NI+KQV +SYRKLRNT RYLTGNLFDF+P  D + Y+DLPSLDK++L RLS VM+EVEEA+E+YQFYRASQA+QRFAV DLSNFYLDTAKDRLYIS P   RRRSCQTVL  +L++LSKAMAPILPHMAED+WQTLPF  P   KSIF +GWV A  P+ EA+    W ++RLLR DVNK LE AR+ K +GASLEA  F+HC D+ L ATL  M+GD   +   P+STNGVDDLRFVLMVSQV L ++   V +  +A    T   E   SGC V+VA A G   KC+RCWYQC +VG    HP +C+RC  V+   G
Sbjct:   33 KKPDSPYKTSVILPQTSFNQRANAVVREPELQQYWAENRVYEQLAESNPGDVYVLHDGPPYANGDLHIGHALNKILKDFINRYQLLQGKRARFVPGWDCHGLPIELKVLQSMKSGERAGLTTLQLRERAAAFATATVERQRDAFKRYGVWALWDEPYLTLQPEYEAAQIEVFGKMLDAGHIYRGRKPVNWSPSSRTALAEAELEYPPDHISTSMYAAFKVVQPSAALAVHAAA-GADVRVAIWTTTPWTIPANLAVAVNEKLDYCLVTHAVLGDKQ--LVVAKELIPALSAKLGAPEGEQFEVVATLSGADLAGTTYQHPLYERQSQVVIGGDYITTETGTGLVHTAPGHGQEDYQTGLKYGLPLLSPVDDAGCFTEEAGERFQGLSVLKEGNEAVLEAMRESGALLLSEAYNHKYPYDWRTKKPTIFRATDQVSAAAAAARASCCRCA--VQWIPEVGKNRITGMVQGRSDWCISRQRSWGVPIPVFYHKETGEPLLTQESIAHVVDIVREKGTDAWFRMSVAELLPLSHAADAEHWDKGTDTMDVWFDSGSSWAGVANGREGLRYPADMYLEGSDQHRGWFQSSLLTSVAANGHAPFKTVLTHGFVLDEKGFKMSKSLGNTVDPKEVIEGGANQKLKPAWGADVLRLWVSTVDYSGDVPVGDNILKQVGESYRKLRNTARYLTGNLFDFDPAADAIAYDDLPSLDKWVLGRLSDVMREVEEAYETYQFYRASQAIQRFAVQDLSNFYLDTAKDRLYISEPGAARRRSCQTVLHHVLINLSKAMAPILPHMAEDIWQTLPF--PVAEKSIFQTGWVDASFPSHEAAV---WDRLRLLRADVNKALEAARTAKLLGASLEAAVFIHCEDSELLATLTAMKGDADTITQHPVSTNGVDDLRFVLMVSQVELLASRDAVAAAAAAPELHTAAGE---SGCAVSVARAGGGRTKCDRCWYQCSTVGASHAHPGVCARCASVLAATG 1002          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A7S1ZYZ0_9STRA (Isoleucine--tRNA ligase n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZYZ0_9STRA)

HSP 1 Score: 1285 bits (3325), Expect = 0.000e+0
Identity = 643/988 (65.08%), Postives = 772/988 (78.14%), Query Frame = 0

