mRNA_S-ischiensis_contig91.20889.1 (mRNA) Schizocladia ischiensis KU_0333

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: D7FPP9_ECTSI (Alanine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPP9_ECTSI)

HSP 1 Score: 1262 bits (3266), Expect = 0.000e+0
Identity = 628/967 (64.94%), Postives = 758/967 (78.39%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAGGRPMVLEAEQTAALASMGVEATDASAKYVWHHEPSAKVLALFVGKGEGANGSGFVEKVSGAGE-----VVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQEVPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVG-GGKGGGKVDRAQVIATGVGSVEAAMQAA 3159
            W A ++R +FI++F +KH HTFW SSPVVPHDDPTL+FANAGMNQFKPLFLGTADP+LPLSKLTRA NSQKCIRAGGKHNDLDDVGKD YHHTFFEMLGNWSFGSYFKEEAI +AWECLT V+ LDP+RLYATYFGGDE+L +G D++A++IWLK+LP  RVLPF   DNFWEMGDVGPCGPC EIHYDR GGRDAA  VN D P+VIEIWN VFIQYNRE DGSL+ELP+KH+DTG+GFERLASILQGK SNYDTD+F PIF+AI++ATGA PYTG +G+ED D+ DMAYRVV+DHIRTLT+AITDG  PSN+GRGYVLRR+LRR +RYGQ +LGG+PGFFH LVP VV +MKG FPEL   +A+V  V+KDEE +FN+TLDKGLK F+K+A  L    GDS++FPG AAF LYSSMGFP+DL TLMAEEKGL +D  GF + M++EK +   A + +K AGG+PM+LEAEQ   L+    EAT+AS +Y WH +P+AKVLALFVGKGEG +G GFV   S   +      VGVVL+ TSFYAE GGQ++D G L+ SDG+ S A+  V +FG Y LHIGQ+++G L VG+ V C VDYERRA +APNHTMTHVLNFALR+VL       + GMCDQKGSF+DD+KLRFDFSW+G+LT EQV  VE +VND I+K LPVYA+VAPLEQA RIQSLR VFGERYPDPVRVLS+GQ+VPAMLEDPEN AWS +SVEFCGGTHLSNT+EA  F +LEEAGIAKG+RRI+ VT  LA  A++ A AF+ +L   +  + + ++   +RL   L+   +S   KN+ R R++ L+++A   KK+ M  +V  AV     +AE  K  G T+++FR D GCDAK A ++L +V K  PE   +MLFTAD + DR+LC G IPN+KA  KD + WV+   DA G GGKGGGK DRAQ I+ GV +++ AM+ A
Sbjct:   31 WPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRENDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAIRSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAAL----GDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSDEKTKSNQARSNQKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDIGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYERRANVAPNHTMTHVLNFALRKVLD-----PSDGMCDQKGSFLDDEKLRFDFSWSGSLTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAQRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQCALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVA 988          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: A0A6V2XN65_HETAK (Alanine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V2XN65_HETAK)

HSP 1 Score: 1195 bits (3092), Expect = 0.000e+0
Identity = 613/972 (63.07%), Postives = 741/972 (76.23%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAGGRPMVLEAEQTAALASMGVEATDASAKYVWHHEPS---AKVLALFVGKGEGANG-SGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESG-ELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQEVPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNL---IKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAMQAAMDY 3168
            W A++IR +FI +F D+++H FWPSSPVVPHDDPTL+FANAGMNQFKP FLGT DPSLP++KL+RAVNSQKCIRAGGKHNDLDDVGKD YHHTFFEMLGNWSFG+YFK EAIAWAWECLT VFGL P RLYATYFGGDE  GL PD+EA+EIWL+FLP ERVLPFG KDNFWEMGD GPCGPCTEIHYDR GGRDAAPLVNADLPDVIEIWNNVFIQ+NRE  G LRELP+KHVDTGMGFERLASILQGKTSNYDTDIF+PIF+AIQ  TGA  YTG +  ED D+ DMAYRVV+DHIRTLT AITDGAVPS++GRGYVLRR+LRRGVRYGQ +LGG PGFFH+LVP VV +MKGAFPELEGRQA VM+V+K+EE++FN+TLDKGL+ F+KIA+++ +      +FPGDAA FLY++MGFPVDLTTLMAEE+GL +D  GF   M EE+++  +A AA K+ GG  +VLEAEQT+ LA+ GV ATD++ KY+   E +   A V+A+F  K E  NG +GFV+  + A   VGV+L+ T+FYAE GGQ+FDTG +A  +   +  + N Q F GY LH+G++  G  L VG +V   VDY RRA++APNHTMTHVLNFALREVLLGGV+    G CDQKGS VD ++LRFDF+WNG L  EQ+  VE IVN+ I ++LPVYA V+P EQA +I SLR+VFGERYPDPVRV+SVG  V  +L DP +P W+GLS+EFCGGTHL+NT +A  F L+EE+GIAKGIRR+I VT   A+EA D + AF+ ELKA  + +   + E  K L++ LN LA+S   KNR RE +  L   +K   K + +AM+    G  EE  K+A  A   G  +V+ RVD GCD KV  + + ++ K +PE    M+ +AD D DR+ C   IP AK +G DA+AW+ A  DA GGGK GGK D A     G   ++AA  AA  +
Sbjct:   18 WPAERIRESFIAFFRDRYEHVFWPSSPVVPHDDPTLLFANAGMNQFKPHFLGTMDPSLPMAKLSRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGAYFKREAIAWAWECLTEVFGLSPERLYATYFGGDETQGLEPDEEAREIWLQFLPAERVLPFGCKDNFWEMGDTGPCGPCTEIHYDRIGGRDAAPLVNADLPDVIEIWNNVFIQFNREEGGVLRELPNKHVDTGMGFERLASILQGKTSNYDTDIFQPIFRAIQQVTGARDYTGKIEAEDTDNVDMAYRVVADHIRTLTFAITDGAVPSSDGRGYVLRRVLRRGVRYGQQVLGGPPGFFHQLVPVVVGLMKGAFPELEGRQAFVMDVIKEEEESFNKTLDKGLREFNKIAEQVKAE--GKTVFPGDAAQFLYATMGFPVDLTTLMAEEQGLTLDAEGFEAKMEEEREKSKEAHAAAKSGGGANLVLEAEQTSWLANNGVAATDSAPKYLGAEEGAGLPAVVVAIFK-KSEVDNGRAGFVDSATAADGPVGVLLDRTAFYAESGGQIFDTGAIAFGEAGEAFEVGNCQTFAGYDLHVGEVAGGGSLAVGAAVRVGVDYARRARVAPNHTMTHVLNFALREVLLGGVTDDRTGACDQKGSAVDAERLRFDFAWNGPLEPEQLARVEAIVNEKIAEQLPVYAEVSPFEQACQIPSLRRVFGERYPDPVRVISVGAPVGELLADPLDPKWNGLSIEFCGGTHLTNTGQAAFFQLMEESGIAKGIRRVIAVTRGAAEEARDRSRAFEAELKAAAELEGEALLEACKGLTQALNPLAISVVDKNRLREELGALATKVKAWKKKQLKAMSGMALGLCEEKAKEAREA---GARKVVLRVDFGCDGKVGKKCVQEMHKHHPEGV-FMVLSADEDADRFQCYAMIPGAKEKGLDANAWLAAAMDAAGGGKSGGKPDNAMGTVNGCSGIDAAAAAAEAF 982          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: A0A835Z4U5_9STRA (Alanine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4U5_9STRA)

