mRNA_P-littoralis_Contig861.2.1 (mRNA) Pylaiella littoralis U1_48

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 844 bits (2181), Expect = 4.200e-293
Identity = 465/816 (56.99%), Postives = 567/816 (69.49%), Query Frame = 1

Query:    1 LQAVENVLLTTAQDDFDGGVEAFEAAARWSAERLLEDGDG-RAPHLACTEYENSREVFERLNAFLSPRAVRPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDAEERMGRSEDMNAAAHGPDRLRARHGASMCRDTAEGLSVELTPGTLPAHAPGADGFMSALHRGLMSESLDLHSSNFWSHTTNKGGEHLATPAGALRGRDWSKAATVVHELSEAAGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDN------RATNKIFT---------FDDASVD---GRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAETIACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHAASHAFFDIDRLC---SDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            L+ VEN L TTA+DDF+G   AFEAAA W+ ERL    D  RAPHL C EY + RE F RL  FLSP AVR  SHS+  GACFFA+ASH +A+ I E +E QF L  SF+ FPSALKLAPGL+ H +  D D E              G DRL ARHGA M     EGLS+ELTPGTL + +  A+ F+S     LM  S+DLHS+NFWS     GGE L+ P GA+R RDWSKAA+VVHELSEAAGTSPG ICSW+ V+++H   DVLL++GLDHLL++G GAGG+   GE+AELH+ACFMGLVSF AS+ EV+RVAPR    L NA+ARANIQSAT+T+TPLTDAGL+G GQV+QV+DSG+DESSCFFAHD +GD V HG+YF++ G N  +  S  +S T  D+      RAT+   T         FD         R +I+QYINLIK D+T      SFTT  G+++PWL A  FE D  AGHGTHVAGS AGATL TPAE I+C++ + + CVG C++ +  A D+  DDL T +         D+DRLC   SD+ C     + CL ++V+ETLT +GG+A GAKLAIFDA + +     F GNGLWE C EA C++HSNSYG D EC L P+D+LYD +M  NPE+LLIFAAGNDG + R  CT++SPAIAKNVLAVGASTSG TR+SAT          GID +A FS+YG T D RIKPEV+APGD++YSAASDG  D +SCRL+A+AGTSMS PIVAGA+AMIRQYFM+  F+  D+ ARGFC +W GCE F PS+ATLKA + 
Sbjct:   24 LELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQE-QFGLE-SFSPFPSALKLAPGLVDHSQRHDKDKE-----------TTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDD-EANDV--DDLATPNVEIV-----DLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGL-------GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMI 811          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 801 bits (2070), Expect = 2.020e-277
Identity = 454/804 (56.47%), Postives = 551/804 (68.53%), Query Frame = 1

Query:    1 LQAVENVLLTTAQDDFDGGVEAFEAAARWSAERLLEDGDG-RAPHLACTEYENSREVFERLNAFLSPRAVRPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDAEERMGRSEDMNAAAHGPDRLRARHGASMCRDTAEGLSVELTPGTLPAHAPGADGFMSALHRGLMSESLDLHSSNFWSHTTNKGGEHLATPAGALRGRDWSKAATVVHELSEAAGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFN---------YDIFESWTTSATFGDNRATNKIFTFDDASVDGRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAETIACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHAASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            L+ VEN L TTA+DDF+G   AFEAAA W+ ERL    DG RAPHL C EY + RE F RL  FLSP AVR  SHS+  GACF ATASH +A+ I E +E QF L  SF+ FPSALKLAPGL+ H E  + + E            + G DRL ARHG +M     EGL+V+LTP TLPA + GA+ F+  L   LMS S+DLHS+NFWS     GGEHL++  GA+R RDWSKAA+VVHELSEAAGTSPG ICSWD VA+   G+D LL+ GLDHLL++G GAGG  H+ E+ ELHVACFMGLVSFFA R EV+RV+PRH   + N AAR  IQSA+ T+TPLTDAGL+G G+VIQVVD+GLDE+SCFF  D  G++V HGY+F++L            Y +FE+       GD       FT+ +     R +++QYI L+ PD+       +F+T  G  +P+L   EF  D +AGHGTH AGSAAGATL  PAET+ C   + +GCVGGCI+++      T DDLV+   +A   A  D+DRLC  +GC + S   CL + V ETLTK+GG+A GAKL+IFD  F+        GNG+WE C+EAGC++HS S G D  C    S+ILYD+FMYENPE+LLIFAAGNDG   R  CT+ SPAI KNVLAVGASTSG TR+ +T         AGID +A FS+YGFT DGRIKPEVVAPGD VYSAASDG  D +SCRL+A+ GTSMSCPIVAGAAAM+RQYF+D  FY AD+ ARGFC D   CEGF PSAAT+KA + 
Sbjct:   34 LELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQE-QFGLE-SFSPFPSALKLAPGLVDHSESREVEGE-----------PSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGS-HEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDG-GEEVDHGYFFEELFVGSTGSSQLIYYPVFEA-------GD-------FTYTEE----RRKVIQYIELVNPDSDY----VAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESE-----TTDDLVS---YAIQIALPDLDRLCPMYGC-DGSTDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTEGG------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGT-DSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLI 784          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 744 bits (1921), Expect = 2.690e-255
Identity = 432/798 (54.14%), Postives = 521/798 (65.29%), Query Frame = 1

