mRNA_P-littoralis_Contig723.6.4 (mRNA) Pylaiella littoralis U1_48

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1284 bits (3323), Expect = 0.000e+0
Identity = 676/1035 (65.31%), Postives = 794/1035 (76.71%), Query Frame = 2

Query:   44 TPSLAVDLHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGSDTA------------DHPAKLRSTYGDGMVLGNVRGLSVRLSPGTLPAAS-QSLAVNFIRDWHAQLMSESLDLETVSFWSDPDADRSVQDKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLPSEDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVLGDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSD-DMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCYGDELPGCAGGCIMTSEVDEMLNNEYFDIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLC-NGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSMESGAWKVGVSAKGMLTDEQSYSLVITGAIRTS 3103
            +P L VDL KIQ I  SSA  DFA +  AY+AAS+WY+ RL  DA+ E    PY+ CADY +GR A  SL+  F  SA+HRVSN+EADG+CFIVTA  ++ T  + AP +F L+SA P LPS+KLATSLLDHG DT+            DH A LRSTYG+GM L NV GLSVRLSPG L A   +S A  F+RDWHA L SES+++   SFWSD DADRS  D  RVREWSRAAAVV+GLASK  R VGEIC   +L++RH+ DDL+ VEG++HLLP +  PSE + AC+MGLLSQLAAKPEVLRVSPL  A+  NAVA+A+ +  TTTE PLLDAGL+G GEVIQVVD+GLDE+SCFFADDDGLQ+EHG L++GVR E DGNVS VLG+YSF +DMSRRK+VQYI ++K  D+  +      SS  DSFTY SDYLGDPYDYD  D+ Y SY D    YYS+ WSE+SFCA VLDDLSY+ S+ FDDTSYSS             +S SFTGGF+KDTVGGHGTWTAG AAGAIS +S + +E CYGDELPGCAGGCI  S+VD ML++  FD+D+FCPMY CDG+ D+  S CLSDDP E L+Q+ G+APGAQI++FD SY G + Y  +AGN +W SA+ETGAKIH+NSWG AT C+LTE EYLYDT+MYENPE LLIF+AGN+GG++D+P R  CTV SP LGKN L VGATSSG    + TG DG LVYE++G+TDYSPEGYPWIC  PFLG PS+STE A ++T A+FSS GPT D RIKP+VVAPGD QV SASSDGT+ +SC+LV   GTSASCP+VAGAAALVRQYFKD SF+AKD  +RG C   SSS QCS FAPSAATVKAM+INSA +MGG +EP+  RGFGRVHLEAGMPMDG G  GL V +++    + DGE+ E V +DGDAG+ELRATL W+DPPTTALSA QLQHDLDL V APSGA YTMWESGV DTVNVVERV+VP  SMESG W V VSAK +LTDEQ YSLV+TGAI +S
Sbjct:    7 SPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAA-EGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGST------SSGQDSFTYVSDYLGDPYDYD--DSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSS-----------TSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGD-QVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAA----IADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSS 1016          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 1172 bits (3031), Expect = 0.000e+0
Identity = 643/1047 (61.41%), Postives = 748/1047 (71.44%), Query Frame = 2

Query:   23 AGFLLLATPS--LAVDLHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGSDTADH------------PAKLRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDPDADRSVQDKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLPSEDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVLGDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDY----LGDPYDYDYDDTYYTSYSD-DMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCYGDELPGCAGGCIMTSEVDEMLNNEYFDIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLC-NGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSMESGAWKVGVSAKGMLTDEQSYSLVITGAIRTS 3103