HSP 1 Score: 1192 bits (3084), Expect = 0.000e+0
Identity = 612/983 (62.26%), Postives = 735/983 (74.77%), Query Frame = 1

Query:  250 DGVGKGAEVWTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAGGRPMVLEAEQTAALASMGVEATDASAKYVWHHEPSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSV-----------TCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTN--RGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQEVPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQ---SDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAM 3150
            D +      W A++IR TFI +F  K QHTFWPSSPVVPHDDPTL+FANAGMNQFKPLFLGT DPSLPLSKLTRA NSQKCIRAGGKHNDLDDVGKD YHHTFFEMLGNWSFG YFK+EAI WAWECLTV FGL+  RLYATYFGGD  LGL PD+EA+ IWLK+LP ERVLPFG KDNFWEMGD GPCGPCTEIHYDR GGRDAA LVNAD PDVIEIWNNVFIQ+NRE  G L+ELPSKHVDTGMGFERLASILQGK SNYDTD+F PIF  IQ+ TGA PY+G +G+ED D+ DMAYRVV+DHIRTLT AITDGAVPSN+GRGYVLRRILRRGVRYGQ ILG +PGFFH+LVP VV+++KGAFPEL+ R + VM++LKDEE  FNRTLDKGL+ F K A ++ ++ G  ++FPG  AF LYS+MGFPVDLT LMAEE GL +D   +H  M E+K + A AE AR+ AGG  + +EAEQT+ALA+ GV  T++ AKY WHH PSAKV+AL+ GKG+GA G GFVE+    GE VG++L+ T FYAEQGGQV+DTG L + D  +SV + NVQ +GGYVLH G +    L VGT+V           TC VDY+RR+++APNHTMTHVLN+ALR VL GG+   +  +G+CDQKGSFVDD KLRFDF+W+G L  +Q+ AVEKIVN+TI  ++PVYA+VAPL++A +I++LR VFGERYPDPVRVLSVGQEVPAMLEDP++ +WS LSVEFCGGTHL+NT +A  FA++EE GIAKGIRRII VT + A EA   A  F+ E+KA+      D LK+E++ KRL   L++ A+SA  K R R+ I+         KK+A    V  A E      E AK  G+ RVI  +  G D K     L      + + + ++ F +D D  R+ C+  +P      K   AW++A   +VGG   GGK D+A    +G+ +V AA+
Sbjct:    9 DSIAVDVNGWPAERIRKTFIEFFEQK-QHTFWPSSPVVPHDDPTLLFANAGMNQFKPLFLGTVDPSLPLSKLTRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKQEAITWAWECLTVTFGLNAERLYATYFGGDPALGLAPDEEAKAIWLKYLPAERVLPFGCKDNFWEMGDTGPCGPCTEIHYDRIGGRDAAVLVNADRPDVIEIWNNVFIQFNRESGGVLKELPSKHVDTGMGFERLASILQGKDSNYDTDVFMPIFGVIQSVTGARPYSGKVGEEDTDTVDMAYRVVADHIRTLTFAITDGAVPSNDGRGYVLRRILRRGVRYGQQILGAKPGFFHQLVPVVVNMLKGAFPELKNRVSFVMDILKDEETAFNRTLDKGLREFSKAATKVKAT-GGPKVFPGTTAFLLYSAMGFPVDLTQLMAEEAGLTVDMVEYHAKMEEDKAKSAAAEVARRAAGGTALTMEAEQTSALAAKGVAGTESEAKYTWHHTPSAKVVALYAGKGKGALGDGFVEEAV-EGERVGIILDTTPFYAEQGGQVYDTGSLDAED--LSVEVVNVQTYGGYVLHTGIVSGKALAVGTTVXXXXXXXXXXXTCSVDYDRRSRVAPNHTMTHVLNYALRSVLCGGLDNKSVAQGLCDQKGSFVDDDKLRFDFAWSGPLPADQIAAVEKIVNETIQAKMPVYAHVAPLDKALKIRALRSVFGERYPDPVRVLSVGQEVPAMLEDPDSDSWSNLSVEFCGGTHLTNTGDAEAFAIMEETGIAKGIRRIIAVTRERAHEAKALAGTFEEEVKALAPLATQDPLKLEKETKRLLPVLDTAAISATDKQRIRDIIAGYTVAITAHKKKAANEGVAAAKELGTTAGEAAKAAGKDRVIVVLPDGADNKTITAALTAAKAAHGDVKALLGFASDPDAGRYACVAVMPGTSGAAK---AWLEAALSSVGGTASGGKGDKAVGFGSGIENVAAAI 983          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: W7TIB5_9STRA (Alanine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TIB5_9STRA)

