prot_P-fluviatile_contig65.13156.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: D7FL91_ECTSI (Asparagine synthase (Glutamine-hydrolyzing) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FL91_ECTSI)

HSP 1 Score: 1002 bits (2590), Expect = 0.000e+0
Identity = 478/555 (86.13%), Postives = 525/555 (94.59%), Query Frame = 0

Query:   31 LRHRGPDWSGYEIVTVSEKRRHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILADEEPLTGSDCEAIIPLYLKKGGDFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSKTGEFTQWYSPVWRSIKEHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPGKIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADFEIGEDGAKKAAGIANATEA 585
            LRHRGPDWSGYEI++VSEKR+HGIGHERLSIIDPESGAQPLFSRDGQVIL+ANGEIYNYQ++L+DEEPLTGSDCE+IIPLY+KKG DFLNDLRGMFAFFIYDKRDDSFFVARDHVGI+PLYIGWGDDGSVW+SS  K AL+ GCR+L+AFPPGH+YSSKTG+FTQWY+PVWR++KEHPT+RVDL R+R++FT AV +RMMTDVPWGVLLSG GLDSSLVASVACR++RDHPDS  FS+FP+IHSFSVGLA SPD+KAA+EVA FL TVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRA+TPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAP  QEFFEETRDKVLALHQFDCARANKSTA+WGVEARVPFLDVDFIEE MMT+PEDKM+ PGKIEKYI+RKAFDTP+D YLPQHILYRQKEQFSDGVGYNWID+LRAKA  EV+D QF+HRANRFPRDPPATKEAYLYR +FEE FP+ AA ETVPMGKSIACST+RALEWDESFKNRADCSGRA+ GVHDDAYG +FE+G DGAKKA G+A+ATEA
Sbjct:   28 LRHRGPDWSGYEIISVSEKRKHGIGHERLSIIDPESGAQPLFSRDGQVILSANGEIYNYQELLSDEEPLTGSDCESIIPLYMKKGTDFLNDLRGMFAFFIYDKRDDSFFVARDHVGIIPLYIGWGDDGSVWISSEMK-ALVQGCRSLRAFPPGHSYSSKTGDFTQWYNPVWRTLKEHPTQRVDLARLRESFTTAVVRRMMTDVPWGVLLSG-GLDSSLVASVACRHVRDHPDSGRFSAFPRIHSFSVGLAGSPDIKAAKEVADFLGTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRASTPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPNGQEFFEETRDKVLALHQFDCARANKSTASWGVEARVPFLDVDFIEEAMMTNPEDKMVAPGKIEKYIMRKAFDTPEDVYLPQHILYRQKEQFSDGVGYNWIDELRAKAAEEVTDEQFKHRANRFPRDPPATKEAYLYRKIFEEFFPTPAAAETVPMGKSIACSTARALEWDESFKNRADCSGRAIGGVHDDAYGDEFEVGADGAKKATGVAHATEA 580          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A835Z2W5_9STRA (Asparagine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z2W5_9STRA)

HSP 1 Score: 771 bits (1991), Expect = 2.470e-275
Identity = 385/543 (70.90%), Postives = 437/543 (80.48%), Query Frame = 0