            A  LL+A+PS  + VDL KIQ I  SSA  DFA +  AY+AAS W++KRL    + E  + PYI CADY +GR A  SL+  F  SA+HRVSN+E DGSCFIVTA  ++ T  +SAP +F L+SA PFLPS+KLAT LLDHG DT+ H             A LRSTYG+                                                        DRS  D  RVREWSRAAAVV+ LASK  +  GEIC   +L +RH+ DDLL VEG++HLLP + APSEA+ AC+MGLLSQLAAKPEVLRVSPL  A   NAVA+A+ +  TTTE PLLDAGL+G GEVIQVVDTGLDE+SCFFADDDGLQ+EHG L+ GVR E DGNVS VLG+YSF +DMSRRK+VQYI M+K  D+  +      SS  +SFTYASDY    LGD +D D  D+ Y SY D    Y S+ WSEESFCA+VLDDLSY+ S  FDDTSYS              SS+S TGGF KD VGGHGTWTAG AAGAIS +S + +E CYGDELPGCAGGCI  S+VD ML+N  FD+D+FCPMY CDG+ D+  S CLSDDP E L+Q+ G+APGAQI+M DASYTG D++  +AGN +W SA+ETGAKIHSNSWG+   C+LTE EYLYD++MYENPE LLIF+AGN GG +D+P    CT+ SP LGKNIL VGATSSG SR + TG DG LVYE+ G+TDYSPEGYPWIC  P LG PS+S E A ++T A+FSS GPT D RIKP+VVAPGD QV SASSDGT+ +SC L+   GTSASCP+VAGAAALVRQYFKD SF+AKD ++RG C   SSS QCS FAPSAATVKAM+INSA +MGG +EP+  RGFGRVHLEAGMPMDG G  GLLV +   A+I  D E+ E V +DGDAG ELRATL W+DPPTTALSA QLQHDLDL V APSGA YTMWESG  DTVNVVERV+VP  SMESG W V VSAKG+LTDEQSYSLV+TGAI +S
Sbjct:    9 AAILLVASPSAVIGVDLGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAH--APEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGE--------------------------------------------------------DRSQYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGST------SSGQNSFTYASDYIRDYLGDAFDDD--DSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSAS---------SSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGD-QVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVE---ATIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGAIYSS 976          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 709 bits (1829), Expect = 1.100e-234
Identity = 397/773 (51.36%), Postives = 492/773 (63.65%), Query Frame = 2

Query:  323 VTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGSDTA------------DHPAKLRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDPDADRSVQDKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLPSEDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTER---PLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVLGDYSFAYDMSRRKV-------------------------------------------------VQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSD-DMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCYGDELPGCAGGCIMTSEVDEMLNNEYFDIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVG 2446
            V A  ++ T  + AP +F L+S  P L S+KLATSLLDHG DT+            DH A LRSTYG+G+                                         ++   SFWSD DADRS  D  RVREWSRAAAVV+GLASK  R VGEIC   +L++RH+ DDL+ VEG+ +LLP +  PSE + AC+MGLLSQLAAKPEVL VSPL  A   NAVA+A  +  T  +R   P    G +         D+GLD++SCFFADDDGLQ+EHG+L++GVR E DGNVS  LG+YSF +DMSRRKV                                                 VQ I ++K   +  +      SS  DSFT+ SD++GDP+DYD  D+ Y SY D    YYS+ WSE+SFCA  LDDLSY+LS+ +DDTSY+S           P +S SFTGGF++DT+GGHGT T+G AAGAIS +S + +E CYGDELPGCAGGCI  S+VD ML++  F + +FCPMY CDG+ D+  S CLSDDP E L+Q+ G+APGAQI++FD SY G + Y  +AGN +W SA+ETGAKIH+NSWG AT C+LTE EY+YDT+MYENPE LLIFSAGN+GG+ D+P R  CTV SP LGKNIL VGATSSG    + TG DG L  E++G+TDYSPEGYPWIC  P LG P +STE A ++T  +FSS GPT D +IKP VVAPG++ V SASSDGT+E+SC+L G
Sbjct:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGV-----------------------------------------NMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGST------SSGQDSFTFFSDFIGDPFDYD--DSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYAS-----------PSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAG 713          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 674 bits (1739), Expect = 1.290e-217
Identity = 440/1042 (42.23%), Postives = 577/1042 (55.37%), Query Frame = 2

Query:   65 LHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGS--DTADHPA----KLRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDP-----DADRSVQDKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLPS--------EDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVL------GDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEG--CYGDELPGCAGGCIMTSEVDEMLNNEYF-----DIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSMESGAWKVGVSAKGMLTDEQSYSLVITGAI 3094