Query:  108 GKKKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEK---AVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETG-EPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNP-GEDT---VPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLY-SAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGT-HTDHPTICSRC-HGVVQ 1084
            GKKK+  VY  T++LP+T F QRANAV REPELQ++W++  +Y K     E  +  +FVLHDGPPYANGDLHIGHALNK+LKDFINR Q+L+G++ HYVPGWDCHGLPIELKVLQ+MKSKER  LTP+ LR KAA+FAKETVAKQSESFQRYGV+GD+  PYLTLQP++EA Q++VFG+M+++G+I+RGRKPV+WSPSSRTALAEAELEYP+ H+S S+Y AF V  PS AL  F  A DGGL V++WTTTPWT+PANLA+AVN +LEY+VV H K G     L+VA DL  ++  K  L EGE++  LAT KG +I GT Y HP+  R S V+ GGDYITTE+GTGLVHTAPGHGQEDYLTG K GL LLSPVDD GRFT EAG+RF G++VLKEGNEEV+ A++E G+LL+ E Y HKYPYDWRTKKPTIFRAT QWFASV+ FRD AL AID V W+PEVGK RI+S V+GR DWCISRQR+WGVPIPV Y +ETG E LL   +++HV+ + +E G+DAWW++D  +LLP   +E AE+W++GTDTMDVWFDSGSSWAGVA  R  + YP DLYLEGSDQHRGWFQSSLLTSVA+ G+APYKTVLTHGFVLDEKG+KMSKSLGN +DP +VIEGGNNKKQ+PAYGADVLRLWV+ VDY+GDVC+G NIIKQ  ++YRKLRNT RYL GNL D+ P G+D    VPYEDLPS+DK+ML RLS +++EV+EA + YQF RA+Q + RFA ADLSNFYLD AKDRLYISS DDPRRRSCQTVL A L   SKA+APILPHMAED+WQ LP+  P   +S+F  GW G    +    +EE+W  VR LRDDVNK+LE AR+ K +GASL+A A+V+ PD S R  L  +  D+ L+  P+ TNG+DDLR  LM+SQV+L  SA + S    E+  + G+    SGCTV V  A+G KC RCW+  E VG     H  +C RC H V Q
Sbjct:   65 GKKKEKNVYAPTILLPETPFSQRANAVVREPELQQYWKDSDLYNKLSSVAEEAKAPRFVLHDGPPYANGDLHIGHALNKLLKDFINRKQLLKGKQVHYVPGWDCHGLPIELKVLQTMKSKERQALTPITLREKAASFAKETVAKQSESFQRYGVFGDFENPYLTLQPQFEAEQIKVFGEMYKKGYIFRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYVAFDVDTPSDALKEFHDA-DGGLRVAIWTTTPWTMPANLAIAVNPDLEYAVVEHEKTG----RLLVAADLAESLATKFELPEGEKLSILATMKGSEIIGTTYSHPLYDRKSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKAGLDLLSPVDDLGRFTIEAGERFAGMNVLKEGNEEVVTALQETGSLLRAEDYGHKYPYDWRTKKPTIFRATDQWFASVEGFRDAALDAIDTVRWVPEVGKNRITSFVSGRGDWCISRQRSWGVPIPVFYDEETGNEVLLDEHTLDHVQKIFSEHGSDAWWKMDEKDLLPEMYKEEAEKWKKGTDTMDVWFDSGSSWAGVASARDELQYPADLYLEGSDQHRGWFQSSLLTSVAAQGQAPYKTVLTHGFVLDEKGYKMSKSLGNVIDPLKVIEGGNNKKQEPAYGADVLRLWVATVDYSGDVCIGNNIIKQTFETYRKLRNTARYLIGNLNDYVPEGQDNSNAVPYEDLPSMDKWMLGRLSALLREVDEATDDYQFSRATQEVLRFATADLSNFYLDVAKDRLYISSIDDPRRRSCQTVLHACLEGFSKAIAPILPHMAEDIWQNLPYKKP--TESVFEGGWPGHL-MSFPEHDEEQWTVVRNLRDDVNKVLESARADKLVGASLDAAAYVYAPDESTRDILAKLESDEHLVYPPVKTNGIDDLRTALMISQVKLVDSADLVSEACDESYVSKGA---FSGCTVGVKKAEGKKCGRCWFYDEQVGNLGLPHDDLCQRCGHAVFQ 1041          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A7S4II32_9STRA (Isoleucine--tRNA ligase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4II32_9STRA)

HSP 1 Score: 1280 bits (3312), Expect = 0.000e+0
Identity = 633/978 (64.72%), Postives = 752/978 (76.89%), Query Frame = 0

Query:  115 VYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEK---AVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPF-AVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETG-EPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNP----GEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHTDH----PTICSRC 1079
            VY  T++LP+T F QRANA KREPELQ+FW+   +Y +   + E     +FVLHDGPPYANGDLHIGHALNK+LKDFINR+QIL+G++ HYVPGWDCHGLPIELKVLQS+KSKER GLTP+ LR KAA FAK TV KQSESFQRYGV GD+ +PYLTLQP++EAAQ+ VFG+MFR G+I+RGRKPV+WSPSSRTALAEAELEYP+ HVS S+Y AF+V EPS  L P+     D  L V+VWTTTPWT+PANLAVAVN ELEYSVV H + G     L+VA DL  ++  K  L EGE    LAT +G D+ GT Y HP+ PR S V+ GGDYITTE+GTGLVHTAPGHGQEDYLTG K GL LLSPVDDAGRFT+EAG++F+G++VLKEGN+ ++ A+EE  +LL  E Y HKYPYDWRTKKPTIFRAT QWFASV+ FRD AL+A+D V WIP+VGK RI+S V GR DWCISRQR+WGVPIPV Y +ETG E LL  +++EH+++L +E G+D WW +D  ELLP   R  A +W++GTDTMDVWFDSGSSWAGVA  R  + YP DLYLEGSDQHRGWFQSSLLTSVA+ GKAPYK VLTHGFVLDEKGFKMSKSLGN +DP +VIEGGNNKKQ+PAYGADVLRLWV++VDY GDVC+G NIIKQ  +SYRKLRNT RYL GNL D+ P    G   VPY+DLPS+DK+ML RLS V++EV+EA + YQF RA+Q + RFA ADLSNFYLD AKDRLYIS  DD RRRSCQTVL A L   +KA+AP+LPHMAED+WQ +P+   G   S+F  G+  AG       + E+W  VR LRDDVNK LE AR+ K +GASL+A A+VHCPD  +R  L+G+ GD+ L+  P+ +NGVDDLR  LM+SQV L  +        +  + +  E+  SGCTV V  A G KC RCW+  E+VGT TD     P +C RC
Sbjct:   33 VYAPTILLPETSFSQRANAAKREPELQQFWKSTDLYGRLSSSAEEAGADRFVLHDGPPYANGDLHIGHALNKLLKDFINRHQILKGKQVHYVPGWDCHGLPIELKVLQSLKSKERQGLTPITLREKAADFAKATVEKQSESFQRYGVIGDFDKPYLTLQPQFEAAQIGVFGEMFRRGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSIYVAFEVEEPSDKLRPYHGERSDDRLKVAVWTTTPWTMPANLAVAVNPELEYSVVEHERTG----RLLVATDLANSLASKFSLPEGEAFAVLATMRGEDVVGTSYNHPLYPRTSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLDLLSPVDDAGRFTKEAGEKFEGMNVLKEGNDAIVEALEETKSLLLAEDYGHKYPYDWRTKKPTIFRATDQWFASVEGFRDEALEAVDTVKWIPDVGKNRITSFVVGRGDWCISRQRSWGVPIPVFYDRETGNEVLLDEKTLEHIQSLFSEHGSDCWWTMDEEELLPEEYRADASKWKKGTDTMDVWFDSGSSWAGVARSRDELAYPADLYLEGSDQHRGWFQSSLLTSVAAEGKAPYKAVLTHGFVLDEKGFKMSKSLGNVVDPLKVIEGGNNKKQEPAYGADVLRLWVASVDYTGDVCIGNNIIKQTFESYRKLRNTARYLIGNLADYVPEGRDGSTAVPYDDLPSMDKWMLGRLSSVLREVDEAMDDYQFSRATQEILRFASADLSNFYLDVAKDRLYISGVDDFRRRSCQTVLAACLEGFAKAIAPVLPHMAEDIWQNIPYEQQGESTSVFEGGFP-AGLREYGEVDAERWDLVRDLRDDVNKALEGARADKLVGASLDAAAYVHCPDEKVREILDGLVGDRDLIHPPVKSNGVDDLRTALMISQVNLVDSAEDVAEKCDDKYVMKGEK--SGCTVGVTKAAGHKCGRCWFYDETVGTTTDDSRRGPDLCQRC 1003          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A6T8JN82_HEMAN (Isoleucine--tRNA ligase n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A6T8JN82_HEMAN)

