prot_P-fluviatile_contig61.12843.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A6H5JRV7_9PHAE (UBX domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JRV7_9PHAE)

HSP 1 Score: 548 bits (1412), Expect = 2.360e-179
Identity = 489/944 (51.80%), Postives = 541/944 (57.31%), Query Frame = 0

Query:    1 MSSPLALDATSLEDLVALARAQGIHVEDETDRDSVVGLLQASAVPDVVSPPSPXXXXXXXXXXXXXASRSGDSVGLEAFLEGGGGLSFMPP-----------------PSPPRPFSPMTVGSPLPPAVARAXXXXXXXXXXXXXXXXXXXXVAQAAVLPSQQLSDIWAL-GEMRRP-HGDTSSSVGAEVGTEASRTTSRGGGFRGSAAGDDGNXXXXXXXXXXXXSDRGVDEDDMEAFSYHSSLSTGAPVAAAAAGRVRSRLAEIDR-------------EVAAEERREALEMFMAVTQVESSAAAAQFLDATDWSPDAAINLFLESGGDAPSATSAVGGFGSGSPGMNAVARAGXXXXXXXXXXARSG-AETRRWPGDSRGSGYG-----DXXXXXXXXXXXXXXXXXXXXPLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARXXXXX-SAGDHDPLYPGGFSEDGIRAPLPAMTDRLIGGIGPGGDSLGTGAGGLLRXXXXXXXXXXXGPVGG---AEEKDHTDWIFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSIDQVKAPALKTIGLGNITHHEEGVYSGRASPDTSVGTYGTSGKGG-GGASYTKDSAYESFGSEEYVYDDEDDVGPQESSFDGDDNNTSSF--------------------TAAARSPTHTSAAGSGHGGVDGARTPAATEEGQSEGXXXXXXXXXXXXXX---------AELLGPWGRGTPGDELRAQQAASEAEDDEEGVPDEPEAGPGVTQVLFRLPGSGTIARRFWLRDHVSLLFRFVRTKGEGASTRPFDLLSGHPPSSLSPSKVLTLEAANLGNSAIMVRWL 872
            MS+  + D TSLEDLVALA+AQG+HVEDETDRD VVGL+QAS V D+                      SGD+                                   PSPPR  SPM+ GSPLP A                               P Q+LSDI AL G + RP +GD SSSV  EVG+   R                G XXXXXXXXXXXX   G  + DMEA S                                         E   EERREALE FMA TQV SS +AAQFLDATDWSPDAAINLFLESGGD  +             G  A AR+G          A  G  + + +  D   +  G     D                    P   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  XXXXX S G+ D LYPGGFSEDG+RAP+PA TDRLIGG G  G            XXXXXXXXXXX    G    EE+D+ DWIF  PEGLSFPS+FLETRQICK QKKWLM    DH+EFASHRLN+DVWSNETILTLLRGNFIFWQRNK LRQARYY++KYNLEGQVLPHTAILDPRTGA LLKVVGFVEPEDLSMALVEFLENNSIDQ                 + G  S R SPD SVG Y + G+GG G AS ++DSAYES+GSEEYVY+D+DD+G Q SSFDGDDN+T++                     T  + SP+  + AGSG         P+A        XXXXXXXXXXXX           A L+GPWGRGTP DELRA+ A      +   VP EP+AG GVTQV FRL G GTIARRFWL D VSLLF+FVRT GE A+TRPFDLLSGHPPSSL  SKV TLEAANL NSAIMVRWL
Sbjct:    1 MSAVPSFDTTSLEDLVALAQAQGLHVEDETDRDHVVGLVQASVVADLAE--------------GELTGGSGDASAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTPSPPRHLSPMSFGSPLPGAP------------------------------PPQRLSDILALDGMIGRPSYGDASSSVCTEVGSRTGR----------------GXXXXXXXXXXXXXXXXG--DSDMEASSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEDLTEERREALETFMAFTQVGSSVSAAQFLDATDWSPDAAINLFLESGGDTAAVXXXXXXXXXXXAGYGAPARSGGARRWPSVGGAAGGYGDGQSYVSDDNHNEEGADDHDDAGANPFTALGELPGLVIPPAPAGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSPGEQDHLYPGGFSEDGVRAPIPARTDRLIGGGGVLGXXXXXXXXXXXXXXXXXXXXXXXXXXAGRSSVEERDNMDWIFKAPEGLSFPSDFLETRQICKEQKKWLM----DHQEFASHRLNKDVWSNETILTLLRGNFIFWQRNKALRQARYYVDKYNLEGQVLPHTAILDPRTGAQLLKVVGFVEPEDLSMALVEFLENNSIDQ-----------------DGGGVSERTSPDNSVGMY-SGGRGGRGDASLSRDSAYESYGSEEYVYEDDDDLG-QGSSFDGDDNSTANSHASPGYHPQNTPNAKPSPPATTCSSSPSPLTVAGSGRSSPS---RPSAXXXXXXXXXXXXXXXXXXXXGGTGAIGAAAGAGLVGPWGRGTPEDELRAEGAGQGVLGEPVDVPQEPDAGNGVTQVQFRLAGGGTIARRFWLNDRVSLLFQFVRTTGEEAATRPFDLLSGHPPSSLLGSKVSTLEAANLSNSAIMVRWL 856          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: D8LLD2_ECTSI (UBX domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLD2_ECTSI)

