mRNA_P-fluviatile_contig18.3256.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: D8LF74_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LF74_ECTSI)

HSP 1 Score: 733 bits (1893), Expect = 5.630e-249
Identity = 423/656 (64.48%), Postives = 472/656 (71.95%), Query Frame = 1

Query:  472 AAPSVTSGEHAFAAGGAEQVAELLAGEQDGEGAVFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKRSPETLSTQPSPVDG-----------FEHRGAARRRRRWGLKAVLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGAGNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAP---LPVGSQREAN-------TSGGG-GADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNPQPPPVRAAGLAILGRGTQLITRADFGAGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLARRSVMARDEGETEGLE 2373
            AA +V   E AFA GGAE+VAELLAGE  GEGAVF+NRVGA+NRDLSVLMANVLAEER +ERL GKKRKKR     S +PSP DG               G ARR + WG++A +K A  R S                            DGL++LDAFAASGVRALRYLTEVP V+RVVANDYD AAI  IR+AA   G G+KL V+RGDA+DT+Y+ARAGG+GTYDL+DIDPFGCAAGFLDA VQAVTDGGLLCVTSTDMPVLSGAQPEVAW RYGSVPTRSGYHHEMSLRILL A + AAGR RRSI+PVLSVA+DHYVRVFVRVSRS K AL AAQE  SYVLQSE+CPSFFL PVL  +PK+        R R+     +ST P   LP G+  E+        TSG G G D      ++   P    GVC ETG  + AGGPIWSGPLH+E+WV RAIA+ASD G  +   D     VAVPRPRL A AR  S+LRAVSREL DVPLFYNLRDMFATLG K+HPRREQ+MGALE+AGYR SGMHKEPLA+KT+APDAAVWDVLRCWARDNPQPPPVR AG+AIL R  Q I  ADF A R G    R  G A   GGFLH HNPEQ+WGPLAR SVMAR + E +G+E
Sbjct:   69 AATAVGCSEEAFATGGAERVAELLAGESAGEGAVFYNRVGAINRDLSVLMANVLAEERVRERLAGKKRKKRQIPPTS-RPSPTDGTLRSRSSLAHDMAQSGEARRSKVWGVRAFVKGALGRLS---RGCWGVGVERMSNNKDQQDEPREDEDGLLILDAFAASGVRALRYLTEVPGVQRVVANDYDEAAINTIRQAAVQCGVGSKLEVHRGDALDTLYSARAGGIGTYDLIDIDPFGCAAGFLDASVQAVTDGGLLCVTSTDMPVLSGAQPEVAWTRYGSVPTRSGYHHEMSLRILLHAINTAAGRQRRSIQPVLSVAIDHYVRVFVRVSRSPKAALAAAQESTSYVLQSESCPSFFLRPVL--SPKQQSPHIKTERSRKARRLSDSTDPSTALPAGAPPESKEQELERVTSGAGLGVD------TQQSLPQGLGGVCPETGAGLMAGGPIWSGPLHDESWVTRAIAIASD-GEKSLGGDGADSRVAVPRPRLAARARAESLLRAVSRELLDVPLFYNLRDMFATLGLKKHPRREQVMGALEAAGYRTSGMHKEPLAVKTNAPDAAVWDVLRCWARDNPQPPPVREAGVAILRREMQSIGDADFDARRAGPGGSRREGVARGGGGFLHAHNPEQDWGPLARHSVMARVDDEQDGVE 711          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: D8LH70_ECTSI (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LH70_ECTSI)

HSP 1 Score: 297 bits (760), Expect = 9.980e-85
Identity = 212/602 (35.22%), Postives = 294/602 (48.84%), Query Frame = 1