            L  ++  L ++A  DF G+  A+ AA+ W  +RL R  SQ+  + P++ CA+YG GR  F+ LK      A+  VS+SE  G+CF+ TAS A  +  +     FGL S  PF  ++KLA  L+DH    +    P+    +L + +G  M + NV GL+V+L+P TLPA S   A +FI D    LMS S+DL + +FWSDP     +   S    +R R+WS+AA+VV  L+       G+IC+   + +   GDD L V G+DHLL S        E+  +E  +ACFMGL+S  A +PEVLRVSP +  + LN  A  I +  + T+ PL DAGL+G GEVIQVVDTGLDE+SCFF DD G +++HG+ +E +   + G+  ++       GD  F Y   RRKV+QYI +                  PDS     DY+            +++    +Y +  P                      D+                          F +D   GHGT TAG AAGA       P E   C GDE  GC GGCI  SE  + L +        D+D  CPMY CDG  D     CLSD+  ETL +N GMA GA++++FD  +  F   +E  GN VWE  +E G K+HS S G  T C   E E LYD +MYENPE LLIF+AGN+G  +    R+ CT+ SP +GKN+L VGA++SG +R   T                  EG                   A I+  A FSSYG T+D RIKPEVVAPGD  V+SA+SDGT+ +SC L    GTS SCP+VAGAAA+VRQYF D++FY  D +ARG C  S  F C GF+PSAATVKA+LINSA++MG  ++PD YRGFGR+HLE GMP++G G L L VAD+   SI  D       ++D DAG+ELRATL WID P T+ SA QL +DLDLVV +PSG  Y MW S V D  NV ERV+V A  +E+G W V V +  ++TD QSYSLV+ GAI
Sbjct:   34 LELVENGLATTAKDDFNGHARAFEAAATWNGERLRR--SQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAG-AESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGD--FTYTEERRKVIQYIELVN----------------PDS-----DYVA-----------FSTTQGGVYPFLPP----------------------DE--------------------------FGQDDSAGHGTHTAGSAAGATLND---PAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTDP----CLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFG-LIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSGDTRVIST------------------EG------------------GAGIDMVADFSSYGFTTDGRIKPEVVAPGD-MVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFC--SDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAI 939          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 643 bits (1658), Expect = 4.790e-206
Identity = 424/1041 (40.73%), Postives = 559/1041 (53.70%), Query Frame = 2

Query:   65 LHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDH--GSDTADHPAK------LRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDPDAD------RSVQDKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLPS--------EDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVLGDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEG--CYGDELPGCAGGCI-MTSEVDEMLN----NEYFDIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEV--NIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSMESGAWKVGVSAKGMLTDEQSYSLVITGAI 3094
            L  ++  L ++A  DF G+  A+ AA+ W  +RL    SQE+ + P++ CA YG+G  A + LK      A+  VS+SE  G+CF  +AS A     +     FGL +  PF   +KLA  L+DH    D A+  A+      L + +G  M   NV GLSV L+PGTL AA  S A +FI      LMS S+DL + +FWSDP  D       S    +R R+W +AA+VV  L+       G+IC+  R+ + H GDD L V G+DHLL S        E+  +E  +ACFMGL+S LA +PEVLRVSP    + LNA A  + +  + T+ PL DAGL+G GE+IQV+D+GLDE+SC+F DD G +++HG+ ++                     ++ R  V                                              Y +SYS          S+E+       D SYY                                   +    GHGT TAG AAGA       P E   C   +   C G CI +    D++++     +  D+D  CPMY CD   D     CLSDD  ETL  N GMA GA++A+FD  Y G+DA     GN +WE   E G K+HSNS G    C +  ++ L D +MYEN E LLIF+AGNEGG +    R+TCT+ +PG+GKN L +GA++SG +R   T          +G T                           I+T A FSS+G T+D+RIKPEVVAPGD  V+SA+SDGT+ +SC L    GTS SCP+VAGAAA++RQYF D++FY  D +ARG C  S  F C GF+PSAATVKA+LINSA++MGG +EPD +RGFGRVHLEAGMP++G G L L VAD+   SI   G  +EE   ++D DAG++LRATL WIDPP TA SA QL H+LDLVV +P G TY MW S   D  NV ERV+V A+ + +G W V V A  ++TD QSYSLV+ GAI
