mRNA_S-dermatodea_contig8921.17014.1 (mRNA) Saccorhiza dermatodea SderLu1190fm monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-dermatodea_contig8921.17014.1
Unique NamemRNA_S-dermatodea_contig8921.17014.1
TypemRNA
OrganismSaccorhiza dermatodea SderLu1190fm monoicous (Saccorhiza dermatodea SderLu1190fm monoicous)
Homology
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A6H5JTL8_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTL8_9PHAE)

HSP 1 Score: 127 bits (319), Expect = 1.010e-31
Identity = 78/125 (62.40%), Postives = 87/125 (69.60%), Query Frame = 1 
Query:    1 QVTPIVGNRSVESS---------PSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            QVTPIVG+RSV+S+         P H  S    DQLPFEP+ P+ VSAM P  LEAHLT AYNAL   SA VRQRGQTL+YLQSLAP+PRVANLLVNSTFL+LLLR           +TVA LIG
Sbjct: 1233 QVTPIVGSRSVDSAGFPLAAQHQPRHPFSPPRFDQLPFEPLLPAAVSAMPPPQLEAHLTVAYNALAGGSAPVRQRGQTLVYLQSLAPIPRVANLLVNSTFLVLLLR-----------DTVAALIG 1346
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A7R9U667_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U667_9STRA)

HSP 1 Score: 68.6 bits (166), Expect = 3.960e-11
Identity = 41/116 (35.34%), Postives = 68/116 (58.62%), Query Frame = 1 
Query:    1 QVTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            QV+PIV N  +E S    R +      PFEP+  +++  + P+ LE  LTE Y +L   S  +++R + + YL+ ++  P  A++++NSTFL LLLR LR+ +  A +  V + +G
Sbjct:  193 QVSPIVQNPGIEKS---ARPKVNTRSFPFEPLPAASLEKLPPNKLEDFLTEVYRSLATNSLDMQERLRIITYLEVISASPNAAHIIMNSTFLTLLLRFLRRLKTPAAKARVLSCLG 305
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A7S1U449_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U449_9STRA)

HSP 1 Score: 62.4 bits (150), Expect = 2.900e-9
Identity = 43/116 (37.07%), Postives = 64/116 (55.17%), Query Frame = 1 
Query:    1 QVTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            QV+PIV N+ +E     +  + +   LPF   +   + AM  S LEA LT  Y +L  ++     R   + YLQ +A  P  AN+++NSTFL LLLRLLR+ + A  +  V + +G
Sbjct:   84 QVSPIVLNKDIERLAPPKVKEGS---LPFRARAVGDLEAMDSSELEAFLTSVYRSLGGSAVTTNDRLGVVAYLQVVAASPNAANVVLNSTFLPLLLRLLRRLKTAPAKARVVSCLG 196
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A1V9ZE49_9STRA (Serine/threonine protein kinase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZE49_9STRA)

HSP 1 Score: 59.3 bits (142), Expect = 7.410e-8
Identity = 42/116 (36.21%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:    4 VTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLTEAYNALT-EASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            V PIVG+ ++E   + R  +AA   LP EP++ +T        +EAHL + Y+ L  +ASAA  ++  TL YL S+A  P++AN++VNS+ + LL++LL +   +A+   +  ++G
Sbjct:  374 VVPIVGSNNIEVVSTPRYKEAA---LPVEPLTTNT-------DIEAHLEKIYHFLRGDASAA--EKHNTLAYLCSIAAFPKLANVIVNSSLMTLLVKLLERSTSSALSTRLCLVLG 477
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A5J4XC59_9CHLO (Serine threonine-kinase ULK4-like (Fragment) n=2 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4XC59_9CHLO)

