mRNA_P-littoralis_Contig756.15.1 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig756.15.1
Unique NamemRNA_P-littoralis_Contig756.15.1
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: D7G906_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G906_ECTSI)

HSP 1 Score: 139 bits (350), Expect = 2.450e-35
Identity = 85/134 (63.43%), Postives = 93/134 (69.40%), Query Frame = 2 
Query:  254 MLNAMSRNRWLC---AVTAVAE----ARAVLAKHFTPMVPPVLKRRPQQRFDFSSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTP 634
            M  A +RN  LC   AV A AE    A AV          P+ +RR       S+ S   RED IASILESK+EASKAVVHDISGGCGSM+KIEVESPLFRGKGLVAQHR+VK AL  EIADMHGLTLLTRP+P
Sbjct:    1 MWAAFARNPRLCVPAAVKACAEFPLRAIAVAGSDSRACYWPLEQRR-----GLSNGSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSP 129
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A7S2CEL3_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2CEL3_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 6.240e-20
Identity = 58/130 (44.62%), Postives = 78/130 (60.00%), Query Frame = 2 
Query:  251 VMLNAMSRNRWLCAVTAVAEARAVLAKHFTPMVPPV--LKRRPQQRFDFSSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTP 634
            VML A+ R+    A     +A  V+A    P +P +  +  RP        SS   +ED++   L + +E +  +V D+SGGCGSM+KIEVESPLFRG  LV QHR+V D LK EI +MHGLT+ TR +P
Sbjct:   10 VMLRAIGRSA--AAAIPARQAPCVVAPSSLPALPALGGVLHRPM-------SSEVPKEDLMKEALIAALEPTDVLVEDVSGGCGSMYKIEVESPLFRGVNLVKQHRMVTDTLKKEIGEMHGLTINTRRSP 130
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A836CI04_9STRA (Bola protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CI04_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 1.080e-17
Identity = 41/80 (51.25%), Postives = 58/80 (72.50%), Query Frame = 2 
Query:  395 SSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTP 634
            + + G++E  +  +L  +++A+K  V DISGGCGSM+ I +ESPLF+G  LV QH++VK  LK EIA MHGLTL T+ +P
Sbjct:   34 TEAAGSQEKAMEKLLGKELKATKVTVEDISGGCGSMYSIAIESPLFKGLSLVKQHQLVKSTLKKEIAAMHGLTLQTKVSP 113
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A7S2H125_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2H125_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 8.740e-17
Identity = 43/81 (53.09%), Postives = 55/81 (67.90%), Query Frame = 2 
Query:  392 SSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTP 634
            S S G ++E+ +   L   + AS   V DISGGCG+M+K+EVESP FRG  ++ QHR+V   LK EI +MHGLTL TR TP
Sbjct:   31 SESDGVSKEESMRLALSQALNASFVSVDDISGGCGAMYKLEVESPDFRGLNMLKQHRLVTQVLKKEIGEMHGLTLQTRVTP 111
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A3R7H807_9STRA (Uncharacterized protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7H807_9STRA)

HSP 1 Score: 85.1 bits (209), Expect = 2.950e-16
Identity = 46/89 (51.69%), Postives = 57/89 (64.04%), Query Frame = 2 
Query:  365 RRPQQRFDFSSSSGGT---REDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLT 622
            +RP  R  FSSS  GT    E  + S L +++EA    V D+SGGCGSMF IEV SP F+G+  V QHR+V + LK EI  MHGLT+ T
Sbjct:   14 KRPMSRAWFSSSGDGTGSEAEQAMHSALAARLEAVHVKVTDVSGGCGSMFDIEVASPQFKGQSRVKQHRMVNEILKEEIKSMHGLTIRT 102
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A4D9DBR1_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DBR1_9STRA)

