mRNA_S-dermatodea_contig10097.64.1 (mRNA) Saccorhiza dermatodea SderLu1190fm monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-dermatodea_contig10097.64.1
Unique NamemRNA_S-dermatodea_contig10097.64.1
TypemRNA
OrganismSaccorhiza dermatodea SderLu1190fm monoicous (Saccorhiza dermatodea SderLu1190fm monoicous)
Homology
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BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Match: D7FPM8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPM8_ECTSI)

HSP 1 Score: 243 bits (620), Expect = 9.040e-72
Identity = 116/134 (86.57%), Postives = 126/134 (94.03%), Query Frame = 2 
Query:  329 RAFSSYDWKNMSKYAQEANKEFSKLTPEEVRKAGEDEVDKVEKALKAMVPFNDVFTVRREGLEVFAELGPQVGTYWIKFDLSIQQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPEF 730
            RA SSY+WKNMS  AQ+ANKEF +L+ EE+RKAGE+EVDKVEKALKAM PFNDVF VRREGLEVFAELGP+VGTYWIKFDLSI QIC+MSPRSGPHRYI+DKGSGRWIGEADKHDFQGMLTRDIIQSACGIP+F
Sbjct:  102 RAMSSYEWKNMSAQAQKANKEFGELSREEIRKAGEEEVDKVEKALKAMEPFNDVFEVRREGLEVFAELGPEVGTYWIKFDLSIYQICLMSPRSGPHRYIFDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPDF 235
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BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Match: A0A835Z714_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z714_9STRA)

HSP 1 Score: 92.0 bits (227), Expect = 1.340e-18
Identity = 42/98 (42.86%), Postives = 64/98 (65.31%), Query Frame = 2 
Query:  437 EVDKVEKALKAMVPFNDVFTVRREGLEVFAELGPQVGTYWIKFDLSIQQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPEF 730
            E+++V  A+  M   N +F V     +V  +LGP++GTY I+F   ++ + + SPRSG   Y+YD+ + +W+G  D HDF+GMLTRD+IQ A G P+F
Sbjct:   13 EMERVVAAVDRMKEDNAIFEVIHADGKVSIDLGPKIGTYQIRFVHDLRVLQLSSPRSGGFMYVYDEKTRKWLGREDAHDFEGMLTRDLIQQANGYPDF 110
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BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Match: A0A067C8U5_SAPPC (Uncharacterized protein n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067C8U5_SAPPC)

HSP 1 Score: 67.4 bits (163), Expect = 1.330e-9
Identity = 41/115 (35.65%), Postives = 66/115 (57.39%), Query Frame = 2 
Query:  401 LTPEEVRKAGEDEVDKVEKALKAMVP-FNDVFTVRREGLEVFAELGPQVGTYWIKFDLSI----QQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPEF 730
            LTP++ +      +DKVE A+  ++P  N+ F V+R       +L  Q  T+  +F++++    Q I   SP SG   YIY+  + RW  E D+HD +G+LTRD++++  GIP F
Sbjct:   32 LTPDKFKSLSLAFLDKVEAAVAPLLPPVNETFVVQRTATP--PKLVLQTATH--EFEIAVLAKPQTIEFTSPISGLRTYIYNGKTQRWEDETDRHDVEGLLTRDLMRTCTGIPTF 142
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BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Match: A0A225UL20_9STRA (Uncharacterized protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225UL20_9STRA)

HSP 1 Score: 63.9 bits (154), Expect = 5.510e-9
Identity = 35/85 (41.18%), Postives = 47/85 (55.29%), Query Frame = 2 