HSP 1 Score: 1163 bits (3008), Expect = 0.000e+0
Identity = 599/972 (61.63%), Postives = 707/972 (72.74%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADEL-------ASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAGGRPMVLEAEQTAALASMGVEATDASAKYVWHHEPSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQEVPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAMQAAMDYA 3171
            W+A ++R TFI+YF +K +H FWPSSPVVPHDDPTL+FANAGMNQ+KPLFLG  DP LPLSKLTRAVNSQKCIRAGGKHNDL+DVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAW+CL   FGLDP+RLYATYFGG+E+ GL  D+EA+ IWL++LP  RVLPF  KDNFWEMG+ GPCGPCTEIHYDR   R+   LVN D PD+IEIWN VFIQYNRE  G+LRELPSKHVDTGMGFERL SILQ K SNYDTDIF P+F AIQ  TGAPPY G LG  DP+SRDMAYRVV+DHIRTLT AI DGAVPSN+GRGYVLRRILRR VRYG  +LG  PGFF KLV  VV+ MK  FPELE ++ +V+EVL DEE+ FNRTLD+G KHF K+ADEL          KG+   FP   A +LYS+MGFPVDLT LMAEEKGL +D AGF   +AE+K++ A A AA+K  GG+ +VLEAEQTA L S GVE TD+S KYVWHHEPSA+V+AL+ GK  G     FVE+V+    VV V+L  TSFYAEQGGQ+ DTGV+ + DGT ++ +++VQ++GGYVLHIG LESG L VG + TCMVDYERRA+IAPNHTMTHVLN+ALR VLL GVS    G+CDQKGS VDD+KLRFDF+WN ALT+E++  VE IV + I +ELPV++Y+APLE+A  I SLRQVFGERYPDPVRVLSVG EVP MLEDP NPAW   SVEFCGGTHLSNT+EA  FAL+EE GIAKGIRRI  VT D+A+ A + A  F   L   E     ++E + K L+  L  L +SA  K RFR  +  L+ +  K K                                R+D G D K+A   LDK  +++P     +L +AD D  ++      P   +   D  AWV A  +  GGG+ GGK  R Q +  G+  + +A+ AA  +A
Sbjct:   26 WSARRVRQTFIDYFTNKREHVFWPSSPVVPHDDPTLLFANAGMNQYKPLFLGIVDPKLPLSKLTRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWDCLVNEFGLDPSRLYATYFGGNEKQGLTADEEARNIWLRYLPPNRVLPFDCKDNFWEMGETGPCGPCTEIHYDRLPAREVPELVNMDSPDLIEIWNVVFIQYNREEGGALRELPSKHVDTGMGFERLTSILQDKPSNYDTDIFMPLFDAIQELTGAPPYAGKLGKADPESRDMAYRVVADHIRTLTFAIADGAVPSNDGRGYVLRRILRRAVRYGAKVLGAAPGFFSKLVHVVVNQMKDIFPELEEKEKRVVEVLADEERAFNRTLDQGEKHFKKVADELLLAASQRGGDKGEKVAFPAKEAHYLYSTMGFPVDLTELMAEEKGLTVDRAGFETLLAEDKRQSAMAAAAKKEMGGKEIVLEAEQTAWLQSRGVEITDSSLKYVWHHEPSARVMALYKGKAVG-----FVEEVTAEDGVVAVILNTTSFYAEQGGQIHDTGVITNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYERRARIAPNHTMTHVLNYALRRVLLKGVS-DGSGLCDQKGSAVDDEKLRFDFAWNKALTKEELAEVETIVRERISQELPVHSYIAPLEKALHINSLRQVFGERYPDPVRVLSVGHEVPPMLEDPTNPAWQDTSVEFCGGTHLSNTREAERFALIEETGIAKGIRRITAVTKDMARAAEELAEEFAGLLALAEVKPEEELEGEVKMLNTRLGPLTISAVEKERFRTALDALLTRVKKFKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERLDFGTDGKIAKEALDKASQIHPSG-CFLLISADEDSGKFGVYSTAPKLGSP-LDPIAWVAAAVEGAGGGRSGGKSPRGQGVGEGLEKIGSAVTAAKAFA 989          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: A0A7S4ME88_9STRA (Alanine--tRNA ligase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4ME88_9STRA)