Sbjct:   29 LELVENALLTTAKDDFKGHVGAFEAAATWNGERLRH--SQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTL-AARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFD---------------------ELGRAAV----------------------------------------------YSSSYS----------SDETATIFNGGDFSYYP----------------------------------ERRKAGHGTHTAGSAAGATLND---PAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTT---CLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGDTRVIST----------VGATG--------------------------IDTVAEFSSWGFTTDDRIKPEVVAPGDS-VYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFC--SDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIA--GGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAI 903          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 616 bits (1588), Expect = 1.590e-194
Identity = 420/1081 (38.85%), Postives = 564/1081 (52.17%), Query Frame = 2

Query:   65 LHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGS------DTADHPAKLRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDPDADRSVQ-----DKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLPS-------EDAPSEAKM--ACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFA-DDDGLQIEHGHLYEG---------------------------------------VRFEADGNVSMVLGDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDE--GCYGDELPGCAGGCIMTSE---VDEML--NNEYFDIDLFCPM---YECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGT-EENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSMESGAWKVGVSAKGMLTDEQSYSLVITGAI 3094
            L  ++  L ++A  DF G+  A+ AA+ W  +RL     Q++ + P++ CA+YG GR  F+ LK      A+  VS+SE  G+CF  +AS A  +  +     FGL S  PF  ++KLA  L+DH        +T     +L + +G  M + NV GLS+ L+PGTL + S   A  FI D    LM  S+DL + +FWSD +     Q       +R R+WS+AA+VV  L+       G+IC+  R+ + H   D+L V G+DHLL S        D    A++  ACFMGL+S LA+KPEVLRV+P      LNA A A  +  T T+ PL DAGL+G G+V+QV+D+G+DESSCFFA D+ G  + HGH +E                                        +  E DG      GD++   D  RRK++QYIN+ K         D    S  DSFT +   LG  Y                     PW                                                 F  D   GHGT  AG  AGA       P E   C   E   C G C+   E   VD++   N E  D+D  CP+   Y+CD    +    CLSDD AETL  N GMA GA++A+FDA Y  F     + GN +WE   E   KIHSNS+G    C L   + LYD +M  NPE LLIF+AGN+G  +    R+TCT+ SP + KN+L VGA++SG +R S T                           P LG          I+T A FSSYGPTSD+RIKPEV+APGD  ++SA+SDG+ + +SC L   +GTS S P+VAGA+A++RQYF +++F+A D +ARG C+  +   C  F+PS+AT+KA++INSA++MGG +EPD  RGFGR+HLEAGMP+ G G+L L V+DS+  SI          +++  A ++LR TL W+DPP T  SA QL HDLDLVV +P+G  YTMW SGV D+ NV ERV+V A  +E+G W V + A  + TD QSYSLV+ GAI
Sbjct:   24 LELVENALSTTAKDDFNGHARAFEAAATWNGERLRH--RQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVE-AEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDG------GDFTIFPD--RRKIIQYINLIKS--------DDTEGSHGDSFTTS---LGQQY---------------------PWLPAD--------------------------------------------RFEYDYEAGHGTHVAGSVAGATLN---TPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDV---LETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFG-LSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERR----RSTCTMSSPAIAKNVLAVGASTSGDTRLSAT---------------------------PGLG----------IDTVAVFSSYGPTSDSRIKPEVLAPGDA-IYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG--CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 966          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 609 bits (1571), Expect = 1.700e-194
Identity = 402/1020 (39.41%), Postives = 537/1020 (52.65%), Query Frame = 2

