prot_P-fluviatile_contig8.14436.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: D7G2M8_ECTSI (E3 ubiquitin-protein ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2M8_ECTSI)

HSP 1 Score: 1456 bits (3770), Expect = 0.000e+0
Identity = 1150/2330 (49.36%), Postives = 1317/2330 (56.52%), Query Frame = 0

Query:   15 DGASAAIDLTYLALRDACQQ-------KXXXXXXXXXXXXXXXXXXTRPSETRLFGNILRAVLLREEGEEREDEQNKEHLVHVIPGAFNVLTRLLAADEALAGCYAPALPAAASSRLADVSAAGDEAEASVTAAVAVXXXXXXXX-----TTKQGFLSITADDVDLKQPSTALSGGGG-------IVSISTLADLQQKLLPPDLPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLHELPPGLITGGTKVMGAVQTFLLEATYALVRSFDLSQPWTSDRGRTPKALSVMIHHTVYRETSLSEALASVAKIDVAKIHEVRWPLPSCDSPNTSRRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKADEAEVICFREGERGEEADRRELQRMLDIARGLSERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCDLFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSVSGGAPGNQDGKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFLRTCFTEWIKILVMVQQMAPQVRQQRAHVEREDRDWMFAFNINISLTSLFECMLGWLREPVRAAPIPAGFLSWEKPAAAAXXXXXAITHNRTSTSPSHADSEINGRRGGVXXXXXXXXXXXXXXXXXXGARPSAPPAGLIGQPSFVTFPPXXXXXXXXXXXXXSPSLSPTQAELFAAEG--GMPPSPWTAAGANAAMAAFLSEARRHVEGSAAPAMAAAAAATSQASTSIHPASEAVGAARSGRGGAEV-------------VAWEHQALPAYPAGVLWVRPPVAGRSFHIILHRFFAAMVREACHCEERERDLSALSSYVTCYPEVATDVADTALNVLSFAAEVRAGLWRKNGQCMNDQVVNYSEPSFCKTFRDLDLLTVQFAGMSCGATQLVNHILHKFQCFY-WIFDEYNLQATAGSYGNEKSASSVTSQPSSAAGGLGRSTSSSXATSDLAGAGVGEQGTSGQGSPAAGSTLAAATGTSLVLPTHIFSDPSASHTGMGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMPVVPDPLIYPAPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDSPEAEGGKSGRGGMNCRLARQLRRELVHRLASAPCTHSELQDTCHDMSQNEPLEAEVIERILRDVATRREPSSALEAGRFVLKPEVFAAEYDSTFFHQSLQAHQQASERRPPIKGPTPVAPPPPEAHPLFRQFRVDLLKSEATVRFVGQVLLSCTRKFSGRSRDTLLLRGLHLLTLAVHVMNSESAQASGGGGGLLQR---------------------DVEVFCGAISDPIVQPPTPPLHTGGTGAAGGGVRGNGTAVVRVADT---------REVSDDNA-NLRRLWGELDDDIAAEVATATSHVRSSVPRQVSPAAPSTARAAAPVSVVQLLLELEAAPWDHGDKLISCGVRWLLDRIGGMSKRCREIISPAPVPSSTPAAIESSKPSSVGWVVDGSTRGGDGGSSDLGNNGGASPGAGVGVSDGGS-----------------GDAAAAMEERKKAARKRAMDMIQQKASAFARQMDMLGSSSXXSVSSSSDGEVGNDGMGVVGGSXXXXXXXXXXXXXXXRDGLCSXXXXXXXTPDLGAGRFEAASEASGGAAXXXXXXXVGKRXXXXXXXXXXXXXXXXXXRQHARLQCIVCRDSDDRGALGFVGFGRRSRVLDIRPEGDFRCPGVHLQFCGHALHYTCFDRYYATMLHISDSHNNMVLDVEKGEFHCPLCKAVGNLMVPCFVP---ADEDXXXXXXXXXXXXXXGALRVVGEGSMEGQAQSIPGRRGSGLGGSGSLVSGEPKLSGRKGARRDAHTRTGTKGEEEGRGSEPSAAVAAAAAVARDGDAMDVSDDERNPTNAAAGSGIDGGVGGCWLDTKAALSLEMAKSSAFATRRGAXXXXXXXXXXHERT-----NRTGSRSGLLAMDGATDEGRGXXXXXXXXXXXXXXXXXGQFHGLNVETSSMRPQQQQQQATKRFLQSVYEVENDVTTVPPSVSTEELFLCACRALAYSVSSAAAATSPLESLRGHVAAAASTASGXXXXXXXXXXXXXXXXARVGAGGAAAWGGLDHADATRLRCLGLVAEALRKAAEQVEGLEETVRRKLAASLRG 2253
            D +S ++DLT+LALRDA +Q        XXXXXXXXXXXX       RPSET LFG+ILRAVLL+++  E   EQ++  LVH  PG F VLTRLLAADE    C  PAL A A+ R A    AG +        + +             T K   ++ +    D  Q      GGGG       +VSIS+L+D+QQKLLP D PRRVCQYAFLKNDIVWICK CQADETCVLCNDCFRSSDH+GHEVYFYHAQ  AGGCCDCGDPDAWD RGFC HHGLDNRDPL ELP GLIT G+ VM AVQTFLLE + A+V+SFDL++PW +  GR PK++S+++H ++Y E  + EAL SVA + +A +H+                                                                      G AD          ER  EA  REL+R+LD+AR L++RGLLISVRT E+  R+ R+ DV  WLHAVS LSDGCCRLVC++ DV TLVALMEAD  LPKPLACALHDLYLALMADQPFKTRIAAAYVR LP+VT +YARGVGTAE ALYTLSVQFLNR+AFVK LVE HHLLQILAGCL+STL+ AC RQ   GGAPGN+DGK+VL ASHSV+ HRRYNPI ADLKCVLNIEGVPQ FLR CFTEWIK+L+MVQ+MAPQVRQQR HVERE R+WMFAFNINISLTSLFECMLGWLREP     IPAGF S  K     XXXXX               S ++      XXXXXXXXXXXXX         S  P         V++              X        +E    +G  G P  PW  +GANA M  FL+EA   V            A                 +  S RG A +              AWEH+ LP +P GVL VRPP AGRSFHIILHRFFAAMVREAC+CEER  DL  L  +V  YPEV TDVADTALNVLSFAAEVRAGLWRKNGQCMNDQV+NY+EPSFCK FRDLDL+TVQFA +S GA+QLVNH+LH+FQCF+ WI DE +                                                                                          XXXXXXXXXXXXXXX                         EMP+VPDPL  P P                                        SPEA    +  GG   R+ARQLRRELVHRLASAPCTHSELQDTC+  S NE LEAE++E ILRDVATRREPS+AL++GRFVLKPEVFAAEYDSTFFHQS+QAHQQASERRP IKGPTPVAPPPPEAHPLF+Q RVDL+K E  +R VGQVLL C+RK  GRSRDTLLLRGLHLLTLAVHVM+  +A +                               D+E FC A+  P+VQP                                        E++D  A   R LWGELD          + +  S      S  A     A  P+SV+QLL+EL+AAPWD  DK +S  V+WLL+R+ GMS+RCREII+P P P++ P                                   SPG G+G   GG+                  +A A MEER+KAARKRAMDMI+QKASAFARQ+DM+                                 XXXXXXXXX      XXXXXXX            + AS    XXXXXXX    XXXXXXXXXXXXXXXXXX +  R +CIVCRD  DRG LGFVGFGRRS+VLD+RP+G+ R PGVHLQFCGHALHY+CF++YY TM+ ISDS+NN+ LDV++GEFHCP CKAVGNLMVPCF     A++   XXXXXXXXXXXX     VG G  E        R G G                                          AAV  A      GD MDVS++E + ++ A+G G D  VG  WLD KAALSLE A+ S FA R+  XXXXXXXXXX         +RTGS    +           XXXXXXXXXXXXXXXXX        +            AT++FL+SVYEVE  VT V PSV+ E LFL ACRALAYSVSSAA   SP++SLRG+ AA                XXXXXXX   G   AA    +DH  ATRLRCL LVA  LR+AA Q  GLEETVRRKLA SL G
Sbjct:   14 DASSVSVDLTFLALRDASRQGGXXXXXXXXXXXXXXXXXXPASTSCCRPSETPLFGSILRAVLLQQQAGEGGGEQDQS-LVHTTPGVFPVLTRLLAADEV---CQ-PAL-AGAADRAATAGGAGQQQRQPGHHQLRLQQLESAHQPRSSPTPKDALVASSRARHDDTQ-----GGGGGDVSHSNSLVSISSLSDVQQKLLPADTPRRVCQYAFLKNDIVWICKECQADETCVLCNDCFRSSDHEGHEVYFYHAQ--AGGCCDCGDPDAWDYRGFCRHHGLDNRDPLLELPQGLITAGSTVMAAVQTFLLETSKAVVQSFDLAKPWNTSGGRPPKSVSMIVHQSLYEENQVYEALGSVANLGMANVHQ--------------------------------------------------------------------GDGAADP---------EREREA--RELERLLDVARALAKRGLLISVRTHELQERLKRSQDVVRWLHAVSTLSDGCCRLVCEMLDVPTLVALMEADTHLPKPLACALHDLYLALMADQPFKTRIAAAYVRALPAVTANYARGVGTAEHALYTLSVQFLNRSAFVKKLVEKHHLLQILAGCLLSTLSRACQRQPARGGAPGNRDGKMVLSASHSVLEHRRYNPIAADLKCVLNIEGVPQRFLRACFTEWIKVLLMVQKMAPQVRQQRLHVEREQREWMFAFNINISLTSLFECMLGWLREPFGHTSIPAGFFS-SKTTXXXXXXXXXXXXXXXXXXXXXXPSPVSSGEXXXXXXXXXXXXXXXXAPLALFQLASPTPXXXXXXGEVVSWWADILEEEHVFAAAXXXXXXXXASEGEEKDGAEGEPWQPWAGSGANATMWEFLNEANNAVP-----------AXXXXXXXXXXXXXXXXXSGNSHRGAASLWSALATATVDGTRAAWEHEELPTFPGGVLRVRPPAAGRSFHIILHRFFAAMVREACNCEERGSDLRQLCGFVGRYPEVVTDVADTALNVLSFAAEVRAGLWRKNGQCMNDQVLNYAEPSFCKIFRDLDLMTVQFAALSGGASQLVNHVLHRFQCFFFWILDECDATTXXXXXXXXXXXXXXXXXX---------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSTLDPDQQLALGEECLMLLILLVTEMPMVPDPLT-PEPGVDSGDGGGTGVGGRGGG----------------------SPEAARKGTAGGG---RMARQLRRELVHRLASAPCTHSELQDTCYASSHNEALEAEMMEAILRDVATRREPSNALDSGRFVLKPEVFAAEYDSTFFHQSIQAHQQASERRPAIKGPTPVAPPPPEAHPLFKQLRVDLVKDETMLRVVGQVLLDCSRKTPGRSRDTLLLRGLHLLTLAVHVMDPSTASSGXXXXXXXXXXXXXXXXXXXXXXXXXXXQGSDLEAFCSAMVQPMVQPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEEIADVPAPRTRTLWGELD----------SVYTHSPPXXXXSLWARHRGEAPPPLSVLQLLVELDAAPWDQSDKFLSSSVKWLLERLAGMSERCREIITPTPSPAACP-----------------------------------SPG-GIGGKSGGAVEKXXXXXXXXXXXXXXXNATAVMEERRKAARKRAMDMIEQKASAFARQIDMM---------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKEQRPECIVCRDDADRGPLGFVGFGRRSQVLDVRPDGEERSPGVHLQFCGHALHYSCFEKYYVTMVQISDSNNNVALDVDQGEFHCPFCKAVGNLMVPCFEESSEAEDSDWXXXXXXXXXXXXXXXXXVGVGGKE--------RLGKG------------------------------------------AAVGHAG-----GDPMDVSEEE-DQSSGASGDGDDD-VG--WLDRKAALSLETARPSMFAARQSXXXXXXXXXXXXXXXXXXXXDRTGSGFVYMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQLFDS--------ATRKFLESVYEVETGVTPVLPSVTPEALFLSACRALAYSVSSAATTASPIDSLRGYAAA----------------XXXXXXXXXEGXXAAA----IDHGGATRLRCLRLVAGVLRRAAGQA-GLEETVRRKLAGSLAG 2031          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A6H5JTE4_9PHAE (E3 ubiquitin-protein ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTE4_9PHAE)