Query:   31 LRHRGPDWSGYEIVTVSEKRRHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYN----YQDILADEEPLTGSDCEAIIPLYLKKGGDFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSKTGEFTQWYSPVWRSIKEHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMI--VPGKIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADFE 567
            LRHRGPDWSGY IV      +HGI HERL+IIDPESGAQPL S DG V+LA NGEIYN    YQ++     P TGSDCEAIIPLY ++G DF+NDLRGMFAF IYD +  +FF  RDHVGIVPLYIGWGDDGSVW++S  K AL   CR  + FPP H +SS T EFT+WY+P WR++   PT+  DLT +R+ FT+AV +RMM+DVPWGVLLSG GLDSSLVASVA R+ R +P+    S FP+IHSFSVG+  SPDLKAA+EVA FL TVHHSY YT+QEGLDAIREVV  +ETYDTTTIRA+TPMFLMSRKIKAMGVKMVLSGEGADE+ GGYLYFHKAP  QEFFEETRDKV ALHQFDC RANKST+AWGVE RVPFLD DF+E  M   P +KMI    G++EKYI+RKAFDTPDDPYLP HIL+RQKEQFSDGVGY WID LR  A A VSD  F HRANRFP +PPA+KEAYLYR++FEE +P+QAA ETVP G SIACST+RA+EWDESFK RADCSGR+V GVH+ AY   F+
Sbjct:   28 LRHRGPDWSGYRIVGNG---KHGIAHERLAIIDPESGAQPLTSHDGTVMLAVNGEIYNFKELYQNLHKSYTPKTGSDCEAIIPLYEQRGMDFINDLRGMFAFVIYDAKTGAFFAVRDHVGIVPLYIGWGDDGSVWIASEMK-ALAVECRRFRPFPPAHYWSSTTKEFTRWYNPGWRTLTGMPTQPCDLTALRNAFTKAVVRRMMSDVPWGVLLSG-GLDSSLVASVASRHARRNPN----SPFPRIHSFSVGIEGSPDLKAAKEVADFLGTVHHSYQYTIQEGLDAIREVVYHLETYDTTTIRASTPMFLMSRKIKAMGVKMVLSGEGADEIFGGYLYFHKAPNAQEFFEETRDKVQALHQFDCLRANKSTSAWGVEPRVPFLDADFLELSMNIDPSEKMIHKAEGRMEKYIIRKAFDTPDDPYLPDHILWRQKEQFSDGVGYGWIDALRDNAAAHVSDDMFNHRANRFPHNPPASKEAYLYRSIFEEFYPTQAAAETVPGGPSIACSTARAIEWDESFKGRADCSGRSVGGVHNMAYDETFK 561          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A7S1TW89_9STRA (Hypothetical protein n=2 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TW89_9STRA)

HSP 1 Score: 679 bits (1752), Expect = 9.110e-239
Identity = 345/551 (62.61%), Postives = 403/551 (73.14%), Query Frame = 0

Query:   31 LRHRGPDWSGYEIVTVSEKRRHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDIL-------ADEEPLTGSDCEAIIPLYLKKGGDFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSKTGEFTQWYSPVWRSIKEHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSF-PKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIV--PGKIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADFEIGED 571
            LRHRGPDWSGY+I        HGI HERL+IIDPESG QPL S  G VI+ ANGEIYNY+ +         +  P TGSDCE +IPLY K G  F++ LRGMF+F IYDK  D FF  RDH+GI P+Y G   DGS+W SS  K AL   C T +  PPG  +SS+ GEF QW++P W +  E P+  + L  +RD F  AV +RMM+DVPWGVLLSG GLDSSLVAS+ACR  +   +S   +    ++HSF++GL  SPDL AAE+VA FL TVHH Y YT+QEGLDAI EV+  +ETYD TT+RA+TPMFLMSRKIKAMGVKMVLSGEGADE+LGGYLYFH AP   EFFEETRDKV  LH++DC RANKST+AWGVE RVPFLD DF+   M   PE KM    P KIEKYILRKAFDTP++P+LPQHIL+RQKEQFSDGVGY WID LR  AEAEV+D Q  + A+RFP + PATKEAY YRA+FE  +PS AA  TVP   SIACST+RA+ W ESF  RAD SGRAVAGVHD  Y  +F+I +D
Sbjct:   28 LRHRGPDWSGYQI-----HGSHGIAHERLAIIDPESGEQPLVSPCGNVIVCANGEIYNYRTLYDELKADGCEYNPATGSDCEVVIPLYQKYGMAFVDKLRGMFSFVIYDKAADKFFAVRDHMGITPMYYGHASDGSIWFSSEMK-ALHDQCSTYQQAPPGKYFSSEDGEFHQWFTPDWLTTTESPSETIHLAELRDYFVNAVRRRMMSDVPWGVLLSG-GLDSSLVASIACRLHKQGSNSGAKAFVGARLHSFTIGLEGSPDLAAAEDVADFLGTVHHGYTYTMQEGLDAISEVIYHLETYDVTTVRASTPMFLMSRKIKAMGVKMVLSGEGADEILGGYLYFHHAPNGSEFFEETRDKVARLHKYDCLRANKSTSAWGVEVRVPFLDADFMNYAMGLEPECKMPAKAPRKIEKYILRKAFDTPEEPWLPQHILWRQKEQFSDGVGYGWIDSLRDVAEAEVTDAQMANAAHRFPYNTPATKEAYRYRAIFESHYPSHAAATTVPAEASIACSTARAMSWHESFSKRADPSGRAVAGVHDSEYSENFDIEDD 571          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A7S2YB47_9STRA (Hypothetical protein n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S2YB47_9STRA)

