mRNA_S-ischiensis_contig11.2014.1 (mRNA) Schizocladia ischiensis KU_0333

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-ischiensis_contig11.2014.1
Unique NamemRNA_S-ischiensis_contig11.2014.1
TypemRNA
OrganismSchizocladia ischiensis KU_0333 (Schizocladia ischiensis KU_0333)
Homology
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A835YGL0_9STRA (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase chloroplast n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YGL0_9STRA)

HSP 1 Score: 134 bits (337), Expect = 1.260e-34
Identity = 66/91 (72.53%), Postives = 78/91 (85.71%), Query Frame = 1 
Query:    1 VALEDCTKYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLFDK 273
            + L+  TKYCESV  T+RRPTRTVW GKVPIGSEHR+ALQTMTTTDTR+V ATV+QV+ CA+AGADLVRITVQG +EA+A  E+R +LF K
Sbjct:   10 LTLDQMTKYCESVTATRRRPTRTVWAGKVPIGSEHRIALQTMTTTDTRNVAATVDQVMRCADAGADLVRITVQGNKEAEAAAEVRDQLFKK 100
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A7S3QZ12_DUNTE (Hypothetical protein n=1 Tax=Dunaliella tertiolecta TaxID=3047 RepID=A0A7S3QZ12_DUNTE)

HSP 1 Score: 133 bits (335), Expect = 2.580e-34
Identity = 66/82 (80.49%), Postives = 75/82 (91.46%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            KYCE+V+ TKRRPTRTV  GKVPIGS+HRVALQTMTTTDT+DV+ATV QV  CA+AGADLVRITVQG++EAKAC EIRQ+LF
Sbjct:   51 KYCETVHATKRRPTRTVMAGKVPIGSDHRVALQTMTTTDTKDVEATVAQVKKCADAGADLVRITVQGRREAKACQEIRQRLF 132
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A835XZX4_9CHLO (Uncharacterized protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A835XZX4_9CHLO)

HSP 1 Score: 131 bits (330), Expect = 1.100e-33
Identity = 62/82 (75.61%), Postives = 76/82 (92.68%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            KYCES+Y TKRRPTRTV +GKVP+GS+HR+ALQTMTTTDTR+V  TV+QV  CA+AGAD+VRITVQGK+EA+ACM+IR++LF
Sbjct:    6 KYCESIYQTKRRPTRTVMIGKVPVGSQHRIALQTMTTTDTRNVQMTVDQVKKCADAGADIVRITVQGKKEAEACMKIREQLF 87
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: D8U477_VOLCA (Uncharacterized protein n=4 Tax=Volvox TaxID=3066 RepID=D8U477_VOLCA)

HSP 1 Score: 131 bits (329), Expect = 1.510e-33
Identity = 63/82 (76.83%), Postives = 75/82 (91.46%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            KYCESVY T+RRPTRTV +GKVP+GSEHR+ALQTMTTTDTR+V  TV+QV  CA+AGAD+VRITVQGK+EA+ACM IR++LF
Sbjct:    5 KYCESVYQTQRRPTRTVMIGKVPVGSEHRIALQTMTTTDTRNVQLTVDQVKKCADAGADIVRITVQGKKEAEACMRIREQLF 86
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: D7FRY5_ECTSI (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, chloroplast n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FRY5_ECTSI)

HSP 1 Score: 130 bits (328), Expect = 2.160e-33
Identity = 66/88 (75.00%), Postives = 74/88 (84.09%), Query Frame = 1 
Query:    1 VALEDCTKYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKL 264
            V LE+ TKYCE    TKRR +RTVW GKVPIGSEHR+A QTMTTT+T DVDATV QVI CA+AGADLVRITVQGK+EAK+C  IR+KL
Sbjct:   12 VGLEEWTKYCEDTSATKRRTSRTVWAGKVPIGSEHRIARQTMTTTNTNDVDATVAQVIKCADAGADLVRITVQGKREAKSCKLIREKL 99
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A8J5XID9_DIALT (Uncharacterized protein n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XID9_DIALT)

