mRNA_P-fluviatile_contig1.466.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: D8LNF6_ECTSI (Sterol delta-7 reductase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNF6_ECTSI)

HSP 1 Score: 806 bits (2083), Expect = 2.040e-283
Identity = 377/466 (80.90%), Postives = 414/466 (88.84%), Query Frame = 1

Query:  808 KTMAPKTPAKNAN--MNIKDAVKASVAAESQDSKLWGFVPNWFRTSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLFNADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGVL 2199
            ++MA +T  K A+  M+I++AVKASVAAES+DSKLWGFVPNWFRT+VGPLFLIL PP+FVV +W++LV   GS  ++W ++      +   ++PSPVDP AWKYIL F  FEI LMVGLPGK+FRANPTATGH+PVYKANGM SY+VTLATLC LV    LDPK+VYDKLGEIF GLS FSLFFVL LT KGLYFPSTDDSGSNGSFLQNYWWGTELYPR+F ADVKMFTNCRFGMMYWAVG+VIYAY QQ MYG LS+SMAVSVILQLTYITKFFHWEMGYMN+MDIQHDRAG+YLCWGCLVWVPAVYSSPGIYLVKHPI LG YG +AIL LGLLSIWAN+DADRQRHAFRQAKG I VWGKPA+YITAGY+NARGEKASSLLL TGWWGVARHFHYLPEITGAFFWTVPALFE+  PYFYVVFL+LLLTDRAFRDD RCRGKYGKHWDKYCA+VPYKIVPG+L
Sbjct:   47 RSMALRTSTKQASDAMDIREAVKASVAAESKDSKLWGFVPNWFRTTVGPLFLILVPPFFVVFWWHLLVSHSGSWVSLWGDLKAAGPEYVLDVVPSPVDPAAWKYILGFGVFEILLMVGLPGKAFRANPTATGHIPVYKANGMLSYLVTLATLCALVATDRLDPKNVYDKLGEIFTGLSVFSLFFVLLLTVKGLYFPSTDDSGSNGSFLQNYWWGTELYPRVFGADVKMFTNCRFGMMYWAVGAVIYAYTQQQMYGKLSSSMAVSVILQLTYITKFFHWEMGYMNSMDIQHDRAGYYLCWGCLVWVPAVYSSPGIYLVKHPISLGWYGASAILALGLLSIWANFDADRQRHAFRQAKGDIIVWGKPAKYITAGYINARGEKASSLLLCTGWWGVARHFHYLPEITGAFFWTVPALFETPTPYFYVVFLVLLLTDRAFRDDTRCRGKYGKHWDKYCAQVPYKIVPGIL 512          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A6H5JQU0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQU0_9PHAE)

HSP 1 Score: 592 bits (1525), Expect = 1.030e-201
Identity = 279/354 (78.81%), Postives = 309/354 (87.29%), Query Frame = 1

Query:  814 MAPKTPAKNAN--MNIKDAVKASVAAESQDSKLWGFVPNWFRTSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLFNADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAK 1869
            MAP+T  K A+  M+I++AVKASVAAES+DSKLWGFVPNWFRT+VGPLFLIL PP+FVV +W++LV   GS  ++W ++      +   ++PSPVDP AWKYIL F  FEI LMVGLPGK+FRANPTATGH+PVYKANGM SY+VTLATLC LV    LDPK+VYDKLGEIF GLS FSLFFVL LT KGLYFPSTDDSGSNGSFLQNYWWGTELYPR+F ADVKMFTNCRFGMMYWAVG+VIYAY QQ MYG LS+SMAVSVILQLTYITKFFHWEMGYMN+MDIQHDRAG+YLCWGCLVWVPAVYSSPGIYLVKHPI LG YG +AIL LGLLSIWAN+DADRQRHAFRQA+
Sbjct:    1 MAPRTSTKQASDAMDIREAVKASVAAESRDSKLWGFVPNWFRTTVGPLFLILVPPFFVVFWWHLLVSHSGSWVSLWGDLKAAGPEYVLDVVPSPVDPEAWKYILGFGVFEIVLMVGLPGKAFRANPTATGHIPVYKANGMLSYLVTLATLCALVATDRLDPKNVYDKLGEIFTGLSVFSLFFVLLLTVKGLYFPSTDDSGSNGSFLQNYWWGTELYPRVFGADVKMFTNCRFGMMYWAVGAVIYAYTQQQMYGKLSSSMAVSVILQLTYITKFFHWEMGYMNSMDIQHDRAGYYLCWGCLVWVPAVYSSPGIYLVKHPISLGWYGASAILALGLLSIWANFDADRQRHAFRQAR 354          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A835YQ61_9STRA (Sterol delta-7 reductase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQ61_9STRA)