HSP 1 Score: 59.3 bits (142), Expect = 7.530e-8
Identity = 39/115 (33.91%), Postives = 64/115 (55.65%), Query Frame = 1 
Query:    4 VTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            V PIV NR +E  P  +    A   LPF+P++   + + +   LEA LT+ Y A+  A A ++ +   L Y ++L      AN+L+NS+  IL++R+LR  R   +R  +A+ +G
Sbjct:  476 VKPIVANRRIERLPEPKWDAQA---LPFKPLALQDMLSASQQDLEAFLTQVYRAIAGA-APLKDKVNVLAYFETLCTDTGAANVLINSSLTILIVRMLRNARAPTLRVRLASALG 586
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A078B1X2_STYLE (Serine threonine-protein kinase ulk4-like n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078B1X2_STYLE)

HSP 1 Score: 57.4 bits (137), Expect = 3.530e-7
Identity = 40/116 (34.48%), Postives = 64/116 (55.17%), Query Frame = 1 
Query:    4 VTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSA-MTPSGLEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            V PI+GN+ +E             QLPFE +S   V+A +  S +E+HL E YNA+   S  V  +   L Y +S+     VAN+L+NS F+ LLL+L++  +   ++  + ++IG
Sbjct:  311 VKPIIGNKEIEKI---GELVFNGQQLPFEALSVDQVAATINSSTIESHLHEVYNAMAS-SGTVNDKINVLCYFESIILNSNVANILINSAFMGLLLKLMKNVKSPHLKIRLCSIIG 422
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: J9EWA0_9SPIT (Serine/threonine protein kinase n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9EWA0_9SPIT)

HSP 1 Score: 55.5 bits (132), Expect = 1.680e-6
Identity = 40/118 (33.90%), Postives = 65/118 (55.08%), Query Frame = 1 
Query:    4 VTPIVGNRSVESSPSHRRSQAA--RDQLPFEPISPSTVSAMTPSG-LEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            V PI+GN+ +E     + S+      QLPFE +S   V+A   S  +E+HL E YNA+   S  V  +   L Y +S+     VAN L+NS F+ LLL+L++  +   ++  + +++G
Sbjct:  485 VKPIIGNKEIE-----KISEIVFNAQQLPFEAMSVDQVAATIESASIESHLHEVYNAIAS-SGTVNDKINVLCYFESIILNSNVANRLINSAFMGLLLKLMKNVKSPHLKIRLCSIVG 596
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BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Match: A0A8J1ZM33_9DINO (Hypothetical protein n=1 Tax=Amoebophrya sp. A120 TaxID=2202890 RepID=A0A8J1ZM33_9DINO)