HSP 1 Score: 676 bits (1744), Expect = 2.540e-237
Identity = 336/562 (59.79%), Postives = 415/562 (73.84%), Query Frame = 0

Query:   31 LRHRGPDWSGYEIVTVSEK----RRHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILA----DEEPLTGSDCEAIIPLYLKKGG--DFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSK---TGEFTQWYSPVWRSIK----EHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPG--------KIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADFE 567
            LRHRGPDWSGY+++           HGIGHERL+IIDPESG+QPL S D  VILAANGEIYNY+D+ +    D +P TGSDCE I+PLY + G   D    LRGMF+F +YD+R+DSF VARDH+GI PLYIGWG+DGS+++SS  K  +I  C   + FPPGH Y SK    G+F +W++P W        + PT+  D  R+R+ F +AV +RMM+DVPWGVLLSG GLDSSLVA++  R++         ++FPK+HSF++GLANSPDL AA++VA  L T+HH+Y YT++EG DAIR+V+R +ETYD TTIRA+TPM+LMSRKIKAMG+KMVLSGEGADE+ GGYLYFHKAP  QE F+ET DK+  LH +DC R NK+ +AWGVE RVPFLD DF++  M   P++KMI+ G        KIEK+ +RKAFDTPDDPYLP  +L+RQKEQFSDGVGY W+D L+  AE E+SD  F   ANRFP + P TKE Y YR +FEELFP +AA +TVP GK+IACST RA+ WD+SF  RAD SGRA AGVHD AY A FE
Sbjct:   28 LRHRGPDWSGYKVIEADPSMGIPNTHGIGHERLAIIDPESGSQPLVSPDSSVILAANGEIYNYKDLYSTLTVDYKPKTGSDCEVILPLYEQFGATIDLPRQLRGMFSFILYDRRNDSFVVARDHIGITPLYIGWGNDGSIYVSSEMKS-MIGECTKFQQFPPGHLYCSKGPHAGKFQRWFTPSWAPEMLPGIQMPTQPYDAERLRNAFEKAVIRRMMSDVPWGVLLSG-GLDSSLVAAICARHIGRRT-----TAFPKLHSFTIGLANSPDLLAAKKVADHLGTIHHAYTYTIEEGADAIRDVIRSVETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAQELFDETVDKISRLHMYDCLRCNKAMSAWGVEPRVPFLDADFMDVAMELDPQEKMIIKGPDVSKADTKIEKWAMRKAFDTPDDPYLPHEVLWRQKEQFSDGVGYGWVDHLKDVAENEISDQMFAQAANRFPHNTPTTKEGYRYRMIFEELFPGEAAEKTVPGGKTIACSTERAMNWDKSFATRADPSGRA-AGVHDSAYDATFE 581          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A4D9CNJ7_9STRA (Glutamine amidotransferase type-2 domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CNJ7_9STRA)

HSP 1 Score: 670 bits (1728), Expect = 5.680e-235
Identity = 340/545 (62.39%), Postives = 403/545 (73.94%), Query Frame = 0