HSP 1 Score: 1170 bits (3028), Expect = 0.000e+0
Identity = 788/1576 (50.00%), Postives = 919/1576 (58.31%), Query Frame = 0

Query:   15 DGASAAIDLTYLALRDACQQKXXXXXXXXXXXXXXXXXXT-------RPSETRLFGNILRAVLLREEGEEREDEQNKEHLVHVIPGAFNVLTRLLAADEALAGCYAPALPAAASSRLADVSAAGDEAEASVTAAVAVXXXXXXXX-----TTKQGFLSITA---DDVDLKQPSTALSGGGGIVSISTLADLQQKLLPPDLPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLHELPPGLITGGTKVMGAVQTFLLEATYALVRSFDLSQPWTSDRGRTPKALSVMIHHTVYRETSLSEALASVAKIDVAKIHEVRWPLPSCDSPNTS----RRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKADEAEVICFREGERGEEADRRELQRMLDIARGLSERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCDLFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSVSGGAPGNQD---GKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFLRTCFTEWIKILVMVQQMAPQVRQQRAHVEREDRDWMFAFNINISLTSLFECMLGWLREPVRAAPIPAGFLSWEKP-----------------------------------------AAAAXXXXXAITHNRTS-------------------------------TSPSHADSEINGRRGGVXXXXXXXXXXXXXXXXXX-------GARPS----APPAGLIGQPSFVTFPPXXXXXXXXXXXXXSPSLSPTQAELFAAEGGMPPSPWTAAGANAAMAAFLSEARRHVEGSAAPAMAAAAAATSQASTSIHPASEAVGAARSGRGGAEVVAWEHQALPAYPAGVLWVRPPVAGRSFHIILHRFFAAMVREACHCEERERDLSALSSYVTCYPEVATDVADTALNVLSFAAEVRAGLWRKNGQCMNDQVVNYSEPSFCKTFRDLDLLTVQFAGMSCGATQLVNHILHKFQCFY-WIFDEYNLQATAGSYGNEKSASSVTSQPSSAAGGLGRSTSSSXATSDLAGAGVGEQGTSGQGSPAAGSTLAAATGTSLVLPTHIFSDPSASHTGMGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMPVVPDPLIYPAPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDSPEAEGGKSGRGGMNCRLARQLRRELVHRLASAPCTHSELQDTCHDMSQNEPLEAEVIERILRDVATRREPSSALEAGRFVLKPEVFAAEYDSTFFHQSLQAHQQASERRPPIKGPTPVAPPPPEAHPLFRQFRVDLLKSEATVRFVGQVLLSCTRKFSGRSRDTLLLRGLHLLTLAVHVMNSESAQASGGGGGLLQR--------------------DVEVFCGAISDPIVQP 1464
            D +S ++DLT+LALRDA +Q                           RPSET LFG+ILRAVLL+++  E   EQ ++ LVH+ PG F VLTRLLAADE    C  PAL A A+ R A    AG +        + +             T K   ++ +    DD+        +S    ++SIS+L+D+QQKLLP D PRRVCQYAFLKNDIVWICK CQADETCVLCNDCFRSSDH+GHEVYFYHAQ  AGGCCDCGDPDAWD RGFC HHGLDN DPL ELP GLIT G+ VM AVQTFLLE + A+++SFDL++PW +  GR PK++S+++H ++Y E  + EAL SVA + +A +H++R+ L +  +          F  G   A +              + +   D         A A+ +    +++ G+ D A        +   E D REL+R+LD+AR L +RGLLISVRT E+  R+ R+ DV  WLHAVS LSDGCCRLVC++ DV TLVALMEAD  LPKPLACALH+LYLALMADQPFKTRIAAAYVR LP+VT +YARGVGTAE ALYTLSVQFLNR+AFVK+LVE HHLLQILAGCL+STL+ AC RQ   GGAPGN+    GK+VL ASHSV+ HRRYNPI ADLKCVLNIEGVPQ FLR CFTEWIK+L+MVQ+MAPQVRQQR HVERE R+WMFAFNINISLTSLFECMLGWLREP++   IPAGF S +                                           AA A      +T                                    TSP+ A + +     G              XXXXX       G RPS    A P                    XXX XXX         E   AE   P  PW+ +GANA M  FL+EA                     +  S   A+    A  +        AWEH+ LP +P GVL VRPP AGRSFHIILHRFFAAMVREAC+CEER  DL  L  +V  YPEV TDVADTAL+VLSFAAEVRAGLWRKNGQ MNDQV+NY+EPSFCK FRDLDL  VQFA +SCGA+QLVNH+LH+FQCF+ W+ +E +   TA +    +SA                     X                                                      XXXXXXXXXXXXXXX                         EMP+VPDPL  P P       XXXXXXXXXXXX                      PEAEG     GG   R+ARQLRRELVHRLASAPCTHSELQDTC+  S NE LEAE++E ILRDVATRREPS+AL++GRFVLKPEVFAAEYDSTFFHQSLQAHQQASERRP IKGPTPVAPPPPEAHPLF+Q RVDL+K E  +R VGQVLL C+RK  GRSRDTLLLRGLHLLTLAVHVM+  +A + G  G                         D+E FC AI  P+VQP
Sbjct:   14 DTSSVSVDLTFLALRDASRQGGNSSSSGPSATATATTVPQPASTSCYRPSETPLFGSILRAVLLQQQAGEGGGEQ-QQSLVHMTPGVFPVLTRLLAADEV---CQ-PAL-AVAADRAATAEGAGRQQRQPGDHQLRLQQLESAHQSRSSPTPKDPLVASSRAQQDDIQXXXXXXDVSHSNNLISISSLSDVQQKLLPADTPRRVCQYAFLKNDIVWICKDCQADETCVLCNDCFRSSDHEGHEVYFYHAQ--AGGCCDCGDPDAWDYRGFCPHHGLDNGDPLQELPQGLITAGSAVMAAVQTFLLETSKAVMQSFDLTKPWNTSGGRPPKSVSMIVHQSLYEENQVFEALGSVANLGMANVHQIRYILATGSAEEAEIMSFPSFPFGAAAAGTXXXXXXXXXXXXXXVVAPTGDDG-------AAASVAGDTAVVSGGEGDGA-------ADPERERDARELERLLDVARALVKRGLLISVRTHELQERLKRSQDVVRWLHAVSTLSDGCCRLVCEMLDVPTLVALMEADTHLPKPLACALHELYLALMADQPFKTRIAAAYVRALPAVTANYARGVGTAEHALYTLSVQFLNRSAFVKELVEKHHLLQILAGCLLSTLSRACKRQPARGGAPGNRGFIAGKMVLSASHSVLEHRRYNPIAADLKCVLNIEGVPQRFLRACFTEWIKVLLMVQKMAPQVRQQRLHVEREQREWMFAFNINISLTSLFECMLGWLREPLKDTSIPAGFFSSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTAAPAPLALFQVTFGALVASYEASRERXXXXXXXXXXXXXXXXXXSPQLTSPAAAAAAVEEVASGWESVGAAGSGAVEAXXXXXXXXXXXXGGRPSPDQLASPXXXXXXXGREVVSWWADIMEXXXIXXXXXXXXSEGEEKDGAEE-EPWQPWSGSGANATMWEFLNEANNAXXXXXXXXXXXXXXXXXXSGNSHRGAASLWSALATATVDGRRAAWEHEELPTFPGGVLRVRPPAAGRSFHIILHRFFAAMVREACNCEERGSDLRQLCGFVGGYPEVVTDVADTALDVLSFAAEVRAGLWRKNGQSMNDQVLNYAEPSFCKIFRDLDLTAVQFAALSCGASQLVNHVLHRFQCFFFWMLNECDATTTAQTSAAGESAXXXXXXX------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSTLDPDQQLALGEECLMLLILLVTEMPMVPDPLT-PEPGVDSGDGXXXXXXXXXXXX----------------------PEAEGKGMAGGG---RMARQLRRELVHRLASAPCTHSELQDTCYASSPNETLEAEMMEAILRDVATRREPSNALDSGRFVLKPEVFAAEYDSTFFHQSLQAHQQASERRPAIKGPTPVAPPPPEAHPLFKQLRVDLVKDETMLRVVGQVLLDCSRKTPGRSRDTLLLRGLHLLTLAVHVMDPSTASSGGPSGAGRDAAXXXXXXXXXXXXXXXXHGSDLEAFCSAILQPMVQP 1528          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: W7U0V5_9STRA (E3 ubiquitin-protein ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U0V5_9STRA)