HSP 1 Score: 1258 bits (3255), Expect = 0.000e+0
Identity = 632/999 (63.26%), Postives = 758/999 (75.88%), Query Frame = 0

Query:  102 KASKQKGKKKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKAVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQ---------VETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETGEPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGER-VGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEAS--EEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDAS-LRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEE--SVSGCTVAVAPAKGDKCERCWYQCESVGTHTDHPTICSRC-HGVVQ 1084
            K  K+ GK K+D  Y  TV LP+T+F QRANAV REPELQ FW++E++YEKA E N G KFVLHDGPPYANGDLHIGHALNKILKDFINRYQIL+G K  +VPGWDCHGLPIELKVLQSMKS E+  LTP+ LR+KAA+FA+ETV KQ +SF+RYGVWGDW RPYLTL PEYEAAQ++VFG+M   GHIYRGRKPVNWSPSSRTALAEAELEYP+ HVS S++AAF VT  S AL   A  ED  + V+VWTTTPWTLPANLAVAVNE L+Y++V+ P      V  +VAKDL   + +KM  E  E+         +E +A FKG ++ G+ Y+HP+  R S+VVIGG+YITTE+GTGLVHTAPGHG EDY TG KY L LLSPVDD G FTEEAG+  KG SVL +GN+ VI  M EAGAL+K+E Y HKYPYDWRTKKPTIFRAT QWFASVD FRD A++ I +V +IPE G+ RI SMV GRSDWCISRQRNWGVPIPV YHKETGEPLLT ESI+HVR +V +KGTDAW+E+D A+LLPPS ++ A+QW +G DTMDVWFDSGSSWAGV  ER   + YP D+YLEGSDQHRGWFQSSLLTSVA+ G+APYKTVLTHGFVLDEKGFKMSKSLGN +DP+++IEGG NKK +P YGADVLRLWVS+VDY GDV VG NI+KQ+SDSYRK+RNT RYL GNL+DF+P +  V Y+++PS+DK+ML +L+ V  EV+EAFE +QFYRASQ + +  + DLSN YLD AKDRLYISSPDD  RRSCQTVL   L  LS ++AP++PHMAED WQ +P+     HKS+F  GW     P L+ +  + +KWA +R +RDDVN  +E AR    +GA++EA+  +HC D   L+A ++G      +L  P      DD+RF+L+ SQV+L  +      +    +T+G +E  + SG  V VA A G KCERCW+  ESVG H     +C RC H   Q
Sbjct:   87 KGKKKPGKSKEDSKYSKTVNLPETNFNQRANAVVREPELQAFWKDEKVYEKAYEDNTGSKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILKGSKVKFVPGWDCHGLPIELKVLQSMKSAEKRELTPVKLRKKAASFARETVDKQMDSFKRYGVWGDWERPYLTLLPEYEAAQIKVFGEMVEGGHIYRGRKPVNWSPSSRTALAEAELEYPEGHVSRSLWAAFPVTMMSEALKEKAGGED--VRVAVWTTTPWTLPANLAVAVNERLDYALVKAPS----GVCYIVAKDLVDNLSKKMQGEAEEEXXXXXXXXVLEEIAVFKGKELEGSIYKHPLYERESKVVIGGEYITTESGTGLVHTAPGHGVEDYQTGLKYNLDLLSPVDDGGLFTEEAGEDLKGKSVLGDGNQAVIDMMREAGALIKEEPYGHKYPYDWRTKKPTIFRATDQWFASVDSFRDQAMEEIAKVEFIPEKGRSRIESMVEGRSDWCISRQRNWGVPIPVFYHKETGEPLLTKESIDHVRKIVLDKGTDAWFEMDEADLLPPSHKDEADQWVKGKDTMDVWFDSGSSWAGVVQERGEDLAYPADMYLEGSDQHRGWFQSSLLTSVAAQGQAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPKQIIEGGKNKKLEPPYGADVLRLWVSSVDYTGDVRVGSNIMKQISDSYRKIRNTLRYLLGNLYDFDPAKHAVSYDEMPSVDKWMLGKLAEVQSEVKEAFEKHQFYRASQCITQLVIGDLSNLYLDVAKDRLYISSPDDLSRRSCQTVLKVALEMLSTSIAPLMPHMAEDAWQNIPWDVD--HKSVFQFGW-----PTLDYAPHDADKWATIREVRDDVNSAMELARRGGVVGANMEAKVVIHCGDNDELKALIKGSMQQDSILAYPSPVTVADDIRFILLASQVKLVDSADAVTDACGEEYTVGVKEGQTESGIVVGVAKADGKKCERCWFYDESVGEHEGQDGVCPRCGHAAAQ 1072          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: L1JBT3_GUITC (Isoleucine--tRNA ligase n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JBT3_GUITC)