HSP 1 Score: 1048 bits (2711), Expect = 0.000e+0
Identity = 553/965 (57.31%), Postives = 680/965 (70.47%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARK-NAGGRPMVLEAEQTAALASMGVEATDASAKYVWHHE-PSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLG-GVSWTNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQE-VPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAMQAA 3159
            W  D++RSTFI++F  KH HTFWPSSP VPHDDPTL+FANAGMNQ+KPLFLGT DP+L LSKL RAVNSQKCIRAGGKHNDLDDVGKD YHHT FEMLGNWSFG YFKE AI  AW+CLT  F LD +RLYATYF GD +  +  D EA++IWLK+LP ERV+P G +DNFWEMG VGPCGPCTEIHYDR GGRDAA LVNADLPDVIEIWNNVFIQYNRE DGSLR LP++HVDTGMGFERL SILQGK SNYDTDIF P+F AIQ  TGAP Y+G +G ED   RDMAYRVV+DHIRTL+ AI DGA PSN+GRGYVLRR+LRR VRYG+  LG + GFF  LVPT+V +M G FPEL+  QA V  V+KDEE++F++TLDKGL  F ++AD +    GD ++FPG  A FLY+SMGFPVDLT LMAEE GL +D  GF   M +E+ + A+A   +    GG+ M L AEQTA L + GV+ATD  AKYVW+ +    K  ALFVG+GE  +G GF + ++G    VG++L+ TSFYAE GGQ +DTGV+ ++ G V V I+NVQ++G +VLH+GQ+  G + +G  + C VDY RR  IA NHTMTHVLN+ALR+VL+  G + T + + DQKGS VD+ KLRFDFSWNG L   Q+ AVE ++N  I   +P+ ++VAPLE A +I SLR VFGE YPDPVRV+SV  E +P +L++P++  W   SVEFCGGTHLSNTKEA  F LL+E GIAKG+RRI  VT   A+ A + A  F+  LK        ++E   K+LSE L  L++S   K+ F+  +  L K+A   KK+  A +    VE+ V  A  A   G  ++  R+D G D KVA  V     K   + +  +L TAD    R++ +   P       D  AW +A  +  G  KGGGK D AQ  ATG+  +E  ++ A
Sbjct:   13 WPMDRVRSTFIDFFAKKHGHTFWPSSPCVPHDDPTLLFANAGMNQYKPLFLGTCDPNLELSKLKRAVNSQKCIRAGGKHNDLDDVGKDVYHHTMFEMLGNWSFGDYFKEGAIDMAWQCLTEEFKLDTSRLYATYFAGDSQTPV--DDEARKIWLKYLPPERVIPCGAEDNFWEMGAVGPCGPCTEIHYDRIGGRDAAALVNADLPDVIEIWNNVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQGKDSNYDTDIFMPLFAAIQKLTGAPAYSGKVGAEDEGFRDMAYRVVADHIRTLSFAIADGATPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFSTLVPTLVDLMGGTFPELKKHQAHVTAVIKDEEESFSKTLDKGLLRFKELADRV----GDDKVFPGADAHFLYTSMGFPVDLTELMAEELGLTLDREGFDAKMKDEQDKSAEAHRLKMMGKGGKDMRLVAEQTAYLVNKGVDATDDEAKYVWNKDLEGCKAAALFVGRGETEDGIGFADSITGENGAVGLILDKTSFYAEAGGQTYDTGVIKTAGGAV-VRIDNVQSYGQFVLHVGQVTEGTVALGEELICSVDYVRRTPIASNHTMTHVLNYALRDVLVTKGDNKTGQDV-DQKGSLVDELKLRFDFSWNGPLKPAQLAAVEDMINSRISAGVPIQSFVAPLEDAKQISSLRAVFGEVYPDPVRVVSVSPEPIPEILQNPQDGQWLQYSVEFCGGTHLSNTKEAEMFVLLKEEGIAKGVRRITAVTGGDAKAAGERADEFEGRLKEAGALPGAELEAGVKKLSEELKDLSISVARKDGFKTELGKLTKKAMAWKKEQAAARTKEVVEQLVSAANEA---GGNKIALRIDFGIDGKVAKSVAQSFAKKVKD-KAFLLITADESSGRFMVVASAPKGMKDPVDCKAWCQAATEGTGA-KGGGKKDLAQFNATGIEKIEGVLEKA 964          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: A0A7S4GJP2_9EUGL (Alanine--tRNA ligase n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S4GJP2_9EUGL)