HSP 1 Score: 50.8 bits (120), Expect = 7.020e-5
Identity = 39/115 (33.91%), Postives = 65/115 (56.52%), Query Frame = 1 
Query:    4 VTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLTEAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLRKPRPAAIRETVATLIG 348
            V PI  N ++E   S   SQ   + LPF P++   V   +   LE+ LT+ Y  + + +  +  +  TL+YL++L    +VA+L+VNS+ L L+L+++R  R   +R  + TLIG
Sbjct:  694 VRPISNNPAIEKITSPHLSQP--ELLPFRPLALEDVFVCSNQELESFLTKVYKCIAQGT--IETKIMTLIYLENLCGHMQVADLVVNSSLLKLVLKMVRV-RSTELRVRLWTLIG 803
The following BLAST results are available for this feature:
BLAST of mRNA_S-dermatodea_contig8921.17014.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
A0A6H5JTL8_9PHAE1.010e-3162.40Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A7R9U667_9STRA3.960e-1135.34Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
A0A7S1U449_9STRA2.900e-937.07Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A1V9ZE49_9STRA7.410e-836.21Serine/threonine protein kinase n=1 Tax=Thraustoth... [more]
A0A5J4XC59_9CHLO7.530e-833.91Serine threonine-kinase ULK4-like (Fragment) n=2 T... [more]
A0A078B1X2_STYLE3.530e-734.48Serine threonine-protein kinase ulk4-like n=1 Tax=... [more]
J9EWA0_9SPIT1.680e-633.90Serine/threonine protein kinase n=1 Tax=Oxytricha ... [more]
A0A8J1ZM33_9DINO7.020e-533.91Hypothetical protein n=1 Tax=Amoebophrya sp. A120 ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-dermatodea_contig8921contigS-dermatodea_contig8921:6214..8649 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Saccorhiza dermatodea SderLu1190fm monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score60.8
Seed ortholog evalue4.7e-07
Seed eggNOG ortholog3067.XP_002945637.1
KEGG koko:K17545
GOsGO:0000003,GO:0000226,GO:0000278,GO:0000281,GO:0000910,GO:0000911,GO:0000912,GO:0000914,GO:0001932,GO:0003006,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005819,GO:0005856,GO:0005911,GO:0006996,GO:0007010,GO:0007017,GO:0007049,GO:0007275,GO:0007349,GO:0007399,GO:0008017,GO:0008092,GO:0008150,GO:0009506,GO:0009524,GO:0009555,GO:0009556,GO:0009574,GO:0009653,GO:0009966,GO:0009987,GO:0010245,GO:0010342,GO:0010646,GO:0010975,GO:0015630,GO:0015631,GO:0016043,GO:0019220,GO:0019222,GO:0019953,GO:0022008,GO:0022402,GO:0022414,GO:0022607,GO:0023051,GO:0030054,GO:0030154,GO:0030334,GO:0031323,GO:0031344,GO:0031399,GO:0032268,GO:0032501,GO:0032502,GO:0032506,GO:0032872,GO:0032879,GO:0034293,GO:0040012,GO:0042325,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043408,GO:0043934,GO:0044085,GO:0044422,GO:0044424,GO:0044430,GO:0044444,GO:0044446,GO:0044464,GO:0044703,GO:0045595,GO:0045664,GO:0046328,GO:0048229,GO:0048236,GO:0048583,GO:0048646,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050789,GO:0050793,GO:0050794,GO:0051128,GO:0051171,GO:0051174,GO:0051239,GO:0051246,GO:0051270,GO:0051301,GO:0051321,GO:0051704,GO:0051960,GO:0055044,GO:0060255,GO:0060284,GO:0061640,GO:0065007,GO:0070302,GO:0071840,GO:0080090,GO:0080134,GO:0080135,GO:0090036,GO:0120035,GO:1900744,GO:1902407,GO:1902410,GO:1902531,GO:1903046,GO:1903047,GO:2000026,GO:2000145,GO:2001222
EggNOG free text desc.Serine/Threonine protein kinases, catalytic domain
EggNOG OGs34GZ6@3041,37I7E@33090,KOG0597@1,KOG0597@2759
EC2.7.11.1
COG Functional cat.T
Best tax levelChlorophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01001
Hectar predicted targeting categoryother localisation
Exons2
Model size349
Cds size348
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622925373.1452477-CDS-S-dermatodea_contig8921:6214..62721622925373.1452477-CDS-S-dermatodea_contig8921:6214..6272Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig8921 6215..6272 -
1694079889.3267038-CDS-S-dermatodea_contig8921:6214..62721694079889.3267038-CDS-S-dermatodea_contig8921:6214..6272Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig8921 6215..6272 -
1622925373.1600215-CDS-S-dermatodea_contig8921:8359..86491622925373.1600215-CDS-S-dermatodea_contig8921:8359..8649Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig8921 8360..8649 -
1694079889.3393333-CDS-S-dermatodea_contig8921:8359..86491694079889.3393333-CDS-S-dermatodea_contig8921:8359..8649Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig8921 8360..8649 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-dermatodea_contig8921.17014.1prot_S-dermatodea_contig8921.17014.1Saccorhiza dermatodea SderLu1190fm monoicouspolypeptideS-dermatodea_contig8921 6215..8649 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-dermatodea_contig8921.17014.1