Query:    1 LQAVENVLLTTAQDDFDGGVEAFEAAARWSAERLLEDGDG-RAPHLACTEYENSREVFERLNAFLSPRAVRPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDAEERMGRSEDMNAAAHGPDRLRARHGASMCRDTAEGLSVELTPGTLPAHAPGADGFMSALHRGLMSESLDLHSSNFWSHTTNKG-GEHLATPAGALRGRDWSKAATVVHELSEAAGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASVDGRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAETIACENGKIIGCVGGCINQNASAGDLTD--DDLVTTSEHAASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            L+ VEN LLTTA+DDF G V AFEAAA W+ ERL    +  RAPHLAC  Y +  E   RL  FLSP AVR  SHS+  GACFFA+ASH +AA I E +E QF L  +F+ FPS LKLAPGL+ H E  D+  EE         A + G DRL ARHGA M +   EGLSVELTPGTL A +  A  F++ +   LMS S+DLHS+NFWS     G GEHL++PAGA+R RDW KAA+VVHELSEAA T PG ICSW+ + ++H G+D LL++GLDHLL++G GAGG  H+ E+ ELHVACFMGLVSF A R EV+RV+PR    + NAAAR  IQSA+ T+TPLTDAGL+G G++IQV+DSGLDE+SC+F  D+ G++V HGYYFD+LG                                    R   Y +    D T        T  +G  F +     +   + AGHGTH AGSAAGATL  PAETI C     + CVG CI       DL D  DDLV+ S         D+DRLC  +GC   ++  CL ++V ETL+ +GG+A GAKLAIFD  +    A    GN LWE C EAGC+LHSNS G+D EC +   DIL D+FMYEN E+LLIFAAGN+G   R  CT+N+P I KN LA+GASTSG TR+ +T  A       GID +A FS++GFT D RIKPEVVAPGDSVYSAASDG  D +SCRL+A+ GTSMSCPIVAGAAAMIRQYF+D  FY AD+ ARGFC D   CEGF PSAAT+KA + 
Sbjct:   29 LELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQE-QFGLE-NFSPFPSPLKLAPGLVDHTESHDEAEEE---------ARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGS-HEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDS-GEEVDHGYYFDELG------------------------------------RAAVYSSSYSSDETA-------TIFNGGDFSY-----YPERRKAGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACI-------DLLDATDDLVSISSQTDGT---DLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGAT------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGT-DTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLI 748          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 671 bits (1732), Expect = 4.460e-228
Identity = 390/804 (48.51%), Postives = 497/804 (61.82%), Query Frame = 1