HSP 1 Score: 1038 bits (2685), Expect = 0.000e+0
Identity = 545/971 (56.13%), Postives = 673/971 (69.31%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSL--PLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGP-DKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAGGRPMVLEAEQTAALASMGVEATDASAKYVWHHEPSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTNR--GMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQEVPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGK-GGGKVDRAQVIATGVGSVEAAMQAAMDYA 3171
            W  +++R TFI+YF  KH+HTFWP+SPV P DDPTL+F NAGMNQFKPLFLG ADP++   L+ LTRAVNSQKCIRAGGKHNDL+DVGKDTYHHTFFEMLGNWSFGSYFK+EAI WAWECLT  +GLDP+R+YATYFGGD +    P D+EA+++WL+FLP ERV+P G KDNFWEMGD GPCGPCTEIHYDR  GR    LVNAD P++IEIWN VFIQ+NRE DG LR LP++HVDTGMGFER+ SILQ K SNYDTDIF PIF+AIQ ATGA PY G LG+EDPD+ DMAYRVV+DHIRTLT AI DGA PSNEGRGYVLRR+LRR VRYGQ +LGG+PGFFH+LVPT+V  MK  FP++  R   VM ++KDEE  FN+TL KG+ HF K+ +EL +   D + F  + A FLY+S+GFPVDLT LMAEE+GL++D  GF + M EE +  A A   RK AGG+ + L AEQTA L   GVE TDA  KY WH  PSA V ALF+G GEG  G GFV+    +   VGVVL+ + FY E GGQV DTG L    G  ++ + N Q +GG+VLH G + +GEL VG +V C VDYERRA +APNHTMTHVLN ALR+VL GGV       G+ DQKGS  D+QKLRFDFSWNG +  EQ++ VE  VN  ID   PV A    LEQA  I SLR VFGE+YPDPVRV+SVG  V AM+++P+N  W+  S+EFCGGTH++N+ EA  F ++EE+GIAKG+RRI+ VT   A+EAI  A A   ++KAV      +++   K   + L +  V    K    + +  LIK+  K +KQ    K    + + +   E AK  G+  V+ R+D G D K+ +++L +  K Y  +   M+F+A +D  R+      P     G DA AW  A  +     K  GG      ++      ++A M AA ++A
Sbjct:   79 WPMNRVRQTFIDYFAKKHEHTFWPTSPVAPLDDPTLLFTNAGMNQFKPLFLGIADPAVHGKLATLTRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKKEAIPWAWECLTQDYGLDPSRMYATYFGGDPKDPSVPVDEEARDLWLQFLPEERVIPCGAKDNFWEMGDTGPCGPCTEIHYDRREGRLVPELVNADDPELIEIWNIVFIQFNREDDGQLRPLPAQHVDTGMGFERITSILQNKDSNYDTDIFTPIFEAIQEATGARPYAGKLGEEDPDNIDMAYRVVADHIRTLTFAIADGASPSNEGRGYVLRRVLRRAVRYGQQVLGGKPGFFHQLVPTIVETMKDGFPDIVNRADFVMSIIKDEEFAFNKTLGKGVAHFEKVVNELPA---DDKTFKAEDAHFLYASLGFPVDLTELMAEERGLVLDREGFAKKMKEEAEISAIAAQQRKLAGGKDLRLGAEQTAHLQQTGVEVTDAGDKYNWHVTPSATVKALFIGVGEGEGGLGFVDVADASTGPVGVVLDASPFYPEAGGQVADTGRL---QGPGTMHVENTQIYGGFVLHTGSVPTGELRVGDAVQCAVDYERRAHVAPNHTMTHVLNHALRDVLCGGVEGAAEMGGLVDQKGSLCDEQKLRFDFSWNGPVGPEQLQRVEAQVNACIDAAQPVDALPVALEQAKFIYSLRSVFGEQYPDPVRVVSVGNNVSAMVDEPQNKDWAAGSIEFCGGTHIANSAEAERFVIMEESGIAKGVRRIVAVTRSKAREAIANAAALREKVKAVAALPDAELDAAVKVFQKELLATPVGVVDKGALGKEVETLIKRVKKYQKQLEKEKEAEVLGKILPAVEEAKENGKGVVVLRLDFGTDGKLGSKLLKEATKAYKAS--YMVFSASSDGTRFGIYASTPKG---GLDAKAWCAAAGEPSNAAKVNGGPTQANAMVIRANQHLDACMAAAEEFA 1038          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: A0A7S3L1R5_9STRA (Alanine--tRNA ligase n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3L1R5_9STRA)

HSP 1 Score: 1033 bits (2672), Expect = 0.000e+0
Identity = 553/976 (56.66%), Postives = 685/976 (70.18%), Query Frame = 1

Query:  250 DGVGKGAEVWTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAG-GRPMVLEAEQTAALASMGVEATDASAKYVWHHE-PSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQE-VPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAMQAAMDY 3168
            D   K    W   K+R+TFI +F  KH H FWPSSP VP DDPTL+F NAGMNQ+KPLFLGT DP+L +SKL RAVNSQKCIRAGGKHNDLDDVGKD YHHTFFEMLGNWSFG YFK+EAI  AWECL   F +DP RLYATYF GDE   +  D+EA++IWL++LP ERVLPF  KDNFWEMG  GPCGPCTEIHYDR GGRDAA LVNADLPDVIEIWNNVFIQYNRE DGSLR LP++HVDTGMGFERL SILQG  SNYDTDIF P+F+AIQ  TG  PY G +G ED D  DMAYRVV+DHIRTL  AITDGAVPSN+GRGYVLRR+LRR VRYG+  LG + GFF +LVPTVV ++   FPEL  ++ QV  +++DEE++F+RTLDKGL  F ++A+++    GD ++F  D A FLY++MGFP+DLT LMAEEKGL +D  GF R M +EK+    A  A+++ G G+ M L AEQTA L + GVEAT+   KYVW+ E     V ALF+G+ E  +  GFV+++S     VGV+L+ TSFYAE GGQV+DTG + S+ G   + + NVQ +G YVLH+G++ SG    G +V+C VDY RR  IA NHTMTHVLNFALR+VL    S T     DQKGSF D+ K RFDFSWNG +T EQ+  VEK+  + I+KE+PV +YVAPL +A  I SLR VFGE+YPDPVRV++V    +P ML +P++  W+  +VEFCGGTHL NTKEA  FALL E GIAKGIRRI+ VT   A++AI AA  F+ +L+  +Q D   +E + K LS+ +  L  SA  K++FR+ IS+L K+    KK+ +A   D  VE+ V  A   +T G  +VIFR D G D K+    L   +    + + +++ +AD D D+ L     P   A+  D  AW+ A      G KGGGK D AQ    GV + ++ +  A ++
Sbjct:    5 DNNDKAPLEWPMKKVRATFIEFFQRKHGHDFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMSKLKRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEAIDMAWECLVHEFQMDPNRLYATYFAGDETTPV--DEEARQIWLQYLPPERVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGGRDAAKLVNADLPDVIEIWNNVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQGVDSNYDTDIFMPLFKAIQRVTGCRPYGGKVGAEDADFVDMAYRVVADHIRTLCFAITDGAVPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFAQLVPTVVELLSDTFPELREKEDQVTAIIRDEEESFSRTLDKGLLKFKELAEKV----GDDKVFSADDAHFLYTTMGFPIDLTELMAEEKGLKVDKDGFERKMQQEKEISDAARLAKQSGGSGKDMRLVAEQTAHLVTKGVEATNDEPKYVWNQELTGCTVKALFIGRNETEDKIGFVDEISPESSTVGVILDKTSFYAEAGGQVYDTGFIKSASGA-QIKVENVQMYGQYVLHLGEVVSGTFKTGETVSCSVDYVRRGPIAANHTMTHVLNFALRKVLASDGSIT----IDQKGSFQDEFKSRFDFSWNGQVTTEQLAEVEKLCIERIEKEVPVESYVAPLAKAQEISSLRAVFGEKYPDPVRVVAVSDHTIPEMLGNPQDATWNEYAVEFCGGTHLKNTKEAEAFALLSEEGIAKGIRRIVFVTQADAKKAIAAAAEFESKLEQAKQLDGEVLEAECKVLSQEVMGLTASAIKKSQFRDTISDLQKKVIAYKKEKIAGMTDEIVEKMV--AAAGETSGN-KVIFRFDFGSDGKMVKN-LTSAYGKQVQDKAILILSADEDTDKLLVAAFAPKG-AKDVDCKAWLTAATQGTDG-KGGGKKDMAQFHVPGVTNADSILTKAKEF 963          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: A0A7S1ZG71_TRICV (Alanine--tRNA ligase n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZG71_TRICV)