HSP 1 Score: 85.1 bits (209), Expect = 4.470e-16
Identity = 39/79 (49.37%), Postives = 56/79 (70.89%), Query Frame = 2 
Query:  392 SSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRP 628
            +S+    RED + ++L    + +  ++ D+SGGCG+M+ +EVES LFRGK LVAQHR+V   L  EI++MHGL+L TRP
Sbjct:   45 NSAESVAREDRMRALLSEHFKPTHLLIQDVSGGCGAMYNMEVESELFRGKALVAQHRMVNQVLAKEISEMHGLSLKTRP 123
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A1V9Y5G4_9STRA (Uncharacterized protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Y5G4_9STRA)

HSP 1 Score: 84.0 bits (206), Expect = 5.510e-16
Identity = 47/83 (56.63%), Postives = 57/83 (68.67%), Query Frame = 2 
Query:  389 FSSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTPA 637
            FSS++    E+ +A  L  ++ A+   V DISGGCGSM+KIEV SP F GK +VAQHR+V   LK EIA MHGLTL T  TPA
Sbjct:   14 FSSAA----EEKMAVALRQELSATHVKVDDISGGCGSMYKIEVASPTFEGKNVVAQHRLVNQVLKEEIAGMHGLTLKTY-TPA 91
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A067C2D2_SAPPC (Uncharacterized protein n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067C2D2_SAPPC)

HSP 1 Score: 83.2 bits (204), Expect = 1.090e-15
Identity = 44/76 (57.89%), Postives = 52/76 (68.42%), Query Frame = 2 
Query:  410 TREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTPA 637
            T E+ +A+ L   + A+   V DISGGCGSM+KIEV SP F GK  VAQHR+V + LK EI  MHGLTL T  TPA
Sbjct:   21 TAEEKMATSLRQALSATHVKVDDISGGCGSMYKIEVASPTFEGKNRVAQHRLVNEVLKEEIGGMHGLTLKTY-TPA 95
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: C5K6X1_PERM5 (Uncharacterized protein n=1 Tax=Perkinsus marinus (strain ATCC 50983 / TXsc) TaxID=423536 RepID=C5K6X1_PERM5)

HSP 1 Score: 82.0 bits (201), Expect = 1.920e-15
Identity = 39/86 (45.35%), Postives = 57/86 (66.28%), Query Frame = 2 
Query:  377 QRFDFSSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTP 634
            +RF  S ++  T E  + S+L + + A+ A V D+SGGCGS +K+EV SP F G   + QH++V DALK EI   H +T++T+PTP
Sbjct:    3 RRFFCSPAATNTYEKSLESLLRASLGATSAKVQDVSGGCGSSYKVEVHSPKFIGLTRIKQHQMVNDALKDEIKKWHAITIITKPTP 88
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BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Match: A0A7S1PLD1_NEODS (Hypothetical protein n=1 Tax=Neobodo designis TaxID=312471 RepID=A0A7S1PLD1_NEODS)