Query:  571 VFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKRSPETLSTQPSPVDGFEHRGAARRRRRWGLKAVLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGAG-NKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNPQPPPVRAA--GLAILGRGTQLITRADFGAGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLARRSVMARDEGETEG 2367
            VF+N+V   NRDLS+ +  + AE+R +E+     RK R  E        ++G +          W  +A    +T                                DG+ +LDA AA+ +R++RY+ E+P V+ VV ND D AA+   ++  D +    +K+ V +GDAI  M+A+ A     +D++D+DP+G AA FLDA VQ V +GGLLCVT TDM VLSG  PE  +A+YGS+PT+  Y HEM+LRI+L A  A A R+RR + PVLS+++D YVR+FVRV  S    +  A  KA+YV QS  C SFFL PV                                                 M+  +  P     DG+C +TG R K GGPIWS  +HN  WV   +            +  +               R++ +L +VS ELPDVPLFY+L DM +TL     PR   +  AL +AG+R S  HKEP A+KTDAPD  VWDV+RCW + +P     + +  G AIL +      +ADF      V  G    +  +R    +  NPE  WGP +R +   R   E+ G
Sbjct:   58 VFYNKVQEFNRDLSIHVIKLFAEKRLQEKTEKALRKTRKREGAEFTAQDLEGLDATD-------WSQRAADSAST--------------------------------DGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVQAAQRNIDFNQVDVSKIRVQQGDAISAMHASSAAKQ-RFDVIDLDPYGSAAPFLDAAVQCVEEGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGKYLHEMALRIVLHALEAQANRYRRHVVPVLSLSIDFYVRLFVRVFTSA-AEVKKACTKAAYVHQSLGCGSFFLHPV-----------------------------------------------GKMSNNNYNPSLTTGDGLCPQTGKRFKVGGPIWSPAIHNMEWVRLLLDRVQKNRGPYQPSTKK---------------RIHGMLTSVSEELPDVPLFYSLPDMCSTLHCNS-PRMIDMQAALVNAGFRVSQQHKEPQAVKTDAPDDVVWDVMRCWVKKHPVSDKRKTSSPGQAILSKDPNH--QADFT-----VPSGLWQRKTATR----YQQNPEPYWGPKSRATGNKRGAEESCG 544          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: F0Y1C9_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y1C9_AURAN)

HSP 1 Score: 286 bits (732), Expect = 9.450e-83
Identity = 193/487 (39.63%), Postives = 256/487 (52.57%), Query Frame = 1

Query:  904 VLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGAGNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARA---IAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNP-------QPPPVRAAGLAILGRGTQLITRADFG---AGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLA 2325
            VLDA AA+G+R++RY  EVP VR+VVAND DA A A   + A  +GA +    + GDA+D M A+R  G   +D++D+DP+G  A FLDA VQAV DGGLLCVTSTDM VLSG  PEV +A+YGS+PT++ + HE +LRIL+ A  AAA +++R + PVLS+++D YVRVFVRV + R   +  +  K ++VLQS  CPSF+L P+                                   R  +  GG  A       + AP        C ETG   K GGPIWS PLH+  WV      +A   D   ST                     R++ +L AVS EL DVPL+Y L D+ + L     P+  ++  AL  AGYR S  H+EP A+KTDAP   +WDV RCW + +P       +P   R+A   IL   T  +  A+F    A R    + R               NPE++WGP A
Sbjct:    3 VLDALAATGLRSIRYAKEVPGVRKVVANDVDADAAAACGRNAAENGAADICEAHCGDAVDFMTASRGKGSQGFDVIDLDPYGSCAPFLDAAVQAVNDGGLLCVTSTDMTVLSGNYPEVCFAKYGSMPTKAKHLHEFALRILVHAIEAAAAKYQRHVVPVLSLSIDFYVRVFVRVYK-RPAEVKKSAAKRAFVLQSAGCPSFYLQPLA----------------------------------RSLDREGGHAAALLHRKATVAPA------FCGETGAPFKIGGPIWSAPLHDPEWVLAGLDRVAAGVDAHLSTT-------------------ERIHGMLTAVSEELLDVPLYYTLPDLCSALHCAS-PKMAEVRAALTHAGYRVSQHHREPDAIKTDAPPRVLWDVFRCWVKKHPVSEKRLAEP---RSAAAKILA--TAPVLEANFATTAAMRAKATKARR-----------WAPNPEEHWGPKA 412          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: W7T9F0_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7T9F0_9STRA)

HSP 1 Score: 281 bits (719), Expect = 8.120e-79
Identity = 216/611 (35.35%), Postives = 306/611 (50.08%), Query Frame = 1