HSP 1 Score: 431 bits (1107), Expect = 1.000e-117
Identity = 505/1903 (26.54%), Postives = 672/1903 (35.31%), Query Frame = 0

Query:  200 PRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLHELPPGLITGGTKVMGAVQTFLLEATYALVRSFDLSQP----WTSDRGRTPKALSVMIHHTVYRETSLSEALASVAKIDVAKIHEVRWPLPSCDSPNTSRRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKADEAEVICFREGERGEEADRRELQRMLDIARGLSERGLLISVRTDEVTRRMDRALDVA----EWLHAVSALSDGCCRLVCDLFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSV--------------------------------------SGGAPGNQDGKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFLRTCFTEWI-KILVMVQQMAPQVRQQRAHVEREDRDWMFAFNINISLTSLFECMLGWLREPVRAAPIPAGFLSWEKPAAAAXXXXXAITHNRTSTSPSHADSEINGRRGGVXXXXXXXXXXXXXXXXXXGARPSAPPAGLIGQPSFVTFPPXXXXXXXXXXXXXSPSLSPTQAELFAAEG-GMPPSPWTAAGANAAMAAFLSEARRHV----------------------------------------EGSAAPAMAAAAAATSQASTSIHPASEAVGAARSGRGGAEVVAWEHQALPAYPAGVLWVRPPVAGRSFHIILHRFFAAMVREACH--------CEERERDLSALSSYVTCYPEVATDVADTALNVLSFAAEVRAGLWRKNGQCMNDQVVNYSEPSFCKTFRDLDLLTVQFAGMSCGATQLV-NHILHKFQCFYWI----FDEYNLQATAGSYGNEKSASSVTSQPSSAAGGLGRSTSSSXATSDLAGAGVGEQGTSGQGSPAAGSTLAAATGTSLVLPTHIFSDPSASHTGMGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMPVVPDPLIYPAPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDSPEAEGGKSGRGGMNCRLARQLRRELVHRLASAPCTHSELQDTCHDMSQ-NEPLEAEVIERILRDVATRREPSSALEAGRFVLKPEVFAAEYDSTFFHQSLQAHQQASERRPPIK---GPTPVA--PPPPEAHPLFRQFRVDLLKSEATVRFVGQVLLSCTRKFSGRS-----------------------------------------RDTLLLRGLHLLTLAVHVMNSESAQASGGGGGLLQRDVEVFCGAISDPIVQPPTPPLHTGGTGAAGGGVRGNGTAVVRVADTREVSDDNANLRRLWGELDDDIAAEVATATSHVRSSVPRQVSPAAPSTARAAAPVSVVQLLLELEAAPWDHGDKLISCGVRWLLDRIGGMSKRCREIISPAPVPSSTPAAIESSKPSSVGWVVDGSTRGGDGGSSDLGNNGGASPGAGVGVSDGGSGDAAAAMEERKKAARKRAMDMIQQKASAFARQMDMLGSSSXXSVSSSSDGEVGNDGMGVVGGSXXXXXXXXXXXXXXXRDGLCSXXXXXXXTPDLGAGRFEAASEASGGAAXXXXXXXVGKRXXXXXXXXXXXXXXXXXXRQHARLQCIVCRDS---DDRGALGFVGFGRRSRVLDIR-PEGDF-RCP-----------------------------------------------------GVHLQFCGHALHYTCFDRYYATMLHISDSHNNMVLDVEKGEFHCPLCKAVGNLMVPCFVPADED 1896
            P R+CQ+AF +NDIVWICK CQ DETCVLCNDCFR S H+GHEVYFY  Q  AGGCCDCGD DAWD  GFC  HG D  DPL  LPP  I G    M     FL +A  AL  S+DL       W  +            + T++ +             D+    EV   L  C   N      L  V    GE                                    R    S                        L+ +   AR L  R L +S+    VTRR+ +   VA    +WL+ ++ +S G CRLV +    +TL  LM A   LPKPLA ALH LYL LMADQ FK  +A +Y     +VT  YA G+G AE ALYTLSVQFLNR+AFV DLV   H L++L   L   +  A G  S                                       S G  GN      L  SH +  HRRY+P+VADLK VLNI GV  HF  TC   W+ ++L+ +Q +  Q R+   HV  E R+WM+AFN+ ISL  + +C+L W    VR AP+          A AA      I           A  E    RG                    G++ S   AG+                          S   T  + F ++G G+    W  A                                                          G             S AS+S+ P +  +     G      V++     P +P G L +      RSFH+ILHR FA +VREA              E DL  L ++++  P +A  + +  ++VL  +AE+RAGLWR+NGQ M DQV+NY EP FC+ FRD+DLL VQ A ++ G  + V N +L +F  + W+    +++      AG  G ++ A                                                                +D S+     GG                                        E+P+ P P                                               P+             R    LRRE+VHRLAS PCTHSE+ + CH  S  +E +   +IE IL  VA RRE    LEA RFVL    +  EYD  FFH + QAHQQASE RP  K   GP+P A   PPP AHP+F   R+ LL S   ++ +  VL+      SGRS                                         RD+L+ R LHLLTLAVH +                RD               P+ P                                               A     A  H  S++P   SP            S+  LLL L ++     D  +  G +WLL  +G +   C+             AA++ S                                  V V +G    A +A   RKK AR++ +  +Q+ A+ F +  +              + + G+DGM                      +G            +LG G      E   G         + K+                         C+ CR S   +D   LG+  F +RS VL    P G+   CP                                                     G+ +Q CGHALHY CFD Y+A+++  S++  N++ +V+ GE+ CP CKAV   +VP  +P  ED
Sbjct:  249 PHRICQFAFKRNDIVWICKDCQKDETCVLCNDCFRLSQHQGHEVYFY--QTQAGGCCDCGDDDAWDKAGFCPRHGQDGNDPLSHLPPSFIAGSRWTMAQSVLFLRQAVAALQESYDLEHVEDAVWAGEAAAPGPGEPGEYYLTLHND-------------DMHSKREVVEVLQQCLGLNLQTARVLTDVVQEQGE-----------------------------------ARLHFGS------------------------LEEVRPKARRLQARSLSVSL----VTRRLHKIEKVAVLLVQWLYTLAGISGGTCRLVVEQLQPSTLNRLMVAHGHLPKPLATALHALYLFLMADQSFKKAVAQSYADTYLTVTSYYAAGIGVAESALYTLSVQFLNRSAFVNDLVGHRHFLEVLVQALADMIQRASGPSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSLSFSSHSRGISGN------LLVSHPIFRHRRYSPVVADLKFVLNIPGVSAHFANTCMDVWLSRLLIPLQYIHAQEREADEHVTYESREWMYAFNVQISLCHVLDCLLSW----VRTAPLGV--------ATAAQTESHGIKDTDMDVPVRLAKQEEWQERG-----------DKGVGTSDFGSQASVVVAGI-------------------QILGAIKSWQETFCKAFLSQGVGIAREVWEEAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSERG------------FSAASSSLEPPTPLL----PGPAAEATVSY-----PCHPKGTLHLLRLPLRRSFHLILHRAFAFLVREAVKNPALLSSTSAPSEGDLGQLLAHISEEPLLALGLFEMPVSVLVLSAEIRAGLWRRNGQLMMDQVINYREPPFCRVFRDMDLLLVQMAALAWGDWEKVMNALLRRFHVWTWLPPAGWEDVGKADEAGGQGGDRKA----------------------------------------------------------------TDSSSWMRPEGGQRPRSGRPWNRRDRWDDDVVRETLLPLGEECLLLLILLITEVPLPPMP-----------------------------------------------PQD------------RHRLSLRREIVHRLASGPCTHSEVHECCHHSSDGSEVIPESMIEEILASVAVRREARD-LEAERFVLSESAWE-EYDPAFFHMTNQAHQQASEMRPSPKKESGPSPKACVRPPPPAHPVFAPLRLGLLSSRTLLQVIRTVLMKAFPP-SGRSSSGVPASTSFLSEPTEPTDSTGFIAPLPSPXXXXXXXSACIRDSLIPRCLHLLTLAVHAL----------------RDC--------------PSSP-----------------------------------------------ATRSFFAAMHDTSALPSSSSP------------SLFSLLLALPSSIASSSDVHLLQGTQWLLQNLGRLDPVCQ-------------AALDRS----------------------------------VAVGEGAQKIARSA-HGRKKTAREKMLAKMQRSAAEFLKGQEGKEEEEEDVPRRVRENKEGDDGM----------VDEDDRRAGKSAEGRIMPSPRKKSLGELGPGETNG-EEGMEGEEKAGGEEVLPKKPV-----------------------CMACRGSSAKEDSSPLGYAAFVQRSNVLSRSLPLGELDACPTSSSPQKPGRXXXXXADGKCGQEEEEGEXEGTIEGGRSAARSGKQAGFQERREGLIVQLCGHALHYGCFDAYFASVVESSEAMGNLIYNVQAGEYPCPYCKAVSTCLVP-HIPVVED 1706          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A6H5JVD3_9PHAE (E3 ubiquitin-protein ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JVD3_9PHAE)

