mRNA_S-dermatodea_contig909.17169.1 (mRNA) Saccorhiza dermatodea SderLu1190fm monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-dermatodea_contig909.17169.1
Unique NamemRNA_S-dermatodea_contig909.17169.1
TypemRNA
OrganismSaccorhiza dermatodea SderLu1190fm monoicous (Saccorhiza dermatodea SderLu1190fm monoicous)
Homology
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BLAST of mRNA_S-dermatodea_contig909.17169.1 vs. uniprot
Match: D7G864_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G864_ECTSI)

HSP 1 Score: 268 bits (684), Expect = 1.240e-78
Identity = 150/266 (56.39%), Postives = 189/266 (71.05%), Query Frame = 3 
Query:   21 VAGMWDSDASDSSSGHTDNETDMDQSESSDMDLCYDSCCDQPRVTKQPRFPGRPDPDSVVTDSDSSTDVEQPCL---DKLERRGSLEASGPAFYGVWKRTSCEGYEELLRSSGVPRRAAAVALAKHPVHIIDHDGAYFRLIVKNGLSKVDDTFFIGEQ-RVDKIGKENYDVCLNWAHISGTGTKDELTLTSVCRERGEEVIAIRSLEDGGNRMVLTQIVRRPAKQYEVKAKHFFKRVTDKAELRS-------TRRGPTSNVVTVRQ 785
            +A +W+S++S+SS GHTD ETD  ++   D     D CC                  SV     S T+   PC+    +L R  + EA  PAFYGVWKRTS EGYEELL SSGVP+RA A+A+ KHPVHIIDHDG+YFRLIVKNGLSKVD+TFFIG++ + D IGK++Y+V +NWAH+SG G +DEL LTS+C + GEEVIAIRSL +GG++MVLTQIVRRPA+ YEV+AKHFFKRVT K+ELRS        ++G +S +VTVR+
Sbjct:  193 LAKLWESESSESSEGHTDLETDDTET---DWHSDADDCC------------------SVG----SGTEEAPPCVVEQQQLRRGSNSEAGSPAFYGVWKRTSSEGYEELLLSSGVPKRAVAMAMKKHPVHIIDHDGSYFRLIVKNGLSKVDNTFFIGDEPKQDIIGKQSYEVSMNWAHLSGHGREDELALTSICHQAGEEVIAIRSLHEGGDQMVLTQIVRRPAEGYEVRAKHFFKRVTSKSELRSGGKQQHKQQKGLSSTIVTVRR 433
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BLAST of mRNA_S-dermatodea_contig909.17169.1 vs. uniprot
Match: A0A835YHH8_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YHH8_9STRA)

HSP 1 Score: 142 bits (358), Expect = 4.550e-32
Identity = 86/206 (41.75%), Postives = 114/206 (55.34%), Query Frame = 3 
Query:  225 VEQPCLDKLERRGSLEASGPAFYGVWKRTSCEGYEELLRSSGVPRRAAAVALAKHPVHIIDHDGAYFRLIVKNGLSKVDDTFFIGEQ-RVDKIGKENYDVCLNWAHISGTGTKDELTLTSV---CRERGE------------------------------------EVIAIRSLEDGGNRMVLTQIVRRPAKQYEVKAKHFFKRVT 722
            V Q  +  LERR +L    P F G+WKR+  EG+ ELL  SGVPR+AA  A  K PVHIIDHD  YFRLI+KNGL K D  + IG++ R++K G  +Y+V L+W H +  G  + L LTS+    +  G                                     E+IA+R+LEDGG+ +VL QIVR+PA   E +A+H FKR+T
Sbjct:  186 VRQKRMISLERRRALR---PNFSGIWKRSRIEGFIELLAFSGVPRKAALAAAQKRPVHIIDHDDDYFRLIIKNGLVKSDAHYIIGDEPRIEKSGATDYEVVLDWGHGTANGRAESLVLTSISVNAKTGGSDSNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAAIEMIAVRTLEDGGDVLVLQQIVRKPATGEEARARHIFKRIT 388
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BLAST of mRNA_S-dermatodea_contig909.17169.1 vs. uniprot
Match: A0A836CAN9_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CAN9_9STRA)