HSP 1 Score: 558 bits (1438), Expect = 3.880e-187
Identity = 269/452 (59.51%), Postives = 329/452 (72.79%), Query Frame = 1

Query:  847 MNIKDAVKASVAAESQDSKLWGFVPNWFRTSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSF-LQNYWWGTELYPRLFNADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGVL 2199
            M+  D+ K SV   S++ K  G +P   R  +GPL LI   P FV++ W+ +  L+GS   +  E+      + + I PSPVD  AWK I  +AAFE+ALM  +PG+ F A  TATGHVPVYKANGM S+ +TLA    LV AG  D   VYDKLGEI +GL  FSL F  AL FKGLY PST DSG+NG+  L++Y+WGTELYPR+   DVK FTNCRFGMM+WAVG + YA+ Q   +G++S+SMAVSV+LQL YI KFFHWEMGYM TMDIQHDRAG+Y+CWGCLVWVP+VY+S G+YL +HP+ LG     AI   G+  IWANYDADRQR  FR   G   +WG+PAE I A Y N RG  + SLLL +GWWG++RHFHY+ EI+ AF W+VPALF +AAPYFYVVFL++LL DRA+RDD+RCRGKYGK WD+YC RVPYKI+PGVL
Sbjct:    1 MSSDDSNKRSVGFTSENGKFLGVLPESSRDYLGPLVLIAVCPAFVIVLWHTICNLDGSFLLLLKELKANGPGYIRDIWPSPVDGEAWKLIGSYAAFELALMRIVPGRRFEATATATGHVPVYKANGMASFAITLAAYALLVAAGW-DSGVVYDKLGEILSGLCVFSLAFCAALYFKGLYAPSTRDSGTNGAGPLKDYFWGTELYPRVLGWDVKQFTNCRFGMMFWAVGLLCYAHRQAREHGAVSDSMAVSVLLQLVYIAKFFHWEMGYMCTMDIQHDRAGYYICWGCLVWVPSVYTSAGMYLTRHPVHLGAPLALAITAAGIACIWANYDADRQRQHFRAVGGRCAIWGRPAEAIEATYDNGRGGTSRSLLLCSGWWGLSRHFHYVTEISSAFLWSVPALFHNAAPYFYVVFLVILLVDRAYRDDVRCRGKYGKFWDQYCQRVPYKIIPGVL 451          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A7S2XXP5_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XXP5_9STRA)

HSP 1 Score: 485 bits (1248), Expect = 1.020e-158
Identity = 226/422 (53.55%), Postives = 290/422 (68.72%), Query Frame = 1

Query:  937 SVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAF--FKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLFNADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGV 2196
             +G + L+L  P  + L WY    + GSL A+W+    E G F    + IP P DP  W+ +  +   E+A+M  +PGK+F A  T TGH PVYKANG+  Y ++L  L GL +AG+++P  +YD+ GE  + +  FSL F L L FKGLYFPST DSGS+G+FL +Y+ GTELYPR+   D+K+FTNCRFGMM+WA+G + +A+ Q    G +++SM V+V LQL YITKFFHWEMGYM TMDIQHDRAGFYLC+GCL ++P VY+S   YLVKHP+DLG      I   G+LS+W NYDADRQR  FR   G   VWGK  + + A Y    G+  +SLLL +GWWGV+RHFHY+PEI  +FFW +P+LF    PYFYV +L +LL  RA RDD+RC  KYGK W+KYC  VPYK+VPGV
Sbjct:   31 DIGSIALLLICPPCLQLLWYTSYAMNGSLLALWNSFF-EEGFFAVLDKAIPRPTDPFIWQVLFAYMTIELAMMRLVPGKNFTATITKTGHQPVYKANGLQCYFISLVGLLGLTYAGVINPSIIYDRFGEFMSAIQSFSLLFCLFLYFKGLYFPSTKDSGSSGNFLVDYFAGTELYPRILGFDIKLFTNCRFGMMFWALGIICFAHKQYMDLGYVTDSMMVNVALQLLYITKFFHWEMGYMCTMDIQHDRAGFYLCYGCLSYLPCVYTSHTYYLVKHPLDLGLPLALGIFAAGVLSVWINYDADRQRTEFRATSGKAKVWGKEPKMVEANYTTTDGKHRTSLLLASGWWGVSRHFHYIPEILASFFWALPSLFNHYLPYFYVTYLTILLMHRALRDDLRCGQKYGKDWNKYCKLVPYKVVPGV 451          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: W7TQE9_9STRA (7-dehydrocholesterol reductase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQE9_9STRA)