HSP 1 Score: 270 bits (689), Expect = 4.490e-73
Identity = 233/464 (50.22%), Postives = 270/464 (58.19%), Query Frame = 0

Query: 1794 RLQCIVCRDSDDRGALGFVGFGRRSRVLDIRPEGDFRCPGVHLQFCGHALHYTCFDRYYATMLHISDSHNNMVLDVEKGEFHCPLCKAVGNLMVPCFVPADEDXXXXXXXXXXXXXXGALRVVGEGSMEGQAQSIPGRRGSGLGGSGSLVSGEPKLSGRKGARRDAHTRTGTKGEEEGRGSEPSAAVAAAAAVARDGDAMDVSDDERNPTNAAAGSGIDGGVGGCWLDTKAALSLEMAKSSAFATRRGAXXXXXXXXXXHERT----NRTGSRSGLLAMDGATDEGRGXXXXXXXXXXXXXXXXXGQFHGLNVETSSMRPQQQQQQATKRFLQSVYEVENDVTTVPPSVSTEELFLCACRALAYSVSSAAAATSPLESLRGHVAAAASTASGXXXXXXXXXXXXXXXXARVGAGGAAAWGGLDHADATRLRCLGLVAEALRKAAEQVEGLEETVRRKLAASLRG 2253
            R +CIVCRD  DRG LG+VGFGRRS+VLD+RP+G+ R PGVHLQFCGH LHY+CF++YY TM+ +SDS+NN+ LDV+KGEFHCP CKAVGNLMVPC   + E      XXXXXXXXX            G            LGG                             E  G+G         A A    GD MDVS++E   ++  AG   D  VG  WLD KAALSL  A+ S FA R+  XXXXXXXXXX        +RTGS    + M G  D    XXXXXXXXXXXXXXXXX     L+ +      QQ    AT++FL+SVYEVE  VT V PSV+ E LFL ACRALAYSVSSAA   SP++SLRG+  A A+ A+G                   G  GAAA   +DH DATRLRCL LVA  LR+AA Q  GLEETVRRKLA SL G
Sbjct:  157 RPECIVCRDDADRGPLGYVGFGRRSQVLDVRPDGEERSPGVHLQFCGHTLHYSCFEKYYVTMVQMSDSNNNVALDVDKGEFHCPFCKAVGNLMVPCLEESSETEDSDWXXXXXXXXXXXXXXXXXXXXVG------------LGGK----------------------------ERPGKG---------ATADHAGGDPMDVSEEEHQSSD--AGGDGDDDVG--WLDRKAALSLMTARPSMFAARQSXXXXXXXXXXXXXXXXXAMDRTGSGFVYMVMAGNADSXXXXXXXXXXXXXXXXXXXXXXXXELSAQ------QQLFDSATRKFLESVYEVETGVTPVLPSVTPEALFLSACRALAYSVSSAATTASPIDSLRGYATAVAAAAAGS------------------GTRGAAATA-IDHGDATRLRCLRLVAGVLRRAAGQA-GLEETVRRKLAGSLAG 541          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A1Z5JBN3_FISSO (E3 ubiquitin-protein ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JBN3_FISSO)

HSP 1 Score: 267 bits (682), Expect = 1.040e-67
Identity = 201/561 (35.83%), Postives = 274/561 (48.84%), Query Frame = 0