HSP 1 Score: 114 bits (284), Expect = 7.930e-24
Identity = 71/160 (44.38%), Postives = 90/160 (56.25%), Query Frame = 3 
Query:  246 KLERRGSLEASGPAFYGVWKRTSCEGYEELLRSSGVPRRAAAVALAKHPVHIIDHDGAYFRLIVKNGLSKVDDTFFIGEQRVDKIGKENYDVCLNWAHISGTGTKDELTLTSVCRERGEEVIAIRSLEDGGNRMVLTQIVRRPAKQYEVKAKHFFKRVTD 725
            +L RR +  A+ P F GVWKR   EG+ ELL  SGVPR+AA  A  +  VHIIDHD  YFRLIVKNGL+KVD  + I + +               A  +                   E+IA+R+LEDGG  +VL QIVRRPA   E +A+H FKR+T+
Sbjct:  118 ELRRRAA--AARPDFSGVWKRARVEGFIELLAFSGVPRKAALSAAQRRAVHIIDHDDDYFRLIVKNGLTKVDGHYIIEKLKFXXXXXXXXXXXXIAAAAAPL-----------------EMIAVRTLEDGGATLVLQQIVRRPATGEEARARHIFKRLTE 258
The following BLAST results are available for this feature:
BLAST of mRNA_S-dermatodea_contig909.17169.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7G864_ECTSI1.240e-7856.39Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A835YHH8_9STRA4.550e-3241.75Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CAN9_9STRA7.930e-2444.38Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-dermatodea_contig909contigS-dermatodea_contig909:8129..12486 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Saccorhiza dermatodea SderLu1190fm monoicous2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionCalycin
Ec32 orthologEc-12_001340.1
Exons2
Model size2182
Cds size777
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-dermatodea_contig909.17169.1prot_S-dermatodea_contig909.17169.1Saccorhiza dermatodea SderLu1190fm monoicouspolypeptideS-dermatodea_contig909 9505..12457 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622925385.5838406-UTR-S-dermatodea_contig909:8128..95041622925385.5838406-UTR-S-dermatodea_contig909:8128..9504Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig909 8129..9504 -
1694079898.0604055-UTR-S-dermatodea_contig909:8128..95041694079898.0604055-UTR-S-dermatodea_contig909:8128..9504Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig909 8129..9504 -
1622925385.62379-UTR-S-dermatodea_contig909:12457..124861622925385.62379-UTR-S-dermatodea_contig909:12457..12486Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig909 12458..12486 -
1694079898.0930092-UTR-S-dermatodea_contig909:12457..124861694079898.0930092-UTR-S-dermatodea_contig909:12457..12486Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig909 12458..12486 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622925385.5964766-CDS-S-dermatodea_contig909:9504..98191622925385.5964766-CDS-S-dermatodea_contig909:9504..9819Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig909 9505..9819 -
1694079898.0700464-CDS-S-dermatodea_contig909:9504..98191694079898.0700464-CDS-S-dermatodea_contig909:9504..9819Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig909 9505..9819 -