HSP 1 Score: 530 bits (1366), Expect = 6.790e-175
Identity = 412/653 (63.09%), Postives = 447/653 (68.45%), Query Frame = 0

Query:  267 QVESSAAAAQFLDATDWSPDAAINLFLESGGDAPSATSAVGGFGSGSPGMNAVARAGXXXXXXXXXXARSGAE-TRRWPG-DSRGSGYGDXXXXXXXXXXXXXXXXXXXX-------PLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARXXXXXSAGDHDPLYPGGFSEDGIRAPLPAMTDRLIGGIGPGGDSLGTGAGGLLRXXXXXXXXXXXGPVGG---AEEKDHTDWIFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSIDQVKAPALKTIGLGNITHH--------EEGVYSGRASPDTSVGTYGTSGKGG-GGASYTKDSAYESFGSEEYVYDDEDDVGPQESSFDGDDNNTSSFTA-----------AARSPTHTS---------AAGSGHGGVDGARTPAATEEGQSEGXXXXXXXXXXXXXX------AELLGPWGRGTPGDELRAQQAASEAEDDEEGVPDEPEAGPGVTQVLFRLPGSGTIARRFWLRDHVSLLFRFVRTKGEGASTRPFDLLSGHPPSSLSPSKVLTLEAANLGNSAIMVRWL 872
            +V SS +AAQFLDATDWSPDAAINLFLESGGDA                        XXXXXXXX X        R WP      +GYGD     XX                     L      XXXXXXXXXXXXXXXXXXXXXXXXXXX  XXXXXS G+ D LYPGGFSEDG+RAP+PA TDRLIGG G  G            XXXXXXXXXXX    G    EEKD+TDWIF  PEGLSFPS+FLETRQICK QKKWLMVNIQDH+EFASHRLN+DVWSNETILTLLRGNFIFWQRNK LRQARYY++KYNLEGQVLPHTAILDPRTGA LL+VVGFVEPEDLSMALVEFLENNSIDQVKAPA++TIG+GNI HH        + G  S R SPD SVG Y + G+GG G AS ++DSAYESFGSEEYVY+D+DD+G Q SSFDGDDN+T++  A           A  SP  T+          AGSG        + AAT       XXXXXXXXXXXXXX      A L+GPWGRGTP DELRA  A      +   V  EP+AG GVTQV FRL G GTIARRFWL+D VSLLF+FVRT GE A+TRPFDLLSGHPPSSL  SKV TLEAANL NSAIMVRWL
Sbjct:   27 EVGSSVSAAQFLDATDWSPDAAINLFLESGGDAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYXXXXXXXGARGWPNVGGAAAGYGDGQSDDXXNEEGADDRDDVGANPFSALGELPGLVIPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSPGEQDHLYPGGFSEDGVRAPIPARTDRLIGGRGVLGGXXXXXXXXXXXXXXXXXXXXXXXXXAGRSSVEEKDNTDWIFKAPEGLSFPSDFLETRQICKEQKKWLMVNIQDHQEFASHRLNKDVWSNETILTLLRGNFIFWQRNKALRQARYYVDKYNLEGQVLPHTAILDPRTGAQLLRVVGFVEPEDLSMALVEFLENNSIDQVKAPAVRTIGMGNIAHHGYNQTPGKDGGGVSERTSPDNSVGMY-SGGRGGRGDASLSRDSAYESFGSEEYVYEDDDDLG-QGSSFDGDDNSTANSHASPGYHPQTTPNAKPSPPATTRSSXXXXLTVAGSGRS-TPSRPSAAATXXXXXXXXXXXXXXXXXXXXXXXXXXGAGLVGPWGRGTPEDELRAAGAGEGVLGESVEVLQEPDAGNGVTQVQFRLAGGGTIARRFWLKDRVSLLFQFVRTTGEEAATRPFDLLSGHPPSSLLGSKVSTLEAANLSNSAIMVRWL 676          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A7S2FLK4_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2FLK4_9STRA)