HSP 1 Score: 80.9 bits (198), Expect = 4.890e-15
Identity = 38/79 (48.10%), Postives = 58/79 (73.42%), Query Frame = 2 
Query:  422 VIASILESKVEASKAV-------VHDISGGCGSMFKIEVESPLFRGKGLVAQHRIVKDALKAEIADMHGLTLLTRPTPA 637
            V A+ +ESK+ A+ A+       + DISGGCGS FK++VESP F+GK ++ QHR+V + LK EIA++HG+T++++  PA
Sbjct:   10 VTAADIESKLRAADALAPLRDVKIDDISGGCGSFFKVQVESPAFQGKSMLQQHRLVHEVLKKEIAEIHGITVVSKVPPA 88
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig756.15.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G906_ECTSI2.450e-3563.43Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S2CEL3_9STRA6.240e-2044.62Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A836CI04_9STRA1.080e-1751.25Bola protein n=1 Tax=Tribonema minus TaxID=303371 ... [more]
A0A7S2H125_9STRA8.740e-1753.09Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A3R7H807_9STRA2.950e-1651.69Uncharacterized protein n=3 Tax=Phytophthora kerno... [more]
A0A4D9DBR1_9STRA4.470e-1649.37Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A1V9Y5G4_9STRA5.510e-1656.63Uncharacterized protein n=1 Tax=Achlya hypogyna Ta... [more]
A0A067C2D2_SAPPC1.090e-1557.89Uncharacterized protein n=2 Tax=Saprolegnia TaxID=... [more]
C5K6X1_PERM51.920e-1545.35Uncharacterized protein n=1 Tax=Perkinsus marinus ... [more]
A0A7S1PLD1_NEODS4.890e-1548.10Hypothetical protein n=1 Tax=Neobodo designis TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig756contigP-littoralis_Contig756:53443..56124 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7G906
Preferred nameBOLA3
PFAMsBolA
Model size1833
Max annot lvl2759|Eukaryota
KEGG koko:K22075
Hectar predicted targeting categoryother localisation
GOsGO:0000981,GO:0003674,GO:0003700,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005759,GO:0006355,GO:0006357,GO:0006807,GO:0008150,GO:0008152,GO:0009889,GO:0010467,GO:0010468,GO:0010556,GO:0019219,GO:0019222,GO:0019538,GO:0031323,GO:0031326,GO:0031974,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044238,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050794,GO:0051171,GO:0051252,GO:0051604,GO:0060255,GO:0065007,GO:0070013,GO:0071704,GO:0080090,GO:0097428,GO:0106035,GO:0140110,GO:1901564,GO:1903506,GO:2000112,GO:2001141
Exons3
Evalue5.58e-40
EggNOG OGsCOG0271@1|root,KOG3348@2759|Eukaryota
Ec32 ortholog descriptionBolA protein
Ec32 orthologEc-15_002390.1
Descriptionprotein maturation by iron-sulfur cluster transfer
Cds size552
COG categoryT
BRITEko00000,ko03029
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791561.1174412-UTR-P-littoralis_Contig756:53442..546351680791561.1174412-UTR-P-littoralis_Contig756:53442..54635Pylaiella littoralis U1_48UTRP-littoralis_Contig756 53443..54635 -
1680791561.161724-UTR-P-littoralis_Contig756:56036..561241680791561.161724-UTR-P-littoralis_Contig756:56036..56124Pylaiella littoralis U1_48UTRP-littoralis_Contig756 56037..56124 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791561.131751-CDS-P-littoralis_Contig756:54635..548341680791561.131751-CDS-P-littoralis_Contig756:54635..54834Pylaiella littoralis U1_48CDSP-littoralis_Contig756 54636..54834 -
1680791561.1420789-CDS-P-littoralis_Contig756:55485..555601680791561.1420789-CDS-P-littoralis_Contig756:55485..55560Pylaiella littoralis U1_48CDSP-littoralis_Contig756 55486..55560 -
1680791561.1520884-CDS-P-littoralis_Contig756:55758..560361680791561.1520884-CDS-P-littoralis_Contig756:55758..56036Pylaiella littoralis U1_48CDSP-littoralis_Contig756 55759..56036 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig756.15.1prot_P-littoralis_Contig756.15.1Pylaiella littoralis U1_48polypeptideP-littoralis_Contig756 54636..56036 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig756.15.1