HSP 1 Score: 1019 bits (2635), Expect = 0.000e+0
Identity = 548/970 (56.49%), Postives = 674/970 (69.48%), Query Frame = 1

Query:  274 VWTADKIRSTFINYFIDK-HQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAG-GRPMVLEAEQTAALASMGVEATDASAKYVWHHE-PSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSWTNR-GMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQE-VPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAMQAAMDY 3168
            VWT D +RSTF++YF      HT  PSSP VP  DPTL+FANAGMNQFKP+FLGT DP+ P+S   RA NSQKCIRAGGKHNDLDDVGKD YHHT FEMLGNWSFG YFKE AI  AW+CL   F LD +RLYATYF GDE+    PD+EA+ IWLK+LP +RVLPF  KDNFWEMG  GPCGPCTEIHYDR GGRDAA LVNADLPDVIEIWNNVFIQYNRE DGSLR LP++HVDTGMGFERL SILQGK SNYDTDIF P+F  I+  TGA PY G +G +D    DMAYRV++DHIRTL+ AI DGAVPSN+GRGYVLRR+LRR VRYG+  LG   GFF +LVPT+V +M   FPEL+  Q  V  +++DEE++F++TLDKGL  F     E+AS  GD ++F GD AFFLY+SMGFPVDLT LMAEE GL +D  GF   M EE+ + A+A   +   G G+ M L AEQTA L S GVEATD  AKY+W+ E       ALFVG+GE  +G GFV  ++    +VG+VL+ T+FYAE GGQ++DTG + +S G V V ++NVQA+G +VLHIG++  G + +G  + C VDY+RR+ IA NHTMTHVLNFAL++VL+     T++    DQKGS VD+ KLRFDFSW G LT  Q+ AVE  VND I   +P+ +YVAPL++A  I SLR VFGE YPDPVRV+SV  E +P +LE+P++  W   S+EFCGGTHLSNT EA  F LL+E GIAKGIRRI GVT   AQ A + A AF  +L    + D   +E   K +SE LN L++SA  K  F+++++ L K+    KK+  A +     +  +  A  + T+G  +V+ R D G D KVA  V     K   + +  +L TAD D DR+L +   P    +G D  AW  A  +  G  KGGGK D AQ  ATGV  ++  ++ A ++
Sbjct:   85 VWTTDAVRSTFVDYFASSPRSHTRVPSSPCVPLSDPTLLFANAGMNQFKPIFLGTVDPNTPMSDWKRAANSQKCIRAGGKHNDLDDVGKDVYHHTMFEMLGNWSFGDYFKEGAIDMAWQCLVEEFKLDASRLYATYFAGDEQTP--PDEEARRIWLKYLPPDRVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGGRDAASLVNADLPDVIEIWNNVFIQYNREADGSLRSLPAQHVDTGMGFERLTSILQGKDSNYDTDIFMPLFAEIEKITGAKPYGGKVGADDEGFVDMAYRVIADHIRTLSFAIADGAVPSNDGRGYVLRRVLRRAVRYGRQNLGADLGFFAQLVPTLVGLMGETFPELKKHQDHVTAIIRDEEESFSKTLDKGLVRFK----EMASQVGDDKVFNGDDAFFLYASMGFPVDLTELMAEELGLTLDREGFDAKMKEEQDKSAEAHRKKMMGGSGKDMRLVAEQTARLVSDGVEATDDEAKYMWNQELGGCAAKALFVGRGETEDGVGFVPSINAESGMVGLVLDKTAFYAEAGGQIYDTGSIKTSGGGV-VRVDNVQAYGQFVLHIGEVTEGTVNLGDELICCVDYKRRSPIAANHTMTHVLNFALKDVLV--TKGTDKIASVDQKGSLVDESKLRFDFSWAGPLTGAQLGAVEDAVNDRIRSAIPIQSYVAPLDKAKLISSLRAVFGEVYPDPVRVVSVSPEPIPEILENPQDGQWLDYSIEFCGGTHLSNTSEAGSFVLLKEEGIAKGIRRIFGVTGSDAQAATERADAFASKLDDTSKMDGDAMEGALKSMSEELNGLSISAARKEGFKDKLTELTKKVLSWKKEQAAARTKEVAQAVI--AAASSTEGD-KVVIRNDFGIDGKVAKAVKGAFDKKVKD-KAFLLVTADVDADRFLVVASAPKGM-KGVDCKAWASAATEGTGA-KGGGKKDLAQFNATGVDKIDGVLEKARNF 1039          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: B7G5M3_PHATC (Alanine--tRNA ligase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7G5M3_PHATC)