HSP 1 Score: 153 bits (387), Expect = 1.410e-35
Identity = 139/441 (31.52%), Postives = 196/441 (44.44%), Query Frame = 0

Query:  433 GIRAPLPAMTDRLIGGIGPGGDSLGTGAGGLLRXXXXXXXXXXXGPVGGAEEKDHTDWIFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQV-LPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSIDQVKAPALKTIGLGNITHHEEGVYSGRASPDTSVGTYGTSGKGGGGASYTKDSAYESFGSEEYVYDDEDDVGPQESSFDGDDNNTSSFTAAARSPTHTSAAGSGHGGVDGARTPAATEEGQSEGXXXXXXXXXXXXXXAELLGPWGRGTPGDELRAQQAASEAEDDEEGVPDEPEAGP-GVTQVLFRLPGSGTIARRFWLRDHVSLLFRFVRTKGEGASTRPFDLLSGHPPSSLSPSKVLTLEAANLGNSAIMVRW 871
            GIRAP  A  D LI      G S G   GGL R           G     ++ + +DW+F  P+ +    + +E R++ K Q KWL+VN+QD E F SH+LN+D W N+TI  LL  +FIFWQR+ T +  R ++  Y L   V LPH AIL P TG+ L    GF++P+DLS+ALVEF+E N ++ +  P  +  G            +G A    S  TY          S ++D+  ES   E +V+DD D+      +   +D+ T   ++A+   + TS                 +EE +                  + L       P  EL AQ             P EP  G    TQV  RL    T+  RF L   V  ++  V  K   A+ R F L +  P   L P    TL  A L N++I++RW
Sbjct:  193 GIRAPQAAFRDTLI------GPSSGMMMGGLGRG----------GMASSTDDLEASDWMFAPPDSVK--GDIIEARRMAKEQGKWLLVNLQDVEIFESHQLNRDTWKNDTIQALLETSFIFWQRSSTSQAGRDFLRLYGLSADVCLPHIAILGP-TGSQLWSRTGFIQPDDLSIALVEFMEANDLNAMTKPKERHFGRA----------AGGAGSSRSSATYD--------GSKSRDANDES--GERHVFDD-DEALQAALAMSLEDDVTEDRSSASEKKSATS-----------------SEEAKDHSTEIVPDSLDTQTRHRDWL-------PSGELSAQS------------PIEPAPGTLNSTQVRLRLANGTTMVYRFLLDALVQSVYAVVLDKDPEAAERAFTLQTAFPVVQLLPLASKTLAEAGLSNTSIVMRW 557          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A836CHR7_9STRA (UBX domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHR7_9STRA)

HSP 1 Score: 139 bits (349), Expect = 5.870e-31
Identity = 64/146 (43.84%), Postives = 94/146 (64.38%), Query Frame = 0