Query:  199 LPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHG----LDNRDPLHELPPGLITGGTKVMGAVQTFLL-------EATYALVRS----------FDLSQPWTSDRGRTPKALSVMIHHTVYRETSLS---EALASVAKIDVAKIHEVRWPLPSCDSPNTSRRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKA----DEAEVICFREGERGEEADRRELQRMLDIARGLSERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCD-LFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSVSGGAPGNQDGKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFL---RTCFTEWIKILVMVQQMAPQVRQQ--RAHVEREDRDWMFAFNINISLTSLFECMLGW 725
            +PRRVCQ+ F KNDIVW+C+ CQADETCVLC+ CF  SDH GH+V FYHAQ  AGGCCDCGDPDAWD  GFC  HG      + DP   LPP L+     V+ AV  +L+       E  YA V S           D+     S R       +   + +   E SLS   E   S+ ++         + +   D  +   +  +  +R + G +   S   L  M+ +  Q         +     S +   L S +A    D  +V C R GE            ML+ A  L   G+  S+ T +      +A+ + +WL  V+   D  C  V + +     LV L+ +D  +   +  A + L L L+A   FK+ +AAAY      VT  YARG+G  E + YTLSVQFLNR  +V DLV+   LL  L   L+ T+  A   Q  +G           L  +H V+AHRRY+P V+DLKCVLN+ G+P+       T   +W+  L + Q M PQ  +   + HVE E R W+ AFN +ISL SLFE +LGW
Sbjct:  143 VPRRVCQHPFRKNDIVWVCRTCQADETCVLCHTCFSQSDHAGHDVNFYHAQ--AGGCCDCGDPDAWDPAGFCPFHGPNAACSSSDP-GALPPSLVNRVRGVVPAVVDWLVLTIAENAEKAYARVNSGLPLDTDMEIMDVDDMMVSPRSHIFSPTAASTNRSQRDELSLSANNEVAHSIGQL--GSTGRGLFLVLHADDIHVKSQV-VEALRHFWGTSMFISDMLLNRMVDALKQ------YGQLVIWGTSDVCAGLTSAQAHLWMDGDKVACTRVGEI-----------MLERAYRLLRHGMFCSIVTYDELLLEQQAVGILQWLSIVARSCDPLCVEVAECILPNRHLVPLLRSDFKMSSRVTKAWYALLLTLLAVPTFKSHLAAAYCDTYADVTSKYARGMGVLERSGYTLSVQFLNRVTYVIDLVQSRDLLGKLGESLLETMKVATKSQQYNGR----------LDPNHFVLAHRRYSPCVSDLKCVLNVAGMPRVACCAKTTFLKDWVATLAIAQFMDPQSWRNWNQGHVEDESRGWVGAFNASISLGSLFERLLGW 670          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: B7GBX5_PHATC (E3 ubiquitin-protein ligase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7GBX5_PHATC)

HSP 1 Score: 245 bits (626), Expect = 2.050e-63
Identity = 208/620 (33.55%), Postives = 295/620 (47.58%), Query Frame = 0

Query:  183 SISTLADLQQKLLPPDLPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLH-ELPPGLITGGTKVMGAVQTFLL-------EATYALVRSFDLSQPWTSDRGRTPKALSVM-------------------IHHTVYRETS------------LSEALASVAKIDVAKI-------HEVRWPLPSCDSPN----------TSRRFALGGVRAWSG-----------ENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKADEAEVICFREGERGEEADRRELQR----MLDIARGLSERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCD-LFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSVSGGAPGNQDGKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFLR---TCFTEWIKILVMVQQMAPQVRQQRA--HVEREDRDWMFAFNINISLTSLFECMLGW 725
            S + LA LQ        PR+VCQ+ F KNDIVW+C+ CQADETCVLC++CF  S+H+GH+V FYHAQ  AGGCCDCGDPDAWD  GFC HHG +  +     LP G       V+ AV  +++       E  YA   +    +  +SD    P +L  +                   I   ++ ET+            LS+   SV  +D  ++       H      P+  S +           +R  ALG   A  G                 + AL   + S N   T  ++  +  A       L+  G  + A  + + + +   + D+    R    +L+ A  L+  G   S+ T E      +A+ V +WL  ++      C  V   +     LV L+EAD  +   +  A + L L L+A   FK+ +AAAY     SVT  YARG+G  E + Y LSVQFLNR  +V DLV+   LL  L   L+ TL  A  R+      P N +G+L    +H V+AHRRY+P ++DLKCVLN++G+P+ F     T   +WI++L + Q M PQ  +     HVE E R W+ AFN +ISL S+FE +L W
Sbjct:  129 SAAALAALQAA--GSSFPRKVCQHPFRKNDIVWVCRTCQADETCVLCHNCFSQSNHEGHDVAFYHAQ--AGGCCDCGDPDAWDPDGFCPHHGPNAVNASDARLPEGFTHRVQGVVPAVVDWMVRDVAAVGETGYARCHAPTPLRRASSDHEDLPPSLPSLAQRSASMPLEQELQSVGMDIDEELFAETAENDETAADDEDLLSDRFHSV--VDSLRMYGPSNFNHRTHVFSPTAASKSGAAADSLRRHEARAEALGRKGATGGGLFLVLHHDDVHTSQSWLDALREFLGSTNY-YTDTLLGKLIKA-LKTYGQLIVWGTMEVATEVGWTQVQLWMDGDKVASTRIGALLLEHASRLTRHGAFCSILTREELWLEQKAVGVLQWLSKLAESCHPFCNTVAACILPNRHLVPLLEADFKMSARVMKAWYSLLLTLLAVPTFKSHLAAAYCDTYRSVTAKYARGMGVLERSGYQLSVQFLNRVQYVVDLVQGRDLLGKLGKTLLDTLLVA--RR------PRNLNGRL--NPNHHVLAHRRYSPCISDLKCVLNVKGMPRLFAAKGGTFLGDWIEVLSIAQFMDPQSWRHWTFGHVEDESRGWVGAFNASISLGSIFERLLSW 730          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A1E7FD43_9STRA (E3 ubiquitin-protein ligase (Fragment) n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FD43_9STRA)

HSP 1 Score: 234 bits (598), Expect = 1.240e-60
Identity = 198/618 (32.04%), Postives = 278/618 (44.98%), Query Frame = 0