HSP 1 Score: 1017 bits (2629), Expect = 0.000e+0
Identity = 545/971 (56.13%), Postives = 671/971 (69.10%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAG-GRPMVLEAEQTAALASMGVEATDASAKYVWHHE-PSAKVLALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLGGVSW----TNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQE-VPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGGGKGGGKVDRAQVIATGVGSVEAAMQAAMDY 3168
            W  +K+R TF NYF+++H H FWPSSP VP DDPTL+F NAGMNQ+KPLFLGT DP+L +SKLTRAVNSQKCIRAGGKHNDLDDVGKD YHHTFFEMLGNWSFG YFK  AI  AW+CLTV FGLDP RLYATYF GDE   +  D+EA+++WL++LP +RVLPF  +DNFWEMG  GPCGPCTEIHYDR GGRDA+ LVNADLPDV+EIWN VFIQYNRE DGSLR LP++HVDTGMGFERL SILQ   SNYDTDIF P+F AIQ  TGA PY   +G EDP+  DMAYRVV+DHIRTL  AITDGAVPSN+GRGYVLRR+LRR VRYG+  LG + GFF KLVP  V VM  AFPE+  +Q  V  ++++EE++F+RTLDKGL+ F+++A+++    G  Q+F G  A FLY+SMGFPVDLT LMAEEKG+ ++   F   M EE      A  A+   G G+ M L AEQT+ L    + ATD +AKYVWH E     V A F+G+ E  +  GFV  +S     VG+VL+ +SFYAE GGQV+D G L SS G V V I NVQA+G +VLH+G++ SG L VG +V C VDY RRA IA NHTMTHVLN ALREVL+         T+    DQKGS VD+ KLRFDFSW+G LT EQ+  VEK+  D I   +PV AYVAPL  A +I SLR VFGE+YPDPVRV++V    VP ML +P++  W+  SVEFCGGTHL+NTKEA  F LL E GIAKGIRRI  +TT  A++AI  A  F+ +L A E      +E   K+LS  L+ L ++A  K +FRE+++ + KQ    KKQ +A                    G ++V+ R D G + KVA  V+    K   + + ++L TAD + DR++ + G P A     +  AW++A  D +   KGGGK D AQ   +GV +V+  ++ A  +
Sbjct:   15 WPMEKVRDTFKNYFVEQHGHVFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMSKLTRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKAGAIDMAWQCLTVTFGLDPERLYATYFAGDELTPV--DEEARQLWLRYLPDDRVLPFDARDNFWEMGATGPCGPCTEIHYDRIGGRDASKLVNADLPDVMEIWNVVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQNVDSNYDTDIFIPLFTAIQNITGARPYAKKVGKEDPEYIDMAYRVVADHIRTLCFAITDGAVPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFAKLVPAFVDVMGSAFPEVVEKQEYVTGIIQEEEESFSRTLDKGLQKFNELAEKV----GADQIFSGADAHFLYTSMGFPVDLTELMAEEKGMTLNKEEFEAKMQEEHDISQAAHLAKMAGGSGKDMRLVAEQTSYLVGQNISATDDAAKYVWHEELADCVVKACFIGRNETEDMIGFVGSISPESSAVGIVLDKSSFYAEAGGQVYDVGTLTSSTGAV-VKITNVQAYGQFVLHLGEVASGTLSVGDTVKCSVDYVRRAPIASNHTMTHVLNHALREVLIKRPEKESGKTSTLTVDQKGSLVDETKLRFDFSWSGQLTPEQLAEVEKLCMDRIVNAVPVDAYVAPLGDAQQISSLRAVFGEKYPDPVRVVAVSDHAVPEMLANPQDSQWNEYSVEFCGGTHLTNTKEAEAFVLLAEEGIAKGIRRITAITTGEAKKAIALANEFESKLTAAEVVQGDDLESTVKQLSAELDGLDIAAVKKMQFREQLATMTKQVLAYKKQKLA--------------------GGSKVVMRFDFGVEGKVAKSVMTAFGKQVKD-KALLLVTADPEADRFMVIAGAPKAMKD-LNCKAWIEAATDGLDA-KGGGKPDSAQYQVSGVEAVDTVLEKARKF 955          

BLAST of mRNA_S-ischiensis_contig91.20889.1 vs. uniprot
Match: B8C356_THAPS (Alanine--tRNA ligase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C356_THAPS)

HSP 1 Score: 1006 bits (2600), Expect = 0.000e+0
Identity = 540/970 (55.67%), Postives = 676/970 (69.69%), Query Frame = 1