Query:   31 LRHRGPDWSGYEIVTVSEKRRHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILAD------EEPLTGSDCEAIIPLYLKKGGDFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSKT---GEFTQWYSPVWRSIKEHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPG----KIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAY 562
            LRHRGPDWSGY++   +      I HERL+IIDP+SG+QPL S DG +I+AANGEIYNY+++  +        P TGSDCE IIPLYLK G DF   LRGMF+F +YD+RDDSFFV RDH+GI P+Y G+G DGS+W +S  K AL      +  FPPGH Y S+    G F QWY+PVW      PT RV+L  IR+TF  AV +RMM+DVPWGVLLSG GLDSSLVAS+A R     P+    + +P++HSFS+GL NSPDL AA EVA+F+ TVHHSYV+TVQEG+DA+REV+  +ETYD TT+RAATPMFLMSRKIKA GVKMVLSGEGADE+ GGYLYFHKAP  +EF  ET DK+  L Q+DC RANK+T+AWGVE RVPFLD DF++  M  +PEDKMIV      ++EK+ILRKAFD P DPYLP  IL+RQKEQFSDGVGY WID LR  A A+VSD  F    NRFP + P TKEAY YR +F+  FPS +A  TVP G SIACST RA+EWD SF+ RAD SGRAVA VH+ AY
Sbjct:   28 LRHRGPDWSGYKVYGSN-----AIAHERLAIIDPDSGSQPLVSPDGNIIVAANGEIYNYKELYEELGGIDFYTPKTGSDCEVIIPLYLKHGRDFPRFLRGMFSFVLYDRRDDSFFVLRDHMGITPMYAGYGADGSIWFASEMK-ALTDVVARVDVFPPGHAYDSELKGKGGFVQWYNPVWYDPTYLPTERVELLNIRNTFEMAVKRRMMSDVPWGVLLSG-GLDSSLVASIASRLSGRDPN----NEWPRLHSFSIGLENSPDLAAAAEVAKFVGTVHHSYVFTVQEGMDAVREVIYHLETYDVTTVRAATPMFLMSRKIKARGVKMVLSGEGADEIFGGYLYFHKAPDSKEFHAETVDKLRGLFQYDCLRANKATSAWGVEVRVPFLDQDFLDVAMTINPEDKMIVKDGEGRRMEKWILRKAFDDPADPYLPAKILWRQKEQFSDGVGYGWIDALRDHANADVSDKVFSAAENRFPYNTPLTKEAYYYRDIFDRFFPSPSAAATVPGGPSIACSTPRAMEWDASFRGRADPSGRAVAAVHESAY 561          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A7S3Q388_9STRA (Hypothetical protein n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3Q388_9STRA)

HSP 1 Score: 666 bits (1718), Expect = 2.660e-233
Identity = 336/571 (58.84%), Postives = 414/571 (72.50%), Query Frame = 0

Query:   22 KNAIPVPFSLRHRGPDWSGYEIVTVSEKR----RHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILAD----EEPLTGSDCEAIIPLYLKKGG--DFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSK---TGEFTQWYSPVWR-----SIKEHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPG--------KIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADF 566
            K  I     +RHRGPDWSGY +V+  E       HGI HERL+I+DPESG QPL S DG VI+AANGEIYNY+D+ ++     +P TGSDCE I+PLY ++G   DF   LRGMF+F +YDK  DSFF+ RDH+GI PLYIGW +DGSV++SS  K A++  C   + FPPGH +++K     +FT+WY P W       +K  PT       +R  F +AV +RMM+DVPWGVLLSG GLDSSLVAS+  R+++        +SFPK+HSF++GL NSPD+ AA++VA +L T HH+Y YT+ EG D++REV+R +ETYD TTIRA+TPM+LMSRKIKAMG+KMVLSGEGADE+ GGYLYFHKAP  QEF++ET DK+  LH +DC R NK+ +AWGVE RVPFLD DF+E  M  +PE+KMI  G        +IEK+ +RKAFDTPDDPYLPQ IL+RQKEQFSDGVGY W+D L+A AE EVSD  F + ANRFP + P TKE Y YR +FEE FP +AA +TVP GKSIACST RA+ WD+SF  RAD SGRA AGVHD AY   F
Sbjct:   19 KQLITCSTKIRHRGPDWSGYIVVSKDEAAGIPMNHGIAHERLAIMDPESGEQPLVSADGDVIVAANGEIYNYKDLYSELKEPYKPKTGSDCEVILPLYKEQGAGIDFPKRLRGMFSFILYDKAKDSFFICRDHIGITPLYIGWANDGSVYVSSEMK-AIVGQCNKFQNFPPGHIFTNKGPNANQFTRWYEPSWAPKLLPGLKP-PTVPYQPDMLRTAFEKAVVRRMMSDVPWGVLLSG-GLDSSLVASICSRHIKRRS-----TSFPKLHSFTIGLENSPDILAAKKVADYLGTTHHAYTYTLDEGADSVREVIRSLETYDLTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAQEFYDETVDKISRLHMYDCLRCNKAMSAWGVEPRVPFLDADFLETAMNLAPEEKMIKKGEGISAADTRIEKWAIRKAFDTPDDPYLPQEILWRQKEQFSDGVGYGWVDHLKAIAEKEVSDQMFGNAANRFPHNTPTTKEGYRYRMIFEECFPGEAAEKTVPGGKSIACSTERAMNWDKSFAGRADPSGRA-AGVHDSAYDQKF 580          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: B8BZR9_THAPS (Asparagine synthase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BZR9_THAPS)