Query:  571 VFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKR--SPETLSTQPSPVDGFEHRGAARRRRRWGLKAVLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGAG-NKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNPQPPPVR---AAGLAILGRGTQLITRADFGAGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLARRSVMAR--DEG--ETEGLE 2373
            VF+N+V  LNRDLS++M  + AE R +++   K+ KK   +PE ++ Q + VD             W  +     A                                 +GLVVLDA AASG+R++RY  EVP ++++V ND +  A+    +    +     ++   +G+A   MY   A     +D++DIDP+G AA F+DA VQAV +GGLLCVT TD+ VL+G  PEV W +Y ++PT++ Y HEM++RI+LQA    A R+RR I P+LSV +D Y+RVFVRV  S    +  A  K +YV QS  C SF+L             QTA                      + +  +G G   A+  P            VC ETG   + GGPIWS P+H+  WV  A+  A ++    +A+ +   G+A     L    R+  +L AVS EL DVPL Y L+DM  TL     PR + +  AL +AGYR S  HKEP A KTDAP A +WD++RCW + +P  P  +   +    IL +  +L  +A+F    +   E  A  +  SR    +  NPE  WGP ++     R  +EG  E EGLE
Sbjct:   82 VFYNKVQVLNRDLSIMMLRLFAERRLRDK-EAKRLKKEGLTPEQVTAQLADVD-------------WTARVAETAAE--------------------------------EGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQ-HAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTS-PAEVKKASLKMAYVHQSLGCGSFYL-------------QTAG---------------------KYSGDAGNGSYQASTGP--------TCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQA---DADAEAKTGLAP----LPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTC-PRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKL--QANF----SWPPELLAPKKKVSR----YPPNPEAFWGPKSKAKGRKRKMEEGGREKEGLE 584          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: A0A836C8Z8_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C8Z8_9STRA)

HSP 1 Score: 279 bits (713), Expect = 8.990e-79
Identity = 172/418 (41.15%), Postives = 233/418 (55.74%), Query Frame = 1

Query:  892 DGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGA-GNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNP 2142
            +G+V+LDA AA+ +R++RY  E+P VR+VVAND DAAA+   RK A+ +     KL V +GDA   MY  R+     +D++D+DP+G AA F+D  VQAV  GGLLCVTSTDM VL GA  E  +A+YGS+PT+  Y HEM+LRILL    ++A +++R + PVLS+++D YVR+FVRV  S   A  AA  +  YV+QS TC SFF+ P+                   C                   T+G     A M P +          VC +TGG +K GGP+W+ P+H+E WV   +A        T      PP  A          R+ S+L  VS EL DVPL YNL D+ + L     P       A+ +AGYR S  HK+P A+KTDAPD  +WD++RCW + +P
Sbjct:   45 NGIVILDALAATALRSIRYTKEIPGVRKVVANDLDAAAVEAARKNAEFNNVEAGKLDVLQGDATLLMYTRRSHEQ-QFDVIDLDPYGSAAPFVDGAVQAVKSGGLLCVTSTDMAVLGGANTEACFAKYGSMPTKGKYLHEMALRILLHLLDSSATKYKRHVVPVLSLSIDFYVRIFVRVFDSPSQAK-AATARRGYVMQSTTCSSFFVQPM-------------------C------------------RTAGNNLKPAVMIPEAA---------VCPQTGGHMKLGGPLWTAPIHDEQWVLALLARIERYKGGT------PPATA---------PRIKSMLTTVSEELFDVPLHYNLADICSHLHCTAPPL-PTFRAAVINAGYRISAQHKDPGAIKTDAPDDVIWDIMRCWVKTHP 398          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: A0A7S3ZTX3_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZTX3_9STRA)

HSP 1 Score: 272 bits (695), Expect = 1.730e-75
Identity = 192/535 (35.89%), Postives = 271/535 (50.65%), Query Frame = 1