Query:   47 PSLAVDLHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHG---SDTADHPAKLRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDPDADRSVQ-----DKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLP-------SEDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVLGDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCYGDELPGCAGGCI---MTSEVDEM--LNNEY----FDIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEE---VNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWE-SGVADTVNVVERVVVPASSMESGAW 3022
            PSL+V    ++  L ++A   F G+  A+  A+ W  + +LR   +++ + P++ C +Y  G +    L+      A+  V++S   G+CF VTAS A       +P  +GL S  PF  ++K+A  +L+HG      +  P +L +T+G  M  GNV GL++ LSPGTLPA S    + FI +    LMS+S+DL   + WSDP A            +R REWS AA +V  LA       G+IC+   + M + G+DLL V G+DHLL        S++  +E ++ACFMGL+S L+ + EVLRVSP +  + LNAVA  I +  T TE PL DAGL+G G+VIQV+DTGLDE+SCFF DDDG ++ HGH YE +                                                                                               E++                             P SS +      +D V GHGT  AG A GA           C G E+PGC GGCI    TS  D++  L  EY     DID  CPM +CD   D     CLSDD  +TL  + GMA GA++A FD    G      + GN +WE  +E G KIHS S G    C + E + LYD +MY NPE L++ +AGN G  +D  GRT CT+ SP +GKN L VGATSSG +R + T ADG L+            GY                  AD++T A FSSYGPT D RIKPEVVAPGD  ++SA SDG++ +SC L   SGTS +CP+VAGA+A+VRQYF D+SFY  D +ARG  + S   +C  F+PS+ATVKA+LINSA++MGG +EPD YRGFGR+HLE GMP+DG G+L L VADSS  S+    E+  +    ++D +A ++ RATL WIDPP ++ S  QL HDLDL V +PSGAT+ MW  SG  D+ NV ERV++ A  +ESG +
Sbjct:   32 PSLSV----VEGSLLTTAQDSFDGHAKAFETAAQWTGE-ILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSP-YYGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGM-FIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEAL----------------------------------------------------------------------------------------------GEII-----------------------------PSSSSA------RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPR---CLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSG-THFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLID--------GTNGY------------------ADVDTVAQFSSYGPTQDGRIKPEVVAPGDA-IYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGR-RCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLP---ELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTY 878          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 596 bits (1537), Expect = 6.280e-188
Identity = 418/1085 (38.53%), Postives = 570/1085 (52.53%), Query Frame = 2

Query:    5 SFLAAAAGFLLLATPSLAVDLHKI---QQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGS------------DTADHPA-KLRSTYGDGMVLGNVRGLSVRLSPGTLPAAS--QSLAVNFIRDWHAQLMSESLDLETVSFWSD-----PDADRSVQDKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLL--------PSEDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFE------ADGNVSMVLGDYS---FAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCYGDELPGCAGGCIMTSEVDEMLNNEYFDIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSME----SGAWKVGVSAKGMLT-DEQSYSLVITGAIRTSSQGL*AV 3124
            +F  AA   LL++T S A  +  +   +Q L ++A ADF  +  A+  AS W +        ++    PY+ C ++GEGR A+  L+   G  A+  VS++   G+CF+VTAS A     LS+  +F   + G F  ++K+A  LLDHGS             T+D  A +L +T+G  M + +  GL V LSPGTLP+ S   + A  FI++    L S SLDL  ++ WSD      D   S    +R REWSRAA +V   +        ++C  +++ + H  +D+L V G+DHLL         SE+  +E +MAC+MGL+S LA++ EVL+V+   R   LNA A A  +  +    PL DAGL+G+GEVIQ++D G++E++CF    DG ++EHG+ ++ V         +  + SM   +++   F+YD  RRKV+QYI++       D+GD    SS+   F  A  ++ D                                   DDL                                           GHGT  A   AG+             G E+ GCAGGCI  S       +   DI+  CP + C GD       CL DD A+TL ++ GMA GA+IA  D  Y  +  Y + AGN +WE+  + G KIHSNS+G    C+LT  + LYD YMYEN E LL+FSAGN G   D   R TCT+ SPG+ KN+LTVG+TSSG +R S T  DGR              GY            +   E + I+  + FS YG T D RIKPEVVAPGD  ++SAS +G + +SCEL+  +GTS S  V +GAAA+VRQYF+D SFY+ D  +RG C+    F C  F+P A TVKAMLINSA+VMGGG EPD  RGFGR+HLEAGMP+ G G + L VADSS   +  D ++    N+D DAG+ELRATL W DP  ++LS  QL +DLDL V AP G  Y MW+    DT NV ERV+VPA  +     +  W+V V  +     D  S+SLV+TGAI    +G  AV