HSP 1 Score: 130 bits (328), Expect = 2.280e-33
Identity = 62/84 (73.81%), Postives = 75/84 (89.29%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLFDK 273
            +YCESVYT  RRPTRTVWVGKVPIGSEH +A+QTMTTTDTRDV+A+VEQ+I CA+AGADLVR+TVQG QEA+AC  I+ +L ++
Sbjct:   47 RYCESVYTPTRRPTRTVWVGKVPIGSEHPIAIQTMTTTDTRDVEASVEQIIRCADAGADLVRLTVQGMQEARACALIKARLLER 130
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A7S0RJS8_9CHLO (Hypothetical protein n=1 Tax=Chlamydomonas leiostraca TaxID=1034604 RepID=A0A7S0RJS8_9CHLO)

HSP 1 Score: 130 bits (327), Expect = 3.130e-33
Identity = 61/82 (74.39%), Postives = 77/82 (93.90%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            KYCES++ T+RRPTRTV +GKVP+GS+HRVALQTMTTTDTR++ ATV+QV  CA+AGAD+VRITVQGK+EA+ACM+IR++LF
Sbjct:   44 KYCESIHQTRRRPTRTVMIGKVPVGSQHRVALQTMTTTDTRNIQATVDQVKKCADAGADIVRITVQGKKEAEACMKIREQLF 125
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A061S1H7_9CHLO ((E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase n=2 Tax=Tetraselmis sp. GSL018 TaxID=582737 RepID=A0A061S1H7_9CHLO)

HSP 1 Score: 129 bits (323), Expect = 1.100e-32
Identity = 61/82 (74.39%), Postives = 73/82 (89.02%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            +YCES + T RRPTRTVWVGKVPIGSEH +  QTMTTTDTRDV ATV+QV+ CA+AGAD+VRITVQG++EA+AC EIR++LF
Sbjct:   50 QYCESTFQTMRRPTRTVWVGKVPIGSEHPIVKQTMTTTDTRDVQATVDQVMKCADAGADMVRITVQGRREAEACCEIRERLF 131
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A7S3FQP6_9CHLO (Hypothetical protein n=1 Tax=Chloropicon roscoffensis TaxID=1461544 RepID=A0A7S3FQP6_9CHLO)

HSP 1 Score: 129 bits (323), Expect = 1.110e-32
Identity = 61/81 (75.31%), Postives = 73/81 (90.12%), Query Frame = 1 
Query:   25 YCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            YCE++Y T+RRPT TVWVG VPIGSEH +A QTMTTTDT++VDATVEQV+ CA+AGADLVR+TVQG +EA+ACM IR+KLF
Sbjct:   60 YCETIYKTQRRPTSTVWVGNVPIGSEHPIARQTMTTTDTKNVDATVEQVMKCADAGADLVRVTVQGGREAEACMHIREKLF 140
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BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Match: A0A150GJ77_GONPE (Uncharacterized protein n=1 Tax=Gonium pectorale TaxID=33097 RepID=A0A150GJ77_GONPE)