HSP 1 Score: 481 bits (1238), Expect = 2.480e-157
Identity = 234/445 (52.58%), Postives = 301/445 (67.64%), Query Frame = 1

Query:  886 ESQDSKLWGFVPNWFRTSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGM---LDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLFNADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHP-IDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAA---PYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGVL 2199
            ++++ K  G +P   R S+GPLFL+   P F+ + W+ +  L+G    +          + K I+PSP DP AWK IL + A E+A M  LPGK+F+A  T  G+VPVY+ANGM +Y  +L     L   G    L    VY  +GE+ + ++ FSL F L L  KGL FPS+ DSGS+G+++ +++WGTELYPR+   D+KMFTNCRFGMM+WA+G + YA AQ    G LSN+M VSV+LQL YITKFF+WE GY+ +MDIQHDRAG+Y+CWGCLVWVPAVY+SP  +LV H   D+G+     +L  G   +  NY ADRQR  FR + G  ++WGKP  +ITA Y   +GEK SS+LL +GWWGVARHFHY+PEI GAF W+ PA F        YFYV+FL  LL DRAFRDD RCR KYGKHWDKYCA VPYK++PG+L
Sbjct:    3 QNKNGKSSGLLPG--RESLGPLFLMGTTPIFIFITWFTMQHLDGDFGRLIESFRAHGWGYLKIIVPSPFDPTAWKVILSYMAVELAFMRLLPGKTFKATVTPAGNVPVYRANGMQAYAASLLLFFLLQRYGPAHGLHVSWVYHHMGELLSAMNVFSLAFCLFLLVKGLTFPSSSDSGSSGNWVIDFYWGTELYPRVLGFDLKMFTNCRFGMMFWALGILCYAQAQAEKDGFLSNAMLVSVLLQLIYITKFFYWETGYLCSMDIQHDRAGYYICWGCLVWVPAVYTSPSYFLVNHGGRDIGSLTALLLLSAGAACVGINYWADRQRQVFRASDGKCSIWGKPPVFITASYTTEKGEKRSSILLASGWWGVARHFHYVPEIMGAFLWSCPAGFYGLRFFLAYFYVIFLTPLLFDRAFRDDARCRDKYGKHWDKYCALVPYKMIPGIL 445          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A7S4DW51_9EUKA (Hypothetical protein n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S4DW51_9EUKA)

HSP 1 Score: 479 bits (1233), Expect = 1.580e-156
Identity = 226/429 (52.68%), Postives = 288/429 (67.13%), Query Frame = 1

Query:  916 VPNWFRTSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFF----KRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLFNADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVP 2190
            +P W R  +GP+FL+ + P F+++  +   +  GSL      M            K +  SP+DP AWK I  F  FE+ LM  +PGK FRA  T+TGH+P+YKANG+    +++ T   L +  ++ P +VYDK GEI A L+ F+L F   LTFKG YFPST DSGS G+ + +++WGTELYPR+   D+K FTNCRFGMMYWA+G + YA+ Q  MYG +S+SM VSV+LQL Y+TKFF WE GY  +MDIQHDRAG+Y+CWGCL WVPAVY+S   YLV HP+ LG      IL +G+L IW NYD+DRQR AFR+      +WG+  + I A Y    G    S LL +GWWG+ARHFHYLPE+  AF W+VPALF +  PYFYV+FL +LL DRA RDD RC  KY K+WD YC +VPYKIVP
Sbjct:   19 LPGWIREIMGPMFLMTSTPMFIMVVVHTCHKFGGSLLECLDYMFKNPAWMLPFSKKSMWMSPIDPEAWKMIGSFMIFELMLMRIVPGKEFRATMTSTGHIPIYKANGVQCLFISIITFIALGYCDIIQPGEVYDKFGEILAALNLFALIFCTFLTFKGHYFPSTADSGSTGNTVLDFYWGTELYPRVLGWDLKQFTNCRFGMMYWAIGPIAYAWKQYEMYGHVSDSMIVSVVLQLVYVTKFFWWETGYFCSMDIQHDRAGYYICWGCLCWVPAVYTSQSFYLVAHPVQLGRPLALTILLVGILMIWINYDSDRQRQAFRRNTKE-KIWGRTPDKIDAQYTTTDGSVRHSALLASGWWGLARHFHYLPEVFAAFCWSVPALFHNFLPYFYVIFLTMLLIDRANRDDARCACKYTKYWDMYCNKVPYKIVP 446          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A7J6IB75_CANSA (Uncharacterized protein n=1 Tax=Cannabis sativa TaxID=3483 RepID=A0A7J6IB75_CANSA)