Query:  547 GEQDGEGAVFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKRSPETLSTQPSPVDGFEHRGAARRRRRWGLKAVLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGAGNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARA---IAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNP 2142
            G+   +G VF+N+V   NRD+SVL+  + A+ R                        ++  E +  A  R+  G  A    A R   +                            GL VLDA AA+G+R++RY  EVP V++V++ND +A A    ++ A  +G  + +    GDA+  M  +R      YD++D+DP+G AA FLD  VQA++DGGLLCVTSTDM VLSG  PEV +A+YG++PT++ + HE +LR+LL A  A+A R+ R I PV+S+++D Y+RVFVRV +S          +A  VLQS  CPSF+L P+   T +R    T      R    P     +   +QR A                EA  P      CAETG   K GGPIWS P+H+  WVA A   +A   D+  ST                     R++ +  AVS EL DVPL+Y + D+  TL     P+  ++  AL+ AGYR+S  H++P A+KTDAP   +WDV RCW + +P
Sbjct:   64 GKGKDDGPVFYNKVQVFNRDVSVLVMALFAQWRY-----------------------IEKRERQAGADARKAGGDGAAAAEAERARLYALSAAELDELLREDAATG----------GLRVLDALAATGLRSVRYAKEVPGVKKVISNDIEAEAAKKCQENATSNGCESLVEAVCGDAVAHMINSRGD---PYDVIDLDPYGSAAPFLDGAVQAISDGGLLCVTSTDMTVLSGGYPEVCYAKYGAMPTKAKHLHEYALRVLLHAIEASATRYARHIVPVVSLSIDFYIRVFVRVYKSPSNVKHTIGRRA-LVLQSAGCPSFYLQPLGSPT-QRDGGYTCG----RLHEPPRKINQMGRRAQRRAEXXXXXXXXXXXDFDPEAVAPAR----CAETGAAFKIGGPIWSAPIHDAEWVAAALDRVAAECDEHLSTT-------------------ERIHGVFTAVSEELMDVPLYYTIPDICKTLHCAA-PKLCEVRAALQHAGYRSSQSHRDPDAIKTDAPPRVLWDVFRCWVKKHP 532          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: K3WLD8_GLOUD (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WLD8_GLOUD)

HSP 1 Score: 259 bits (662), Expect = 1.210e-71
Identity = 192/611 (31.42%), Postives = 286/611 (46.81%), Query Frame = 1

Query:  571 VFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKRSPETLSTQPSPVDGFEHRGAARRRRRWGLKAVLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSGAG-NKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNP---QPPPVRAAGLAILGRGTQLITRADFGAGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLAR----RSVMARDEGETEGLESP 2379
            VF+N V  LNRDLS+ + +  A  RA E+L   K+ K++     T+ +P +  E            + A L                                     G+ + +A +ASG+R++RY  ++P V+ ++ ND D AA+  I++  + +    ++++ N  DA D MY  R      +D++D+DP+G A+ FLD  VQ+V +GGLLCVT TDM VLSG  PEV ++RY S+P +S Y HEM+LRI+LQ+  A A ++ R I P+ + ++D YVRVFVRV +S    + AA  K SY  Q   C SF L                               PL V S    + S G               P+V    C +  G+ K  GPIWS P+H+   V R     +   T     +                 R++ +L  +S EL D PL Y L  +  TL    +PR EQI GA+ +AGY+AS  HK P A+KT+AP+  +WD++RCW + +P   +       G+ IL +  +    A+F + R   ++ +A             HNPE NWGP AR    ++      G  +G + P
Sbjct:   40 VFYNNVQVLNRDLSIAVISDFARVRAVEKL--HKQAKKTAAAAGTEFTPKEYTEEE----------IDAHLAENAE------------------------------SSGIKIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVESIKRNIEFNELPLDRVIPNEDDATDVMYRHRKD-KDHFDVIDLDPYGSASIFLDGAVQSVANGGLLCVTCTDMAVLSGKNPEVCFSRYNSIPNKSNYLHEMALRIVLQSIEATANKYARHIVPIAACSIDFYVRVFVRVYKSPVN-VKAAMTKLSYTFQCVGCDSFHL------------------------------QPLGVASGNTFHASRG---------------PVVGQ-ECDQCSGKFKMAGPIWSAPIHDRDVVLRIRERVAKTTTEFPTKE-----------------RLHGLLTTISEELVDAPLHYTLPGLSKTLHCS-NPRMEQIQGAIRAAGYQASQFHKVPEAIKTNAPNDVIWDIMRCWVKKHPVTKKREGENTPGMRILAKEPKF--EANFSSRRPAHEKEKA---------LRFPHNPEPNWGPKARAVGNKAAAPAQNGSAQGEQPP 531          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: A0A067CL21_SAPPC (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067CL21_SAPPC)