HSP 1 Score: 1248 bits (3228), Expect = 0.000e+0
Identity = 624/994 (62.78%), Postives = 751/994 (75.55%), Query Frame = 0

Query:  102 KASKQKG-KKKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKAVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDL--TLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICP-RVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETGEPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVA---GERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGH-KSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLST--NGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHTDHPTICSRCHGVVQE 1085
            K SK+KG   +++G+Y STV LP T+FQQRAN+V REPE+Q+FWEE RIYEK  E N G KFVLHDGPPYANG+LHIGHALNKILKDFINRY+++ G K  +VPGWDCHGLPIELKVLQS+K +E+  +TP+ LR+KAA FAK+TV +Q ESF+RYGVW DW+ PYLTL P+YEAAQ+E FG M + GHIYRGRKPVNWSPSSRTALAEAELEYP+ H S S+YAAF V EPS A+ P        L V++WTTTPWT+PANLAVAVNE+LEYS+V H       V  VVAKDL   L    K G E+  +++ +A   G ++AGTKYQHP+   R++ VVIGGDYITTE+GTGLVHTAPGHG EDY TG KYGL LLSPVDDAGRFT EAG   +G  VLK+GNE V+  MEEAGAL+K+E Y HKYPYDWRTKKPTIFRAT QWFASV+ FR+ A+ AID V WIPEVGK RIS+MV GRSDWCISRQR+WGVPIPV YHKETGEPLLTAESI+H+R ++AE G+DAWWE D AELLPPS ++ A++W+RG DTMDVWFDSGSSW GV    GE   + +P D+YLEGSDQHRGWFQSSLLTSVA+ G APYKTVLTHGFVLDEKGFKMSKSLGN +DP  VI GG N+K +PA+GAD LRLWV++VDY GDV VG NI+KQ+SDSYRK+RNT RYL GNL  F+P + +V YEDLPS+DK+ML RL+ V +EV +A++++QFYRA QA+ +  + +LSNFYLD AKDRLYIS+ DD RRRSCQTVL A L  L+ A++P+LPHMAED WQ LP+     H  S+F  GW     P+ E    E+W  +R LRDDVN+ +E AR+ K IGA+LE    ++  D   +  +  +  +  +L S  S   N VDDLRF+ +VS V++               TL   +S SGC V VA A+G KCERCW+  ++VGTH     IC RC   V++
Sbjct:    7 KGSKKKGGNDEEEGIYSSTVNLPVTNFQQRANSVVREPEIQKFWEENRIYEKLYESNPGTKFVLHDGPPYANGNLHIGHALNKILKDFINRYKVISGHKVKFVPGWDCHGLPIELKVLQSLKKEEKENMTPISLRKKAAEFAKQTVDQQRESFKRYGVWADWSEPYLTLDPKYEAAQIETFGAMLKGGHIYRGRKPVNWSPSSRTALAEAELEYPEGHKSRSMYAAFTVVEPSEAVKP----HSENLKVAIWTTTPWTIPANLAVAVNEKLEYSIVEH-----HGVKYVVAKDLKGALAAKLKKGEEDEVELKEIAVITGKELAGTKYQHPLYKERINPVVIGGDYITTESGTGLVHTAPGHGVEDYQTGLKYGLELLSPVDDAGRFTAEAGPLLEGKDVLKDGNERVLELMEEAGALIKEEEYGHKYPYDWRTKKPTIFRATDQWFASVENFREKAMGAIDTVKWIPEVGKNRISAMVEGRSDWCISRQRSWGVPIPVFYHKETGEPLLTAESIDHIRDIIAEHGSDAWWEKDVAELLPPSHKQEADKWERGRDTMDVWFDSGSSWNGVVKSWGEGKALDFPADMYLEGSDQHRGWFQSSLLTSVAAQGTAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPALVINGGKNQKTEPAFGADTLRLWVASVDYTGDVRVGGNIMKQISDSYRKIRNTLRYLLGNLHGFDPKKHSVKYEDLPSVDKWMLGRLARVQEEVRDAYDTFQFYRAYQAILQVCITELSNFYLDIAKDRLYISAEDDVRRRSCQTVLHAALEMLTTAISPMLPHMAEDAWQNLPWE----HVSSVFEHGWKQLDYPSHEG---ERWDYIRSLRDDVNQCMEAARADKLIGATLEGSVAIYVADEEKKQLVTSLLHEVSMLTSETSPGFNKVDDLRFLFLVSDVKIVDTPEDVTSLCNEKHTLSISDSASGCFVGVARAQGKKCERCWFYSDTVGTHEGLENICPRCAQAVKQ 984          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A1Z5JP77_FISSO (Isoleucine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JP77_FISSO)