Query:    1 LQAVENVLLTTAQDDFDGGVEAFEAAARWSAE--RLLEDGDGRAPHLACTEYENSREVFERLNAFLSPRAVRPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDAEERMGRSEDMNAAAHGPDRLRARHGASMCRDTAEGLSVELTPGTLPAHAPGADGFMSALHRGLMSESLDLHSSNFWSHTTNKGGEHLATPAGALRGRDWSKAATVVHELSEAAGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASVDGRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAET-IACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHAASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKT--RQDCTVNSPAIAKNVLAVGASTSGSTRISATSA----AEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDG-CEGFVPSAATLKAKIF 2382
            L  VE  LLTTAQD FDG  +AFE AA+W+ E  R  E+ + RAPHL CTEY    +   RL   LSP AVRP +HS   GACFF TASH EA  + +     +  + S + FPSALK+APG+L+HG                ++ A+  P RL   HG  M +   EGL++EL+PGTLPAH+P A  F++ L   LMS+S+DLH  N WS      GEHL TPAGA+R R+WS AAT+VH+L+ A  TSPG ICSWDS+++++ GND+LL++GLDHLL+   GA   G D EA EL VACFMGLVSF ++R+EV+RV+PRH   L NA AR  IQSAT+TE PLTDAGL+G GQVIQV+D+GLDE+SCFF  D  G++V HG+Y++ LG                                                   E + +S + R               D   GHGTH AGSA GAT+ TPAET   C   ++ GCVGGCI+ + ++     DDL T     + +   DIDRLC    C + ++  CL ++V +TL   GG+A GAKLA FD          F GNGLWE C+EAGC++HS S G+D EC +   D+LYD+FMY NPE+L++ AAGN G+    R  CT+ SPAI KN LAVGA++SG TRI+ TSA     + +   A +D +A FS+YG T+DGRIKPEVVAPGD++YSA SDG  D +SCRL A +GTSM+CPIVAGA+AM+RQYFMD  FY AD  ARG+  D    CE F PS+AT+KA + 
Sbjct:   34 LSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDS---PYYGLTSISPFPSALKVAPGVLEHGVS--------------LDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGA--IGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDD-DGEEVPHGHYYEALG---------------------------------------------------EIIPSSSSAR---------------DDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTS---WGDDLFTLMPEYSPYDI-DIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGS-DNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLI 746          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 655 bits (1690), Expect = 6.300e-221
Identity = 385/804 (47.89%), Postives = 485/804 (60.32%), Query Frame = 1

Query:    1 LQAVENVLLTTAQDDFDGGVEAFEAAARWSAE--RLLEDGDGRAPHLACTEYENSREVFERLNAFLSPRAVRPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDAEERMGRSEDMNAAAHGPDRLRARHGASMCRDTAEGLSVELTPGTLPAHAPGADGFMSALHRGLMSESLDLHSSNFWSHTTNKGGEHLATPAGALRGRDWSKAATVVHELSEAAGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASVDGRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAET-IACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHAASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGN--KTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSA----AEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDG-CEGFVPSAATLKAKIF 2382
            L  VE  LLTTA + FDG  + FE AA+W+ +  R  E+ + RAPHL CTEY    E   RL   LSP AVR  +HS   GACFF TASH EA  + +     +  + S + FPSALK+APG+L+HG   D               A+  P RL   HG  M +   EGL++EL+PGTLPAH+P A  F++ L   LMS+S+DLH  N WS      GEHL TPAGA+R R+WS AAT+VH+L+ A  TSPG ICSWDS++I+H GND+LL++GLDHLL+   GA   G D EA EL VACFMGLVSF +SR+EV RV+PRH   L NA AR  IQSAT+T+ PLT AGL+G GQVIQV+                                                                     QYINLIK D+      AS TT  G +  W++   F+ D    HGTH AGSA GAT+ TPAET   C   ++ GCVGGCI+ + ++     DDL+T     +S+   DIDR+C   GC EE+   CL + V +TL   GG+A GAKL+ FD    +     F GNGLWE C+EAGC++HS S+G+D EC +  +D+LYD+FMY NPE+L++ AAGN G     R  CT+ SPAI KN LAVGA++SG TR+  TSA    A+ +   + +D +A FS+YG T+DGRIKPEVVAPGD+VYSA SDG  D +SCRL A +GTSM+CPIVAGA+AM+RQYFMD  FY AD  ARG+C D    CE F PS+AT+KA + 
Sbjct:   34 LGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDN---PYYGLTSISPFPSALKVAPGVLEHGVSRDQ--------------ASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGA--IGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI---------------------------------------------------------------------QYINLIKADSVSGP--ASSTTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTS---WGDDLLTLMPENSSYVA-DIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGS-DVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLI 742          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 641 bits (1653), Expect = 1.530e-214
Identity = 378/806 (46.90%), Postives = 506/806 (62.78%), Query Frame = 1