Query:  476 PFNDVFTVRREGLEVFAELGPQVGTYWIKFDLSIQQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPEF 730
            P ND F ++R   EV      +   + IK   S QQI   SP SG H Y ++  + RW  EAD HD +G+LTRD+++   GIP F
Sbjct:    7 PINDDFQLQRVDAEVVIRTNTK--EFVIKVLPSKQQIEFSSPVSGLHTYQWNAMAKRWEDEADSHDIEGLLTRDLMRFCAGIPLF 89
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BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Match: A0A0N7L4E5_PLAHL (Mitochondrial matrix protein frataxin n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0N7L4E5_PLAHL)

HSP 1 Score: 65.5 bits (158), Expect = 1.100e-8
Identity = 35/98 (35.71%), Postives = 57/98 (58.16%), Query Frame = 2 
Query:  440 VDKVEKALKAMVP-FNDVFTVRREGLEVFAELGPQVGTYWIKFDLSIQQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPEF 730
            +D+V+ A++ + P  ND F ++R+  E++     +   + +K   S QQI   SP SG   Y ++  + RW  EAD HD +G+LTRD+++   GIP F
Sbjct:   72 LDRVQNAMEPLYPPINDEFQLKRDDGEIYIRTNAR--EFVVKVLHSKQQIEFSSPVSGLRSYQWNVLTKRWEDEADSHDIEGLLTRDLMRFCVGIPRF 167
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BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Match: H3H0V7_PHYRM (WW domain-containing protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3H0V7_PHYRM)

HSP 1 Score: 62.4 bits (150), Expect = 1.870e-6
Identity = 39/100 (39.00%), Postives = 58/100 (58.00%), Query Frame = 2 
Query:  440 VDKVEKALKAM-VPFNDVFTVRREGLEVFAELGPQVGT--YWIKFDLSIQQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQSACGIPEF 730
            +D+V+ A++ +  P N+ F ++REG     EL  +  T  + IK   S QQI   SP SG   Y ++  + RW  EAD HD +G+LTRD+++   GIP F
Sbjct:  351 LDRVQTAMEPLHPPINEEFQLQREGN---GELVIRTNTKEFVIKVLSSKQQIEFSSPVSGLRTYQWNAITKRWEDEADSHDIEGLLTRDLMRFCAGIPLF 447
The following BLAST results are available for this feature:
BLAST of mRNA_S-dermatodea_contig10097.64.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FPM8_ECTSI9.040e-7286.57Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835Z714_9STRA1.340e-1842.86Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A067C8U5_SAPPC1.330e-935.65Uncharacterized protein n=2 Tax=Saprolegnia TaxID=... [more]
A0A225UL20_9STRA5.510e-941.18Uncharacterized protein n=1 Tax=Phytophthora megak... [more]
A0A0N7L4E5_PLAHL1.100e-835.71Mitochondrial matrix protein frataxin n=1 Tax=Plas... [more]
H3H0V7_PHYRM1.870e-639.00WW domain-containing protein n=1 Tax=Phytophthora ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-dermatodea_contig10097contigS-dermatodea_contig10097:4801..8274 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Saccorhiza dermatodea SderLu1190fm monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score245.7
Seed ortholog evalue1.9e-62
Seed eggNOG ortholog2880.D7FPM8
EggNOG OGs2C6YH@1,2S319@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categorymitochondrion
Exons3