HSP 1 Score: 660 bits (1703), Expect = 3.280e-231
Identity = 328/554 (59.21%), Postives = 402/554 (72.56%), Query Frame = 0

Query:   30 SLRHRGPDWSGYEIVTVSEKR----RHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDIL----ADEEPLTGSDCEAIIPLYLKKGG--DFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSK---TGEFTQWYSPVWRSIK----EHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPGKIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADF 566
            SLRHRGPDWSGY +V           HGI HERL+I+DPESG+QPL SRDG VI+AANGEIYNY+++     +   P TGSDCE I+PL+ + G   +  N LRGMF+F +YDK  D+FF+ RDH+G  PLYIGWG+DGSV++ S  K  ++  C   + FPPG  Y++K    G+F +WY P W          P  R     +R  F +AV +RMM+DVPWGVLLSG GLDSSLVAS+  R +         SSFPK+HSF+VGL  SPDLKAA++VA FL T+HH+Y YT++EG DAIREV++ +ETYD TTIRA+TPM+LMSRKIKAMG+KMVLSGEGADE+ GGYLYFHKAP  QEFF+ET DK+  LH +DC R NK+ +AWGVE R PFLD DF++  M   P++KMI   +IEKY++RKAFDTPDDPYLP  IL+RQKEQFSDGVGY W+D L+A A+ EV+D  F   ANR+P + P TKE Y YR +FEE FP ++A +TVP GKSIACST RA+ WD +F  RAD SGRA AGVHD AYG DF
Sbjct:   27 SLRHRGPDWSGYIVVKSDPSAGIPLNHGIAHERLAIMDPESGSQPLVSRDGNVIVAANGEIYNYKELFKALTSPYTPATGSDCEVILPLWAEFGASIELPNKLRGMFSFILYDKSSDTFFICRDHIGKTPLYIGWGNDGSVYVGSEMKS-IVGECSKFQNFPPGCCYTNKGPNAGKFMRWYQPNWAPEMLPGLAPPKDRFQADVLRHAFEKAVTRRMMSDVPWGVLLSG-GLDSSLVASICARNVARRS-----SSFPKLHSFTVGLEGSPDLKAAKQVADFLGTIHHAYTYTIEEGADAIREVIKSVETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNEQEFFDETVDKISRLHMYDCLRCNKAMSAWGVEPRTPFLDADFLQVAMNMDPKEKMIEDTRIEKYVIRKAFDTPDDPYLPAEILWRQKEQFSDGVGYGWVDHLKAVADKEVTDQMFASAANRYPHNTPTTKEGYRYRMIFEEFFPGESAEKTVPGGKSIACSTERAMNWDATFATRADPSGRA-AGVHDSAYGGDF 572          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A1E7F5L7_9STRA (Asparagine synthase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7F5L7_9STRA)

HSP 1 Score: 655 bits (1691), Expect = 2.770e-229
Identity = 327/562 (58.19%), Postives = 404/562 (71.89%), Query Frame = 0