HSP 1 Score: 476 bits (1225), Expect = 1.470e-155
Identity = 231/415 (55.66%), Postives = 285/415 (68.67%), Query Frame = 1

Query:  955 LILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLF-NADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGV 2196
            L L PP FV+L WY +V  +GS+   +  +       F  I P P    AWK I C+AAFE AL + LPGK      + TGH PVYKANGM +Y VTL T  GL   G+ +P  VYD LGEI++ L F SL F + L  KG   PS+ DSGS+G+ + +++WG ELYPR+  N D+K+FTNCRFGMM WAV +V Y   Q  + G +++SM V+ IL L Y+TKFF WE GY +TMDI HDRAGFY+CWGCLVWVP+VY+SPG+YLV HP+ LGT     IL  G+L I+ NYD DRQR  FR+  G   VWGK    ITA Y    GE  SS+LL +GWWG+ARHFHY+PEI+ AFFWTVPALF+ A PYFYVVFL +LL DRA RDD RCR KYGK+W  YC +VPY+++PG+
Sbjct:   21 LSLCPP-FVILLWYTMVHADGSIYQTFYYLKQHGLQGFIDIWPRPTA-TAWKLIACYAAFEAALQLLLPGKRVEGPISPTGHRPVYKANGMAAYFVTLTTYIGLWWFGIFNPSTVYDHLGEIYSALIFGSLVFCVLLYIKGHVAPSSSDSGSSGNIIIDFYWGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEVNGKVADSMLVNTILMLVYVTKFFWWEAGYWSTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLALYILVAGILCIYINYDCDRQRQEFRRTNGKALVWGKAPSKITASYTTTTGETKSSILLTSGWWGLARHFHYVPEISAAFFWTVPALFDHALPYFYVVFLTILLLDRAKRDDDRCRSKYGKYWKVYCEKVPYRVIPGI 433          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A7N0ZXD7_KALFE (Uncharacterized protein n=1 Tax=Kalanchoe fedtschenkoi TaxID=63787 RepID=A0A7N0ZXD7_KALFE)

HSP 1 Score: 475 bits (1223), Expect = 2.910e-155
Identity = 230/422 (54.50%), Postives = 285/422 (67.54%), Query Frame = 1

Query:  934 TSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLF-NADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGV 2196
            T V  L L+   P FV+L WY +V  +GS+   +  + +     F  I P P   +AWK I C+AAFE AL + LPGK      +  G+ PVYKANG+ +Y VTL T  GL   G+ DP  VYD LGEIF+ L F S  F + L  KG   PS+ DSGS G+ + +++WG ELYPR+  + D+K+FTNCRFGMM WAV +V Y   Q  +YG +++SM V+ +L L Y+TKFF WE GY NTMDI HDRAGFY+CWGCLVWVP+VY+SPG+YLV HP++LGT     IL  G+L I+ NYD DRQR  FR+  G  T+WGK    I A Y    GE  SSLLL +GWWG ARHFHYLPEI+ +FFWTVPALF    PYFYV+FL +LL DRA RDD RCR KYGK+W  YC +VPYKI+PG+
Sbjct:   13 TYVSMLSLLTLCPPFVILLWYTMVHADGSVLQTFDYLKSNGLQGFINIWPRP-SVLAWKIIGCYAAFEAALQLLLPGKRVEGPISPAGNRPVYKANGLQAYAVTLITYIGLWWFGIFDPSIVYDHLGEIFSALIFGSFVFCVLLYLKGHIAPSSTDSGSTGNLIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYELYGKVADSMLVNTVLMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCTIWGKAPSKIAASYTTTTGETKSSLLLTSGWWGQARHFHYLPEISASFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCEKVPYKIIPGI 433          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: Q6S5A3_TROMA (Sterol delta-7 reductase n=1 Tax=Tropaeolum majus TaxID=4020 RepID=Q6S5A3_TROMA)

HSP 1 Score: 475 bits (1223), Expect = 3.010e-155
Identity = 231/415 (55.66%), Postives = 280/415 (67.47%), Query Frame = 1