Query:  277 WTADKIRSTFINYFIDKHQHTFWPSSPVVPHDDPTLMFANAGMNQFKPLFLGTADPSLPLSKLTRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWECLTVVFGLDPTRLYATYFGGDERLGLGPDKEAQEIWLKFLPTERVLPFGPKDNFWEMGDVGPCGPCTEIHYDRTGGRDAAPLVNADLPDVIEIWNNVFIQYNREPDGSLRELPSKHVDTGMGFERLASILQGKTSNYDTDIFEPIFQAIQAATGAPPYTGMLGDEDPDSRDMAYRVVSDHIRTLTMAITDGAVPSNEGRGYVLRRILRRGVRYGQDILGGQPGFFHKLVPTVVSVMKGAFPELEGRQAQVMEVLKDEEQTFNRTLDKGLKHFHKIADELASSKGDSQLFPGDAAFFLYSSMGFPVDLTTLMAEEKGLIMDTAGFHRAMAEEKKRGADAEAARKNAG-GRPMVLEAEQTAALASMGVEATDASAKYVWHHEPSAKVL-ALFVGKGEGANGSGFVEKVSGAGEVVGVVLELTSFYAEQGGQVFDTGVLASSDGTVSVAINNVQAFGGYVLHIGQLESGELLVGTSVTCMVDYERRAKIAPNHTMTHVLNFALREVLLG-GVSWTNRGMCDQKGSFVDDQKLRFDFSWNGALTQEQVEAVEKIVNDTIDKELPVYAYVAPLEQAARIQSLRQVFGERYPDPVRVLSVGQE-VPAMLEDPENPAWSGLSVEFCGGTHLSNTKEARCFALLEEAGIAKGIRRIIGVTTDLAQEAIDAATAFDIELKAVEQSDILKVEEQAKRLSETLNSLAVSAPFKNRFRERISNLIKQANKAKKQAMALKVDGAVEEAVKQAEMAKTQGQTRVIFRVDVGCDAKVANRVLDKVFKVYPEAERVMLFTADTDKDRWLCLGGIPNAKAQGKDASAWVKATADAVGG--GKGGGKVDRAQVIATGVGSVEAAMQAAMDY 3168
            W  DK+RSTFI+YF +++ HTFW SSP VPHDDPTL+F NAGMNQ+KPLFLGT DP+L ++ L RA NSQKCIRAGGKHNDLDDVGKD YHHTFFEMLGNWSFG YFK+EAI  AW+CLT  F LDP RLYATYFGGDE      D EA+EIWL++LP ERVLPF  KDNFWEMG  GPCGPCTEIHYDR G RDA+ LVNADLPDVIEIWNNVFIQ+NRE DGSLR LP++HVDTGMGFERL SILQG  SNYDTDIF P+F AIQ  TGA PY G +  EDP+  DMAYRVV+DH+RTL+ AI DGAVPSN+GRGYVLRR+LRR VRYG+  L  + GFF +LV T+V +M   FPEL  ++  V  ++++EE++F++T+DKG+  F+K  D L    GD ++FPG+ A FLY+SMGFPVDLT LM EE+GL +D  G+   M EE++    A  A+ +AG G+ M L AEQT+ L + GV+AT+  AKYVW+ +    ++ ALF+G+ E  +G GFV+ +S     VG+VL+ T+FY E GGQ FDTG + +S G  ++ I NVQ +G +VLHIG +  G + V  + TC VDY RRA IA NHTMTH+LNFALR+VL+  G +  +    DQKGS VD+ KLRFDFSW+G LT EQ+ AVEKI  D I+  +PV  YVAPLE A +I SLR VFGE YPDPVRV++V    + +MLE+P++  W+  SVEFCGGTHL NT EA+ F LL E GIAKG+RRI GVT   A  A  AA+ F+ +L A    + L +E   K L+  +  L++SA  K +FR+ +    K+    KK   A +    VE+ ++ A    T+G  +V+ R D G D KVA  V     K   + + ++  TAD D DR++ +   P  K    D  AWV A   AV G  GKGGGK D AQ    GVG+++  ++ A  +
Sbjct:   14 WPMDKVRSTFISYF-ERNGHTFWRSSPCVPHDDPTLLFTNAGMNQYKPLFLGTCDPALKMASLKRATNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEAIEMAWKCLTEEFKLDPNRLYATYFGGDESTPC--DDEAREIWLQYLPAERVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGNRDASKLVNADLPDVIEIWNNVFIQFNREADGSLRPLPAQHVDTGMGFERLTSILQGVDSNYDTDIFIPLFDAIQKITGARPYAGKIEKEDPEFIDMAYRVVADHVRTLSFAIADGAVPSNDGRGYVLRRVLRRAVRYGRQNLNAELGFFAQLVRTLVELMGDTFPELREKEEYVTAIIREEEESFSKTVDKGIVEFNKRCDAL----GDVKVFPGEDAHFLYTSMGFPVDLTELMCEERGLTLDMEGYDAKMKEEQELSNAAHLAKMSAGSGKDMRLVAEQTSHLVNKGVDATNDDAKYVWNEDLEGSIIKALFIGRNETEDGIGFVDSISKENGAVGIVLDKTNFYGESGGQTFDTGSIKTSSGA-TIEITNVQIYGSFVLHIGDVTEGTVTVNDAATCCVDYVRRAPIAANHTMTHILNFALRDVLVTRGDADQHGASVDQKGSLVDENKLRFDFSWSGPLTPEQLVAVEKICVDQINSAVPVDNYVAPLEGAQQISSLRAVFGEVYPDPVRVVAVSPSPITSMLENPQDAQWNKYSVEFCGGTHLKNTSEAQGFVLLSEEGIAKGVRRITGVTMKDAAIATAAASEFEAKLTAAGSLEGLDLEAHVKVLTVEMEGLSISAAKKIQFRDVMGTYGKKVAAWKKANAAEQTATVVEQVIEAAN--STEGN-KVVVRCDFGVDGKVAKAVSTAYGKKIKD-KALLFVTADVDADRFMVVAFAP--KGSDVDCKAWVAA---AVAGTDGKGGGKKDSAQSTVPGVGNIDGVLEKARGF 966