>prot_P-littoralis_Contig756.15.1 ID=prot_P-littoralis_Contig756.15.1|Name=mRNA_P-littoralis_Contig756.15.1|organism=Pylaiella littoralis U1_48|type=polypeptide|length=184bp
MYRCSIHVSDPPNRYSIAVVRRHLNCSHHFRFPVRAPPSPSATPTTAVLL
RIKTVMLNAMSRNRWLCAVTAVAEARAVLAKHFTPMVPPVLKRRPQQRFD
FSSSSGGTREDVIASILESKVEASKAVVHDISGGCGSMFKIEVESPLFRG
KGLVAQHRIVKDALKAEIADMHGLTLLTRPTPA*
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mRNA from alignment at P-littoralis_Contig756:53443..56124-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig756.15.1 ID=mRNA_P-littoralis_Contig756.15.1|Name=mRNA_P-littoralis_Contig756.15.1|organism=Pylaiella littoralis U1_48|type=mRNA|length=2682bp|location=Sequence derived from alignment at P-littoralis_Contig756:53443..56124- (Pylaiella littoralis U1_48)
CACTAATAGCTGCTGTATTCTAATTGGCCAAGATTCTCTTGAGACCAAAC
GTCCCTCACGCTCCCCGGGCTGTGTGGTACATGTACACATGTACCGTTGT
AGTATACACGTATCTGATCCTCCCAACCGATACAGCATTGCAGTAGTACG
TCGTCACCTCAACTGCTCTCATCACTTCCGTTTCCCCGTTCGAGCTCCTC
CTTCTCCTTCTGCTACTCCTACTACCGCTGTTCTACTCCGGATCAAAACG
GTGATGTTGAACGCCATGTCTAGAAATCGCTGGCTTTGCGCAGTGACAGC
AGTGGCAGAGGCGCGCGCCGTGCTGGCAAAACATTTTACTCCCATGGTTC
CACCGGTGTTGAAGAGGTGAGATGTTTGGTCGACCTTCTGCTGTTACTGC
TGTATGCAACGACGAAACCGAGGATGGCTTGCAGCTTACTCCTACACTTC
ATGCACCAGGGACGTGGCTCGCACCGAAACGTGAAGTTTGATGGAAGTGC
GCAAAGTGATTCCCACTCAGTCTACTGTTAAATCCTTGCTTTTTCATTCT
ACATCATATGATAGGCGACCCCAGCAACGATTCGACTTCAGCAGCAGCTC
GGGCGGCACGAGAGAAGACGTGATCGCCTCAATCCTCGAGTAAGTGCGGA
ACACACGACCTGTCGGTACTGCAGTGAAGCACGATTCGAACTTGTCCATT
TGTTCGGAAAATTCCAGGAAGAATGAAAGTGGATGTGTTTGTTTATCTGT
CGAAGAAGCGAATTCCAAATTCGCAGAAATTGTCGTGAGCATGTCAATCT
GGTGTACTGGTTCTTTACACTACGTACATACCACACAGCAGTTTTACATG
CCGACGGCAAAGTTGCATCGAGTTCGTCGTGTGTTGAGGCAGCGTGTACA
CCGAGGAGCCGCCAAGTTGGGTCGTCGGTACTGCGGTACAGGTTTTATTC
CCAGATTTTAATATATGATACAGGACAGCAGTATTACTTCGAAGTACTAG
TACAAGAACGCCAACAACGTCGCAGAGCTACGTTGTTTCTTCGGTTCCTG
GTGCAGCTGTACACGGTTCGACTCCAACATCAACTGCTTGGCGCAGGCGC
GCGTGGAGAAGGGGGGGGGGGACGCGCATTATGACGGGTAGAAAAAGGAA
TCACAACCTCATCACTCGCACCTCGAAATCGGTAACTTTGTTGTGCAGCT
GGGGGAAGATACCACACAAGCTCTAAAAACTGGGTCAAGCGCGAATAGAA
AGTGAGTCACCCTGCTGTGGCCTTGTCTCCTCGCACGCAGGTCGAAGGTC
GAGGCATCGAAAGCTGTCGTGCATGACATATCAGGAGGCTGCGGGAGCAT
GTTCAAAATCGAGGTTGAGTCCCCATTGTTTCGAGGGAAGGGGCTGGTGG
CGCAACACCGTATCGTGAAGGACGCTCTGAAGGCCGAGATCGCAGACATG
CACGGGTTGACGCTGCTCACACGGCCAACACCAGCGTAGGGTATAGTCAA
AGAGTAGGAATGTTCTCGTTGGGGTCACGACATCGACGACAGCAGTATTT
TCGAAAACAATTTGACTAAAATGGCTTCCACACTTCGAAGTATATTTTTA
AGTTTTTTCGTGATACATTTTACGGCGATGTTTTGCGTTTTCCGTTAACG
CAGAACCCGACCTGTCGTTTGCGTTTTCCCCCGTAGAGACACCAAGATTC
TCATACTCTGTATCGGCTCGATGATGCGGGGAGGGTCCGGCAGCTCTGCA
AGCGATATAACTGCATGACTCAAAAATTAATCTCGGGGGACAGACACCCA
TTCGATGGAGTGTTTAATCGGCCGCTTTGGCAGCGAGTTACGTTCATCAA
TGAGATACGAAGTATTGTGTACGGCTTCGCTGTATACTTCGAAGTGTATT
GTGTGGATGGAAGCGTTCCACGGCGGTACTGGACCGCAACAAACCCAGCC
CAGATGATTGTTCCTCGTATTAATGTGTGTCAGAGGATGACAAAGTGCAC
GCAGTTGACCAAAAGGAAAACTGCGAGCACTTACTTACACGTAAACATGT
CTCCTTTTGAGTAAAAACTTCGCGCGTGAAACGTGGAACTCGGACTTCTC
GTTCCTTGTTCCATGCGTTGTGTAGCTTCTTCCTCACCAGCAGCCCGTTC
CACATGGACTGTTGGACAGCAGTATTAACGTGTTTGCGCAGAGCAAATTT
TAATGTCGGCAGCTAACGACCTTCGGAGTATTCAAAAGTTCTCCTTCCAA
TTTTTGATTGAATTTTTTCTTCATAATGGGCTTATGTGTAGAAAGATTAA
GATTTGATAGCTAAATTTGACACCCTAGCGACCAGTGATGCTGGTTGGCC
CACGTCGGCTGGGCGGACTGTTTGAGGTCCGGCACTCAGTAGTACAGAGT
TACAGGGTGTTCGCAGACAACAGGCGCTCGTGCGTGTACCCCACTACATG
ACAGACGGCTCCCTTGTTCAAATATCAATATCGACCGTTAAATCATGGCA
ACAAGAAGGGCTACTGCCGCTGTTGTATCGGATAAGTGGATCTCAAGCGT
TGCTACGGCGCCATGGCCACAGAGAGGAGAGTTCATAACCGAGTGCCAAC
CATGGCTTGTCATGCTTCGCAACCCCACATACTCATTATTTAATGTGGGG
CTGAGCTAGACAGATAAAAGACTGGGAAGGAC
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Coding sequence (CDS) from alignment at P-littoralis_Contig756:53443..56124-