HSP 1 Score: 1239 bits (3205), Expect = 0.000e+0
Identity = 613/981 (62.49%), Postives = 743/981 (75.74%), Query Frame = 0

Query:  110 KKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKAVEGNQG-GKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETG-EPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDF----NPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHT-DHPTICSRCHGVV 1083
            KK+  V+ ST+ILP T F QRANAV REPELQ +W+E  +Y K  + N   G+FVLHDGPPYANGDLH GHALNKILKDF+NR Q+L G++ HY+PGWDCHGLPIELKVLQ+M SKER+GLTP+ LR+KAAAFAKETV KQS +FQR+G++GD+ +PYLTLQPE+EAAQ+ VFG+MF++G+I+RGRKPV+WSPSSRTALAEAELEYPD HVS S+Y AF V +PS AL     A+D  L V++WTTTPWT+PANLAVAVN +L YSVV H K G     L+VA DL  T+ +K  L EGE  +   TF+G D+ GT YQHP+  R S V+ GGDYITTE+GTGLVHTAPGHGQEDYLTG K GL +LSPVDD G+FT +AG++F GLSVL EGN+ +I A+ E GALLK E Y HKYPYDWRTKKPTIFRAT QWFASV  FRD+AL+AID V W+P+VGK RI++ V+GR DWCISRQR+WGVPIPV Y + TG E LL  ++++H++ L  + G+D WW +D  +LLP S +  AE+W +GTDTMDVWFDSGSSWAGVA  R  + YP D+YLEGSDQHRGWFQSSLLTSVA+  +APYKTVLTHGFVLDEKGFKMSKSLGN +DP +VI+GGNN+K +PAYGADVLRLWV+N DY+ DV +G NIIKQ  +SYRKLRNT RY+ GNL DF     PG + VPY+DLPS+DK+ML RL+ V+K V+EA   YQF RA Q + RFA ADLSNFYLD AKDRLYIS  DD RRRSCQTV+   L   +KAM+P+LPHMAED+WQ LP+     H S+F  GW  A   A      ++W  VR LRDDVNK+LE AR+ K +GASL+  AFV+  D   RA LE + GD+ L+  P+ TNGVD+LR  LM+SQV L  +      + E  +  G +++ SGC V V  A G+KC RCW+  E +G H   H   C RC+  +
Sbjct:   45 KKEKNVFASTIILPDTPFSQRANAVVREPELQAYWKESGLYHKLSKQNAASGRFVLHDGPPYANGDLHCGHALNKILKDFVNRKQLLNGKQVHYIPGWDCHGLPIELKVLQTMSSKERSGLTPVTLRQKAAAFAKETVEKQSVAFQRFGIYGDFDKPYLTLQPEFEAAQIRVFGEMFKKGYIFRGRKPVHWSPSSRTALAEAELEYPDGHVSKSIYVAFNVDKPSQALVEHHSAQDP-LKVAIWTTTPWTMPANLAVAVNSDLSYSVVFHEKTG----KLLVATDLAETLAKKFALPEGEVFDVRGTFQGSDLVGTTYQHPLYERRSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLEILSPVDDVGKFTADAGEKFVGLSVLAEGNQAIIDALAETGALLKAEDYGHKYPYDWRTKKPTIFRATDQWFASVQGFRDSALKAIDTVKWVPDVGKNRITAFVSGRGDWCISRQRSWGVPIPVFYDRATGKEVLLNEDTLQHIQELFVKHGSDCWWTMDEKDLLPESYKHEAEKWVKGTDTMDVWFDSGSSWAGVAQSRNELAYPADMYLEGSDQHRGWFQSSLLTSVANNDQAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPMQVIKGGNNQKLEPAYGADVLRLWVANCDYSSDVSIGPNIIKQTFESYRKLRNTARYMIGNLADFVPEGQPGSNAVPYDDLPSMDKWMLGRLTAVLKLVDEAMNEYQFQRAVQEILRFATADLSNFYLDVAKDRLYISGIDDFRRRSCQTVIQKCLEGFAKAMSPLLPHMAEDIWQNLPYET--SHDSVFEGGWP-ADLAAYPEFAVDEWDLVRELRDDVNKVLEAARNDKLVGASLDGAAFVYVADDRKRAILEKLVGDENLISPPVKTNGVDELRTALMLSQVSLVGSETALKEACEEKYISG-KDTASGCIVGVRKASGEKCGRCWFYDEQIGKHNLPHGDACQRCNEAI 1016          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: A0A7S0LI09_9EUKA (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0LI09_9EUKA)