Query:   31 LRHRGPDWSGYEIVTVSEKR----RHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILADEE----PLTGSDCEAIIPLYLKKGG--DFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSK---TGEFTQWYSPVWRSIK----EHPTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPG--------KIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADFE 567
            LRHRGPDWSGY ++     +     HGIGHERL+I+DPESG+QPL SRDG V++AANGEIYN++++    E    P+TGSDCE I+PLY + G   + + +LRGMF+F +YDK  D++ VARDH+GI PLYIGWG+DGSV++SS  K  LI  C   + FPPGH Y SK    G+F +WY P W          P        +R  F +AV +RMM+DVPWGVLLSG GLDSSLVA++  R MR      L S+FPK+HSF+VGL  +PD+ AA++VA  L T+HH Y YT++EG DAI +V+R +ETYD TTIRA+TPM+LM+RKIKAMG+KMV+SGEGADE+ GGYLYFHKAP  QEFF+ET DK+  LH FDC R NK+ +AWGVE RVPFLD DF++  M   P +KMI  G        +IEK+ +RKAFDTPDDPYLP  IL+RQKEQFSDGVGY W+D L+  AE E+SD  F +  NRFP + P TKE Y YR +FEE +P +AA +TVP GKSIACST RA++WD SF  RAD SGRA+A VHDDAY   FE
Sbjct:   28 LRHRGPDWSGYMVIEADASKGIPLAHGIGHERLAIMDPESGSQPLVSRDGNVVVAANGEIYNFKELYTALEKPYTPMTGSDCEVILPLYDQHGPTLEMIRNLRGMFSFILYDKSTDTYLVARDHIGITPLYIGWGNDGSVYVSSELKS-LIGECNKFQNFPPGHIYCSKGEHAGQFQRWYKPNWAPEMLPGGMMPREPYQADALRHAFERAVTRRMMSDVPWGVLLSG-GLDSSLVAAICSRQMR------LSSNFPKLHSFTVGLPGAPDIGAAKKVADKLGTIHHEYTYTLEEGSDAIPDVIRTLETYDLTTIRASTPMYLMARKIKAMGIKMVISGEGADEVFGGYLYFHKAPNEQEFFDETVDKLSRLHMFDCLRCNKAMSAWGVEPRVPFLDADFLQVAMNLDPNEKMIRKGPGISKADTRIEKWSIRKAFDTPDDPYLPDEILWRQKEQFSDGVGYGWVDHLKDIAEKEISDQMFANAPNRFPHNTPTTKEGYRYRRIFEEFYPGEAAEKTVPGGKSIACSTERAMKWDASFSTRADPSGRAMAAVHDDAYDGSFE 581          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: A0A7S2PGV7_9STRA (Hypothetical protein n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2PGV7_9STRA)

HSP 1 Score: 654 bits (1688), Expect = 8.480e-229
Identity = 338/586 (57.68%), Postives = 412/586 (70.31%), Query Frame = 0

Query:   22 KNAIPVPFSLRHRGPDWSGYEIVTVSEKR--RHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILADEE----PLTGSDCEAIIPLYLKKGG----DFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSK---TGEFTQWYSPVWRSIKEH----PTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPG--------KIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADF------EIGEDGAKKA 576
            K  I     LRHRGPDWSGY +V    +R   HGI HERL+I+DPESG QPL S DG +I+AANGEIYNY++I A       P TGSDCE ++PL+ +  G    +  N LRGMF+  +YDK  D++F+ RDH+G  PLYIGWG+DGSV++ S  K  ++  C   + FPPGH YS+K    G+F +WY P W    +     PT +     +R  F +AV +RMM+DVPWGVLLSG GLDSSLVAS+  R +         SSFPK+HSF+VGL  SPDLKAA+EVA FL T+HH+Y YT++EG DAIREV+R +ETYD TTIRA+TPM+LMSRKIKAMG+KMVLSGEGADE+ GGYLYFHKAP  QEF++ET DK+  LH +DC RANKS +AWGVE R PFLD DF++  M   P++KMIV G        +IEK+ +RKAFDTPDDPYLP +IL+RQKEQFSDGVGY W+D L+  AE EVSD  F + ANR+P + P TKE Y YR +FEE+FP +AA  TVP GKSIACST RA+ WD SF  RAD SGR  AGVHD AY  +F      E  E  +KKA
Sbjct:   19 KELIKCSSKLRHRGPDWSGYLVVEKDGERPLNHGIAHERLAIMDPESGEQPLVSPDGNIIVAANGEIYNYKEIYAGLSAPYTPKTGSDCEVMLPLWSEMDGIPTPNLPNQLRGMFSVIMYDKTTDTYFIFRDHIGKTPLYIGWGNDGSVYVGSEMKS-MVHECNKFQNFPPGHCYSNKGPNAGQFVRWYKPNWAPEMKPGLPPPTEKYQADLLRHAFEKAVVRRMMSDVPWGVLLSG-GLDSSLVASICARNVARRS-----SSFPKLHSFTVGLEGSPDLKAAKEVADFLGTIHHAYTYTLEEGADAIREVIRSVETYDLTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAQEFYDETVDKISRLHMYDCLRANKSMSAWGVEPRCPFLDDDFLQVAMNLDPKEKMIVKGPGVSKEDTRIEKWAIRKAFDTPDDPYLPDNILWRQKEQFSDGVGYGWVDSLKDIAEREVSDQMFANAANRYPHNTPTTKEGYRYRMIFEEIFPGEAAERTVPGGKSIACSTERAMNWDASFALRADPSGRC-AGVHDSAYDDNFVADTVIESDEPSSKKA 596          