Query:  955 LILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAFFKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLF-NADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGV 2196
            L L PP FV+L WY +V  +GS+   W  +       F  I P P   +AWK I C+ AFE AL + LPGK      +  G+ P+YKANG+ ++ VTL T  GL   G+ +P  VYD LGEIF+ L F S  F + L  KG   PS+ DSGS G+ + +++WG ELYPR+  N D+K+FTNCRFGMM WAV +V Y   Q  + G +++SM VS IL L Y+TKFF WE GY NTMDI HDRAGFY+CWGCLVWVP++Y+SPG+YLV HP+DLGT     IL  G+L I+ NYD DRQR  FR+  G  TVWGK    I A Y    GE  SSLLL +GWWG+ARHFHY+PEI  AFFWTVPALF    PYFYVVFL +LL DRA RDD RCR KYGK+W  YC RVPYKI+PG+
Sbjct:   22 LSLCPP-FVILLWYTMVHADGSIGQTWDYLRQNGLQGFIDIWPRPTV-IAWKIIFCYGAFEAALQLLLPGKRVEGPISPAGNRPIYKANGLAAFAVTLITYLGLWWYGIFNPTIVYDHLGEIFSALIFGSFIFCIFLYLKGHLAPSSSDSGSCGNLIIDFYWGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYELNGRVADSMLVSTILMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSIYTSPGMYLVNHPVDLGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCTVWGKSPSKILASYTTTSGETKSSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFNHVLPYFYVVFLTILLFDRAKRDDDRCRSKYGKYWKLYCQRVPYKIIPGI 434          

BLAST of mRNA_P-fluviatile_contig1.466.1 vs. uniprot
Match: A0A251T2W8_HELAN (Putative 7-dehydrocholesterol reductase n=8 Tax=Asteraceae TaxID=4210 RepID=A0A251T2W8_HELAN)

HSP 1 Score: 474 bits (1220), Expect = 1.190e-154
Identity = 232/441 (52.61%), Postives = 297/441 (67.35%), Query Frame = 1

Query:  883 AESQDSKLWGFVPNWFRTSVGPLFLILAPPYFVVLFWYILVELEGSLTAVWSEMCTERGAF--FKRIIPSPVDPVAWKYILCFAAFEIALMVGLPGKSFRANPTATGHVPVYKANGMFSYVVTLATLCGLVHAGMLDPKDVYDKLGEIFAGLSFFSLFFVLALTFKGLYFPSTDDSGSNGSFLQNYWWGTELYPRLF-NADVKMFTNCRFGMMYWAVGSVIYAYAQQAMYGSLSNSMAVSVILQLTYITKFFHWEMGYMNTMDIQHDRAGFYLCWGCLVWVPAVYSSPGIYLVKHPIDLGTYGTAAILCLGLLSIWANYDADRQRHAFRQAKGHITVWGKPAEYITAGYVNARGEKASSLLLYTGWWGVARHFHYLPEITGAFFWTVPALFESAAPYFYVVFLILLLTDRAFRDDIRCRGKYGKHWDKYCARVPYKIVPGV 2196
            AES   K    V +   T +  + L+   P FV+L WY +V  +GS+   ++ +  E G    F  I P P   VAWK I C+A FE AL + LPGK+     + TG+ PVYKANG+ +YVVTL T   L   G+ +P  VYD LGEI++ L F SL F + L  KG   PS+ DSGS+G+ + +++WG ELYPR+  N D+K+FTNCRFGMM WAV +V Y   Q    G +++SM V+ +L L Y+TKFF WE GY NTMDI HDRAGFY+CWGCLVWVP++Y+SPG+YLV HP++LG     +IL  G+L I+ NYD DRQR  FR+  G   VWGK    I A Y   +GE  +SLLL +GWWG++RHFHY+PEI  AFFWTVPALF++  PYFYVVFL +LL DRA RDD RCR KYGK+W  YCA+VPY+I+PG+
Sbjct:    6 AESMKKKKEEMVHSPILTYLSVITLLSTCPPFVILLWYTMVHQDGSILNTFNYL-KEHGVVEGFVNIWPRPTA-VAWKIIACYALFEAALQLWLPGKTVEGPISPTGNKPVYKANGVLAYVVTLVTYISLWWFGIFNPSVVYDHLGEIYSALIFGSLVFCVFLYIKGHVAPSSTDSGSSGNIIFDFYWGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYETNGKVADSMLVNTVLMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSIYTSPGMYLVNHPVNLGPQLAVSILIAGILCIYINYDCDRQRQEFRRTNGKSLVWGKAPSKIVASYTTTKGETKTSLLLTSGWWGLSRHFHYVPEIMAAFFWTVPALFDNFLPYFYVVFLTILLFDRAKRDDDRCRSKYGKYWKTYCAKVPYRIIPGI 444