HSP 1 Score: 1238 bits (3203), Expect = 0.000e+0
Identity = 614/971 (63.23%), Postives = 729/971 (75.08%), Query Frame = 0

Query:  112 DDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKAVEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGG-LWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETGEPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALE--ASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHTDHPTICSRC 1079
            D G YRS+V LPQT F QRAN+  RE ELQRFWEE+ +YE+  + N G KFVLHDGPPYANGDLHIGHALNKILKD INRYQ+L+G++  YVPGWDCHGLPIELKVLQ MKSKER  LTP+ LR KAAAFA+ETV+KQ ESF+RYGVWG+W  PYLTLQPEYEAAQ+ VFGKM  +GHIYRGRKPV+WSPSSRTALAEAELEYPD HVSTS+Y A +VTEPS ALAP+    DG  + V++WTTTPWT+PANLAVAVN +L+YSVV H  L      LVVA DL  +V  ++ L EGE++ T+A   G D+  + Y+HP+  R S+VVIGGDYITTE+GTGLVHTAPGHGQEDY+TGQKYGLPLLSPVDDAG+FT EAG+ F GLSVL +GN  VI A++EAGALL  E Y HKYPYDWRTKKPTIFRATSQWFASVD FRD+AL AI+ V W+P +G+ RI+SM   RSDWCISRQR WGVPIPV Y ++T E LL   +++HV+AL AE G+D WW+ D   LLP   +  A +W++GTDTMDVWFDSGSSW+GVA  R  + YP DLYLEGSDQHRGWFQSSLLTSVA+ G APYKTVLTHGFVLDEKG+KMSKSLGN +DPR VIEGGNNKK +P YGADVLRLWV++V+YA DVC+G +IIKQ  +SYRKLRNT R+L GNL DF P   TVPY++LP  D+YML  L+ VM E + AF+ YQF RA  A+Q+F VADLSNFYLD  KDRLYIS+P + RRRSCQTVL      L+ A+APILPH+AED+W  LP+ PP    S+F + W     P L+   S+   W+ VR LRD  N+ +E AR  KA+GASLEA+  +H  D  L+  L   +G+        + NGVD+LRF  + SQV L      +   A+T+      E     TV VA A G KCERCW+    + +   +P +C RC
Sbjct:   43 DGGAYRSSVSLPQTSFSQRANSATRELELQRFWEEQGVYERLRDSNPGEKFVLHDGPPYANGDLHIGHALNKILKDIINRYQMLQGKRVCYVPGWDCHGLPIELKVLQGMKSKERQALTPISLREKAAAFAQETVSKQRESFKRYGVWGEWDSPYLTLQPEYEAAQIGVFGKMALKGHIYRGRKPVHWSPSSRTALAEAELEYPDKHVSTSIYVALKVTEPSEALAPYTT--DGTPVAVAIWTTTPWTIPANLAVAVNADLKYSVVSHASL---DFKLVVASDLVSSVAGRLSLPEGEELSTIAVLSGADLEKSVYKHPLYERTSQVVIGGDYITTESGTGLVHTAPGHGQEDYITGQKYGLPLLSPVDDAGKFTTEAGEPFVGLSVLGDGNTAVITALQEAGALLASEGYAHKYPYDWRTKKPTIFRATSQWFASVDAFRDDALSAIESVRWLPAIGQNRITSMTQSRSDWCISRQRTWGVPIPVFYDRDTDEVLLNKATLDHVQALFAEHGSDVWWQQDEEFLLPDELKSEAARWRKGTDTMDVWFDSGSSWSGVADSRSKLSYPADLYLEGSDQHRGWFQSSLLTSVAANGVAPYKTVLTHGFVLDEKGYKMSKSLGNVIDPRSVIEGGNNKKTQPGYGADVLRLWVASVNYAADVCIGPSIIKQTFESYRKLRNTARFLLGNLHDFVPASHTVPYDELPETDRYMLGLLAEVMAEAKAAFDDYQFSRALGAIQQFVVADLSNFYLDVGKDRLYISAPGEARRRSCQTVLSITTHALAAALAPILPHLAEDIWLNLPYKPP--TTSVFENPW-----PQLDYQVSDAAAWSAVRELRDVANRCIELARIDKALGASLEAKLLLHVEDPELKEALRRWQGEGA------AGNGVDELRFFFLTSQVELLET---AEAVAQTSLHSIVREEAPAATVGVAKADGHKCERCWHYHVDLASSVSYPNVCPRC 992          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: B7FRF4_PHATC (Isoleucine--tRNA ligase n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FRF4_PHATC)

HSP 1 Score: 1231 bits (3184), Expect = 0.000e+0
Identity = 618/985 (62.74%), Postives = 741/985 (75.23%), Query Frame = 0