Sbjct:    4 TFRGAATSVLLISTVSGASQVPSLGFFEQTLLTTAGADF-DSEAAFAFASAWQA------GDRQGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQ-LSSWEDF--TTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINP----DTGD---GSSSGMPFVSADSFVSD-----------------------------------DDL-------------------------------------------GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGDQ------CLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYS-YGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGR--------------GY------------ADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGD-MIYSASGNGGD-SSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCD--QGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIEGNSAV 953          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 585 bits (1508), Expect = 1.670e-184
Identity = 402/1045 (38.47%), Postives = 542/1045 (51.87%), Query Frame = 2

Query:   47 PSLAVDLHKIQQILESSAPADFAGNPVAYTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAFASLKNTFGTSAIHRVSNSEADGSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHG---SDTADHPAKLRSTYGDGMVLGNVRGLSVRLSPGTLPAASQSLAVNFIRDWHAQLMSESLDLETVSFWSDPDADRSVQ-----DKMRVREWSRAAAVVEGLASKHDRPVGEICNLKRLRMRHIGDDLLSVEGVDHLLP-------SEDAPSEAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQIEHGHLYEGVRFEADGNVSMVLGDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCYGDELPGCAGGCI---MTSEVDEML-----NNEYF-DIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDASYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWE-SGVADTVNVVERVVVPASSMESGAWKVGVSAKGMLTDEQSYSLVITGAIRTSS 3106
            PSL V    ++  L ++A   F G+   +  A+ W    ++R   +++ + P++ C +Y  G    + L+      A+  V++S   G+CF VTAS A        P  +GL S  PF  ++K+A  +L+HG      +  P +L +T+G  M  GNV GL++ LSPGTLPA S    + FI +    LMS+S+DL   + WSDP A            +R REWS AA +V  LA       G+IC+   + + H G+DLL V G+DHLL        S++  +E ++ACFMGL+S L+++ EV RVSP +  + LNAVA  I +  T T+ PL  AGL+G G+VIQV+                                                       QYIN+ K AD           S P S T                   TS  + +++ S                                                   GF  D V  HGT TAG A GA           C G E+PGC GGCI    TS  D++L     N+ Y  DID  CPM  CD + +    +CLSD   +TL  + GMA GA+++ FD  Y G   + ++ GN +WE  +E G KIHS S+G    C + + + LYD +MY NPE L++ +AGN G  +D  GRT CT+ SP +GKN L VGATSSG +R   T ADG       G   YS                       D++T A FSSYGPT D RIKPEVVAPGD  V+SA SDG++ +SC L   SGTS +CP+VAGA+A+VRQYF D+SFY  D +ARG C+ S   QC  F+PS+ATVKA+LINSA++MGG +EPD +RGFGR+HLE GMP+DG G+L L VADSS  S+          ++D +A ++ RATL WIDPP ++ S  QL HDLDL V +PSGAT+ MW  SG  D+ NV ERV++ A ++ESG + V VS   +LTD Q+YSLV+ GA+  SS
Sbjct:   32 PSLGV----VEGSLLTTAHESFDGHAKGFETAAQWTGD-IIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNP-YYGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGM-FIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI-------------------------------------------------------QYINLIK-ADS---------VSGPASST-------------------TSGGNRVWWVSPD-------------------------------------------------GFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENE---PWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIED--GRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRA---DGTNGYS-----------------------DVDTVAVFSSYGPTQDGRIKPEVVAPGDA-VYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGR-QCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAMVYSS 902          