Query:   13 ENVLLTTAQDDFDGGVEAFEAAARWSAERLLEDGDGRA-PHLACTEYENSREVFERLNAFLSPRAVRPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDA-EERMGRSEDMNAAAHGPDRLRARHGASMCRDTAEGLSVELTPGTLPAHAPG---ADGFMSALHRGLMSESLDLHSSNFWSHTTNKGGEHLATPAGALRGRDWSKAATVVHELSEAAGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASVDGRHRIVQYINLIKPDNTPEELDASFTTRDGRK--FPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAETIACENG-KIIGCVGGCINQNASAGDLTDDDLVTTSEHAASHAFF--DIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFA-GGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQD-CTVNSPAIAKNVLAVGASTSGSTRISATS----AAEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            E  LLTTA  DFD    AF  A+ W A     D  GR+ P+L+C E+   R  + +L  FL  RAV+P S +   GACF  TAS  EAA +       +E   +F  FP+ALK+APGLL HG  D   A  +    + D +A      RL   HGASM  D+A GL VEL+PGTLP+H+ G   A  F+  L + L S SLDLH+   WS    KGG+HL + AGALRGR+WS+AAT+V+E SE+   +P  +C W+ V ++H  NDVLL+AGLDHLLY G    G   + EAAEL +AC+MGLVSF ASR EV++VA  ++    NAAARA IQSA++  TPLTDAGL+G+G+VIQ++D+G++E++CF  H   G +V HGYYFD +  +  +F S + S +      T   F++D      R +++QYI+LI PD           T DG     P+++A  F +D   GHGTH A + AG+T+ +PAE ++C +G ++IGC GGCI               T S   +S A +  DI+RLC  FGC   S   CL ++ ++TL++ GG+A GAK+A  D VF  D++ G  AGNGLWE C +AGC++HSNSYG D  C LT  D+LYDE+MYEN EHLL+F+AGN G  T +D CT++SP IAKNVL VG+++SG TR+S T           + + ID ++ FS YGFT+DGRIKPEVVAPGD +YSA+ +G +   SC L A+AGTS S  + +GAAAM+RQYF D+ FY AD+++RGFC     C+ F P A T+KA + 
Sbjct:   31 EQTLLTTAGADFDSEA-AFAFASAWQAG----DRQGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSS-----WEDFTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAG-----RLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEE-EAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEH-GDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDS----DRRKVIQYIDLINPD-----------TGDGSSSGMPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCI---------------TESSTGSSEADYGEDINRLCPAFGC---SGDQCLPDDTADTLSEHGGMAQGAKIAAID-VFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS--SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLI 783          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 523 bits (1346), Expect = 9.310e-172
Identity = 287/516 (55.62%), Postives = 352/516 (68.22%), Query Frame = 1

Query:  901 MGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFES-----------------WTTSATF-GDNRATNKIFTFDDASV-DGRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAETIACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHAASHAFFDIDRLC---SDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            MGLVSF AS+ EV+RVAPR    L NAAARANIQSAT+T+TPLTDAGL+G GQV+QV+DSG+DESSCFFAHD +GD V HG+YF++ G N  +  S                 W T+    G      + F   D ++   R +I+QYINLIK D+T      SFTT  G+++PWL+A  FE D  AGHGTHVAGS AGATL TPAE I+C + + + CVG C++ +  A D+  DDL T     A     D+DRLC   SD+ C       CL ++V+ETLT +GG+A GAKLAIFDA +       F G GLWE C +A C++HSNSYG D EC L  +D+LYDE+M  NPE+LLIFAAGNDG   R  CT+ SPAIAKNVLAVGASTSG TR+SAT  +       GID +A FS+YG T D RIKPEV+APGD++YSAASDG  D +SCRL+A+ GTSMS PIVAGA+AMIRQYFM+  F+  D+ ARGFC +W GCE F PS+ATLKA + 
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDD-EANDV--DDLATPYVEIA-----DLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGS-------GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMI 501          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: D8LBZ2_ECTSI (Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBZ2_ECTSI)