1622925385.6087441-CDS-S-dermatodea_contig909:11995..124571622925385.6087441-CDS-S-dermatodea_contig909:11995..12457Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig909 11996..12457 -
1694079898.0826747-CDS-S-dermatodea_contig909:11995..124571694079898.0826747-CDS-S-dermatodea_contig909:11995..12457Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig909 11996..12457 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-dermatodea_contig909.17169.1

>prot_S-dermatodea_contig909.17169.1 ID=prot_S-dermatodea_contig909.17169.1|Name=mRNA_S-dermatodea_contig909.17169.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=polypeptide|length=259bp
MWDSDASDSSSGHTDNETDMDQSESSDMDLCYDSCCDQPRVTKQPRFPGR
PDPDSVVTDSDSSTDVEQPCLDKLERRGSLEASGPAFYGVWKRTSCEGYE
ELLRSSGVPRRAAAVALAKHPVHIIDHDGAYFRLIVKNGLSKVDDTFFIG
EQRVDKIGKENYDVCLNWAHISGTGTKDELTLTSVCRERGEEVIAIRSLE
DGGNRMVLTQIVRRPAKQYEVKAKHFFKRVTDKAELRSTRRGPTSNVVTV
RQPRNRAC*
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mRNA from alignment at S-dermatodea_contig909:8129..12486-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-dermatodea_contig909.17169.1 ID=mRNA_S-dermatodea_contig909.17169.1|Name=mRNA_S-dermatodea_contig909.17169.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=mRNA|length=4358bp|location=Sequence derived from alignment at S-dermatodea_contig909:8129..12486- (Saccorhiza dermatodea SderLu1190fm monoicous)
GCTCGGAGCACAAGGCACCGGTCGCGGGCATGTGGGACTCCGACGCCTCC
GACAGCAGCTCCGGCCACACGGACAATGAGACAGACATGGACCAGTCCGA
GTCTTCGGACATGGATTTGTGCTATGACTCGTGCTGTGACCAGCCGCGTG
TGACTAAGCAGCCGCGCTTCCCGGGTCGCCCTGACCCTGACAGCGTGGTG
ACGGATTCGGACTCTTCGACGGACGTGGAGCAGCCATGCCTCGACAAGCT
CGAGCGCCGGGGAAGTTTGGAGGCGTCAGGCCCTGCTTTCTACGGCGTGT
GGAAGCGCACGAGCTGTGAGGGCTACGAGGAGCTGCTGCGATCATCCGGC
GTGCCGAGGAGGGCCGCGGCGGTCGCGCTGGCCAAGCACCCTGTCCACAT
CATCGACCACGATGGGGCCTACTTCCGTCTCATCGTGAAGAACGGCTTGT
CCAAAGTCGACGACACCTTCTTCATCGGCGAACAGAGGGTGGTGAGTGGC
AAGAGACGACGCTGTAGTAGCGTCACGCCGAGCGTGGCAGCCTGCGTACT
GCTATCTGCCGCTGTATTGCACGCTTTTCCCCCTCGCTCGCTTGATCGTG
CGCGCTCGCTTCGCTTGTCCGTTTGGGTTTGATTTGTGTCCTCTTTTGAC
TAGATTTTTTCTCACGTGGGTATATTTACTTGTATACAGCACAGAGACCT
CTATTCTGCCATTTTTTGTTGCTCCGTCTCAAAGTGTTCCTTTGTTTCAC
CGATTTGCCTTTTCCGAGTACAGCAGTATCATATGATAGTATCATGATAT
GGGAACGGGCATGTGTGTTGTACAGTATATCCTGCCAGAAACCCAAGCCG
ACGTCCTTGCCAGCGTCTCTGTTTGCGTGGGCGGCTCCGATGTGCAGTAG
CTCGTTTTCTATGCTCCTCCGGCCTTGGGGATATCGGCTACGCTCGACGT
TGCGAGGCCCTGTTTCAATTTCAACTCTCTTCAAACTTTCGATGGACGAG
GTCCCGCGCACCGCATCTTGAAAACAAAGCCTTCTGTTTGCTGTTTGGTG
GGCAGGTACTGCTGTAAAACAAAACTCAAACTCACGCCAGCACTTGATTG
TTTTCATCTGTCTCTCGTCGTTTTAGCATACTATCCATATATATGAGAGA