HSP 1 Score: 128 bits (322), Expect = 1.400e-32
Identity = 61/82 (74.39%), Postives = 75/82 (91.46%), Query Frame = 1 
Query:   22 KYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDVDATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLF 267
            KYCE+V+ TKRRPTRTV +GKVP+GS+HR+ALQTMTTTDTR+V  TV+QV  CA+AGAD+VRITVQGK+EA+ACM IR++LF
Sbjct:    5 KYCETVHQTKRRPTRTVMIGKVPVGSDHRIALQTMTTTDTRNVQLTVDQVKKCADAGADIVRITVQGKKEAEACMRIREQLF 86
The following BLAST results are available for this feature:
BLAST of mRNA_S-ischiensis_contig11.2014.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YGL0_9STRA1.260e-3472.531-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate syn... [more]
A0A7S3QZ12_DUNTE2.580e-3480.49Hypothetical protein n=1 Tax=Dunaliella tertiolect... [more]
A0A835XZX4_9CHLO1.100e-3375.61Uncharacterized protein n=1 Tax=Edaphochlamys deba... [more]
D8U477_VOLCA1.510e-3376.83Uncharacterized protein n=4 Tax=Volvox TaxID=3066 ... [more]
D7FRY5_ECTSI2.160e-3375.001-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate syn... [more]
A0A8J5XID9_DIALT2.280e-3373.81Uncharacterized protein n=2 Tax=Diacronema lutheri... [more]
A0A7S0RJS8_9CHLO3.130e-3374.39Hypothetical protein n=1 Tax=Chlamydomonas leiostr... [more]
A0A061S1H7_9CHLO1.100e-3274.39(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synth... [more]
A0A7S3FQP6_9CHLO1.110e-3275.31Hypothetical protein n=1 Tax=Chloropicon roscoffen... [more]
A0A150GJ77_GONPE1.400e-3274.39Uncharacterized protein n=1 Tax=Gonium pectorale T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-ischiensis_contig11contigS-ischiensis_contig11:447970..450187 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Schizocladia ischiensis KU_03332021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score132.9
Seed ortholog evalue8e-29
Seed eggNOG ortholog3067.XP_002953482.1
Preferred nameHDS
KEGG rclassRC01486
KEGG koko:K03526
KEGG ReactionR08689,R10859
KEGG Pathwayko00900,ko01100,ko01110,ko01130,map00900,map01100,map01110,map01130
KEGG ModuleM00096
GOsGO:0001101,GO:0002376,GO:0003674,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006081,GO:0006082,GO:0006090,GO:0006629,GO:0006644,GO:0006720,GO:0006793,GO:0006796,GO:0006950,GO:0006952,GO:0006955,GO:0007154,GO:0007165,GO:0008150,GO:0008152,GO:0008299,GO:0008610,GO:0008654,GO:0009058,GO:0009240,GO:0009507,GO:0009526,GO:0009532,GO:0009536,GO:0009570,GO:0009605,GO:0009607,GO:0009617,GO:0009627,GO:0009719,GO:0009725,GO:0009751,GO:0009814,GO:0009862,GO:0009863,GO:0009941,GO:0009987,GO:0010033,GO:0014070,GO:0016491,GO:0016725,GO:0019288,GO:0019637,GO:0019682,GO:0019752,GO:0023052,GO:0031967,GO:0031975,GO:0032787,GO:0032870,GO:0035690,GO:0042221,GO:0042493,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0045087,GO:0046429,GO:0046490,GO:0046677,GO:0048037,GO:0050789,GO:0050794,GO:0050896,GO:0051536,GO:0051539,GO:0051540,GO:0051704,GO:0051707,GO:0051716,GO:0052592,GO:0055114,GO:0065007,GO:0070887,GO:0071229,GO:0071236,GO:0071310,GO:0071407,GO:0071446,GO:0071495,GO:0071704,GO:0090407,GO:0098542,GO:1901135,GO:1901576,GO:1901700,GO:1901701
EggNOG free text desc.GcpE protein
EggNOG OGs2QSBY@2759,34GRU@3041,37P9A@33090,COG0821@1
EC1.17.7.1,1.17.7.3
COG Functional cat.I
Best tax levelChlorophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Hectar predicted targeting categoryother localisation
Exons2
Model size287
Cds size285
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815589.2784715-CDS-S-ischiensis_contig11:447969..4481371622815589.2784715-CDS-S-ischiensis_contig11:447969..448137Schizocladia ischiensis KU_0333CDSS-ischiensis_contig11 447970..448137 +
1695739788.2825687-CDS-S-ischiensis_contig11:447969..4481371695739788.2825687-CDS-S-ischiensis_contig11:447969..448137Schizocladia ischiensis KU_0333CDSS-ischiensis_contig11 447970..448137 +
1622815589.2909236-CDS-S-ischiensis_contig11:450068..4501851622815589.2909236-CDS-S-ischiensis_contig11:450068..450185Schizocladia ischiensis KU_0333CDSS-ischiensis_contig11 450069..450185 +
1695739788.2945526-CDS-S-ischiensis_contig11:450068..4501851695739788.2945526-CDS-S-ischiensis_contig11:450068..450185Schizocladia ischiensis KU_0333CDSS-ischiensis_contig11 450069..450185 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-ischiensis_contig11.2014.1prot_S-ischiensis_contig11.2014.1Schizocladia ischiensis KU_0333polypeptideS-ischiensis_contig11 447970..450185 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-ischiensis_contig11.2014.1