HSP 1 Score: 514 bits (1324), Expect = 2.510e-170
Identity = 301/630 (47.78%), Postives = 397/630 (63.02%), Query Frame = 1

Query:  586 MSESLDLHSSNFWSH----------TTNKGGEHLATPAGALRGRDWSKAATVVHELSEA-AGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASVD-GRHRIVQYINLIKPD-------NTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAE------TIACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHA---ASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAG-GFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAG------IDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLK 2370
            MSESLD+H  +FWS                G+ L    G +  R+W++AA V+  L  A  G + G +CSWD + + H  +D+L + G+DHLL  G GAGG     EA EL VACFM L+SF ASR EV+RV+P   + L NAAA  N+QSA LTE+P+TDAGL+G G+VIQ+VD+GLD +SCFF  +  G+ V HG+YF+++G       SW TS  F  N    ++FT  D + D  R +IVQYINLI+ D       N       S+ T  G ++PW++A EF  + A GHGTH AGSAAGA + +P+       T  CE G+ + CVGGCI+ + + G    DDL+TT ++    A +   D+DRLC    C +   + CL ++ +ETL + GGVA GA+LAIFD +  +   G   AGNG+WE  +EAG +L SNS+G    C     D+LYD+FMY N + LL+FAAGNDG +    CT+ SPA+AKNVLAVGASTSG  R+S T   E    T G      ID +AYFS++G T DGRIKPEVVAPGD +YSAA  G+ + Y+CRL+ +AGTSMS P+VAGAAA++RQYF +  FY  D++ RGFC  + GCE F PSAAT+K
Sbjct:    1 MSESLDVHGKSFWSSGWSAKLLDEAAAEGAGDSLGGGPGEVLAREWTRAADVLRGLGGAEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGN-EGPEADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSANLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDF--NLQIARVFTGGDFNFDLSRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGDQYPWISAPEFAENYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGG---DDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMWEPAMEAGSKLSSNSWGIYAYCEYLSYDVLYDDFMYRNQDQLLLFAAGNDGEEW-SGCTMGSPAVAKNVLAVGASTSGPGRLSFTD--EDGDPTDGDSRVQDIDAMAYFSSFGPTLDGRIKPEVVAPGDMIYSAAGGGDGN-YTCRLWQYAGTSMSTPVVAGAAALVRQYFKNETFYTHDVEERGFCDSF-GCEPFAPSAATVK 619          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 528 bits (1359), Expect = 4.070e-169
Identity = 361/909 (39.71%), Postives = 480/909 (52.81%), Query Frame = 1

Query:    1 LQAVENVLLTTAQDDFDGGVEAFEAAARWSAERLLEDGDGRAPHLACTEYENSREVF-ERLNAFLSPRAV--RPASHSQRQGACFFATASHQEAATIQEQREAQFELIISFTIFPSALKLAPGLLQHGEEDDDDAEERMGRSEDMNAAAHGPDRLRARHGASMCRDT----------------------------------------------AEGLSVELTPGTLPA-HAPGADG---------FMSALHRGLMSESLDLHSSNFWSH----------TTNKGGEHLATPAGALRGRDWSKAATVVHELSEA-AGTSPGHICSWDSVAIYHPGNDVLLIAGLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASVDGRHRIVQYINLIKPD---------------------------NTPEELDASFTTRDGRKFPWLTAGEFENDKAAGHGTHVAGSAAGATLYTPAE------TIACENGKIIGCVGGCINQNASAGDLTDDDLVTTSEHA---ASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAG---GFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAG------IDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            L  +   LLTTA   F    E F+AA  W   RL  D    AP+LAC   ++ R      L+  L   A+  R    S   G C  ATA+  +A +I + ++    +  S+  FPSALKLAPGLL H  +D D                 G  RLR  HG  + R                                                  GL+VEL PG L A +  G+ G                 LMS+SLD+H  +FWS                G+      G +  R+W++AA V+  L     G + G +CSWD + + H  +D+L + G+DHLL  G GAGG     EA EL VACFM L+SF ASR EV+RV+P   + L NAAA  N+QSA+LTE+P+TDAGL+G G+VIQ+VD+GLD +SCFF  +  G+ V HGYYF+++G     F SW TS  F  N    ++FT  D + D   R    ++   P                            N       S+ T  G ++PW++A EF  D A GHGTH AGSAAGA + +P+       T  CE G+ + CVGGCI+ +   G+   DDL+TT ++    A +   D+DRLC    C +   + CL ++ +ETL + GGVA GA+LAIFD +  +D+ G     AGNG+WE  +EAG +L SNS+G    C     D+L+D+FMY N + LL+FAAGNDG+     CT+ +PA+AKNVLAVGASTSG  R+S T   E    T G      ID +AYFS++G T D RIKPEVVAPGD +YSAA  G+ + Y+CRL+ +AGTSMS PIVAGAAA++RQYF +  FY  D++ RGFC  + GCE F PSAAT+KA + 
Sbjct:   32 LSVIRGALLTTAGVTFRHQGE-FKAAVEWHEARL--DDARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQ----VFSSWAAFPSALKLAPGLLDHPHDDRD--------------GGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGN-EGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDF--NVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGE---DDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDII--NDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDW-SGCTMGTPALAKNVLAVGASTSGPGRLSFTD--EDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGN-YTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDSF-GCEPFAPSAATVKAMLI 906          