>mRNA_P-littoralis_Contig756.15.1 ID=mRNA_P-littoralis_Contig756.15.1|Name=mRNA_P-littoralis_Contig756.15.1|organism=Pylaiella littoralis U1_48|type=CDS|length=552bp|location=Sequence derived from alignment at P-littoralis_Contig756:53443..56124- (Pylaiella littoralis U1_48)
ATGTACCGTTGTAGTATACACGTATCTGATCCTCCCAACCGATACAGCAT
TGCAGTAGTACGTCGTCACCTCAACTGCTCTCATCACTTCCGTTTCCCCG
TTCGAGCTCCTCCTTCTCCTTCTGCTACTCCTACTACCGCTGTTCTACTC
CGGATCAAAACGGTGATGTTGAACGCCATGTCTAGAAATCGCTGGCTTTG
CGCAGTGACAGCAGTGGCAGAGGCGCGCGCCGTGCTGGCAAAACATTTTA
CTCCCATGGTTCCACCGGTGTTGAAGAGGCGACCCCAGCAACGATTCGAC
TTCAGCAGCAGCTCGGGCGGCACGAGAGAAGACGTGATCGCCTCAATCCT
CGAGTCGAAGGTCGAGGCATCGAAAGCTGTCGTGCATGACATATCAGGAG
GCTGCGGGAGCATGTTCAAAATCGAGGTTGAGTCCCCATTGTTTCGAGGG
AAGGGGCTGGTGGCGCAACACCGTATCGTGAAGGACGCTCTGAAGGCCGA
GATCGCAGACATGCACGGGTTGACGCTGCTCACACGGCCAACACCAGCGT
AG
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