>prot_S-ischiensis_contig11.2014.1 ID=prot_S-ischiensis_contig11.2014.1|Name=mRNA_S-ischiensis_contig11.2014.1|organism=Schizocladia ischiensis KU_0333|type=polypeptide|length=95bp
VALEDCTKYCESVYTTKRRPTRTVWVGKVPIGSEHRVALQTMTTTDTRDV
DATVEQVIACANAGADLVRITVQGKQEAKACMEIRQKLFDKASQP
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mRNA from alignment at S-ischiensis_contig11:447970..450187+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-ischiensis_contig11.2014.1 ID=mRNA_S-ischiensis_contig11.2014.1|Name=mRNA_S-ischiensis_contig11.2014.1|organism=Schizocladia ischiensis KU_0333|type=mRNA|length=2218bp|location=Sequence derived from alignment at S-ischiensis_contig11:447970..450187+ (Schizocladia ischiensis KU_0333)
GTGGCCTTGGAGGACTGCACCAAGTACTGTGAGTCGGTGTACACCACCAA
GCGCCGACCAACGCGCACGGTGTGGGTGGGCAAGGTCCCCATCGGGTCCG
AGCACCGGGTAGCTCTGCAGACGATGACGACGACCGACACCCGCGATGTC
GACGCCACGGTCGAGCAGGTGCGTCTCACACCGTACATGCATTCCAGTAT
TAGCAAGGACAACACAGGGCGAGGTGCAGGCCACGTCACTCAAGACCTCA
ACAGTTGCAGGTTGAGTGACGCAAGGGAAGCTACTCCAGCGTTTACCTAT
ATATCGCAGCGGACCGAAAAGTTCGGCTTGTCACGTGGCAGCTTGATGGC
GAGGTGCGCGTGTGATTCTCGGTTTCAGGAGTGCGTCTTGTGGCTGAACT
GCCTATGGTATGTGGAGTACAGACAAGGGGAGGACCATGGTTTTACCTGT
TGCCTAGACACAAGATTGGAACATGGCTTAACATGCTACAGTACATCAAG
TACTTTGTATTTCAGGTGTGTTGGCGGTGTTAGGCTTAACCTTGGGAGTG
CCCGGAGCCAACGTGCCGCAGGTGCATGCAGCACATTTTGGCACCAGCGC
TTGGAAGATATTCGTTTGCCCTGTTCAGCAGCGTTGTGAAGCGGCAGCGG
ATAAACTGGAGTGTGTATGCGATAGGCAGCTGGTTTGGGCTGCGCTGGTT
TTCCATTCTTCTGCTTCGAATCTGCCCACGTGTTGGCCTGCAATAAGCCT
GTGCACATCCGCATAGCCGGTGCTGGCTGAAAATGCCTCCCAAGGACACA
CTTCCAAGTGCTTCTAAGTACATAAAAAACGCAACAAAAAAAGGGTTCCA
TCAACATATGGTACAATAGACGTGTGAACATCGGCGACAAATGGCTCATG
GAAGTTGACTGACGATTATTTCGGTTTCGATTCCTGTTTGCGATTTTGTA
AATACTCGTACAAGTTGTATGCACAGAGACGTGAGTGGGTACAAGTAGGA
GGGAAAAAATGGAAAGAACTGGGCTGCTGACCGGCTAGGCTTCAATTCGC
AGGGTTCAATGCTGGCCCAAGCCTCCCCCAAAATCTCAGACAATAGCTCG
GTTGCCAGCTGTGAAACATGGATACAAGAGGGCCACGTAGAGCTGGCGCC
TTGAATGTAACCGGAAGAAAAAAATGATGGCCTAAATACAACCGGTCGCA
CAAGAAATCATGAACCGTCCAGCCAGCTGCCTTGAGGCAACTCTACTTCC
TAGCTAGATGTTGACAACACTCTGGACCATTGCGGGCAAGCATGCCCGGT
GCGTTGAATTCCCTTTTTGAGCTGTGAAGTAGGCAAGGGTTTTGTGTTTT
CTGGTCGCGGCTTTGGTCCACTGCCGAAGCCCTCAACAAAGCCGAGCGGC
GCCTTTTGTTACTTTCCCCCATATACATACTATATATGGCAAGCACAACG
TGAGTACTCTACATATACATATATATTTATATATGTATGCTGGAGGGCAA
ACCCGAAGTTTCGACTTAGGGCTGTCGGCATTGCACGCGCGGTTATGTCG
CAAGATAAGCCAACGAGTACATATATGGCATGCTCCGTCCCGGCCTAGTT
GGGATGCGAGAGTCCATGGCTTAATAGCTGCTCCTGTCGGAGGGCAAACC
TTAAGTTTCGACTTTGGGCTGTCGGCATTGCACGCACGGTTATGTCGCAA
GACAAACCAATGAGTACATATATATATATATATACTCACGTTGTGCTTTC
CGCAGTCCCGAGCCCATTGACCACGTGCCTCGGTCAGACGTATGAAGTAT
ACTCAGAAGTACTCGAGTATATATATACTACTGTACGTAGTATGCACTGC
GTTGTGCTTGCTGTAATCCCGGGACCATTGATCATGCACCGTTGACGACG
GAGCATTCACCCTCCCGGAGCTAGCTGAAAACAGAAACCCAGAAAATTTC
TCGTAGGTGCAATCTCACGTGCGTGGAATTGAGTGAGCTCGTGTAGCTGA
AATGGAGCGGGTAGTTCCGTATAATAGCGATTGATGACAACAGTTCATCT
GATTGTTGGCGCAACAAAAACCATCGCTCGTCCTAATCGCACGGCACAGG
TGATTGCGTGTGCCAACGCCGGGGCAGACCTGGTTCGGATCACGGTGCAG
GGCAAGCAAGAGGCCAAGGCTTGCATGGAGATCCGCCAGAAGCTCTTTGA
CAAGGCGAGCCAGCCAGC
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Coding sequence (CDS) from alignment at S-ischiensis_contig11:447970..450187+