HSP 1 Score: 255 bits (652), Expect = 2.050e-70
Identity = 197/612 (32.19%), Postives = 285/612 (46.57%), Query Frame = 1

Query:  571 VFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKRSPETLSTQPSPVDGFEHRGAARRRRRWGLKA-VLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSG-AGNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNPQPPPVRAAGLAILGRGTQLITRADFGAGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLARRSVMARDE------GETEGLESPL 2382
            VF+N+V  LNRDLS+ + N  A ERA+E +  K+RK+   E L T                     +KA VL  A                                  GL + +A +ASG+R++RY  E+  V+ ++ ND D AA+  I++  + +G   +KL+ N  DA   MY  R      +D++D+DP+G A+ FLD  VQA+ +GGLLCVT TDMPVLSG  P+V + RYGSVP +S Y HE +LR++L +  +AA R+++ I P++S ++D YVRVFVRV +S    + A+  K SYV Q   C SF + P+     K       E         PE                                       +C + G + K  GPIWS PLH+   V +       +G +     DR                ++ +L +VS E+ D PLFY L  +  TL   Q PR +Q+  A+ +AGY+ S  HK P A+KTDAP+  +WD++RCW + +P    +     +    GTQ++ +         V  G+    AG         NPE  WGP +R   +A  E      GE EG +  L
Sbjct:   33 VFYNKVQVLNRDLSIAVINQFAHERAREAIL-KQRKREKNEDLPTDDE------------------VKAHVLAHAET-------------------------------SGLKIFEALSASGLRSIRYWQEIAGVKSILVNDLDPAAVISIKRNIEFNGLTTDKLIPNEDDATSVMYRHRKEA-DNFDVIDLDPYGSASIFLDGAVQAIANGGLLCVTCTDMPVLSGKDPDVCFHRYGSVPYKSNYLHENALRMVLHSIESAAIRYQKHIVPIVSCSIDFYVRVFVRVYKSPVN-VKASMTKLSYVYQCTGCDSFHMQPLGKFNGKTYHASFVE---------PERA-------------------------------------LCDQCGKKFKMSGPIWSNPLHDRDLVLKIRENVIKEGVAKYPTKDR----------------LHGLLTSVSEEVVDAPLFYTLHGICKTLHCGQ-PRMDQMQSAILNAGYKVSQSHKVPEAIKTDAPNDVIWDIMRCWVKKHP----ISKKHESEKDAGTQILAKEPK---HEAVFTGKRP-SAGKEKALRFPKNPEAYWGPKSRARGLAGAELARVVEGEPEGKKPKL 521          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: A0A383WLZ9_TETOB (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WLZ9_TETOB)

HSP 1 Score: 256 bits (655), Expect = 9.570e-70
Identity = 202/597 (33.84%), Postives = 273/597 (45.73%), Query Frame = 1

Query:  571 VFFNRVGALNRDLSVLMANVLAEERAKERLPGKKRKKRSPETLSTQPSPVDGFEHRGAARRRRRWGLKAVLKRATRRHSWRXXXXXXXXXXXXXXXXXXXXXXXXXGDGLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSG--AGNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIAMASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNP-QPPPVRAAGLAILGRGTQLITRADFGAGRTGVDEGRAGGEAGSRGGFLHTHNPEQNWGPLARRSVMARDE 2352
            VF+N     NRDLSV +      + A+E+  GK RK                    G A++ +R   KA  K A                                  G  VL+  AASG+R++RY  E+  V R+ AND D A +  +R    L+G     ++V    DA   M          YD +D+DP+G  A FLD+ +QAV +GGLL VT+TDM VL G   EV W +YGS P    Y HE ++RILL    + A R++R I P+LSV +D Y+RVFVRV  S    + A+  K +YV QS+ C S++L                                 PVG Q++  +S              A  P V    C E+G     GGPIW+ PLH++AWVA  +    ++                   R    AR+  IL +V  ELPDVPL+YN+ DM  T+     PR EQ+  AL +AG+R SG H  PLALKTDAP   VWD++RCW + +P +PP   +   AIL +  ++ T ADF      V E     +AG +       NP   WGP AR     + E
Sbjct:   34 VFYNEAQVTNRDLSVAVLRRFLPQLAQEKAEGKLRKP-------------------GQAQKDKRQAKKAAAKAAA-----------------------ADEPVQPAQTGARVLEGLAASGLRSIRYALELDGVSRIDANDLDPAVVESMRANIALNGPRVAARVVPTCSDARLLMLQNPE----AYDAIDLDPYGTPAHFLDSALQAVAEGGLLAVTATDMAVLCGNSGEVCWTKYGSYPIHRPYCHEAAVRILLACLESHANRYKRYIVPLLSVHMDFYIRVFVRVFTSA-AEIKASASKQAYVWQSQGCDSWWL--------------------------------QPVGQQQQKGSS---------IKHMPAHGPAVPSNRCPESGAAFLMGGPIWARPLHDQAWVAGLLQQMEEESG-----------------RYAQHARLKGILTSVLEELPDVPLYYNVHDMCRTVRCTP-PRAEQLRSALINAGFRVSGSHANPLALKTDAPPKVVWDIIRCWVKLHPIKPPEPNSYPAAILSK--EIETEADFRKAAGAVPESV---KAGVK---RFVQNP-AFWGPKARHGRPLKPE 515          