BLAST of mRNA_P-littoralis_Contig861.2.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 486 bits (1252), Expect = 4.310e-158
Identity = 286/531 (53.86%), Postives = 350/531 (65.91%), Query Frame = 1

Query:  814 GLDHLLYTGMGAGGKGHDGEAAELHVACFMGLVSFFASRVEVMRVAPRHKVGLTNAAARANIQSATLTETPLTDAGLNGAGQVIQVVDSGLDESSCFFAHDATGDQVTHGYYFDDLGFNYDIFESWTTSATFGDNRATNKIFTFDDASV-DGRHRIVQYINLIKPDNTPEELDASFTTRDGRKFPWLTAGEFENDKAA-----GHGTHVAGSAAGATLYTPAETIACENGKIIGCVGGCINQNASAGDLTD--DDLVTTSEHAASHAFFDIDRLCSDFGCGEESNKLCLRNNVSETLTKSGGVAPGAKLAIFDAVFKHDFAGGFAGNGLWEACVEAGCRLHSNSYGSDNECMLTPSDILYDEFMYENPEHLLIFAAGNDGNKTRQDCTVNSPAIAKNVLAVGASTSGSTRISATSAAEPSKDTAGIDEIAYFSAYGFTKDGRIKPEVVAPGDSVYSAASDGEEDKYSCRLFAFAGTSMSCPIVAGAAAMIRQYFMDAQFYGADLKARGFCGDWDGCEGFVPSAATLKAKIF 2382
            GLDHLL++G GAGG  H+ E+ ELHVACFMGLVS  A R EV+RV+PR    + NAAAR  +QSA+ T+TPLTDAGL+G G++IQV+DSGLDE+SC+F  D+ G++V HGYYFD+L     ++ S  +S    D  AT  IF   D S    R +I+QYI+L+ PD+       S  +  G   P    GE +  + A          V  SAAGATL  PAETI C     + CVG CI       DL D  DDLV+ S         D+DRLC  +GC   ++  CL ++V ETL+ +GG+A GAKLAIFD  +    A    GN LWE C EAGC+LHSNS G+D EC +   DIL D+FMYEN EHLLIFAAGN+G + R  CT+N+P I KN LA+GASTSG TR+ +T  A       GI+ +A FS++GFT D RIKPEVVAPGDSVYSAASDG  D +SCRL+A+ GTSMSCPIVAGAAAMIRQYFMD  FY AD+ ARGFC D   CEGF PSAAT+KA + 
Sbjct:    5 GLDHLLFSGRGAGGS-HEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDS-GEEVDHGYYFDELA-QAAVYSSLYSS----DETAT--IFNGGDFSYYPERRKIIQYIDLVNPDSPYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACI-------DLLDVTDDLVSISHQTGGT---DLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGAT------GINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGT-DTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLI 509