>mRNA_S-ischiensis_contig11.2014.1 ID=mRNA_S-ischiensis_contig11.2014.1|Name=mRNA_S-ischiensis_contig11.2014.1|organism=Schizocladia ischiensis KU_0333|type=CDS|length=570bp|location=Sequence derived from alignment at S-ischiensis_contig11:447970..450187+ (Schizocladia ischiensis KU_0333)
GTGGCCTTGGAGGACTGCACCAAGTACTGTGAGTCGGTGTACACCACCAA
GCGCCGACCAACGCGCACGGTGTGGGTGGGCAAGGTCCCCATCGGGTCCG
AGCACCGGGTAGCTCTGCAGACGATGACGACGACCGACACCCGCGATGTC
GACGCCACGGTCGAGCAGGTGGCCTTGGAGGACTGCACCAAGTACTGTGA
GTCGGTGTACACCACCAAGCGCCGACCAACGCGCACGGTGTGGGTGGGCA
AGGTCCCCATCGGGTCCGAGCACCGGGTAGCTCTGCAGACGATGACGACG
ACCGACACCCGCGATGTCGACGCCACGGTCGAGCAGGTGATTGCGTGTGC
CAACGCCGGGGCAGACCTGGTTCGGATCACGGTGCAGGGCAAGCAAGAGG
CCAAGGCTTGCATGGAGATCCGCCAGAAGCTCTTTGACAAGGCGAGCCAG
CCAGTGATTGCGTGTGCCAACGCCGGGGCAGACCTGGTTCGGATCACGGT
GCAGGGCAAGCAAGAGGCCAAGGCTTGCATGGAGATCCGCCAGAAGCTCT
TTGACAAGGCGAGCCAGCCA
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