>prot_S-dermatodea_contig8921.17014.1 ID=prot_S-dermatodea_contig8921.17014.1|Name=mRNA_S-dermatodea_contig8921.17014.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=polypeptide|length=116bp
QVTPIVGNRSVESSPSHRRSQAARDQLPFEPISPSTVSAMTPSGLEAHLT
EAYNALTEASAAVRQRGQTLLYLQSLAPVPRVANLLVNSTFLILLLRLLR
KPRPAAIRETVATLIG
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mRNA from alignment at S-dermatodea_contig8921:6214..8649-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-dermatodea_contig8921.17014.1 ID=mRNA_S-dermatodea_contig8921.17014.1|Name=mRNA_S-dermatodea_contig8921.17014.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=mRNA|length=2436bp|location=Sequence derived from alignment at S-dermatodea_contig8921:6214..8649- (Saccorhiza dermatodea SderLu1190fm monoicous)
CAGGTGACCCCTATCGTAGGCAACAGGTCCGTCGAGTCTTCGCCTTCCCA
CCGGCGGTCTCAGGCCGCTCGAGACCAGCTCCCCTTCGAGCCGATATCAC
CTTCCACAGTCAGCGCGATGACCCCCTCGGGCCTCGAGGCTCACCTGACC
GAGGCCTACAACGCGCTAACGGAGGCCTCGGCTGCCGTCCGGCAGCGGGG
CCAGACCCTCCTGTATCTGCAGTCGCTGGCGCCGGTGCCGCGAGTCGCTA
ACCTGCTCGTCAACTCCACTTTCCTGATCTTGCTGCTGAGGTGATGCGTG
ATGCGCGATTGAGGGGGGTTGTACTTGTGTTGAACGCATGACGTATCCAT
ACGGACATAGACGATGCGTCCCAACAATGCCGGGAAGGAGCGATATGCCG
GGTTTTCGCCGGCCAGGCTGCGTTTCACAAGGATGATACCGGCTGCTGGT
CATCATTTTTGCGTTTGCGACGCCAACCGATATGCCATGGGTTGATTGGA
CATACTGGGTAACAAAATGTTACACATTCAGTAGGCGTGGCTGTACTCCA
AGCACCGCAAAGGGACTTTGATCTCCGTGCGAATGCAAAAGCACCGCATG
CACACAATATACACGACGGTAAACGTGTGATACACTCGGACCATAATCTG
TTCATGAGTACTCCTGGCGGAGTACTACTCATAAGTCAATGGTTGCTCTA
GGGCGTTCCAATAATCCAAGTTCAGCTGGTGTCCTACTTCGAAGTATAGG
ATGTGATGCGTTTCGCCCCTTTGGGGAGAGACAAGACTCTTCGACCGAGG
TCTCCGAGCTCACAGGACATACTACATGAATCACCCCATGCCCTATCAAT
AGCAGTTTTATAGCTCCATGCCACTAGAGCCAACCCTACCTCGACCAACC
CCACCTCGGACATCAAAGGCAGGGGGGTTGTAGGTCTGAGTTTAACGGGG
GGGGGGCAGAGTTATACTGCCAAGATATCGACCAGACGGAGACATATGCA
CAACGATCCATAGCATTTATTCGTAACATAAGACCCTAGATAGGATCATA
CATAGTAACGCAAGCCTCCATGGGTAGAGGTTGGACCATATTCCTTACCT
CATGCAGGTGGGACCCCAACGCTCGCTCGATGTTTTTTTAAATCAGTTTA
ATGTGTTAGGGGGGGGCGCGGGGGGGGTGTTTCTACGATGGTAGCCTTAT
GTGTAAGTCGGGCTTTTGTGTATACTGGGGGGGGGGCTGAGCCATCCTTG
CACACCAGTAAACAGGTGGATCCCACGGCTCACTTTATGTATTAAAAATC
GATATTTCCTATCATATGGGAGGCTGTGCGTGTGAGGGGGGGGGGGGGGG
GGGGGGGGGGCGTGGGGGGGGGGGGGGGGGGGAGGGACAGAAGTTTTTTT
TTTTGGGGGGGGGGGTGGGGGGTGGAAGTGGGGGTGTGTTGTTGNNNNNN
NNNNGAAGGATGGGGGGTAGACGGACATGCACTGAGGGTGGAGCGATGTA
GGACGGCGTAGTTGGCGATGTGTTTCTGTGATTGTGGGGGGGGTGTGGGG
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGCCTGTTAAGGTATCGAGCG
GGTAAGGCCAAGAGCTACATTTTTCAGGGGTGTTCGGCGTGACCGATGTT
AGGATTCCAGGGCTCATTTTGTCGTTTTGGTGTCGTTCGTGCCGTTGGGA
GGGGAGCGAGGCCAGCATGCGGGCAGGGGCAGTATGCCCTTGTATTAGCT
CGCTCTCCATCTTGCTTTCTCGTTCGCGTCCATTTTACCTGTCTGGTTTT
TCTCGGTTCCGCAGCCCTGGGCACTGTGCTCCCACCCCCCGTTTCGTATG
CAACCGCTGCAGTACAATGGACGCCCTCCTGCCAGCCAGCCGCTCAACGT
CCGCGTTCGGCCGATTATTTCTTTACCAGGCCATTCTCGTAAAGCCCCCC
GCACGCCCACCCAGCTCGCACAGACAGCACCATGAACCCTACTGCCATTT
ACCCCCCAATCGCCTTGGATGTATGACAAGGAGAGATTATTGTGTATAAC
CCAAGTGGGGAAAATATAGAGGTTGTGATAGATACGCTTTGAAAGCATTG
GAAAGCCAGAGGGAGGCATCTTGTAGCAAAACTGGGAGATCAGAAGTGGC
CCGAAAAGTCGACACTTGAAGTAGAGATAACAGGAAAATACTTTTTTGGG
CCACCGTTGGCAACAGTAGGAAGGGACAATTGGCCATTGGCGATCGATCC
TGTGCGAACAATGATCCGCCCCCCCGAGGACGCGTGGTTAATGGCTCAAC
ACCGAAGGCACGCAAAACTTGAGTACCCCCCCCCCACCACCCCCTCGTCC
GTGCTCTACCGCCGTGCCTACACCAAGACTGCTGCGTAAGCCGCGCCCAG
CGGCGATCAGGGAAACGGTGGCGACGCTAATCGGGC
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Coding sequence (CDS) from alignment at S-dermatodea_contig8921:6214..8649-