BLAST of mRNA_P-fluviatile_contig18.3256.1 vs. uniprot
Match: A0A7S0UWQ1_9CHLO (tRNA (guanine(26)-N(2))-dimethyltransferase (Fragment) n=1 Tax=Polytomella parva TaxID=51329 RepID=A0A7S0UWQ1_9CHLO)

HSP 1 Score: 254 bits (649), Expect = 1.100e-69
Identity = 168/447 (37.58%), Postives = 233/447 (52.13%), Query Frame = 1

Query:  895 GLVVLDAFAASGVRALRYLTEVPTVRRVVANDYDAAAIAMIRKAADLSG--AGNKLVVNRGDAIDTMYAARAGGLGTYDLVDIDPFGCAAGFLDAGVQAVTDGGLLCVTSTDMPVLSGAQPEVAWARYGSVPTRSGYHHEMSLRILLQATSAAAGRHRRSIEPVLSVAVDHYVRVFVRVSRSRKGALVAAQEKASYVLQSETCPSFFLLPVLPTTPKRSPRQTAESRPRRCPGNPESTAPLPVGSQREANTSGGGGADAAMTPGSEAPRPLVFDGVCAETGGRVKAGGPIWSGPLHNEAWVARAIA-MASDKGTSTNANDDRPPGVAVPRPRLVAGARVNSILRAVSRELPDVPLFYNLRDMFATLGFKQHPRREQIMGALESAGYRASGMHKEPLALKTDAPDAAVWDVLRCWARDNPQPPPVR---AAGLAILGRGTQLITRADF 2217
            G  VL+  AASG+R++RY  EVP++ R+ AND D AA A + +  +L+G  A +K+     DA   M +       TYD VD+DP+G  A  LD+ VQ+VT+GGLL VT+TDM  L G         YGS P    Y HEM+LRILL    A A R++R I P+LS+++D YVRVFVRV  S      +A  K +Y+ QS  C SF++                                 PVG QR+ N+S          PG   P P +    C +TG     GGPIWS P+H + +V   +A    DKG                     A  ++  +L +   ELPDVPL+Y+L D+  T+   Q P+++    AL +AG+R SG H +PLA+KTDAP AA+WD+LRCW +D+P P  V+   +    +L +  Q++  ADF
Sbjct:  115 GARVLEGLAASGLRSIRYALEVPSIGRIDANDLDHAASAAMLRNIELNGGAAKDKIFAQNLDARILMMSNPI----TYDAVDLDPYGTPAHLLDSAVQSVTEGGLLLVTATDMANLCGNNGTACHNNYGSYPVHRPYCHEMALRILLGCIEAHANRYKRHIVPLLSLSIDFYVRVFVRVYTSPGACKESATTKQAYIWQSSGCDSFWM--------------------------------QPVGQQRKQNSS------CKFLPG---PGPAMEAARCPDTGSGFIMGGPIWSAPIHQQDFVRGLLADFEEDKGQC-----------------FAAYPKIRGLLTSALEELPDVPLYYDLHDLCKTVHC-QPPKKDVFRAALLNAGFRVSGSHAKPLAIKTDAPPAAIWDILRCWIQDHPAPAAVKDPNSYSAKLLAKAPQIV--ADF 496