Query:  108 GKKKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEK-AVEGNQGG--KFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVVRHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAGDRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETG-EPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERVGMGYPVDLYLEGSDQHRGWFQSSLLTSVASTGKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDT----VPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHT-DHPTICSRCHGVV 1083
            G KK    Y  T++LP+T F QRANAV REPELQ  W+E  +Y K + +  + G  +FVLHDGPPYANGDLH GHALNKILKDFINR QIL G++ HY+PGWDCHGLPIELKVLQ+MKSKER  LTP+ LR KAA FAKETV KQS +FQRYG++GD+ +PYLTLQPE+EAAQ+ VFG+MF++G+I+RGRKPV+WSPSSRTALAEAELEYP+ HVS S+Y AF V  PS  LA F   E+  L V++WTTTPWT+PAN+AVAVN EL YSVV+H K G     L+VA DL  ++ +K  L E E ++ LATF G D+ GT Y+HP+  R S V+ GGDYITTE+GTGLVHTAPGHGQEDYLTG K GL +LSPVDD G+FT EAG+R+ G+ VL EGN  +I A++EAG+LLK E Y HKYPYDWRTKKPTIFRAT QWFASV  FRD AL A+++V W+PEVGK RI S V GR DWCISRQR+WGVPIPV Y K TG E LL  +++ HV+ L AE G+D WW++D +ELLP   R  A++WQ+GTDTMDVWFDSGSSWAGVA  R  + YP D+YLEGSDQHRGWFQSSLLTSVA+   APYKTVLTHGFVLDEKGFKMSKSLGN +DP +VIEGGNNKKQ PAYGADVLRLWV+N DY+GDV +G NIIKQ  DSYRKLRNT RYL GNL DF P + T    V Y+DLPS+DK+ML RLS V++ V EA + +Q+ RA Q L RFA ADLSNFYLD AKDRLYIS+ DD RRRSCQTVL A L   +KA++PILPHMAED+WQ LP+       S+F  GW      +    + + W  VRL+RDDVNKMLE ARS K +GASL+A A+++ PD+  +A LE + GD  L+  P+ TNGVD+LR  LM+SQ+ L         + E  + + S ++ SGC V V  A G KC RCW+  E VG H+  +  +C RC+  +
Sbjct:   53 GAKKPKNAYAPTIVLPETAFSQRANAVIREPELQALWKETNLYHKLSSQAKEAGAERFVLHDGPPYANGDLHCGHALNKILKDFINRKQILNGKQVHYIPGWDCHGLPIELKVLQTMKSKEREALTPVTLREKAAEFAKETVEKQSVAFQRYGIFGDFEKPYLTLQPEFEAAQIRVFGEMFQKGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSIYVAFNVDTPSEVLASFHSTEEP-LKVAIWTTTPWTMPANMAVAVNPELSYSVVQHEKTG----KLLVATDLASSLARKFDLPEDETLDILATFPGKDLVGTIYKHPMYDRKSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLEILSPVDDVGKFTIEAGERYVGMRVLAEGNLAMIDALQEAGSLLKAEDYGHKYPYDWRTKKPTIFRATDQWFASVAGFRDQALDAVEKVKWVPEVGKNRIRSFVDGRGDWCISRQRSWGVPIPVFYDKATGKEVLLNKDTLAHVQTLFAEHGSDCWWKMDESELLPEKFRAEADKWQKGTDTMDVWFDSGSSWAGVAQSRDELAYPADMYLEGSDQHRGWFQSSLLTSVANNNIAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPLKVIEGGNNKKQDPAYGADVLRLWVANSDYSGDVLIGDNIIKQTFDSYRKLRNTARYLIGNLADFVPSDSTDSNAVAYDDLPSMDKWMLGRLSAVLRTVNEAMDDFQYQRAIQELLRFASADLSNFYLDVAKDRLYISAMDDARRRSCQTVLYACLEGFTKAISPILPHMAEDIWQNLPYQK--STDSVFEGGWP-TNLMSYAEFDSQTWDLVRLVRDDVNKMLESARSDKLVGASLDAAAYIYVPDSEKKAILEKLVGDASLVAPPVKTNGVDELRTALMLSQIHLVENENDVREACEDKY-VSSRDTSSGCIVGVGRAVGTKCARCWFYDEEVGNHSLTYADVCQRCNEAI 1028          

BLAST of mRNA_S-ischiensis_contig97.21133.1 vs. uniprot
Match: K0T838_THAOC (Isoleucine--tRNA ligase n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0T838_THAOC)

HSP 1 Score: 1221 bits (3160), Expect = 0.000e+0
Identity = 609/991 (61.45%), Postives = 742/991 (74.87%), Query Frame = 0