Query:  162 TADDVDLKQPSTALSGGGGIVSISTLADLQQKLLPPDLPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHG------LDNRDPLHELP----PGLITGGTKVMGAVQTFLLEATYALVRSFDLSQPWTSDRGRTPKALSVMIHHTVYRE-TSLSEALASVAKIDVAKIHEVRWPLPSCDSPNTSRRF-----ALGGVRAWSGENPACSVPALATMISS--------RNQDLTHPVVAVVACAN--------ASQIRYLLASGKADEAEVICFREGERGEEADRRELQRMLD---IARGL-------------SERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCDLFDVAT-LVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSVSGGAPGNQDGKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFLRT--CFT-EWIKILVMVQQMAPQVRQQ--RAHVEREDRDWMFAFNINISLTSLFECMLGW 725
            T DDV  + P  A         ++TL  L + +     PRRVCQ+ F KNDIVW+C+ CQADETCVLC+ C++ SDH+GH+V FYHAQ  AGGCCDCGDPDAWD  GFC  HG        NR PL         G++      M  V+   + A     R+ +  +   +    TP    V       ++ T +                       +     + RR      A G  R    + P      L  +           ++ D+      V A  +           I   +        +++ +   E   E    +++  LD   IA G+                GL  S+ T +  +    A+ V +WL A++   D  C+ V +       L  L++AD  L   +    H L L L+A   FK+ +AAAY     SVT  YARG+G  E + Y LSVQFLNR  +V DLV+   LL  L   L  TL       +V+     NQ  +L    +H V+ +RRY+P ++DLKCVLN++G+P+ F     CF  +WI  L + Q M  QV +   + HVE E R W+ AFN +ISL SLFE +L W
Sbjct:   44 TGDDVQYESPRDA---------VNTLDTLSRSV-----PRRVCQHPFKKNDIVWVCRTCQADETCVLCHACYKGSDHEGHDVAFYHAQ--AGGCCDCGDPDAWDPAGFCSRHGPAASVGSSNR-PLSGSVIFRVQGMVPAAVDWM--VKYVAMTAIEGFERTEEEQEQLHTQPTETPTQSRVEEGDVEMKDSTDVVTXXXXXXXXXXXXXXXXXXXXKTASGRGSVRRLIFSPSAAGSSRRQQKDLPYTKAEELGMLAKRGGGLYLVLKSDDIHTDRQLVEAFRDLLGTSSLYTESILQKVVQALKQFGQLVVWGTTEILAELSTTQIRLWLDGDRIASGVFGSAILNRAKILTRHHGLFCSILTRQELQIEQHAVTVLQWLTALARSCDPLCQKVAESISPDRHLAPLLQADFKLSSRVTKYWHSLLLTLLAVPTFKSHLAAAYCDTYQSVTAEYARGMGVLERSGYALSVQFLNRVTYVVDLVQRRDLLGKLGASLFQTL-------NVAAVPVDNQQRRL--NPNHFVMMNRRYSPCISDLKCVLNVKGMPRLFASKSGCFLKDWIAALGLGQMMDSQVWRDWTQGHVEVEHRGWVGAFNASISLGSLFERLLSW 633          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A7S1ZF23_9STRA (E3 ubiquitin-protein ligase n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZF23_9STRA)

HSP 1 Score: 238 bits (607), Expect = 6.200e-59
Identity = 246/946 (26.00%), Postives = 383/946 (40.49%), Query Frame = 0

Query:  199 LPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLHELPPGLITGGTK-VMGAVQTFLLEATYALVRSFDLSQPWTSDRGRTPKALSVMIHHTVYRETSLSEA--LASVAKID-----VAKIHEVRWPLPSCDSPNTSRRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRY----LLASGKADEAEVICFREGERGEEADRRELQRMLDIARGLSERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCDLFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGR-------------QSVSGGAPGNQDG-KLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQ------HFLRTC-----------FTEWIKILVMVQQMAPQVRQQ--RAHVEREDRDWMFAFNINISLTSLFECMLGWLREPVRAAPIPAGFLSWEKPAAAAXXXXXAITHNRTSTSPSHADSEINGRRGGVXXXXXXXXXXXXXXXXXXGARPSAPPAGLIGQPSFVTFPPXXXXXXXXXXXXXSPSLSP--TQAELFAAEGGMPPSPWTAAGANAAMAAFLSEARRHVEGSAAPAMAAAAAATSQASTSIHPASEAVGAARSGRGGAEVVAWEHQALPAYPAGVLWVRPPVAGRSFHIILHRFFAAMVREACHCE------ERERDLS-----------ALSSYVTCY-----PEVATDVADTALNVLSFAAEVRAGLWRKNGQCMNDQVVNYSEPSFCKTFRDLDLLTVQFAGMS-----------------CGATQLVNHILHKFQCF 1058
            +PRRVCQ AF KNDIVW C+ CQ D TCVLCNDC+ SSDH GH+V FY AQ  +GGCCDCGD DAWD +GFC  HG    D   E+       GT+ V  A+  +++      V S                     +H    +E  +  A  L ++ +       V    +V W        +  R  AL  +  +S  +P          +   ++++   +  ++  ++   I +    L+     + A++  ++EGE  E A R+  + ML  +R L+ RGL+ S++T     R  R     EWL+AV+   D     V        L  ++  +  LP+ +A A + L L L+A   FK  +A AYV     V   YARG+G+  ++ Y LSVQFLNRA++VK+L     L++ + G  +  L  A                +S++ G  G+    + ++   H+V+   RY+PI++DL+ +L++ G+ +      +    C              WIK L + Q M  QV +   + HVE E + WM A+ +++SL  +FE +L W  E     PI     S E+   +       I      T  +   SE+N  +                           P A +   P                     PS  P  T A  +              G++ AM                                                         +ALP     V  VRP     SFHI LHRF A  +RE           E+ +D++            L+S ++ +           + +  + VLS  A++R+GLW++NG CM++QV  Y E  +C++ +D DL+ +QFA ++                  G   + N +LH+F  F
Sbjct:  189 IPRRVCQKAFRKNDIVWACRTCQTDRTCVLCNDCYTSSDHDGHDVVFYRAQ--SGGCCDCGDADAWDPKGFCPRHGAGATDEAIEMLGNSKAEGTRGVAEAIMDWIVRELLGGVES--------------------ALHRVSGQENIIDPARHLGNIGRQQSGLYLVLHADDVSW--------SEDRSTALRELYTYSQVDPNRPSFVPPRNVFPHSEEVIFQIATLLFRSHGDLIMWGTNELIREAGHETAKL--WKEGE--EVATRQIGELMLHRSRLLNHRGLVCSIKTHSELLREGRVAAALEWLNAVAQSCDPLSLQVGSAVS-GRLPPMLRCNLFLPRRIASAWNSLLLTLVAAPSFKGALAGAYVDAYSDVASEYARGIGSHHWSSYALSVQFLNRASYVKELAREKRLMETVVGAFLDMLRLAAKEIHSDSSSSDWSKTESLNDGKTGHSPRIQYIVNPLHTVLTQYRYHPILSDLRLILDVPGIARLVSLVPNATENCDGNKPLGHLSFLDSWIKTLSLCQNMDGQVWRSWGQTHVENEHQSWMGAYMLSVSLGDIFERLLAW--EDDDPCPINIKNKSKEECLLSVSELTVYIMSTGLDTWQT---SEMNSYK---------------------------PTANIFDIPQ----------------PRRCPSSLPYSTVAVDY--------------GSDLAM---------------------------------------------------------EALP-----VAQVRP----FSFHIPLHRFVAVCLREVARRPNARVGTEKNKDVTNQVGKEDGIHLVLTSLLSSFGSQRLKRFLRGLVEFPVIVLSRVAQIRSGLWKRNGYCMSEQVGRYDEQEYCRSMKDADLMLLQFAIIAHQALHSFGSVDDTYVNDAGCAYITNLLLHRFGLF 971          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A7S1XL72_9STRA (E3 ubiquitin-protein ligase (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XL72_9STRA)