GGCTTCGGCGGAGAATGAGAAGTTGACGTGGCCTCTTACATTACCCGCCG
CCTCCACCGCGGTTCATGCTTAGCTCTGCGATATGAGTTGTTACACTCAC
GTGCAGTAAGAACTTCCAAGGACCAGTATGAAATACGAAACGAAGACCTC
GGCTTCAAAAGCAATCCGGTCTGTTCGGATAATTCTACACTCTTGGAGGC
CCCAAGTACGTTGTCTTCGCGTGCTGTTCCACAACGCTTCGGCCGTACAG
ACGTTTGGCAAGCATGCAATCTATGTAGTGATGTGATAACAGCTGTAGCT
GTCGTTGGTTCGATTCACTTTTCTGTACCGCTGCTTTGGTTGTGTCGTTA
TCTGTGTGGTTGTTCTGGGTCGTCCGCTTTGTAAAACGTACTTGTACGTC
CACTGCATGCCGAAAGCAGCCAAGTGTTCGTTGGGATGTATTGACACTAC
TGACAGCAGTTCGACCGAGACTTTTGAGAGAGTTGTATTATTTATGATAG
GTTAACTTGGCCTTCACATTTAATGATACTCACTCTGTACAGCAGTGCTG
CCTTTCCTCGTGTTGAGATGGCGTGTGGTCTTCACTATGATAGCGTGAGG
TGTCAAGCTGACGCGGACTGAGGAGCGGTTGCAAGTACCATATCAGGTGC
CATTTTTCGGTTCCATAGTGCAGAGCTCGATCAAAGCGTGTGCAGTGTTG
GATCAGTTTATGTTCTTCTATGCACATATTTGAACGCATGCTTTTGTAAT
TAACCCGTTCTTCGGTTTATTTCACTCTTTGCTGGGTAGTAATAAAAGCA
GTATAATCTGAGGTGCAAAAAGGCTGTTCAAGTTTACCCAAGGATCAGTA
CGGGTAGTGCCCGAAACAAGCCCAACGGCCACACATGAGGCACGAGGGGA
AGAGTGGTGTATCATACCTACAATGATGAGGAGAGTGACCGTACACCTGA
AAGCGCAACTGAGTGTTGTACCCCCGTGATTCCCCGCGTATTTCCATTAA
AGCCTCCACAACCGGGGCGTTGTATGTATACACACTAGTCCACCTGTACA
CTTATAGTCCCCCCCAGCACATATGCCTTACTGAGAGAGTAAAGGNNNNN
NNNNNCAGTTTCCCCTGAACCAGTGTGCTTGAGAGTTAGGGATGTACATG
GGAACTAGATTTTGTTTTTTATAAACCGGGGCGTGCCCAAATGCAGTTTC
GCTTTCCGGGATCGCGGGCAGGGAGCCGTTTGCTGGCACCTTTGCCTCCT
GTCGGCCGAAGTCGTCGAAGGGAAGAACCTTTTTTCTAGTTATATATATT
CCATGCCTGTGATACATATGAAAACTAGACGTTACGCTTTTCGACGATGA
AAGAAGGTTCCTACGCCGCCATCAGCTGCTGCGCGCATGGCTCGGGAGAA
CGCCGCGTCGCTTGAGCTGGCTCGCATCCGTCTTGTCCTGTCCGCTATTC
TCTCAGAGGCATCGTATCACATCTCCTCTTACCTTACATTTATGTTTGAT
TTTGAACATGCTCGCAGGACAAAATTGGCAAGGAGAACTACGATGTTTGC
CTCAACTGGGCGCACATCTCGGGCACAGGGACGAAGGATGAGCTCACGCT
CACCAGTGTCTGCCGCGAGAGGGGCGAGGAGGTCATCGCGATTCGGTCGC
TCGAGGACGGCGGCAACCGCATGGTGCTCACCCAGATCGTGCGCCGGCCG
GCGAAGCAGTACGAAGTTAAAGCGAAGCACTTCTTCAAGCGCGTCACGGA
CAAGGCAGAGCTGCGCAGCACCCGGAGGGGGCCGACCTCGAACGTGGTGA
CGGTACGGCAGCCCAGAAACAGGGCGTGCTGACGCCGCTCCCACTCCCTA
GTGGATTGACGGATTAACTCTAGTAGTATGATAGTGGTGGTGTGGTAGCG
CGAAACGAGGTGTTTCTTGTCTTCGTTCTCGTTACTCGGTTCGATCGTTA
TGTACTTTTATGGCTGCGGCCGGTCGCTGGGTTATCTCGGCCATAGAGGT
GTTTGTTTTTTTCTCAGATGAGGGGTGGTCACGAGCGTCACGCAACAATG
TTGGCGATGCGATGTGAAGTAAAATAAATAGCAGTTGGCCGCTCTGCGTA
GTGTGTGCTTGACGCGTACAAGCTTTGGCGTTGTTCGCGAACGAGAATAA
TTTAGTTGGCAAGAACCGAAATTTTGCTCGGCTTGGAAGGCCTTCGCACT
GGCGGAGACGCTATGTACAGATTCAGACACCACGAGCCGGCGCTGCTGCT
TGCAAAGCGCGTTTAGAGGATTGTGCTGTGTTGTGTATGGTTAGGTTTGG
CTGTTCCGTTTCACGACGAATCAGTACCCGATAAATGTGTCGTATCCTAA
AGCAGTGGGTTTTTTTAGTTGCTTCCTGCTCTTTTGTTCAGCGATATAAA
CGTGAAGTTGACGGTTTTATTCACCGTCTTGGACGAAAGGCTCACGGGAT
GCGGAGCGCGTCCATTTTGAGCTGCTATCTCGCCCGCGTTGGGCGTTGAG
TTGTGGGGGTGGTGCGCACTCGGATTGTCGCTGGCTCGGCGAGGCACAGT
CCGAAGAGTTTGTCGTTGTAGGAGGCGGTGACTCGTCATGTTTTTTTTTC
CCGAGCCGGTTTTGCAATTCTCCATTGACTTATGATACTATTATTCTTCG