Model size2253
Cds size627
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-dermatodea_contig10097.64.1prot_S-dermatodea_contig10097.64.1Saccorhiza dermatodea SderLu1190fm monoicouspolypeptideS-dermatodea_contig10097 4907..6754 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622924138.6905274-UTR-S-dermatodea_contig10097:4800..49061622924138.6905274-UTR-S-dermatodea_contig10097:4800..4906Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig10097 4801..4906 +
1694079043.8081472-UTR-S-dermatodea_contig10097:4800..49061694079043.8081472-UTR-S-dermatodea_contig10097:4800..4906Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig10097 4801..4906 +
1622924138.736067-UTR-S-dermatodea_contig10097:6754..82741622924138.736067-UTR-S-dermatodea_contig10097:6754..8274Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig10097 6755..8274 +
1694079043.846528-UTR-S-dermatodea_contig10097:6754..82741694079043.846528-UTR-S-dermatodea_contig10097:6754..8274Saccorhiza dermatodea SderLu1190fm monoicousUTRS-dermatodea_contig10097 6755..8274 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622924138.709259-CDS-S-dermatodea_contig10097:4906..49431622924138.709259-CDS-S-dermatodea_contig10097:4906..4943Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig10097 4907..4943 +
1694079043.819018-CDS-S-dermatodea_contig10097:4906..49431694079043.819018-CDS-S-dermatodea_contig10097:4906..4943Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig10097 4907..4943 +
1622924138.718933-CDS-S-dermatodea_contig10097:5835..63341622924138.718933-CDS-S-dermatodea_contig10097:5835..6334Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig10097 5836..6334 +
1694079043.8283944-CDS-S-dermatodea_contig10097:5835..63341694079043.8283944-CDS-S-dermatodea_contig10097:5835..6334Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig10097 5836..6334 +
1622924138.7276998-CDS-S-dermatodea_contig10097:6663..67541622924138.7276998-CDS-S-dermatodea_contig10097:6663..6754Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig10097 6664..6754 +
1694079043.8372967-CDS-S-dermatodea_contig10097:6663..67541694079043.8372967-CDS-S-dermatodea_contig10097:6663..6754Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig10097 6664..6754 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-dermatodea_contig10097.64.1

>prot_S-dermatodea_contig10097.64.1 ID=prot_S-dermatodea_contig10097.64.1|Name=mRNA_S-dermatodea_contig10097.64.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=polypeptide|length=209bp
MISCCAKSGVFRGATQRWLLPSCTDLLRKGLAPCSSVPSSSRYRLSTVAT
PLARRRPYSRPSDAIISQTTLSRRRAFSSYDWKNMSKYAQEANKEFSKLT
PEEVRKAGEDEVDKVEKALKAMVPFNDVFTVRREGLEVFAELGPQVGTYW
IKFDLSIQQICMMSPRSGPHRYIYDKGSGRWIGEADKHDFQGMLTRDIIQ
SACGIPEF*