HSP 1 Score: 218 bits (554), Expect = 8.310e-57
Identity = 156/467 (33.40%), Postives = 215/467 (46.04%), Query Frame = 0

Query:  158 FLSITADDVDLKQPSTALSGGGGIVSISTLADLQQKLLPPDLPRRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLHELPPGLITGGTKVMGAVQTFLLEATYALVRSFDLS-------QPWTSDRGRTPKALSVMIHHTVYRETSLSEALASVAKIDVAKIHEVRWPLPSCDSPNTSRRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKADEAEVICFREGERGEEADRRELQRMLDIARGLSE---RGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCDLFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTL 614
            +LS      + + P  A +  G      T    + + L     RRVCQYAF +NDIVWICK CQADETCVLCNDCF  S+H+GHEVYFYHAQ  AGGCCDCGD  AW+  G C  HG  N DPL  LPP  +   ++VM     ++L        + D++       +P  SD  R              R         ++ + ++ + +++   L   D P      A   +R   G   AC                                  LL  G  +E   +  R            L R +   R  ++   +GL+  + T E+         V ++LH  + LSDG CRLV   F+   L  L E DA LP+P    L+ LYL LM+D PFK  +A AY R  P++T ++ RG G  + ++Y+LSVQFLNRA FV  L ++    ++LA  L  +L
Sbjct:   73 WLSAACVGYEEESPRPAPADAGAATEEDTRRKARFEALSRLSERRVCQYAFKRNDIVWICKECQADETCVLCNDCFIDSEHEGHEVYFYHAQ--AGGCCDCGDQYAWNPAGCCSRHGKSNSDPLRHLPPPFVAACSEVMRLCVAYVLRYARHARLAHDIARGLARTGEPPPSDDWRR------------LRTPGDDGYYCALLEDEIHQDNQIMMALSLTDLPGRGSIIAEC-LRLLRG---ACGF-------------------------------VLLRCGSMEEVLPLAGR------------LARPISDPRATTDPKLKGLVTVITTRELMSMRLVVQAVIKFLHRSAELSDGVCRLVAREFNSEVLGKLFELDAYLPRPAKETLNALYLHLMSDAPFKRLLAGAYARSFPTITAAFQRGYGLIDTSIYSLSVQFLNRAMFVASLRQLKDFYRVLAAGLYRSL 478          

BLAST of mRNA_P-fluviatile_contig8.14436.1 vs. uniprot
Match: A0A835YQN3_9STRA (E3 ubiquitin-protein ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQN3_9STRA)

HSP 1 Score: 224 bits (570), Expect = 1.010e-54
Identity = 173/568 (30.46%), Postives = 241/568 (42.43%), Query Frame = 0

Query:  179 GGIVSISTLADLQQKLLPPDLP---------RRVCQYAFLKNDIVWICKACQADETCVLCNDCFRSSDHKGHEVYFYHAQVSAGGCCDCGDPDAWDSRGFCHHHGLDNRDPLHELPPGLITGGTKVMGAVQTFLLEATYALVRSFDLSQPWTSDRGRTPKALSVMIHHTVYRETSLSEALASVAKIDVAKIHEVRWPLPSCDSPNTSRRFALGGVRAWSGENPACSVPALATMISSRNQDLTHPVVAVVACANASQIRYLLASGKADEAEVICFREGERGEEADRRELQRMLDIARGLSERGLLISVRTDEVTRRMDRALDVAEWLHAVSALSDGCCRLVCDLFDVATLVALMEADACLPKPLACALHDLYLALMADQPFKTRIAAAYVRVLPSVTESYARGVGTAEFALYTLSVQFLNRAAFVKDLVEMHHLLQILAGCLVSTLAPACGRQSVSGGAPGNQDGKLVLKASHSVVAHRRYNPIVADLKCVLNIEGVPQHFLRTCFTEWIKILVMVQQMAPQVRQQRAHVEREDRDWMFAFNINISLTSLFECMLGWLREPVRAAPIPA 737
            GG++  S L  L Q    PD           RRVCQYAF KNDI                                                DA DS GFC  HG  + DP+  L P  ++    V+  V  FLL+  + +  S DL+                      + E + +EAL +     V  +H         D  +    F   GV     E    S          RN   ++              + LL  G A E+                    RM+ +A+ ++ R  + ++R               +WLH +S +SD  C L+C       L  L+ AD  LP+ L+ ALHDLYL LMAD  FK  IA AYV  LP++TE Y  G G AE+++ +LSVQFLNR+ FV+ LV+ + LL  LA CL  +L P  G    S  + G+ +  LVLK       +RRY P   DL+CVL I+G+ + F       W++ L ++Q +  Q RQ  AHVE E R W+ AFN+NI++ S F+ ++ WL +PV     PA
Sbjct:   61 GGVLRYSALDGLLQTDRNPDSALEWLGGAGMRRVCQYAFTKNDI------------------------------------------------DALDSAGFCTRHGGPSADPIKSLNPAFVSAAQTVLSVVVGFLLDCAHQIPSSADLA---------------------AHSEGAFAEALGASDGDVVVVLHH--------DDVHNRTEFEACGVALMDREPTQMSA-------DFRNNGESYAS------------KSLLPEGSAGESHY-----------------DRMVALAKEMTRRTFMATIRAXXXXXXXXXXXXXXKWLHKLSLVSDATCALICQQLQRPQLDVLLAADVYLPRRLSAALHDLYLTLMADAGFKMDIAEAYVSALPALTEHYVEGRGVAEYSIQSLSVQFLNRSTFVQHLVQHNGLLPTLAQCLYDSLFPIAGFD-FSQASDGSDNYPLVLK-------YRRYMPTFGDLRCVLAIDGIARQFAGEGLELWLQSLALLQGLDGQRRQTHAHVEYEHRSWVHAFNVNITVCSAFDLIVSWLHDPVAGIDYPA 507