Query:  481 GAEEKDHTDWIFNIPEGLSFPSNFLETRQI-----------CKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNS 615
            G  E + T+W+F  P  +   S+F +TRQ            C+ +K+W+MVNIQD   FASHRLN+DVWS+ET+  L+  +F+FWQR  T   A+ + E+Y++    LP TAI+DPRTG  L++V GF+ PED+S  ++E +E+ S
Sbjct:  193 GGAEGESTEWLFPPPTDMIERSSFSDTRQATELSYARDAIACRQRKEWIMVNIQDRSVFASHRLNRDVWSDETVRALVSESFVFWQREVTSEDAQGFCERYSVRSDALPVTAIIDPRTGGMLVRVEGFISPEDMSSLIMEIMESRS 338          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A7S2FU61_9STRA (Hypothetical protein (Fragment) n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2FU61_9STRA)

HSP 1 Score: 127 bits (319), Expect = 1.930e-29
Identity = 64/140 (45.71%), Postives = 85/140 (60.71%), Query Frame = 0

Query:  488 TDWIFNIPEGLSFPS--NFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSIDQVKAPALK 625
            +DW+F  P  L+  +  +  E R   K  +KWL+VN+Q  + F SHRLN+D WSNETI TLL  +F+FWQR+        YI  Y +    LPH AI+ P TGA L    GF++P++LSMALVEF+E N +D    P  K
Sbjct:   23 SDWMFPPPRNLTCAAGGDVREARNQAKDARKWLLVNVQSVDIFDSHRLNRDTWSNETIQTLLDSSFVFWQRSSGCSAGADYIRLYKVSHDDLPHIAIIGP-TGATLWSQTGFIQPDELSMALVEFIEQNDLDATTLPKPK 161          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A7S2WDF0_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WDF0_9STRA)

HSP 1 Score: 111 bits (277), Expect = 5.740e-23
Identity = 108/368 (29.35%), Postives = 159/368 (43.21%), Query Frame = 0

Query:  508 RQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSIDQVKAPALKTIGLGNITHHEEGVYSGRASPDTSVGTYGTSGKGGGGASYTKDSAYESFGSEEYVYDDEDDVGPQESSFDGDDNNTSSFTAAARSPTHTSAAGSGHGGVDGARTP--AATEEGQSEGXXXXXXXXXXXXXXAELLGPWGRGTPGDELRAQQAASEAEDDEEGVPDEPEAG-PGVTQVLFRLPGSGTIARRFWLRDHVSLLFRFVRTKGEGASTRPFDLLSGHPPSSLSPSKVLTLEAANLGNSAIMVRWL 872
            +Q  K ++KWL+VN+Q  + F SH+LN+D WS+ETI  LL  +FIFWQR+      R Y+  Y +  + LP  AI+ P TG  L    GF+ P +LS  ++EFLE NS+D   AP ++          +  +      P ++     T                          D  D++G   S+ D +       + AA  P      G   G  +G+ TP  A +EE Q+                A  L P  + T    LR                +EPE G P  T++  RL    T+ RRF+L D ++ ++  V  + E AS+R F L        L      TLE  +L NS I++RW+
Sbjct:   19 KQRAKEERKWLLVNLQALDNFQSHQLNRDTWSDETIQALLESSFIFWQRSSASEAGRQYLNLYRVAPEDLPEIAIIGP-TGIKLFSHSGFLPPAELSGMVLEFLEQNSLDSKDAPKMRQRKRSTSGGEDLALPV----PSSTASAQRT--------------------------DLADELGL--SAEDLELERALQMSMAAAEPL-----GDERGDAEGSPTPDLAQSEEEQA--------LSSEKGAAATHLPPGFKDT----LR----------------NEPEKGVPNTTRLQLRLADGRTLVRRFFLDDKIAAIYAVVCAEVEEASSRAFTLQPPMNSLELLSVADSTLEDCDLTNSKIIMRWV 320          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A7S1CF00_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CF00_9STRA)

HSP 1 Score: 106 bits (264), Expect = 2.780e-21
Identity = 68/193 (35.23%), Postives = 97/193 (50.26%), Query Frame = 0