BLAST of mRNA_P-fluviatile_contig65.13156.1 vs. uniprot
Match: K0S197_THAOC (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0S197_THAOC)

HSP 1 Score: 652 bits (1681), Expect = 6.640e-228
Identity = 329/570 (57.72%), Postives = 408/570 (71.58%), Query Frame = 0

Query:   22 KNAIPVPFSLRHRGPDWSGYEIVTVSEKR----RHGIGHERLSIIDPESGAQPLFSRDGQVILAANGEIYNYQDILA----DEEPLTGSDCEAIIPLYLKKGG--DFLNDLRGMFAFFIYDKRDDSFFVARDHVGIVPLYIGWGDDGSVWLSSVCKEALISGCRTLKAFPPGHTYSSK---TGEFTQWYSPVWRSIKEH----PTRRVDLTRIRDTFTQAVAKRMMTDVPWGVLLSGRGLDSSLVASVACRYMRDHPDSALFSSFPKIHSFSVGLANSPDLKAAEEVARFLDTVHHSYVYTVQEGLDAIREVVRMIETYDTTTIRAATPMFLMSRKIKAMGVKMVLSGEGADELLGGYLYFHKAPTPQEFFEETRDKVLALHQFDCARANKSTAAWGVEARVPFLDVDFIEEVMMTSPEDKMIVPG--------KIEKYILRKAFDTPDDPYLPQHILYRQKEQFSDGVGYNWIDDLRAKAEAEVSDTQFQHRANRFPRDPPATKEAYLYRAVFEELFPSQAAVETVPMGKSIACSTSRALEWDESFKNRADCSGRAVAGVHDDAYGADF 566
            K  I     LRHRGPDWSGY +V   E +     HGI HERL+I+DPESG+QPL SR G +I+AANGE+YNY+++ +      EP+TGSDCE +IPL+ + G   +  N LRGM++  +YDK  D++F+ RD+VG  PLYIGWG+DGSV+++S  K  L++ C   + FPPGH YS+K    GEF +WY P W    +     P  +     +R  F Q+V +RMM+DVPWGVLLSG GLDSSLVAS+  R +         SSFPK+HSF+VGL  SPDL AA+ VA FL T+HH+Y YT++EGLDAIR+V+R +ETYD TTIRA+TPM+LMSRKIKAMG+KMVLSGEGADE+ GGYLYFHKAP  QEF++ET DK+  LH +DC R NK+ +AWGVE R PFLD DF++  M   P+DKMIV G        +IEKY +RKAFDTPDDPYLP  IL+RQKEQFSDGVGY W+D L+  AE EVSD  F + ANR+P + P TKE + YR +FEELFP +AA  TVP GK+IACST RA+ WD SF  RAD SGR+ AGVHD +YG  F
Sbjct:   19 KELIQCSSKLRHRGPDWSGYVVVEKDESKGISMNHGIAHERLAIMDPESGSQPLVSRGGNLIVAANGEVYNYKELYSALSTPYEPMTGSDCEVMIPLWDELGPSPELPNKLRGMYSVIMYDKASDTYFIFRDNVGKTPLYIGWGNDGSVYVASEMKS-LVNECTRFQNFPPGHCYSNKGPNPGEFVRWYKPNWAPEMKPGLPPPKEKFQADVLRHAFEQSVIRRMMSDVPWGVLLSG-GLDSSLVASICARNIARRS-----SSFPKLHSFTVGLEGSPDLIAAKRVADFLGTIHHAYTYTIEEGLDAIRDVIRSVETYDLTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNEQEFYDETVDKISRLHMYDCLRCNKAMSAWGVEPRTPFLDADFLQVAMNIDPKDKMIVKGPGVSKEDTRIEKYAIRKAFDTPDDPYLPDDILWRQKEQFSDGVGYGWVDSLKEVAEREVSDQMFANAANRYPHNTPTTKEGFRYRMIFEELFPGEAAERTVPGGKTIACSTERAMGWDASFAQRADPSGRS-AGVHDSSYGETF 580