AAGTATATAGTTATCTTGTCTGTCTGTATTTCATGTTTTCTCGCGCGGCA
CCTCAAGACGCGTGGCAATTGGCAATTACTGAAACTCGACAATTTGATGA
GGTCATGGGGTTGGGTTCTGCGTCGGTTGGACGCTTGCAGCAGAATTTTA
GGGTGGTATTTCGGGATTTGGGTTGGAGGGATCCCTTGGGACCTTGTACC
GATATTTTATGATTAATCGTTTGTCGTCCGTTAACAGCACCAGTACATTA
CACATATATACGATATGATAGCTTGTCCTGCAGAAGTCCGAATTTGTTTT
TCATTGTGAGCGTGTTGCAGCCACAGACGGCATGGTGTGGTGTAACTTCT
GCATTTATCTGTCGGACCTGCGCCGGGTTGCATCTGTTGAACGACCTGCG
TAACGCCGTGGCGGTTTTTGGGGGTGATCGTGATCGTGGTGCGTCGTATT
TGGTCGCATTTATTGTGGACTTTAGCTTATCTGCTTACAATACGATTAGA
AGACGGGCAAGAACCATCGTGTCATTGCAACGAACGTTCGTTTCGAACAG
TTTTCCAC
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Coding sequence (CDS) from alignment at S-dermatodea_contig909:8129..12486-

>mRNA_S-dermatodea_contig909.17169.1 ID=mRNA_S-dermatodea_contig909.17169.1|Name=mRNA_S-dermatodea_contig909.17169.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=CDS|length=1554bp|location=Sequence derived from alignment at S-dermatodea_contig909:8129..12486- (Saccorhiza dermatodea SderLu1190fm monoicous)
ATGTGGGACTCCGACGCCTCCGACAGCAGCTCCGGCCACACGGACAATGA
GACAGACATGGACCAGTCCGAGTCTTCGGACATGGATTTGTGCTATGACT
CGTGCTGTGACCAGCCGCGTGTGACTAAGCAGCCGCGCTTCCCGGGTCGC
CCTGACCCTGACAGCGTGGTGACGGATTCGGACTCTTCGACGGACGTGGA
GCAGCCATGCCTCGACAAGCTCGAGCGCCGGGGAAGTTTGGAGGCGTCAG
GCCCTGCTTTCTACGGCGTGTGGAAGCGCACGAGCTGTGAGGGCTACGAG
GAGCTGCTGCGATCATCCGGCGTGCCGAGGAGGGCCGCGGCGGTCGCGCT
GGCCAAGCACCCTGTCCACATCATCGACCACGATGGGGCCTACTTCCGTC
TCATCGTGAAGAACGGCTTGTCCAAAGTCGACGACACCTTCTTCATCGGC
GAACAGAGGGTGATGTGGGACTCCGACGCCTCCGACAGCAGCTCCGGCCA
CACGGACAATGAGACAGACATGGACCAGTCCGAGTCTTCGGACATGGATT
TGTGCTATGACTCGTGCTGTGACCAGCCGCGTGTGACTAAGCAGCCGCGC
TTCCCGGGTCGCCCTGACCCTGACAGCGTGGTGACGGATTCGGACTCTTC
GACGGACGTGGAGCAGCCATGCCTCGACAAGCTCGAGCGCCGGGGAAGTT
TGGAGGCGTCAGGCCCTGCTTTCTACGGCGTGTGGAAGCGCACGAGCTGT
GAGGGCTACGAGGAGCTGCTGCGATCATCCGGCGTGCCGAGGAGGGCCGC
GGCGGTCGCGCTGGCCAAGCACCCTGTCCACATCATCGACCACGATGGGG
CCTACTTCCGTCTCATCGTGAAGAACGGCTTGTCCAAAGTCGACGACACC
TTCTTCATCGGCGAACAGAGGGTGGACAAAATTGGCAAGGAGAACTACGA
TGTTTGCCTCAACTGGGCGCACATCTCGGGCACAGGGACGAAGGATGAGC
TCACGCTCACCAGTGTCTGCCGCGAGAGGGGCGAGGAGGTCATCGCGATT
CGGTCGCTCGAGGACGGCGGCAACCGCATGGTGCTCACCCAGATCGTGCG
CCGGCCGGCGAAGCAGTACGAAGTTAAAGCGAAGCACTTCTTCAAGCGCG
TCACGGACAAGGCAGAGCTGCGCAGCACCCGGAGGGGGCCGACCTCGAAC
GTGGTGACGGTACGGCAGCCCAGAAACAGGGCGTGCTGAGACAAAATTGG
CAAGGAGAACTACGATGTTTGCCTCAACTGGGCGCACATCTCGGGCACAG
GGACGAAGGATGAGCTCACGCTCACCAGTGTCTGCCGCGAGAGGGGCGAG
GAGGTCATCGCGATTCGGTCGCTCGAGGACGGCGGCAACCGCATGGTGCT
CACCCAGATCGTGCGCCGGCCGGCGAAGCAGTACGAAGTTAAAGCGAAGC
ACTTCTTCAAGCGCGTCACGGACAAGGCAGAGCTGCGCAGCACCCGGAGG
GGGCCGACCTCGAACGTGGTGACGGTACGGCAGCCCAGAAACAGGGCGTG
CTGA
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