>mRNA_S-dermatodea_contig8921.17014.1 ID=mRNA_S-dermatodea_contig8921.17014.1|Name=mRNA_S-dermatodea_contig8921.17014.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=CDS|length=696bp|location=Sequence derived from alignment at S-dermatodea_contig8921:6214..8649- (Saccorhiza dermatodea SderLu1190fm monoicous)
CAGGTGACCCCTATCGTAGGCAACAGGTCCGTCGAGTCTTCGCCTTCCCA
CCGGCGGTCTCAGGCCGCTCGAGACCAGCTCCCCTTCGAGCCGATATCAC
CTTCCACAGTCAGCGCGATGACCCCCTCGGGCCTCGAGGCTCACCTGACC
GAGGCCTACAACGCGCTAACGGAGGCCTCGGCTGCCGTCCGGCAGCGGGG
CCAGACCCTCCTGTATCTGCAGTCGCTGGCGCCGGTGCCGCGAGTCGCTA
ACCTGCTCGTCAACTCCACTTTCCTGATCTTGCTGCTGAGCAGGTGACCC
CTATCGTAGGCAACAGGTCCGTCGAGTCTTCGCCTTCCCACCGGCGGTCT
CAGGCCGCTCGAGACCAGCTCCCCTTCGAGCCGATATCACCTTCCACAGT
CAGCGCGATGACCCCCTCGGGCCTCGAGGCTCACCTGACCGAGGCCTACA
ACGCGCTAACGGAGGCCTCGGCTGCCGTCCGGCAGCGGGGCCAGACCCTC
CTGTATCTGCAGTCGCTGGCGCCGGTGCCGCGAGTCGCTAACCTGCTCGT
CAACTCCACTTTCCTGATCTTGCTGCTGAGACTGCTGCGTAAGCCGCGCC
CAGCGGCGATCAGGGAAACGGTGGCGACGCTAATCGGGACTGCTGCGTAA
GCCGCGCCCAGCGGCGATCAGGGAAACGGTGGCGACGCTAATCGGG
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