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mRNA from alignment at S-dermatodea_contig10097:4801..8274+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-dermatodea_contig10097.64.1 ID=mRNA_S-dermatodea_contig10097.64.1|Name=mRNA_S-dermatodea_contig10097.64.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=mRNA|length=3474bp|location=Sequence derived from alignment at S-dermatodea_contig10097:4801..8274+ (Saccorhiza dermatodea SderLu1190fm monoicous)
AGCCAGCTGAACAGCTGAGAGCTGGAGCAGCTGAGGCGAACTGAGAGCAC
GCCAGGGCGACCGAGGAAGCGTCTGAGTTCGGCGCGCGAGTGATCTGGGC
TTAGGGATGATATCTTGCTGTGCAAAATCTGGGGTTTTCAGAGGTAAGCA
AAGTGAGCATAGCTATTACACTGCAGTGGTAGAGGCATTATTGAACTACA
ATAATAGCAGCACGGGAGATTCATATTTAGTACTACTTCATTACAGCTGG
ACATCGGGGTCGTTGTCGGGTGTATCTGACGCCGAGTCTACGTACATACA
TATCGTACAATAGAGCTACAGATGCAGCAGTAGCAGTCTCTCAAGGACCA
CATGAATGATATTACTTGGGCTAAATAAGGTATTAGGTTCCTTCTTAGAT
CATTCTTCGTTTGATATCCCTGCAGGGGACAGGGGACTCGTACTTGTGCA
TCCACCCGTGATCACAATCTCCGATGTTTGAGCAGCATTGTATTTTGTAC
ACAGGTCATTACGTCAGTACACCCTCATAGATACAAAGGTGAGGTGGACC
TGTGTCTGTGGTCAGCCTCGGTCTGAATTACCTTTCTCATTTCGATCATA
TGATACATACAGCAGTAGCTGGTGCTGTCGTATCGTGTTACAATAAGAAT
ACCGGCAGGTTAGCTTCTTAGTTTTGTTATCCCGACACCGAGCTCGATGC
ATACGCTGCTGTACCGCACACCATAAAAAAATATGGTTTGCGGTTCACCT
CACTCTCACCGTCCGTGGGGCTGCGCTGGGACTAAGAATTCACACATTAC
AGGGCATCTCATACATGTACGAATATCGGACATTTCCAGGAACATGGCAG
GCGGTGCAGAATCACAAAGCGAGGAAGCTCGCCGACAGTAGCTTTCCAAA
AAGACGACTGACTCTGCACATAAGGTATCGAGTAGCAGTGAGCGTTCGCG
TTCCACCACGCTGCCAAAAAGAGACTCAAAGCTCAACATTTTGCGGTTCT
CCGCGCCCTCTGCTCTGTTCCTATCACGCCGTCAGGGGCGACGCAACGAT
GGTTGCTCCCCTCGTGCACAGACTTGTTGCGGAAGGGGCTTGCGCCATGC
TCGTCCGTACCATCTTCGTCAAGATACCGTCTCTCGACGGTAGCGACGCC
GCTGGCTCGTCGCCGGCCATATTCTCGACCGTCCGATGCGATCATCAGTC
AGACCACCCTCTCCCGAAGAAGAGCCTTCTCGTCCTACGACTGGAAGAAC
ATGAGCAAGTACGCACAGGAGGCAAACAAGGAATTTTCTAAGCTCACCCC
GGAAGAGGTTCGCAAGGCGGGGGAGGATGAGGTGGACAAGGTAGAAAAGG
CGCTCAAAGCAATGGTGCCGTTCAACGACGTGTTTACGGTCCGACGGGAA
GGGCTCGAGGTTTTCGCGGAGCTCGGGCCTCAAGTGGGAACCTACTGGAT
AAAGTTCGACCTTAGCATACAGCAGATCTGCATGATGTCGCCGAGGTCGG
GGCCTCATAGATACATTTACGACAAGGGCTCGGGGTGAGTGTGCGCTGCT
ATAATAGGCGTTTCCGTTCTTGTCTCTTGTGTCAGGCACAAACACCCCAC
AAGACTGAGGGAGCATTGCGAGTTGAGGGCTCTATGATAACTAATATGTG
TTCCTCGACATTCTAACATACCACGTACATGTTCGCAAGGGAGACGCACC
ACATACTCGAAGAGGAAGGCCTGCTTTTCTCAGCGAAGTGATGCTGATGA
GCTAGACGCCGCTGTCCGCTGCAACGTTACTTTTTTATCGTCTCCTGCCT
CACTCGATAATAATTTTCCCGACATTTTCCTGCCGTCCCGCTCTCCGAAT
GTGGGTCATGCAGGCGGTGGATTGGAGAGGCGGACAAGCACGACTTTCAA
GGGATGCTCACGCGAGACATTATTCAGTCAGCCTGCGGCATCCCCGAGTT
CTAGGCTCCTAAGGCTTGTGGGGGGTGCGCATCGAGAATATGACTGTACT
GTGAGGGCGAGGGTGGGGTCGGGAGATGTAGATTCTGGCGCCATACATCC
GTGGATTGGCCCGTGTTTTCGCTTTGTGCTCCCTACTCGTAGTGGTCTTG
GATTTGCTTCTCCTAGTTGAAATTGTATATTATCCTTGTTGAAAGCCCAC
AGGGTGTCTTGGAGAAGCACGCCGGGTATTGGGACACGTTCGGTCGATAT
TTTGCGAGGGGGGCCTCAAAGCCGGCAGAGTCAAGCCAGGGCCGCGTGAG
CCGTGCTTCATGGGCCAGTGCCACAGGGGAAGGGGCTGCTGCGCGTGTAG
ATTCCATCACTTGCGGCGTGTATCGATTGAGTAACGTGCGCGACGCTGCT
GTCTGTTCGGTACGTACCGCCAATCTTCTGCTTTGCCAATCTTTAGCTTT
TCGGCAAAAGATCCGTGGTGTAAGTTTTGGCATTGGAAACTTTGAATTCC