BLAST of mRNA_P-littoralis_Contig723.6.4 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 551 bits (1419), Expect = 3.020e-169
Identity = 420/1125 (37.33%), Postives = 553/1125 (49.16%), Query Frame = 2

Query:  131 YTAASNWYSKRLLRDASQEDLKVPYIGCADYGEGRRAF-ASLKNTFGTSAIH-RVSNSEAD-GSCFIVTASTAAGTPFLSAPGNFGLVSAGPFLPSMKLATSLLDHGSDTADH---PAKLRSTYGD----------------------------------------------GMVLGNVRGLSVRLSPGTLPAASQ---------SLAVNFIRDWHAQLMSESLDLETVSFWS--------DPDADRSVQD-------KMRVREWSRAAAVVEGLASKHDRP-VGEICNLKRLRMRHIGDDLLSVEGVDHLLPS------EDAPS--EAKMACFMGLLSQLAAKPEVLRVSPLYRAKRLNAVASAITEGGTTTERPLLDAGLNGAGEVIQVVDTGLDESSCFFADDDGLQ-IEHGHLYEGVRFEA----------DGNVSMVL----GDYSFAYDMSRRKVVQYINMYKRADENDSGDDXXXSSTPDSFTYASDYLGDPYDYDYDDTYYTSYSDDMYYYSEPWSEESFCAEVLDDLSYYLSFFFDDTSYSSXXXXXXXXXXXPYSSVSFTGGFYKDTVGGHGTWTAGIAAGAISERSGVPDEGCY------GDELPGCAGGCIMTSEV---DEMLNN---EYF-------DIDLFCPMYECDGDADMAYSYCLSDDPAETLYQNSGMAPGAQIAMFDA--SYTGFDAYVEYAGNYVWESAVETGAKIHSNSWGFATFCELTEFEYLYDTYMYENPETLLIFSAGNEGGFKDVPGRTTCTVRSPGLGKNILTVGATSSGRSRASDTGADGRLVYEKIGITDYSPEGYPWICAFPFLGVPSSSTEEADINTGAWFSSYGPTSDNRIKPEVVAPGDKQVFSASSDGTEENSCELVGGSGTSASCPVVAGAAALVRQYFKDQSFYAKDFSARGLCNGSSSFQCSGFAPSAATVKAMLINSADVMGGGTEPDQYRGFGRVHLEAGMPMDGTGALGLLVADSSKASIVPDGEVVEEVNIDGDAGIELRATLCWIDPPTTALSATQLQHDLDLVVTAPSGATYTMWESGVADTVNVVERVVVPASSM---------------ESGAWKVGVSAKGMLTDE-QSYSLVITGAI 3094
            + AA  W+  RL      +    PY+ CA     R    A+L +  G  AI  RV  S A+ G C + TA+             F   +A P   ++KLA  LLDH  D  D      +LR T+G+                                              G     V GL+V L+PG L A ++         + +     +W  +LMS+SLD+   SFWS        D  A     D       ++  REW+RAA V+ GL      P +G++C+   L + H  DDLL+++G+DHLLP        + P   E K+ACFM LLS LA++PEVLRVSP    K LNA A    +  + TE P+ DAGL+G GEVIQ+VDTGLD +SCFF D+DG + + HG+ +E +  EA          D NV +      GD++F  D+SRRK +     +   D           S   S  +A   LGD                        WS E+                 + T+Y               S+  F     +D  GGHGT TAG AAGA+         G +      G EL  C GGCI   +    D+++     +YF       D+D  CP  +CD   D+   YCL DD AETL Q+ G+A GAQ+A+FD    YTG    ++ AGN +WE A+E G+K+ SNSWG   +C+    + L+D +MY N + LL+F+AGN+G   D  G   CT+ +P L KN+L VGA++SG  R S T  DG                                +   DI+  A+FSS+GPT DNRIKPEVVAPGD  ++SA+  G    +C L   +GTS S P+VAGAAALVRQYFK+++FYA D   RG C+   SF C  FAPSAATVKAMLINSA++MGG  EPD  RGFGRVHLEAGMP+ G G   L VADS+  S            +D  AGI+LRATL WIDPP T  S+ QL HDLDL V +P+G  Y+MW  G  D++N  ERV+VPAS++                 G W V V +K +  +E QSYSLV+TGAI
Sbjct:   53 FKAAVEWHEARL-----DDARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFSSWAAFPS--ALKLAPGLLDHPHDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNF--DLSRRKGLVVDAAHPSCD-----------SPNISRRFA---LGDV---------------------RAWSGENPACTAN-----------NSTNYGESWATSAGEQYPWISAAEFA----EDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELS-CVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVG--IDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD--DWSG---CTMGTPALAKNVLAVGASTSGPGRLSFTDEDG--------------------------DPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGD-MIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCD---SFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAI 1077