Query:  108 GKKKDDGVYRSTVILPQTDFQQRANAVKREPELQRFWEEERIYEKA---VEGNQGGKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILRGRKAHYVPGWDCHGLPIELKVLQSMKSKERAGLTPLDLRRKAAAFAKETVAKQSESFQRYGVWGDWARPYLTLQPEYEAAQLEVFGKMFREGHIYRGRKPVNWSPSSRTALAEAELEYPDDHVSTSVYAAFQVTEPSAALAPFAVAEDGGLWVSVWTTTPWTLPANLAVAVNEELEYSVV-RHPKLGDRQVNLVVAKDLTLTVGQKMGLEEGEQVETLATFKGGDIAGTKYQHPICPRVSEVVIGGDYITTEAGTGLVHTAPGHGQEDYLTGQKYGLPLLSPVDDAGRFTEEAG------DRFKGLSVLKEGNEEVIVAMEEAGALLKQEAYPHKYPYDWRTKKPTIFRATSQWFASVDRFRDNALQAIDEVTWIPEVGKKRISSMVTGRSDWCISRQRNWGVPIPVMYHKETG--EPLLTAESIEHVRALVAEKGTDAWWELDTAELLPPSERESAEQWQRGTDTMDVWFDSGSSWAGVAGERV----GMGYPVDLYLEGSDQHRGWFQSSLLTSVAST-GKAPYKTVLTHGFVLDEKGFKMSKSLGNTLDPREVIEGGNNKKQKPAYGADVLRLWVSNVDYAGDVCVGQNIIKQVSDSYRKLRNTCRYLTGNLFDFNPGEDTVPYEDLPSLDKYMLARLSGVMKEVEEAFESYQFYRASQALQRFAVADLSNFYLDTAKDRLYISSPDDPRRRSCQTVLMAMLMDLSKAMAPILPHMAEDVWQTLPFHPPGGHKSIFHSGWVGAGGPALEASEEEKWAKVRLLRDDVNKMLEQARSTKAIGASLEAQAFVHCPDASLRATLEGMRGDQRLLPSPLSTNGVDDLRFVLMVSQVRLYSAFVQSGVSAETTFTLGSEESVSGCTVAVAPAKGDKCERCWYQCESVGTHTDHPTICSRCHG 1081
            G KK    Y  T++LPQTDF QRANA+KREPELQ +W+   +Y K     E     +FVLHDGPPYANGDLHIGHALNK+LKDFINR+QIL+G++ HYVPGWDCHGLPIELKVLQ+MKSKER GLTP+ LR +AA FAKETV KQS SFQRYGV GD+  PYLTL PEYEAAQ+ VFG+M+++G I+RGRKPV+WSPSSRTALAEAELEYP+ HVS S+Y A  V  PS  L        G L V++WTTTPWT+PANLAVA+N ELEY VV  HP + D Q  L+VAK L  ++  K  L +G+ ++ +A+F G  + GT YQHP+  R S V+ GGDYITTE+GTGLVHTAPGHGQEDYLTG K GL LLSPVDD GRFT EAG      D   G SVL EGN  VI A+EEAGALLK E Y HKYPYDWRTKKPTIFRAT QWFASV+ FRD+AL+A+D V WIP+VGK RI+S +  R DWCISRQR+WGVPIPV Y KETG  + LL  +++EH++++ AE+G+DAWW+LD  +LLP   +  A++W +GTDTMDVWFDSGSSWAGV  ER     G+ YP D+YLEGSDQHRGWFQSSLLTSVA+  G+APYK VLTHGFVLDEKGFKMSKSLGN ++P +VIEGGNN+K +PAYGADVLRLWVS+VDYAGDV VG NIIKQ  +SYRKLRNT RYL GNL D+NP  D +PY+DLPS+DK+ML  L+ V+ EV++A+ +YQF RA+  + RFA ADLSNFYLD AKDRLYIS+ DD RRRSCQTVL  +L   +K+ APILPHMAED+WQ LP+   G + S+F  G V A   +    ++E+W  +R +R D N++LE AR  K +GASL+A A+++  D  +R  L  + GD+ ++   + TNGVD+LR  LM+SQV L  + +    + ++T+T   E  +SGCT+ V  A+G KC RCW+   +VG H        +CHG
Sbjct:   55 GGKKPKNAYADTILLPQTDFSQRANAIKREPELQEYWKSIDLYSKLSSDAESRSAERFVLHDGPPYANGDLHIGHALNKLLKDFINRHQILKGKQVHYVPGWDCHGLPIELKVLQTMKSKERQGLTPIALRERAATFAKETVEKQSASFQRYGVIGDFDNPYLTLLPEYEAAQIRVFGEMYKQGFIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSIYVALDVVSPSEELQQHV--GSGNLKVAIWTTTPWTIPANLAVAINPELEYCVVDAHPNVLDGQSKLLVAKGLVESLETKFDLSDGDNLKVIASFNGSSLVGTSYQHPLYERQSPVIAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLELLSPVDDVGRFTVEAGSSTIVGDDLVGKSVLGEGNIAVIEALEEAGALLKAEDYGHKYPYDWRTKKPTIFRATDQWFASVEGFRDDALKAVDSVQWIPDVGKNRINSFIESRGDWCISRQRSWGVPIPVFYDKETGGTQVLLDEDTLEHIQSIFAEQGSDAWWKLDVVDLLPNKYKSEADKWTKGTDTMDVWFDSGSSWAGVVQERANKEKGLSYPADIYLEGSDQHRGWFQSSLLTSVAANKGQAPYKAVLTHGFVLDEKGFKMSKSLGNVVNPLQVIEGGNNRKLEPAYGADVLRLWVSSVDYAGDVRVGSNIIKQTFESYRKLRNTARYLIGNLDDYNPSTDAIPYDDLPSMDKWMLGTLTKVLNEVDDAYSNYQFSRATNEILRFATADLSNFYLDVAKDRLYISAIDDFRRRSCQTVLHKLLEGFAKSFAPILPHMAEDIWQNLPYKAEGDNSSVFEGG-VPAELLSYSEFDKERWELIRDVRTDANQVLELARQDKLVGASLDAAAYIYTEDEIIRKVLGELDGDEHIISPAVKTNGVDELRTALMISQVHLVDSELDVTSACDSTYTAKGE--LSGCTIGVKKAEGKKCGRCWFYDTNVGPH-------GKCHG 1033