GCATATATCTATAGAACATTAGCCAAGCAATGCCAGTATAGCTGGCGCTC
GCTCACAGGCGTGTACAGGAATATTGGCAGACCGATGCTCTATACAAGTA
TACTTTAAGGTTGAAAATGTTACTGAGGTCAAGCCAGATGACGTGGAATT
TGTCATAATATCCGTTTCACTATACCTGCTTCCGATCGGAGCTCCATGGC
ATGCGTCGAAGTAAGGGCAGCTGCTATTTAGGCGAGGAGGGGGCAGCCCC
AACATCGGGACGCGGGCGTGTGCGGAGCAACAGAGCCGTGGAGAGTTTAC
GGGTTCAGAGAGGATAGCCTCTATTGTCTACTGGTACCATTCAATAACAA
CCGTTATTTTGGCCGTATGCTACAACGTTTGGCTCTCTGATTTCGTACGA
TACACGCATGCTTACCATCCATTGTATGCATGCACTGTTCCATCCCAGTT
GAGCTCCCAAGAACCGAATGGATTCTCCAAACAAAACCTTGTCACTTGTC
ACGCGCTTCCGGTGTAGCACCTGGTTTTTTACCGTGACTTGTGCGTGACG
GGTCGAACCGCAAAATGTAGCGACCACGCTGCTCCACACGTTTGTATTTG
CCGATATACATCACTAAAGCTGTCTGCCAAACGAAGTACAGCATACCCAC
GAGCTCGCACGTAGTTTGCCCTGCGGCCACCAACACGACCCCAGTACAAG
ACCTCACGCAGCAGGTCTGTTGCAGTCTTACAGCCTTACCGTTTCACATA
ATGATACTGGTACAACAATAATGTAGAGGGGTCGCTGGTGTACTATACGC
AGCCCTGTAACGCTACGCCCACGTATGCTACGCCAACGGAATAGCCACAG
CACACCGCACACCAACGGATGGAAGGTTCTCGCCAGTCTCTCGGATACCA
TAGCGAATCCTGAACAAGAAGATTTTTCGAATACAAGGCAGCGATGCAAC
TGACAGCAGTAATCCTACTGCTGCTGTACAGCGAGTGACAGCAGTCGCTC
AAGCTGTAGCTGGCTTTCGTGGCT
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Coding sequence (CDS) from alignment at S-dermatodea_contig10097:4801..8274+

>mRNA_S-dermatodea_contig10097.64.1 ID=mRNA_S-dermatodea_contig10097.64.1|Name=mRNA_S-dermatodea_contig10097.64.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=CDS|length=1254bp|location=Sequence derived from alignment at S-dermatodea_contig10097:4801..8274+ (Saccorhiza dermatodea SderLu1190fm monoicous)
ATGATATCTTGCTGTGCAAAATCTGGGGTTTTCAGAGATGATATCTTGCT
GTGCAAAATCTGGGGTTTTCAGAGGGGCGACGCAACGATGGTTGCTCCCC
TCGTGCACAGACTTGTTGCGGAAGGGGCTTGCGCCATGCTCGTCCGTACC
ATCTTCGTCAAGATACCGTCTCTCGACGGTAGCGACGCCGCTGGCTCGTC
GCCGGCCATATTCTCGACCGTCCGATGCGATCATCAGTCAGACCACCCTC
TCCCGAAGAAGAGCCTTCTCGTCCTACGACTGGAAGAACATGAGCAAGTA
CGCACAGGAGGCAAACAAGGAATTTTCTAAGCTCACCCCGGAAGAGGTTC
GCAAGGCGGGGGAGGATGAGGTGGACAAGGTAGAAAAGGCGCTCAAAGCA
ATGGTGCCGTTCAACGACGTGTTTACGGTCCGACGGGAAGGGCTCGAGGT
TTTCGCGGAGCTCGGGCCTCAAGTGGGAACCTACTGGATAAAGTTCGACC
TTAGCATACAGCAGATCTGCATGATGTCGCCGAGGTCGGGGCCTCATAGA
TACATTTACGACAAGGGCTCGGGGGGCGACGCAACGATGGTTGCTCCCCT
CGTGCACAGACTTGTTGCGGAAGGGGCTTGCGCCATGCTCGTCCGTACCA
TCTTCGTCAAGATACCGTCTCTCGACGGTAGCGACGCCGCTGGCTCGTCG
CCGGCCATATTCTCGACCGTCCGATGCGATCATCAGTCAGACCACCCTCT
CCCGAAGAAGAGCCTTCTCGTCCTACGACTGGAAGAACATGAGCAAGTAC
GCACAGGAGGCAAACAAGGAATTTTCTAAGCTCACCCCGGAAGAGGTTCG
CAAGGCGGGGGAGGATGAGGTGGACAAGGTAGAAAAGGCGCTCAAAGCAA
TGGTGCCGTTCAACGACGTGTTTACGGTCCGACGGGAAGGGCTCGAGGTT
TTCGCGGAGCTCGGGCCTCAAGTGGGAACCTACTGGATAAAGTTCGACCT
TAGCATACAGCAGATCTGCATGATGTCGCCGAGGTCGGGGCCTCATAGAT
ACATTTACGACAAGGGCTCGGGGCGGTGGATTGGAGAGGCGGACAAGCAC
GACTTTCAAGGGATGCTCACGCGAGACATTATTCAGTCAGCCTGCGGCAT
CCCCGAGTTCTAGGCGGTGGATTGGAGAGGCGGACAAGCACGACTTTCAA
GGGATGCTCACGCGAGACATTATTCAGTCAGCCTGCGGCATCCCCGAGTT
CTAG
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