Query:  429 FSEDGIRAPLPAMTDRLIGGIGPGGDSLGTGAGG------LLRXXXXXXXXXXXGPVGGAEEKDHTDWIFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNS 615
              +DG+RAP      RLI    PG  ++G  AG       L+R           G         +   IF  P  +   ++F         QKKWL+VN+QD EEFASH LN+D WS+++I  ++RGNFIFWQ        + Y + Y     V P  AI+DPRT   ++++ GF+EP  L+  L +FL+ +S
Sbjct:   13 LDDDGVRAPDEVKRMRLIDAPPPG--TIGGYAGPRSVAPRLMRNLKQEAQARGLG--------GNLASIFRPPIDIMEDTDFDNAVASANLQKKWLLVNMQDDEEFASHMLNRDTWSDDSIKEIVRGNFIFWQTFHKGAMGQRYTQFYR--PTVYPLIAIIDPRTKQKVMEITGFIEPRVLAQRLADFLDGHS 193          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: I0YL99_COCSC (UBX domain-containing protein n=1 Tax=Coccomyxa subellipsoidea (strain C-169) TaxID=574566 RepID=I0YL99_COCSC)

HSP 1 Score: 105 bits (263), Expect = 2.190e-20
Identity = 62/188 (32.98%), Postives = 93/188 (49.47%), Query Frame = 0

Query:  431 EDGIRAPLPAMTDRLIGGIGPGGDSLGTGAGGL-LRXXXXXXXXXXXGPVGGAEEKDHTDWIFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSID 617
            E+ +R PLPA+ DRL G       + G+ +    ++              GG   +     +F  P  L F     E +     Q +WL++N+Q + EFASH+LN+D WS++T+ T++RG+FIFWQ N            Y L    LP T ++DP TGA      G +EP+ L   LV FL+++  D
Sbjct:   72 EEHVRPPLPAVRDRLYGETFTSRGASGSQSHQQEVQAFRDFKAETSKSRAGGKSNQPGLAGLFEPPHDLMFKGTLEEAKAAALEQSRWLLINVQSNSEFASHQLNRDTWSDDTVKTIIRGSFIFWQVNDASENGSKVKAFYRLTE--LPVTLVIDPVTGASPKAWTGAIEPQRLIEELVPFLDHDIHD 257          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: A0A7S1HAU8_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1HAU8_HEMAN)

HSP 1 Score: 106 bits (264), Expect = 2.450e-20
Identity = 58/161 (36.02%), Postives = 89/161 (55.28%), Query Frame = 0

Query:  478 PVGGAEEKDH-TDWIFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQVLPHTAILDPRTGAPLLKVVGFVEPEDLSMALVEFLENNSIDQV-KA-PALKTIGLGNITHH 635
            PV   E KD   + +F  P  L F  NF   R+  +  KK+ +VNIQ H+EFASH LN+D W +E +  ++R +F+FWQ+       R+Y+  Y      LP   I+DP TGA L ++ G+  PED+   L  FL++++  ++ KA P    +G+    +H
Sbjct:  117 PVSTGESKDTGMEMLFPPPADLMFQGNFDALRKGAEEDKKYCLVNIQKHDEFASHALNRDTWKSEQVCQVVRSSFVFWQQQFESISGRHYLSLY--ASHDLPVVDIIDPMTGALLDRIEGYTTPEDMVERLTRFLDSHTWGKMGKAMPTQVAMGMSQAQYH 275          

BLAST of mRNA_P-fluviatile_contig61.12843.1 vs. uniprot
Match: C5LRI8_PERM5 (UAS domain-containing protein n=1 Tax=Perkinsus marinus (strain ATCC 50983 / TXsc) TaxID=423536 RepID=C5LRI8_PERM5)

HSP 1 Score: 96.3 bits (238), Expect = 7.510e-20
Identity = 44/99 (44.44%), Postives = 63/99 (63.64%), Query Frame = 0

Query:  491 IFNIPEGLSFPSNFLETRQICKAQKKWLMVNIQDHEEFASHRLNQDVWSNETILTLLRGNFIFWQRNKTLRQARYYIEKYNLEGQV-LPHTAILDPRTG 588
            +FN+P+GLS P  F E     + +K+WL+VNIQD+E F SH LN+D+W    +  LL+ +FI WQR+K   +A  Y+  Y  + +  LP   +LDPRTG
Sbjct:    3 LFNLPKGLSSPLPFAEVIAKARCEKRWLIVNIQDNENFVSHSLNRDIWKQSMVQDLLKTSFILWQRSKEEAEAVQYLTYYCKDDEAPLPLVHVLDPRTG 101